Roche Diagnostics VENTANA product portfolio

Transcription

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Roche Diagnostics International Ltd.
CH-6343 Rotkreuz
Switzerland
www.roche.com
www.ventana.com
Roche Diagnostics
VENTANA product portfolio
© 2011 Ventana Medical Systems, Inc.
and Roche Diagnostics International Ltd.
VENTANA, the VENTANA logo, BENCHMARK,
CONFIRM, DISCOVERY, INFORM, NexES, OPTISURE,
PATHWAY, SYMPHONY, ultraView and VANTAGE are
trademarks of Roche.
All other trademarks are the properties
of their respective owners.
E2123 1011A
06562604001
third edition October, 2011
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Roche Diagnostics VENTANA Product Portfolio | Third Edition
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Intelligent Systems |
Intelligent Diagnostics |
Intelligent Results |
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We innovate staining platforms and workflow solutions that
enable you to handle patient samples individually, even at
your highest volumes. You can count on the people who created
the first fully automated IHC, ISH and FISH slide staining
capabilities to deliver the highest level of sophistication and
consistency to your lab.
Guided by science, we have developed the industry’s largest,
most advanced portfolio of pre-diluted, ready-to-use reagents
to support your lab. All of our antibodies are specially
optimised for use on our cutting-edge instrumentation, to
maximise the efficiency, precision and quality of routine and
complex stains.
Our Patient Focused Solution is an Intelligent System that gives you
total confidence in your ability to deliver the right patient result.
www.ventana.com | www.roche.com i
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Now available | The lab of the future.
Experience the ease and confidence that comes with optimised, ready-touse reagents, fully integrated automated staining platforms and worldclass workflow—all from a strategic partner who is positioned to deliver
the most precise and personal cancer diagnostic solutions to any lab,
anywhere in the world, right now.
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Table of Contents
Workflow Solutions 1
VENTANA VANTAGE Workflow Solution
3
Workflow Consulting
7
H&E 11
VENTANA SYMPHONY Fully Automated H&E System
13
Special Stains 17
VENTANA NexES Special Stains System
19
Special Stains
22
Ancillary Reagents
33
IHC & ISH 35
VENTANA BenchMark ULTRA System
37
VENTANA BenchMark XT System
41
VENTANA BenchMark GX System
45
Detection Chemistries
49
Primary Antibodies by disease state
55
Primary Antibodies by alphabetical listing
68
Immunofluorescence Antibodies
179
Research Primary Antibodies
183
Molecular Probes
185
Ancillary Reagents
193
VENTANA Digital Pathology 211
iScan Coreo Au Slide Scanner
213
Virtuoso Image Management Software
217
Customer Services 221
Technical Consultation Centre
223
Education and Training
227
Index 233
Disclaimer
While we have made every effort to provide accurate and complete information
in this catalogue, various data may change or be updated by the company at
any time without notice. The company provides no warranty, express or implied,
as to the accuracy, reliability or completeness of furnished data. Photos are an
indication only and do not guarantee staining results.
Some products may not be available in your country. Check with your local Roche
representative for the availability of products in your region and the applicable
General Terms & Conditions.
www.ventana.com | www.roche.com iii
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iv Smart System. Smart Diagnostics. Smart Results. Powering cancer intelligence.
800.227.2155
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WORKFLOW SOLUTIONS

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Workflow Solutions |
VENTANA VANTAGE Workflow Solution 3
Workflow Consulting 7
www.ventana.com | www.roche.com 1
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VENTANA VANTAGE Workflow Solution | WORKFLOW SOLUTIONS

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VENTANA VANTAGE
Workflow Solution
The VENTANA VANTAGE workflow solution is the first of its kind in
the industry. Finally, labs can enjoy complete chain of custody, lean
integrated processes and full visibility into lab operations any time, from
anywhere. Our complete solution for histology labs—hardware, software
and Workflow Consulting—offers a commanding view of your complex
operation from a single strategic perspective. It is an end-to-end product
that automates, streamlines and integrates lab work as well as information
flow to provide maximum productivity and to mitigate risks.
The VENTANA VANTAGE workflow solution is designed using Lean Six
Sigma principles and includes expert Workflow Consulting support to help
you obtain immediate and ongoing workflow benefits.
What’s on YOUR mind?
I need real-time insight to manage my workflow.
• See a comprehensive dashboard of lab performance at
any time
• Identify opportunities for improvement in quality, staffing
and efficiency
• Facilitate compliance and audit reporting at a
moment’s notice
I need fast turnaround times and workflow efficiencies.
• Partner with lean histology experts to improve
your workflow
• Introduce lean single piece flow through
intuitive touchscreens
• Simplify workflow steps, minimise the need for paper
and remove non value-added activities
I need to eliminate errors and mitigate risk.
• Obtain positive specimen identification for all
cassettes, blocks and slides, with barcode scanners at
each workstation
• Immediately locate any patient’s slide, at any point in the
process, from any place with internet access
• Mitigate errors with “one label, one time”
slide identification
WORKFLOW SOLUTIONS | VENTANA VANTAGE Workflow Solution

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Workflow
Your integrated workflow management solution
Enterprise class software delivers workstation-specific
tools to ergonomic touchscreens and integrates with your
VENTANA instruments. As your lab processes specimens
with the VENTANA VANTAGE workflow solution, sample
integrity is verified, comprehensive workflow information is
captured and meaningful performance analysis is delivered
via dashboards, reports and more.
The VENTANA VANTAGE workflow solution is turnkey,
can often be implemented rapidly and integrates with the
leading Laboratory Information Systems (LIS). Take the
stress out of managing your lab’s workflow. With VENTANA
VANTAGE, you can focus on the value-added activities that
help make your lab successful.
Establish complete chain of custody
The VENTANA VANTAGE workflow solution brings all of our automated platforms together, creating a complete, unbroken
chain of custody that encompasses your entire lab.
Locate any specimen, block or slide immediately
Ask VENTANA VANTAGE to locate any patient’s slide, on any instrument, at any point in your process—and count on
immediate, accurate results.
Eliminate redundancies, reduce errors
Eliminate data re-entry, relabelling and labelling errors with “one label, one time” technology and barcode scanners at
every workstation.
Populate patient details accurately
Retrieve patient details with a quick barcode scan, expediting review and reducing opportunities for errors.
Ordering Information
4 Product
Part Number
Description
VENTANA VANTAGE Workflow Solution
For further
information,
contact local sales
representative
The VENTANA VANTAGE workflow solution suite is designed to
improve productivity and mitigate risks in anatomical pathology.
This solution incorporates Lean Six Sigma principles and provides
comprehensive specimen tracking, greater quality control and
unparalleled informatics.
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VENTANA VANTAGE Workflow Solution | WORKFLOW SOLUTIONS

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Better together |
VENTANA VANTAGE Workflow Consulting adds the power of lean
expertise, industry experience and personal attention to the achievement
of your laboratory’s goals.
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Workflow Consulting | WORKFLOW SOLUTIONS

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Workflow Consulting
Workflow Consulting empowers today’s highly complex labs to simplify,
streamline and succeed. Your partner in Lean automation for years, we are
expanding our hands-on services to help you maximise your results and
lower your costs.
Lean Six Sigma: These are big buzz words in today’s top-performing
industries. But the best, most powerful tools in the world are worthless
until we learn to use them in our everyday lives. What do the words really
mean and how will they help you run your lab at maximum efficiency
every day? Our workflow consultants will help you understand and apply
Lean principles to address the real-world issues you face in your lab.
I need fast turnaround times and worflow efficiency.
• Standardise your processes to ensure consistent
quality outcomes
• Optimise your workflow to reduce sample transport time,
wait time and waste
• Identify where adding VENTANA products can eliminate
non-value-added activities
I need to eliminate errors and minimise risk.
• Locate potential sources of mislabelling and cross
contamination in your lab’s workflow
• Suggest improvements to accurately track rework and
reduce its occurrence
• Share best practices and lessons learned
I need to improve performance without increasing cost.
• Recommend staffing model adjustments to balance your
workflow and reduce overtime
• Review layout designs that maximise the use of space at
your facility
WORKFLOW SOLUTIONS | Workflow Consulting

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Workflow Consulting
Our consultants are trained experts in Lean Six Sigma—and they are your biggest advocates as prior lab staff themselves.
Our team brings you:
Experience
Have a tough challenge? We have probably tackled one like it before. Our team has completed more than 125 anatomical
pathology lab projects to date all around the world. A typical Workflow Consulting engagement is five days, during which
the consultant observes and documents Lean workflow opportunities, compiles findings and shares detailed improvement
recommendations with the lab’s leadership and staff.
Proven success
Our track record for success is proven in many labs worldwide. In fact, one recent customer reports a drop in slide
identification errors—from 1.87% slide errors to .00003%—after a workflow assessment and VENTANA VANTAGE
installation.
Cutting-edge practices
We are focused on integrating new technologies, IT systems and hardware with the most efficient workflow to help you
reach optimal performance.
Partnership
Our consultants are your partners in long-term lab improvement, so if you have questions three weeks or three months
after the project is complete, we are at your service.
Accessioning to archiving
Our workflow consultants will partner with you to make your lab’s process easy, fast and reliable. When we look at your
lab, we see the big picture. Working closely with your team, we will identify and address the opportunities to optimise
your workflow. Optimise your lab’s performance from the moment you receive a sample to the time you deliver a valued
patient’s response.
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Workflow Consulting | WORKFLOW SOLUTIONS

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Better together |
Partnered with VENTANA VANTAGE, our total workflow solution
(hardware, software and consulting) provides real-time information and
reports to help you see how and where you are improving.
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H&E

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H&E |
VENTANA SYMPHONY Fully Automated H&E System 13
Ventana part number in black, Roche item number in blue.
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VENTANA SYMPHONY Fully Automated H&E System | H&E

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VENTANA SYMPHONY
Fully Automated H&E System
The VENTANA SYMPHONY automated H&E slide staining platform
offers individual slide staining with fresh reagent application for every
test, protecting against tissue cross-contamination that occurs in “dipand-dunk” staining baths. Specially designed to enhance lab efficiency,
to improve staining quality and to mitigate risks, the VENTANA
SYMPHONY system makes the H&E staining process more precise and
personal than ever before.
I need fast turnaround times and workflow efficiency.
• Free your technicians to focus on value-added work with
one-touch H&E slide processing that delivers true walkaway automation
• Optimise lean workflow opportunities with integration of
VENTANA VANTAGE workflow solution
I need to provide accurate results so physicians can make
accurate diagnoses.
• Count on consistent, reproducible results throughout the
day with fresh reagent application on every slide
• Improve diagnostic capability with excellent visualisation
of micro-anatomic detail
I need to reduce errors and mitigate risk.
• Ensure positive patient identification with barcoded
patient slides
• Protect against cross-contamination with individual slide
staining for every patient slide
• Reduce technician exposure to toxins with xylene-free
VENTANA SYMPHONY Clear
H&E | VENTANA SYMPHONY Fully Automated H&E System

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Specifications
General Characteristics
Capability
• Provides continuous loading of up to 500 slides at a time with throughput of approximately 160-200
slides per hour. Universal Slide tray holds up to 20 individual slides and can be stacked or “nested” for
pathologist review. “Slide Detect” ID—slide tracking supporting multiple barcode formats
• Simultaneous processing of multiple slide trays including drying, de-paraffinisation, staining
and coverslipping
Reagents
• All VENTANA SYMPHONY solutions are sealed, pre-packaged, ready-to-use and monitored with RFID
for inventory management. VENTANA SYMPHONY solutions can be changed “on the fly” without process
interruption
DI Water Supply
• NCCLS type II or type III DI water quality flow of greater than 650 ml/min (22 oz/min) while maintaining a
dynamic pressure of greater than 18.9 psi static pressure to 30 psi
Communication
• Standard category 5, RJ 45 network port, located within 426 cm (14’) of the left side of instrument
Network port with TCP/IP ports 80 and 443 open
Configuration
• Free standing
Certifications
• CSA (C and US indicators), EMC, CE and C-TICK compliant
Features
• CareGiver remote support—an automated remote monitoring and diagnostics solution that enables continuous monitoring and remote
service
• Fresh solutions are used for each slide, resulting in consistent and reproducible results
Environmental Requirements
Ventilation
• The facility ventilation duct should be 7.62 cm (3”) in diameter and be located between 31 cm to 457 cm
(1’ and 15’) of instrument. The ventilation flow specification must be a minimum of 35 cfm and a maximum
of 70 cfm
Liquid Waste Disposal
• Location of drain must be within 457 cm (15’) of right side of instrument and not exceed 91 cm (3’)
in height
Electrical Specifications
• Dedicated/isolated 20-amp circuit
• 230 ± 10% volts nominal AC, 50/60 Hz - NEMA 6-20R standard receptacle
Physical Characteristics
Space requirements
•
•
•
•
Recommended width is greater than 249 cm (98”). This will allow doors to open 180 degrees
Height must be greater than 219 cm (86”); (off casters, levelled and ventilated)
Depth must be greater than 131 cm (51.5”); this is spaced 13 cm (5") from wall
Instrument physical dimensions (H x W x D): 188 cm x 125 cm x 72 cm (74” x 49” x 28”)
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VENTANA SYMPHONY Fully Automated H&E System | H&E

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Product
Part Number Description
Quantity
VENTANA
SYMPHONY
Staining System
100799600
06114270001
Fully automated H&E staining platform individually processing patient samples
from drying through staining and glass coverslipping.
1
SYMPHONY B
900-204
05279496001
Intended to be used as a bluing reagent. When used in conjunction with
SYMPHONY N1 and N2+, SYMPHONY B will change the hue of nuclear
staining from purple to blue.
1L box
SYMPHONY C
900-202
05279470001
Intended for staining cytoplasmic and other non-nuclear components varying
shades of red to pink providing excellent differentiation between collagen,
smooth muscle and other eosinophilic structures.
1L box
SYMPHONY Clear
900-209
05279518001
Xylene-free solution used for the deparaffinisation and clearing of tissue
sections during the staining process.
2L bottle
SYMPHONY D
900-210
05279526001
Used for the differentiation of nuclear components stained with SYMPHONY
N2+ in regressive staining protocols.
1L box
SYMPHONY N1
900-201
05279461001
Intended for staining nuclei and other basophilic cell structures purple to dark
purple; used for progressive staining.
1L box
SYMPHONY N2+
900-213
05279542001
Intended for staining nuclei and other basophilic cell structures dark purple to
black; used for progressive and regressive staining.
1L box
SYMPHONY W
900-203
05279488001
Wash solution used in rinsing steps that occur between aqueous applications
during the staining process.
1L box
SYMPHONY
OPTISURE Glass
Coverslips
900-221
05448590001
Specialised glass coverslips applied following the staining and dehydration
steps of the VENTANA SYMPHONY staining process to provide enhanced
optical clarity and archivability to stained tissue sections. OPTISURE coverslips
have a dry mounting medium pre-applied and are packaged in cassettes that
are loaded onto the VENTANA SYMPHONY System.
1440
(12 cassettes of
120 coverslips)
SYMPHONY N2+
Cleaning Kit
900-217
05279593001
Used for cleaning SYMPHONY N2+ lines.
1 kit
SYMPHONY
Desiccant Filter
2094800
05258898001
Removes moisture from air, creating optimal humidity within the
coverslipping module.
1 filter
SYMPHONY Tray Kit
(25 mm)
900-301
05279631001
Replacement trays; each tray accomodates 20 slides.
5 trays/pack
SYMPHONY Tray Kit
(26 mm)
900-311
05279674001
Replacement trays; each tray accomodates 20 slides.
5 trays/pack
Better together |
Install the VENTANA SYMPHONY stainer with the VENTANA
VANTAGE workflow solution for complete chain of custody. Maximise
quality with ready-to-use, optimised reagents specially made for use on the
VENTANA SYMPHONY.
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Special Stains |
VENTANA NexES Special Stains System 19
Special Stains 22
Ancillary Reagents 33
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VENTANA NexES Special Stains System | SPECIAL STAINS
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VENTANA NexES Special Stains
System
The VENTANA NexES Special Stains system keeps staining processes
consistent and productive. The system offers proven reliability in the field,
and new features give you greater staining flexibility. This, in addition to a
broad menu of complex and routine special stains, gives you the power to
create greater efficiencies.
I need fast turnaround times and workflow efficiency.
• Walkaway automation ensures workflow efficiency
• Eliminate time wasted on manual reagent preparation
with ready-to-use reagent kits
• Improve workflow by freeing your most experienced
technicians for value-added tasks
• Count on pre-programmed protocol options to get
you up and running quickly and keep you operating
efficiently week after week
I need to provide consistent, high-quality stains so
physicians can make accurate diagnoses.
• Choose your optimal incubation time and counterstain
options for many stains
• Select alternate temperature batch mode operation, to
improve clarity and workflow for many stains
• Eliminate variability from technician-to-technician with
quality-controlled reagents
• Customise and save your best protocols from a menu
of options
I need to mitigate risk in my lab.
• Reduce technician exposure to harmful chemicals
• Ensure correct results with quality-controlled
reagent kits
SPECIAL STAINS | VENTANA NexES Special Stains System
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Specifications
Capability
• Special stains slide processing
Slide Carousel
• 1–20 slides
Reagent Carousel
• 25 reagent positions
Slides
• 25 x 75 mm, 1 x 3 inches or 26 x 76 mm Superfrost Plus
Bulk Solutions
• 2 bulk solutions
DI Water Supply
• NCCLS Type II water or equivalent (referred to as deionised water)
Temperature Control
• 37°C–70°C
Modularity
• 1–8 VENTANA NexES Special Stains systems may be controlled from one PC
Configuration
• Bench mounted with 20 liter waste bottle
Certifications
• CSA and CE compliant
Heat
• 1700 BTU
Room Temperature
• 15° C to 30° C (59° F to 86° F). Note: The instrument may be unable to maintain proper reaction
temperature if laboratory ambient temperature exceeds the specified maximum temperature.
Humidity
• 5% to 95%, non-condensing
Location
• Flat, level surface. Remove from sources of direct heat and moisture. Bulk fluid module can be placed
up to one metre above or below staining module.
Voltage
• 120/230 VAC ± 10%
Current
• 5/8 amps
Frequency
• 50/60 Hz
Staining Module (SM)
Bulk Fluid Module (BFM)
Size (W x D x H)
• 40.5 cm x 51 cm x 34 cm
(16” x 20” x 13.5”)
• 34 cm x 37 cm x 34 cm
(13.5” x 14.5” x 13.5”)
Weight
• 18 kg (40 lbs.)
• 13.6 kg (30 lbs.)
Clearances
• Top 38.1 cm (15”)
• Sides 10.2 cm (4”)
• Rear 15.2 cm (6”)
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VENTANA NexES Special Stains System | SPECIAL STAINS
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Product
Part Number
Description
VENTANA NexES Special
Stains
Full System 220V
F-SS-950100
05284791001
Fully automated special stains system applying pre-packaged, ready-to-use test kits. Full
system order volume includes a) NexES staining module; b) NexES computer system; c) flat
screen; d) E-Bar label printer and e) HP printer.
VENTANA NexES Special
Stains
Add-on Module(s)
F-SS-950101
05284805001
Fully automated special stains system applying pre-packaged, ready-to-use test kits. Add-on
module order volume includes one NexES staining module.
VENTANA NexES Special Stains Accessories
Product
Part Number
Description
Heavy Metal Extraction
System
860-104
05279453001
Heavy Metal Extraction System
Vapour Extraction Filter
860-101
05279445001
Vapour Extraction Filter
Waste Container with Sealed
Cap
1200800
05244820001
Waste Container with Sealed Cap
Reagent Tray
1826900
05253578001
Reagent Tray
E-Bar II Barcode Slide Label System and Accessories
Product
E-Bar II Barcode Slide
Label System
Part Number
1632800
05250862001
Description
E-Bar electronic barcode labelling and printing system - printer ~100-240 v, 50/60 Hz.
Cable, Parallel Interface
Power Cord (US/CAN)
Power Cord (INTL)
Printer Ribbon, E-Bar
Head Cleaning Pen
1347100
1490500
1490100
1632900
1490000
Insert Sheet, Quick Setup
Insert Sheet, Ordering Information
E-Bar Mylar Shields (5 shields/pkg)
Power Supply
E-Bar Label Kit
E-Bar Label Kit
1418702
05248850001
5 rolls of 500 labels and 5 mylar shields. Printer ribbon not included.
E-Bar Printer Ribbon
1632900
05250889001
Prints 8,100 labels.
Labels
1358501
05247829001
1 roll of 500 labels.
1490400
1490300
1829000
1490200
1418702
Better together |
Maximise your lab’s overall performance—integrate VENTANA NexES
Special Stains system easily with the VENTANA BenchMark series of
instruments, the VENTANA SYMPHONY individual slide stainer and the
VENTANA VANTAGE workflow solution.
SPECIAL STAINS | Special Stains
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Special Stains
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Stains for Mucins:
Alcian Blue, Mucicarmine, PAS, Alcian Blue for PAS, Diastase for PAS, Light Green for PAS
Congo Red Tsaining
Part Number
Control
Components/Storage
Quantity
860-002 05279186001
Colon
Alcian Blue Stain
NFR Counterstain
75 tests
15º-30º C
15º-30º C
Description
Alcian Blue Staining Kit demonstrates weakly acidic mucopolysaccharides. At pH 2.5, Alcian Blue stains sulphated mucins, usually
epithelial in origin (and usually PAS-negative), with some occurrences in connective tissue, e.g. in subcutaneous tissue in thyroid
deficiency, (myxedema) and in myxomas. It also stains carboxylated sialomucins found in the mucins of submaxillary glands, small intestine
and upper colon, and sulphated and carboxylated acid mucopolysaccharides. Some examples of components that stain positive with alcian
blue are goblet cells, hyaluronic acid (found in umbilical cord and connective tissue in dermis), salivary glands and gastric lining cells.
Alcian Blue (pH 2.5) may be helpful in detecting genetic disorders of acid mucosubstance metabolism and collagen diseases where various
acid mucosubstances are increased. The presence of acid mucosubstances tends to decline with advancing age.
Mucicarmine Staining Kit
Part Number
Control
Components/Storage
Quantity
860-011 05279275001
Bowel
Iron Hematoxylin A
Iron Hematoxylin B
Tartrazine Counterstain
Mucicarmine Stain
75 tests
2º-30º C
2º-30º C
2º-30º C
2º-8º C
Description
Mucicarmine Staining Kit is used to detect acid mucopolysaccharides (mucin). Mucin is a secretion of acid mucopolysaccharides
and other substances produced by a variety of epithelial and connective tissue cells. In certain inflammatory processes and certain
intestinal carcinomas, epithelial cells secrete excess mucin. Mucicarmine Staining Kit detects mucins of epithelial origin. Fibroblastic or
connective tissue mucins may stain poorly. The purpose of this stain is primarily to determine the site of a primary tumour or to distinguish
undifferentiated mucin-negative squamous cell lesions from mucin-positive adenocarcinomas. As is the case with current manual stains,
mucicarmine stain may not be appropriate for detection of metastatic cancers of the stomach. The stain may also be used as an aid in
the identification of Cryptococcus neoformans, a pathogenic fungus containing mucin in its capsule. Carmine and aluminium mordant
combine to stain the epithelial mucin a deep rose to red colour. Although the mechanism is not completely understood, it is believed that
the aluminium forms a chelate complex with carmine by dye‑like formation, to produce a net positive charge. The complex then attaches to
the acid groups of the mucin. Tartrazine Counterstain is applied to provide a contrasting yellow background. Mucin stains deep rose. Cell
nuclei stain black. Other tissue elements stain yellow by the counterstain. The capsule of Cryptococcus neoformans stains dark red.
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Special Stains | SPECIAL STAINS
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PAS Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
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860-014 05279291001
PAS with Hematoxylin
Liver
2º-30º C
Periodic Acid
2º-30º C
Neutralizer
2º-30º C
Hematoxylin Counterstain
2º-8º C
Schiff’s Reagent (3 vials)*
75 tests
*Note: Open only one Schiff’s Reagent vial at a time. Each vial is
stable for ONE month after opening.
Description
PAS Staining Kit is used to demonstrate the presence of glycogen in tissue. PAS-positive reticular fibres, basement membrane, fungus
and neutral mucopolysaccharides are also detected. PAS Staining Kit may also be used to aid in distinguishing a PAS-positive, secreting
adenocarcinoma from undifferentiated PAS-negative squamous cell carcinoma. PAS Staining Kit uses Periodic Acid to oxidise glycols to
aldehydes. Schiff’s Reagent forms a colourless dialdehyde compound that is transformed to the coloured final staining of glycol-containing
cellular components. PAS staining in tissue sections and digestion with diastase is useful as an aid in the diagnosis of glycogen storage
disease. The PAS Staining Kit stains glycogen bright magenta, with cell nuclei staining light purple.
Alcian Blue for PAS
Part Number
Type
Control
Components/Storage
Quantity
860-003 05279194001
PAS/Alcian Blue
Colon
Alcian Blue Stain
15º-30º C
75 tests
Note: This is not a stand-alone reagent; it must be used in
conjunction with PAS Staining Kit.
Description
Alcian Blue for PAS is an accessory reagent that is used with the PAS Staining Kit. In conjunction with the PAS Staining Kit, acid mucin
stains bright blue, while neutral polysaccharides stain bright magenta.
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Diastase Kit
Part Number
Type
Control
Components/Storage
Quantity
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860-004 05279208001
Diastase for PAS
Liver
Diastase
2º-8º C
75 tests
Description
Diastase is an enzyme which selectively digests (breaks down) glycogen. It is used to remove glycogen from tissue. This produces
“negative PAS” staining of the glycogen. Any remaining PAS staining after diastase digestion must be due to non-glycogen protein-bound
neutral polysaccharides, such as mucin or fungus. Diastase for PAS is an accessory reagent that is used with the PAS Staining Kit to
a) identify glycogen with certainty, by its removal and/or b) eliminate it from the tissue section when it is interfering with the identification
of other PAS-positive tissue structures. Because the glycogen is removed, the magenta staining is absent, but the nuclei still stain light
purple with the Hematoxylin Counterstain.
Light Green for PAS
Part Number
Type
Control
Components/Storage
Quantity
860-010 05279267001
PAS/Light Green
Liver
Light Green Counterstain
15º-30º C
75 tests
Description
Light Green for PAS is an accessory reagent that is used with the PAS Staining Kit. In conjunction with the PAS Staining Kit, fungal
elements stain magenta against a light green background.
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Stains for Connective Tissue:
Elastic, Jones, Jones Light Green , Reticulum II, Trichrome II Blue, Trichrome III Blue, Trichrome III Green
Elastic Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-005 05279216001
Elastic (Modified Hart’s)
Kidney, Lung, Skin
Elastic Clarifier
Decolorizer
Oxidizer
Elastic Tissue Stain
Van Gieson Solution
75 tests
15º-30º C
15º-30º C
15º-30º C
15º-30º C
15º-30º C
Description
Elastic Staining Kit demonstrates elastic fibres in tissue sections. Elastic Staining Kit is a modification of Hart’s method for elastic fibres.
A resorcin-fuchsin solution is used to stain elastic fibres dark blue or purple to black. Collagen appears pink to red. Van Gieson’s Solution
is applied to provide contrasting yellow background tissue. This stain is useful in demonstrating atrophy of elastic tissue in cases of
emphysema and the thinning and loss of elastic fibres in arteriosclerosis and other vascular diseases. In normal aorta, a majority of the
tissue is elastic and stains dark blue to purple. In kidney, the renal arteries will stain dark blue to purple, while the cortex will stain pink to
red. In lung, the pulmonary artery and bronchial walls contain elastin and will stain dark blue to purple. Under high power, the alveoli also
demonstrate an intricate meshwork of elastic fibres. The Elastic Staining Kit stains elastic fibres in tissue dark blue‑purple against a yellow
background. Collagen in the tissue appears pink to red.
Jones H&E Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-019 05279348001
Jones H&E (Hematoxylin and Eosin Counterstains)
Kidney
2º-30º C
Jones Toner
2º-30º C
Jones Fixer
2º-30º C
Jones Hematoxylin
2º-30º C
Jones Eosin
2º-8º C
Jones Periodic Acid
2º-8º C
Jones Silver A
2º-8º C
Jones Silver B
40 tests
Description
Jones Staining Kit demonstrates capillary basement membrane and is a modification of Jones Methanamine Silver procedure.
Methanamine in this stain is used to demonstrate the carbohydrate component of basement membrane. Periodic acid is used to oxidise
carbohydrates to aldehyde groups. Jones Silver A, a silver nitrate solution, provides the silver ions. Jones Silver B provides the alkaline
conditions that reduce the silver ions to metallic silver. Toner reagent contains gold chloride to form a more stable gold complex and to
remove the yellow tones from the tissue. Fixer, with thiosulfate, stops the reaction and removes any unreduced silver from the section.
The purpose of this stain is primarily to distinguish pathological abnormalities in kidney diseases. Hematoxylin and Eosin Counterstains
are applied to provide contrasting background. The Jones Staining Kit stains basement membrane black against a light pink background.
Basement membranes appear as black ink drawn lines and these fine lines should be seen in the glomeruli of the kidney.
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Jones Light Green Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-020 05279356001
Jones (Hematoxylin and Light Green Counterstains)
Kidney
2º-30º C
Jones Toner
2º-30º C
Jones Fixer
2º-30º C
Jones Hematoxylin
2º-30º C
Jones Light Green
2º-8º C
Jones Periodic Acid
2º-8º C
Jones Silver A
2º-8º C
Jones Silver B
40 tests
Description
Jones Light Green Kit demonstrates capillary basement membrane and is a modification of Jones Methanamine Silver procedure.
Methanamine in this stain is used to demonstrate the carbohydrate component of basement membrane. Periodic acid is used to oxidise
carbohydrates to aldehyde groups. Jones Silver A, a silver nitrate solution, provides the silver ions. Jones Silver B provides the alkaline
conditions that reduce the silver ions to metallic silver. Toner reagent contains gold chloride to form a more stable gold complex and to
remove the yellow tones from the tissue. Fixer, with thiosulfate, stops the reaction and removes any unreduced silver from the section. The
purpose of this stain is primarily to distinguish pathological abnormalities in kidney diseases. Hematoxylin and Light Green Counterstain
are applied to provide contrasting background. The Jones Light Green Kit stains basement membrane black against a light green
background. Basement membrane appear as black ink drawn lines and these fine lines should be seen in the glomeruli of the kidney.
Reticulum II Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-024 05279399001
Reticulum (Modified Gordon and Sweets)
Liver, Spleen
Oxidizer
Decolorizer
Sensitizer
Reticulum II Reducer
Toner
Fixer II
NFR Counterstain
Reticulum II Silver A
75 tests
2º-30º C
2º-30º C
2º-30º C
2º-30º C
2º-30º C
2º-30º C
2º-30º C
2º-8º C
Description
Reticulum II Staining Kit is used to demonstrate reticular fibres. The purpose of this stain is primarily to aid in the differential diagnosis of
certain tumour types. In certain tumours, reticulum is located in a characteristic position in relation to the actual tumour cells. Reticulum
stains can be used to show disease states in organs such as the liver, spleen and kidney by demonstrating reticular patterns not normal
to the organ. In normal liver, the fibres are well-defined strands, but in necrotic or cirrhotic liver, the fibres have discontinuous patterns.
Reticulum II Staining Kit is a modification of Gordon and Sweets Stain. Oxidizer, with potassium permanganate, oxidises the tissue
to enhance staining of reticular fibres. Decolorizer, with oxalic acid, removes excess potassium permanganate. Sensitizer, with ferric
ammonium sulphate, is added to form a metal-organic compound. The metal-organic compound is replaced by the silver in Reticulum II
Silver A. Reducer is applied to develop the deposited silver into visible silver. Toner reagent contains gold chloride for better contrast and
clarity. Fixer, with thiosulphate, stops the reaction and removes any unreacted silver from the section. Nuclear Fast Red counterstain is
applied to provide contrasting background. The Reticulum II Staining Kit stains reticular fibres black against a pink to red background.
26 NEXT>
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Trichrome II Blue Staining Kit
Part Number
Control
Components/Storage
Quantity
860-013 05279283001
Liver, Kidney, Skin, Colon
Bouins Solution
Hematoxylin A
Hematoxylin B
Trichrome Red
Trichrome Mordant
Trichrome Blue II
Trichrome Clarifier
75 tests
15º-30º C
15º-30º C
15º-30º C
15º-30º C
15º-30º C
15º-30º C
15º-30º C
Description
Trichrome II Staining Kit is used to study connective tissue, muscle and collagen fibres in tissue. Trichrome II Staining Kit is a modification
of Masson’s Trichrome Stain. Bouin’s Solution is applied to tissue sections to intensify the final colouration. Cytoplasm and muscle are
stained with Trichrome Red, containing Biebrich Scarlet and acid fuchsin. Nuclei are stained with iron hematoxylin. After application of
Trichrome Mordant, the collagen is stained with Trichrome Blue, which contains aniline blue. Trichrome Clarifier, an acetic acid solution, is
applied to create a more delicate and transparent shade of colour in the tissue section.
Trichrome III Blue Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-022 05279364001
Trichrome Blue (Modified Masson’s)
Bouin’s Solution
Bouin’s Solution 2
Trichrome III Hematoxylin A
Trichrome III Hematoxylin B
Trichrome III Enhancer
Trichrome III Red
Trichrome III Red 2
Trichrome III Mordant
Trichrome III Blue
Trichrome III Blue 2
75 tests
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
Description
Trichrome III Blue Staining Kit is used to study connective tissue, muscle and collagen fibres. Trichrome III Blue Staining Kit is a
modification of Masson’s Trichrome Stain. Bouin’s Solution is applied to tissue sections to intensify the final colouration. Cytoplasm and
muscle are stained with Trichrome Red, containing Biebrich scarlet and acid fuchsin. Nuclei are stained with iron hematoxylin. After
application of Trichrome III Mordant, the collagen is stained with Trichrome III Blue, which contains aniline blue. The Trichrome III Blue
Staining Kit stains collagen blue, muscle and erythrocytes red and cell nuclei blue‑black.
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Trichrome III Green Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-023 05279372001
Trichrome Green (Modified Masson’s)
Bouin’s Solution
Bouin’s Solution 2
Trichrome III Hematoxylin A
Trichrome III Hematoxylin B
Trichrome III Enhancer
Trichrome III Red
Trichrome III Red 2
Trichrome III Mordant
Trichrome III Green
Trichrome III Green 2
75 tests
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
2º-8º C
Description
Trichrome III Green Staining Kit is used to study connective tissue, muscle and collagen fibres. Trichrome III Green Staining Kit is a
modification of Masson’s Trichrome Stain. Bouin’s Solution is applied to tissue sections to intensify the final colouration. Cytoplasm and
muscle are stained with Trichrome Red, containing Biebrich scarlet and acid fuchsin. Nuclei are stained with iron hematoxylin. After
application of Trichrome III Mordant, the collagen is stained with Trichrome III Green, which contains fast green FCF. The Trichrome III
Green Staining Kit stains collagen green, muscle and erythrocytes red and cell nuclei blue to black.
28 NEXT>
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Stains for Micro-Organisms:
AFB III, Alcian Yellow, GMS II, Steiner
AFB III Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
860-027 05279437001
Acid-Fast Bacillus (Modified Ziehl-Neelson)
Tissue positive for acid-fast bacteria
15º-30º C
AFB Stain
15º-30º C
AFB Decolorizer II
15º-30º C
AFB III Blue
75 tests
Description
AFB III Staining Kit is used to selectively demonstrate mycobacterium and other acid fast organisms and components in tissue. AFB III
Staining Kit is a modification of the Ziehl-Neelsen stain. A carbol fuchsin solution is used to stain acid fast organisms and components red.
Aniline blue counterstain is applied to provide contrasting blue background.
Alcian Yellow Staining Kit
Part Number
Control
Components/Storage
Quantity
860-017 05279321001
Gastric tissue positive for H. pylori
2º-8º C
Alcian Yellow Toluidine Blue
2º-8º C
Alcian Yellow Sensitizer
2º-8º C
Alcian Yellow Oxidizer
2º-8º C
Alcian Yellow Stain
2º-8º C
Alcian Yellow Clarifier (3 vials*)
75 tests
*Note: Open only one Alcian Yellow Clarifier vial at a time. Each vial
is stable for ONE month after opening.
Description
Helicobacter pylori is a gram negative bacterium that has been shown to be the causative organism in some gastric and duodenal ulcers. It
is the most common cause of antral gastritis and has been linked to the development of the distal gastric carcinoma and low grade gastric
lymphoma. Helicobacter pylori has been shown to survive in the acid environment of the stomach and is able to destroy the neutral mucin
that the surface epithelial cells secrete. Identification in a gastric biopsy and prompt treatment with antibiotics can lead to a fast recovery
for the patient. Alcian Yellow Staining Kit stains the Helicobacter pylori blue, the background blue and the mucin yellow.
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GMS II Staining Kit
Part Number
Type
Control
Components/Storage
Quantity
<PREV
860-028 05412749001
Gomori’s Methanamine Silver (Modified)
Tissue positive for fungi or Pneumocystis carinii
2º-30º C
Oxidizer
2º-30º C
Neutralizer
2º-30º C
Toner
2º-30º C
Fixer
2º-30º C
Light Green Counterstain
2º-8º C
GMSII Silver A
2º-8º C
GMSII Silver B (2 vials)
75 tests
Description
GMS II Staining Kit selectively demonstrates polysaccharides in the cell walls of fungi and other opportunistic organisms, such as
Pneumocystis carinii. GMS Staining Kit is a modification of Gomori’s Methanamine Silver procedure. Tissue, fungal and microbial cell wall
polysaccharides are oxidised to aldehyde groups by GMS Oxidizer. Chromic acid suppresses weaker background staining of collagen fibres
and basement membranes. Neutralizer removes excess chromic acid. GMS Silver A, a silver nitrate reagent, provides the silver ions. GMS
Silver B provides the alkaline conditions that reduce the silver ions to metallic silver. Toner reagent contains gold chloride to form a more
stable gold complex and remove the yellow tones from the tissue. Fixer, with thiosulphate, stops the reaction and removes any unreduced
silver from the section. The purpose of this stain is primarily to distinguish pathogenic fungi, such as Aspergillus and Blastomyces as
well as other opportunistic organisms, such as Pneumocystis carinii in tissue sections. Light Green Counterstain is applied to provide
contrasting background. The GMS Staining Kit stains fungus black against a light green background. Aspergillus hyphae appear as black
filaments. Pneumocystis carinii organisms appear as clusters of helmet-shaped black spots.
Steiner Staining Kit
Part Number
Control
Components/Storage
Quantity
860-018 05279330001
Tissue positive for H. pylori, Cat Scratch, Spirochetes, Legionella,
2º-8º C
Steiner Diffuser
2º-8º C
Steiner Enhancer
2º-8º C
Steiner Reducer
2º-8º C
Steiner Silver
2º-8º C
Steiner Diluent
40 tests
Description
Steiner Staining Kit is used to aid in the identification of causative organisms of infection such as spirochetes, Legionella pneumophilia,
Helicobacter pylori and other bacteria. Organisms will stain dark brown to black. Background will stain golden yellow to amber. Other
tissue elements stain golden yellow to amber; nuclei will stain amber to dark brown.
Steiner Cleaning Kit
Part Number
Components/Storage
Quantity
860-025 05279402001
Steiner Clean
ambient
Probe Cleaning Solution
ambient
10 runs
Note: Steiner Cleaning Kit must be ordered with the Steiner Staining Kit. It is a critical component for proper
functioning of the VENTANA NexES Special Stains automated slide stainer
Description
Steiner Cleaning Kit is an accessory required for use with the Steiner Staining Kit. The Steiner Cleaning Kit is an alcohol solution that
is used to prevent and remove build-up of gum mastic residue from the VENTANA NexES Special Stains automated slide stainer that is
deposited during the Steiner assay.
30 NEXT>
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Other Special Stains:
Congo Red, Giemsa, Iron
Congo Red Staining Kit
Part Number
Control
Components/Storage
Quantity
860-026 05279429001
Tissue positive for Amyloid
Congo Red Stain
Glycine Buffer
Congo Red Hematoxylin
40 tests
2º-8º C
2º-8º C
2º-8º C
Description
Congo Red Staining Kit is used to selectively demonstrate amyloid in tissue sections. The staining reaction is based on the application
of congo red which stains the patterns of atypical proteins (amyloid). The b-pleated sheets of amyloid are suitable in size and shape to
accommodate the congo red molecules, which are held in the lattice work of the b-pleated sheets. Birefringence is an intrinsic property
of the amyloid fibril congo red complex. Congo red may also stain unexpected structures such as: keratin, elastic and dense collagen
fibres. Without both the red staining of congo red and the apple green birefrengence under polarisation a definite identification cannot
be made. The hematoxylin is applied as a contrasting blue nuclear stain. The Congo Red Staining Kit stains amyloid a bright pink to red
and nuclei blue. Amyloid may appear as circular or ribbon-like pink staining deposits throughout the tissue. It has a particular predilection
for deposition in blood vessel walls and basement membranes. Nuclear staining may vary from light to dark blue depending on the tissue
section and thickness (6-10 microns).
Giemsa Staining Kit
Part Number
Control
Components/Storage
Quantity
860-006 05279224001
Bone Marrow
Giemsa Stain
75 tests
15º-30º C
Description
Giemsa Staining Kit differentiates leucocytes in bone marrow and other hematopoietic tissue (lymph nodes). The stain can also be used
to demonstrate some micro-organisms, such as H. pylori. A buffered thiazine-eosinate solution is used to stain cells differentially with a
characteristic blue or pink colour. The Giemsa Staining Kit stains leucocytes purple, erythrocytes pink and mast cells and many other cell
types characteristically blue. Nuclei stain red to purple. The pathogenic gram-negative spiral bacteria, H. pylori, stains blue.
