Garlic Mustard Report 2013 - The Aldo Leopold Foundation
Transcription
Garlic Mustard Report 2013 - The Aldo Leopold Foundation
Garlic Mustard Report 2013 9/18/2013 Steven Bachleda Cameron Bushong Emily Iehl Dakota Johnson Lyndsey Mittelstaedt Leann Wolthusen Cooper Kohlman Alanna Koshollek Land Stewardship Staff The Aldo Leopold Foundation -1- Table of Contents Introduction .......................................................................................................................................... - 1 Natural History of Alliaria petiolata ...................................................................................................... - 1 Implications ........................................................................................................................................... - 6 Garlic Mustard Control on the Leopold Memorial Reserve ................................................................... - 6 Methods and Materials ........................................................................................................................ - 7 Overview of Treatment Program .......................................................................................................... - 7 Management Units, Transecting, & Control Methods .......................................................................... - 7 Mapping and Data Collection Protocol ................................................................................................. - 9 Levee Road and Areas Outside of Management Units ....................................................................... - 12 Results................................................................................................................................................. - 12 Discussion ........................................................................................................................................... - 15 Overview of 2013 Garlic Mustard Season ........................................................................................... - 15 Changes in protocol regarding maps and mapping ............................................................................ - 19 Analysis of new equipment ................................................................................................................. - 21 Suggestions and potential changes in protocol for 2014 .................................................................... - 21 Notes on Management Units .............................................................................................................. - 23 Appendix A ......................................................................................................................................... - 26 Works Cited ........................................................................................................................................ - 29 - Photos Photo 1. Garlic mustard seedlings………………………………………………..………………………………………………………...1 Photo 2. Garlic mustard basal rosettes. ………..............................................................................................2 Photo 3. Garlic mustard flowering stalks…………………………………………………………………………………..…………....2 Photo 4. Garlic mustard flowers………………………………………………………………………………………………………..…...3 Photo 5. A forest understory taken over by garlic mustard……………………………………………………………..……...3 Photo 6. Garlic mustard siliques………………………………………………………………………………………………………….....4 Photo 7. Viable garlic mustard seeds……………………………………………………………………………………………………...4 Photo 8. The 2010 Stewardship crew transecting a unit in the floodplain forest. …………………………..……...8 Photo 9. The 2013 interns transecting a unit with dense vegetation. …………………………………………………....8 Photo 10. A screen shot of our PDA garlic mustard program..…………………………………….……………………....10 Photo 11. A garlic mustard plot classified as “dense”…………….………………………………………………….…….…...11 Photo 12. A garlic mustard plot classified as “scattered”.……………………………………………………………..……...11 Photo 13. A garlic mustard plot classified as “very scattered” ……………………………………………………..….…...11 Photo 14. Stewardship intern Steven Bachleda transects through high water in the floodplain forest....18 Photo 15. A paper map in comparison to the map shown by the PDA garlic mustard program….………...20 Figures Figure 1. Expansion of a garlic mustard population……………………………..…………………………………………………5 Figure 2. The range of garlic mustard in Wisconsin as of 2011..………………………………..……………………………5 Figure 3. Diagram used to determine population diameter.………………………..……………………….………………11 Figure 4. Timescale of garlic mustard management at ALF from 2003 through 2013…………………..…….…12 Figure 5. Percentage of plots within each diameter size class.……………………………………………………..………13 Figure 6. Total number of acres occupied by garlic mustard populations from 2003 to 2013.………………13 Figure 7. Yearly totals of gallons of bagged plants and gallons of herbicide used from 2003 to 2013…..15 Tables Table 1. Hours spent in each management unit during initial visits and revisits in 2013.………………………14 Table 2. Chemical Control (Initial Visits) of Garlic Mustard by ALF Land Stewardship crew and volunteers, LMR, Spring 2013…………………………………………………………………………………………………………..……26 Table 3. Revisiting Garlic Mustard Populations by ALF staff, LMR, 2013.…………………..………………………....27 Table 4. Total garlic mustard removal by ALF staff and volunteers, 2003-2013…………………………………….28 Table 5. Number of garlic mustard populations and total acres.…………………………………………..………………28 Introduction Garlic mustard (Alliaria petiolata) is a severely aggressive invasive weed that has spread quickly through North American forests since its introduction to the United States in 1868. Since it was first discovered on Long Island, New York, this member of the mustard family (Brassicaceae) has migrated to 37 states in the U.S. and five provinces in Canada (Munger 2001, USDA 2013). Supposedly cultivated for its use in cooking and medicine by early American settlers, garlic mustard now invades a variety of ecosystems, including those on the Leopold Memorial Reserve. This year, the 2013 Land Stewardship Interns continued to monitor and treat populations of garlic mustard on 300 acres of the Reserve. Natural History of Alliaria petiolata Garlic mustard is an obligate biennial herb, which means that the plant has a two-year life cycle. According to Nuzzo (2000), the life cycle starts when a seed germinates and the seedling plant (Photo 1) emerges in early spring. Although germination often occurs well before the appearance of native spring ephemerals, new seedlings have been observed by the Aldo Leopold Foundation Stewardship Crew throughout the entire growing season. This means that seeds do not have a very limited window in which to germinate—they may sprout at any time during the summer. Photo 1. Garlic mustard seedlings. Note the bright green color, delicate form, and clear scalloping on margins of leaves. Seedlings may grow as a single plant or in carpets, as shown in the photo. -1- During its first year, garlic mustard germinates as a seedling and then transforms into a basal rosette. The leaves of the rosette are dark green and kidney-shaped with scalloped edges, 6-10 centimeters in diameter (Photo 2). The plant does not produce seeds in the first year and will lie dormant as a rosette through the winter months. Basal rosettes can be managed with chemical treatment or by hand pulling, but first year plants are less of a priority for control than second year plants. www.eddmaps.org msue.anr.msu.edu Photo 2. Garlic mustard basal rosettes. The leaves on this life stage are larger, rounder, darker green color, and have deeper venation. Multiple stems grow from one root system. In its second growing season, garlic mustard rosettes reemerge and produce flowering stalks that reach 2-4 feet in height (Photo 3). Manual or chemical control is imperative before these stalks go to seed. Second year plants often produce more than one flowering stem from a single basal rosette, with some single plants producing up to 12 separate stems that continue to grow throughout the summer. White, cross-shaped flowers typically bloom 6-10 weeks after plants come out of dormancy (Photo 4). Pollination occurs as long as flowers bloom. Photo 3. Garlic mustard flowering stalks. The architecture of the mature plant is taller and more linear than the basal rosette, but multiple stalks may still spring from one root system. The leaves are triangular in shape with sawlike teeth. You may encounter mature garlic mustard plants singly or in clumps and with or without flowers. wisconsin.sierraclub.org mda.state.mn.us -2- The Virtual Nature Trail at Penn State New Kensington (2013) describes the different methods of