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Iron Staining Kit
Part Number
Control
Components/Storage
Quantity
860-009 05279259001
Liver
Iron Reagent A
Iron Reagent B
Nuclear Fast Red Counterstain
75 tests
<PREV
15º-30º C
15º-30º C
15º-30º C
Description
Iron Staining Kit detects the presence of ferric iron in tissue. The iron is separated from protein by hydrochloric acid. The free ferric
iron reacts with the potassium ferrocyanide to form an insoluble bright blue ferric ferrocyanide or Prussian blue. A Nuclear Fast Red
counterstain is applied to provide a pink to red contrasting background. Iron pigment stains bright blue. Heavy deposits of iron appear dark
blue. Cytoplasm stains pink and nuclei stain pink to red.
32 NEXT>
Ancillary Reagents | SPECIAL STAINS
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Ancillary Reagents
Product
Part Number
Description
Quantity
Special Stains Wash
Solution (10x)
860-015
05279305001
Special Stains Wash Solution is used to dilute the special stains reagents
that are dispensed onto the slide and to wash the slides in between reagent
applications on the VENTANA NexES Special Stains automated slide stainer.
It is used at a 1X concentration and therefore requires a 1:10 dilution prior
to use. The quality of the water used to prepare the Special Stains Wash
Solution is critical to obtaining proper staining results. De-ionised water is
recommended for its preparation.
2L bottle
(concentrate)
Liquid Coverslip
(Low Temperature)
250-009
05260981001
Liquid Coverslip is applied atop aqueous reagents by the VENTANA NexES
Special Stains automated slide stainer to prevent reagent evaporation and
ensure complete slide coverage.
2L bottle
(ready to use)
Special Stains
Cleaning Kit
860-016
05279313001
The Special Stains Cleaning Kit is used to neutralise and clean the reagent
aspiration and dispense mechanism of the VENTANA NexES Special Stains
system and prevent reagent carry-over. It is recommended to perform a
cleaning run at the end of each workday and after each Trichrome III Blue or
Trichrome III Green run.
Components: Cleaning Solution A, Cleaning Solution B, Cleaning Solution C
50 cleaning
cycles
Sections
AFB
Alcian Blue
Alcian Yellow
Congo Red
3-5µm
4µm
3-5µm
6-10µm
Elastic
Giemsa
GMS
Iron
4µm
4µm
4µm
4µm
Jones and Jones LG
Mucicarmine
PAS/Diastase
PAS/Alcian Blue
2-4µm
4µm
4µm
4µm
PAS
Reticulum
Steiner
Trichrome
4µm
2-5µm sections
< 4µm may cause light or
broken fibres
5µm for tissue sections
(< 5μm may cause light and pale staining)
2-3µm for biopsies (kidney/liver)
Water Quality
Type I
Type II
Type III
Conductivity at 25º C (microohms/cm)
0.1
1.0
10
Resistivity at 25º C (megaohms-cm)
10
1.0
0.1
Silicate (mg/L Si02)
0.05
0.1
1.0
pH
—
—
5.0-8.0
Bacteria Growth (cfu/mL)
10 (preferably bacteria free)
1000
n/a
Particulate Matter
0.22 mm filter
n/a
n/a
Organics
activated carbon
n/a
n/a
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IHC & ISH
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IHC & ISH |
VENTANA BenchMark ULTRA System 37
VENTANA BenchMark XT System 41
VENTANA BenchMark GX System 45
Detection Chemistries 49
Primary Antibodies by disease state 55
Primary Antibodies by alphabetical listing 68
Immunofluorescence Antibodies 179
Research Antibodies 183
Molecular Probes 185
Ancillary Reagents 193
Ventana part number in black, Roche item number in blue. The products that appear on this page are IVD (for in vitro diagnostic use).
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VENTANA BenchMark ULTRA | IHC & ISH
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VENTANA BenchMark ULTRA
Multi-Modal Slide-Staining System
The VENTANA BenchMark ULTRA system is the next generation in
IHC/ISH staining instrumentation. Using 30 individual slide processors,
its patient-centred workflow integrates multi-modal access for real-time
STAT processing. Turnaround times are significantly reduced resulting in
faster reporting of patient results, hence giving the next generation in lean
laboratory workflow.
The VENTANA BenchMark ULTRA is the first multi-modal
system to perform simultaneous IHC and ISH testing on
a single and random access platform. Multi-parameter
testing such as dual and triple stains may be performed
independently of other slides, allowing rapid results. The
VENTANA BenchMark ULTRA system is optimised for a full
range of ready-to-use immunohistochemical reagents, as
well as for a variety of in situ hybridisation tests, simplifying
complex molecular testing into a routine automated
procedure. The VENTANA BenchMark ULTRA system can
be linked to the existing Laboratory Information System
(LIS) to manage information from a single computer to
increase workflow efficiency and patient safety.
Proprietary flexible hardware and software
• 30 independently functioning slide staining chambers
preserve sample integrity and eliminate the need for
batch processing
• Add or remove individual slides at any time
• Access reagents at frequent intervals
• Run unexpected test requests as STAT without
interrupting cases already in process
• Count on visual and audio alerts to signal access to
slides and reagents
• Reagent Detect Sensor quickly and accurately reads
added reagent
Immediately process STAT and late-arriving samples
• Quickly respond to unexpected test requests without
impacting other cases in process
• Efficiently add reagents as needed to enable immediate
processing
• Ensure fast delivery of slides to pathologists visual
instrument interface
Provide consistent high-quality stains
• Optimise antibody and probe performance with
enhanced protocol flexibilities
• Efficiently process dual and triple IHC stains
• Deploy next-generation molecular tissue testing today
Eliminate bottlenecks
• Add samples throughout the day to eliminate the need
to batch slides
• Extend test order cut-off times, improving throughput
and productivity all day
• Free technicians’ time for value-added tasks using lean
workflow processes
Improve turnaround times and efficiency
• Deliver fast turnaround times with Lean Six Sigma
“single piece flow” processing
• Process all slides in each patient’s case together to
eliminate sorting
• See the exact status of patient samples with an intuitive
visual instrument interface
* 2009 Medical Design Excellence Award
IHC & ISH | VENTANA BenchMark ULTRA
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Specifications
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Fully Automated
• Baking, deparaffinisation, cell conditioning and staining, including IHC , ISH, SISH, Dual ISH,
immunofluorescence, multiparameter IHC staining (dual, triple), counterstain and titration
Slide Position
• 1–30 slides with independent processing/functionality and temperature control for each position
Reagent Carousel
Slides
Bulk Solutions
• Up to 7 different bulk solutions can be changed “on the fly” without process interruption
Waste
• Two – 20 litre carboys, can be changed “on the fly” without process interruption
DI Water Supply
• NCCLS Type II water or equivalent (deionised water)
Temperature Control
• Ambient to 100ºC
Modularity
• One to eight VENTANA BenchMark ULTRA (and/or VENTANA Discovery ULTRA) staining systems may
be controlled from one VENTANA BenchMark ULTRA computer system
Configuration
• Free standing
Certifications
• CSA
Features
• Automated IHC/ISH platform manages STAT requests with no impact to in-process slides
• Superior throughput – 90 slides per 8 hour shift
• Enhanced protocol flexibilities
Heat
• 400 BTU/Hr idle, 1000 BTU/Hr running
Room Temperature
• 20ºC to 32ºC (68ºF to 90ºF)
Humidity
Voltage
• ~ 230-240 / 100-120 V
Current
• 4/6 amps
Frequency
• 60/50 Hz
Size (W x D x H)
• 111.76 cm x 84.07 cm x 158.50 cm (44.00” x 33.10” x 62.40”)
Weight
• 294.8 kg (650 lbs.)
Clearances
• Top 38.1 cm (15”)
• Sides 10.2 cm (4”)
• Rear 15.2 cm (6”)
Better together |
Install the VENTANA BenchMark ULTRA stainer with the VENTANA
VANTAGE workflow system for complete specimen tracking. Maximise
stain quality with enhanced protocol flexibilities exclusive to the
VENTANA BenchMark ULTRA.
The products that appear on this page are IVD (for in vitro diagnostic use).
38 NEXT>
VENTANA BenchMark ULTRA | IHC & ISH
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Product
VENTANA
BenchMark
ULTRA Full
System
Part Number
Description
F-BKU-750 000 05465079001
750-602
F-ISH-200 001
F-IHC-250 005
750-600
1697400
2505700
322-001
05342724001
05280907001
05280893001
05342716001
05425485001
05424577001
05263212001
Part Number
Description
Full System, 220 V
Computer System
Flat Screen Monitor
Printer
Staining Module
Accessory Kit #1
Accessory Kit #2
Power Strip
1632800
1358501
1650800
1196700
06376525001
F-IHC-200 000
05250862001
05247829001
05250986001
05244676001
06376525001
05287693001
E-Bar II Barcode Slide Label System
Blank Flap-Label Roll
Waste Container and Cart (20L)
Graduated Carboy (20L)
Bottle Ship Kit
Multi-Plug 6 Positions Continental
1650800
1196700
06376525001
F-IHC-200 000
05250986001
05244676001
06376525001
05287693001
Bottle Ship Kit
VENTANA
BenchMark
ULTRA Add-on
Module
F-BKU-750 001 05465117001
750-600
1697400
2505700
322-001
05342716001
05425485001
05424577001
05263212001
Staining Module
Accessory Kit #1
Accessory Kit #2
Power Strip
VENTANA Lab
Manager, System
Disk
(For Central
Management
Capability)
2105200
05259002001
This networked software product provides single-point management of multiple advanced
staining systems. Customers with multiple hosts can share reagents and protocols. Singlehost customers can add a second host and start mulitiple runs at the same time.
VENTANA
Interface Point,
System Disk
(For VENTANA
LIS Connectivity
Capability)
1364000
05247861001
This networked product provides an interface between the VENTANA staining system and
the laboratory information system (LIS). The interface automates data entry at the stainer
and also provides for automatic status updates back to the LIS.
Add-on Module, 220 V
VENTANA BenchMark Series Accessories
Product
Part Number
Description
Dispenser Rack
321-044
06265553001
Reagent dispenser storage rack — grey
Graduated
Carboy
1196700
05244676001
20L receptacle for storage of diluted bulk solutions
Temperature Verifier 970-021
Slides (10)
05424615001
Used for independent testing of slide heater function; 10 slides
Waste Cart BMK
06141960001
Rolling cart used to hold waste container on VENTANA BenchMark series instruments
1488000
Product
Part Number
Description
E-Bar II Barcode
Slide Label
System
1632800
05250862001
E-Bar Label Kit
1418702
05248850001
E-Bar Printer
Ribbon
1632900
05250889001
Labels
1358501
05247829001
Power Cord (US/CAN)
Power Cord (INTL)
Head Cleaning Pen
1347100
1490500
1490100
1632900
1490000
Power Supply
E-Bar Label Kit
1490400
1490300
1829000
1490200
1418702
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40 Intelligent Systems. Intelligent Diagnostics. Intelligent Results.
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VENTANA BenchMark XT | IHC & ISH
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VENTANA BenchMark XT
Automated Slide Processing System
The VENTANA BenchMark XT system offers the flexibility needed to
expand your test menu, process more slides and improve your turnaround
times. Our fully automated random-access platform can simultaneously run
IHC and ISH tests, allowing you to process all patient cases in a single run.
I need faster turnaround times and greater efficiency.
• Increase your slide throughput by 25% and eliminate up
to 80% of manual tasks (compared to manual and semiautomated staining processes)
I need to provide consistent high-quality stains so
physicians can make accurate diagnoses.
• Improve consistency and reproducibility for IHC, ISH
and FITC applications on a fully automated platform with
precisely controlled, baking-through-staining technology
I need to free technicians’ time for value-added work.
• Eliminate manual steps associated with baking,
deparaffinisations and antigen retrieval with walkaway automation
• Simplify IHC and ISH processing through barcodedriven technology, coupled with pre-diluted antibodies
and probes
I need to mitigate risk and reduce errors in my lab.
• Eliminate the potential for errors with barcode-driven
technology that match protocols and reagents to
patient slides
I need greater flexibility.
• Process your IHC, ISH and FITC steps independently or
simultaneously
• Optimise your protocols with flexible options including
variable incubation times and temperatures
IHC & ISH | VENTANA BenchMark XT
Specifications
HOME
<PREV
Fully Automated
• Baking, deparaffinisation, cell conditioning and staining, including IHC, ISH, SISH, Dual ISH,
immunofluorescence, multiparameter IHC (dual, triple), counter stain and titration
Slide Tray BenchMark XT
• 1-30 slides with independent temperature control for each position
Reagent Carousel
Slides
Bulk Solutions
• Up to 7 different bulk solutions in 3 to 6-litre on-board containers
DI Water Supply
Temperature Control
• 37° C to 100° C ± 2.0° C
Modularity
• One to eight VENTANA BenchMark XT, VENTANA BenchMark GX, VENTANA NexES Special Stains or
VENTANA Discovery systems may be controlled from one PC (excluding VENTANA BenchMark ULTRA)
Configuration
• Free standing
Certifications
• CSA
Unique Features
• Fully automated, baking-through-staining technology
• 90 slides per 8–hr. shift
• Random-access platform – process ISH and IHC simultaneously
Heat
Room Temperature
Humidity
Voltage
• ~ 230-240 / 100-120 V
Current
• 4/6 amps
Frequency
• 60/50 Hz
Size (W x D x H)
• 88.9 cm x 66.0 cm x 153.0 cm (35” x 26” x 60.25”)
Weight
• 175 kg (385 lbs.)
Clearances
• Top 38.1 cm (15”)
• Sides 10.2 cm (4”)
• Rear 15.2 cm (6”)
Better together |
Install the VENTANA BenchMark XT platform with the VENTANA
VANTAGE workflow system for complete chain of custody. Maximise stain
quality with prediluted, optimised reagents specially made for use on the
VENTANA BenchMark XT.
42 NEXT>
VENTANA BenchMark XT | IHC & ISH
Product
Part Number
HOME
Description
Part Number
<PREV
NEXT>
Description
VENTANA
BenchMark XT
Full system
F-BXT-750 000
05281229001
Full System, 220 V
750-012
F-ISH-200 001
F-IHC-250 005
750-700
1963500
1981600
05264979001
05280907001
05280893001
05265231001
05256146001
05256577001
Computer System
Flat Screen Monitor
Printer
Staining Module
Accessory Kit #1
Accessory Kit #2
VENTANA
BenchMark XT
Add-on Module
F-BXT-750 001
05281237001
750-700
1963500
1981600
322-001
05265231001
05256146001
05256577001
05263212001
Staining Module
Accessory Kit #1
Accessory Kit #2
Power Strip
IHC VENTANA
BenchMark XT
Application
F-BXT-750 701
05281245001
Software
ISH VENTANA
BenchMark XT
Application
F-BXT-750 702
05281253001
Software
VENTANA Lab
Manager, System
Disk (For Central
Management
Capability)
2105200
05259002001
VENTANA
Interface Point,
System Disk
(For VENTANA
LIS Connectivity
Capability)
1364000
05247861001
322-001
1632800
1358501
1650800
1196700
F-IHC-200 000
06376517001
05263212001
05250862001
05247829001
05250986001
05244676001
05287693001
06376517001
Power Strip
E-Bar II Barcode Slide Label System
Blank Flap-Label Roll
Bottle Ship Kit, BenchMark XT/LT
F-IHC-200 000
06376517001
1650800
1196700
05287693001
06376517001
05250986001
05244676001
Bottle Ship Kit, BenchMark XT/LT
Product
Part Number
Description
Dispenser Rack
321-044
06265553001
Graduated
Carboy
1196700
05244676001
Slides (10)
05424615001
Waste Cart BMK
06141960001
1488000
Product
Part Number
Description
E-Bar II Barcode
Slide Label
System
1632800
05250862001
E-Bar Label Kit
1418702
05248850001
E-Bar Printer
Ribbon
1632900
05250889001
Labels
1358501
05247829001
Power Cord (US/CAN)
Power Cord (INTL)
Head Cleaning Pen
1347100
1490500
1490100
1632900
1490000
Power Supply
E-Bar Label Kit
1490400
1490300
1829000
1490200
1418702
HOME
<PREV
NEXT>
VENTANA BenchMark GX | IHC & ISH
HOME
<PREV
NEXT>
VENTANA BenchMark GX
Automated Slide Processing System
The VENTANA BenchMark GX automated slide preparation system offers
the flexibility you need to expand your test menu, process more slides and
improve your turnaround times. Best of all, this sophisticated and elegant
solution empowers you to manage multiple systems from a single computer.
Quality
Enhance staining quality, consistency and reproducibility in
a controlled, kinetically optimised reaction environment.
Productivity
Experience the benefits of walk away automation, increase
your slides-per-hour performance by up to 25% and
eliminate up to 80% of manual tasks.
Flexibility
Automate any or all slide preparation steps, from baking
through counterstaining. Process your SISH, IHC, ISH
or FITC slides and optimise your protocols with flexible
options.
set-up
set-up
baking through counterstaining
baking through counterstaining
VENTANA BenchMark GX
Slide Preparation System
run 1
0
1
2
Slide Stainer Only
run 2
3
4
5
6
7
8
8 hr. shift
run 1
set-up
cell conditioning (epitope retrevial)
dewax
baking
staining
counterstaining
IHC & ISH | VENTANA BenchMark GX
HOME
<PREV
Specifications
Fully Automated
• Baking, deparaffinisation, cell conditioning and staining, including IHC, ISH, SISH, Dual ISH,
immunofluorescence, multiparameter IHC (dual, triple), counter stain and titration
Slide Tray BenchMark GX
• 1-20 slides with independent temperature control for each position
Reagent Carousel
Slides
Bulk Solutions
• Up to 7 different bulk solutions in 3 to 6-litre on-board containers
DI Water Supply
Temperature Control
• 37° C to 95° C ± 2.0° C
Modularity
• One to eight VENTANA BenchMark GX, VENTANA BenchMark XT, VENTANA NexES Special Stains or
VENTANA Discovery systems may be controlled from one PC (excluding VENTANA BenchMark ULTRA)
Configuration
• Free standing
Certifications
• CSA
Unique Features
• Fully automated, baking-through-staining technology
• 60 slides per 8–hr. shift
Heat
• 1200 BTU/Hr running
Room Temperature
Humidity
Voltage
• ~ 200-240 / 100-120 V
Current
• 2/4 amps
Frequency
• 60/50 Hz
Size (W x D x H)
• Staining Module: 40.5 cm x 54.6 cm x 34.0 cm (16” x 21.5” x 13.5” )
• Automated Fluidics Module: 50.8 cm x 54.6 cm x 38.1 cm (20” x 21.5” x 15” )
• Base Cabinet Module: 50.8 cm x 54.6 cm x 52.1 cm (20” x 21.5” x 20.5” )
Weight
• Staining Module: 25 kg (55 lbs.)
• Automated Fluidics Module: 26.4 kg (58 lbs.)
• Base Cabinet Module: 13.6 kg (30 lbs.)
Clearances
• Top 38.1 cm (15”)
• Sides 10.2 cm (4”)
• Rear 15.2 cm (6”)
46 NEXT>
VENTANA BenchMark GX | IHC & ISH
HOME
<PREV
NEXT>
Product
VENTANA
BenchMark GX
Full System
VENTANA
BenchMark GX
Add-on Module
Part Number
Description
Part Number
Description
Full System, 220 V
750-012
F-ISH-200 001
F-IHC-250 005
750-850
750-861
750-872
100761200
0564979001
05280907001
05280893001
05894662001
05894638001
05894654001
05894689001
Computer System
Flat Screen Monitor
Printer
Staining Module
AFM
Waste Module
Drawer
322-001
1632800
1358501
1650800
F-IHC-200 000
750-ACC
05865379001
1196700
05263212001
05250862001
05247829001
05250986001
05287693001
05266017001
05865379001
05244676001
Power Strip
E-Bar II Slide Label System
Blank Flap- Label Roll
Accessory Kit
Bottle Ship Kit
1650800
F-IHC-200 000
750-ACC
05865379001
1196700
05250986001
05287693001
05266017001
05865379001
05244676001
Accessory Kit
Bottle Ship Kit
750-850
750-861
750-872
100761200
05894662001
05894638001
05894654001
05894689001
Staining Module
AFM
Waste Module
Drawer
VENTANA Lab
Manager, System
Disk
(For Central
Management
Capability)
2105200
05259002001
VENTANA
Interface Point,
System Disk
(For VENTANA
LIS Connectivity
Capability)
1364000
05247861001
Product
Part Number
Description
Dispenser Rack
321-044
06265553001
Graduated
Carboy
1196700
05244676001
Slides (10)
05424615001
Waste Cart BMK
06141960001
1488000
Product
Part Number
Description
E-Bar II Barcode
Slide Label
System
1632800
05250862001
E-Bar Label Kit
1418702
05248850001
E-Bar Printer
Ribbon
1632900
05250889001
Labels
1358501
05247829001
Power Cord (US/CAN)
Power Cord (INTL)
Head Cleaning Pen
1347100
1490500
1490100
1632900
1490000
Power Supply
E-Bar Label Kit
1490400
1490300
1829000
1490200
1418702
HOME
<PREV
Let CareGiver
safeguard your lab
Now available for VENTANA BenchMark ULTRA and
VENTANA SYMPHONY instruments, CareGiver remote
system performance monitoring protects your lab 24/7
with four fool-proof safeguards:
1. Reduce costly down time with remote troubleshooting, plus predictive intelligence that
identifies potential problems before they occur
2. Reduce cost of service with proactive preventive maintenance planning and ability to
proactively diagnose and solve existing and potential issues
3. Easily access industry experts with CareGiver using desktop sharing for application
support and user training
4. Receive timely and convenient software upgrades with convenient remote deployment
that minimises disruptions to your workflow
NEXT>
Detection Chemistries | IHC & ISH
HOME
<PREV
NEXT>
Detection Chemistries
AEC Detection Chemistry
AEC Detection Kit
Part Number
Quantity
Status
Components
760-020 05266041001
250 tests
IVD
Inhibitor
Biotinylated lg
Streptavidin HRP
AEC
AEC H202
IHC  ISH
Description
Basic AEC Detection Kit is an indirect biotin streptavidin system for detecting mouse and rabbit primary antibodies. The kit is intended for use
with formalin-fixed, paraffin-embedded tissue or frozen tissue. The AEC chromogen produces a dark red precipitate that is readily detected by
light microscopy. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated
slide stainers. Note: The AEC depositions are soluble in organic solvents. Aqueous mounting medium should be used.
IHC & ISH | Detection Chemistries
DAB Detection Chemistries
HOME
OptiView DAB IHC Detection Kit NEW
Part Number
Quantity
Status
Components
760-700 06396500001
250 tests
IVD
OptiView Peroxidase Inhibitor
OptiView HQ Universal Linker
OptiView HRP Multimer
OptiView DAB
OptiView H2O2
OptiView Copper
<PREV
IHC  ISH
Description
OptiView DAB IHC Detection Kit is an indirect, biotin-free system for detecting mouse IgG, mouse IgM and rabbit primary antibodies. The
OptiView detection system uses a proprietary, non-endogenous hapten system to provide enhanced sensitivity and specificity. OptiView’s exclusive
software gives users more control over their IHC protocol than ever before on the VENTANA BenchMark series automated slide stainers. This
control allows the OptiView detection system to be optimised for any need ranging from very low-expressing antigens to shorter turnaround times.
All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated slide stainers.
ultraView Universal DAB Detection Kit
Part Number
Quantity
Status
Components
760-500 05269806001
250 tests
IVD
ultraView Universal DAB Inhibitor
ultraView Universal HRP Multimer
ultraView Universal DAB Chromogen
ultraView Universal DAB H202
ultraView Universal DAB Copper
IHC  ISH
Description
ultraView Universal DAB is a multimer-technology based detection system intended for the specific and sensitive detection of mouse and
rabbit primary antibodies. The kit is biotin-free, thus it eliminates non-specific staining resulting from endogenous biotin. ultraView Universal
DAB consists of a robust chemistry that provides a clean background for the sensitive and specific visualisation of the targeted antigen and is
especially useful for the detection of antigens present in low concentrations. The primary antibody–HRP labelled antibody complex is visualised
using DAB resulting in a brown/black target signal. This detection system is optimised for use on the VENTANA BenchMark series automated
slide stainers. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated slide
stainers.
iVIEW DAB Detection Kit
Part Number
Quantity
Status
Components
760-091 05266157001
250 tests
IVD
iVIEW Inhibitor
iVIEW Biotinylated lg
iVIEW Streptavidin HRP
iVIEW DAB
iVIEW DAB H202
iVIEW Copper
IHC  ISH
Description
iVIEW DAB Detection Kit is an indirect biotin streptavidin system for detecting mouse and rabbit primary antibodies. The DAB chromogen
produces a dark brown precipitate that is readily visualised by light microscopy. All reagents are provided pre-diluted and ready-to-use for
convenient operation on VENTANA BenchMark series automated slide stainers.
50 NEXT>
Fast Red Detection Chemistries
ultraView Universal Alkaline Phosphatase Red Detection Kit
H O IHC
M E  ISH
<PREV
Part Number
Quantity
Status
Components
NEW
760-501 05269814001
250 tests
IVD
ultraView Red Alkaline Phosphatase
ultraView Red Enhancer
ultraView Red Naphthol
ultraView Red Fast Red A
ultraView Red Fast Red B
NEXT>
Description
ultraView Universal Red is a multimer-technology based detection system intended for the specific and sensitive detection of mouse and rabbit primary
antibodies. The kit is biotin-free, thus it eliminates non-specific staining resulting from endogenous biotin. ultraView Universal Red consists of a robust
chemistry that provides a clean background for the sensitive and specific visualisation of the targeted antigen and is especially useful for the detection
of antigens present in low concentrations. The primary antibody–alkaline phosphatase labelled antibody complex is visualised using Fast Red/Naphthol
resulting in a bright red target signal. This detection system is optimised for use on the VENTANA BenchMark series automated slide stainers. All
reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated slide stainers.
ultraView Red ISH DIG Detection Kit
Part Number
Quantity
Status
Components
IHC
800-505 05907128001
100 tests
IVD
ultraView Red ISH DIG Mouse anti-DIG Antibody
ultraView Red ISH DIG Multimer
ultraView Red ISH DIG pH Enhancer
ultraView Red ISH DIG Naphthol
ultraView Red ISH DIG Fast Red
ISH 
Description
ultraView Red ISH DIG Detection Kit is an indirect, biotin-free system for detecting DIG labelled probes. The kit is intended to identify
targets by chromogenic red in situ hybridisation (ISH) in sections of formalin-fixed, paraffin-embedded tissue that are stained on VENTANA
BenchMark series automated slide stainer. The clinical interpretation of any staining or the absence of staining, must be complemented by
histological examination and evaluation of proper controls. Evaluation must be made by a qualified reader within the context of the patient’s
clinical history and other diagnostic tests. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA
BenchMark series automated slide stainers.
ultraView Alkaline Phosphatase (AP) Red ISH Detection
Kit
Part Number
Quantity
Status
Components
800-504 05278929001
100 tests
IVD
ultraView AP Red ISH HybReady
ultraView AP Red ISH Multimer
ultraView AP Red ISH pH Solution
ultraView AP Red ISH Enhancer
ultraView AP Red ISH Fast Red
ultraView AP Red ISH Naphtol
IHC
ISH 
Description
The ultraView Alkaline Phosphatase (AP) Red ISH Detection kit is an indirect, biotin-free system for detecting rabbit anti-DNP antibodies. This kit,
in conjunction with a linking antibody, is used to identify DNP-labelled targets by in situ hybridisation (ISH) in sections of formalin-fixed, paraffinembedded tissue and yields a permanent red colour deposition at the site of probe-target interaction that is readily visualised by light microscopy.
The ultraView AP Red ISH Detection kit is based on multimer technology and is a complement to ultraView SISH Detection to enable routine dual ISH
staining. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated slide stainers.
IHC & ISH | Detection Chemistries
HOME
NBT-BCIP Detection Chemistries
ISH iVIEW Blue Detection Kit
Part Number
Quantity
Status
Components
800-092 05278511001
200 tests
IVD
iVIEW Blue Biotinylated Ig
ISH iVIEW Blue Anti-Fluorescein
iVIEW Blue Streptavidin Alkaline Phosphatase
iVIEW Blue Enhancer
iVIEW Blue NBT
iVIEW Blue BCIP
<PREV
IHC
ISH 
Description
ISH iView Blue Detection Kit is an indirect biotin streptavidin system for detecting fluorescein-labelled probes. The kit is intended for
use with formalin-fixed, paraffin-embedded tissue. ISH iView Blue Detection Kit utilises an alkaline phosphatase enzyme and NBT/BCIP
substrate chromogen reaction, which yields an intense blue permanent colour deposition at the site of probe-target interaction that is
readily visualised by light microscopy. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA
ISH iVIEW Blue Plus Detection Kit
Part Number
Quantity
Status
Components
IHC
760-097 05266181001
100 tests
IVD
ISH iVIEW Blue Plus HybReady
ISH iVIEW Blue Plus anti-DNP
ISH iVIEW Blue Plus Amp
iVIEW Blue Plus Biotinylated Ig
iVIEW Blue Plus Streptavidin Alkaline Phosphatase
iVIEW Blue Plus Enhancer
iVIEW Blue Plus NBT
iVIEW Blue Plus BCIP
ISH 
Description
ISH iVIEW Blue Plus Detection Kit is an indirect biotin streptavidin system for detecting DNP-labelled probes. The kit is intended for use with formalinfixed, paraffin-embedded tissue. ISH iVIEW Blue Plus Detection Kit utilises an alkaline phosphatase enzyme and NBT/BCIP substrate chromogen
reaction, which yields an intense blue permanent colour deposition at the site of probe-target interaction that is readily visualised by light microscopy.
All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated slide stainers.
52 NEXT>
HOME
Silver Detection Chemistries
ultraView SISH DNP Detection Kit
Part Number
Quantity
Status
Components
IHC
800-098 05907136001
100 tests
IVD
ultraView Silver ISH DNP Rabbit anti-DNP Antibody
ultraView Silver ISH DNP HRP
ultraView Silver ISH DNP Chromogen A
ultraView Silver ISH DNP Chromogen B
ultraView Silver ISH DNP Chromogen C
<PREV
NEXT>
ISH 
Description
ultraView SISH DNP Detection Kit is an indirect, biotin-free system for detecting DNP-labelled probes. The kit is intended to identify targets
by silver in situ hybridisation (SISH) in sections of formalin-fixed, paraffin-embedded tissue that are stained on VENTANA BenchMark
series automated slide stainer instruments. The clinical interpretation of any staining or the absence of staining, must be complemented by
histological examination and evaluation of proper controls. Evaluation must be made by a qualified reader within the context of the patient’s
clinical history and other diagnostic tests. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA
ultraView SISH Detection Kit
Part Number
Quantity
Status
Components
780-001 05271967001
100 tests
IVD
ultraView SISH HRP
ultraView SISH HybReady
ultraView Silver Chromogen A
ultraView Silver Chromogen B
ultraView Silver Chromogen C
IHC
ISH 
Description
ultraView SISH Detection Kit is an indirect, biotin-free system for detecting rabbit primary antibodies. The kit is intended to identify targets
by in situ hybridisation (ISH) in sections of formalin-fixed, paraffin-embedded tissue. This kit is to be used with INFORM HER2 DNA
probe, INFORM TOP2A DNA probe, INFORM EGFR DNA probe, INFORM MET DNA probe, INFORM Chromosome 7 probe and INFORM
Chromosome 17 probe. All reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series
automated slide stainers.
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Primary Antibodies by disease state | IHC & ISH
HOME
<PREV
NEXT>
Primary Antibodies
by disease state
Breast Diagnostics
Derm Diagnostics
Gastro-Intestinal and Colorectal Diagnostics
Lung Diagnostics
Lymphoma Diagnostics
Prostate Diagnostics
HOME
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<PREV
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Breast Diagnostics
Discover how our breast diagnostic solutions can change your
practice today.
Cornerstone breast markers
Emerging breast markers
•
•
•
•
•
•
•
•
•
•
• IGF-1R (G11)
• TAG-72 (B72.3)
• Topoisomerase IIa (JS5B4)
α-Smooth Muscle Actin (1A4)
Cytokeratin 5/6 (D5/16B4)
E-cadherin (36)
E-cadherin (EP700Y)
Estrogen Receptor (SP1)
GCDFP-15 (EP1582Y)
HER-2/neu (4B5)
Ki-67 (30-9)
p120 (98)
Progesterone Receptor (1E2)
Other important breast markers
•
•
•
•
•
•
Beta-catenin (14)
Calponin-1 (EP798Y)
MUC1 (H23)
p53 (BP-53-11)
p53 (DO-7)
p63 (4A4)
HOME
58
<PREV
NEXT>
HOME
<PREV
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Derm Diagnostics
We offer the most complete panel of derm tests on the market today, so you
can enable precise, personalised diagnoses.
Cornerstone derm markers
Emerging derm markers
•
•
•
•
•
•
•
•
•
•
•
• MITF (C5/D5)
• Melanoma Associated Antigen (KBA.62)
• Melanoma Associated Antigen (PLN2)
CD30 (Ber-H2)
CD34 (QBEnd/10)
CD45 (2B11&PD7/26)
CD45 (RP2/18)
Cytokeratin, Pan (AE1/AE3 & PCK26)
Factor XIIIa (AC-1A1)
Factor XIIIa (EP3372)
MART-1/melan A (A103)
Melanosome (HMB45)
S100 (4C4.9)
S100 (Polyclonal)
Other important derm markers
•
•
•
•
•
•
•
CD1a (EP3622)
CD4 (SP35)
CD7 (SP94)
CD8 (SP57)
CD68 (KP-1)
NGFR (MRQ-21)
Tyrosinase (T311)
IHC & ISH | Primary Antibodies by disease state
HOME
60 <PREV
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HOME
<PREV
NEXT>
Gastro-Intestinal and Colorectal
Diagnostics
Discover how our gastro-intestinal (GI) and colorectal cancer (CRC)
diagnostic solutions can change your practice today.
Cornerstone GI & CRC markers
Other important GI & CRC markers
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
Beta-catenin (14)
c-KIT (9.7)
CD2 (MRQ-11)
CDX-2 (EPR2764Y)
CEA (CEA31)
Chromogranin A (LK2H10)
COX-2 (SP21)
Cytokeratin 7 (SP52)
Epithelial Related Antigen (MOC-31)
Helicobacter pylori (SP48)
S100 (4C4.9)
S100 (polyclonal)
Synaptophysin (MRQ-40)
Villin (CWWB1)
CD45 (RP2/18)
Kappa (Polyclonal)
Lambda (Polyclonal)
p53 (DO7)
p63 (4A4)
Smooth Muscle Actin (1A4)
Emerging GI & CRC markers
• MUC2 (MRQ-18)
• MUC5AC (MRQ-19)
• MUC6 (MRQ-20)
Microsatellite instability markers
•
•
•
•
MLH1 (M1)
MSH2 (G219-1129)
MSH6 (44)
PMS2 (EPR3947)
HOME
62 <PREV
NEXT>
HOME
<PREV
NEXT>
Lung Diagnostics
Our cornerstone and emerging markers can help you to enable more
informed treatment decisions.
Cornerstone lung markers
Emerging lung markers
•
•
•
•
•
•
•
•
•
•
Chromogranin A (LK2H10)
Thyroid Transcription Factor-1 (8G7G3/1)
c-MET (SP44)
IGF-1R (G11)
MUC1 (H23)
Napsin A (polyclonal)
Other important lung markers
•
•
•
•
•
•
•
•
Calretinin (SP65)
CEA (CEA31)
CD99 (O13)
EGFR (3C6)
Ep-CAM (Ber-EP4)
Epithelial Related Antigen (MOC-31)
Ki-67 (30-9)
WT1 (6F-H2)
HOME
64
<PREV
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HOME
<PREV
NEXT>
Lymphoma Diagnostics
Our complete menu of cornerstone and novel tests help you enable
informed treatment decisions with stain sensitivity and consistency that
you—and patients—can count on.
Cornerstone lymphoma markers
Emerging lymphoma markers
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
•
bcl-2 (124)
CD3 (2GV6)
CD5 (SP19)
CD10 (SP67)
CD15 (MMA)
CD20 (L26)
CD23 (SP23)
CD30 (Ber-H2)
CD45 (2B11&PD7/26)
CD45 (RP2/18)
CD68 (KP-1)
CD138 (B-A38)
Cyclin D1 (SP4-R)
BOB.1 (SP92)
CD19 (SP119)
CD22 (SP104
CD71 (MRQ-48)
HGAL (MRQ-49)
LMO2 (1A9-1)
PD-1 (MRQ-22)
T-bet (MRQ-46)
Other important lymphoma markers
•
•
•
•
•
•
•
•
•
•
•
•
•
•
ALK1 (ALK01)
bcl-6 (GI191E/A8)
CD1a (EP3622)
CD4 (SP35)
CD7 (SP94)
CD8 (SP57)
CD56 (MRQ-42)
CD79a (SP18)
Kappa (polyclonal)
Lambda (polyclonal)
MUM1 (MRQ-43)
PAX5 (SP34)
TdT (polyclonal)
ZAP-70 (2F3.2)
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Prostate Diagnostics
In the PSA screening era, an estimated 50% of all prostate cancer is
over-treated. Our ERG IVD antibody provides additional information,
so you have confidence in your diagnosis.
Cornerstone prostate markers
Emerging prostate markers
•
•
•
•
•
•
• ERG (EPR3864)
Basal Cell Cocktail (34betaE12 + 4A4)
Cytokeratin (34betaE12)
p63 (4A4)
PSA (Polyclonal)
PSAP (PASE/4LJ)
IHC & ISH | Primary Antibodies by alphabetical listing
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Primary Antibodies
by alphabetical listing
a-1-Antichymotrypsin (ACT)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2604 05267196001
Rabbit Polyclonal
Histiocytic Tumours
Cytoplasmic
50 tests
Benign histiocytes
Description
a-1-Antichymotrypsin Rabbit Polyclonal Primary Antibody reacts with histiocytes and histiocytic neoplasms. Its major application is
defining the presence of a-1-Antichymotrypsin in histiocytes and tumours derived from them. In eosinophilic granuloma and malignant
histiocytosis, the reaction for this marker is heterogeneous in intensity and distribution. In fibrous histiocytomas, on the other hand, a
diffuse homogeneous reaction may be observed.
References
1. Isaacson P, et al., Lancet. 2:964-965, 1979.
2. Palmer PE, et al., Am J Clin Pathol. 62:350-354, 1974.
3. Palmer PE, et al., Cancer. 45:1424-1431, 1980.
4. Kindblom LG, et al., Hum Pathol. 13:834-840, 1982.
5. Raintoft I, et al., Hum Pathol. 10:419-424, 1979.
a-1-Antitrypsin (AAT)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2605 05267200001
Rabbit Polyclonal
Tonsil, Lymph Node, Appendix
Cytoplasmic
50 tests
Benign histiocytes
Description
a-1-Antitrypsin (AAT) Rabbit Polyclonal Primary Antibody is considered to be very useful in the study of inherited AAT deficiency, benign
and malignant hepatic tumours and yolk sac carcinomas. Positive staining for a-1-Antitrypsin may also be used in detection of benign and
malignant lesions of a histiocytic nature. Sensitivity and specificity of the results have made this antibody a useful tool in the screening of
patients with cryptogenic cirrhosis or other forms of liver disease with portal fibrosis of uncertain etiology.
References
1. Isaacson P, et al., Lancet. 2:964-965, 1979.
2. Palmer PE, et al., Am J Clin Pathol. 62:350-354, 1974.
3. Palmer PE, et al., Cancer. 45:1424-1431, 1980.
4. Kindblom LG, et al., Hum Pathol. 13:834-840, 1982.
5. Raintoft I, et al., Hum Pathol. 10:419-424, 1979.
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Primary Antibodies by alphabetical listing | IHC & ISH
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a-1-Fetoprotein (AFP)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2603 05267188001
Rabbit Polyclonal
Foetal Liver
Cytoplasmic
50 tests
Hepatocellular carcinoma
Description
Positive staining with AFP Rabbit Polyclonal Primary Antibody is seen in hepatocytes of foetal liver and hepatoma. Since only the traces of
AFP are found in adult serum, elevated levels suggest either a benign or malignant lesion of the liver, a yolk sac carcinoma or one of a few
other tumours. In conjunction with elevated serum levels, AFP has been immunohistochemically demonstrated in yolk sac carcinomas of
gonadal and extragonadal sites, in hepatic malignancies and a few other neoplasms. The antigen is not denatured by most fixatives.
References
1. Jacobsen GK, et al., Am J Surg Pathol. 5:257-66, 1981.
2. Peyrol S, et al., Digestion. 18:351-370, 1978.
3. Tsung SH, et al., Arch Pathol Lab Med. 101:572-574, 1977.
Actin, Muscle
(HUC1-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Goodman ZD, et al., Cancer. 55:124-135, 1985.
5. Roth LM, et al., Cancer. 37:812-820, 1976.
760-2502 05266882001
Mouse Monoclonal
Skeletal Muscle
Cytoplasmic
50 tests
IgG1
Large intestine and vessel smooth muscle
Description
Actin, Muscle (HUC 1-1) Mouse Monoclonal Primary Antibody may be used to aid in the identification of cells of normal and abnormal
myocytic lineage as an aid in diagnosis of anaplastic tumours. Actin, Muscle (HUC 1-1) antibody is directed against an epitope present on
muscle isoforms of actin, but not present on the cytoplasmic isoforms. Actin, at the ultrastructural level, consists of the diffusely distributed
cytoplasmic microfilaments that characterise muscle cells. Monoclonal antibodies specific to muscle actins have allowed accurate
localisation to cell types. The clone HUC 1-1 shows similar immunocytochemical and biochemical characteristics to those reported for
clone HHF35. In tissue sections, the HHF35 staining pattern and localisation (cardiac, skeletal as well as smooth muscle and myoepithelial
cells) roughly parallels desmin reactivity.
References
1. Sawtell NM, et al., Cell Motil Cytoskeleton. 11(4):318-25, 1988.
2. True LD, Atlas of Diag Immunohistopathology. 1990; JB Lippincott
Company Philadelphia.
3. Tsukada T, et al., Am J Pathol. 126(1):51-60, 1987.
4. Tsukada T, et al., Am J Pathol. 127(2):389-402, 1987.
5. Elias JM, Immunohistopathology. ASCP Press, Chicago, 1990.
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Actin, Muscle Specific
(HHF35)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2601 05267161001
Mouse Monoclonal
Skeletal Muscle
Cytoplasmic
50 tests
IgG1/K
Skeletal muscle
Description
Actin is a major component of the cytoskeleton. This antibody recognises actin of skeletal, cardiac and smooth muscle cells. It is not
reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three
distinctive components: alpha, beta and gamma in order of increasing isoelectric point. Muscle Specific Actin recognises alpha and
gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also
neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas and lymphomas are negative. This antibody is useful in the
identification of rhabdoid cellular elements.
References
1. Gown AM, et al., Am J Pathol. 125:191, 1986.
2. Schmidt R, et al., Am J Pathol. 131:199, 1988.
3. Azumi N, et al., Modern Pathology. 1:469-474, 1988.
Actin, Smooth Muscle
(1A4)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2833 05268303001
Mouse Monoclonal
Appendix, Uterus
Cytoplasmic
50 tests
IgG
Colonic and blood
Description
Actin is a major component of the cytoskeleton and is present in every cell type. Actin can be resolved on the basis of its isoelectric points
into three distinctive components: alpha, beta and gamma in order of increasing isoelectric point. Smooth Muscle Actin antibody does not
stain cardiac or skeletal muscle, but, it will stain myofibroblasts and myoepithelial cells. This antibody could be used together with Muscle
Specific Actin to distinguish leiomyosarcoma from rhabdomyosarcoma. In most cases of rhabdomyosarcoma, this antibody gives negative
results, whereas Muscle Specific Actin is positive in the rhabdomyoblasts. Leiomyosarcomas are positive with both Muscle Specific Actin
and Smooth Muscle Actin antibodies.
References
1. Cooke PH, J Cell Biol. 68:539-556, 1976.
2. Skalli O, et al., J Cell Biol. 103:2787-2796, 1986.
3. Gown AM, et al., J Cell Biol. 100:807-813, 1985.
4. Kuroda M, Biochem Biophys Acta. 843:20-213, 1985.
5. Lazarides E, J Histochem Cytochem. 223:507-528, 1975.
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Adrenocorticotropin (ACTH)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2708 05268176001
Rabbit Polyclonal
Normal Pituitary
Cytoplasmic
50 tests
Pituitary
Description
ACTH is a useful marker in classification of pituitary tumours. It reacts with ACTH-producing cells (corticotrophs). It also may react with
other tumours (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
References
1. Pizarro CB, et al., Braz J Med Biol Res. 37(2):235-43, 2004
2. Viacava P, et al., J Endocrinol Invest. 26(1):23-8, 2003.
3. Kageyama K, et al., Am J Med Sci. 324(6):326-30, 2002.
ALK1, CONFIRM
(ALK01)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Fan X, et al., J Histochem Cytochem. 50(11):1509-16, 2002
5. Japon MA, et al., J Clin Endocrinol Metab. 87(4):1879-84, 2002.
800-2918 05278783001
Mouse Monoclonal
Anaplastic Large Cell Lymphoma
Cytoplasmic, Nuclear
50 tests
IgG3/K
Anaplastic large cell lymphoma
Description
CONFIRM ALK1 (ALK01) Mouse Monoclonal Primary Antibody recognises a formalin resistant epitope in both the p80 protein, identified
as a hybrid of the anaplastic lymphoma kinase (ALK) protein, and the nucleophosmin (NPM) and the 200 kD normal ALK proteins. ALK1
(ALK01) antibody may also recognise any ALK protein chimeric produced as a result of variant translocations involving the ALK gene on
chromosome 2. ALK1 (ALK01) antibody recognises varying degrees of ALK expression in both the cytoplasm and nucleus of anaplastic
lymphoma cells. ALK protein expression is substantially specific for anaplastic lymphomas, except for weak expression in some normal
cells in the central nervous system including neurons, glial cells and endothelial cells. ALK expression may also be seen in inflammatory
myofibroblastic tumours and rare rhabdomyosarcomas.
References
1. Pulford K, et al., Blood. 89(4):1394-404, 1997.
2. Griffin CA, et al., Cancer Res. 59(12):2776-80, 1999.
3. Falini B, et al., Am J Pathol. 153(3):875-86, 1998.
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Annexin A1
(MRQ-3)
Part Number
Type
Control
Localisation
Quantity
Isotype
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760-4435 05973945001
Mouse Monoclonal
Hairy Cell Leukaemia
Cytoplasmic, Membranous
50 tests
IgG1
Spleen, hairy cell leukaemia
Description
Annexin (ANXA1) is strongly expressed on the cell membrane and occasionally in the cytoplasm of tumour cells in 97% of samples from
patients with hairy cell leukaemia. By contrast, B-cell lymphomas other than hairy cell leukaemia, including typical splenic lymphoma
with villous lymphocytes and patients with variant hairy cell leukaemia—as defined by current morphologic, phenotypic and clinical
criteria—are ANXA1-negative. In a study by Falini, et al. ANXA1 immunodetection was 100% sensitive and specific for hairy cell leukaemia.
Normal B cells from different lympho-hemopoietic tissues were ANXA1-negative. In this study the expression of ANXA1 in myeloid
cells, macrophages or T-cell subset served as positive control. These findings validated the results of gene expression profiling in hairy
cell leukaemia at the protein level by showing that ANXA1 is consistently expressed in this type of leukaemic disease, but not in other
B-cell lymphomas. Negativity for ANXA1 was also present in patients with splenic lymphoma with villous lymphocytes, variant hairy cell
leukaemia, prolymphocytic leukaemia, marginal zone and lymphoplasmacytoid lymphomas. Thus, ANXA1 is a molecule specific to hairy
cell leukaemia that can be used to differentiate this disease from other B-cell lymphomas. Wang, et al. showed that high ANXA1 expression
is frequent in oesophageal and oesophagogastric junction adenocarcinomas, is associated with more advanced pathologic T stage and the
presence of distant metastasis and is an independent prognostic factor for patient survival.
References
1. Falini B, et al., Lancet. 363(9424):1869-70, 2004. Erratum in: Lancet.
363(9427):2194, 2004.
2. Wang KL, et al., Clin Cancer Res. 12(15):4598-604, 2006.
3. Xia SH, et al., Oncogene. 21(43):6641-8, 2002.
4. Dreier R, et al., Histochem Cell Biol. 110(2):137-48, 1998.
Basal Cell Cocktail NEW
(34βE12+4A4)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4536 06364497001 (50 tests);
790-1010 06419445001 (250 tests)
Mouse Monoclonal
Normal Basal Cells in Prostate Tissue
4A4 = Nucleus, 34βe12 = Cytoplasm
50 Tests (790-4536), 250 Tests (790-1010)
IgG2a/K
Prostate carcinoma
Description
VENTANA Basal Cell Cocktail (34βE12+p63) Mouse Monoclonal Primary Antibody is an antibody cocktail of p63 (4A4) and keratin
(34βE12). p63 (4A4) antibody reacts with the p63 molecule in the nuclei of human prostatic basal cells and urothelial tissues. Keratin
(34βE12) antibody reacts with cytokeratins 1, 5, 10 and 14 and stains the cytoplasm of human prostatic basal cells. This antibody cocktail
may be used to aid in the differentiation of benign and malignant prostatic lesions.
References
1. Shah RB, et al., Am J Clin Pathol. 122:517-523, 2004.
2. Zhou M, et al., Am J Surg Pathol. 27:365–371, 2003.
3. Shah RB, et al., Am J Surg Pathol. 26:1161–1168, 2002
4. Yang A, et al., Mol Cell. 2:305-316, 1998.
5. Dairkee SH, et al., J Natl Cancer Inst. 80:691-695, 1988.
6. Gown AM, et al., Amer J Clin Pathol. 84:413-424, 1985.
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BCA-225
(Cu-18)
Part Number
Type
Control
Localisation
Quantity
Isotype
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760-2607 05267226001
Mouse Monoclonal
Breast Carcinoma
Cytoplasmic
50 tests
IgG1
in situ and invasive breast carcinoma
Description
BCA-225 (Cu-18) Mouse Monoclonal Primary Antibody recognises a human breast carcinoma associated glycoprotein BCA-225 (220-225
kD). This protein differs in size and distribution from other breast carcinoma antigens. Unlike other antibodies against breast carcinoma
antigens, this antibody does not react with benign or malignant colonic, stomach, prostate, liver, pancreas, thyroid or parotid tissues.
Adenocarcinomas of the lung, ovary and endometrium also stain with this antibody.
References
1. Ceriani RL, et al., Monoclonal Ab’s and Breast Cancer Boston,
Martinus, Nijhoff, 1985.
2. Mesa-Tejada R, et al., Am J Pathol. 130:305-314, 1988.
bcl-2, CONFIRM
(124)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Loy TS, et al., Am J Clin Pathol. 96(3):326-9, 1991.
4. Ma CK, et al., Am J Clin Pathol. 99(5):551-7, 1993.
790-4464 05986826001
Mouse Monoclonal
B Cells of the Mantle Zone, Interfollicular T Cells in Tonsil.
Cytoplasmic
50 tests
IgG1
Lymphoma
Description
CONFIRM bcl-2 (124) Mouse Monoclonal Primary Antibody is directed against human bcl-2 expressed by B cells of the mantle zone
and interfollicular T cells. This antibody exhibits a cytoplasmic staining pattern and may be used to aid in the identification of follicular
lymphomas and diffuse large cell lymphomas, and to differentiate follicular lymphomas from reactive lymph nodes.
References
1. Chao DT, et al., Annu Rev Immunol. 16:395-419, 1998.
2. Pezzella F, et al., N Engl J Med. 329:690-4, 1993.
3. Campos L, et al., Blood. 81:3091-6, 1993.
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bcl-6
(GI191E/A8)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4241 05269008001
Mouse Monoclonal
Tonsil, Lymph Node
Nuclear
50 tests
IgG1
Tonsil
Description
bcl-6 is a transcriptional regulator gene which codes for a 706-amino acid nuclear zinc finger protein. Antibodies to this protein stain the
germinal centre cells in lymphoid follicles, the follicular and interfollicular cells in follicular lymphoma, diffuse large B-cell lymphomas
and Burkitt’s lymphoma, as well as the majority of the Reed-Sternberg cells in nodular lymphocyte predominant in Hodgkin’s disease.
In contrast, bcl-6 (GI191E/A8) Mouse Monoclonal Primary Antibody rarely stains mantle cell lymphoma and MALT lymphoma. bcl-6
expression is seen in approximately 45% of CD30+ anaplastic large cell lymphomas but is consistently absent in other peripheral T-cell
lymphomas.
References
1. Dogan A, et al., Am J Surg Pathol. 24(6):846-852, 2000.
2. Shaffer AL, et al., Immunity. 13:199-212, 2000.
Beta-Catenin
(14)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Kraus Md, et al., Am J Surg Pathol. 24(8):1068-78, 2000.
4. Carbone A, et al., Blood. 90(6):2445-2450, 1997.
760-4242 05269016001
Mouse Monoclonal
Fibromatosis of Breast or Abdomen
Nuclear
50 tests
IgG1
Breast carcinoma
Description
Beta-catenin is a 92 kD protein normally found in the cytoplasm of the cell in the submembranous location. This protein is associated with
E-Cadherin and may be essential for the function of E-Cadherin. Mutations in the Beta-catenin gene result in nuclear accumulation of this
protein. Nuclear accumulation of this protein has been demonstrated in fibromatosis lesions of the breast and abdomen and therefore is
useful in differentiating this lesion from other spindle cell lesions that may occur in these locations. Nuclear accumulation of Beta-catenin
has also been demonstrated in colorectal carcinoma.
References
1. Alman BA, et al., Am J Pathol. 151(2):329-34, 1997.
2. Li C, et al., Am J Pathol. 153(3):709-14, 1998.
3. Kuhnen C, et al., Pathol Rex Pract. 196(5):299-304, 2000.
4. Abraham SC, et al., Hum Pathol. 33(1):39-46, 2002.
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BG8, Lewisy
(F3)
Part Number
Type
Control
Localisation
Quantity
Isotype
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760-4450 05973767001
Mouse Monoclonal
Lung Adenocarcinoma
Cytoplasmic
50 tests
IgG1
Adenocarcinoma of colon
Description
Blood group antigens have been examined as potential discriminators between pulmonary adenocarcinoma (PACA) and epithelioid
mesotheloma (EM). Lewisy is the only one of these that appears to have some merit. BG8 is raised from the SK-LU-3 lung cancer line and
its ability to distinguish between PACA and EM was first reported by Jordon and colleagues in 1989. Three groups have since reported
their results. These studies included 231 cases of PACA and 197 cases of EM. Sensitivity and specificity for PACA were both 93%. Yaziji H,
et al. reported a sensitivity of non-mesothelial antigens for adenocarcinoma as organ dependent, with BG8 performing at 98% in the breast
cancer group and 100% in the lung cancer group. The specificity of the non-mesothelial (non-EM) antigens for adenocarcinoma was 98%
for BG8. They concluded using logical regression analysis that a three-antibody immunohistochemical panel including calretinin, BG8 and
MOC-31 would provide 96% sensitivity and specificity for distinguishing EM from adenocarcinoma from a variety of sources (lung, ovary,
breast, stomach).
References
1. Davidson B, et al., Virchows Arch. 435(1):43-9, 1999.
2. Jordan D, et al., Am J Pathol. 135(5):931-7, 1989.
3. King JE, et al., Histopathology. 48(3):223-32, 2006. Review.
4. Marchevsky AM, et al., Appl Immunohistochem Mol Morphol.
15(2):140-4, 2007.
5. Ordonez NG, et al., Am J Surg Pathol. 27(8):1031-51, 2003.
BOB.1 NEW
(SP92)
Part Number
Type
Control
Localisation
Quantity
6. Ordonez NG, et al., Am J Surg Pathol. 24(4): 598-606, 2000.
7. Pan CC, et al., Appl Immunohistochem Mol Morphol. 13(4):34752, 2005.
8. Yaziji H, et al., MMod Pathol. 19(4):514-23, 2006.
760-4593 06433308001
Rabbit Monoclonal
Tonsil
Nuclear; Cytoplasmic
50 tests
Follicular lymphoma
Description
BOB.1 (SP92) is used as an aid in the identification of B-cells in germinal centres and mantle cells and differentiation of lymphomas within
the context of an antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
References
1. Steimle-Grauer SA, et al. Virchows Arch. 442:284-293, 2003.
2. Valsami S, Pappa V, et al. Haematologica. 92(10):1343-50, 2007.
3. Stein H, et al. Blood. 97:496-501, 2001.
4. Hertel CB, et al. Oncogene. 21:4908-4920, 2002.
5. Pileri SA, et al. Am J Pathology. 162:243- 253, 2003.
6. Hoefnagel JJ, et al. Moder Pathology. 19 :1270-1276, 2006.
7. Greiner A, et al. Am J Pathol. 156:501-507, 2000.
8. Kuroda H, et al. Breast Cancer. 14(3):317-22, 2007.
9. Saez A-I, et al. Mod Pathol. 15(3):21-220, 2002.
10. Loddenkemper C, et al. J Pathol. 202:60-69, 2004.
11. Hoeller S, et al. Histopathology. 56, 217- 228, 2010.
12. Browne P, et al. Am J Clin Pathol 120:767-777, 2003.
13. Gibson SE, et al. Am J Clin Pathol. 126:916-924, 2006.
c-KIT (CD117), PATHWAY FDA approved
(9.7)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-2951 05278317001
Rabbit Monoclonal
GIST, Skin, Breast
50 tests
IgG
Membranous — GIST
Description:
PATHWAY c-KIT (9.7) Rabbit Monoclonal Primary Antibody is intended for the qualitative detection of KIT protein in gastro-intestinal
stromal tumours (GISTs). It is indicated as an aid in the selection of GIST within the context of the patient’s clinical history, tumour
morphology and other diagnostic tests evaluated by a qualified pathologist. It may be used after the diagnosis of GIST as an aid in the
selection of GIST patients who may qualify for imatinib mesylate (Gleevec/Glivec) therapy. PATHWAY c-KIT (9.7) antibody is optimised for
use on the VENTANA BenchMark series automated slide stainers and for manual application in combination with iVIEW DAB Detection
Kit and accessories. The clinical interpretation of any staining or the absence of staining, must be complemented by morphological studies
and evaluation of proper controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and
other diagnostic tests.
The Role of KIT Gene Mutations in the Development of GISTs:
In their landmark 1998 publication, Dr. Hirota and his colleagues established not only that GISTs express KIT, but that KIT gene mutations are
present in these tumours. Furthermore, they showed that the resulting mutant KIT isoforms demonstrate kinase activity in the absence of stem
cell factor, the natural ligand for KIT. These observations have been confirmed by a growing number of groups and it is now established that
KIT mutations are present in >85% of GISTs. The majority of mutations occurs in exon 11 (65-70% of GISTs) and includes a wide range of
deletions, insertions, point mutations or combinations thereof. An insertion/reduplication of six base pairs in exon 9 is found in ~15% of GISTs,
almost exclusively in those arising in the small intestine. Mutations also occur in exons 13 and 17, but are much rarer. Regardless of the exon
involved, KIT gene mutations in GISTs are invariably in-frame and, when cloned and expressed in vitro, have constitutive kinase activation.
Moreover, phosphorylation of KIT is consistently detectable in GIST tumour extracts, supporting a direct role for KIT in intracellular signaling.
Clinical Significance:
GISTs arise predominantly in the stomach (60%) and small intestine (25%), but also occur in the rectum (5%), oesophagus (5%) and a
variety of other locations (5%), including appendix, gall bladder, mesentery and omentum. Affected patients range in age from the teens
to the 90’s, but the majority are older and the peak is around age 60. A slight male predilection has been observed in most studies. There
are no solid figures on the true incidence of GISTs, but it is estimated that ~4,500 new cases are diagnosed each year in the United States.
In 1998, Drs. Seichi Hirota (Osaka University) and Lars-Gunnar Kindblom (University of Gothenburg) each independently observed that
GISTs express the receptor tyrosine kinase KIT (CD117). Their observations provided a clue to the possible cell of origin for GISTs, namely
the interstitial cells of Cajal (ICC). These inconspicuous, dendritic-like cells are widely distributed throughout the muscularis propria of the
oesophagus, stomach, small and large bowel. They play an important role in gut motility by regulating slow-wave contractions. Like GISTs,
ICC express KIT and the majority are also positive for CD34. The hypothesis that GISTs are pathogenetically related to ICC in the gut wall,
as proposed by both Dr. Hirota and Dr. Kindblom, is now widely accepted. Subsequent studies from a large number of different laboratories
have confirmed that KIT is the single most specific marker of GISTs. Immunodetectable KIT is present on the cell surface and/or cytoplasm
of GIST tumour cells in approximately 90% of cases.
Highly Sensitive and Specific:
The scientifically advanced rabbit monoclonal antibody technology of PATHWAY c-KIT clone 9.7 is the source of optimised assay sensitivity
and specificity. Directed against the c-terminal domain of the c-KIT protein, PATHWAY c-KIT recognises both wild-type and mutant forms
of the c-KIT protein. PATHWAY c-KIT (9.7) specifically binds to antigen located in the cell membrane and cytoplasmic regions of mast
cells, interstitial cells of Cajal (ICC) and GIST cells. The advanced performance of the PATHWAY c-KIT rabbit monoclonal antibody and
standardised slide preparation assist the pathologist with the clear differentiation of positives and negatives in this qualitative assay. The
following are examples of staining for c-KIT in GIST and physiologic tissues (intestine) using PATHWAY c-KIT.
References
1. Blechman J, et al., J. Biol. Chem. 228:(6)4399-4406, 1993
2. Corless C, et al., Am. J. of Pathol. 160:1567-1572, 2002.
3. Fletcher C, et al., Human Pathol. 33:459-465, 2002.
4. Heinrich MC, et al., J. Clin. Oncol. 20:1692-703, 2002.
5. Heinrich M, et al., Proceedings of ASCO. 21:2a(abstract #6), 2002.
6. Hirota S, et al., Int. J .Clin. Oncol. 6:1-5, 2001.
7. Hirota S, et al., Science. 279:577-80, 1998.
8. Hirota S, et al., Am. J. Surg. Pathol. 24:326-7, 2000.
9. Isozaki K, et al., Am. J. of Pathol. 157:1581-5, 2000.
10. Kindblom LG, et al., Am. J. Pathol. 152:1259-69, 1998.
11. Maeyama H, et al., Gastroenterology. 120:210-5, 2001.
12. Miettinen M, et al., Virchows Arch. 438:1-12, 2001.
13. Nishida T, et al., Nat. Genet. 19:323-4, 1998.
14. Rubin BP, et al., Int. J. Surg. Pathol. 8:5-10, 2000.
15. Rubin BP, et al., Cancer Res. 61:8118-8121, 2001.
16. Taniguchi M, et al., Cancer Res. 59:4297-300, 1999.
17. Yarden Y, et al., EMBO Journal. 6:3341-3351, 1987.
76 Primary Antibodies by alphabetical listing | IHC & ISH
c-MET (Total), CONFIRM
(SP44)
Part Number
Type
Control
Localisation
Quantity
790-4430 05571219001
Rabbit Monoclonal
Kidney, Gastric Carcinoma, Non-Small Cell Lung Carcinoma
50 tests
Colon carcinoma
Description
CONFIRM Total c-MET (SP44) Rabbit Monoclonal Primary Antibody is directed against a membranous and/or cytoplasmic epitope
present in human normal epithelial or tumour cells. This antibody may be used to aid in the identification of normal and neoplastic c-MET
expressing cells.
References
1. Prat M, et al., Int J Cancer. 49(3):323-328, 1991.
2. Comoglio PM, et al., Nature Reviews Drug Disc. 7(6):504-516, 2008.
C3d NEW
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Lutterbach B, et al., Cancer Research. 67(5):2081-2088, 2007.
4. Engelman JA, et al., Science. 316(5827):1039-1043, 2007.
760-4522 06419143001
Rabbit Polyclonal
Acute Rejected Kidney Transplant
Membranous, Cytoplasmic
50 tests
IgG
Description
Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and
alternative complement activation pathways. C3d deposition in the renal transplant PTCs (peritubular capillaries) is indicative of AR (acute
rejection) with subsequent high probability of graft loss. C3d antibody, combined with C4d antibody, can be utilised as a tool for diagnosis
of AR and warrant prompt and aggressive anti-rejection treatment.
References
1. Bickerstaff A, et. al., Am J Pathol, 173:347-357, 2008.
2. Kuypers DR, et. al., Transplantation, 76:102-108, 2003.
3. Eggertsen G, et. al, APMIS, 109:825-834, 2001.
4. Pfaltz K, et. al., J Cutan Pathol. 37:654-658, 2010.
HOME
C4d
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4436 05973937001
Rabbit Polyclonal
Lymph Node, Tonsil, Kidney (Reject Transplant)
50 tests
IgG
Kidney, acute rejection
Description
C4d is a stable split product remnant of classical complement activation which becomes covalently bound to endothelium and basement
membrane after induction of the classical antibody-induced pathway. As an established marker of antibody-mediated acute renal allograft
rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in both chronic renal allograft
rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection and borderline rejection. It has been shown to be
a significant predictor of transplant kidney graft survival and is therefore an aid in treating acute rejection.
References
1. Jianghua C, et al., Clin Transplant. 19(6):785-91, 2005.
2. Kayler LK, et al., Transplantation. 85(6):813-20, 2008.
3. Ranjan P, et al., Nephrol Dial Transplant. 23(5):1735-41, 2008.
4. Nadasdy GM, et al., Hum Pathol. 36(11):1178-85, 2005.
CA 19-9
(121SLE)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Seemayer CA, et al., Nephrol Dial Transplant. 22(2):568-76, 2007.
6. Bouron-Dal Soglio D, et al., Hum Pathol. 39(7):1103-10, 2008.
760-2609 05267242001
Mouse Monoclonal
Colon, Salivary Gland
Cytoplasmic
50 tests
IgM
Malignant gastro-intestinal carcinoma
Description
CA 19-9 antigen is highly expressed in gastro-intestinal (gastric, pancreatic and colonic) adenocarcinomas and salivary gland
mucoepidermoid carcinomas. CA 19-9 (121SLE) Mouse Monoclonal Primary Antibody is usually not reactive with breast, kidney and
prostate carcinomas.
References
1. Gatalica Z, et al., Applied IHC. 2(3):205-211, 1994.
2. Encabo G, et al., Bulletin Cancer (Paris). 73:256-9, 1986.
3. Basso D, et al., Med Sci Res. 17:13-4, 1989.
4. Tabuchi Y, et al., Cancer. 66:1529-33, 1990.
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CA 125
(OC125)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-2610 05267269001
Mouse Monoclonal
Ovarian Carcinoma
50 tests
IgG1/K
Serous adenocarcinoma of ovary
Description
CA-125 reacts with malignant ovarian epithelial cells. The antigen is formalin resistant permitting the detection of ovarian cancer by
immunohistochemistry, although serum assays for this protein are widely used to monitor ovarian cancer. CA-125 also reacts with antigens
in seminal vesicle carcinoma and anaplastic lymphoma.
References
1. Dabawat S, et al., Int J Gyn Path. 2:275-285, 1983.
2. Davis H, et al., Cancer Res. 46:6143-6148, 1986.
3. Nouwen E, et al., Cancer Res. 46:866-876, 1986.
Calcitonin
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
4. Quirk J, et al., J Obst Gyn. 159:644-649, 1988.
5. Fukazawa I, et al., Gynecol Obstet. 243:41-50, 1988.
760-2611 05267277001
Rabbit Polyclonal
Medullary Carcinoma of Thyroid
Cytoplasmic
50 tests
Medullary carcinoma of thyroid
Description
Immunohistochemical staining with Calcitonin Rabbit Polyclonal Primary Antibody has proven to be an effective way of demonstrating
calcitonin-producing cells in the thyroid. C-cell hyperplasia and medullary thyroid carcinomas stain positive for calcitonin. Studies of
calcitonin have resulted in the identification of a wide spectrum of C-cell proliferative abnormalities.
References
1. Copp DH, et al., Endocrinology. 70:638-649, 1962.
2. Kameda Y, et al., Cell Tissue Res. 206:403-415, 1980.
3. Coombes RC, et al., Lancet. 1:1080-1083, 1974.
4. Dayal Y, et al., Cancer. 43:1331-1338, 1979.
5. DeLellis RA, et al., Am J Clin Pathol. 7(4):587-294, 1978.
HOME
Caldesmon
(E89)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4375 05463459001
Rabbit Monoclonal
Smooth Muscle from Bowel, Oesophagus, Uterus
Cytoplasmic
50 tests
IgG
Breast, sclerosing adenosis
Description
Caldesmon is a regulatory protein found in smooth muscle and other tissues which interacts with actin, myosin, tropomyosin and
calmodulin. Caldesmon (E89) Rabbit Monoclonal Primary Antibody labels smooth muscle and tumours of smooth muscle, myofibroblastic
and myoepithelial differentiation. Caldesmon antibody has also been used to differentiate epithelioid mesothelioma from serous papillary
carcinoma of the ovary.
References
1. Comin CE, et al., Am J Surg Pathol. 31(8): 1139-48, 2007.
2. Watanabe K, et al., Hum Pathol. 30(4): 392-6, 1999.
3. McCluggage WC, Adv Anat Pathol. 11(3): 162-71, 2004.
Calponin-1
(EP798Y)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Miettinen M, et al., Arch Pathol Lab Med. 130(10): 1466-78, 2006.
5. Comin CE, et al., Am J Surg Pathol. 30(4): 463-9, 2006.
760-4376 05435684001
Rabbit Monoclonal
Smooth Muscle from Bowel, Oesophagus, Uterus
Cytoplasmic
50 tests
IgG
Description
Calponin is a 34 kD polypeptide that interacts with actin, tropomyosin and calmodulin. It is involved in smooth muscle contraction
mechanism and is restricted exclusively to smooth muscle tissue. Calponin-1 (EP798Y) Rabbit Monoclonal Primary Antibody has been
found to be useful in differentiating benign sclerosing lesions of the breast from carcinoma. Calponin positivity has also been noted in
malignant myoepithelioma and pleomorphic adenoma of salivary gland origin, as well as angiomatoid malignant fibrous histiocytoma.
References
1. Wang NP, et al., Appl Immunohistochem. 5(3):141-151, 1997.
2. Nagao T, et al., Cancer. 83(7):1292-9m, 1998.
3. Savara AT, et al., Mod Pathol. 10(11):1093-1100, 1997.
4. Fanburg-Smith JC, et al., Hum Pathol. 30(11):1336-43, 1999.
5. Hornick JL, et al., Am J Surg Pathol. 27(9): 1183-96, 2003.
6. Werling RW, et al., Am J Surg Pathol. 27(1): 82-90, 2003.
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Calretinin, CONFIRM
(SP65)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
790-4467 05992184001
Rabbit Monoclonal
Mesothelioma, Normal Appendix
50 tests
Pleura, malignant mesotheliona
Description
CONFIRM Calretinin (SP65) Rabbit Monoclonal Primary Antibody is directed against the calcium binding protein calretinin, that is
expressed by normal and reactive mesothelium, eccrine glands of skin, Sertoli cells of the testis, ovarian stromal cells and adrenal cortical
cells. This antibody exhibits a nuclear and cytoplasmic staining pattern and may be used to aid in the identification of mesothelioma and in
distinguishing mesothelioma from adenocarcinoma.
References
1. Kuznicki J, et al., Biochem J. 308(Pt 2):607–612, 1995.
2. Andressen C, et al., Cell Tissue Res. 271:181–208, 1993.
3. Rogers JH, J Cell Biol. 105(3):1343-53, 1987.
4. Lugli A, et al., Hum Pathol. 34(10):994-1000, 2003.
5. Cao QJ, et al., Int J Gynecol Pathol. 20:346-52, 2001.
6. Oates J, et al., Histopathology. 36(4):341-7, 2000.
7. Doglioni C, et al., Am J Surg Pathol. 20:1037-46, 1996.
8. Nagel H, et al., Pathol Res Pract. 194:759-64, 1998.
9. Ordonez NG, Mod Pathol. 11:929-33, 1998.
10. Leers MP, et al., Histopathology. 32:209-16, 1998.
11. McCluggage WG, et al., Histopathology. 38(5):403-8,2001.
12. Nogales FF, et al., Int J Gynecol Pathol. 21(1):34-40, 2002.
13. Cappello F, et al., Pathology. 33(2):142-8, 2001.
14. Attanoos RL, et al., Histopathology. 37(3):224-31, 2000.
15. Gotzos V, et al., Am J Surg Pathol. 23:701-11, 1999.
Carcinoembryonic Antigen (CEA) NEW
(CEA31)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4594 06433316001
Mouse Monoclonal
Adenocarcinoma of Colon, Colon Mucosa
Cytoplasmic
50 tests
IgG1
Colorectal carcinoma
Description
CEA (CEA31) Mouse Monoclonal Primary Antibody used as an aid in the identification and diagnosis of adenocarcinomas within the
context of an antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
References
1. Go, VLW et al. Cancer. 37:562-566, 1976.
2. Delellis, RA et al. Am J Clin Pathol 50:587-594, 1978.
3. Kamino, H et al. Cancer. 61:1142-1148, 1988.
4. Tron, V et al. Arch Pathol Lab Med. 111:291-293, 1987.
5. Abutaily, AS et al. J Clin Pathol. 55(9):662-8, 2002.
6. Bhatnagar, J et al. Anticancer Res. 22(3):1849-57, 2002.
7. Carella, R et al. Am J Surg Pathol. 25(1):43-50, 2001.
8. Lagandijk, JH et al. J Clin Pathol. 52(4):283-90, 1999.
HOME
CD1a NEW
(EP3622)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4525 06419160001
Rabbit Monoclonal
Thymus
Membranous
50 tests
IgG1
Description
CD1a, a protein of 43 to 49 kD, has been shown to be expressed on dendritic cells and cortical thymocytes. Langerhans cells in the skin
and some epithelia also express this protein. This antigen is expressed on cells comprising Langerhans cell histiocytosis and Langerhans
cell sarcoma. CD1a (EP3622) Rabbit Monoclonal Primary Antibody has been used to differentiate various cutaneous lymphomas (T-cell)
from B-cell lymphomas and pseudolymphomas.
References
1. Angel CE, et al., Blood. 113:1257-67, 2009.
2. Emile JF, et. al., Am J Surg Pathol. 19:636-641, 1995.
3. Stefano AP, et. al., Br J Haematol. 105:394-41, 1999.
4. Han X, et. al., Am J Clin Pathol, 127:578-544, 2007.
CD2
(MRQ-11)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Pomplun S, et. al., Histopathol, 40:152-158, 2002.
6. Alexiev BA, et. al., Diagnostic Pathology, 2:13, 2007.
7. Adachi Y, et. al., Pathol Intl., 58 :169-173, 2008.
760-4377 05463467001
Mouse Monoclonal
Tonsil, Lymph Node
50 tests
IgG1/K
Tonsil
Description
CD2 is one of the earliest T cell lineage restricted antigens to appear during T cell differentiation and only a rare CD2+ cell can be found
in the bone marrow. It is found in all T-lymphocytes and natural killer cells but not in B cells or any other cell population. CD2 can be
considered a pan-T cell antigen and is therefore useful for the identification of virtually all normal T-lymphocytes. It is also very useful in the
assessment of lymphoid malignancies as it is expressed in the majority of precursor and post-thymic lymphomas and leukaemias, and is
not expressed by B neoplasms (Foon & Todd, 1986). As with other pan-T cell antigens, CD2 may be aberrantly deleted in some neoplastic T
cell population, especially peripheral T cell lymphomas. Rarely slg+ B cell neoplasms have been described to form spontaneous E-rosettes
but these reactions are not mediated via the CD2 receptor (Knowles, 1989).
References
1. Aguilera NS, et al., Arch Pathol Lab Med. 130(12):1772-9, 2006.
2. Barrionuevo C, et al., Appl Immunohistochem Mol Morphol.
15(1):38-44, 2007.
3. Bovenschen HJ, et al., Br J Dermatol. 153(1):72-8, 2005.
4. Foon KA, et al., Blood. 68:1-31, 1986.
5. Gonzalez L, et al., Journal of Comparative Pathology. 125:41-7,
2001.
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CD3, CONFIRM
(2GV6)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
790-4341 05278422001
Rabbit Monoclonal
Tonsil, Spleen
50 tests
Tonsil
Description
CONFIRM CD3 (2GV6) Rabbit Monoclonal Primary Antibody (IgG) is directed against the non-glycosylated epsilon chain of the human CD3
molecule. CD3 is expressed in the membrane and cytoplasm of normal and neoplastic T cells. The CD3 antigen is first detectable in early
thymocytes and its appearance probably represents one of the earliest signs of commitment to the T cell lineage. This antibody detects
both normal and neoplastic T cells.
References
1. Chetty R, et al., J Pathol. 173(4): 303-307, 1994.
2. Clark EA, et al., Immunology Today. 10(7): 225-28, 1989.
3. Clevers H, et al., Eur J Immunol. 18(5): 705-710, 1988.
4. Clevers H, et al., Ann Rev of Immunology. 6: 629-662, 1988.
5. Campana D, et al., J Immunol. 138(2): 648-655, 1987.
CD4, CONFIRM
(SP35)
Part Number
Type
Control
Localisation
Quantity
6. Dennings SM, et al., Oxford University Press, Oxford-New YorkTokyo, 1987: 144-147.
7. Beverley, PC, et al., Eur J Immunol. 11(4): 329-334, 1981.
8. Meuer SC, et al., Nature. 303(5920): 808-810, 1983.
9. Mason DY, et al., J Clin Pathol. 42:1194-1200, 1989.
790-4423 05552737001
Rabbit Monoclonal
Tonsil, Liver
Membranous
50 tests
Lymphoma
Description
CONFIRM CD4 (SP35) Rabbit Monoclonal Primary Antibody is intended for the qualitative detection of CD4 in sections of normal
and neoplastic human tissues. CD4 is present on helper-inducer T-lymphocytes that recognise antigen in the context of MHC class 2
molecules. CD4 positive staining results may aid in identifying T-cell lymphomas and in identifying the T-helper-inducer cell subset of
T-lymphocytes in normal tissues.
References
1. Reinherz EL, et al. Proc. Natl. Acad. Sci. USA. 76(8):4061-5, 1979.
2. Brady RL, et al. Curr. Topics Microbiol. Immunol. 205:1-18, 1996.
3. Doyle C, et al. Nature. 330(6145):256-9, 1987.
4. Dalgleish AG, et al., Nature. 312(5996):763-7, 1984.
5. Garcia-Herrera A, et al., J Clin Oncol. 26(20):3364-71, 2008.
6. Maddon PJ, et al., Cell. 42(1):93-104, 1985.
HOME
CD5, CONFIRM
(SP19)
Part Number
Type
Control
Localisation
Quantity
<PREV
790-4451 05929903001
Rabbit Monoclonal
Tonsil, Lymphoma
Cytoplasmic, Cell Membrane
50 tests
Lymphoma
Description
CONFIRM CD5 (SP19) Rabbit Monoclonal Primary Antibody is directed against human CD5 expressed on the plasma membrane of virtually
all human T cells and the B1a subset of human B cells found in the follicular mantle zones, bone marrow and peripheral blood. Staining CD5
is commonly used as part of several IHC panels to determine T cell and B cell sub-classifications. CD5 antibody may be used to aid in the
identification of T cell lymphomas and certain B cell lymphomas, including mantle cell lymphoma.
References
1. Dalloul A, Autoimmun. 8:349-353, 2009.
2. Dono M, et al., Int J Biochem Cell Biol. 36:2105-2111, 2004.
3. De Leon ED, et al., Mod Pathol. 11:1046-1051, 1998.
CD7 NEW
(SP94)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Cabezudo E, et al., Hematologica. 84:413-418, 1999.
5. Matolcsy A, et al., Am J Pathol. 147:207-216, 1995.
6. Suguro M, et al., Cancer Sci. 97:868-874, 2006.
790-4558 06537847001
Rabbit Monoclonal
Tonsil, Thymus
Membranous
50 tests
Rabbit Ig
T-cell lymphoma
Description
CD7 (SP94) Rabbit Monoclonal Primary Antibody is directed against the 40kD transmembrane glycoprotein CD7, which is present in
thymocytes and mature T cells. CD7 (SP94) antibody may be used to aid in the identification of T cell lymphomas.
References
1. Sempowski GD, et al. Crit Rev Immunol. 19:331-348, 1999.
2. Cotta AC, et al. Appl Immunohistochem Molec Morphol. 14:
291-295, 2006.
3. Al Saati T, et al. Appl Immunohistochem Molec Morphol. 9:
289-296, 2001.
4. Ormsby A, et al. J Am Acad Dermatol. 45:405–413, 2001.
5. Inaba T, et al. Leuk Lymphoma. 42:1161-1171, 2001.
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CD8, CONFIRM
(SP57)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
790-4460 05937248001
Rabbit Monoclonal
Tonsil, T cell lymphoma
Cell Membrane
50 tests
T-cell lymphoma
Description
CONFIRM CD8 (SP57) Rabbit Monoclonal Primary Antibody is intended for the qualitative detection of CD8 in sections of normal and
neoplastic human tissues. The CD8 glycoprotein is present on cytotoxic/suppressor T-lymphocytes that recognise antigen in the context of
MHC class 1 molecules. CD8 positive staining results may aid in identifying T cell lymphomas and in identifying the T cytotoxic/suppressor
cell subset of T-lymphocytes in normal tissues.
References
1. Reinherz EL, et al., Immunol Today. 4:5-8, 1983.
2. Reinherz EL, et al., Nature. 294:168-170, 1981.
CD10 NEW
(SP67)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Perussia B, et al., J Immunol. 131:223-231, 1983.
4. Bierer BE, et al., Ann Rev Immunol. 7:579-599, 1989.
790-4506 05857856001
Rabbit Monoclonal
Tonsil
50 tests
Rabbit Ig
Lymphoma
Description
CD10 (SP67) Rabbit Monoclonal Primary Antibody is directed against the CD10 molecule or common acute lymphoblastic leukaemia
antigen (CALLA), expressed on the surface of early lymphoid cells and on various non-lymphoid tissues including breast myoepithelial
cells, bile canaliculi, fibroblasts, kidney tubular brush borders and small intestine epithelium. This antibody exhibits a cell membranous
and/or cytoplasmic staining pattern and may be used to aid in the identification of Burkitt’s lymphoma and follicular germ cell lymphoma,
and in the classification of some carcinomas such as renal cell carcinoma.
References
1. Gregory CD, et al. Apoptosis. 4:11-20, 1999.
2. Shipp MA, et al. Proc Natl Acad Sci USA. 85:4819-4823, 1988.
3. Kaufmann O, et al. Am J Clin Pathol. 111:117-122, 1999.
4. Shipp MA, et al. Proc Natl Acad Sci USA. 86:297-301, 1989.
5. Arber DA, et al. Appl Immunohistochem. 5:125-140, 1997.
6. Chu P, et al. Appl Immunohistochem & Molec Morphol. 8:257262, 2000.
7. Avery A, et al. Am J Surg Pathol. 24:203-210, 2000.
HOME
CD14 NEW
(EPR3653)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4523 06419151001
Rabbit Monoclonal
Tonsil
50 tests
IgG
Description
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently
has been shown to bind apoptotic cells. CD14 antibody labels Kupffer cells in liver sinusoids. In lymphoid tissues, dendritic cells are
distinctly stained. Most other normal tissues are negative. This antibody labels monocyte-macrophages and Langerhans cells in
Langerhans cell histiocytosis. Tumour cells are positive for CD14 in monocytic leukaemia and true histiocytic lymphomas. Sinusoidal
histiocytes express CD14 and CD169, whereas most of the other monocyte-derived cells in reactive lymph node lack these markers. CD14
antibody labels numerous diffuse large B-cell lymphomas and splenic marginal zone lymphoma, but not other B-cell lymphomas.