garlic mustard pollination and their implications as follows: “The flowers of garlic mustard are either pollinated quite non-specifically by a variety of insects (solitary bees, a variety of flies, and on rare occasions, honeybees or bumblebees) or they can self-pollinate if these insect interactions do not occur. The seeds of the pollinated plants have been shown to be more vigorous than those produced by the self-pollinated plants. The ability to self-pollinate, though, does confer a great advantage on the survivability of an invading population founded by a single, established individual. Garlic mustard also has a slender, white taproot from which adventitious buds that can form flower stalks can arise. This root budding ability further adds to the difficulty of population control of these invasive plants.” The opportunistic reproduction of garlic mustard contributes heavily to its ability to invade new territory (Photo 5). All of these reproductive strategies must be taken into consideration when managing the species. ecga.org Photo 4. Garlic mustard flowers. Flowers are small, white, and have four petals. A single stalk produces many flowers. beyondturf.com Photo 5. The opportunistic reproduction of garlic mustard allows the species to take over entire forest understories and other niches in different communities. At the Aldo Leopold Foundation, the most efficient strategy to manage garlic mustard populations is to prevent the majority of seeds from replenishing the seed bank. After pollination, garlic mustard flowers produce green siliques that contain maturing seeds (Photo 6).The number of seeds produced by each plant can vary from a few hundred seeds up to 7,900 seeds or more from a large, multi-stemmed plant. When the seeds have matured and become viable, the siliques burst open and drop the seeds to the ground (Photo 7). The seeds remain dormant until 50 to 105 days of cold stratification trigger the seed to germinate. If not subjected to an adequate cold period, the seeds may remain viable in the seed bank for five years or more, germinating only when conditions are conducive to the success of the plant (PSU New Kensington Virtual Nature Trail 2006). -3- florafinder.com Photo 6. Garlic mustard siliques, which form after pollination and contain maturing seeds. countrycrone.com Photo 7. Viable garlic mustard seeds. When the seeds mature, the siliques stop growing, turn brown, and split open to release the seeds. The seeds’ capacity to stay viable for many years contributes to the spread of garlic mustard across the continent. They can be transported through a number of possible vectors in their five year lifespan and can take root in places far from the parent plant. Most commonly, garlic mustard seeds are picked up and transported by animal feet and fur. The treads of shoes and tires also prove effective in the spread of garlic mustard, especially by off-trail hikers and roadside mowers. Flash floods or runoff from a rainstorm can lift the seeds and carry them with the current to new locations. However, in slow or standing water, the shape and weight of the seed usually causes seeds to sink instead of float, which inhibits much other water-based transportation. Similarly, although transportation by wind cannot be ruled out, the physical shape of garlic mustard seeds is not particularly aerodynamic. Figure 1 depicts the aggressive regeneration of a single garlic mustard plant by seed. Combined with the easy transport of seeds by people and animals, even a couple years of no treatment can contribute to significant increases in population sizes and time required to get control of the species. Other innate characteristics of garlic mustard also contribute to its aggressiveness as an invasive species. Ironically, some of these characteristics may have played a role in the plant’s appeal to humans as a culinary and medicinal aid. For example, the plant’s garlic taste and smell often repel North American predators. Even white-tailed deer avoid the plant, preferring to graze upon better-tasting native species. The plant also produces antifungal chemicals that secrete into the soil and, on this continent, “disrupt associations between mycorrhizal fungi and native plants, suppressing native plant growth” (WDNR 2013). The suppression of native plant -4- growth contributes significantly to the destabilization of any ecosystem; including those that garlic mustard invades in North America. Single seedling Year 2 Year 3 Year 4 Year 5 Year 6 Figure 1. Expansion of a garlic mustard population. In year 1 a single seed germinates and a garlic mustard plant begins its life cycle. In year 2, the single plant matures and disperses seed. In year 3, seeds from the parent plant germinate, multiple basal rosettes are produced, and a population is formed at .5m-1m. In year 4, the basal rosettes mature and disperse seed, extending the population to >2m with both first and second year plants. In year 5, both first and second year plants are present. If left unchecked, the population will begin to exponentially grow from this point forward, while adding thousands of seeds to the seed bank. This scenario creates a population which will need to be managed for at least 7 years. If the population is detected prior to year 4, management efforts will be decreased. Originally native to northern Eurasia from England to the western edge of the former Soviet Union, garlic mustard can now be found in forests and forest edge habitats, along roadsides and stream banks, and in disturbed and urban areas of North America. Garlic mustard is found in many locations throughout southern and central Wisconsin (Figure 2). It is possible that there are additional unrecorded areas in the state where garlic mustard grows. dnr.wi.gov Common communities in which it can be found Figure 2. The range of garlic mustard in include oak savannas, prairies, and both floodplain and upland forest. It is rarely found in pine-dominated Wisconsin as of 2011. The only counties with no records of the plant are Bayfield, Washburn, ecosystems, but the plant will eagerly colonize areas Eau Claire, Menominee, and Shawano counties. where conifers exist in less abundance (Munger 2001). Garlic mustard will also tolerate most soil types from sand to clay, but cannot tolerate very acidic soils, such as peat. In addition to its ability to adapt to a variety of soils, the plant -5- does well in a wide range of light. It can be found in partial shade to full sun, although it seems to do best with half shade. These conditions can be found readily on the Leopold Memorial Reserve, where Aldo Leopold Foundation Staff first started controlling garlic mustard 18 years ago. Implications Outside of its native range, garlic mustard has spread quickly and relatively unchecked. Although efforts to educate the public about the transport and impact of this weed in state parks, state natural areas, and privately-owned land abound, new populations of garlic mustard continue to become established in previously uninfected areas at an alarming rate. Due to its numerous opportunistic reproductive strategies, populations that are neglected for even one season can add a significant number of seeds to the local seed bank, requiring that monitoring and treatment continue for many more years. In most cases, federal and state governments cannot take an active approach to managing garlic mustard due to its aggressive nature. Staff members of the Wisconsin Department of Natural Resources, who manage public land near the Aldo Leopold Legacy Center, explain that the personnel requirements and funding to control garlic mustard are unavailable to large management areas. Without these resources, combating garlic mustard is impossible. In effort to overcome deficiencies in staff and funding, some research has been conducted about the possibility of releasing a European weevil as a biological control (Blossey et al 2001). In Europe, 70 known species of insects and seven species of fungi prey upon different parts of Alliaria petiolata and keep populations in check. However, in the 20 years that this research has been continuing, no plan of action has yet been implemented for the release of the weevil into the United States or Canada, and garlic mustard continues to spread. Garlic Mustard Control on the Leopold Memorial Reserve In an effort to preserve and restore the integrity of native ecosystems on land owned by the Aldo Leopold Foundation, 18 generations of ALF Land Stewardship interns have developed an intensive approach to garlic mustard control. In 1996, interns started spraying and hand pulling garlic mustard around the Shack and other prominent areas of the Leopold Memorial Reserve. Each year, these areas of known garlic