References
1. Gregory CD, et. al., Apoptosis. 4:11-20, 1999.
2. Larregina AT, et. al., Nature Immunol. 2:1151-1158, 2001.
3. Ziegler-Heitbrock HW, et. al., Immunol Today. 14:121-125, 1993.
4. Steiniger B, et. al., Immunology. 92:307-316, 1997.
5. Buckley PJ, et. al., Am J Pathol 128:505-520, 1987.
CD15, CONFIRM
(MMA)
Part Number
Type
Control
Localisation
Quantity
Isotype
6. Hartnell A, et. al., Blood. 97:288-296, 2001.
7. Marmey B, et. al., Hum Pathol. 37:68-77, 2006.
8. Hsiao CH, et. al., J Formos Med Assoc. 105:701-707, 2006.
9. Qubaja M, et. al., Vichows Arch. 454:411-419, 2009.
760-2504 05266904001
Mouse Monoclonal
Hodgkin’s Lymphoma
50 tests
IgM
Hodgkin’s lymphoma, Reed-Sternberg cells
Description
Antibodies against CD15 normally react in a strong cell surface membrane staining pattern with granulocytes and granulocyte precursors,
monocytes, a subset of tissue macrophages and activated T-lymphocytes. CONFIRM CD15 (MMA) Mouse Monoclonal Primary Antibody
also reacts against the Reed-Sternberg cells of Hodgkin’s disease and, because it recognises a carbohydrate antigen, is also expressed by
a variety of carcinoma types, but is negative in malignant mesotheliomas. Antibodies against CD15 are used diagnostically to (1) support
a diagnosis of acute myeloid leukaemia, (2) help support a diagnosis of Hodgkin’s disease and (3) help separate poorly differentiated
adenocarcinomas from malignant mesothelioma.
References
1. Pinkus GS, et al., Am J Pathol. 119:244-252, 1985.
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CD19 NEW
(SP110)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
790-4587 06434681001
Rabbit Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
B-cell acute lymphocytic leukaemia
Description
CD19 (SP110) Rabbit Monoclonal Primary Antibody is directed against the CD19 protein. This antibody may be used to detect the CD19
standard and variant proteins in the vast majority of B cell lymphomas including follicular lymphoma, diffuse large B-cell lymphoma and
Burkitt’s lymphoma.
References
1. Mehra R, et al. Mod Path, 20:538-544, 2007.
2. Tedder T, et al. Immunol Today. 15:437-442, 1994.
3. Scheuermann R, et al. Leuk Lym. 18:385-3977, 1995.
4. Ginaldi L, et al. J Clin Pathol. 51:364-369, 1998.
CD20, CONFIRM
(L26)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Masir N, et al. Histopath. 2006;48:239-246.
6. Kraj M, et al. Leuk Res. 35:169-176, 2011.
7. Hultin LE, et al. Cytometry. 14:196–204, 1993.
8. Foss H, et al. Blood. 94:3108-31013, 1999.
760-2531 05267099001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgG2a/K
Tonsil
Description
CONFIRM CD20 (L26) Mouse Monoclonal Primary Antibody specifically binds to antigens located in the plasma membrane and
cytoplasmic regions of normal B lymphocytes and may also be expressed in Reed-Sternberg cells. This phosphoprotein is found on
the surface of normal and malignant B cells and is thought to act as a receptor during B cell activation and differentiation. Plasma cells
are negative for CD20. CD20 (L26) antibody can be used to detect B cells from peripheral blood, lymph node, spleen, tonsil and bone
marrow. It is also used in the classification of a majority of B cell malignancies, including many acute leukaemias and chronic lymphocytic
leukaemias.
References
1. Coles FB, et al., Mod Pathol. 1(4): 274-278, 1988.
HOME
D21
C
(2G9)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4245 05269059001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgG2a
Tonsil, germinal centre
Description
CD21 antigen is an integral membrane glycoprotein of molecular weight 145 kD. CD21 (2G9) Mouse Monoclonal Primary Antibody is useful
in the identification of follicular dendriditic cell matrix found in normal lymph nodes and tonsillar tissue. This antibody also labels follicular
dendritic cell tumour/sarcomas. The antigen is absent on T-lymphocytes, monocytes and granulocytes.
References
1. Dillon KM, et al., J Clin Pathol. 55(10):791-4, 2002.
2. Pileri SA, et al., Histopathology. 41:1-29, 2002.
3. Kunihiko M, et al., J Histochem Cytochem. 50:1475-1485, 2002.
4. Herrmann LM, et al., Am J Pathol. 162:1075-1081, 2003.
CD21
(EP3093)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Biddle DA, et al., Modern Pathology. 15:50-58, 2002.
6. Cheuk W, et al., Am J Surg Pathol. 25(6):721-31, 2001.
7. Chang KC, et al., J Pathol. 201(3):404-12, 2003.
8. Chan AC, et al., Histopathol. 38(6):510-8, 2001.
760-4438 05973902001
Rabbit Monoclonal
Lymph Node, Tonsil
50 tests
IgG1
Tonsillar hypertrophy
Description
CD21 antigen is an integral membrane glycoprotein of molecular weight 140 kD. CD21 (EP3093) Rabbit Monoclonal Primary Antibody
is useful in the identification of follicular dendritic cell matrix found in normal lymph nodes and tonsillar tissue. This antibody also labels
follicular dendritic cell tumour/sarcomas. The antigen is absent on T-lymphocytes, monocytes and granulocytes.
References
1. Dillon KM, et al. J Clin Pathol. 55(10):791-4, 2002.
2. Pileri SA, et al. Histopathology. 41(1):1-29, 2002.
3. Maeda K, et al. J Histochem Cytochem. 50(11):1475-1485, 2002.
4. Herrmann LM, et al. Am J Pathol. 162(4):1075-1081, 2003.
5. Biddle DA, et al. Modern Pathology. 15(1):50-58, 2002. Erratum
in: Mod Pathol. 15(4):475, 2002.
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CD22 NEW
(SP104)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
790-4588 06391117001
Rabbit Monoclonal
Tonsil, Lymphoma
50 tests
Tonsil
Description
CD22 (SP104) Rabbit Monoclonal Primary Antibody is directed against the type 1 integral membrane glycoprotein CD22. CD22 (SP104)
antibody exhibits a cell membranous and/or cytoplasmic staining pattern, and may be used to aid in the identification of B-cell lymphomas.
References
1. Townsend MJ, et al. Immunol Rev. 237:264-283, 2010.
2. Poe JC, et al. Nature Immunol. 5:1078- 1087, 2004.
3. Tedder TF, et al. Annu Rev Immunol. 15:481-504, 1997.
CD23, CONFIRM
(SP23)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Polson Ag, et al. Leukaemia. 24:1566-1573, 2010.
5. Boue DR, et al. Blood. 71:1480-1486, 1988.
790-4408 05479258001
Rabbit Monoclonal
Tonsil
Cytoplasmic
50 tests
IgG1
Normal tonsil
Description
Expression of CD23 has been detected in neoplastic cells from cases of B-cell chronic lymphocytic leukaemias and B-cell small
lymphocytic lymphomas. Some hairy cell leukaemias and diffuse large B-cell lymphomas are likewise positive. CD23 is generally absent on
mantle cell lymphomas. Accordingly, CD23 staining may be used in the classification of small lymphocytic lymphomas (usually positive) and
mantle cell lymphomas (usually negative).
References
1. Kaiserlian D, et. al., Immunology. 80:90-95, 1993.
2. Aubry JP, et al., The CD23 antigen is the human lymphocyte
receptor for IgE. In:McMichael AJ, Beverley PCL, Cobbold S,
Crumpton MJ, Gilks W, Gotch FM, et al. Leukocyte Typing III, White
Cell Differentiation Antigens. Oxford University Press, Oxford, 1987:
417-419.
3. Kikutani H, et al., CD23 is a low affinity Fcε receptor (FcεR):
regulation of FcεR expression. In: McMichael AJ, Beverley PCL,
Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al. Leukocyte Typing
III, White Cell Differentiation Antigens. Oxford University Press, Oxford,
1987: 419-422.
4. Pallesen G. The distribution of CD23 in normal human tissues
and in malignant lymphomas. In: McMichael AJ, Beverley PCL,
Cobbold S, Crumpton MJ, Gilks W, Gotch FM, et al. Leukocyte
Typing III, White Cell Differentiation Antigens. Oxford University
Press, Oxford, 1987: 383-386.
HOME
CD25
(4C9)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4439 05973899001
Mouse Monoclonal
Lesions of Mastocytosis, Small Bowel
50 tests
IgG2b
Spleen, hairy cell leukaemia
Description
According to the World Health Organisation classification system, the major diagnostic criterion for bone marrow involvement by
systemic mastocytosis (SM) is the presence of dense aggregates (>15 cells) of mast cells. Expression of CD25, a low-affinity receptor
for interleukin-2 (IL-2), is a reliable diagnostic tool for distinguishing neoplastic mast cell aggregates from reactive proliferations and has
therefore recently become a minor criterion for the diagnosis of SM. Hahn, et al. demonstrated that aberrant staining of mast cell clusters
by CD25 antibody in GI (gastro-intestinal) biopsies was essentially diagnostic of SM. CD25 antibody has also been useful in identifying
mast cells in skin biopsies in the setting of urticaria pigmentosa, which is predictive of SM. Quantitation of regulatory T cells (Treg) in the
setting of hepatocellular carcinoma (HCC) has been used as an independent predictive factor of tumour recurrence after hepatic resection
for HCC. Also, the percentage of tumour infiltrating CD25 + FOXP3 + regulatory T cells among tumour cells, inside tumour parenchyma
and at its periphery, is significantly higher in recurrent cutaneous melanoma than in non-recurrent melanoma.
References
1. Hahn HP, et al., Am J Surg Pathol. 31(11):1669-76, 2007.
2. Hollmann TJ, et al., Am J Surg Pathol. 32(1):139-45, 2008.
3. Miracco C, et al., Oncol Rep. 18(5):1115-22, 2007.
CD30, CONFIRM
(Ber-H2)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Siddiqui SA, et al., Clin Cancer Res. 13(7):2075-81, 2007.
5. Yang XH, et al., J Hepatol. 45(2):254-62, 2006.
790-2926 05278201001
Mouse Monoclonal
Hodgkin’s Lymphoma
Membranous
50 tests
IgG1/K
Description
CD30 antigen is expressed in mononuclear Hodgkin’s cells and multinucleated Reed Sternberg cells of Hodgkin’s lymphoma, as well as on
anaplastic large cell lymphomas. This antibody variably produces membranous, cytoplasmic and Golgi staining of both lymphoma cells and
scattered large activated B and T cells in lymph nodes, spleen, tonsil and thymus. This antibody may also stain a small proportion of plasma
cells.
References
1. Froese P, et al., J Immunology. 139(6): 2081-2087, 1987.
2. Stein H, et al., Blood. 66: 848-858, 1985.
3. Stein H, et al., Int J Cancer. 30(4): 445-459, 1982.
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CD31
(1A10)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4246 05269067001
Mouse Monoclonal
Appendix, Placenta, Tonsil
50 tests
IgG1/K
Tonsil
Description
CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental
studies as well as for bone marrow transplantation. Endothelial cells also express this marker, therefore, antibodies to CD31 have been
used as a tool to identify the vascular origin of neoplasms. CD31 has shown to be highly specific and sensitive for vascular endothelial cells.
Staining of non-vascular tumours (excluding hematopoietic neoplasms) has not been observed.
References
1. Parums DV, et al., J. Clin. Path. 1990; 43:752-757.
2. De Young BR, et al., Ap. Immuno. 1993; 1:97-100.
3. Alles JU, et al., J. Histo. Cyto. 1986; 34:209-214.
CD31
(JC-70)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Santeusanio G, et al., Appl. Immunohistochem Mol. Morphol.
2003 Dec; 11(4):359-63.
5. Alexander-Sefre F et al., J. Clin. Pathol. 2003 Oct; 56(10):786-8.
6. Allison KH et al., Am. J. Surg. Pathol. 2004 Mar; 28(3):298-307.
760-4378 05463475001
Mouse Monoclonal
50 tests
IgG1/K
Tonsil
Description
CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental
studies as well as for bone marrow transplantation. Endothelial cells also express this marker, therefore, antibodies to CD31 have been
used as a tool to identify the vascular origin of neoplasms. CD31 has shown to be highly specific and sensitive for vascular endothelial cells.
Staining of non-vascular tumours (excluding hematopoietic neoplasms) has not been observed.
References
1. Parums DV, et al., J Clin Pathol. 43(9):752-7, 1990.
3. Alles JU, et al., J Histochem Cytochem. 34(2):209-14, 1986.
2. De Young BR, et al., Applied Immunohistochemistry. 1: 97-100, 1993. 4. Alexander-Sefre F, et al., J Clin Pathol. 56(10):786-8, 2003.
HOME
CD34
(QBEnd/10)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-2620 05267323001
Mouse Monoclonal
Membranous
50 tests
IgG1
Breast carcinoma
Description
CD34 (QBEnd/10) Mouse Monoclonal Primary Antibody recognises a cell surface antigen of approximately 110 kD that is expressed
selectively on human hematopoietic progenitor cells, including myeloid and lymphoid lineage progenitors, and a significant portion of
acute leukaemia. In addition to this stem cell recognition, this marker is expressed by vascular endothelium. Additionally, it appears that
proliferating endothelial cells express this molecule in greater amounts than non-proliferating endothelial cells.
References
1. Civin CL, et al., London Academic Press. 818-825, 1989.
2. Fina L, et al., Blood. 75:2417-2426, 1990.
3. Sankey EA, et al., J Pathol. 43:752-757, 1990.
4. Ramani P, et al., Histopathology, 17:237-242, 1990.
CD43
(L60)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Aziza J, et al., Am J Clin Pathol. 96:25-31, 1990.
6. Torlakovic G, et al., Arch Pathol Lab Med. 126(7):823-8, 2002.
7. Salizzoni M, et al., Transplantation. 15;76(5):844-8, 2003.
8. Fanburg-Smith JC, et al., Mod Pathol. 16(3):263-71, 2003.
760-2511 05266980001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgG1
Tonsil, cytoplasmic membrane staining of T-lymphocytes
Description
CD43 (also known as leucosialin or sialophorin) is a glycoprotein antigen expressed on the membrane and in the cytoplasm of T cells, cells
of myeloid origin and some B cells. CD43 (L60) Mouse Monoclonal Primary Antibody binds specifically to antigens located in the plasma
membrane and cytoplasmic regions of normal granulocytes, monocytes, histiocytes, T cells and some B cells. Tumours expressing this
antigen include most T-cell lymphomas and some B-cell lymphomas.
References
1. Strickler JG, et al., Hum Pathol. 18(8):808-14, 1987.
2. Stross WP, et al., J Clin Pathol. 42(9):953-61, 1989.
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CD44 NEW
(SP37)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
790-4537 06364985001
Rabbit Monoclonal
Tonsil
Membranous
50 tests
IgG
Description
CD44 (SP37) Rabbit Monoclonal Primary Antibody is directed against the CD44 protein. CD44 is a type I transmembrane receptor protein
expressed in several cell types including T and B lymphocytes, monocytes, granulocytes, erythrocytes, fibroblasts, epithelial and mast
cells. This antibody exhibits a membranous staining pattern and may be used to detect the CD44 standard and variant proteins in a variety
of neoplastic tissues, including squamous cell and urothelial carcinoma.
References
1. Aruffo A, et al., Cell. 61:1303-13,1990.
2. Hascall V, et al., Cold Spring Harbor Laboratory Press. 2008.
3. Endo K, et al., J. of Hepatology. 32:78-84, 2000.
4. Southgate J, et al., Int. J. Cancer. 62:449-456, 1995.
5. Bartolazzi A, et al., JCB. 132(6):1199-1208, 1996.
CD45 (LCA), CONFIRM
(RP2/18)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2505 05266912001
Mouse Monoclonal
Tonsil
Membranous
50 tests
IgG1/K
Lymphoma
Description
CD45, LCA (leucocyte common antigen) is expressed on the cytoplasmic membrane of T and B lymphocytes, monocytes, macrophages,
mast cells and weakly on granulocytes. CONFIRM CD45, LCA (RP2/18) Mouse Monoclonal Primary Antibody is directed against the
CD45RB epitope found on the membrane of leucocytic cells. This antibody has been shown to react with the 220-, 205- and 190 kD
isoforms of CD45.
References
1. Zapata JM, et al., J Immunol. 152(8): 3852-3861, 1994.
2. Pulido R, et al., J Immunol. 140(11): 3851-3857, 1988.
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CD45 (LCA)
(2B11 & PD7/26)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4279 05269423001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgG1/K
Tonsil
Description
Leucocyte Common Antigen (LCA) Mouse Monoclonal Primary Antibody is routinely used to aid in the differential diagnosis of
undifferentiated neoplasms, whenever malignant lymphoma is suspected by the morphological or clinical data. It is a highly specific
antibody therefore a positive result is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack
CD45 (Hodgkin’s disease, some T-cell lymphomas and some leukaemias) so its absence does not rule out a hematolymphoid tumour. This
antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes,
erythrocytes and plasma cell precursors.
References
1. Mason DY, et al., Am J Pathol. 128:1-4, 1987.
2. Hall PA, et al., Histopathology. 13:149-160, 1988.
3. Kurtin PJ, et al., Hum Path. 16:353-365, 1985.
4. Maluf HM, et al., Mod Pathol. 8(2): 155-9, 1995.
CD45R
(MB1)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Caballero T, et al., J Clin Pathol. 48(8): 743-8, 1995.
6. Vasef MA, et al., Am J Clin Pathol. 108(1): 54-9, 1997.
7. Saxena A, et al., Am J Clin Pathol. 112(4): 495-512, 1999.
8. Kraus MD, et al., Am J Surg Pathol. (8): 1068-78, 2000.
760-2624 05267340001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgG1
Large B-cell lymphoma
Description
CD45R antibody stains preferentially B cells and their neoplasms, but is less specific as it will also react with some T-cell lymphomas and
non-lymphoid tumours. The antigen for this antibody is in the membrane of all B cells with the exception of plasma cells and some mature
T cells.
References
1. Hall PA, et al., J Clinical Pathology. 40:870-873, 1987.
2. Myskow MW, et al., Am J Pathology. 90:564-574, 1988.
3. West KP, et al., J Pathology. 150:89-101, 1986.
4. Poppema S, et al., Am J Pathology. 127:418-429, 1987.
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CD45RO, CONFIRM
(UCHL-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
790-2930 05278244001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgG2a
Lymph node, normal
Description
CONFIRM CD45RO (UCHL-1) Mouse Monoclonal Primary Antibody reacts predominantly with the p180 component of the Leucocyte
Common Antigen (LCA) antigen family and weakly with the 190 kD and 205 kD isoforms. This reagent reacts with most T-lymphocytes,
macrophages and Langerhan’s cells of normal tissue.
References
1. Berti E, et al., Am J Clin Pathol. 95(2):188-193, 1991.
CD56 NEW
(MRQ-42)
Part Number
Type
Control
Localisation
Quantity
760-4596 06433359001
Rabbit Monoclonal
Pancreatic Islet Cells, Pancreatic Endocrine Cells, Neuroblastoma
50 tests
Pancreatic neuroendocrine carcinoma
Description
CD56 (MRQ-42) Rabbit Monoclonal Primary Antibody is used as an aid in the identification of neural/neurodendocrine tissues, natural
killer (NK) cells, NK-like T-cells and diagnosis of related neoplasms within the context of an antibody panel, the patient’s clinical history
and other diagnostic tests evaluated by a qualified pathologist.
References
1. Gerardy-Schahn, R et al. Int J Canc Suppl 8:38- 42, 1994.
2. Michalides, R et al. Int J Canc Suppl 8:34-37, 1994.
3. Kibbelaar, RE et al. Euro J of Cancer. 27(4):431-435, 1991.
4. Langdon, SP et al. Cancer Research. 48(21):6161-6165, 1988.
5. Sumi, M et al. Leuk Lymphoma. 44(1): 201-4, 2003.
6. Trejo, O et al. J Cutan Pathol. 29(7): 397-406, 2002.
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CD57
(NK-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-2626 05267366001
Mouse Monoclonal
Tonsil, Lymph Node
Membranous
50 tests
IgM/K
Tonsil
Description
CD57 (NK-1) Mouse Monoclonal Primary Antibody marks a subset of lymphocytes known as natural killer (NK) cells. Follicular centre cell
lymphomas often contain many NK cells within the neoplastic follicles. NK-1 also stains neuroendocrine cells and their derived tumours,
including carcinoid tumour and medulloblastoma. NK-1 reportedly also reacts with a variety of cell types in non-lymphoid tissues.
References
1. Lanier LL, et al., Journ Immun. 131(4):1789-1796, 1983.
2. Ritchie AW, et al., Clin and Exp Imm. 51(3):439-447, 1983.
3. Caillaud JM, et al., Cancer Res. 44(10):4432-4439, 1984.
CD61
(2f2)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Tucker GC, et al., Cell Differentiation. 14(3):223-230, 1984.
5. Abo T, et al., Cellular Immun. 73(2):376-384, 1982.
760-4249 05269091001
Mouse Monoclonal
Bone Marrow
Cytoplasmic
50 tests
IgG1
Bone marrow
Description
CD61 (2f2) Mouse Monocloanl Primary Antibody labels the IIIa subunit of the non-covalently linked glycoprotein heterodimer IIb/IIIa
complex present on human platelets and their precursors. This antibody is useful in identifying megakaryoblastic differentiation as seen in
megakaryoblastic leukaemia.
References
1. Thiele J, et al., Eur J Haematol. 44:63-70, 1990.
2. Thiele J, et al., Virchows Archiv B Cell Pathol. 58:295-302, 1990.
3. Goldman BI, et al., Modern Pathology. 14:589-594, 2001.
4. Fox SB, et al., Histopathology. 17(1):69-74, 1990.
5. Duperray A, et al., Blood. 74(5):1603-11, 1989.
6. Campana D, et al., Leukaemia. 4(9):620-4, 1990.
7. Thiele J, et al., Anal Quant Histol. 12(4):285-9, 1990.
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CD63
(NKI/C3)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4379 05463483001
Mouse Monoclonal
Malignant Melanoma
50 tests
IgG1/K
Malignant melanoma in tonsil
Description
This antibody reacts with a 53 kDa protein which forms a part of the family of tetraspan moieties. The antigen was originally designated
as a lysosomal membrane protein characterised as an activation dependent platelet surface antigen. In fact the CD63 antigen has a
diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.
It has been quite useful in identifying malignant melanoma. Mete, et al. found it useful in differentiating renal oncocytomas (RO) from
eosinophilic renal cell carcinomas (eRCC). In his series of 35 ROs, 94% demonstrated apical and/or polar CD63 positivity. Of his 77 eRCCs,
96% showed diffuse cytoplasmic staining.
References
1. Azorsa DO, et al., Blood. 15:78(2):280-4, 1991.
2. Barrio MM, et al., Hybridoma. 17(4):355-64, 1998.
3. Demetrick DJ, et al., J Natl Cancer Inst. 84(6):422-9, 1992.
CD68, CONFIRM
(KP-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Mete O, et al., Virchows Arch. 447(6):938-46, 2005. Epub 2005 Aug 17.
5. Kwon MS, et al., Lung Cancer. 57(1):46-53, 2007. Epub 2007 Mar 12.
790-2931 05278252001
Mouse Monoclonal
Tonsil, Lymph Node
50 tests
IgG1/K
Tonsil, histiocytes
Description
CONFIRM CD68 (KP-1) Mouse Monoclonal Primary Antibody reacts with a 110 kD intracellular glycoprotein associated with the cell
membrane of macrophages and some myeloid elements.
References
4. Pulford KAF, et al., J Clin Pathol. 42:414-21, 1989.
1. Facchetti F, et al., Histopathology. 19:141-5, 1991.
5. Vergier B, et al., Blood. 959(7):2212-8, 2000.
2. Ruco LP, et al., Am J Clin Pathol. 92:273-9, 1989.
3. Cordell JL, et al., Oxford Univ. Press-Oxford, New York 1995; 925-927.
HOME
CD71 NEW
(MRQ-48)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4589 06433162001
Mouse Monoclonal
Bone Marrow
50 tests
IgG1
Description
CD71 (MRQ-48) Mouse Monoclonal Primary Antibody is indicated as an aid in the identification of the erythroid precursors in bone
marrow and in the determination of acute myeloid leukaemia and myelodysplastic syndrome (MDS).
CD79a, CONFIRM
(SP18)
Part Number
Type
Control
Localisation
Quantity
790-4432 05640296001
Rabbit Monoclonal
B Cell Lymphoma, Tonsil
50 tests
Non-Hodgkin’s lymphoma
Description
CONFIRM CD79a (SP18) Rabbit Monoclonal Primary Antibody is intended for the detection of CD79a in normal and neoplastic human
tissues. CD79a antibody is directed against a protein expressed on the surface of B lymphocytes at all stages of maturation, from B
lymphocyte precursors through plasma cells. Expression of CD79a may aid in the identification of B cell lineage in malignant lymphomas.
References
1. Berg JM, et al., Biochemistry, 5th Ed. WH Freeman and Company,
NY, 2002.
2. Chu PG, et al., Appl Immunohistochem Mol Morphol. 9(2):
97-106, 2001.
3. Bhargava P, et al., Am J Clin Pathol. 128(2):306-313, 2007.
4. Blakolmer K, et al., Mod Pathol. 13(7):766-772, 2000.
5. Mason DY, et al. Blood. 86(4):1453-9, 1995.
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CD99, CONFIRM
(O13)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
790-4452 05913594001
Mouse Monoclonal
Pancreatic Islet Cells, Sertoli Cells of the Testis
Membranous
50 tests
IgG1
Ewing’s sarcoma
Description
CONFIRM CD99 (O13) Mouse Monoclonal Primary Antibody is directed against human CD99 expressed on T-lymphocytes, cortical
thymocytes, granulosa cells of the ovary, pancreatic islet cells, CNS ependymal cells and Sertoli’s cells. CD99 shows a plasma
membrane location. This antibody may be used as part of a panel to aid in the identification of Ewing’s sarcoma and related peripheral
neuroectodermal tumours.
References
1. Ambros IM, et al., Cancer. 67:1886-1893, 1991.
2. Scotlandia K, et al., Cancer Research. 60(18):5134-5142, 2000.
3. Milanezi F, et al., Histopathology. 39:578–583, 2001.
CD138/syndecan-1
(B-A38)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Pelosi G, et al., Virchows Arch. 437:270–274, 2000.
5. Gordon MD, et al., Mod Pathol. 11:769–773, 1998.
6. Ozdemirli M, et al., Mod Pathol. 14:1175–1182, 2001.
760-4248 05269083001
Mouse Monoclonal
Tonsil, Plasmacytoma
Membranous
50 tests
IgG
Tonsil, plasma cells
Description
CD138/syndecan-1 (B-A38) Mouse Monoclonal Primary Antibody is a useful marker for labelling normal and neoplastic plasma cells and
plasmacytoid lymphomas. Various forms of Hodgkin’s disease have also shown positive staining with this antibody.
References
1. Chilosi M, et al., Mod Pathol. 12(12):1101-6, 1999.
2. Sebestzen A, et al., Br J Haematol. 104(2):412-9, 1999.
3. Carbone A, et al., Blood. 91(3):747-55, 1998.
4. Bayer-Garner IB, et al., Mod Pathol. 14(10):1052-8, 2001
5. Lampert IA, et al., Appl Immunohistochem Mol Morphol. 13(2):12431, 2005.
6. Oksanen A, et al., J Clin Pathol. 58(4):376-81, 2005.
7. O’Connell FP, et al., Am J Clin Pathol. 121(2):254-63, 2004.
8. Coloma L, et al., Am J Surg Pathol. 28(6):736-47, 2004.
HOME
CD163
(MRQ-26)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4437 05973929001
Mouse Monoclonal
Inflamed Tissue
50 tests
IgG1
Placenta, normal
Description
CD163 (MRQ-26) Mouse Monoclonal Primary Antibody was recently identified as an acute phase-regulated transmembrane protein whose
function is to mediate the endocytosis of haptoglobinhemoglobin complexes. This receptor is expressed on the surface of monocytes (low
expression) and tissue macrophages, [also known as histiocytes] (high expression). It is a member of the cysteine-rich scavenger receptor
superfamily, encoded by a gene localised on human chromosome 12p13.3. Soluble in plasma, CD163 functions as an anti-inflammatory
signal and has many roles in disease processes that range from auto-immune conditions such as rheumatoid arthritis to atherosclerosis.
Previous work has shown that the CD163 gene can be regulated by glucocorticoids, IL-10 and other inflammatory modulators, and is
highly expressed in inflamed tissues, consistent with its role in the resolution of inflammation. Staining for CD163 has been helpful in
distinguishing synovial macrophages from synovial intimal fibroblasts in the setting of rheumatoid arthritis, where its specificity for
macrophages was found to be superior to that of CD68, which does not discriminate between these cell types. Increased levels of CD163
were also detected in patients with microbial infections and myelomonocytic leukaemias by an enzyme-linked immunosorbent assay. Flow
cytometry studies have confirmed that CD163 expression is limited to leukaemias with monocytic differentiation. Another recent study
showed that all 5 cases of synovial-type giant cell tumours of the vertebral column stained for CD163.
References
1. Backe E, et al., J Clin Pathol. 44(11):936-945, 1991.
2. Buechler C, et al., J Leukoc Biol. 67(1):97-103, 2000.
3. Hiraoka A, et al., Pathol Res Pract. 201(5):379-84, 2005.
4. Hogger P, et al., J Immunol. 161(4):1883-1890, 1998.
5. Kristiansen M, et al., Nature. 409(6817):198-201, 2001.
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CDX-2
(EPR2764Y)
Part Number
Type
Control
Localisation
Quantity
Isotype
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760-4380 05463491001
Rabbit Monoclonal
Adenocarcinoma of Colon, Normal Colon
Nuclear
50 tests
IgG
Colon adenoma
Description
CDX-2 is a caudal-related homeobox transcription factor whose expression in the adult is normally restricted to the intestinal epithelium.
It is implicated in the development and maintenance of the intestinal mucosa. This protein is expressed immunohistochemically in the
nuclei of normal intestinal epithelium. Loss of CDX-2 protein expression has been correlated with loss of differentiation in colorectal
cancers. CDX-2 (EPR2764Y) Rabbit Monoclonal Primary Antibody has been useful in distinguishing gastro-intestinal origin of metastatic
adenocarcinomas and carcinoids. A high percentage of mucinous carcinomas of the ovary also stain positively with this antibody as well as
carcinomas from the upper gastro-intestinal tract.
References
1. Levine PH, et al., Diagn Cytopathol. 34(3):191-5, 2006.
2. Mazziotta RM, et al., Appl Immunohistochem Mol Morphol.
13(1):55-60, 2005.
3. Saqi A, et al., Am J Clin Pathol. 123(3):394-404, 2005.
4. Erickson LA, et al., Endocr Pathol. 15(3):247-52, 2004.
Chromogranin A
(LK2H10)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Saad RS, et al., Am J Clin Pathol. 122(3):421-7, 2004.
6. Kaimaktchiev V, et al., Mod Pathol. 17(11):1392-9, 2004.
7. Groisman GM, et al., Int J Gynecol Pathol. 23(1):52-7, 2004.
8. Moskaluk CA, et al., Mod Pathol. 16(9):913-9, 2003.
9. Werling RW, et al., Am J Surg Pathol. 27(3):303-10, 2003.
760-2519 05267056001
Mouse Monoclonal
Pancreas
Cytoplasmic
50 tests
IgG1/K
Pancreatic islet cells
Description
Chromogranin A (LK2H10) Mouse Monoclonal Primary Antibody specifically binds to the chromogranin A protein located in the secretory
granules of normal and neoplastic neuroendocrine cells. Chromogranin A is also expressed by adrenal (medulla), parathyroid, anterior
pituitary cells, islet cells of the pancreas, gastro-intestinal cells, bronchial endocrine cells and thyroid C cells. Chromogranin A is expressed
in tumours of neuroendocrine origin, including pheochromocytomas, pituitary adenomas, islet cell tumours, medullary thyroid carcinomas,
carcinoids and Merkel cell tumours. This antibody may be used to aid in the identification of cells of normal or neoplastic neuroendocrine
lineage.
References
1. Wilson BS, et al., Am J Clin Pathol. 115(3): 458-468, 1984.
As shown, the PATHWAY c-KIT (9.7)
has the characteristic-specific doublet
band of 140-145 kD in lysates from
GIST 822 cells. It does not cross-react
with lysates from homologous receptor
tyrosine kinases PDGFR alpha, c-FMS
or Flt-3.
Collagen Type IV
(CIV22)
Part Number
Type
Control
Localisation
Quantity
Isotype
3
<PREV
Flt-
MS
c -F
Rα
GF
PD
c-K
IT
HOME
760-2632 05267439001
Mouse Monoclonal
Muscle, Lung
Basement Membrane
50 tests
IgG1/K
Lung
Description
Collagen Type IV is the major component of the basal lamina, so antibodies to this molecule confirm its presence and reveal the
morphological appearance of the structure. Normal tissue stains with this antibody in a fashion consistent with the sites of mesenchymal
elements and epithelial basal laminae. Collagen IV (CIV22) Mouse Monoclonal Primary Antibody can also be useful in the classification
of soft tissue tumours; schwanomas, leiomyomas and their well differentiated, malignant counterparts usually immuno react with this
antibody. The vascular nature of neoplasms, hemangiopericytoma, angiosarcoma and epithelioid hemangioendothelioma can be revealed
by this antibody with greater reliability than non-specific stains (e.g. silver reticulum).
References
1. Gould VE, et al., Pathol Annul. 11:353-386, 1976.
2. McArdle JP, et al., Int J Cancer. 34:633-638, 1984.
3. Sakr WA, et al., Hum Path. 18:1043-1050, 1987.
4. Barsky SH, et al., Am J Surg Path. 7:667-677, 1983.
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COX-2 (Cyclooxygenase 2)
(SP21)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4254 05269148001
Rabbit Monoclonal
Colon Adenocarcinoma
Cytoplasmic
50 tests
IgG
Colon adenocarcinoma
Description
Cyclooxygenase 2 (COX-2) catalyses the conversion of arachidonic acid to prostaglandin H2 in the first step in the biosynthesis of
prostaglandins, thromboxanes and prostacyclins. COX-2 inhibition by non-steroidal anti-inflamatory agents has been shown to decrease
angiogenesis and tumour growth and promote apoptosis. COX-2 overexpression has been associated with increased microvascular
density. COX-2 overexpression has also been suggested as a poor prognostic indicator in carcinomas of the colon, breast, pancreas and
adenocarcinoma of the lung.
References
1. Stoehlmacker J, et al., Semin Oncol. (3)suppl 6:10-16, 2003.
2. Gallo O, et al., Hum Pathol. 33(7):708-714, 2002.
3. Sano H, et al., Cancer Res. 55(17):3785-9, 1995.
Cyclin D1 NEW
(SP4-R)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Denkert C, et al., Cancer. 97(12):2978-87, 2003.
5. Sheehan KM, et al., Hum Pathol. 34:1242-1246, 2003.
790-4508 05862949001
Rabbit Monoclonal
Tonsil
Nuclear
50 tests
IgG
Description
Cyclin D1 (SP4-R) Rabbit Monoclonal Primary Antibody is directed against the cyclin D1 molecule, a key cell cycle regulator, expressed
primarily during the G1 phase of the cell cycle. This antibody exhibits a nuclear staining pattern and may be used to aid in the identification
of mantle cell lymphoma and breast carcinoma.
References
1. Milenkovic S, et al., Cell Mol Neurobiol. 28:907-913, 2008.
2. Yang K, et al., Cell Division. 1:1-8, 2006.
3. Lesage D, et al., Int J Cancer. 115:171-176, 2005.
4. Arnold A, et al. J Clin Oncol. 23:4215-4224, 2005.
5. Dobashi Y, et al., Int J Cancer. 110:532-541, 2004.
6. Yang C, et al., Urologia Internationalis. 69:190-194, 2002.
7. Aguilera NS, et al., Am J of Path. 153:1969-76, 1998
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<PREV
Cytokeratin, CONFIRM
(34βE12)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4373 05479266001
Mouse Monoclonal
Prostate
Cytoplasmic
50 tests
IgG1
Prostate carcinoma
Description
CONFIRM Cytokeratin (34βE12) Mouse Monoclonal Primary Antibody recognises cytokeratins 1, 5, 10 and 14 of the Moll catalogue and
is used as an aid in the identification of basal cells in prostate and myoepithelial cells in breast. Clone 34βE12 has been characterised as
identifying high molecular weight (HMW) cytokeratins found in squamous and ductal epithelium over a wide range of organ tissues. Clone
34βE12 reacts with basal cells in normal epithelia of the prostate.
References
1. Gown A, et al., Amer J Clin Pathol. 84: 413-424, 1985.
2. Brawer M, et al., Canc Res. 45: 3663-3667, 1985.
3. Moll R, et al., Cell. 31: 11-24, 1982.
(AE1)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2521 05267064001
Mouse Monoclonal
Prostate, Salivary Gland
Cytoplasmic
50 tests
IgG1
Prostate
Description
CONFIRM Cytokeratin (AE1) Mouse Monoclonal Primary Antibody may be used to aid in the identification of cells of normal and
abnormal epithelial lineage as an aid in diagnosis of anaplastic tumours. Cytokeratin (AE1) recognises an epitope found on human callus
cytokeratins; it reacts with the 56.5 kD, 50 kD, 50’ kD, 48 kD and 40 kD cytokeratins of the acidic subfamily. Because specific combinations
of cytokeratin peptides are associated with different epithelial cells, these peptides are clinically important markers for classifying
carcinomas (tumours of epithelial origin) and for distinguishing carcinomas from malignant tumours of non-epithelial origin such as
lymphomas, melanomas and sarcomas.
References
1. Robbins SL, et al., Pathol Basis of Disease 3rd Ed. W.B. Saunders,
New York, 1984.
2. Woodcock-Mitchell J, et al., J Cell Biol. 95(2 Pt 1): 580-588, 1982.
3. Spagnolo DV, et al., Am J Clin Pathol. 84(6): 697-704, 1985.
4. Thomas P, et al., Hum Pathol. 18(7): 728-734, 1987.
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(AE3)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-2909 05278040001
Mouse Monoclonal
Prostate, Salivary Gland, Bladder
Cytoplasmic
50 tests
IgG1
Stratified squamous epithelium
Description
CONFIRM Keratin (AE3) Mouse Monoclonal Primary Antibody recognises basic cytokeratins of 65-67, 64, 59, 56 and 52 kD. The antibody is
a marker used to distinguish carcinomas from non-epithelial tumours.
References
1. Pinkus GS, et al., J. Histochem and Cytochem. 33(5): 465-473, 1985.
2. Weiss RA, et al., J Cell Biol. 98(4): 1397-1406, 1984.
Cytokeratin NEW
(CAM 5.2)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Tseng SC, et al., Cell. 30(2): 361-372, 1982.
4. Woodcock-Mitchell J, et al., J. Cell Biol. 95(2 pt 1):580-588, 1982.
790-4555 06478425001
Mouse Monoclonal
Pancreatic Carcinoma and Colon
Cytoplasmic
50 tests
IgG
Colon adenocarcinoma
Description
Cytokeratin (CAM 5.2) Mouse Monoclonal Primary Antibody is directed against cytokeratin 7 and cytokeratin 8 present on secretory epithelia
but not on stratified squamous epithelia. This antibody may be used to aid in the identification of tumours of epithelial origin such as
colorectal, pancreatic and non-small cell lung cancer, and in distinguishing carcinomas from other malignant tumours of non-epithelial origin.
References
1. Makin CA, et al. J Clin Pathol. 37:975-983, 1984.
2. Chu PG, et al. Endocr Pathol. 20:1-10, 2009.
3. Quinlan RA, et al. Annals New York Academy of Sciences. 282306, 1985.
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Cytokeratin (Pan)
(AE1/AE3 & PCK26)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-2595 05267145001 (50 tests);
760-2135 05266840001 (250 tests)
Mouse Monoclonal
Intestine, Liver
Cytoplasmic
50 Tests (760-2595), 250 Tests (760-2135)
IgG1
Skin
Description
Pan Cytokeratin (AE1/AE3/PCK26) Mouse Monoclonal Primary Antibody may be used to aid in the identification of normal and abnormal
epithelial cells and to determine the lineage of poorly differentiated malignant tumours. This antibody cocktail recognises most of the
acidic and all of the basic cytokeratins, making it a useful stain for nearly all epithelial tissues and their tumours. Anti-Pan Keratin (AE1/
AE3/PCK26) specifically binds to antigens located in the cytoplasm of simple and complex epithelial cells; it reacts with the 56.5kD, 50kD,
50’kD, 48kD and 40kD cytokeratins of the acidic subfamily and 65-67kD, 64kD, 59kD, 58kD, 56kD and 52kD cytokeratins of the basic
subfamily.
References
1. Woodkcock-Mitchell J, et al., J Cell Biol. 95(2 pt 1): 580-588, 1982.
2. Spagnolo DV, et al., Am J Clin Pathol. 84(6):,697-704, 1985.
3. Lane EB, et al., Nature. 356(6366): 244-246, 1992.
4. Sun TT, et al., Annals NY Acad Sci. 455: 307-329, 1985.
5. Eichner R, et al., Cell Biol. 98(4): 1388-1396, 1984.
Cytokeratin 5/6 NEW
(D5/16B4)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4554 06478441001
Mouse Monoclonal
Prostate and Tonsil
Cytoplasmic
50 tests
IgG1
Lung squamous cell carcinoma
Description
Cytokeratin 5/6 (D5/16B4) Mouse Monoclonal Primary Antibody detects cytokeratins 5 and 6. Cytokeratin 5 is expressed in various
epithelia and mesothelial cells, while cytokeratin 6 is expressed by proliferating squamous epithelium. This antibody exhibits a cytoplasmic
staining pattern and may be used to aid in the identification of squamous cell carcinoma and in distinguishing malignant mesothelioma
from lung adenocarcinomas. The detection of cytokeratin 5 in myoepithelial cells with this antibody may also be used to aid in the
determination of breast and prostate malignancies.