mustard populations grew in number and control efforts expanded across the LMR. In 1999, a tract of land called the Potter Preserve was donated to the Foundation and areas of this property were also incorporated into the initial stages of garlic mustard control. Interns used -6- early GPS technology to record the locations of populations and some flags were laid down to aid in the search for these known populations. It wasn’t until 2004 that garlic mustard treatment was extended into the floodplain forest, west of the Leopold Shack, which is now part of the 300 acres of the core management area on the LMR. Interns that year for the first time numbered and flagged each population they treated. Positions of the plots were recorded with GPS so that succeeding interns could retrace their footsteps and monitor the same populations year after year. After the Leopold Foundation decided to sell the Potter Preserve in 2009, the Stewardship Crew began an even more rigorous transecting and scouting effort of the LMR and since then treatment includes transecting all 300 acres. Through 10 years of data collection, the Leopold Foundation and other land managers can more clearly see the long-term effects of one of the biggest projects tackled by Land Stewardship interns each year. The protocol continues to evolve as changes in the garlic mustard populations occur in response to treatment, and this continuing work on the LMR is evidence that implementing a long term program for managing garlic mustard can be effective. Methods and Materials Overview of Treatment Program The management of garlic mustard at ALF is divided into two separate stages: initial visits and revisits. During initial visits, the Stewardship Crew transects through all 300 acres of the core management area of the Leopold Memorial Reserve and chemically treats all populations of garlic mustard. After initial visits, we re-transect all 300 acres and revisit each and every population to ensure thorough treatment. At the time of revisits in mid-June, garlic mustard is too far advanced in seed production to chemically treat and must be pulled by hand. All identified populations of garlic mustard on ALF property are marked with bright orange irrigation flags so that they can easily be located. Populations are given an identification number, which is written on the flag. The crew methodically covers all designated management units, transecting and searching for the previously made flags while simultaneously scouting for new populations. We also collect and record data for all populations so that their characteristics can be monitored over time. Management Units, Transecting, & Control Methods There are currently 15 designated management units for garlic mustard on the Leopold Memorial Reserve. Units vary in size and have been divided based on features of the landscape -7- for ease of treatment. Because certain constraints within the Stewardship Program prevent planning for an exact starting date for garlic mustard, Stewardship Crews traditionally work from areas with quality spring ephemeral vegetation to areas with less diverse vegetation to reduce the impacts of herbicide on non-target plants. Visuals of units and their locations, along with the average weekly progression for each unit, can be found in Appendix A on the map entitled Average Weekly Progression for Garlic Mustard. Crew members transect the entire area within each unit during initial visits and revisits to find new and existing populations. To transect, two to six crew members line up 5-10 feet apart from each other at one edge of the unit. The crew walks in a straight line (each crew member’s line parallel to the person next to him/her) to another side of the unit (Photo 8). Spacing between crew members depends on the density of vegetation; generally, more dense areas require crew members to be closer together as the line of sight is limited (Photo 9). The crew members on the outside edges of the transect are in charge of laying down pink flags to mark the extent of the transect. When the crew reaches their end boundary, they turn around about the flag line that was laid down by one outside crew member and walk back to the opposite boundary following the flag line along the way. The person who initially laid these flags down picks them up on the way back and the other person on the outside lays the new flag line, which marks the new transect border. This process is efficient and effective at finding existing populations, although it can often be time consuming. Photo 8. The 2010 Stewardship crew transecting a unit in the floodplain forest. The interns walk from one unit boundary to another in parallel lines 5-10 feet apart. In clear areas, like this meadow, crewmembers can increase the space between each other to cover more ground. Photo 9. The 2013 interns transecting a unit with dense vegetation. Since visibility is limited, crewmembers need to walk closer together in order to see each other and any garlic mustard that might be between them. -8- Upon encountering flagged or new populations of garlic mustard, first and second year plants are sprayed with a 1.5 - 2% RazorPro (glyphosate) and water solution. Tracer, a red foam dye, is also added to the solution to help increase visibility of sprayed areas. RazorPro takes at least a week to work before there is a visible result on the sprayed area. However, it only takes the chemical an hour to dry on the leaves of sprayed plants, at which point we can be confident that it will effectively kill the plant. We use 4-gallon Solo piston backpack sprayers outfitted with light-mist nozzles for all chemical application. We also transport large batches of mixed chemical to the field in a 35-gallon sprayer tank on the back of the Kubota, from which we can refill our backpack sprayers as needed. Since the chemical does not break down quickly after mixing, large batches of chemical can be made and stored for use throughout the week. To make a 1.5 - 2% Razor Pro solution in volumes equal to or less than 25 gallons, we mix 2 oz. of Razor Pro for each gallon of water (for example, to make 25 gallons of mixed chemical, combine 50 oz. of Razor Pro and 25 gallons of water). For volumes over 25 gallons, we mix 1.75 oz. Razor Pro per gallon. Other brands of glyphosate, such as Roundup, may require different mixing ratios of chemical to water, so be sure to check the label on your container. The manufacturer of Razor Pro also notes that “colorants or dyes used in spray solutions of this product may reduce performance, especially at lower rates or dilutions” (Razor Pro 2013). Be sure to check labels and instructions on anything you may add to your chemical mixture. It may also be helpful to write down your observations and fine-tune chemical treatment methods as you see results. Following the initial visits, all populations are visited a second time to hand-pull plants that were overlooked during initial visits. Garlic mustard has a slender, white, S-shaped taproot, which makes hand-pulling a fairly easy task in sandy soils. However, in richer or compacted soils, the plant can easily break off with the root remaining in the soil to produce another seedbearing plant. When hand-pulling, effort should made to pull the entire plant including the entire root to prevent re-growth. Garlic mustard seeds have the ability to continue to mature on the pulled plant, so any plant pulled is bagged and removed from the site. In addition to hand pulling, all bright orange irrigation flags need to be replaced during revisits. Crew members carry new flags and write the plot identification number and diameter on the flag with Sharpie chisel-tip markers. Flags need to be replaced yearly as weathering makes reading the information on the flag difficult. Mapping and Data Collection Protocol The mapping and data collection of the 2013 garlic mustard season was conducted according to protocol outlined in 2009. Each newfound population of garlic mustard is flagged with a bright -9- orange irrigation flag and numbered. We use a Personal Digital Assistant (PDA) with a Bluetooth GPS function to mark the plot location. The PDA contains an ArcGIS map of all the garlic mustard populations mapped from previous years. This allows crews to navigate to mapped populations, map new populations, and record the appropriate data for each point. The PDA also allows us to move previous GPS points that appear to be in the wrong location. After a day in the field, the PDA is connected to the computer and our data gets backed-up by ArcPad that extracts the field data. The software updates the ArcGIS maps with any new points that were created and also indicates which points have been treated and which have not. When a garlic mustard flag is found by the crew, the person who found it communicates the information on the flag to the rest of the crew. This information includes the plot identification number and the diameter of the population. The diameter that is written on the flag is the largest historical diameter of the population. All other crewmembers mark the identification number off of their field maps to indicate that the point has been visited. The crew member then walks and searches the entire area of the plot to determine if any garlic mustard is present and at what density. The PDA operator records the date of the visit, diameter of the population in meters, density of garlic mustard within the plot, number of dead stems present, and the method of treatment (Photo 10). Photo 10. A screen shot of the data collection page in our PDA garlic mustard program. For each plot, we list the date it was visited, the density and diameter of the population, how it was treated, and whether any dead stems were present. New points are created when unmapped populations of garlic mustard are discovered in the field during transecting. Prior to flagging and marking a new population with the PDA, it should be determined that the observed plants are not included within the diameter of a large, nearby population. If it seems that the plants are beyond the diameter of nearby populations and distinctly separated by native vegetation, it is safe to assume that it is a new population that should be flagged and mapped. If the potential new population is outside of the largest recorded diameter of a nearby plot, but not separated by native vegetation, one should consider widening the diameter of the already existing population. Edge flags are useful when the diameter of a plot is so wide that it is difficult to find the boundary with the naked eye. When these situations occur it is helpful to flag the north, south, east and west boundaries. Due to the fact that not all of the populations are perfect circles, we have considered the “diameter” to equal the longest axis of the population. Many populations are very linear in - 10 - shape, especially when infestations follow wildlife trails and established pathways, which means the recorded diameter—the longest axis—can be very large compared to the width of the population (Figure 3). The diameter of any plot varies from year to year and is adjusted by the Stewardship Crew if necessary. Longest Axis Figure 3. Diagram used to determine population diameter. The density of populations is also recorded in the PDA program and has been standardized to include three categories: “dense,” “scattered,” and “very scattered.” To be considered dense, 80-100% of the ground in the plot must be covered by garlic mustard, and the majority of garlic mustard plants touch each other on at least three sides. To be considered scattered, 21-79% of the ground is covered by garlic mustard, and the majority of plants touch two other plants. To be recorded as very scattered, 20% or less of the ground is covered by garlic mustard, and the majority of plants do not touch one another. These densities are shown in Photos 11, 12 and 13 respectively. Recording how many plants occupy a set amount of space gives us another criterion to evaluate the extent of the garlic mustard infestation on the LMR. We hope for plots to be very scattered instead of densely packed full of seed-producing plants. Photo 11. A garlic mustard plot classified as “dense.” Photo 12. A garlic mustard plot classified as “scattered.” Photo 13. A garlic mustard plot classified as “very scattered.” - 11 - Levee Road and Areas Outside of Management Units Populations of garlic mustard exist in areas that are not managed using the preceding protocol. This year, the Aldo Leopold Foundation worked with the neighboring Sand County Foundation (SCF) to chemically treat populations occurring along Levee Road from Schepp to County Highway T. During this treatment, two people wearing backpack sprayers walked down both sides of Levee Road and sprayed all garlic mustard within 10-15 feet of the roadside. Several areas within the Terbilcox property were also treated without transecting near the Van Hoosen house and along the trails south of the Legacy Center. These areas were sprayed using a 200-gallon gas-powered sprayer mounted on the back of the F-350 Super duty dually. Using low pressure, we sprayed as far into the properties as we could drag our 150-foot hose. No data about specific populations was collected for these areas and they were not flagged. Only work hours and gallons sprayed were recorded. Results Management of garlic mustard in 2013 began May 10 and ended July 10. Initial visits lasted from May 10 until June 14 and revisits started June 17 and were finished on July 10. There were a combined total of 39 work days this season; 24 during initial visits and 15 during revisits. However, the crew spent five hours or less in the field on 16 of the 39 work days. The timescale of treatment is displayed in Figure 4. Timescale of Inital Visits and Revisits Initial Visits Revisits 2013 Year 2011 2009 2007 2005 2003 100 04/10 04/10 120 04/30 04/3 140 05/20 160 06/09 Date (mm/dd) 180 06/29 200 07/19 220 08/08 Figure 4. Timescale of garlic mustard management at ALF from 2003 through 2013. - 12 - There are currently 2875 garlic mustard points recorded in the GIS system. 737 of these points are in the Potter Preserve, which is no longer managed using the protocol outlined in this report. 2012 of the remaining 2138 points were visited this year, which means 126 points were not found this year. New population identification numbers this year began with plot ID 2703. There were 120 new populations found, and the last plot ID assigned was 2817. This season the average plot size was 4.12 meters; the median size Plot Size Distribution (meters) was 3 meters; the maximum and 2% minimum sizes were 42 meters and 4% 14% 0m 0 meters respectively (Figure 5). Of 18% .01 - 1 m the 265 acres that we manage 15% 1.1 - 5.0 m garlic mustard on at the Aldo 5.1 - 10.0 m Leopold Foundation (excluding 10.1 - 15.0 m roadsides), garlic mustard plots 47% occupy 14.5 acres as of 2013 > 15.0 m (Figure 6). 92% of those 14.5 acres are characterized as having very Figure 5. Percentage of plots within each diameter size class. scattered densities of garlic mustard. Only 16 dead stems of garlic mustard plants were found this year, theoretically meaning that last year’s crew missed only 16 plants during treatment. During initial visits, chemical treatment occurred at 85% of all plots, no treatment occurred at 14% of all plots (usually because no garlic mustard plants were visible), and less than 1% of the plots were manually treated. Total Acres of Alliaria petiolata *Includes the Potter Property Until 2009 Number of Acres 20 15 10 5 5.4 0 0 2003 2004 8.0 11.0 10.4 11.2 12.4 14.2 13.3 14.5 2007 2008 2009 2010 2011 2012 2013 3.1 2005 2006 Year Figure 6. Total number of acres occupied by garlic mustard populations from 2003 to 2013. - 13 - During the 2013 season, the Stewardship Crew spent a total of 788 hours managing garlic mustard on the 15 core management units on the LMR (Table 1). We spent an additional 59.25 hours spraying SCF roadside and Terbilcox property with the gas-powered sprayer for a grand total of 847.25 hours of garlic mustard management. There were usually 3-5 crew members working in the field every day. This is the largest number of person hours spent on treatment in the history of garlic mustard management. During these hours, the crew sprayed 330 gallons of herbicide over the 25 days of initial visits. Additionally, ALF staff sprayed 420 gallons of herbicide with the gas-powered sprayer on unflagged roadside populations and areas on the Terbilcox property. Throughout the 15 days of revisits, the crew pulled and bagged 553 gallons of garlic mustard (Figure 7). Management Unit Initial Hours Revisit Hours Charlie’s Woods 53.5 73.25 Shack Pines 17.25 7 Orchard 4.5 1 Anniversary Unit 5.5 5 Shack Foundation 6 5 Sandhill 16.25 8.75 South of Birch Row 12.5 10 North Birch Row 30.5 15 Floodplain between Eddy Gordon and Levee 80.25 53 Lower Floodplain between Eddy Gordon and River Trail 119.5 64 Upper Floodplain between Eddy Gordon and River Trail 80 24.25 North of the River Trail 9 2.25 North of the Sands 9.25 4.5 West of Suevanna Clay Hill 9 39.5 6.75 15.75 492.5 295.5 Total Table 1. Hours spent in each management unit during initial visits and revisits in 2013. - 14 - Gallons of Bagged Alliaria petiolata and Gallons of Herbicide Sprayed 1200 Gallons 1000 800 600 Bagged Gallons 400 Gallons of Herbicide 200 0 2005 2006 2007 2008 2009 2010 2011 2012 2013 Year Figure 7. Yearly totals of gallons of bagged plants