References
1. Pu RT, et al. Diagn Cytopathol. 36:20-25. 2008.
2. Moll R, et al. Cell. 31:11-24, 1982.
3. Clover J, et al. AntiHistopathol. 31:140-143, 1997.
4. Cury PM, et al. Mod. Pathol. 13:107-112, 2000.
5. Otterbach F, et al. Histopathol. 37:232-240, 2000.
6. Trokov K, et al. Am J Clin Pathol. 132:211-220, 2009.
7. Chu PG, et al. Mod Pathol. 15:6-10, 2002.
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Cytokeratin 7, CONFIRM
(SP52)
Part Number
Type
Control
Localisation
Quantity
790-4462 05986818001
Rabbit Monoclonal
Glandular and Ductal Epithelium of Lung, Salivary Gland, Breast Tissue
Cytoplasmic
50 tests
Lung adenocarcinoma
Description
CONFIRM Cytokeratin 7 (SP52) Rabbit Monoclonal Primary Antibody is directed against a type II cytokeratin protein expressed by most
ductal, glandular and transitional epithelia. Cytokeratin 7 exhibits a cytoplasmic localisation. This antibody may be used to aid in the
identification of normal and neoplastic cells of ovary, lung and breast epithelial origin which express Cytokeratin 7, and those of colonic
and prostate epithelial lineage which lack Cytokeratin 7 production.
References
1. Wick MR, et al., Immunohistology of the Gastrointestinal
Tract, Pancreas, Bile Ducts, Gallbladder and Liver. Diagnostic
Immunohistochemistry, 2nd Edition. Philadelphia, PA. Elsevier Inc;
2006:464-465.
2. Rubin BP, et al., Euro J of Cancer Prevent. 10:77-82, 2001.
3. Wang HL, et al., Am J Surg Path. 25 (11):1380-1387, 2001.
Cytokeratin 8
(35betaH11)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Inamura K., et al., Am J Surg Pathol. 29:660-665, 2005.
5. Rekhi B, et al., Diagn Pathol. 3:39-45, 2008.
6. Rossi G, et al., J Thorac Oncol. 2:115–120, 2007.
7. Sack MJ, et al., Diagn Cytopathol. 16:132-136, 1997.
8. Tsao SC, et al., Kaohsiung J Med Sci. 23:325–331, 2007.
760-2637 05267471001
Mouse Monoclonal
Prostate
Cytoplasmic
50 tests
IgM/K
Andenocarcinoma
Description
Cytokeratin 8 is reactive with the majority of epithelium and epithelial tumours; non-squamous tumours stain positive and squamous
tumours stain negative. This antibody stains positive for adenocarcinomas of the breast, ovary, gastro-intestinal tract, thyroid, pancreas,
bile duct and salivary glands. This antibody does not react with skeletal muscle or nerve cells.
References
1. Gown AM, et al., Am J Pathol. 114:309, 1984.
2. O’Malley FP, et al., Virch Arch. A. 417:191, 1990.
3. Mahul B, et al., Arch Pathol Lab Med. Vol. 118:260-264, 1994.
4. Wojno KJ, et al., Am J Surg Pathol. 19(3):251-60, 1995.
HOME
Cytokeratin 8 & 18
(B22.1 & B23.1)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4344 05269776001
Mouse Monoclonal
Pancreas, Prostate, Salivary Gland
Cytoplasmic
50 tests
IgG1
Breast
Description
Cytokeratin 8 & 18 (B22.1 & B23.1) Mouse Monoclonal Primary Antibody is a cocktail of two mouse monoclonal antibodies. Cytokeratins
8 & 18 can be found in most simple epithelium, e.g. thyroid, female breast, gastro-intestinal tract and respiratory tract. Adenocarcinomas
and most non-keratinising squamous carcinomas will stain, but keratinising squamous carcinomas will not. This cocktail is used when
attempting to demonstrate the presence of Paget cells; there is very little keratin 18 in the normal epidermis so this will only stain Paget
cells. This approach facilitates the interpretation using immunostains and is more sensitive than mucin histochemistry.
References
1. Angus B, et al., Journal of Pathology. 155:377-384, 1987.
2. Corson JM., Pathol Annual 21 (part 2). 47-81, 1986.
Cytokeratin 10 NEW
(SP99)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Sasaki M et al., Histopathology. 32(3):199-208, 1998.
790-4562 06425135001
Rabbit Monoclonal
Skin
Cytoplasmic
50 tests
Rabbit Ig
Rectal squamous cell carcinoma
Description
Cytokeratin 10 (SP99) Rabbit Monoclonal Primary Antibody is directed against the 56.5kD member of the type 1 cytokeratin family,
cytokeratin 10. Cytokeratin 10 is expressed in keratinising stratified epithelia, but is absent from the basal layer, in various organs including
the skin. Cytokeratin 10 (SP99) antibody exhibits a cytoplasmic staining pattern and may be used to aid in the identification of welldifferentiated squamous carcinomas of the cervix and anus.
References
1. Moll R, et al. Cell. 31:11-24, 1982.
2. Fillies T, et. al. BMC Can. 6:1186-1194, 2006.
3. Parks JM, et al. Human Path. 41:431-437, 2010.
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Cytokeratin 14
(LL002)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4251 05269113001
Mouse Monoclonal
Squamous Mucosa, Squamous Carcinoma
Cytoplasmic
50 tests
IgG3
Squamous mucosa
Description
Cytokeratin 14 is a 50 kD polypeptide found in basal cell of squamous epithelia, some glandular epithelia, myoepithelium and mesothelial
cells. Cytokeratin 14 (LL002) Mouse Monoclonal Primary Antibody has been demonstrated to be useful in differentiating squamous cell
carcinomas from other epithelial tumours. This antibody has also been useful in separating oncocytic tumours of the kidney from its renal
mimics, as well as in determining metaplastic carcinomas of the breast.
References
1. Reis-Filho JS, et al., Appl Immunohistochem Mol Morphol.
11(1):1-8, 2003.
2. Chu PG, et al., Histopathology. 39(1):9-16, 2001.
Cytokeratin 17 NEW
(SP95)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Chu PG, et al., Histopathology. 39(5):455-62, 2001.
4. Dabbs DJ, et al., Diagnostic Immunohistochemistry, ChurchillLivingstone, Philadelphia, 2002; 166-176.
790-4560 06373658001
Rabbit Monoclonal
Cervical Carcinoma, Normal Skin
50 tests
Rabbit Ig
Rectal squamous cell carcinoma
Description
Cytokeratin 17 (SP95) Rabbit Monoclonal Primary Antibody is directed against Cytokeratin 17 expressed in basal cells of complex epithelia,
glandular epithelium with myoepithelial component and transitional and pseudostratified epithelia. This antibody may be used to aid in the
identification of squamous cell carcinomas in various tissues including the cervix, lung and oral cavity.
References
1. Troyanovsky S, et al. J Cell Sci. 93:419-426, 1989.
2. Quinlan R, et al. Ann NY Acad Sci. 455:282-306, 1985.
3. Martens J, et al. Anticancer Res. 24:771-776, 2004.
4. Toyoshima T, et al. J Cancer Res. Clin. Oncol. 134:515-521, 2008.
5. Blobel G, Moll, et al. Cell Pathol. 45:407-429, 1984.
6. Rijn M, et al. Am J Pathol. 161,6:1991-1996, 2002.
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Cytokeratin 19
(A53-B/A2.26)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4281 05269440001
Mouse Monoclonal
Colon and Thyroid Carcinoma
Cytoplasmic
50 tests
IgG1
Colon carcinoma
Description
Cytokeratin 19 (A53-B/A2.26) Mouse Monoclonal Primary Antibody reacts with a wide variety of epithelium and epithelial malignancies
including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver and breast. Perhaps the most useful application is the
identification of thyroid carcinoma of the papillary type, although, follicular carcinoma is also labelled by this antibody approximately 5060% of the time.
References
1. Zubair W, et al., Hum Pathol. 30:1166-1171, 1999.
2. Judkins AR, et al., Hum Pathol. 30:1373-1376, 1999.
3. Cerilli LA, et al., Am J Clin Pathol. 118:186-193, 2002.
4. Nasser SM, et al., Cancer (Cancer Cytopathol). 90:307-11, 2000.
Cytokeratin 20, CONFIRM
(SP33)
Part Number
Type
Control
Localisation
Quantity
790-4431 05587760001
Rabbit Monoclonal
Cytoplasmic
50 tests
Colon carcinoma
Description
CONFIRM Cytokeratin 20 (SP33) Rabbit Monoclonal Primary Antibody is directed against a type I cytokeratin protein of mature
enterocytes and goblet cells of the gastric and intestinal mucosa. This antibody produces a cytoplasmic staining pattern and may be used
to aid in the identification of normal cytokeratin 20 expressing cells and neoplastic cells of colonic epithelial lineage.
References
1. Zhou Q, et al., Mol Biology of the Cell. 14(7):2959-71, 2003.
2. Moll R, et al., Differentiation. 53(2):75-93, 1993.
3. Moll R, et al., Am J of Path. 140(2):427-447, 1992.
4. Chan J, et al., Am J of Surg Path. 21(2):226-234, 1997.
5. Zhou Q, et al., J of Biol Chem. 281(24):16453-16461, 2006.
6. Kyung-Joon M, et al., Path Res and Practice. 202(7):531-535,
2006.
7. Tot T, Annals of Diag Path. 3(6):350-356, 1999.
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Desmin, CONFIRM
(DE-R-11)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-2513 05267005001
Mouse Monoclonal
Intestine
Cytoplasmic
50 tests
IgG1/K
Rhabdomyosarcoma
Description
CONFIRM Desmin (DE-R-11) Mouse Monoclonal Primary Antibody identifies normal muscle cells and myogenic neoplasms bearing the
desmin antigen. The most obvious application of desmin antibodies is in the characterisation of myogenic neoplasms or in the recognition
of myogenic cell lines in mixed sarcomas. Among neoplasms, carcinomas do not react with desmin antibodies, leiomyosarcomas and
rhabdomyosarcomas are positive and other sarcomas (with some exceptions) are negative. Myogenic sarcomas can be classified as such
due to their positive staining with antibodies to muscle (specific) actin and desmin. Because of the different sensitivities and specificities of
these antibodies, they are best used in combination.
References
1. Debus E, et al., EMBO J. 2(12): 2305-2312, 1983.
2. True LD, Atlas of Diag Immuno Histopathol Lippincott Co
Philadelphia, 1990.
DOG1 NEW
(SP31)
Part Number
Type
Control
Localisation
Quantity
3. Taylor RT, et al., Immunomicroscopy 2nd Edition, WB Saunders
Co., Philadelphia, 1994.
760-4590 06433109001
Rabbit Monoclonal
GIST
50 tests
GIST
Description
DOG1 (SP31) Rabbit Monoclonal Primary Antibody is used as an aid in the identification and diagnosis of gastro-intestinal stromal tumours
(GIST) within the context of an antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
References
1. Espinosa, I et al. Am J Surg Pathol. 32(2):210-8, 2008.
2. Miwa, S et al. Mutation. J Gastroen-terol. 43(7):531-7, 2008.
3. Parfitt, JR et al. Gastrointestinal. Histopathology. 52(7):816-23, 2008.
4. West, RB et al. Am J Pathol. 165(1):107-13, 2004.
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E-Cadherin
(36)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
790-4497 05905290001
Mouse Monoclonal
Ductal Breast Carcinoma
Membranous
50 tests
IgG2a/k
Breast carcinoma
Description
E-Cadherin (36) Mouse Monoclonal Primary Antibody is directed against human transmembrane protein E-Cadherin expressed as a part
of the cell-cell adhesion complex in epithelial tissues. Reduction or loss of expression is associated with invasive carcinoma and possibly
metastasis in a variety of carcinomas. This antibody may be used to aid in the differentiation of in situ and/or invasive lobular carcinoma
from in situ and/or invasive ductal carcinoma of the breast.
References
1. Berx G, et al., Breast Cancer Res. 3:289-293, 2001.
2. Van Roy F, et al., Cell Mol Life Sci. 65:3756-3788, 2008.
3. Beavon IRG., European Journal of Cancer. 36:1607-1620, 2000.
4. Siitonen SM, et al., Am J Clin Pathol. 105: 394-402, 1996.
5. Geza A, et al., Am J Clin Pathol. 115:85-98, 2001.
E-Cadherin
(EP700Y)
Part Number
Type
Control
Localisation
Quantity
Isotype
6. Choi YJ, et al., Mod Pathol. 21:1224-1237, 2008.
7. Qureshi HS, et al., Am J Clin Path. 125:377-385, 2006.
8. Kowalski PJ, et al., Breast Cancer Res. 5:R217-R222, 2003.
9. Abutaily AS, et al., J Pathol. 201:355-362, 2003.
760-4440 05973872001
Rabbit Monoclonal
Pancreas, Lung Adenocarcinoma, Breast
Membranous
50 tests
IgG
Infiltrating carcinoma of the breast
Description
E-Cadherin is an adhesion protein that is expressed in cells of epithelial lineage. E-Cadherin (EP700Y) Rabbit Monoclonal Primary Antibody
stains positively in glandular epithelium as well as adenocarcinomas of the lung, GI tract and ovary. It has been useful in distinguishing
adenocarcinoma from mesothelioma. Another application involves the differentiation of ductal (which stains positive) vs. lobular cancer of
the breast. It has also been shown to be positive in some thyroid carcinomas. Loss of E-Cadherin expression has been suggested to be a
poor prognostic sign in breast carcinoma and non-small cell lung carcinoma (NSCLC).
References
1. Krishnadath KK, et al., J Pathol. 182(3):331-8, 1997.
2. Schofield K, et al., Cancer. 81(5):293-8, 1997.
3. Han AC, et al., Hum Pathol. 28(6):641-5, 1997.
4. Simsir A, et al., Diagn Cytopathol. 20(3):125-30, 1999.
5. Han AC, et al., Cancer. 87(2):83-6, 1999.
6. Lear MP, et al., Histopathology. 32(3):209-16, 1998.
7. Karayiannakis AG, et al., Hepatogastroenterology. 45(24):
2437-42, 1998.
8. Peralta Soler A, et al., Hum Pathol. 28(6):734-9, 1997.
9. Abutaily AS, et al., J Clin Pathol. 55(9):662-8, 2002.
10. Wahed A, et al., Ann Diagn Pathol. 6(6):349-51, 2002.
11. Acs G, et al., Am J Clin Pathol. 115(1):85-98, 2001.
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EGFR (Epidermal Growth Factor Receptor), CONFIRM
(3C6)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-2988 05278341001
Mouse Monoclonal
Colorectal Adenocarcinoma, Skin
Membranous
50 tests
IgG1
Squamous cell carcinoma
Description
CONFIRM Epidermal Growth Factor Receptor (EGFR) (3C6) Mouse Monoclonal Primary Antibody is directed against the extracellular
domain of human EGFR, a protein of 170 kD. The antibody also recognises the type III variant of 145 kD. EGFR is a member of the receptor
tyrosine kinase HER family. Also known as HER1, EGFR is structurally related to HER2, HER3 and HER4. Members of this family are
capable of forming heterodimers to elicit signal transduction. EGFR is expressed at low levels in most normal epithelial tissues, but can
be overexpressed in cancers of the breast, brain, bladder, lung, gastric, head and neck, oesophagus, cervix, ovary, endometrium and
particularly in squamous cell carcinomas.
References
1. Ciardello F, et al., Eur J Cancer. 39:1348-54, 2003.
EGFR (Epidermal Growth Factor Receptor), CONFIRM
(5B7)
Part Number
Type
Control
Localisation
Quantity
790-4347 05278457001
Rabbit Monoclonal
Skin, Non-Small Cell Lung Carcinoma, Placenta
50 tests
Squamous cell carcinoma, lung
Description
CONFIRM EGFR (5B7) Rabbit Monoclonal Primary Antibody is produced against a synthetic peptide from the carboxy terminal
(cytoplasmic) region of EGFR. The epitope recognised by EGFR (5B7) is associated with an internal domain of EGFR and therefore EGFR
staining with antibodies that recognise extracellular domains may not always correlate with EGFR (5B7) staining. EGFR is a member of the
receptor tyrosine kinase HER family. Also known as HER 1, EGFR is structurally related to HER 2, HER 3 and HER 4. Members of this protein
family are capable of forming heterodimers to mediate signal transduction. EGFR is expressed at low levels in most normal epithelial
tissues, but can be overexpressed in cancers of the breast, brain, bladder, lung, gastric, head and neck, oesophagus, cervix, ovary,
endometrium and particularly in squamous cell carcinomas.
References
1. Ciardello F, et al., Eur J Cancer. 39:1348-54, 2003.
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EMA (Epithelial Membrane Antigen), CONFIRM
(E29)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4463 05878900001
Mouse Monoclonal
Normal Pancreas
50 tests
IgG2a
Breast, ductal carcinoma
Description
CONFIRM EMA (E29) Mouse Monoclonal Primary Antibody is directed against epithelial membrane antigen expressed on the apical
surface of glandular epithelia. This glycoprotein is primarily membrane localised on normal and tumour tissue, although it may exhibit a
cytoplasmic localisation in less differentiated carcinomas. This antibody may be used to aid in the identification of epithelial membrane
antigen in adenocarcinomas derived from secretory epithelia, malignant mesothelioma, renal cell carcinomas and meningiomas.
References
1. Heyderman E, et al., Br J Cancer. 52:355-361, 1985.
2. Cordell J, et al., Br J Cancer. 52:347-354, 1985.
3. Metze D, et al., J Cutan Pathol. 23:518-529, 1996.
4. Dejmek A, et al., Cytopathol. 11:8-17, 2000.
5. Murakata LA, et al., Mod Pathol. 13:874-881, 2000.
6. Schnitt SJ, et al., Amer J Surg Pathol. 10:640-649, 1986.
7. Gupta RK, et al., Cytopathol. 11:312-321, 2000.
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Ep-CAM/Epithelial Specific Antigen
(Ber-EP4)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4383 05435676001
Mouse Monoclonal
Columnar Epithelium, Adenocarcinoma
Cytoplasmic
50 tests
IgG1/K
Colon carcinoma
Description
Ep-CAM consists of two glycoproteins, 34 and 39 kDa, sometimes designated epithelial antigen, epithelial specific antigen and epithelial
glycoprotein. The glycoproteins are located on the cell membrane surface and in the cytoplasm of virtually all epithelial cells with the
exception of most squamous epithelia, hepatocytes, renal proximal tubular cells, gastric parietal cells and myoepithelial cells. However,
focal positivity may be seen in the basal layer of squamous cell epithelium of endoderm (e.g. palatine tonsils) and mesoderm (e.g. uterine
cervix). In liver lesions like hepatitis and cirrhosis, the hepatocytes frequently become Ep-CAM positive. Normal mesothelial cells are EpCAM negative, but may express focal reaction when undergoing reactive changes. Mesenchymal cells and cells from the neural crest are
negative, with the exception of olfactory neurons.
Ep-CAM is found in the large majority of adenocarcinomas of most sites (50-100% in various studies) as well as neuroendocrine tumours,
including small cell carcinoma. Renal cell carcinoma and hepatocellular carcinoma stain in about 30% of the cases. Squamous cell
carcinoma of endoderm and mesoderm is usually Ber-EP4 positive, while that of ectoderm is negative. Basal cell and basosquamous
carcinoma are Ber-EP4 positive in almost all cases. Choroid plexus papilloma and carcinoma are usually negative. Malignant mesothelioma
(epithelioid and biphasic) is Ep-CAM positive in 4-26% of the cases. The staining is usually focal, but may occasionally be widespread.
Synovial sarcoma (epitheloid and biphasic) and desmoplastic small round cell tumour stain for Ep-CAM in most cases.
Seminoma, embryonal carcinoma, yolk sac tumour and choriocarcinoma reveal Ber-EP4 positivity in a minor proportion of cases. Among
neural tumours, Ep-CAM positivity is seen only in olfactory neuroblastoma. Ep-CAM can be of great help in the differential diagnosis of
malignant involvement in the peritoneal and pleural cavities. The lack of reactivity in the majority of malignant mesothelioma can in an
appropriate panel be utilised in the discrimination between this tumour and adenocarcinoma.
References
1. Latza, et al., J Clin Pathol. 43:213-19, 1990.
2. Ma CK, et al., Am J Clin Pathol. 99(5):551-7, 1993.
3. Carella R, et al., Am J Surg Pathol. 25(1):43-50, 2001.
4. Ordóñez NG, Adv Anat Pathol. 13(1):16-25, 2006.
5. Ordóñez NG, Mod Pathol. 19(3):417-28, 2006.
6. Ordonez NG, Am J Clin Pathol. 109(1):85-89, 1998.
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Epithelial Related Antigen NEW
(MOC-31)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4561 06374409001
Mouse Monoclonal
Kidney, Breast, Lung and Colon
50 tests
IgG1
Lung adenocarcinoma
Description
Epithelial Related Antigen (MOC-31) Mouse Monoclonal Primary Antibody is directed against the 40kD epithelial cell adhesion molecule,
Epithelial Related Antigen, expressed in most normal epithelial cells in tissues including lung, breast, pancreas and colon. This antibody
exhibits a membranous and/or cytoplasmic staining pattern in neoplastic and normal tissue. Epithelial Related Antigen (MOC-31) antibody
may be used as an aid in the differentiation of lung adenocarcinoma and mesothelioma.
References
1. Oates J, et al. Histopathology. 36:341-347, 2000.
2. Morrison C, et al. Mod Pathol. 15:1279-1287, 2002.
3. Hecht JL, et al. Cancer Cytopathology. 108:56-59, 2005.
4. De Leij L, et al. Int J Cancer Suppl. 8:60-63, 1994.
5. Stauss H, et al. CRC Press; 2003.
6. Ordońez N. Am J Surg Pathol. 27:1031-1051, 2003.
ERG NEW
(EPR3864)
Part Number
Type
Control
Localisation
Quantity
7. Pu RT, et al. Diagnostic Citopathology. 36:20-25, 2007.
8. Porcell AI, et al. Mod Pathol. 13:773-778, 2000.
9. King JE, et al. Histopathology. 48:223-232, 2006.
10. Winter MJ, et al. Am J Pathol. 163:2139-2148, 2003.
11. Bartnetson RJ, et al. Am J Clin Pathol. 125:67-76, 2006.
790-4576 06478450001
Rabbit Monoclonal
Spleen
Nuclear, Cytoplasmic
50 tests
Prostate carcinoma
Description
ERG (EPR3864) Rabbit Monoclonal Primary Antibody is directed against the C-terminus of the ETS transcription regulator, ERG and is
capable of detecting both wildtype ERG and truncated ERG resulting from ERG gene rearrangement. This antibody exhibits a nuclear
staining pattern and may be used to aid in the identification of prostate adenocarcinomas through the detection of truncated ERG.
References
2. Perner S, et al. Am J Surg Pathol, 31:882-888. 2007.
3. Carver B, et al. Nature Genet. 41(5):619-624. 2009.
4. Mosquera J-M, et al. J Pathol. 212:91-101. 2007.
5. Park K, et al. Neoplasia12, (7):590-598. 2010.
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Estrogen Receptor (ER), CONFIRM
(SP1)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4324 05278406001 (50 tests);
790-4325 05278414001 (250 tests)
Rabbit Monoclonal
Breast
Nuclear
50 Tests (790-4324), 250 Tests (790-4325)
IgG
Breast lobular carcinoma
Description
CONFIRM Estrogen Receptor (ER) (SP1) Rabbit Monoclonal Primary Antibody (IgG) is intended for the qualitative detection of estrogen
receptor (ER) antigen. ER (SP1) antibody is directed against an epitope present on human ER protein located in the nucleus of ER positive
normal and neoplastic cells. This antibody is indicated as an aid in the management, prognosis and prediction of therapy outcome of breast
cancer.
References
1. Huang Z, et al., Appl Immun Mol Morph. 13(1): 91-5, 2005.
2. PDQ. http://icisun.nci.nih…t_cancer_Physician.html.
3. Stierer M, et al., Cancer Res and Treat. 36:11-21, 1995.
4. Cheang M, et al., J Clin Oncol. 24:5637-5644, 2006.
Factor VIII Related Antigen
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2642 05267528001
Rabbit Polyclonal
Skin, Placenta
Cytoplasmic
50 tests
Placenta
Description
Factor VIII-Related Antigen Rabbit Polyclonal Primary Antibody reacts with endothelial cells and neoplastic blood cells. This antibody
has helped to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi’s sarcoma and cardiac myxoma. Not
all endothelial cells synthesise (or store) this molecule; therefore, it should be expected that not all tumours of endothelial differentiation
(benign or malignant) react with this antigen.
References
1. Wick MR, et al., Lab Invest. 52:75A, 1985.
2. Bhawan J, et al., Cancer. 55:570-576, 1985.
3. Ansell J, et al., Cancer. 50:1506-1512, 1982.
4. Fulling KH, et al., Cancer. 51:1107-1118, 1983.
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Factor XIIIa
(AC-1A1)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4384 05435692001
Mouse Monoclonal
Dermatofibroma
Cytoplasmic
50 tests
IgG1
Dermatofibroma
Description
Factor XIIIa is a blood proenzyme that has been identified in platelets, megakaryocyte and fibroblastlike mesenchymal or histiocytic
cells present in the placenta, uterus and prostate; it is also present in monocytes and macrophages and dermal dendritic cells. Factor
XIIIa antibody has been found to be useful in differentiating between dermatofibroma (90% +), dermatofibrosarcoma protuberans
(25% +) and desmoplastic malignant melanoma (0% +). Factor XIIIa positivity is also seen in capillary hemagioblastoma (100% +),
hemangioendothelioma (100% +), hemangiopericytoma (100% +), xanthogranuloma (100% +), xanthoma (100% +), hepatocellular
carcinoma (93% +), glomus tumour (80% +) and meningioma (80% +).
References
1. Abenoza P, et al., Am J Dermatopathol. 15(5):429-34, 1993.
2. Anstey A, et al., Am J Dermatopathol. 16(1):14-22, 1994.
3. Glusac EJ, et al., Am J Surg Pathol. 18(6): 583-90, 1994.
4. Nemes Z, Hum Pathol. 23(7):805-10, 1992.
5. Demetris AJ, et al., Am J Surg Pathol. 21(3):263-70, 1997.
Factor XIIIa
(EP3372)
Part Number
Type
Control
Localisation
Quantity
Isotype
6. Horenstein MG, et al., Am J Surg Pathol. 24(7):996-1003, 2000.
7. Kraus MD, et al., Am J Dermatopathol. 23(2):104-11, 2001.
8. Dehner LP, Am J Surg Pathol. 27(5):579-93, 2003.
9. Deguchi M, et al., Arch Dermatol Res. 294(7):297-302, 2002.
760-4441 05922470001
Rabbit Monoclonal
Dermatofibroma
Cytoplasmic
50 tests
IgG
Placenta, normal
Description
Factor XIIIa is a blood proenzyme that has been identified in platelets, megakaryocytes and fibroblast-like mesenchymal or histiocytic
cells present in the placenta, uterus and prostate; it is also present in monocytes and macrophages and dermal dendritic cells. Factor
XIIIa antibody has been found to be useful in differentiating between dermatofibroma (90% +), dermatofibrosarcoma protuberans
(25% +) and desmoplastic malignant melanoma (0% +). Factor XIIIa positivity is also seen in capillary hemagioblastoma (100% +),
hemangioendothelioma (100% +), hemangiopericytoma (100% +), xanthogranuloma (100% +), xanthoma (100% +), hepatocellular
carcinoma (93% +), glomus tumour (80% +) and meningioma (80% +).
References
1. Abenoza P, et al., Am J Dermatopathol. 15(5):429-34, 1993.
2. Anstey A, et al., Am J Dermatopathol. 16(1):14-22, 1994.
3. Glusac EJ, et al., Am J Surg Pathol. 18(6):583-90, 1994
4. Nemes Z, et al., Hum Pathol. 23(7):805-10, 1994.
5. Demetris AJ, et al., Am J Surg Pathol. 21(3):263-70, 1997.
6. Horenstein MG, et al., Am J Surg Pathol. 24(7):996-1003, 2000.
7. Kraus MD, et al., Am J Dermatopathol. 23(2):104-11, 2001.
8. Dehner LP, et al., Am J Surg Pathol. 27(5):579-93, 2003.
9. Deguchi M, et al., Arch Dermatol Res. 294(7):297-302, 2002.
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Fascin
(55k-2)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-2702 05268117001
Mouse Monoclonal
Hodgkin’s Lymphoma, Lymph Node
Cytoplasmic
50 tests
IgG1
Description
Fascin (55k-2) Mouse Monoclonal Primary Antibody is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis,
mixed cellularity and lymphocyte depletion Hodgkin’s disease. It is uniformly negative in lymphoid cells, plasma cells and myeloid cells.
Fascin antibody is positive in dendritic cells. This marker may be helpful in distinguishing between Hodgkin’s disease and non-Hodgkin’s
lymphoma in difficult cases. Also, the lack of expression of fascin in the neoplastic follicles in follicular lymphoma can be helpful in
distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased.
References
1. Pinkus GS, et al., Am J of Pathol. 150(2):543-562, 1997.
FSH (Follicle Stimulating Hormone)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2710 05268192001
Rabbit Polyclonal
Normal Pituitary
Cytoplasmic
50 tests
Pituitary
Description
Follicle Stimulating Hormone (FSH) Rabbit Polyclonal Primary Antibody is a useful marker in classification of pituitary tumours and the
study of pituitary disease. It reacts with FSH-producing cells (gonadotrophs).
References
1. Schmid M, et al., Pathol Res Pract. 197(10):663-9, 2001.
2. Uccella S, et al., Pituitary. 3(3):131-9, 2000.
3. La Rosa S, et al., Virchows Arch. 437(3):264-9, 2000.
4. Zheng W, et al., Gynecol Oncol. 76(1):80-8, 2000.
5. Ben-Josef E, et al., J Urol. 161(3):970-6, 1999.
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Galectin-3
(9C4)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4256 05269164001
Mouse Monoclonal
Papillary or Follicular Carcinoma of Thyroid
Cytoplasmic
50 tests
IgG1
Papillary carcinoma of the thyroid
Description
Galectin-3 is a 31 kD beta-galactosidase binding lectin. It has been associated with binding to the basement membrane glycoprotein
laminin. Galectin-3 (9C4) Mouse Monoclonal Primary Antibody has been demonstrated to be valuable in differentiating between benign
and malignant thyroid neoplasms in both histologic sections and fine needle aspiration biopsy material. Galectin-3 antibody has also been
useful in identifying anaplastic large cell lymphoma.
References
1. Inohara H, et al., Cancer. 85:2475-84, 1999.
2. Herrmann ME, et al., Arch Pathol Lab Med. 126:710-713, 2002.
3. Papotti M, et al., Eur J of Endo. 147:515-521, 2002.
Gastrin
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
4. Bartolazzi A, et al., Lancet. 357:1644-50, 2001.
5. Orlandi F, et al., Cancer Res. 58:3015-3020, 1998.
6. Gasbarri A, et al., J Clin Oncol. 17:3494-3502, 1999.
760-2643 05267536001
Rabbit Polyclonal
Stomach
Cytoplasmic
50 tests
Stomach
Description
Gastrin Rabbit Polyclonal Primary Antibody gives positive staining of G cells of human antral/pyloric mucosa and cells producing gastrin
or a structural gastrin analogue as is seen in the stomach; no staining of other cells or tissue types has been observed. This antibody may
react with sulphated and non-sulphated forms of gastrin. The antibody cross-reacts with more than 50% of the present choleocystokinin
octa peptide.
References
1. Rehfeld JF, et al., J Biol Chem. 256:10426-9, 1981.
2. Kirchner T, et al., Am J Surg Path. 11:909-17, 1987.
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GCDFP-15
(EP1582Y)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4386 05463530001
Rabbit Monoclonal
Breast, Breast Carcinoma
Cytoplasmic
50 tests
IgG
Breast carcinoma
Description
GCDFP-15 is a 15,000-dalton glycoprotein which is localised in the apocrine metaplastic epithelium lining breast cysts and in apocrine
glands in the axilla, vulva, eyelid and ear canal. Approximately 70% of breast carcinomas stain positive with antibody to GCDFP-15.
Colorectal carcinomas, as well as mesotheliomas, do not stain with this antibody. Lung adenocarcinoma rarely stains with this antibody.
References
1. Mazoujian G, et al., Am J Dermatopathol. 10(1):28-35, 1988.
2. Ansai S, et al., Am J Dermatopathol. 17(3):249-55, 1995.
3. Mazoujian G, et al., Am J Pathol. 110(2):105-12, 1983.
4. Wich MR, et al., Hum Pathol. 20(3):281-7, 1989.
5. Cohen C, et al., Arch Pathol Lab Med. 117(3):291-4, 1993.
6. Raju U, et al., Mod Pathol. 6(5):516-20, 1993.
7. Bhargava R, et al., Am J Clin Pathol. 127:103-113, 2007.
8. Tornos C, et al., Am J Surg Pathol. 29(11):1482-9, 2005.
9. Takeda Y, et al., Arch Pathol Lab Med. 132(2):239-43, 2008.
10. Liegl B, et al., Histopathology. 50(4):439-47, 2007.
GFAP (Glial Fibrillary Acidic Protein)
(EP672Y)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4345 05269784001
Rabbit Monoclonal
Brain
Cytoplasmic
50 tests
IgG
Astrocytoma
Description
GFAP (EP672Y) Rabbit Monoclonal Primary Antibody detects astrocytes, Schwann cells, satellite cells, enteric glial cells and some groups
of ependymal cells. This marker is mainly used to distinguish neoplasms of astrocytic origin from other neoplasms in the central nervous
system.
References
1. Viale G, et al., Virchow’s Arch A Pathol Anat. 418:339-348, 1991.
2. Choi BH, et al., Science. 223:407-409, 1984.
3. Funata N, et al., Bull Tokyo Med Dent Univ. 32:9-18, 1985.
4. Jessen KR, et al., J Neurosci. 3:2206-2218, 1983.
5. Kawahara E, et al., Am J Surg Pathol. 12:115-120, 1988.
6. Nagashima G, et al. Clin Neurol Neurosurg. 2002
May;104(2):125-31.
7. Regner A, et al. J Neurotrauma. 2001 Aug;18(8):783-92.
8. Giangaspero F, et al. Acta Neuropatahol (Berl). 2002
Feb;103(2):152-6. Epub 2001 Nov 10.
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GH (Growth Hormone)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2804 05268257001
Rabbit Polyclonal
Normal Pituitary
Cytoplasmic
50 tests
Pituitary
Description
Growth Hormone Rabbit Polyclonal Primary Antibody is a useful marker in classification of pituitary tumours and the study of pituitary
disease (acromegaly). It reacts with GH-producing cells.
References
1. Fukaya T, et al., Cancer. 45:1598, 1980.
2. Kovacs K, et al., Virch Arch Pathol Anat. 395:59, 1982.
3. Cunha KS, et al., J Clin Pathol. 56(10):758-63, 2003.
Glucagon
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
4. Chopin LK, et al., Growth Horm. IGF. Res. 12(2):126-36, 2002.
5. Matsuno A, et al., Pathol Res Pract. 197(1):13-20, 2001.
6. Garcia-Caballero T, et al., Endocrine. 12(3):265-71, 2000.
760-2644 05267544001
Rabbit Polyclonal
Pancreas
Cytoplasmic
50 tests
Pancreas, islets of Langerhans
Description
Glucagon Rabbit Polyclonal Primary Antibody detects glucagon-secreting cells and tumours such as glucagonomas. Studies show that
approximately 80% of glucagonomas are malignant and these patients have a syndrome often initially recognised by dermatologists.
Symptoms include necrolytic migratory erythema as well as diabetes, anemia, stomatitis, weight loss, frequent venous thromboses and in
some instances diarrhoea and psychiatric disturbances. The diagnosis may be readily confirmed by the demonstration of elevated plasma
glucagon concentration.
References
1. Unger RH, et al., N Eng J Med. 304:1518-1524, 1981.
2. Larson L, et al., Hum Pathol. 9:401-416, 1978.
3. Erlandsen SL, Williams and Wilkins, Baltimore, 40-155, 1980.
4. Friesen SR, et al., N Eng J Med. 306:580-590, 1982.
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GLUT 1 NEW
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
760-4526 06419178001
Rabbit Polyclonal
Malignant Mesothelioma, Colorectal Carcinoma
Membranous
50 tests
Description
Glucose transporter type I (GLUT1), a prototype member of GLUT super family, reacts with a 55 kD protein and is a membrane-associated
erythrocyte glucose transport protein. It is a major glucose transporter in the mammalian blood-brain barrier and also mediates glucose
transport in endothelial cells of the vasculature, adipose tissue and cardiac muscle. GLUT1 is detectable in many human tissues including
those of colon, lung, stomach, oesophagus and breast. GLUT1 is overexpressed in malignant cells and in a variety of tumours that include
breast, pancreas, cervix, endometrium, lung, mesothelium, colon, bladder, thyroid, bone, soft tissues and oral cavity.
References
1. Kato Y, et. al., Mod Pathol. 20:215-220, 2007.
2. Afify A, et. al., Acta Cytol. 49:621-626, 2005.
Glycophorin A
(GA-R2 & HIR2)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Parente P, et. al. J Exp Clin Can Res. 27;34, 2008.
4. Zimmerman RL, et. al., Cancer. 96: 53-57, 2002.
760-4257 05269172001
Mouse Monoclonal
Bone Marrow
Membranous
50 tests
IgG2b/K
Bone marrow, erythroid elements
Description
Glycophorin A is a sialoglycoprotein present on human red blood cells and their precursors. Glycophorin A (GA-R2 & HIR2) Mouse
Monoclonal Primary Antibody has been used to characterise erythroid cell development and in the diagnosis of erythroid leukaemia.
References
4. Chang CC, et al., Am J Clin Pathol. 114(5):807-11, 2000.
1. van der Valk P, et al., Am J Surg Pathol. 13(2): 97-106, 1989.
2. Muller M, et al., J Vet Med A Physiol Pathol Clin Med. 48(1):51-7, 2001. 5. Muller M, et al., J Vet Med A Physiol Pathol Clin Med. 48(1):51-7, 2001.
3. Sadahira Y, et al., J Clin Pathol. 52(12):919-21, 1999.
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Glypican-3
(1G12)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4442 05973864001
Mouse Monoclonal
Hepatocellular Carcinoma
Cytoplasmic
50 tests
IgG1
Description
Glypican-3 (GPC3) is a glycosylphosphatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Recently,
GPC3 was identified as a useful tumour marker for the diagnosis of HCC (hepatocellular carcinoma), hepatoblastoma, melanoma, testicular
germ cell tumours and Wilms tumour. In patients with HCC, GPC3 was overexpressed in neoplastic liver tissue and elevated in serum,
but was undetectable in normal liver, benign liver and the serum of healthy donors. GPC3 expression was also found to be higher in HCC
liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant
transformation. In the context of testicular germ cell tumours, GPC3 expression is up-regulated in certain histologic subtypes, specifically
yolk sac tumours and choriocarcinoma. A high level of GPC3 expression has also been found in some types of embryonal tumours, such as
Wilms’ tumour and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue.
References
1. Capurro M, et al., Gastroenterology. 125(1):89-97, 2003.
2. Coston WMP, et al., Am J Surg Pathol. 32(3):433-444, 2008.
3. Kandil D, et al., Cancer. 111(5):316-22, 2007.
Glypican 3 NEW
(GC33)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Kakar S, et al., Arch Pathol Lab Med. 131(11):1648-54, 2007.
Review.
5. Libbrecht L, et al., Am J Surg Pathol. 30(11):1405-11, 2006.
790-4564 06483186001
Mouse Monoclonal
Placenta, Hepatocellular Carcinoma
Granular Cytoplasmic/Membrane
50 tests
IgG2a
Description
Glypican 3 (GC33) Mouse Monoclonal Primary Antibody is directed against the heparin sulphate proteoglycan, glypican 3. This antibody
may be used to aid in the differentiation of hepatocellular carcinoma from normal liver or benign lesions.
References
1. Akutsu, N, et al. World J Gastroenterol. 16,28;3521-3528, 2010.
2. Ishiguro, T, et al. Cancer Res. 68,23;9832-9838, 2008.
3. Capurro MI, et al. Cancer Res. 65;6245-6254, 2005.
4. Midorikawa Y, et al. Int J Cancer. 103;455-465, 2003.
5. Stigliano I, et al. Breast Cancer Res Treat. 114;251-262, 2009.
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Granzyme B
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
<PREV
NEXT>
760-4283 05269466001
Rabbit Polyclonal
Tonsil
Granular Cytoplasmic, Cytotoxic T-Lymphocytes, Natural Killer Cells
50 tests
Tonsil
Description
Granzymes are serine proteases which are stored in specialised lytic granules of cytotoxic T-lymphocytes and in natural killer cells.
Granzyme B Rabbit Polyclonal Primary Antibody has been useful in diagnosing natural killer/T-cell lymphoma as well as anaplastic large
cell lymphoma. High percentages of cytotoxic T cells have been shown to be an unfavourable prognostic indicator in Hodgkin’s disease.
References
1. Oudejans JJ, et al., Blood. 89(4):1376-82, 197.
2. Oudejans JJ, et al., Am J Pathol. 148(1):233-40, 1996.
3. Liu J, et al., J Dermatol. 30(10):735-41, 2003.
4. Kato N, et al., Am J Dermatopathol. 25(2):142-7, 2003.
5. Kummer JA, et al., Clin Exp Immunol. 100:164-172, 1995.
Helicobacter pylori, CONFIRM
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
790-4374 05926092001 (50 tests);
790-1009 05926084001 (250 tests)
Rabbit Monoclonal
Infected Stomach Mucosa
Cell Wall of H. pylori
50 tests and 250 tests
Gastric mucosa, H. pylori
Description
CONFIRM Helicobacter pylori (SP48) Rabbit Monoclonal Primary Antibody is designed to qualitatively detect the presence of Helicobacter
pylori in gastric biopsy tissue. Immunohistochemical staining with this antibody may aid in the diagnosis of Helicobacter pylori infection.
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HER-2/neu, PATHWAY fda approved
(4B5)
Part Number
Type
Control
Localisation
Quantity
790-2991 05278368001
Rabbit Monoclonal
Breast Carcinoma
Membranous
50 tests
Breast carcinoma
Description
This antibody is intended for in vitro diagnostic use. PATHWAY anti-HER-2/neu (4B5) Rabbit Monoclonal Primary Antibody (PATHWAY HER-2 (4B5))
is a rabbit monoclonal antibody intended for laboratory use for the semi-quantitative detection of HER-2 antigen in sections of formalin-fixed,
paraffin-embedded normal and neoplastic tissue on a VENTANA automated immunohistochemistry slide staining device. It is indicated as an aid
in the assessment of breast cancer patients for whom Herceptin treatment is considered. Note: All of the patients in the Herceptin clinical trials
were selected using a clinical trial assay. None of the patients in those trials were selected using PATHWAY anti-HER-2/neu (4B5). PATHWAY antiHER-2/neu (4B5) was compared to PATHWAY HER-2 (clone CB11) Primary Antibody on an independent sample set and found to provide acceptably
concordant results. The actual correlation of PATHWAY anti-HER-2/neu (4B5) to clinical outcome has not been established.
PATHWAY anti-HER-2/neu is a rabbit monoclonal antibody (clone 4B5) directed against the internal domain of the c erbB 2 oncoprotein (HER2). c erbB 2 oncoprotein was cloned and characterised by Akiyama et al in 1986.1 It is an approximately 185 kD transmembrane glycoprotein
which is structurally similar to epidermal growth factor receptor (EGFR). The protein is associated with tyrosine kinase activity similar to that of
several growth factor receptors and to that of the transforming proteins of the src family. The coding sequence is consistent with an extracellular
binding domain and an intracellular kinase domain. This suggests that HER-2 may be involved in signal transduction and stimulation of mitogenic
activity.1 Clone 4B5 has been shown to react with a 185 kD protein from SK BR 3 cell lysates via Western blotting. SK BR 3 is a breast carcinoma
cell line which has a 128 fold overexpression of HER-2 mRNA.2 The size of the band identified correlates well with that reported by Akiyama et al
for HER-2 protein (185 kD).1 Immunohistochemistry has been used to detect specific antigens in cells or tissue since 1950.3 The use of enzymes
and peroxidase as markers for immunohistochemistry was reported by Nakane and Pierce in 1967.4 The increased sensitivity of the avidin biotin
peroxidase detection system over the enzyme labelled antibody method was documented by Hsu et al in 1981.5 The HER-2 protein is expressed
at a level detectable by immunohistochemistry in up to 20 percent of adenocarcinomas from various sites. Between 15 and 30 percent of invasive
ductal cancers are positive for HER-2.6 Almost all cases of Paget’s disease of breast7 and up to 90 percent of cases of ductal carcinoma in situ of
comedo type are positive.6 The immunohistochemical detection of HER-2 protein overexpression is also used as an aid in determination of patients
for whom Herceptin therapy is indicated.8 Staining results in normal tissues, neoplastic tissues and 322 cases of breast carcinoma with PATHWAY
HER-2 (4B5) were evaluated by Ventana Medical Systems, Inc. In the normal tissues tested, expression was consistent with the published litreature
in that there was no unexpected specific cytoplasmic/membrane staining, with the following exceptions: two cases of tonsil showing with epithelial
cell membrane staining, one case of parathyroid and one case of oesophageal epithelium. Of the neoplastic tissues tested, cytoplasmic/membrane
staining was seen in cancer cells of the breast, colon and ovary. Three hundred twenty-two (322) breast carcinomas were evaluated with
PATHWAY HER-2 (4B5) in a method comparison study with PATHWAY HER-2 (CB11). There is a significant correlation of staining between these
two tests. See Summary of Expected Results section for further information. PATHWAY HER-2 (4B5) in combination with iVIEW DAB Detection Kit,
utilises biotinylated secondary antibodies to locate the bound PATHWAY HER-2 (4B5) primary antibody (produced by using a synthetic peptide
corresponding to a site on the internal domain of the HER-2 protein). This is followed by the binding of an avidin/streptavidin enzyme conjugate to
the biotin. The complex is then visualised using a precipitating enzyme generated product. The use of pre-diluted PATHWAY HER-2 (4B5) and ready
to use iVIEW DAB detection kits, in combination with a VENTANA automated slide stainer, reduces the possibility of human error and inherent
variability resulting from individual reagent dilution, manual pipetting and manual reagent application.
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Clinical Significance
Breast cancer is the most common carcinoma occurring in women and the second leading cause of cancer-related death. In North America,
a woman’s chance of contracting breast cancer is one in eight.9 Early detection and appropriate treatment therapies can significantly affect
overall survival.10 Small tissue samples may be easily used in routine immunohistochemistry (IHC), making this technique, in combination with
antibodies that detect antigens important for carcinoma interpretation, an effective tool for the pathologist in his/her diagnosis and prognosis
of disease. One important marker in breast cancer today is cerbB 2 oncoprotein (HER-2). HER-2 is an intracellular membrane protein detected
in the cellular membrane.11 It is closely related to EGFR and, like EGFR, has tyrosine kinase activity.1 Gene amplification and the corresponding
overexpression of cerbB 2 has been found in a variety of tumours, including breast carcinomas.11,12 The therapeutic drug Herceptin has
been shown to benefit some breast carcinoma patients by arresting and in some cases reversing the growth of their cancer.8 The drug is a
humanised monoclonal antibody that binds to HER-2 protein on cancer cells. Thus only patients with HER-2/neu positive breast carcinomas
should benefit from treatment with Herceptin. In vitro diagnostics for the determination of HER-2 status in breast carcinomas are important
to aid the clinician in determination of therapy with Herceptin. Interpretation of the results of any detection system for HER-2 must take into
consideration the fact that HER-2 is expressed in both breast cancer tumours and healthy tissue, albeit at differing levels and with different
patterns of expression.13 Histological tissue preparations have the advantage of intact tissue morphology to aid in the interpretation of the HER2 positivity of the sample. All histological tests should be interpreted by a specialist in breast cancer morphology and/or pathology and the
results should be complemented by morphological studies and proper controls and used in conjunction with other clinical and laboratory data.
References
1.Akiyama T, et al., Science 232: 1644-1646, 1986.
2. Kraus MH, et al., EMBO. 6: 605-610, 1987.
3. Coons AH, et al., J. Exp. Med. 91: 1-13, 1950.
4. Nakane PK, et al., J. Histochem. Cytochem. 14:929-931, 1967.
5. Hsu SM, et al., J. Histochem. Cytochem. 29:577-580, 1981.
6. Dickson RB, Lippman, ME, Genes, Oncogenes and Hormones.
Boston, Kluwer Academic Publishers, 1992.
7. Keatings L, et al., Histopathology. 17:234-247, 1990.
8. Herceptin (Trastuzamab) Package Insert. February 2005.
9. Roche PC, Immunohistochemical stains for breast cancer. Mayo
Clin. Proc. 69:57-58, 1994.
10. Charpin C, et al., Br. J. Cancer. 75: 1667-1673, 1997.
11. Corbett IP, et al., J. Pathol. 161:15-25, 1990.
12. Nicholson RI, et al., Eur. J. Cancer. 29A:1018-1023, 1993.
13. DePotter CR, et al., Histopathology. 15:351-362, 1989.
Criteria for Intensity of Cell Membrane Staining with PATHWAY HER-2/neu
Staining Pattern
Score (report to physician)
HER-2 Staining Assessment
No membrane staining is observed
0
Negative
Faint, partial staining in
> 10% of cancer cells with rare or absent circumferential staining
1+
Negative
Weak circumferential membrane staining in
> 10% of cancer cells but the membrane staining ring is thin
2+
Positive
Intense circumferential membrane staining in
> 10% of cancer cells and the membrane staining ring is thick
3+
Positive
1+ Membrane Staining
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HER2/neu, VENTANA NEW
(4B5)
Part Number
Type
Control
Localisation
Quantity
790-4493 05999570001
Rabbit Monoclonal
Breast Carcinoma
Membranous
50 tests
Description
VENTANA HER2/neu (4B5) Rabbit Monoclonal Primary Antibody (VENTANA HER2 - 4B5) is intended for the semi-quantitative detection of
HER2 antigen in sections of formalin-fixed, paraffin-embedded normal and neoplastic breast and gastric tissue on a VENTANA automated
slide staining device. VENTANA HER2 (4B5) is a rabbit monoclonal antibody directed against the internal domain of the c-erbB-2
oncoprotein (HER2). In breast carcinoma, the HER2 protein is expressed at a level detectable by immunohistochemistry in up to 20% of
adenocarcinomas from various sites. Overexpression of the HER2 protein, amplification of the HER2 gene or both occur in approximately
15 to 25 percent of breast cancers and are associated with aggressive tumour behaviour.1 Almost all cases of Paget’s disease of breast 2
and up to 90 percent of cases of ductal carcinoma in situ of comedo type are positive. 3 In gastric carcinoma the HER2 protein is expressed
at a level detectable by immunohistochemistry in up to 30 percent of intestinal type, 15 percent mixed type and 5 percent diffuse type
gastric cancers.4
References
1. Slamon DJ, et al., Human breast cancer: correlation of relapse
and survival with amplification of the HER2/neu oncogene. Science
1987;235:177-182
2. Keatings L, et al., cerbB-2 oncoprotein expression in mammary
and extramammary Paget’s desease: an immunohistochemical study.
Histopathology. 17: 234-247, 1990
3. DIckson RB, Lippman ME, Genes, Oncogenes and Hormones.
Boston: Kluwer Academic Publishers, 1992
4. Herceptin (Trastuzamab) Package Insert. Accessed October
2010
Herpes Simplex Virus I (HSV I)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-4261 05269237001
Rabbit Polyclonal
Infected Tissue
50 tests
Description
Herpes Simplex virus is quite ubiquitous and is quite variable in its presentation in human disease. Type I usually infects the non-genital
mucosal surfaces. It may affect the skin or internal organs (typically brain, lung, liver, adrenal gland or GI tract) of immunocompromised
individuals. HSV I Rabbit Polyclonal Primary Antibody reacts with Type I Herpes viruses. There may be cross-reactivity with Varicella Zoster
virus at higher concentration. Cross-reactivity with the CMV or Epstein-Barr virus is not seen with this antibody,
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Herpes Simplex Virus II (HSV II)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-4262 05269245001
Rabbit Polyclonal
Infected Tissue
50 tests
Follicular lymphoma
Description
Herpes Simplex virus is quite ubiquitous and is quite variable in its presentation in human disease. Type II typically involves the genitalia.
It may affect the skin or internal organs (typically brain, lung, liver, adrenal gland or GI tract) of immunocompromised individuals. HSV II
Rabbit Polyclonal Primary Antibody reacts with Type II Herpes viruses. There may be cross-reactivity with Varicella Zoster virus at higher
concentration. Cross-reactivity with CMV or EBV is not seen with this antibody,
HGAL NEW
(MRQ-49)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4597 06433375001
Mouse Monoclonal
Tonsil, Lymph Node
Cytoplasmic
50 tests
IgG2a/K
Follicular lymphoma
Description
HGAL (MRQ-49) Mouse Monoclonal Primary Antibody used as an aid in the identification of follicular lymphoma within the context of an
antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
References
1. Natkunam, Y et al. Blood. 105:3979–3986, 2005.
2. Natkunam, Y et al. Blood. 109:298-305, 2007.
3. Younes, SF et al. Am J Surg Pathol. 34:1266-1276, 2010.
4. Higgins, RA et al. Arch Pathol Lab Med. 132:441-446, 2008.
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HHV-8 (Human Herpes Virus Type 8)
(13B10)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4260 05269229001
Mouse Monoclonal
Kaposi’s Sarcoma
Nuclear
50 tests
IgG1
Kaposi’s sarcoma
Description
Human herpes virus type 8 (HHV-8) is the likely etiological agent of Kaposi’s sarcoma (KS). HHV-8 DNA sequences have been found
in Kaposi’s sarcoma lesions, primary effusion lymphoma and multicentric Castleman’s disease via polymerase chain reaction and in
situ hybridisation. Latent nuclear antigen (LNA-1, LNA, LANA-1), also known as ORF73, is a 222- or 234 kD protein that is consistently
expressed in HHV-8 infected cells. HHV-8 (13B10) Mouse Monoclonal Primary Antibody labels the latent nuclear antigen protein via
immunohistochemistry.
References
1. Corbellino M, et al., AIDS Res Hum Retroviruses. 12(8):651-7, 1996.
2. Katano H, et al., Am J Pathol. 155(1):47-52, 1999.
3. Katano H, et al., J Med Virol. 59(3):346-55, 1999.
4. Katano H, et al., Mod Pathol. 13(1):77-85, 2000.
5. Kaaya E, et al., Med Oncol. 17(4):325-32, 2000.
HSA (Hepatocyte Specific Antigen)
(0CH1E5)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4350 05269792001
Mouse Monoclonal
Liver
Cytomplasmic
50 tests
IgG1/K
Liver
Description
Hepatocyte Specific Antigen (OCH1E5) Mouse Monoclonal Primary Antibody recognises both benign and malignant liver including such
tumours as hepatoblastoma, hepatocellular carcinoma and hepatic adenoma. It recognises both normal adult and foetal liver tissue.
The typical pattern is a granular cytoplasmic staining. This antibody is useful in differentiating hepatocellular carcinomas with adenoid
features from adenocarcinomas, either primary in the liver or metastatic lesions to the liver. In recognising hepatoblastoma, it is useful in
differentiating this entity from other small round cell tumours.
References
1. Minervini MI, et al., Mod Pathol. 10(7):686-692, 1997.
2. Fasano M, et al., Mod Pathol. 11(10):934-938, 1998.
3. Tsui WMS, et al., Am J Surg Pathol. 23(1): 34-48, 1999..
4. Wieczorek T, et al., Am J Clin Pathol. 118(6):911-21, 2002.
5. Chu PG, et al., Am J Surg Pathol. 26(8):978-88, 2002.
6. Maitra A, et al., Am J Clin Pathol. 115(5):689-94, 2001.
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Human Chorionic Gonadotropin (hCG)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2650 05267617001
Rabbit Polyclonal
Placenta
Cytoplasmic
50 tests
Placenta, cytotrophoblasts
Description
Human chorionic gonadotropin (hCG) is a protein secreted in large quantities by normal trophoblasts. Human Chorionic Gonadotropin
Rabbit Polyclonal Primary Antibody detects cells and tumours of trophoblastic origin such as choriocarcinoma. Large cell carcinoma and
adenocarcinoma of the lung demonstrate hCG positivity in 90% and 60% of cases respectively. 20% of squamous cell lung carcinomas are
positive for hCG. hCG expression by non-trophoblastic tumours may indicate aggressive behaviour since it has been observed that hCG
may play a role in the host response to a given tumour.
References
1. Morrish DW, et al., J Histochem Cytochem. 35:39-101, 1987.
2. Kurman RJ, et al., Cancer. 38:2404-2419, 1976.
3. Kurman RJ, et al., Int J Gyn Pathol. 3:101-12, 1984
4. Boucher LD, et al. Human Pathol. 26(11):1201-6, 1995.
Human Placental Lactogen (hPL)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4443 05973830001
Rabbit Polyclonal
Placenta
Cytoplasmic
50 tests
IgG
Placenta, normal
Description
Human placental lactogen (hPL), also previously known as human chorionic somatomammotropin, is a 22 kD protein with partial homology
to growth hormone. hPL is first detectable in the maternal serum in the fifth week of gestation and reaches a plateau by the thirtyfourth week. hPL has been demonstrated by immunochemistry in the syncytiotrophoblastic cells of choriocarcinoma. A rare variant of
trophoblastic tumour has been reported in the testis with resemblance to uterine placental site trophoblastic tumour. It consisted purely of
intermediate trophoblasts, which was diffusely positive for hPL and focally for B-hCG.
References
1. Shih IM, et al., Am J Surg Pathol. 28(9):1177-83, 2004.
2. Ulbright TM, et al., Am J Surg Pathol. 21(3):282-288, 1997.
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IgA (Immunoglobulin A)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2652 05267625001
Rabbit Polyclonal
Tonsil, Lymph Node
Cytoplasmic
50 tests
Description
IgA Rabbit Polyclonal Primay Antibody reacts with surface immunoglobulin IgA alpha chains. It is useful when identifying leukaemias,
plasmacytomas and B-cell lineage derived Hodgkin’s lymphomas. Due to the restricted expression of heavy and light chains in these
diseases, demonstration of B-cell lymphomas is possible with clonal gene rearrangement studies.
References
1. Arnold A, et al., New Eng J Med. 309:1593-1599, 1983.
2. Geenwich Medical Media Ltd. 217-219, 1999.
3. Hertel BF, et al., New Eng J Med. 302:1293-1297, 1980.
4. Taylor CR, et al., Arch Path Lab Med. 102:113-121, 1978.
5. Warnake R, et al., Masson Publishing USA. 203-221, 1981.
IgD (Immunoglobulin D)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4444 05973821001
Rabbit Polyclonal
Lymph Node, Tonsil
Cytoplasmic
50 tests
IgG
Tonsillitis, sub-epithelial plasma cells
Description
IgD Rabbit Polyclonal Primay Antibody reacts with surface immunoglobulin IgD delta chains. This antibody is useful when identifying
leukaemias, plasmacytomas and B-cell lineage derived lymphomas (in particular marginal zone lymphoma). Cytoplasmic staining is easily
identified on paraffin tissue. Surface immunoglobulin is difficult to demonstrate on paraffin sections but can be demonstrated on frozen
sections.
References
1. Campo E, et al., Am J Surg Pathol. 23(1):59-68, 1999.
2. Mori S, et al., Acta Pathol Jpn. 36(1):1429-40, 1986.
3. Oka K, et al., Acta Haematol. 90(2):84-9, 1993.
4. Bertero M, et al., J Am Acad Dermatol. 30(1):23-30, 1994.
5. Mollego M, et al., Am J Surg Pathol. 21(7):772-80, 1997.
6. Tang X, et al., Pathol Int. 45(1):34-44, 1995.
7. Gupta D, et al., Mod Pathol. 13(11):1161-6, 2000.
8. Anagnostopoulos I, et al., Histopathology. 39(6):561-5, 2001.
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IgG (Immunoglobulin G)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2653 05267633001
Rabbit Polyclonal
Tonsil, Lymph Node
Cytoplasmic
50 tests
Description
IgG Rabbit Polyclonal Primay Antibody reacts with surface immunoglobulin IgG gamma chains. This antibody is useful when identifying
leukaemias, plasmacytomas and B-cell lineage derived Hodgkin’s lymphomas. Due to the restricted expression of heavy and light chains in
these diseases, demonstration of B-cell lymphomas is possible with clonal gene rearrangement studies.
References
5. Warnake R, et al., Masson Publishing USA. 203-221, 1981.
IgM (Immunoglobulin M)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2654 05267641001
Rabbit Polyclonal
Tonsil, Lymph Node
Cytoplasmic
50 tests
Description
IgM Rabbit Polyclonal Primay Antibody reacts with surface immunoglobulin IgM mu chains. IgM is one of the predominant surface
immunoglobulins on B lymphocytes. This antibody is useful when identifying lymphomas, plasmacytomas and B-cell lineage derived
Hodgkin’s lymphomas. Due to the restricted expression of heavy and light chains in these diseases, demonstration of B-cell lymphomas is
possible with clonal gene rearrangement studies.
References
5. Warnake R, et al., Masson Publishing, USA. 203-221, 1981.
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Inhibin, alpha
(R1)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-2834 05268311001
Mouse Monoclonal
Adrenal Cortex, Placenta, Testis
Cytoplasmic
50 tests
IgG2a
Placenta
Description
Inhibin alpha (R1) Mouse Monoclonal Primary Antibody is an antibody against a peptide hormone which has demonstrated utility in the
differentiation between adrenal cortical tumours and renal cell carcinoma. Sex cord stromal tumours of the ovary as well as trophoblastc
tumours also demonstrate cytoplasmic positivity with this antibody.
References
1. Arora DS, et al., J Pathol. 181(4):413-8, 1997.
2. Stewart CJ, et al., Histopathology. 31(1):67-74, 1997.
3. Yamashita K, et al., Am J Obstet Gynecol. 177(6):1450-7, 1997.
Insulin
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
4. McCluggage WG, et al., Am J Surg Pathol. 22(5):615-9, 1998.
5. Kommoss F, et al., Mod Pathol. 11(7):656-64, 1998.
6. Matias-Guiu X, et al., Hum Pathol. 29(8):840-5, 1998.
760-2655 05267650001
Rabbit Polyclonal
Pancreas
Cytoplasmic
50 tests
Description
Insulin is produced in the beta cells of the pancreas. The presence of insulin in the cytoplasm of islet tumours is the most reliable indication
of functional insulinomas. Defective insulin storage occurs in insulinomas, therefore, many sections of the tumour should be stained with
both insulin and C peptide.
References
1. Akagi T, et al., Cancer. 47:417-424, 1981.
2. Scully RE, et al., N Eng J Med. 308:30-37, 1983.
3. Erlandsen SL, et al., Williams and Wilkins, Baltimore. 140-155,
1980.
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Insulin Growth Factor-1 Receptor (IGF-1R), CONFIRM
(G11)
Part Number
Type
Control
Localisation
Quantity
790-4346 05278449001
Rabbit Monoclonal
Breast Carcinoma, Placenta
Membranous
50 tests
Breast carcinoma
Description
CONFIRM IGF-1R (G11) Rabbit Monoclonal Primary Antibody is produced against a synthetic peptide from the internal domain of the beta
chain receptor near the carboxy terminal region and binds to the IGF-1R epitope. IGF-1R is a type 2 tyrosine kinase receptor that shares a
60% homology at the amino acid sequence level with Insulin Receptor (IR).
References
1. Samani A, et al., Endocrine Reviews, 28(1):20-47, 2007.
Kappa, CONFIRM
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2514 05267013001
Rabbit Polyclonal
Tonsil, Lymph Node
Surface Membrane and/or Cytoplasmic
50 tests
Description
CONFIRM Kappa Rabbit Polyclonal Primary Antibody specifically binds to antigens located in the cell membrane and cytoplasmic
regions of normal B cells and plasma cells. Kappa light chains are polypeptide chains which in combination with heavy chains form
immunoglobulin molecules. There are two classes of light chains found in immunoglobulins, kappa light chains and lambda light chains.
Light chain production by lymphoid cells is genetically restricted such that the immunoglobulin molecules produced by an individual cell
will only contain a single light chain class. This clonal restriction may be used to indicate the polyclonal or monoclonal nature of B cell and
plasma cell populations. Because clonality is a clinically important attribute to aid in the subclassification of lymphomas, the identification
of light chain class has gained increasing importance in immunopathology.
References
1. Landaas TO, et al., Acta Path Microbiol Scand. 89(2):91-101, 1981.
2. Pangalis GA, et al., Cancer. 45(6):1334-1339, 1980.
3. Woda BA, et al., Cancer. 43(1):303-307, 1979.
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Ki-67, CONFIRM
(30-9)
Part Number
Type
Control
Localisation
Quantity
<PREV
790-4286 05278384001
Rabbit Monoclonal
Lymph Node, Tonsil
Nuclear
50 tests
Description
CONFIRM Ki-67 (30-9) Rabbit Monoclonal Primary Antibody is directed against C-terminal portion of Ki-67 antigen. Staining for Ki-67 can
aid in assessing the proliferative activity of normal and neoplastic tissue. This antibody is intended for use to identify stained proliferating
cells. Ki-67 is a nuclear protein expressed in proliferating cells. During the cell cycle, the Ki-67 antigen is present in the G1, S, G2 and M
phase, but is absent in the G0 (quiescent) phase.
References
1. Keng PC, et al., Radiat Oncol Investig. 6(3):120-7, 1998.
2. Rey A, et al., Arch Esp Urol. 51(2): 204-10, 1998.
3. Bacchi CE, et al., Braz J Med Biol Res. 26(7):677-87, 1993.
Ksp-Cadherin
(MRQ-33)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Alliegro M, et al., Exp Cell Res. 279(1):111-17, 2002.
5. Scholzen T, et al., J Cell Physiol. 182(3):311-22, 2000.
760-4387 05463548001
Mouse Monoclonal
Kidney, Chromophobe Renal Cell Carcinoma
50 tests
IgG1
Kidney
Description
Kidney-specific cadherin (KSP-Cadherin) is a novel, kidney-specific member of the cadherin family of cell adhesion molecules. Within
the kidney, KSP-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and
not in glomeruli, renal interstitial cells or blood vessels. Different cadherins, including E-cadherin, cadherin-6 and N-cadherin, have
been investigated in renal cell cancers, demonstrating possible correlations of tumour dedifferentiation and the presence of lymph node
metastasis with loss of cadherins. Mazal, et al. investigated the utility of using KSP-Cadherin to distinguish chromophobe renal cell
carcinoma from oncocytoma. He found a membranous pattern of staining in 96% of 30 chromophobe carcinomas and in only 6% of 31
oncocytomas leading them to conclude that this is a useful antibody in differentiating these two lesions. Shen, et al., on the other hand,
found KSP-Cadherin positivity in 100% of 13 chromophobe RCCs and 95% of 20 oncocytomas.
References
1. Mazal PR, et al., Hum Pathol. 36(1):22-8, 2005.
2. Shen SS, et al., Mod Pathol. 18(7):933-40, 2005.
3. Thedieck C, et al., Br J Cancer. 92(11):2010-7, 2005.
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Lambda, CONFIRM
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
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NEXT>
760-2515 05267021001
Rabbit Polyclonal
Tonsil
Surface Membrane and/or Cytoplasmic
50 tests
Description
CONFIRM Lambda Rabbit Polyclonal Primary Antibody specifically binds to antigens located in the cell membrane and cytoplasmic
regions of normal B cells and plasma cells. Lambda light chains are polypeptide chains which in combination with heavy chains form
immunoglobulin molecules. There are two classes of light chains found in immunoglobulins, kappa light chains and lambda light chains.
Light chain production by lymphoid cells is genetically restricted such that the immunoglobulin molecules produced by an individual cell
will only contain a single light chain class. This clonal restriction may be used to indicate the polyclonal or monoclonal nature of B cell and
plasma cell populations. Because clonality is a clinically important attribute to aid in the subclassification of lymphomas, the identification
of light chain class has gained increasing importance in immunopathology.
References
1. Landaas TO, et al., Acta Path Microbiol Scand. 89(2):91-101, 1981.
2. Pangalis GA, et al., Cancer. 45(6):1334-1339, 1980.
LMO2 , CONFIRM
(1A9-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Woda BA, et al., Cancer. 43(1):303-307, 1979.
790-4368 05479274001
Mouse Monoclonal
Tonsil, Lymph Node
Nuclear
50 tests
IgG1/K
Normal tonsil
Description
CONFIRM LMO2 (1A9-1) Mouse Monoclonal Primary Antibody (IgG1) is directed against a 24 kD protein in the LIM only family. LMO2 is
expressed in normal germinal centre (GC) B cells and in a subset of GC derived B-cell lymphomas and normal hematopoietic lineages.
LMO2 is essential for the regulation of hematopoietic stem cells and is expressed in erythroid and myeloid precursors playing a crucial
role in hematopoietic development. Preliminary studies demonstrate LMO2 expression in GC-derived B-cell lymphomas such as follicular,
Burkitt, lymphocyte-predominant Hodgkin’s and diffuse large B-cell (DLBCL) neoplasms. LMO2 staining was absent in mature B-cell and
T-cell lymphomas and in classical Hodgkin’s lymphomas.
References
1. Natkunam Y, et al., Blood. 109:1636-1642, 2007.
2. Lossos I, et al., N Engl J Med. 350:1828-1837, 2004.
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Luteinizing Hormone (LH)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2802 05268222001
Rabbit Polyclonal
Normal Pituitary
Cytoplasmic
50 tests
Pituitary
Description
Luteinizing Hormone (LH) Rabbit Polyclonal Primary Antibody is a useful marker in classification of pituitary tumours and the study of
pituitary disease. It reacts with LH-producing cells (gonadotrophs).
References
2. Saccomanno K, et al., J Clin Endocrinol Metab. 78(5):1103-7, 1994.
3. Kovalitc JJ, J Neurooncol. 16(3):227-32, 1993.
Lysozyme
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
4. Felix I, et al., Hum Pathol. 22(7):179-21, 1991.
5. Sano T et al. Virchows Arch A Pathol Anat Histopathol.
1990;417(4):361-7.
760-2656 05267668001
Rabbit Polyclonal
Tonsil, Lymph Node
Cytoplasmic
50 tests
Tonsil
Description
Lysozyme Rabbit Polyclonal Primary Antibody stains myeloid cells, histiocytes, granulocytes, macrophages and monocytes in human tonsil,
colon and skin. It is an important marker that may demonstrate the myeloid or monocytic nature of acute leukaemia. The restrictive nature
of Lysozyme antibody staining suggests that lysozyme may be synthesised predominantly in reactive histiocytes rather than in resting,
unstimulated phagocytes. It has not been determined whether lysozyme stains any other cell or tissue type. Lysozyme antibody may aid in
the identification of histiocytic neoplasias, large lymphocytes and classifying lymphoproliferative disorders.
References
1. Morsky P, et al., Clin Chim Acta. 178:327-36, 1988.
2. Krugliak L, et al., Am J Hematol. 21:99-109, 1986.
3. Delaflor-Weiss E, et al., Acta Cytol. 43(6):1124-30, 1999.
4. Yuen ST, et al., Histopathology. 32(2):126-32, 1998.
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Macrophage
(HAM-56)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-2657 05267676001
Mouse Monoclonal
Tonsil, Lymph Node
Cytoplasmic
50 tests
IgM/K
Tonsil, macrophages
Description
Macrophage (HAM-56) Mouse Monoclonal Primary Antibody reacts with tingible macrophages, interdigitating macrophages of
lymph nodes and tissue macrophages, e.g. Kupffer cells of the liver and alveolar macrophages of the lung. The antibody also stains a
subpopulation of endothelial cells, most prominently those of the capillaries and smaller blood vessels. Macrophage antibody reacts with
monocytes, but is unreactive with B- and T-lymphocytes.
References
1. Gown AM, et al., Am J Pathol. 125:191-207, 1986.
2. Alpers CE, et al., Am J Clin Pathol. 92:662-665, 1989.
Mammaglobin
(31A5)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Bosman C, et al., J Pediatr Hematol Oncol. 21(1):31-7, 1999.
4. Soini Y, et al., Pathol Res Pract. 186(6):759-67, 1990.
760-4263 05269253001
Rabbit Monoclonal
Breast Carcinoma
Cytoplasmic
50 tests
IgM/K
Breast carcinoma
Description
Mammaglobin (10 kD) is a breast-associated glycoprotein distantly related to secretoglobin family that includes human uteroglobin and
lipophilin. Mammaglobin mRNA is present in high levels in human breast cancer cell lines, which has been shown to be a sensitive marker
of breast cancer. When combined with other breast-restricted markers such as GCDFP-15, an overall sensitivity for breast carcinoma of
84% has been achieved. Mammaglobin antibody can also be used to determine breast origin in the setting of metastatic carcinoma.
References
1. Watson MA, et al., Cancer Research. 59:3028-3031, 1999.
2. Fleming TP, et al., Ann N Y Acad Sci. 923:78- 89, 2000.
3. Han JH, et al., Arch Pathol Lab Med. 127:1330-1334, 2003.
4. Bhargava R, et al., Am J Clin Pathol. 127(1):103-13, 2007.
5. Sasak E, et al., Breast Mod Pathol. 20 (2):208-14. 2007.
6. Wang Z, et al., Int J Clin Exp Pathol. 2(4):384-9, 2009.
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MART-1/melan A, CONFIRM
(A103)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-2990 05278350001
Mouse Monoclonal
Normal Skin, Melanoma
Cytoplasmic
50 tests
IgG1
Melanoma
Description
Melanoma is a malignant tumour deriving from melanocytes. Melanocytes are derivative cells originating from the neuroectodermal crest,
which migrate during the early foetal period into the skin, where they settle within the epidermis and become part of the complex skin
structure. One of the most prominent functional features of melanocytes is to produce the melanin pigment as a response to UV radiation
in order to protect the skin structures from sun damage. Approximately 47,700 cases per year are diagnosed in the United States alone
and the incidence is increasing at the rate of 4.3% per year. In about 2% of occurrences, the disease is present even though no skin
discolouration occurs. The American Cancer Society estimates that there are currently 480,000 cases of melanoma in America today and
that there are 7,700 deaths per year. It has been reported that in the US, Europe and Australia there are 90,000 new cases diagnosed per
year and 15,000 deaths annually. To aid in the diagnosis of these potentially lethal lesions, monoclonal antibodies have been raised against
an antigenic determinate localised to a glycoprotein that is restricted to cells of melanocytic lineage. The molecule is located in the inner
membranes of type 1, 2 and 3 pre-melanosomes and serves as a potential target of cytotoxic T-lymphocytes, probably in concert with
MHC2A. MART-1/melan A, was named specifically for that property (melanoma antigen recognised by T cells-1) and is recognised by
T cells.
In practice, HMB-45 and MART-1/melan A have enjoyed the greatest use as agents to confirm the identity of polyclonal S100 positive
neoplasms as melanocytic in nature. MART-1/melan A and HMB-45 behave differently depending on the tissue fixation. MART-1/melan A
is suitable for formalin-fixed tissues and archival material which requires heat-induced epitope retrieval (HIER). Both HMB-45 and MART1/melan A show increased sensitivity with HIER, though HMB-45’s immunoreactivity is stronger in ethanol-fixed tissues than formalinfixed. Prolonged fixation in formalin significantly diminishes HMB-45’s immunoreactivity. HMB-45 is also vulnerable to a high degree of
non-specific staining when used with Mercury-based fixatives.
The two monoclonal antibodies also differ in their detection rates of melanoma. MART-1/melan A has demonstrated positive rates of 8190% versus HMB-45 at 75-80% on primary and metastatic malignant melanoma. Unlike HMB-45, MART-1/melan A is purported to yield
a homogeneous cytoplasmic staining pattern in both melanomas and melanocytic nevi, with a stronger intensity and greater percentage
of tumour cell immunopositivity. HMB-45 stains mainly the intraepidermal and superficial dermal component of compound nevi. MART-1/
melan A is useful for the detection of primary and metastatic melanomas, especially with an epithelioid morphology, yet it has a limited role
in the differential diagnosis of desmoplastic melanomas.
In a study published by AA Jungbluth, et al., additional information as to the detection rates of melanoma is as follows: of the 75 metastatic
melanomas tested 81% were A-103 positive and 75% were HMB-45 positive. Of the positive cases, A-103 showed homogeneous staining in
a significantly higher number of cases than HMB-45 (72% vs. 52%, respectively). Focal staining with less than 5% reactive tumour cells was
seen more frequently in HMB-45 (12 of 56) than in A-103 (5 of 61).
References
1. Dabbs DJ, et al., Immunohistochemistry, Churchill Livingstone,
Philadelphia, 2002.
2. Leong AS-Y, et al., Diagnostic Antibodies for Immunohistology, 2nd
Edition. Greenwich Medical Media Ltd., London, 2003.
3. Jungbluth AA, et al., Am J Surg Pathol. 22(5): 595-602, 1998.
4. Jungbluth AA, et al., Virchows Arch. 434:429-435, 1999.
140 NEXT>
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Melanoma Associated Antigen NEW
(KBA.62)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4527 06419186001
Mouse Monoclonal
Melanoma
Cytoplasmic
50 tests
IgG1
Description
Melanoma Associated Antigen (KBA.62) Mouse Monoclonal Primary Antibody is a novel melanoma antibody. Studies thus far have shown
a similar sensitivity to melanocytic proliferations as that seen with S-100 protein staining, which is somewhat higher than that seen with
HMB-45. This has been confirmed by one study on a series of 215 sentinel lymph nodes. Moreover, KBA.62 antibody identified 6 patients
(3%) who had confirmed sentinel lymph node metastasis but stained negative for HMB-45. In this setting, the resolution appears to be
higher than S-100 protein in that the staining pattern (membranous) is quite distinct.
References
1. Morris LG, et. al., Head Neck. 30:771-775, 2008.
2. Busam KJ, et. al., Am J Surg Pathol. 29: 400-406, 2005.
3. Rochaix P, et. al., Mod Pathol. 16:481-490, 2003.
4. Nonaka D, et. al., Am J Clin Pathol. 127:787-791, 2003.
5. Zhe X, et. al., J Histochem Cytochem. 52:1537-1542, 2004
Melanoma Associated Antigen NEW
(PNL2)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4528 06419194001
Mouse Monoclonal
Melanoma
Cytoplasmic
50 tests
IgG1
Description
Melanoma Associated Antigen (PNL2) Mouse Monoclonal Primary Antibody is a novel monoclonal antibody, which has recently been
introduced as an immunohistochemical reagent to stain melanocytes and tumours derived therefrom. PNL2 antibody yields a strong
cytoplasmic staining of skin and oral mucosal melanocytes and staining of granulocytes when used at high concentration. PNL2 antibody
labels intraepidermal nevi while the dermal component of compound nevi are largely non-reactive with PNL2 antibody. Antibodies against
PNL2, melan-A and HMB-45 stain most clear cell sarcoma cells and a few cells in angiomyolipomas and lymphangioleiomyomatosis. Nonmelanocytic lesions found to be positive with this marker include PEComas and melanotic schwannoma. PNL2 antibody is a useful antibody
for the identification of melanomas and clear cell sarcomas.
References
1. Shah RB, et. al., Am J Clin Pathol. 122:517-523, 2004.
2. Morris LG, et. al., Head Neck. 30:771-5, 2008.
3. Busam KJ, et. al., Am J Surg Pathol. 29: 400-6, 2005.
4. Rochaix P, et. al., PNL2, Mod Pathol. 16:481-90, 2003.
5. Nonaka D, et. al., Am J Clin Pathol. 127:787-91, 2007.
6. Zhe X, et. al., J Histochem Cytochem. 52:1537-42, 2004.
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<PREV
Melanosome, CONFIRM
(HMB45)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4366 05479282001
Mouse Monoclonal
Melanoma, Skin
Cytoplasmic
50 tests
IgG1/K
Melanoma, with area showing normal melanoctyes
Description
CONFIRM Melanosome (HMB45) Mouse Monoclonal Primary Antibody has been shown to react with immature melanosomes in foetal
and neonatal melanocytes and infantile retinal pigment epithelium. It does not react with normal resting adult melanocytes, regardless
of the degree of pigmentation. In malignant cells, HMB45 stains most primary and metastatic melanomas. Desmoplastic malignant
melanomas infrequently express the HMB45 antigen, possibly because they contain few melanosomes and have a mesenchymal pattern of
differentiation. Non-melanocytic tumour cells of epithelial, lymphoreticular, glial and mesenchymal origin are not labelled except for renal
angiomyolipomas (PAML), a type of mesenchymal hamartoma.
References
1. Kapur R, et al., J Histochem Cytochem. 40(2):207-212, 1992.
2. Gown A, et al., Am J Pathol. 123(2):195-203, 1986.
3. Esclamado R, et al., Am J Surg. 152(4): 376-385, 1986.
4. Colombari R, et al., Virchows Archiv A Pathol Anat. 413(1):17-24, 1988.
Mesothelial Cell
(HBME-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Skelton H, et al., Am J Dermapathol. 13(6): 543-550, 1991.
6. Ordonez N, et al., Am J Clin Pathol. 90(4): 385-390, 1988.
7. Pea M, et al., Pathol. 23(3): 185-188, 1991.
8. Ashfaq R, et al., Cancer. 71(10): 3091-3097, 1993.
760-4445 05973813001
Mouse Monoclonal
Mesothelioma, Thyroid Carcinoma
50 tests
IgM/K
Pleural biopsy, malignant mesothelioma
Description
Mesothelial Cell (HBME-1) Mouse Monoclonal Primary Antibody has been demonstrated to label mesothelial cells, both benign and
malignant (malignant mesothelioma) and thus has been used in distinguishing mesothelioma from adenocarcinomas of various origins.
More recently this antibody has been used to distinguish thyroid carcinomas (both follicular and papillary) from benign thyroid lesions.
References
1. Coli A, et al., J Exp Clin Cancer Res. 26(2):221-7, 2007.
2. Cabibi D, et al., Thyroid. 17(7):603-7, 2007.
3. Torregrossa L, et al., Hum Pathol. 38(10):1482-8, 2007.
4. Barroeta JE, et al., Endocr Pathol. 17(3):225-34, 2006.
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MITF, CONFIRM
(C5/D5)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
790-4367 05549175001
Mouse Monoclonal
Melanoma
Nuclear
50 tests
IgG1
Melanoma
Description
CONFIRM MITF (C5/D5) Mouse Monoclonal Primary Antibody is a mouse monoclonal cocktail. This antibody cocktail reagent detects
microphthalmia transcription factor (MITF) proteins 52-56 KD of MITF expressing cells in normal and neoplastic tissues. This antibody is
used in the identification of melanotic lesions such as malignant melanoma and melanotic neurofibroma.
References
1. Tachibana M, et al., Hum Mol Genet. 3(4):553, 1994.
2. Chang KL, et al., Adv Anat Pathol. 8(5):273, 2001.
3. Widlund HR, et al., Oncogene. 22(20):3035, 2003.
MLH-1 NEW
(M1)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. King R, et al., Am J Pathol. 155(3):731, 1999.