and gallons of herbicide used from 2003 to 2013. Discussion The ALF 2013 garlic mustard season proved to be just as extensive and strenuous as previous seasons, but in some different ways. This year, the crew adapted management in response to recommendations by last year’s interns and as a result of unforeseen record-breaking amounts of rain. New tools and strategies contributed to the effectiveness of our control methods and have resulted in some revisions to the garlic mustard treatment protocol. Overview of 2013 Garlic Mustard Season This year, the Stewardship Crew spent a record 788 person hours treating 14.5 acres of garlic mustard, which is the largest acreage of garlic mustard ever recorded on the Leopold Memorial Reserve. The crew also used 330 gallons of chemical in this treatment, which is a volume second only to the 2011 Stewardship crew’s highest number of gallons sprayed in a season. To account for these simultaneous expansions in time, resources, and area covered on the LMR treating garlic mustard, a number of factors unique to the 2013 season should be taken into consideration. Acreages of garlic mustard on the Reserve have both risen and fallen in the past few years of treatment. The 2011 Stewardship interns treated 14.2 acres of garlic mustard plots, more than any other Stewardship Crew in the project’s history. In 2012, this number fell to 13.3 acres, which marked the first decrease in treated acres of garlic mustard since 2008. The 2012 Stewardship Crew hoped to attribute this decline to the Aldo Leopold Foundation’s long term - 15 - treatment program. However, this year’s data suggests that weather probably played a major role in the flux of the past couple years of garlic mustard populations. Local news sources cite June 2013 as the fourth wettest June in 145 years of records taken by the National Weather Service. The Cap Times also labels 2013 as “the anti-2012” in terms of temperatures and precipitation (Milewski 2013). These observations extend to the Aldo Leopold Foundation. Last year, drought affected most plant communities in southern Wisconsin. The growing season started earlier than normal, causing the 2012 Stewardship Crew to start treating garlic mustard on April 22, almost a month earlier than the 2011 and 2013 Crews, who started on May 11 and 10, respectively. Lack of rain and high temperatures likely contributed to the fewer acres of garlic mustard treated in 2012. Second year intern Leann Wolthusen reports observations of heat stress in populations of garlic mustard by the end of initial visits last year, which concluded on May 24. She also notes that a fungus was present in many populations and probably further stressed the plants, causing below-average growth and seed production. Even native prairie plants produced fewer flowers and seeds in response to severe temperatures, with some even failing to reach normal heights. This year, we postulate that high levels of rain and cooler temperatures brought garlic mustard back in full force, or perhaps even more vigorously than in “average” years. We have observed that almost all vegetation is considerably more lush this year than the past few years, despite the long winter that postponed spring until the very end of April. It is worth proposing that more garlic mustard seeds than average might have germinated this year as a result of the favorable conditions in 2013, especially following last year’s particularly unfavorable weather. In addition, plenty of rain throughout April and June might have given the plants enough resources to flower and produce seeds longer than usual. As of August 21, we continue to see the occasional garlic mustard plant in flower. The reaction of plants to higher levels of rain and cooler weather in 2013 likely contributed to the increase in treated acreage of garlic mustard on the Leopold Memorial Reserve. An interesting occurrence that is likely also a consequence of wet conditions are observations of garlic mustard plants that we lightheartedly label as “zombie GM.” These are plants that have been sprayed with glyphosate solution during initial visits, appear to have been affected to an extent by the chemical, but still remain vigorous enough to continue to flower and produce seed pods. They usually have a purple or yellow color and may droop or look withered. The majority of these plants were found in areas that 1) we sprayed, and it rained within an hour or close to an hour after spraying, or 2) we sprayed in particularly heavy dew. - 16 - As mentioned in the Methods and Materials section, our 2% glyphosate solution must dry for a minimum of one hour to effectively kill the plant. In the event that rain occurred within an hour after spraying, the Stewardship Crew made sure to revisit and re-spray the area to ensure the effectiveness of the chemical. However, we may want to further examine the effectiveness of the chemical in heavy dew and consider extending the required drying period to 90 minutes. A 90-minute dry period might extend the work of Stewardship interns during initial visits due to a greater possibility of having to re-spray after rain, but it would also cut time during second visits since the crew would not need to hand pull entire populations of zombie garlic mustard. Another option may be to start an hour later in dryer conditions than have to re-spray populations or risk the seed out of plants that survive chemical application as a result of dilution by rain or dew. Another consideration to ensure that all plants are killed completely is correct spraying technique. Seventy percent of the plant or more should be covered with chemical after an adequate application; and the addition of the Tracer red foam dye to the glyphosate mixture should make the covered areas easy to see. It’s better for the plant to be dripping with chemical than too dry. However, spraying only the top of the plant until it drips doesn’t count— the entire length of the plant must be covered to make sure that the entire plant dies, not just the top or sprayed section. This is important because parts of the plant can survive and resprout during the growing season to flower and produce seed. Previous Stewardship Crews have conducted some informal experiments to test the viability of seeds produced by plants that have been sprayed with glyphosate. In most years, initial visits continue into the time that Alliaria petiolata starts to produce siliques and form seeds. To understand whether these seeds are affected by chemicals, the Stewardship interns prepared the seeds from these plants for germination by subjecting them to 100 days of cold stratification (refrigeration). This is the required pre-treatment to prompt garlic mustard seeds out of dormancy. None of the seeds in these experiments successfully germinated, suggesting that treatment with glyphosate sterilizes seeds and prevents regeneration. However, we are unsure whether seeds react the same way from a plant that has been only partially or inadequately sprayed with chemical, or from a plant that has been rained on after chemical application. In any case, this information should be noted only as a safeguard against the occasional plant that may release seeds even after full chemical treatment. All staff, interns, and volunteers should be trained in correct spraying technique from year to year and monitored throughout the field season. Because the weather seems to have given garlic mustard a leg up this season, significant additions to the Stewardship Crew’s average workday and the overall length of the 2013 treatment season were necessary. Of the 42 possible work days during the season, three full - 17 - rain days kept the Crew completely inside, and an additional 16 days were cut short by rain. This brings a total of 45% of our work days to have been affected by rain. To make up for lost time, our crew normally worked 10 hour days, starting at 7 o’clock in the morning and ending at 5 o’clock in the evening. Even with this extension of daily hours, progress in the field was often slow. In addition to the normal year-to-year obstacles of dense vegetation and exposure to heat, humidity, and insects, the 2013 Crew also dealt with higher water levels in the floodplain and an explosive mosquito population. Whether we forded sloughs or decided to divide units into chunks to avoid water crossings, transecting this year took extra time. Water in the sloughs was often too deep to walk across, even in tall rubber boots (Photo 14). The time we took to walk back to a suitable crossing at the end of each transect quickly added up, with no sprayed garlic mustard to show for this added time. Photo 14. Stewardship intern Steven Bachleda transects through high water in the floodplain forest. On the other hand, if we decided to split units into chunks between sloughs to avoid crossing the water, the trail and Kubota were inevitably inaccessible from a particular chunk. This caused problems because the Kubota held our supply of chemical, our drinking water, and bug spray; so if we needed to refill our packs or grab a drink (important in the summer and especially because we wear full jumpsuits to protect us from chemical spills), the walk back to the Kubota usually took at least 5-10 minutes, again adding up as lost time. This was the first year that the Stewardship Crew needed to regularly extend work days to finish treating all management units by mid-July, when most garlic mustard starts to release seed in earnest. Simultaneous treatment of Japanese hedge parsley might also have contributed to our extended work days, although probably not as much as rain. Normally, Stewardship interns treat garlic mustard from May through July and subsequently treat Japanese hedge parsley from the end of July through the first week or two of August. This year, the Stewardship Crew treated some Japanese hedge parsley at the same time as garlic mustard, especially when we transected through units that house known populations of both invasives. Although the focus during this time stayed on garlic mustard, the crew still took time to look for Japanese hedge parsley flags, find and treat populations at the flags, and treat large unflagged populations on roadsides and edge habitats, which are the species’ preferred growing conditions. More information about Japanese hedge parsley can be found in the Aldo Leopold Foundation - 18 - Stewardship Crew’s Japanese hedge parsley report, on our website at www.aldoleopold.org/WoodlandSchool/resources.shtml. The unexpected long work days were understandably stressful for interns at times. In future years, we will warn potential summer interns that impromptu overtime work is a real possibility, though it often depends solely on the weather and how fast we can progress through our projects. Experimentation of some new equipment that may be useful in wet years and thorough testing of current equipment has been suggested after working through this field season. A few unpleasant days were spent wading through sloughs in leaky boots or in boots that weren’t tall enough to keep water out. Hip waders have been suggested for trial by next year’s interns, and we will continue to brainstorm for other water-friendly, time-saving PPE. Changes in protocol regarding maps and mapping The 2013 Stewardship Crew implemented most changes in protocol recommended by the 2012 Stewardship Crew. Most of these changes improved the garlic mustard project and are recommended for continuation next year. The most important recommendation for next year’s Crew is to have each crew member continue to carry a paper map while transecting. Prior to 2012, the only crew member to carry a map of the work unit was the person collecting data with the PDA. However, maps available in the ArcPad GIS program are limited in detail and illustration of progress. Although ArcPad shows each garlic mustard point in a management unit, the PDA maps fail to indicate at a glance which points have been treated and which have not; fail to clearly show all points unless adequately zoomed in; fail to show the details of the landscape in each unit (e.g. thickness of vegetation and sloughs), and the PDA allows no space for field notes. Last year, one person on the crew collected data with the PDA and one person carried a paper ArcGIS map to mark off which points had been completed. This system made it easy for the 2012 crew to see points that they missed and go back to treat them. This year, the Stewardship Crew expanded the 2012 crew’s use of paper maps. At least two people on the 2013 crew carried paper maps in addition to the person collecting data on the PDA. These maps were useful not only in showing which points that we may have missed; but also helped us see which points are incorrectly placed on the maps; helped us strategize our transecting by showing where potential sloughs and tough spots might be; allowed us to better track our progress and show our position in each unit; allowed us to reconcile our data when the PDA stopped working; and gave us a space to take notes on how long we spent on each unit, where we may have had to re-spray because of rain, which new population number we used last, and suggestions for next time (Photo 17). Having two people carry a map further strengthened our - 19 - data and allows us to cross examine independent notes. We strongly suggest that the 2014 Stewardship Crew continue to carry paper maps in addition to the PDA. Photo 15. A paper map in comparison to the map shown by the PDA garlic mustard program. The paper maps allowed us to mark off completed points, keep track of work hours, and keep other notes in a way not possible on the PDA. Next year’s crew should also expand the use of paper maps by knowing how to move points from an incorrect location to the correct location in ArcGIS. Some points shown on the maps to be in one unit were actually found in adjacent management units. These placement errors were particularly frustrating after spending time searching for the plots in one management unit, deciding that the flag was missing, creating a new flag and placing it in the “correct” location as shown by the GPS, and then finding the flag the next day in the next management unit. All efforts should be made to correct the placement of misplaced flags, and crew members should make sure to manually place new population locations on the ArcPad map if the Bluetooth GIS unit isn’t working at that time. Notes taken on the 2013 paper maps and entered into our Excel data file will alert the 2014 crew of flags that may have been incorrectly placed. In addition to misplaced plots, paper maps also illuminated the number of duplicate garlic mustard points existing in the management units. When the ALF Stewardship Department included Potter Preserve in their plans for garlic mustard control, some of the same flag numbers were assigned to plots in both the Leopold Memorial Reserve and Potter Preserve. After selling Potter Preserve, these duplicate points were not deleted, causing some points to show two different locations in the PDA. Although it’s not hard to figure out which of these duplicate points is located on the Leopold Memorial Reserve, removing the duplicate from the garlic mustard GIS file would clean up unnecessary, redundant data and streamline the data collection process. - 20 - Of greater concern than duplicate points shared by Potter Preserve and the LMR are duplicate flag numbers within or between the management units on the Leopold Memorial Reserve itself. We came across a number of these and were unable to record data for both points, since only one point would come up on the PDA for that particular number. We marked data for these duplicate points on our paper maps and noted the duplicate numbers in our Excel data files. These points should be renumbered as soon as possible for accurate data collection. Analysis of new equipment The 2013 Stewardship Crew still has minor deliberations on some new equipment that we tested out this season. Instead of using 33-gallon contractor garbage bags to carry our handpulled garlic mustard through the field during revisits, we purchased six dry bags with overshoulder straps to make transporting the plants easier. The bags were especially useful through prickly ash and other thorny species that catch and rip the plastic garbage bags. For the most part, the dry bags worked well. However, since they have a considerably smaller volume than a 33-gallon bag, the dry bags required emptying between transects into garbage bags on the Kubota. This was usually no problem, but once in a while a transect would yield more than the dry bag’s capacity of garlic mustard. We made do by carrying our extra plants in bundles or by using empty space in somebody else’s bag. In only one case did we abandon the dry bags in favor of taking garbage bags into the field—we recognized the area as one that was washed out by rain a little more than hour after spraying, and speculated that the chemical did not dry in time to be effective. We also purchased miniature clip boards to make writing on maps and flags easier in the field. Full size clip boards are too large and cumbersome to carry, especially when each crew member carries at a minimum a map, a marker, a quiver of flags, and a dry bag or backpack sprayer. The miniature clip boards are half the size of normal boards and are small enough to attach to a string around your neck or wrist. They free up hands when you clip the paper