5. Granter SR, et al., Appl Immunohistochem Mol Morphol. 10(1):47,
2002.
6. Dorvault CC, et al., Cancer. 93(5):337, 2001.
790-4535 06472966001
Mouse Monoclonal
Colon, Breast, Appendix, Testis and Ovary
Nuclear
50 tests
IgG2a
Colon carcinoma
Description
MLH-1 (M1) Mouse Monoclonal Primary Antibody is used to qualitatively identify human DNA mismatch repair (MMR) protein MLH1,
expressed in the nucleus of normal proliferating cells. Deficient or low levels of MLH1 are associated with colorectal and other cancers.
References
1. Aaltonen LA, et al. N Engl J Med. 338:1481-1487, 1998.
2. Boyer J, et al. Cancer Res. 55:6063-6070, 1995.
3. Lawes, D, et al. Br J Cancer. 93:472-477, 2005.
4. Chen Y, et al. Cancer Res. 61(10):4112-2, 2001.
5. Chen Y, et al. Cancer Res. 61(10):4112-2, 2001.
6. Caldés, T. Oncol Rep. 12:621-629, 2004.
7. Ellis NA. Am J Surg Pathol. Jan 29(1):96-104, 2005.
8. Berends MJW, et al. Am J Hum Genet. 70:26-37, 2002.
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MSH2
(G219-1129)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4265 05269270001
Mouse Monoclonal
Colon Mucosa, Colon Carcinoma
Nuclear
50 tests
IgG1
Colon
Description
MSH2 is a mismatch repair gene which is deficient in a high proportion of patients with microsatellite instability (MSI-H). This finding is
associated with the autosomal dominant condition known as Hereditary Non-Polyposis Colorectal Cancer (HNPCC). MSH2 (G219-1129)
Mouse Monoclonal Primary Antibody is useful in screening patients and families for this condition. Colon cancers that are microsatellite
unstable have a better prognosis than their microsatellite stable counterparts.
References
1. Wright CL, et al., Am J Surg Pathol. 27: 1393-1406, 2003.
2. Brueckl WM, et al., Anticancer Res. 23:1773-1778, 2003.
3. Rigau V, et al., Arch Pathol Lab Med. 127:694-700, 2003.
4. Renkonen E, et al., J Clin Oncol. 21:3629-3637, 2003.
MSH6, CONFIRM
(44)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Hoedema R, et al., The Am Surgeon. 69(5):387-92, 2003.
6. Christensen M, et al., Cancer. 95:2422-30, 2002.
7. Wahlberg SS, et al., Cancer Res. 62:3485-3492, 2002.
8. Lanza G, et al., Modern Pathol. 15:741-749, 2002.
790-4455 05929911001
Mouse Monoclonal
Colon Carcinoma, Breast Carcinoma
Nuclear
50 tests
IgG1
Gastric, normal
Description
CONFIRM MSH6 (44) Mouse Monoclonal Primary Antibody is used to qualitatively identify human DNA mismatch repair (MMR) protein
MSH6, expressed in the nucleus of normal proliferating cells. Deficient or low levels of MSH6 are associated with colorectal and other
cancers.
References
1. Lawes DA, Br J Cancer. 93:472-477, 2005.
2. Miyaki M, Nat Genet. 17:271-272, 1997.
3. Offit K, J Clin Oncol. 22:4449-4451, 2004.
4. Acharya S, et al., Proc Natl Acad Sci USA. 93:13629-13634, 1996.
5. Guerrette S, et al., Mol Cell Biol. 18:6616-6623, 1996.
6. Aaltonen LA, et al., N Engl J Med. 338:1481-1487, 1998.
7. Caldés, T. et al., Oncol Rep. 12:621-629, 2004.
8. Ellis NA. Am J Surg Pathol. 29(1):96-104, 2005.
9. Berends MJW, et al., Am J Hum Genet. 70:26-37, 2002.
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MUC1 NEW
(H23)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
790-4574 06316514001
Mouse Monoclonal
Breast, Lung, Pancreas
50 tests
IgG
Lung adenocarcinoma
Description
MUC1 (H23) Mouse Monoclonal Primary Antibody is directed against the membrane bound, glycosylated phosphoprotein MUC1. This
antibody may be used to aid in the identification of normal and neoplastic MUC1 expressing cells.
References
1. Guddo F, et al. J Clin Pathol. 51:667-71, 1998.
2. Guddo F, et al. Anticancer Res. 18:1915-20, 1998.
MUC2
(MRQ-18)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Barratt-Boyes S. Cancer Immunol Immunother. 43:142–151, 1996.
4. Taylor-Papadimitriou J, et al. Trends Biotechnol. 12:227–233, 1994.
760-4388 05463556001
Mouse Monoclonal
Colon, Colon Adenocarcinoma
Cytoplasmic
50 tests
IgG1/K
Colon
Description
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric epithelium
from chemical and mechanical aggressions. In humans, at least 14 mucin genes have been identified that code for the mucin proteins. They
are designated as MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC6 , MUC7, MUC8, MUC9, MUC11, MUC12, MUC13 and MUC16.
Mucins share a common feature of a tandem-repeat domain rich in serine and threonine residues. These amino acid residues are potential
O-glycosylation sites for attachment of the O-glycan chains that constitute up to 80% of the molecular weight of the final mucin glycoprotein.
The heterogeneous pattern of mucin expression, including the expression of the intestinal mucin MUC2, may provide new insights into the
differentiation pathways of gastric carcinoma. The pattern of mucin expression may also be used as a clue to bring new insights into the biological
behaviour of distinct clinicopathological entities related to the localisation of gastric carcinoma, namely proximal and distal gastric carcinomas.
Pinto-de-Sousa et al. have shown in a comprehensive study of gastric carcinomas evaluated for expression of several mucins (MUC1, MUC2,
MUC5AC and MUC6) that: (1) mucin expression is associated with tumour type (MUC5AC with diffuse and infiltrative carcinomas and MUC2 with
mucinous carcinomas) but not with the clinico-biological behaviour of the tumours; and (2) mucin expression is associated with tumour location
(MUC5AC with antrum carcinomas and MUC2 with cardia carcinomas), indirectly reflecting differences in tumour differentiation according
to tumour location. The following generalities apply to the patterns of Mucin expression. MUC1 expression: apical surfaces of most epithelial
cells in breast, gastro-intestinal (GI), respiratory and genitourinary (GU) tracts. MUC2 expression: specifically expressed in goblet cells of the
small intestine & colon. Colonic carcinomas – 65%, Gastric carcinomas – 42%, Oesophageal carcinomas – 17%. Rare outside of GI tract – with
exception of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
References
1. Chaves P, et al., Dis Oesophagus. 18(6):383-7, 2005.
2. Leteurtre E, et al., World J Gastroenterol. 12(21):3324-31, 2006.
3. Mino-Kenudson M, et al., Arch Pathol Lab Med. 131(1):86-90, 2007.
4. Mizoshita T, et al., Histol Histopathol. 22(3):251-60, 2007.
5. O’Connell FP, et al., Arch Pathol Lab Med. 129(3):338-47, 2005.
6. Park SY, et al., Arch Pathol Lab Med. 131(10):1561-7, 2007.
7. Rakha EA, et al., Mod Pathol. 18(10):1295-304, 2005.
HOME
MUC5AC
(MRQ-19)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4389 05463564001
Mouse Monoclonal
Stomach, Associated Adenocarcinomas
Cytoplasmic
50 tests
IgG1
Stomach
Description
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric
epithelium from chemical and mechanical aggressions. In humans, at least 14 mucin genes have been identified that code for the mucin
proteins. They are designated as MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC6 , MUC7, MUC8, MUC9, MUC11, MUC12, MUC13
and MUC16. Mucin genes are expressed in a regulated cell- and tissue-specific manner. The stomach provides a good example of such
differential expression of mucin genes. MUC1 is detected in mucous cells of the surface epithelium and neck region of the gastric antrum,
as well as in pyloric glands and oxynthic glands of the body region. MUC5AC is highly expressed in foveolar epithelium of both body
and antrum, whereas MUC6 protein expression is limited to mucous neck cells of the body and pyloric glands of the antrum. The mucin
expression pattern of gastric carcinoma is heterogeneous. It includes mucins normally expressed in gastric mucosa (MUC1, MUC5AC and
MUC6) and de novo expression of the intestinal mucin MUC2. The heterogeneous pattern of mucin expression, including the expression
of the intestinal mucin MUC2, may provide new insights into the differentiation pathways of gastric carcinoma. Pinto-de-Sousa, et al. have
shown in a comprehensive study of gastric carcinomas evaluated for expression of several mucins (MUC1, MUC2, MUC5AC and MUC6)
that: (1) mucin expression is associated with tumour type (MUC5AC with diffuse and infiltrative carcinomas and MUC2 with mucinous
carcinomas) but not with the clinico-biological behaviour of the tumours; and (2) mucin expression is associated with tumour location
(MUC5AC with antrum carcinomas and MUC2 with cardia carcinomas), indirectly reflecting differences in tumour differentiation according
to tumour location.
The following generalities apply to the patterns of Mucin expression:
MUC5AC expression:
• preferentially expressed in the normal stomach and respiratory
tract
• GI & pancreatobiliary ACAs
• Oesophageal CAs (67%)
• Gastric CAs (58%)
• Colonic CAs (6-25%)
• Pancreatic ductal CAs (73%)
• Cholangiocarcinomas (45%)
• Endocervical ACAs (70%)
• Endometrial ACAs (22%)
• Lung ACAs (14%) MUC1+/MUC2-/MUC5AC+ pattern:
• Endocervical adenoCAs (50%)
• Pancreatic ductal adenoCAs (64%) MUC1+/MUC2-/MUC5ACpattern
• Ductal and lobular breast CAs (100%)
• Urothelial CAs (bladder) (93%)
• Renal CAs (75%)
• Ovarian ACAs of various types
• Most pulmonary adenocarcinomas (81%)
• Endometrial (78%) adenoCAs (small subset expresses MUC5AC)
MUC1-/MUC2-/MUC5AC- pattern
• Hepatocellular CA
• Adrenocortical CA
• Prostate CA
References
146 NEXT>
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MUC6
(MRQ-20)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4390 05463572001
Mouse Monoclonal
Stomach, Associated Adenocarcinomas
Cytoplasmic
50 tests
IgG1
Stomach
Description
Mucins are high molecular weight glycoproteins which constitute the major component of the mucus layer that protects the gastric
epithelium from chemical and mechanical aggressions. In humans, at least 14 mucin genes have been identified that code for the mucin
proteins. They are designated as MUC1, MUC2, MUC3, MUC4, MUC5AC, MUC5B, MUC6 , MUC7, MUC8, MUC9, MUC11, MUC12, MUC13
and MUC16.
Mucin genes are expressed in a regulated cell- and tissue-specific manner. The stomach provides a good example of such differential
expression of mucin genes. MUC1 is detected in mucous cells of the surface epithelium and neck region of the gastric antrum, as well as
in pyloric glands and oxynthic glands of the body region. MUC5AC is highly expressed in foveolar epithelium of both body and antrum,
whereas MUC6 protein expression is limited to mucous neck cells of the body and pyloric glands of the antrum. The mucin expression
pattern of gastric carcinoma is heterogeneous. It includes mucins normally expressed in gastric mucosa (MUC1, MUC5AC and MUC6) and
de novo expression of the intestinal mucin MUC2.
The heterogeneous pattern of mucin expression, including the expression of the intestinal mucin MUC2, may provide new insights into the
differentiation pathways of gastric carcinoma. Pinto-de-Sousa, et al. have shown in a comprehensive study of gastric carcinomas evaluated
for expression of several mucins (MUC1, MUC2, MUC5AC and MUC6) that: (1) mucin expression is associated with tumour type (MUC5AC
with diffuse and infiltrative carcinomas and MUC2 with mucinous carcinomas) but not with the clinico-biological behaviour of the tumours;
and (2) mucin expression is associated with tumour location (MUC5AC with antrum carcinomas and MUC2 with cardia carcinomas),
indirectly reflecting differences in tumour differentiation according to tumour location.
The following generalities apply to the patterns of Mucin expression:
MUC5AC expression:
• preferentially expressed in the normal stomach and respiratory
tract
• GI & pancreatobiliary ACAs
• Esophageal CAs (67%)
• Gastric CAs (58%)
• Colonic CAs (6-25%)
• Pancreatic ductal CAs (73%)
• Cholangiocarcinomas (45%)
• Endocervical ACAs (70%)
• Endometrial ACAs (22%)
• Lung ACAs (14%) MUC1+/MUC2-/MUC5AC+ pattern:
• Endocervical adenoCAs (50%)
• Pancreatic ductal adenoCAs (64%) MUC1+/MUC2-/MUC5ACpattern
• Ductal and lobular breast CAs (100%)
• Urothelial CAs (bladder) (93%)
• Renal CAs (75%)
• Ovarian ACAs of various types
• Most pulmonary adenocarcinomas (81%)
• Endometrial (78%) adenoCAs (small subset expresses MUC5AC)
• MUC1-/MUC2-/MUC5AC- pattern
• Hepatocellular CA
• Adrenocortical CA
• Prostate CA
References
NEXT>
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MUM1 NEW
(MRQ-43)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4529 06419208001
Rabbit Monoclonal
Tonsil
50 tests
IgG1
Description
MUM1 is a 50 kD protein encoded by MUM1 gene and a member of the interferon regulatory factor family of transcription factors. MUM1/
IRF4 is expressed in the nuclei and cytoplasm of plasma cells and a small percentage of germinal centre (GC) B cells located in the “light
zone.” This antibody stains MUM1 protein, which is expressed in a subset of B cells in the light zone of the germinal centre, plasma cells,
activated T cells and a wide spectrum of related hematolymphoid neoplasms derived from these cells. Therefore, this antibody is useful
for the subclassification of lymphoid malignancies and an excellent marker for Hodgkin’s and Reed-Sternberg cells of classic Hodgkin’s
disease in combination with CD30 antibody.
References
1. Falini B, et. al., Blood. 95:2084-92, 2000.
2. Grossman A, et. al., Genomics. 37:229-33, 1996.
3. Neresh KN, Hematologica. 92:267-8, 2007.
4. Van Imhoff GW, et. al., J Clin Oncol. 34:4135-42, 2006.
5. Gualco G, et. al., Appl Immunohistochem Mol Morphol. 18:30110, 2010.
Myeloperoxidase (MPO)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2659 05267692001
Rabbit Polyclonal
Bone Marrow
Cytoplasmic
50 tests
Peripheral blood, granulocytes
Description
Myeloperoxidase Rabbit Polyclonal Primary Antibody detects granulocytes and monocytes in blood and precursors of granulocytes in the
bone marrow. This antibody can detect myeloid cell populations of the bone marrow as well as in other sites.
References
1. Pinkus GS, et al., Mod Pathol. 4(6): 733-41, 1991.
2. Hudock J, et al., Am J Clin Pathol. 102(1): 55-60, 1994.
3. Hamoudi WH, et al., Arch Pathol Lab Med. 124(2):315-8, 2000.
148 NEXT>
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Myogenin
(F5D)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-2832 05268290001
Mouse Monoclonal
Rhabdomyosarcoma
Nuclear
50 tests
IgG1/K
Rhabdomyosarcoma
Description
Myogenin (F5D) Mouse Monoclonal Primary Antibody labels the nuclei of myoblasts in developing muscle tissue and is expressed in
tumour cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilm’s tumour.
References
1. Miller JB, et al., J Cell Biol. 111(3):1149-59, 1990.
2. Wang NP, et al., Am J Pathol. 147(6):1799-810, 1995.
Myoglobin
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
3. Cui S, et al., Pathol Int. 49(1):62-8, 1999.
4. Cessna MH, et al., Am J Surg Pathol. 25(9):1150-7, 2001.
760-2660 05267706001
Rabbit Polyclonal
Skeletal Muscle
Cytoplasmic
50 tests
Skeletal muscle with degenerative changes
Description
Myoglobin Rabbit Polyclonal Primary Antibody is useful for the identification of tumours of muscle origin. Since myoglobin is found
exclusively in skeletal and cardiac muscle and is not present in any other cells of the human body, it may be used to distinguish
rhabdomyosarcoma from other soft tissue tumours. Myoglobin antibody staining is also useful when demonstrating rhabdomyoblastic
differentiation in other tumours, e.g. neurogenic sarcomas and malignant mixed mesodermal tumours of the uterus and ovary.
References
1. Mukai K, et al., Am J Surg Pathol. 3:373-376, 1979.
2. Corson JM, et al., Am J Pathol. 103:384-389, 1981.
3. Kindblom LG, et al., Acta Pathol Miro. 1982; SCand C90(Sec A):167174.
4. Brooks JJ, et al., Cancer. 50:1757-1763, 1982.
5. Kahn HJ, et al., Cancer. 50:1897-1903, 1983.
HOME
<PREV
Myosin, Smooth Muscle
(SMMS-1)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2704 05268133001
Mouse Monoclonal
Intestine, Breast
Cytoplasmic
50 tests
IgG1/K
Vascular smooth muscle
Description
Smooth Muscle Myosin, heavy chain (SMMS-1) is a cytoplasmic structural protein that is a major component of the contractile apparatus
of the smooth muscle cells. SMMS-1 is also a myoepithelium-associated protein. SMMS-1 Mouse Monoclonal Primary Antibody is
an antibody to smooth muscle myosin, heavy chain that reacts with human visceral and vascular smooth muscle cells. The antibody
also reacts with human myoepithelial cells. It is very helpful in distinguishing between benign sclerosing breast lesions and infiltrating
carcinomas in difficult cases since it strongly stains the myoepithelial layer in the benign lesions while it is negative in the infiltrating
carcinomas.
References
1. Nan Ping Wang, et al., Appl Immunohistochem. 5(3):141-151.
2. Werling RW, et al., Am J Surg Pathol. 27(1):82-90, 2003.
3. Dabbs DJ, et al., Diagn Cytopathol. 20(4):203-7, 1999.
Napsin A
(Polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Agoff SN, et al., Appl Immunohistochem Mol Morphol. 9(2):1649, 2001.
760-4446 05973805001
Rabbit Polyclonal
Lung Adenocarcinoma, Kidney
Cytoplasmic
50 tests
IgG
Adenocarcinoma of lung
Description
Napsin is a pepsin-like aspartic proteinase in the A1 clan of the AA clade of proteinases. There are two closely related napsins, napsin A
and napsin B. Napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kD. Immunohistochemical
studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in
type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to
distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
References
1. Tatnell PJ, et al., FEBS Lett. 441(1):43-48, 1998.
2. Schauer-Vukasinovic V, et al., FEBS Lett. 462(1):135-9, 1999.
3. Hirano T, et al., J Cancer Res. 91(10):1015-1021, 2000.
4. Dejmek A, et al., Diagnostic Cytopathology. 35(8):493-7, 2007.
5. Jagirdar J, et al., Arch Pathol Lab Med. 132(3):384-96, 2008.
6. Suzuki A, et al., Pathol Res Pract. 201(8-9):579-86, 2005.
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Negative Control Mouse Ig
(MOPC21)
Part Number
Type
Quantity
<PREV
NEXT>
760-2014 05266670001
Mouse Monoclonal
250 tests
Description
Negative Control Mouse Ig is not directed against a known epitope present in human tissue. The reagent is intended for use as a negative
control in qualitative staining of sections of routine formalin-fixed, paraffin-embedded tissue on a VENTANA automated slide stainer.
Negative Control Rabbit Ig, CONFIRM
Part Number
Type
Control
Quantity
760-1029 05266238001
Rabbit Polyclonal
Tonsil, Lymph Node
250 tests
Description
CONFIRM Negative Control Rabbit Ig is purified rabbit Ig which is not directed against a known epitope present in human tissue. The
reagent is intended for use as a negative control for qualitative assessment of background staining for rabbit primary antibodies.
Nerve Growth Factor Receptor (NGFR)
(MRQ-21)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4391 05463599001
Mouse Monoclonal
Neurons, Ganglia, Malignant Melanoma Cells, Myoepithelial Cells
of Breast Ducts
Cytoplasmic
50 tests
IgG1
Description
NGFR, a 75 kd glycoprotein (also known as P-75NTR), is the first of neurotrophin receptors to be isolated and is a member of the tumour
necrosis factor (TNF) receptor family. It is expressed not only in sympathetic and sensory neurons, but also in various neural crest cell or
tumour derivatives such as melanocytes, melanomas, neuroblastomas, pheochromocytomas, neurofibromas and neurotised nevi (type C
melanocytes). NGFR has been shown to be a reliable marker for desmoplastic and neurotropic melanoma by several groups. Although the
sensitivity of NGFR staining in desmoplastic melanomas has not been systematically analysed, all reported cases stain with NGFR. A review
of 9 cases of desmoplastic melanomas diagnosed in the Dermatopathology Section of the Department of Dermatology at Boston University
School of Medicine between 2001 and 2004 disclosed that all 9 cases of desmoplastic melanomas tested stained positively with NGFR.
These findings are in contrast to a study reported by Huttenbach, et al in which only 33% of their desmoplastic melanomas stained with
NGFR. This difference emphasises the importance of the choice of monoclonal antibody to obtain consistent results. This staining property
of desmoplastic melanoma cells can be useful in diagnosing challenging cases, namely early lesions, suspected cases with negative or
weak S-100 staining and those that must be distinguished from a scar.
It is now apparent that expression of NGFR is ubiquitous and not limited to the nervous system, being expressed in mature non-neural cells
such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate
basal cells and myoepithelial cells. Unlike the high-affinity nerve growth factor tyrosine kinase receptors (TrkA, TrkB and TrkC), NGFR
has no intrinsic tyrosine kinase activity. Studies in prostate and urothelial cancer suggest that NGFR may act as a tumour suppressor,
negatively regulating cell growth and proliferation. NGFR antibody labels the myoepithelial cells of breast ducts and intralobular fibroblasts
of breast ducts and thus aids in the diagnosis of malignancy in the breast.
References
1. Laskin WB, et al., Hum Pathol. 31(10):1230-41, 2000.
2. Lewis Kelso R, et al., Dermatol Surg. 32(2):177-83, 2006.
3. Liang Y, et al., J Invest Dermatol. 111(1):114-8, 1998.
4. Liang Y, et al., J Cutan Pathol. 25(4):189-98, 1998.
5. Liang Y, et al., Arch Dermatol Res. 291(1):14-21, 1999.
HOME
Neurofilament
(2F11)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-2661 05267714001
Mouse Monoclonal
Intestine, Breast
Cytoplasmic
50 tests
IgG1/K
Cerebellum
Description
Neurofilament (2F11) Mouse Monoclonal Primary Antibody stains an antigen localised in a number of neural, neuroendocrine and
endocrine tumours. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for
neurofilament. Neurofilaments are also present in paragangliomas, adrenal and extra-adrenal pheochromocytomas. Carcinoids,
neuroendocrine carcinomas of the skin and oat cell carcinomas of the lung also express neurofilament.
References
1. Wood JN, et al., Biosci Rep, 1:263, 1981.
2. Anderton BH, et al., Nature. 298:84, 1982.
3. Miettinen M, et al., Lab Invest. 52:429-436, 1985.
4. Van Muijen GNP, et al., Am J Pathol. 116:363-369, 1984.
5. Trojanowski JQ, et al., N Eng J Med. 313:101-104, 1985.
6. Morrison CD, et al., Semin Diagn Pathol. 17(3):204-15, 2000.
7. Dubovy SR, et al., Br J Opthalmol.85(8):949-51, 2001.
8. Erana-Rojas IE, et al. Ann Diagn Pathol. 6(5):265-71, 2002.
NSE (Neuron Specific Enolase)
(E27)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2662 05267722001
Mouse Monoclonal
Pancreas
Cytoplasmic
50 tests
IgG1
Description
Neuron Specific Enolase is present in high concentration in neurons and in central and peripheral neuroendocrine cells, therefore, NSE
(E27) Mouse Monoclonal Primary Antibody reacts with cells of neural and neuroendocrine lineage. If neoplastic cells co-express keratins
and NSE, neuroendocrine differentiation is probable, however, neural tumours that do not express keratin and show no staining with NSE
would not exclude neural or neuroendocrine differentiation. Thus, detection of neural and neuroendocrine lineage requires the use of
panels that include NSE and other markers such as keratin, chromogranin, synaptophysin and neurofilament.
References
1. Perentes E, et al., Arch Pathol Lab Med. 111:796-812. 1987.
2. Cras P, et al., Ann Neurol. 20:106-17, 1986.
3. Schmechal D, et al., Lab Invest. 52:239-242, 1985.
4. Dhillon AP, et al., Histopathology. 6:81-92, 1982.
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Oct-2
(MRQ-2)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4447 05973791001
Mouse Monoclonal
Lymph Node, Tonsil
Nuclear
50 tests
IgG1/K
Description
Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes.
These are involved in proliferation and differentiation and despite the scarce evidence for Oct-2 expression in T cells, it has been shown
that this factor participates in transcriptional regulation during T-cell activation. Oct-2 activity is dependent on phosphorylation and
alternative splicing, although it seems that the level of its expression can be used as a marker of B-cell lineage and differentiation. The
following show high levels of Oct-2 expression: germinal centre B-cells, mantle B-cells, monocytoid B-cells and plasma cells. Various
lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukaemia, mantle cell lymphoma, follicular
lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt’s lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell
rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin’s lymphoma, classic Hodgkin’s lymphoma.
Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic and epithelial cell lines, whereas
all mature B-cell lines display high levels of expression. Analysis of Oct-2 expression in primary Hodgkin’s lymphoma (HLs) and cell lines
derived from it showed that the tumour cells analysed had high levels of expression and activity, suggesting a common B cell origin for all
types of HLs.
References
1. Browne P, et al., Am J Clin Pathol. 120(5):767-77, 2003.
2. García-Cosío M, et al., Mod Pathol. 17(12):1531-8, 2004.
3. Gibson SE, et al., Am J Clin Pathol. 126(6):916-24, 2006.
4. Hallermann C, et al., J Am Acad Dermatol. 56(4):588-97, 2007.
HOME
Oct-4
(MRQ-10)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4392 05463602001
Mouse Monoclonal
Seminoma, Dysgerminoma
Nuclear
50 tests
IgG
Seminoma
Description
OCT-4 is a transcription factor that maintains and regulates pluripotency in embryonic stem and germ cells. It has a very high sensitivity
and specificity in seminoma/dysgerminoma and embryonal carcinoma and in the germ cell component of gonadoblastoma by nuclear
immunostaining. Clear cell carcinoma may enter the differential diagnosis of dysgerminoma as both may grow in nests or tubules, contain
clear cells and have a prominent inflammatory infiltrate (lymphocytes in dysgerminoma and plasma cells in clear cell carcinoma). In
one study that looked at OCT-4 staining in clear cell carcinomas, 4 of 14 tumours were found to be focally positive. In another study, 49
endometrioid carcinomas were OCT-4 negative. Rarely dysgerminoma may have a morphologic appearance that overlaps with sex cordstromal tumours, expecially poorly differentiated Sertoli cell tumours. In two studies, however, all sex cord-stromal tumours of the testis
and granulose cell tumours of the ovary were OCT-4 negative.
References
4. Cools M, et al., J Clin Endocrinol Metab. 91(6):2404-13, 2006.
1. Baker PM, et al., Int J Gynecol Pathol. 24(1):39-55, 2005. Review.
Epub 2006 Apr 11.
2. Biermann K, et al., Histopathology. 49(3):290-7, 2006.
3. Cheng CJ, et al., J Biomed Sci. 14(6):797-807, 2007. Epub 2007 Aug 8. 5. Gidekel S, et al., Cancer Cell. 4(5):361-70, 2003.
p21WAF1
(DCS-60.2)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4453 05973961001
Mouse Monoclonal
Non-Small Cell Lung Carcinoma, Colon
Nuclear
50 tests
IgG2a
Colon mucosa, hyperplastic polyp
Description
p21 is a nuclear 21kD protein, a product of the WAF1/CIP1 gene. It is a cyclin-dependent kinase inhibitor 1A (p21, Cip1), also known as
CDKN1A, which in humans is encoded by the CDKN1A gene located on chromosome 6p21.2. It is a constituent of a large complex of
nuclear proteins, including cyclins, cyclin dependent kinases and PCNA (Proliferating Cell Nuclear Antigen). Cell cycle progression is
regulated by cyclins and their cognate Cdks. p21 inhibits the activity of each member of the cyclin/Cdk family. The expression of this gene
acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumour suppressor protein p53. Normal cells generally
display a rather intense nuclear p21 expression. Loss of p21 expression has been associated with poor prognosis in several carcinomas
(gastric carcinoma, non-small cell lung carcinoma and thyroid carcinoma).
References
1. DiGiuseppe JA, et al., Am J Pathol. 147(4):884-8, 1995.
2. Gomyo Y, et al., Cancer. 79(11):2067-2072, 1997.
3. Ikeguchi M, et al., Dig Dis Sci. 43(5):964-70, 1998.
4. Marone M, et al., Leuk Lymphoma. 43(1):51-7, 2003.
5. Kwon MS, et al., Pathol Res Pract. 202(12):849-56, 2006.
6. Merola E, et al., J Cell Physi. 207(2):512-519, 2006.
154 NEXT>
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p27kip1
(SX53G8)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4268 05269300001
Mouse Monoclonal
Non-Small Cell Lung Carcinoma, Colon
Cytoplasmic
50 tests
IgG1/K
Lymph node
Description
p27 is a cyclin-dependent kinase inhibitor which regulates progression from G1 to S phase by binding and inhibiting cyclin-dependent
kinases. Low p27 expression has been associated with unfavourable prognosis in renal cell carcinoma, colon carcinoma, small breast
carcinoma, non-small cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, lymph node metastases in papillary carcinoma of
the thyroid and more aggressive phenotype in a carcinoma of the cervix.
References
1. Lloyd RV, et al., Am J Pathol. 154:313-323, 1999.
2. Migita T, et al., Cancer. 94:973-9, 2002.
3. Haitel A, et al., Urology. 58:477-481, 2001.
p53
(Bp-53-11)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Tan P, et al., Cancer Research. 57-1259-1263, 1997.
5. Esposito V, et al., Cancer Research. 57:3381-3385, 1997.
6. Khoo MLC, et al., J Clin Endocrinol Metab. 87:1814-1818, 2002.
760-2542 05267102001
Mouse Monoclonal
Nuclear
50 tests
IgG2a
Bladder transitional cell carcinoma
Description
p53 (Bp53-11) Mouse Monoclonal Primary Antibody is directed against both the mutant and wild type of the p53 nuclear phosphoprotein.
Very rare normal cells express p53, but alterations in the p53 suppressor gene result in an overproduction of this protein in malignancies.
This reagent may be used to aid in the identification of abnormally proliferating cells in neoplastic cell populations. p53 (Bp53-11) antibody
specifically binds to nuclear antigen(s) associated with the normal down regulation of cell division. Increased expression of p53 in actively
dividing cells is an indication of loss of function due to mutation of the p53 gene.
References
1. Mayall FG, et al., J Pathol. 168:377-381, 1992.
2. Van den Berg FM, et al., Am J Pathol. 142:381-385, 1993.
HOME
p53, CONFIRM
(DO-7)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
800-2912 05278775001
Mouse Monoclonal
Nuclear
50 tests
IgG1/K
Colon carcinoma
Description
The wild type p53 molecule has a role in transcription, in cell cycle control and in many other metabolic functions. It is also a tumour
suppressor in that it halts abnormal growth in normal cells; however, a mutation in one of its 393 amino acids can eliminate the surveillance
capability of the protein and allow expression of malignant phenotype. Approximately 50% of all human cancers contain a mutation in p53.
The wild type molecule has a short half life, is present in low concentrations and cannot usually be detected by IHC. Many of the mutations
in p53 increase its half life and cause intranuclear accumulation of the mutant protein. Thus in many malignancies of the colon, stomach,
bladder, breast, lung, testes and in melanomas and soft tissue sarcomas, p53 protein can be readily detected in the malignant cells by IHC.
Immunostaining closely correlates with the presence of a mutation. The staining pattern with the CONFIRM p53 (DO-7) Mouse Monoclonal
Primary Antibody is exclusively nuclear and may be present in a heterogeneous pattern.
References
1. Mayall FG, et al., J Pathol. 168:377-381, 1992.
p63
(4A4)
Part Number
Type
Control
Localisation
Quantity
Isotype
2. Van den Berg FM, et al., Am J Pathol. 142:381-385, 1993.
790-4509 05867061001
Mouse Monoclonal
Prostate, Tonsil
Nuclear
50 tests
IgG2a/K
Prostate
Description
VENTANA p63 (4A4) Mouse Monoclonal Primary Antibody is directed against the p63 molecule which is highly expressed in the nuclei of
human prostatic basal cells and urothelial tissues. This antibody may be used to aid in the differentiation of benign and malignant prostatic
lesions.
References
1. Yang A, et al., Mol Cell. 2:305-16, 1998.
2. Grisanzio C, et al., J Cell Biochem. 103:1354-1368, 2008.
3. Yang A, et al., Nature. 398:714-718, 1999.
4. Signoretti S, et al., J Pathol. 157:1769-1775, 2000.
5. Wu HH, et al., Appl Immunohistochem Mol Morph. 12:285-289,
2004.
6. Shah RB, et al., Am J Surg Pathol. 26:1161-1168, 2002.
7. Weinstein MH, et al., Mod Pathol. 15:1302-1308, 2002.
8. Harton AM et al., Am J Clin Pathol. 128:80-85, 2007
156 NEXT>
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p120 NEW
(98)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
790-4517 05867088001
Mouse Monoclonal
Tonsil
Membranous (ductal), Cytoplasmic (lobular)
50 tests
IgG1
Description
p120 catenin (98) Mouse Monoclonal Primary Antibody is directed against human juxtamembrane protein p120 expressed as a part of the
cell-cell adhesion complex in epithelial tissues. Cytoplasmic accumulation of p120 is associated with lobular breast carcinoma, whereas
ductal neoplasms retain membranous localisation.
References
1. Dabbs DJ, et al., Am J Surg Pathol. 31(3):427-37, 2007.
2. Anastasiadis PZ., Biochimica et Biophysica Acta. 1773:34-46, 2007.
3. Mastracci TL, et al., Modern Pathology. 18:741-751, 2005.
4. Thoreson MA, et al., Differentiation. 70:583-589, 2002.
5. Thoreson MA, et al., J of Cell Bio. 148(1):189-201, 2000.
Parathyroid Hormone (PTH) NEW
(MRQ-31)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4532 06419224001
Mouse Monoclonal
Parathyroid
50 tests
IgG2a
Description
PTH (MRQ-31) Mouse Monoclonal Primary Antibody is useful to distinguish parathyroid hyperplasia/neoplasms from thyroid and
metastatic neoplasms; although the pathologist is typically aware of the pre-operative hypercalcaemic status. Occasionally when the
surgeon does not supply this information PTH immunohistochemistry is essential. Even more problematic are situations in which clear cell
parathyroid carcinomas are non-secretory without an abnormality in mineral metabolism.
References
1. Brown E, Mineral Electrolyte Metal. 8:130-50, 1982.
2. Chen HL, et. al. Journal of Biology and Chemistry. 277:19374-81,
2002.
3. Wick MR, et. al., Seminars in Diagnostic Pathology. 14:183-202, 1997.
4. Permanetter W, et. al., American Journal of Surgical Pathology.
7:535-46, 1983.
5. Aldinger KA, et. al., Cancer. 49:388-97, 1982.
6. Murphy MN, et. al. Cancer. 58:2468-76, 1986.
HOME
PAX5, CONFIRM
(SP34)
Part Number
Type
Control
Localisation
Quantity
<PREV
790-4420 05552729001
Rabbit Monoclonal
Tonsil, Spleen
Nuclear
50 tests
Lymphoma
Description
CONFIRM PAX5 (SP34) Rabbit Monoclonal Primary Antibody is directed against a nuclear epitope present in human B lymphocytes. This
antibody may be used to aid in the identification of normal and neoplastic cells of B-lymphocytic lineage.
References
1. Adams B, et al., Genes Dev. 6(9):1589-1607, 1992.
2. Cobaleda C, et al., Nat Immunol. 8(5):463-470, 2007.
3. Holmes ML, et al., Immunol Cell Biol. 86(1):47-53, 2008.
4. Feldman AL, et al., Adv Anat Path. 14(5):323-334, 2007.
5. Jensen KC, et al., Mod Pathol. 20(8):871-877, 2007.
6. Mhawech-Fauceglia P, et al., J Clin Pathol. 60(6):709-714, 2007.
158 NEXT>
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<PREV
NEXT>
PD-1 (also known as NAT)
(MRQ-22)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4448 05973783001
Mouse Monoclonal
Lymph Node, Tonsil
Cytoplasmic
50 tests
IgG1
Description
Programmed death-1 (PD-1) is a member of the CD28 family of receptors that includes CD28, cytotoxic T-lymphocyte-associated antigen
4 (CTLA-4), inducible costimulator (ICOS) and Band T-lymphocyte attenuator. These receptors play a role in the cellular immune response.
For example, CD28 serves as a costimulatory receptor that enhances T-cell activation, whereas CTLA-4 serves as an inhibitor of T-cell
activation. PD-1 also has an inhibitory function on T cells and B cells and is important in peripheral tolerance. There are at least 2 ligands
for PD-1: PD-L1 and PD-L2, which are expressed on a range of cells.
CD28 is constitutively expressed on most or all CD4+ T cells and approximately 50% of CD8+ T cells, whereas CTLA-4 is not expressed
on resting T cells. PD-1 is also expressed on activated T cells, B cells and myeloid cells. Iwai and coworkers studied the microanatomic
distribution of PD-1 in human tonsil and found that PD-1 is expressed on most T cells and a small subset of B cells in the light zone
of germinal centres, but not elsewhere in the tonsil. On that basis, it was postulated that PD-1 may play a role in the process of clonal
selection of centrocytes, which occurs in this subanatomic site in germinal centres.
PD-1 is a new marker of angioimmunoblastic lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and bcl-6,
PD-1 is expressed by few B cells, so it may be a more specific and useful diagnostic marker in angioimmunoblastic lymphoma. It also seems
to stain a greater percentage of CD3-positive neoplastic cells in angioimmunoblastic lymphoma than either CD10 or bcl-6. In addition, PD-1
expression provides new evidence that angioimmunoblastic lymphoma is a neoplasm derived from germinal centre-associated T cells.
PD-1 expression in angioimmunoblastic lymphoma lends further support to this model of T-cell oncogenesis, in which specific subtypes of
T cells may undergo neoplastic transformation and result in specific distinct histologic, immunophenotypic and clinical subtypes of T-cell
neoplasia.
References
1. Bolstad AI, et al., Arthritis Rheum. 48(1):174-85, 2003.
2. Dorfman DM, et al., Am J Surg Pathol. 30(7):802-10, 2006.
3. Hamanishi J, et al., Proc Natl Acad Sci U S A. 104(9):3360-5, 2007.
4. Iwai Y, et al., Immunol Lett. 83(3):215-20, 2002.
5. Kobayashi M, et al., J Rheumatol. 32(11):2156-63, 2005.
6. Konishi J, et al., Clin Cancer Res. 10(15):5094-100, 2004.
7. Mataki N, et al., Am J Gastroenterol. 102(2):302-12, 2007.
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Perforin
(MRQ-23)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4394 05463637001
Mouse Monoclonal
Tonsil, Lymph Node
Perinuclear Cytoplasmic
50 tests
IgG1
Lymphoma
Description
Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target
cells and activate apoptosis, the cell death program. The expression of perforin is upregulated in activated CD8+ T cells, but in NK cells the
expression is constitutively very high and stable. Perforin expression can also be stimulated in some activated CD4+ T cells. CD8+ T cells
and NK cells use both the perforin ⁄ granzyme and the Fas ⁄ FasL pathways, while CD4+ T cells preferentially use the Fas ⁄ FasL pathway.
Although some investigators report a cytolytic potential of CD4+ T cells, it appears more likely that CD8+ T cells are the major effector
population in Th1- associated inflammatory skin diseases. The role of perforin-mediated cytotoxicity has been demonstrated in various
auto-immune diseases. In vitro and in vivo studies suggest that the cytotoxicity of CTLs may be mediated by cytotoxic granules in certain
inflammatory diseases in humans. In addition, it seems that T-cell cytotoxicity against keratinocytes is mediated by perforin in some
inflammatory skin diseases.
Other authors suggest that perforin may have a dual role in alloimmune response (organ transplant applications). In one regard, it has a cytolytic
function in acute rejection and, in contrast, it may be responsible for downregulating both CD4- and CD8-mediated alloimmune response.
References
1. Chu PG, et al., Ann Diagn Pathol. 3(2):104-33, 1999. Review.
2. Bittmann I, et al., Virchows Arch. 445(4):375-81, 2004. Epub 2004
Jul 29.
PGP 9.5
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. d’Amore ES, et al., Pediatr Dev Pathol. 10(3):181-91, 2007.
760-4434 05973953001
Rabbit Polyclonal
Nerve Tissue, Bowel Wall (Interstitial Cells of Cajal)
Cytoplasmic
50 tests
IgG
Small bowel wall, peritonitis
Description
Protein gene product 9.5 (PGP 9.5), also known as ubiquitin carboxylterminal hydrolase-1 (UCH-L1), is a 27-kD protein originally isolated
from whole brain extracts (1). Although PGP 9.5 expression in normal tissues was originally felt to be strictly confined to neurons and
neuroendocrine cells (2), it has been subsequently documented in distal renal tubular epithelium, spermatogonia, Leydig cells, ovocytes,
melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells and dermal
fibroblasts. LK Campbell, et al. demonstrated immunostaining of a plethora of different mesenchymal neoplasms with this antibody.