map to the board and tie the board to yourself. However, these small boards still got in the way sometimes and some crew members felt them not to be worth the trouble of carrying around. Suggestions and potential changes in protocol for 2014 The most important thought in potential changes in procedure for the 2014 interns is an examination of the most effective number of people working on garlic mustard in the field. In 2012, initial visits were conducted by Leann Wolthusen and Benjamin Van Thiel, the two ninemonth Stewardship interns that year. Two six-week interns joined them for revisits and two sixweek interns joined the crew after the garlic mustard project was finished completely. Predictably, garlic mustard revisits went faster with four crew members in 2012 than with only two people working on initial visits. The 2013 Stewardship crew predicted that more people in the field would make the project go even faster. We were excited to have three six-week interns on board for the entire garlic mustard season, totaling a crew of six people. - 21 - As we worked through the season, each person in the crew agreed that the most effective number of people working together in the field is three to four. Even though six people transecting at the same time covers a lot of ground, other factors slowed us down so that our person-hours exploded, but the overall time we took to cover specific units kept us on par with a two-person crew. Our downfall was the amount of time lost waiting for other crew members to “catch up.” Whether one half of the crew had to walk through dense prickly ash while the other half strolled through a meadow, or five crew members hit garlic mustard plots at the same time and had to queue up to tell their information to the data collector, we were always waiting for someone. Although no fault of any specific person, to stay together and make decent progress with a six-person crew was almost impossible. One advantage to having so many people on hand to work on garlic mustard was the ability to split people off to lead volunteers, go on field trips, or take vacations. One or two people at a time would receive a needed break from the physical and mental stresses of the garlic mustard project while the rest of the crew continued transecting. On these days, with a garlic mustard crew of three or four people, we noticed a marked improvement in our progress through management units. In a smaller group, it is much easier for crew members to keep pace with one another, much easier for the data collector to keep up with the number of plots encountered by fewer people at a time, and much easier to organize and encourage a group of three or four than of five and six. Taking breaks from transecting for garlic mustard is a luxury rarely experienced by Stewardship Crews prior to 2013. Two full months of treating garlic mustard is arguably more mentally taxing than physically taxing. In previous years, Stewardship interns have aimed to take one day a week off from this project to avoid burn-out. This year, the six crew members available to work on garlic mustard throughout the entire season allowed all three nine-month interns to take week-long vacations and come back feeling refreshed. For this reason, we suggest that future nine-month interns still consider hiring six-week interns during the same time period (instead of having one intern work six weeks, and then a second intern the six weeks after that). However, instead of attempting to work as one big group, the Stewardship Crew should experiment with working in teams of three or four people. Teams of interns would be beneficial in that they may “divide and conquer” management units. Two teams would be able to tackle two small units at the same time or one large unit by starting at opposite ends and meeting in the middle. However, some obstacles stand in the way of team garlic mustard treatment. Although we have two utility vehicles available for our use, we only have one 35-gallon chemical tank that loads onto to the Kubota to refill our backpack sprayers. Two teams would need to come up with an effective way to refill, which we did often - 22 - during the day. This might mean that one team would have extra chemical in packs in their vehicle that they would switch out when necessary or that the two teams would rendezvous and refill at some point during the day. Communication between the two teams would also be essential; this might be achieved with radios. Despite these and other possible kinks that need to be worked out for this strategy of garlic mustard treatment, future crews should sincerely consider the two-team system as a possible way to speed up the garlic mustard season as a whole. Other changes in protocol suggested by last year’s crew and found to be successful in this year’s garlic mustard treatment included eating lunch in the field, physically attaching equipment to your person to avoid losing gear in the field, and maintaining early start times. Although sometimes hot and buggy, eating lunch in the field saved time from driving the 15-20 minutes back and forth from the Legacy Center. We also found that our crew of six people managed to lose a variety of markers, stylus pens, and maps in the dense vegetation on the Reserve. Although small, these pieces of equipment can be relatively expensive and potentially debilitating when lost. Attaching bright flagging tape to these items and tying them to the jumpsuits was very effecting in keeping them available for use. Early start times were essential last year and this year because of the heat in 2012 and because of the rain in 2013. We recommend that the 2014 crew plan to start early during garlic mustard season. If they find that an early start is unnecessary in the coming year, they can adjust the timing accordingly; but if they do require an early start for one reason or another, they will be mentally prepared. Notes on Management Units These notes break down suggestions for treatment protocol by unit. What may have worked this year may not work the same way in coming years, but some institutional knowledge may be beneficial to future interns. Charlie’s Woods: The season starts with undoubtedly the toughest management unit. A recent timber harvest has allowed for the establishment, spread and proliferation of Rubus spp. Transects are long and thorny with raspberry and blackberry canes over 6 feet tall. We divided the unit into north and south units and tackled one at a time, running east and west transects. Sand County Foundation land borders the south edge of the unit and populations of garlic mustard are dense and boundaries are not marked. Go only as far south as the southernmost flag. Past the farthest flags, the south and southeast sides of Charlie’s Woods serve as a buffer zone between Sand County Foundation Land and Aldo Leopold Foundation Land. Although hard to ignore, you won’t be able to spray and pull all of the garlic mustard in these areas. East of the main trail into Charlie’s Woods, we didn’t transect and went point to point instead. - 23 - Shack Pines: Enjoy this leisurely unit with the call of the black-billed cuckoo echoing in the background. Concentrations of garlic mustard are highest at the edge of the pines bordering the Shack prairie. North and south transects work great. South Birch Row: North and south transects between the Birch Row Trail and the Shack prairie are recommended. On the western finger of the unit, run a single east-west transect. Japanese hedge-parsley is abundant and should be sprayed during initial garlic mustard visits. North Birch Row: North and south transects work best. The Birch Row Trail is the divider between North and South Birch Row. Existing alongside garlic mustard are honeysuckle, Japanese hedge parsley, barberry, and multi-flora rose. Spray these invasives as you find them. Anniversary: A small unit with large populations. Be thorough while transecting here as plants can be easily over looked. It’s a small unit but it will take more time than anticipated. Sandhill: This one is fun! Look for spring ephemerals, morels and snakes during initial visits. Run east and west transects from the Good Oak Trail to the Shack Pines. On the western edge of the unit (the hill) run north and south transects until the Good Oak Trail is reached. Orchard: There are only a handful of flags in this unit but plants are abundant. Double check the western boundary bordering Sandhill where garlic mustard is most concentrated. Shack Foundation: Look for morels under the meat rock! This unit goes fairly quick and even quicker when there is water covering the floodplain. North and south transects work best, and be careful on the northern boundary – a slippery slope. There are points at the bottom of the slope so you do have to walk down it. Floodplain North of the Sands: Transects are awkward in this unit. The best method to go about transecting is still to be determined. Vegetation is thick along the river and there are flags hidden throughout. Be prepared for a slower pace and narrow transects. Take a break and enjoy the view of the Wisconsin River or jump in to cool off. Floodplain between Eddy Gordon and Levee: The going is about to get tough; be prepared for a long couple of days. This unit is a good primer for the following unit. Run north and south transects between Levee Road and the Eddy Gordon Trail. Work around the water to the best of your abilities. Wide transects can be used in the most of the western portion of the unit (Suevanna) to find the handful of flags that are there. The game of who can find the most deer skulls also begins here (our skull family keeps growing!). Lower Floodplain between Eddy Gordon and River Trail: This unit will take the most time compared to all other units. We recommend starting at the western boundary and working east, running north and south transects. The northern boundary for this unit is not well defined; - 24 - it is a slough, more evident in certain areas than others. The western boundary of the unit should be thoroughly transected because it borders the unmanaged Sand County Foundation land. Several new, large populations were found in 2013. Work around the water as best you can, and park the Kubota on the Eddy Gordon Trail. Don’t let the turkeys spook you when you jump them. Endure! Upper Floodplain between Eddy Gordon and River Trail: You have made it to the home stretch. This unit is the least troublesome of all the floodplain units. Again, north and south transects work best. Once you’ve worked through the buckthorn and prickly-ash thickets, your pace will quicken. North of the River Trail: There are a handful of points in this unit; however, it covers a large area. Crews have typically gone point to point if rushed for time. It is best if the unit is transected so that crew members can scout for new populations. North and south transects work best. Morel Madness at a few of the points! West of Suevanna and Trail Intersection (Hell’s Corner): This unit is difficult and will leave you feeling depleted. Do the best you can here as this unit serves as a buffer between ALF and SCF. You can spend days spraying in here, so put a limit on the gallons you will spray at 20. Conquer the 1-acre plot! Clay Hill: The end is near! The 2013 crew ranked this unit as our second least favorite, just behind Charlie’s Woods. Run north and south transects from Levee Road to Chapman Lake. There are numerous populations in this unit that are 10 meters or larger. No new populations were created in 2013 because the crew determined the existing plots covered the entire area of the unit. Dogwood thickets, buckthorn slash and fallen trees make the southern portion of this unit difficult to transect. However, it can be done! - 25 - Appendix A Raw Data Table 2. Chemical Control (Initial Visits) of Garlic Mustard by ALF Land Stewardship crew and volunteers, LMR, Spring 2013 Gallons of Total # 2% Date # of People Location Person Hours Glyphosate 5/10 3 7 1 Started Charlie’s Woods 5/13 3 16.5 3.25 Charlie's Woods 5/14 3 21 4 Charlie’s Woods 5/15 3 19.5 5 Finished Charlie's Woods and started Shack Pines Unit 5/16 5 15 3.5 Finished Shack Pines Unit and started South Birch Row Unit 5/20 5 19.5 9.5 Finished South Birch Row, Anniversary Unit, and Foundation Unit; started Sand Hill Unit 5/21 5 23.25 15 Finished Sand Hill Unit and Orchard Unit; started North Birch Row 5/22 4 5 2.75 North Birch Row 5/23 4 23 9.25 Finished North Birch Row 5/24 4 21 18.5 Floodplain between Eddy Gordon and Levee 5/28 4 8.25 9.25 Floodplain between Eddy Gordon and Levee 5/29 4 28 21 Floodplain between EG and Levee 5/30 3 10.25 8.75 Floodplain between EG and Levee 5/31 3 21.75 14.5 Finished Floodplain between EG and Levee, Suevanna, and North of the River Trail 6/2 3 15.5 15 Lower Floodplain between EG and River Trail 6/3 5 32.5 9.75 Lower Floodplain between EG and the River Trail 6/4 5 21.5 18.5 Lower Floodplain between EG and River 6/5 6 6 5 Lower Floodplain between EG and River - 26 - 6/6 5 27 29 Lower Floodplain between EG and River 6/7 5 17 14 Lower Floodplain between EG and River 6/10 7 32.25 10.5 Upper Floodplain between EG and River; Sand County Foundation Roadside 6/11 6 48 22.25 6/12 5 5 0 Upper Floodplain between EG and River; Sand County Foundation Roadside Upper Floodplain between EG and River Trail 6/13 6 41.75 37.25 Finished Upper Floodplain between EG and River Trail, North of the Sands, and Hell’s Corner; continued SCF Roadside; started Clay Hill 6/14 7 43 42.5 Finished Clay Hill and Shack Triangle 6/17 2 14 125 SCF Roadside/Terbilcox Property (with dually) 6/18 2 11 70 SCF Roadside/Terbilcox Property (with dually) N/A 1 N/A 226 Terbilcox Property between Levee and Van Hoosen Roads (with dually) Totals 2-7 553.5 750 Table 3. Revisiting Garlic Mustard Populations by ALF staff, LMR, 2013. Date # of Total # Person Gallons Location People Hours Pulled* 6/17 4 30.75 N/A Started Charlie's Woods 6/18 3 21.5 30 Charlie’s Woods 6/19 4 21 25 Finished Charlie’s Woods and Shack pines 6/20 4 30 12.85 Finished Anniversary Unit, North Birch Row, and South Birch Row 6/21 4 14.75 12 Finished Orchard, Foundation, and Sandhill units 6/24 3 23 20 Floodplain from Levee to Eddy 6/25 4 24 10 Floodplain from Levee to Eddy 6/26 4 30 19 Finished Floodplain from Levee to Eddy and Suevanna; started Lower Floodplain between Eddy Gordon and River Trails 6/27 3 9.5 15 Lower Floodplain between EG and River 7/1 3 21 33 Lower Floodplain between EG and River 7/2 3 20.25 213 Lower Floodplain between EG and River 7/3 3 18.75 36 Finished Lower Floodplain between EG and River; - 27 - started Upper Floodplain between EG and River North of the Sands Hell’s Corner North of the River Trail; Clay Hill 7/5 3 4.5 0 7/9 3 6.75 75 7/10 3 18 35 Total 3-4 293.75 535.85 * The gallons of garlic mustard hand-pulled included both plants that were missed during initial visits and any plant that was treated but did not appear dead, or had seeds/siliques that were looked as though they may still be viable. Table 4. Total garlic mustard removal by ALF staff and volunteers, 2003-2013. 2003 2004 2005 2006 2007 2008 2009 2010 2011 2012 2013 * Hours 189 275 196 184.5 361 439.75 496.25 508.25 621.25 460.1 847.25 Spray 31 221 128 183.5 243 133.25 206 208.75 348 127.15 524 (Gallons *** Used) Gallons 2970** 1584 1134 565 565.5 156.75 531.3 810 554 460.5 535.85 Bagged New No 499 180 331 157 85 286 192 301 94 120 Populations data Found * 2004 totals for LMR included efforts made at Potter Preserve ** In 2003, interns chose to pull adult plants at the majority of sites rather than treat with herbicide. *** 2012 interns did not chemically treat the Clay Hill unit ****2013 hours include SCF roadside spraying and gas-powered spraying on Terbilcox property. Also, the 2013 Stewardship Crew consisted of 3-7 people instead of 2-6 in other years. Table 5. Number of garlic mustard populations and total acres. 2009 2010 2011 Year # of Plots Acres # Plots w/ Dead Stems 2012 2013 1547 11.23 1749 12.43 2040 14.15 2134 2258 10.2 14.5 123 95 121 85 13 - 28 - Works Cited Blossey, B., V. Nuzzo, H. Hinz, and E. Gerber. 2001. Developing biological control of Alliaria petiolata (M. Bieb.) Cavara and Grande (garlic mustard). Natural Areas Journal 21:357-367. Blossey, B., V. Nuzzo, H. L. Hinz, and E. Gerber. 2002. Garlic mustard. Pages 365-372 in R. van Driesche, B. Blossey, M. Hoddle, S. Lyon, and R. Reardon, editors. Biological Control of Invasive Plants in the Eastern United States. Forest Health Technology Enterprise Team, Morgantown, West Virginia. “Garlic musard (Alliaria petiolata).” Invasive Species. Wisconsin Department of Natural Resources. 5 June 2013. Web. 27 August 2013. Milewski, Todd D. “2013 brings an about-face in Madison's weather; June was 4th wettest month on record.” The Cap Times. 1 July 2013. Web. 1 September 2013. Munger, Gregory T. 2001. Alliaria petiolata. In: Fire Effects Information System, [Online]. U.S. Department of Agriculture, Forest Service, Rocky Mountain Research Station, Fire Sciences Laboratory (Producer). Available: http://www.fs.fed.us/database/feis/ [2013, July 16]. Razor Pro Specimen Label. Nufarm Limited. 2013. Web. 24 September 2013. Nuzzo, Victoria. 2000. Element Stewardship Abstract for Allaria petiolata (garlic mustard). Nature Conservancy. Arlington, Virginia. “Species pages: Garlic mustard.” The Virtual Nature Trail at Penn State New Kensington. The Pennsylvania State University. 5 January 2006. Web. 27 August 2013. - 29 -