References
1. Bassotti G, et al., J Clin Pathol. 58(9):973-7, 2005.
2. Campbell LK, et al., Mod Pathol. 16(10):963-9, 2003.
3. Kasprzak A, et al., Pol J Pathol. 58(1):23-33, 2007. Review.
4. Mahalingam M, et al., J Cutan Pathol. 28(6):282-6, 2001.
5. Mahalingam M, et al., J Cutan Pathol. 33(1):51-6, 2006.
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Phosphohistone-H3 (PHH3) NEW
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-4591 06433405001
Rabbit Polyclonal
Tonsil
Nuclear
50 tests
Breast infiltrating ductal carcinoma
Description
Phosphohistone-H3 Rabbit Polyclonal Primary Antibody is used as an aid in identification of mitotic activity in tumour cells and diagnosis of
tumour grade differentiation within the context of an antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a
qualified pathologist.
References
2. Gurley, LR. Et al. J Biol Chem. 268:18431-4, 1993.
3.Hendzel, MJ, et al. J Biol Chem. 273:24470-8, 1998.
4. Colman H. et al. Am J Surg Pathol. 30:657-64, 2006.
5. Nasr MR, et al. Am J Dermatopathol. 30:117-22, 2008.
6. Kim YJ, et al. Am J Clin Pathol 128:118-25, 2007.
Placental Alkaline Phosphatase (PLAP)
(NB10)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2664 05267757001
Mouse Monoclonal
Placenta
Cytoplasmic
50 tests
IgG
Placenta, cytotrophoblasts
Description
PLAP (NB10) Mouse Monoclonal Primary Antibody immunoreacts with germ cell tumours and can discriminate between these and other
neoplasms. Somatic neoplasms (e.g. breast, gastro-intestinal, prostatic and urinary cancers) may also immunoreact with antibodies to
PLAP. PLAP antibody positivity in conjunction with keratin negativity favours seminoma over carcinoma. Germ cell tumours are usually
keratin positive, but they regularly fail to stain with EMA antibody, whereas most carcinomas stain with EMA antibody. This antibody has
shown cross-reaction with human intestinal alkaline phosphatase.
References
1. Jacobsen GK, et al., Acta Path Microb Immuno Scand Sect A.
92:323-329, 1984.
2. Paiva J, et al., Am J Pathol. 111:156-165, 1984.
3. Burke AP, et al., Hum Path. 19:663-670, 1988.
4. Manivel JC, et al., Am J Surg Path. 11:21-29, 1987.
5. Wick MR, et al., Hum Path. 18:946-954, 1987.
PMS2 NEW
(EPR3947)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4531 06419216001
Rabbit Monoclonal
Colon
Nuclear
50 tests
IgG
Description
Microsatellite instability (MSI) is characterised by genome-wide alterations in short, repetitive DNA sequences. It is caused by defects
in the nucleotide mismatch repair (MMR) system. Biologically, defective MMR results in a general increase in the mutation rate and
the development of a mutator phenotype. In colorectal cancer (CRC), high-level MSI was first described in tumours from patients with
hereditary non-polyposis colorectal cancer (HNPCC). PMS2 (EPR3947) Rabbit Monoclonal Primary Antibody is a useful diagnostic and
prognostic marker for hereditary non-polyposis colorectal cancer (HNPCC).
References
1. Hampel H, et. al., N Engl J Med. 352:1851-60, 2005.
2. Gologan A, et. al., Clin Lab Med. 25:179-96, 2005.
3. Lagerstedt Robinson K, J Natl Cancer Inst. 99:291-9, 2007
4. Hendriks YM, et. al., Gastroenterology. 130:312-22, 2006.
5. Truninger K, et. al., Gastroenterology. 128:1160-71, 2005.
6. Warusavitarne J, et al., J Colorectal Dis. 22: 739-748, 2007.
7. Gill S, et. al., Clin Cancer Res. 11:6466, 2005.
Pneumocystis jiroveci (carinii)
(3F6)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2665 05267765001
Mouse Monoclonal
Infected Tissue
Membranous
50 tests
IgM/K
Description
Pneumocystis jiroveci (carinii) is a fungal organism which is detected in human tissues (typically in lung in immunocompromised patients)
in the trophozoite stage. Pneumocystis jiroveci (carinii) (3F6) Mouse Monoclonal Primary Antibody reacts with an epitope on the organism
which is resistant to formalin, picric acid, paraffin, as well as alcohol and xylene. No cross-reactivity has been demonstrated with other
fungi or parasitic organisms.
162 Primary Antibodies by alphabetical listing | IHC & ISH
Podoplanin
(D2-40)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4395 05463645001
Mouse Monoclonal
Lymph Node, Lymphangioma
Cytoplasmic
50 tests
IgG1
Tonsil
Description
Podoplanin is a transmembrane mucoprotein (38 kd) recognised by the D2-40 Mouse Monoclonal Primary Antibody. Podoplanin is
selectively expressed in lymphatic endothelium as well as lymphangiomas, Kaposi sarcomas and in a subset of angiosarcomas with
probable lymphatic differentiation. Podoplanin has also been shown to be expressed in epithelioid mesotheliomas, hemangioblastomas and
seminomas.
References
1. Ordonez N, Adv Anat Pathol. 13(2):83-8, 2006.
2. Ordonez N, Hum Pathol. 36(4):372-80, 2005.
3. Niakosari F, et al., Arch Dermatol. 141(4):440-4, 2005.
4. Galambos C, et al., Pediatr Dev Pathol. 8(2):191-9, 2005.
5. Fukunaga M, Histopathology. 46(4):396-402, 2005.
6. Chu AY, et al., Mod Pathol. 18(1):105-10, 2005.
7. Franke FE, et al., J Cutan Pathol. 31(5):362-7, 2004.
8. Fogt F, et al., Oncol Rep. 11(1):47-50, 2004.
9. Kahn HJ, et al., Mod Pathol. 15(4):434-40, 2002.
Progesterone Receptor (PR), CONFIRM
(1E2)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-2223 05277990001 (50 tests);
790-4296 05278392001 (250 tests)
Rabbit Monoclonal
Breast
Nuclear
50 tests (790-2223), 250 tests (790-4296)
IgG
Breast Ductal Carcinoma
Description
CONFIRM PR (1E2) Rabbit Monoclonal Primary Antibody is intended for laboratory use for the qualitative detection of progesterone receptor
(PR) antigen in sections of formalin-fixed, paraffin-embedded tissue on a VENTANA automated slide stainer with VENTANA detection kits
and ancillary reagents. CONFIRM PR (1E2) that recognises the A and B forms of human progesterone receptor and is indicated as an aid
in the management, prognosis and prediction of therapy outcome of breast cancer. One of the important markers in breast cancer today
is progesterone receptor (PR), due to its role in determining the functionality of estrogen receptors present in breast cancer cells. The
presence of estrogen receptor (ER), however, does not guarantee a response to endocrine therapy. Half of all patients with ER positive
primary tumours fail to respond. In tumour cells, genetic expression is often inaccurate, leading to variant protein expression. It is proposed
that mutant ER, that no longer bind estrogen or that no longer perform signal transduction, may be the cause of the failure to respond to
endocrine therapy. One way to evaluate the functionality of the ER present in a breast carcinoma is to determine if proteins regulated by ER
are expressed. Progesterone receptor is such a protein and has been used to monitor for functional ER for many years.
References
1. Bevitt DJ, Milton ID, et al., J. Pathol. 83(2):228-232, 1997.
2. Dickson RB, Lippman ME, et al., Kluwer Acad, Publ. 1992; 285-286.
3. Wei LL, et al., Cancer Res. 54(2):340-3, 1994.
4. Doglioni C, et al., Am. J. Pathol. 137(5):999-1005, 1990.
5. Greene GL, et al., Immun. Appr. Diag. and Ther. Breast Cancer. 119135, 1987.
6. Press MF, Greene GL, et al., Endocrinology. 122(3):1165-1175, 1988.
7. Winborn WB, Sheridan PJ, et al., Pancreas. 2(3):289-94, 1987.
8. Money SR, Muss W, et al., Surgery. 106(6):975-8, 1989.
9. Roche PC, et al., Mayo Clin. Proc. 69(1):57-58, 1994.
10. PDQ. http://icisun.nci.nih…t_cancer_Physician.html.
11. Horwitz KB, et al., J. Steroid Biochem. Mol. Biol. 49(4-6):295302, 1994.
12. Van Agthoven T, Timmermans M, et al., Int. J. Cancer. 63(6):790793, 1995.
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Prolactin
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
<PREV
760-2803 05268249001
Rabbit Polyclonal
Normal Pituitary
Cytoplasmic
50 tests
Pituitary
Description
Prolactin Rabbit Polyclonal Primary Antibody is a useful marker in classification of pituitary tumours and the study of pituitary disease. It
reacts with prolactin-producing cells. Such prolactin-producing cells can also be found in prostate epithelium.
References
1. Asa SL, et al., Arch Pathol Lab Med. 106:360, 1982.
2. Duello TM, et al., Amer J Anat. 158:463, 1980.
3. Minniti G, et al., Surg Neurol. 57(2):99-103, 2002.
4. Popadic A, et al., Surg Neurol. 51(1):47-54, 1999.
5. Nevalainen MT, et al., J Clin Invest. 99(4):618-27, 1997.
PSA (Prostate Specific Antigen), CONFIRM
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-2506 05266939001
Rabbit Polyclonal
Prostate
Cytoplasmic
50 tests
IgG
Prostate
Description
CONFIRM Prostate Specific Antigen (PSA) is directed against both free and bound human prostate specific antigen (PSA). PSA is a 33-34
kD chymotrypsin like serine protease (kallikrein family) specifically expressed by prostate epithelium. PSA is present in the cytoplasm
of benign and malignant prostate epithelium. It is useful for identification of adenocarcinoma of the prostate in metastatic sites and for
differentiating prostate adenocarcinoma from urothelial carcinoma.
References
1. Nadji M, et al., Cancer. 48(5):1229-1232, 1981.
2. Oesterling JE, J Urology. 145(5):907-923, 1991.
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PSAP (Prostatic Acid Phosphatase)
(PASE/4LJ)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4272 05269342001
Mouse Monoclonal
Prostate
Cytoplasmic
50 tests
IgG1
Prostate
Description
PSAP (PASE/4LJ) Mouse Monoclonal Primary Antibody reacts with prostatic acid phosphatase in the glandular epithelium of normal and
hyperplastic prostate, carcinoma of the prostate and metastatic cells of prostatic carcinoma. This marker may be helpful in pinpointing the
site of origin in cases of metastatic carcinoma of the prostate and is considered a more sensitive marker than PSA. However, it offers less
specificity. Nevertheless, PSAP complements PSA in the right clinical context.
References
1. Ansari MA, et al., Am J Clin Path. 76:94-98, 1981.
2. Nadji M, et al., Ann NY Acad Sci. 390:133-141, 1982.
3. Kimura N, et al., Virchows Arch A. 4:247-251, 1986.
4. Kidwai N, et al., Breast Cancer Res. 6(1):R18-23, 2004.
5. Kuroda N, et al., Pathol Int. 49(5):457-61, 1999.
6. Elgamal AA, et al., Urology. 44(1):84-90, 1994.
Renal Cell Carcinoma (RCC)
(PN-15)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4273 05269369001
Mouse Monoclonal
Kidney, Renal Cell Carcinoma
50 tests
IgG1/K
Kidney
Description
Renal Cell Carcinoma (PN-15) Mouse Monoclonal Primary Antibody recognises a 200 kD glycoprotein localised in the brush border of
the proximal renal tubule. This antibody immunoreacts with approximately 90% of primary renal cell carcinomas and approximately 85%
of metastatic renal cell carcinomas. Other tumours that may react with this antibody are parathyroid adenoma, an occasional breast
carcinoma. Nephroblastoma, oncocytoma, mesoblastic nephroma, transitional cell carcinoma and angiomyolipoma are not labelled with
this antibody.
References
1. Avery AK, et al., Am J Surg Pathol. 24(2):203-210, 2000.
2. McGregor DK, et al., Am J Surg Pathol. 25(12):1485-1492, 2001.
3. Gokden N, et al., Appl Immunohistochem Mol Morphol.
11(2):116-9, 2003.
4. Ordonez NG, et al., Human Pathology. 35(6):697-710, 2004.
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S100, CONFIRM
(4C4.9)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
790-2914 05278104001
Mouse Monoclonal
Melanoma
Cytoplasmic
50 tests
IgG2a
Melanoma
Description
S100 was first detected in nervous tissue and has subsequently been found in a number of other cell types including glial and neural cells,
Schwann cells, melanocytes and nevus cells, cells of cartilaginous and fatty tissue, myoepithelial cells and also Langerhans cells. S100 is
a sensitive marker for melanoma. Antibodies against S100 stain amelanotic melanomas more strongly than pigmented tumours and detect
melanomas that are often negative for other melanocytic markers, such as desmoplastic melanomas. Although S100 is highly sensitive for
melanomas, many non-melanocytic tumours also show S100 positivity. S100 typically is present in neural cells (glial cells and Schwann
cells) and their corresponding tumours.
References
1. Taylor RT, et al., W.B. Saunders Co, Philadelphia, 1994.
S100, CONFIRM
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
2. Kahn HJ, et al., Amer. J. Clin. Pathol. 1983; 79(3): 341-347.
760-2523 05267072001
Rabbit Polyclonal
Melanoma
Cytoplasmic
50 tests
Melanoma
Description
CONFIRM S100 Rabbit Polyclonal Primary Antibody contains rabbit antiserum directed against an epitope found on S100 protein and may
be used to aid in the identification of cells of normal and abnormal neuronal and neuroendocrine lineage and as an aid in the diagnosis of
anaplastic tumours.
S100 is a sensitive marker for melanoma. It stains amelanotic melanomas more strongly than pigmented tumours and detects melanomas
that are often negative for other melanocytic markers such as desmoplastic melanomas. The staining is cytoplasmic, the proportion of
positive cells varying from 10 to 70 percent or more.
Although S100 is highly sensitive for melanomas, many non-melanocytic tumours also show S100 positivity. S100 typically is present in
neural cells (glial cells and Schwann cells) and their corresponding tumours.1 An advantage of the ubiquity of strongly immunoreactive
S100 protein in peripheral nerves, which are present in almost any section of normal or diseased tissue, is that it provides a built in positive
control for most immunostains for S100.
References
1. Taylor RT, et al., WB Saunders Co, Philadelphia, 1994.
2. Kahn HJ, et al., Amer J Clin Pathol. 79(3):341-347, 1983.
3. True L. Atlas of Diagnostic Immunohistopathology. 1990.
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Smoothelin NEW
(R4A)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4592 06433278001
Mouse Monoclonal
Bladder, Leiomyosarcoma
Cytoplasmic (MP), Nuclear
50 tests
IgG1
Urinary transitional cell carcinoma
Description
Smoothelin (R4A) Mouse Monoclonal Primary Antibody is used as an aid in the distinction of terminally differentiated smooth muscle
cells, smooth muscle neoplasms of the gastro-intestinal tract and other organs and staging of bladder carcinoma within the context of an
antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
References
1. Kramer, J et al. J Mol Med. 77:294-8, 1999.
2. van der Loop, FT et al. J Cell Biol. 134:401-411, 1996.
3. Maake, C et al. J Urol. 175:1152-1157, 2006.
4. Jimenez, RE et al. Adv Anat Pathol. 7:13-25, 2000.
5. Kuijpers, et al. Eur Urol. 52:1213-21, 2007.
Somatostatin
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
6. Paner, GP et al. Am J Surg Pathol. 33:91-8, 2009.
7. Paner, GP et al. Am J Surg Pathol. 34:792-9, 2010.
8. Council, L et al. Mod Pathol. 22:639-650, 2009.
9. Coco, DP et al. Am J Surg Pathol. 33(12):1795-801, 2009.
760-2667 05267781001
Rabbit Polyclonal
Pancreas
Cytoplasmic
50 tests
Pancreas
Description
Somatostatin Rabbit Polyclonal Primary Antibody is a useful marker of D cells of pancreatic islet cells. D-cell identification can be used
to identify hyperplasia of the pancreatic islets as well as islet cell tumours such as somatostatinomas. Somatostatin can also be found in
gastro-intestinal and broncho-pulmonary endocrine cells, thymic endocrine cells and thyroid C cells. Somatostatin suppresses gastric acid
secretion, gall bladder contractions and pancreatic enzyme secretion.
References
1. Krejs GJ, et al., N. Eng J Med. 301:285-292, 1979.
2. Somers G, et al., Gastroenterology. 85:1192-1198, 1983.
3. Erlandsen SL, et al., Williams and Wilkins, Baltimore. 140-155, 1980.
5. Tzaneva MA, et al., Acta Histochem. 105(2):191-201, 2003.
6. Quartu M, et al., J Chem Neuroanat. 6(2):79-99, 1993.
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Spectrin
(RBC2/3D5)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4274 05269377001
Mouse Monoclonal
Bone Marrow
Membranous
50 tests
IgG2b/K
Bone Marrow, red blood cell precursors
Description
Spectrin is a cytoskeletal protein which is found in muscles, red blood cells and red blood cell precursors. Spectrin (RBC2/3D5) Mouse
Monoclonal Primary Antibody is useful in the diagnosis of erythroid leukaemias.
References
4. Terada N, et al., J Anat. 190(3):397-404, 1997.
1. Sadahira Y, et al., J Clin Pathol. 52(12):919-21, 1999.
2. Nehls V, et al., Am J Pathol. 142(5):1565-73, 1993.
3. Muller M, et al., J Vet Med A Physiol Pathol Clin Med. 48(1):51-7, 2001
SV40 (Simian Virus 40)
(MRQ-4)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4449 05973775001
Mouse Monoclonal
SV80 or SV-T2 cells, SV40 infected tissue
Nuclear
50 tests
IgG1
Brain
Description
SV40, Simian Virus 40 is a polyomavirus that is found in both monkeys and humans. Like other polyomaviruses, SV40 is a DNA virus that
has the potential to cause tumours. SV40 is believed to suppress the transcriptional properties of tumour-suppressing p53 in humans
through the SV40 large T-antigen and SV40 small T-antigen. It is generally assumed that large T-antigen is the major protein involved in
neoplastic processes and the large T-antigen predominantly exerts its effect through deregulation of tumour suppressor p53, which is
responsible for initiating regulated cell death (apoptosis) or cell cycle arrest when a cell is damaged. A mutated p53 gene may contribute
to uncontrolled cellular proliferation, leading to a tumour. The hypothesis that SV40 might cause cancer in human has been a particularly
controversial area of research. Some research has suggested that SV40 is associated with brain tumours, bone cancers, non-Hodgkin’s
lymphoma and malignant mesothelioma. SV40 may act as a cocarcinogen with crocidolite to cause mesothelioma.
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Synaptophysin NEW
(MRQ-40)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4595 06433324001
Rabbit Monoclonal
Pancreatic Islet Cells
Cytoplasmic
50 tests
IgG1
Lung small cell carcinoma
Description
Synaptophysin (MRQ-40) Rabbit Monoclonal Primary Antibody is used as an aid in the identification of neuroendocrine neoplasms within
the context of an antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
References
1. Navone, F et al. J Cell Biol 103:2511-2527, 1986.
2. Wiedenmann, B et al. Cell. 41:1017-1028, 1985.
3. Kayser, K et al. Path Res Pract. 183:412-417, 1988.
4. Son, EI et al. Pathol Int. 53(2):67-73, 2003.
5. Conner, MG et al. Ann Diagn Pathol. 6(6):345-8, 2002.
T-bet NEW
(MRQ-46)
Part Number
Type
Control
Localisation
Quantity
Isotype
6. Lyda, MH et al. Hum Pathol. 31(8):980-7, 2000.
7. Skacel, M et al. Appl Immunohistochem Mol Morphol. 8(3):302-9, 2000.
8. Morrison, CD et al. Semin Diagn Pathol. 17(3):204-15, 2000.
9. Kamisawa, T et al. Pathol Res Pract. 192(9):901-8, 1996.
760-4598 06433391001
Rabbit Monoclonal
Tonsil, Hairy Cell Leukaemia
Nuclear
50 tests
IgG1
Hairy cell leukaemia
Description
T-bet is used as an aid in the differentiation of hairy cell leukaemia, Hodgkin’s disease and precursor B-cell lymphoblastic leukaemia/
lymphoblastic lymphoma within the context of an antibody panel, the patient’s clinical history and other diagnostic tests evaluated by a
qualified pathologist.
References
1. Szabo, SJ et al. Cell. 100(6):665-69, 2000.
2. Zhang, WX et al. Genomics. 70(1):41-8, 2001.
3. Johrens, K et al. Am J Surg Pathol. 31(8):1181-1185, 2007.
4. Atayar, C et al. Am J Pathol. 166:127-134, 2005.
5. Dorfman, DM et al. Am J Clin Pathol. 122:292-297, 2004.
6. Harashima, A et al. Leuk Res. 29(7):841-8, 2005.
7. Marafioti, T et al. Am J Pathol. 162:861-871, 2003.
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TAG-72
(B72.3)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-2669 05267803001
Mouse Monoclonal
Breast Carcinoma
Cytoplasmic
50 tests
IgG1/K
Breast adenocarcinoma
Description
TAG-72 (B72.3) Mouse Monoclonal Primary Antibody recognises a high molecular weight glycoprotein called TAG-72 that is present
in human adenocarcinomas and in lesser amounts, non-neoplastic tissues. It has also been found to be useful to distinguish between
mesothelioma and adenocarcinoma, however, false positive reactions can occur so results must be interpreted with the utmost caution.
References
1. Thor A, et al., Cancer Res. 46:3118, 1986.
2. Schlom J, et al., Tumourmarker Oncology. 2:3, 1987.
3. Johnston WW, et al., Hum Pathol. 17:501-513, 1986.
4. Lundy J, et al., Ann Aurg. 203:399-402, 1986.
5. Kline TS, et al., Cancer. 63:2253-2256, 1989.
6. Chhieng DC, et al., Hum Pathol. 34(10):1016-21, 2003,
7. Ordonez NG, et al., Am J Surg Pathol. 22(10):1203-14, 1998.
TdT (Terminal Deoxynucleotidyl Transferase)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2670 05267811001
Rabbit Polyclonal
TdT Positive Lymphomas, Thymus
Nuclear
50 tests
Thymus, cortical thymocytes
Description
Terminal Deoxynucleotidyl Transferase (TdT) Rabbit Polyclonal Primary Antibody labels normal cortical thymocytes and primitive
lymphocytes. TdT antibody detects an enzyme found in the nucleus of normal hematopoietic cells, normal cortical thymocytes and in
the cytoplasm of megakaryocytes of the bone marrow. TdT expression is seen in over 90% of acute lymphoblastic lymphoma/leukaemia
cases with the exception of pre-B-Cell ALL. TdT expression is not seen in normal mature T or B lymphocytes. TdT antibody is positive for
approximately one third of all cases of chronic myeloid leukaemia, making it a good indicator of better response to chemotherapy.
References
1. Elias JM, et al., TdT. A Practical Approach to Diagnosis, ASCP Press, 3. Orazi A, et al., Mod Pathol. 7(5):582-6, 1994.
4. Suzumiya J, et al., J Pathol. 182(1):86-91, 1997.
Chicago 1990; 312-316.
2. Arber DA, et al., Am.J Clin Pathol. 106(4):462-8, 1996.
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Thymidine Phosphorylase, CONFIRM
(P-GF.44C)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4454 05976502001
Mouse Monoclonal
Astrocytes in Normal Brain, Lymphocytes in Spleen
50 tests
IgG1
Breast carcinoma
Description
CONFIRM Thymidine Phosphorylase (P-GF.44C) Mouse Monoclonal Primary Antibody recognises human thymidine phosphorylase, an
angiogenic factor, expressed in varying levels by macrophages, stromal cells, glial cells and some epithelial cells. Thymidine phosphorylase
shows a cytoplasmic and/or nuclear location. This antibody exhibits a nuclear and/or cytoplasmic staining pattern and may be used to
aid in the detection of thymidine phosphorylase in carcinoma of the breast, oesophagus, stomach, colon and rectum, pancreas, head and
neck, ovaries and lung.
References
1. Fox SB, et al., Biochemistry. 20:3615-3621, 1981.
2. Haraguchi M, et al., Biochem Pharmacol. 74:1555-1567, 2007.
3. Desgranges C, et al., Biochem Biophys Acta. 654:211-218, 1981.
Thyroglobulin
(2H11+6E1)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Yoshimura A, et al., Biochem Biophys Acta. 1034:107-113. 1990.
5. De Bruin M, et al., Cancer Ther. 4:99-124, 2006.
760-2671 05267820001
Mouse Monoclonal
Thyroid
Cytoplasmic
50 tests
IgG1
Follicular carcinoma of thyroid
Description
Thyroglobulin (2H11+6E1) Mouse Monoclonal Primary Antibody reacts with human thyroglobulin as demonstrated by a single band
of immunoblotting in a lysate of human thyroid tissue. The vast majority of follicular carcinomas of the thyroid will give positive
immunoreactivity for thyroglobulin even though sometimes only focal. Poorly differentiated carcinomas of the thyroid are frequently
thyroglobulin negative. Adenocarcinomas of other than thyroid origin do not react with this antibody.
References
1. Bellet D, et al., J Clin Endocrin Metab. 56:530-533, 1983.
2. Heffess CS, et al., Cancer. 95(9):1869-78, 2002.
3. Bejarano PA, et al., Appl Immunohistochem Mol Morphol. 8(3):
189-94, 2000.
4. Judkins AR, et al., Hum Pathol. 30(11):1373-6, 1999.
5. Hammer SP, et al., Hum Pathol. 29(12):1393-402, 1998.
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Thyroid Stimulating Hormone (TSH)
(polyclonal)
Part Number
Type
Control
Localisation
Quantity
760-2709 05268184001
Rabbit Polyclonal
Normal Pituitary
Cytoplasmic
50 tests
Pituitary
Description
Thyroid Stimulating Hormone (TSH) Rabbit Polyclonal Primary Antibody is a useful marker in classification of pituitary tumours and the
study of pituitary disease. It reacts with TSH-producing cells (thyrotrophs). TSH is a pituitary hormone of 28 kD, which stimulates thyroid
growth and production of thyroid hormones.
References
1. Batanero E, et al., Brain Behav Immun. 6(3):249-64, 1992.
2. Kovalic JJ, et al., J Neurooncol. 16(3):227-32, 1993.
3. Gessl A, et al., J Clin Endocrinol Metab. 79(4):1128-34, 1994.
4. Sanno N, et al., J Clin Endocrinol Metab. 80(8):2518-22, 1995.
Thyroid Transcription Factor-1 (TTF-1), CONFIRM
(8G7G3/1)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4398 05479312001
Mouse Monoclonal
Lung Carcinoma, Lung, Thyroid
Nuclear
50 tests
IgG1
Description
TTF-1 is a 38 kD nuclear protein member of the family of homeodomain transcription factors. Exclusive expression in epithelial cells of the
thyroid gland and lung allows TTF-1 to be a useful antibody for the classification of tumours arising in these organs. In context with other
data, TTF-1 becomes useful in the investigation of metastatic adenocarcinomas of an unknown origin. TTF-1 demonstrates an intranuclear
staining pattern and normal thyroid gland follicle cells and lung parenchymal alveolar lining cells serve as excellent tissue internal controls.
References
1. Dongfeng T, et al., Human Pathology. 34:597-604, 2003.
2. Ordonez N, American J of Surgical Pathol. 25:363-372, 2001.
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Topoisomerase IIa, CONFIRM
(JS5B4)
Part Number
Type
Control
Localisation
Quantity
790-4371 05479339001
Rabbit Monoclonal
Tonsil, Spleen
Nuclear
50 tests
Breast carcinoma
Description
CONFIRM Topoisomerase IIα (JS5B4) Rabbit Monoclonal Primary Antibody is designed to qualitatively detect the presence of
topoisomerase IIα expressing cells. During the cell cycle, topoisomerase IIα is expressed in the G1, S, G2 and M phase but is absent in the
G0 (quiescent) phase. Assessment of the proportion of positive staining cells for topoisomerase IIα may aid in evaluation of the proliferative
activity of normal and/or neoplastic tissue.
References
1. Kreipe H, et al., AM J Pathol. 142:3-9, 1993
2. Rudolph P, et al., AM J Pathol. 147:1615-25, 1995.
3. Kreipe H, et al., AM J Pathol. 142:651-657, 1993.
4. Kellner U, et al., J of Histochem and Cytochem. 45:251-264, 1997.
TRAcP (Tartrate-Resistant Acid Phosphatase)
(9C5)
Part Number
Type
Control
Localisation
Quantity
Isotype
760-4275 05269385001
Mouse Monoclonal
Hairy Cell Leukaemia
Cytoplasmic
50 tests
IgG2b
Bone marrow
Description
The histochemical identification of hairy cell leukaemia via tartrate-resistant acid phosphatase assay has been a standard for over two
decades. TRAcP (9C5) Mouse Monoclonal Primary Antibody labels the cells of hairy cell leukaemia (HCL) with a high degree of sensitivity
and specificity. Other cells stained with this antibody are tissue macrophages and osteoclasts, which also express abundant TRAcP
activity.
References
1. Janckila AJ, et al., Blood. 85(10):2839-44, 1995.
2. Yaziji H, et al., Am. J Clin Pathol. 104(4):397-402, 1995.
3. Janckila AJ, et al., J Histochem Cytochem. 44(3):235-44, 1996.
4. Janckila AJ, et al., Hybridoma. 16(2):175-82, 1997.
5. Hoyer JD, et al., Am J Clin Pathol. 108(3):308-15, 1997.
6. Janckila AJ, et al., Biotech Histochem. 73(6):316-24, 1998.
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Tryptase
(G3)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
760-4276 05269393001
Mouse Monoclonal
Mast Cell-Containing Tissue
Cytoplasmic
50 tests
IgG1
Cervix
Description
Tryptases constitute a subfamily of trypsin-like proteinases, stored in the mast cell secretory granules and basophils. Upon cellular
activation, these enzymes are released into the extracellular environment. Tryptase (G3) Mouse Monoclonal Primary Antibody is a good
marker for mast cells, basophils and their derivatives.
References
1. Fiorucci L, et al., Cell Mol Life Sci. 61(11):1278-95, 2004.
2. Li CY, et al., Leuk Res. 25(7):537-41, 2001.
3. Jordan JH, et al., Hum Pathol. 32(5):545-52, 2001.
4. Gordon LK, et al., Clin Immunol. 94(1):42-50, 2000.
5. Aoki M, et al., Int Arch Allergy Immunol. 130(3):216-23, 2003.
Tyrosinase, CONFIRM
(T311)
Part Number
Type
Control
Localisation
Quantity
Isotype
790-4365 05479347001
Mouse Monoclonal
Melanoma
Cytoplasmic
50 tests
IgG2a
Melanoma, with area showing normal melanoctyes
Description
Tyrosinase is an enzyme involved in the production of melanin pigments. This enzyme catalyses the formation of melanin within the cells
and thus becomes a useful marker for the presence of melanocytes and melanosomes. As a marker of melanocytic lineage, tyrosinase is
localised to melanocytes which can be found on the dermal/epidermal junction of normal skin, but is not detected in other normal cells.
Expression of tyrosinase is also found in melanocytic lesions including benign nevi and the majority of primary and metastatic malignant
melanomas and not in non-melanocytic tumour types.
References
1. Chen Y, et al., Proc Natl Acad Sci USA. 92:8125-8129, 1995.
2. Jungbluth A, et al., Pathol Res Pract. 196:235-242, 2000.
3. Clarkson K, et al., J Clin Pathol. 54:196-200, 2001.
4. Kwon B, J of Invest Dermatol. 100:134S-140S, 1993.
5. Hofbauer G, et al., J Cutan Pathol. 25:204-209, 1998.
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Uroplakin III NEW
(SP73)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4533 06419232001
Rabbit Monoclonal
Bladder
50 tests
IgG1
Description
Uroplakins (UPs) are a family of transmembrane proteins (UPs Ia, Ib, II and III) that are specific differentiation products of urothelial cells.
In non-neoplastic mammalian urothelium, UPs are expressed in the luminal surface plasmalemma of superficial (umbrella) cells, forming
complexes of 16-nm crystalline particles. Moll, et al. reported that UPIII was detectable immunohistochemically in 29 of 55 primary (53%)
and 23 of 35 metastatic (66%) urothelial carcinomas, whereas many non-urothelial carcinomas were UPIII-negative. The authors concluded
that UPIII should be a valuable marker, especially for the specific identification of urothelial carcinomas in patients with metastases of
unknown primary site.
References
1. Angel CE, et al., Blood. 113:1257-67, 2009.
2. Moll R et al., Am J Pathol. 147:1383-1397, 1995.
Villin
(CWWB1)
Part Number
Type
Control
Localisation
Quantity
Isotype
3. Olsburgh J, et al., J Pathol. 199: 41-49, 2003.
4. Ohtsuka Y, et. al., BJU Int. 97:1322-6, 2006.
760-4277 05269407001
Mouse Monoclonal
Small Bowel Mucosa, Colonic Mucosa
50 tests
IgG1
Colon
Description
Villin is a 95kD glycoprotein of microvilli associated with rootlet formation in gastro-intestinal mucosal epithelium. Villin (CWWB1) Mouse
Monoclonal Primary Antibody labels the brush border area in the gastro-intestinal mucosal epithelium. This antibody has been useful in
differentiating gastro-intestinal adenocarcinoma, neuroendocrine carcinomas and ovarian adenocarcinomas from adenocarcinomas from
other organs. Also labelled by this antibody are Merkel cells of the skin.
References
1. Werling RW, et al., Am J Surg Path. 27(3):303-310, 2003.
2. Tan J, et al., Hum Pathol. 29:390-396, 1998.
3. Goldstein NS, et al., Am J Clin Pathol. 116:319-325, 2001.
4. Lau SK, et al., Hum Pathol. 33:1175-1181, 2002.
5. Tamboli P, et al., Arch Pathol Lab Med. 126:1057-1063, 2002.
6. Zhang PJ, et al., Arch Pathol Lab Med. 123:812-816, 1999.
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Vimentin, CONFIRM
(V9)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
790-2917 05278139001
Mouse Monoclonal
Lymph Node, Skin
Cytoplasmic
50 tests
IgG/K
Tonsil
Description
Vimentin antigen is expressed in endothelial cells, fibroblasts, smooth muscle cells and lymphoid cells. A number of tumours coexpress vimentin and cytokeratin, for example thyroid carcinomas, pleomorphic adenomas of the salivary glands, ovarian carcinomas
and some renal carcinomas. Co-expression of desmin and vimentin has been reported in a number of soft tissue tumours, for example
rhabdomyosarcomas, leiomyosarcomas and alveolar soft tissue sarcomas.
References
1. Sheibani K, et al., Am J Surg Pathol. 12(1): 28-34, 1988.
2. Azumi N, et al., Am J Clin Pathol. 88(3): 286-296, 1987.
3. Benjamin E, et al., J Pathol. 152(4): 253-263, 1987.
Vimentin, CONFIRM
(Vim 3B4)
Part Number
Type
Control
Localisation
Quantity
Isotype
4. Miettinen M, et al., Arch Dermatol. 121(6): 736-741, 1985.
5. Osborn M, et al., Eur J Cell Biol. 34(1): 137-143, 1984.
760-2512 05266998001
Mouse Monoclonal
Intestine, Liver
Cytoplasmic
50 tests
IgG2a
Tonsil
Description
Vimentin is the major and usually only intermediate filament in a variety of non-muscle mesenchymal cells, including all fibroblastic cells,
endothelial cells, lipocytes, Schwann cells and macrophages. The pattern of expression of intermediate filaments may be used as a device
to aid in the classification of undifferentiated neoplasms. Vimentin is expressed in most malignant melanomas, either in the primary site or
in metastatic foci.
References
1. Weins B, Progen Biotechnik GmbH, Heidelberg, Germany, 1994.
2. Azumi N, et al., Am J Clin Pathol. 88(3): 286 296, 1987.
3. True LD, J.B. Lippincott Company, Philadelphia. 1990.
4. Taylor RT, et al., WB Saunders Company, Philadelphia. 1994.
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WT1
(6F-H2)
Part Number
Type
Control
Localisation
Quantity
Isotype
<PREV
NEXT>
760-4397 05435706001
Mouse Monoclonal
Malignant Mesothelioma, Kidney, Testicle
Nuclear
50 tests
IgG1/K
Mesothelioma
Description
WT1 is a suppressor gene located on chromosome 11p13. Wilms’ Tumour Protein (WT1) has been identified in proliferative mesothelial
cells, malignant mesothelioma, ovarian cystadenocarcinoma, gonadoblastoma, nephroblastoma and desmoplastic small round cell tumour.
Lung adenocarcinomas rarely stain positive with this antibody.
References
1. Ordonez NG, Am J Surg Pathol. 24(4):598-606, 2000.
2. Ordonez NG, Am J Surg Pathol. 22(11):1314-1327, 1998.
3. Charles AK, et al., Histopathology. 30(4):312-4, 1997.
4. Hussong J, et al., Mod Pathol. 10(11):1101-5, 1997.
ZAP-70
(2F3.2)
Part Number
Type
Control
Localisation
Quantity
Isotype
5. Barnoud R, et al., Am J Surg Pathol. 24(6):830-6, 2000.
6. Lucas DR, et al., Histopathology. 42(3):270-9, 2003.
7. Jimenez RE, et al., Am J Surg Pathol. 26(3):320-7, 2002.
8. Roberts F, et al., J Clin Pathol. 54(10):766-70, 2001.
760-4278 05269415001
Mouse Monoclonal
Chronic Lymphocytic Leukaemia, Tonsil, Lymph Node
Cytoplasmic
50 tests
IgG2a
Chronic lymphocytic leukaemia
Description
ZAP-70 is a 70 kD protein tyrosine kinase found in T cells and natural killer cells. Control of this protein translation is via the IgVH gene.
ZAP-70 protein is expressed in leukaemic cells of approximately 25% of Chronic Lymphocytic Leukaemia (CLL) cases as well. ZAP-70
expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70
positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig-unmutated CLL cases have
a poorer prognosis.
References
1. Wiestner A, et al., Blood. 101(12):4944-4951, 2003.
2. Crespo M, et al., N Eng J Med. 348(18):1764-1775, 2003.
3. Chen L, et al., Blood. 100(13):4609-14, 2002.
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We have partnered with Cell Marque Corporation to provide you with an extended menu of primary antibodies for
immunohistochemistry. The table below identifies all of the antibodies that are manufactured by Cell Marque.
α-1-Antichymotrypsin (ACT)
α-1-Antitrypsin (AAT)
α-Fetoprotein (AFP)
Actin, Muscle Specific
Actin, Smooth Muscle
Adrenocorticotrophin (ACTH)
Annexin A1
BCA-225
bcl-6
Beta-catenin
BG8, Lewisy
BOB.1
C3d
C4d
CA 125
CA 19-9
Calcitonin
Caldesmon
Calponin-1
CD1a
CD2
CD14
CD21 (2G9)
CD21 (EP3093)
CD25
CD31 (JC-70)
CD31 (1A10)
CD34
CD45
CD45R
CD56 (MRQ-42)
CD57
CD61
CD63
CD71
CD74
CD-138/syndecan-1
CD163
CDX-2
CEA (CEA31)
Collagen Type IV
COX-2 (Cyclooxygenase 2)
Cytokeratin (34bH11)
Cytokeratin 8 & 18
Cytokeratin 14
Cytokeratin 19
DOG1 (SP31)
E-Cadherin (EP700Y)
Ep-CAM (Epithelial Specific Antigen)
Factor VIII Related Antigen
Factor XIIIa (AC-1A1)
Factor XIIIa (EP3372)
Fascin
FSH (Follicle Stimulating Hormone)
Galectin-3
Gastrin
GCDFP-15
GFAP (Glial Fibrillary Acidic Protein)
Glucagon
GLUT-1
Glycophorin A
Glypican-3
Granzyme B
GH (Growth Hormone)
Herpes Simplex Virus I
Herpes Simplex Virus II
HGAL
HHV-8 (Human Herpes Virus Type 8)
HSA (Hepatocyte Specific Antigen)
Human Chorionic Gonadotropin (hCG)
Human Placental Lactogen (hPL)
IgA
IgD
IgG
IgM
Inhibin, alpha
Insulin
Ksp-Cadherin
Luteinizing Hormone (LH)
Lysozyme
Macrophage
Mammaglobin
Melanoma Associated Antigen
(KBA.62)
Melanoma Associated Antigen (PNL2)
Mesothelial Cell
MLH1 (M1)
MSH2
MUC2
MUC5AC
MUC6
MUM1
Myeloperoxidase
Myogenin
Myoglobin
Myosin, Smooth Muscle
Napsin A
Neurofilament
NGFR (Nerve Growth Factor Receptor)
NSE (Neuron Specific Enolase)
Oct-2
Oct-4
p21WAF1
p27kip1
Parathyroid Hormone (PTH)
PD-1
178 Perforin
PGP 9.5
Phosphohistone-H3 (PHH3)
Placental Alkaline Phosphotase (PLAP)
PMS2
Pneumocistis jiroveci (carinii)
Podoplanin
Prolactin
PSAP (Prostatic Acid Phosphatase)
Renal Cell Carcinoma (RCC)
Smoothelin
Somatostatin
Spectrin
SV40
T-bet
TAG-72
TdT (Terminal Deoxynucleotidyl
Transferase)
Thyroglobulin
Thyroid Stimulating Hormone (TSH)
TRAcP (Tartrate-Resistant Acid
Phosphatase)
Tryptase
Uroplakin III
Villin
WT1
ZAP-70
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Albumin, FITC
Part Number
Type
Control
Species
Quantity
760-2699 05268087001
Polyclonal
Frozen, Diseased Skin or Diseased Renal Tissue
Goat
50 tests
Renal tubule basement membrane
Description
FITC anti-Albumin Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed against human
albumin. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of human albumin in target tissue
(e.g. in the diagnosis of renal or dermal pathologies). FITC anti-Albumin is intended for laboratory use to qualitatively stain sections of
frozen tissue on a VENTANA automated slide stainer.
C1q, FITC
Part Number
Type
Control
Species
Quantity
760-2688 05267994001
Polyclonal
Goat
50 tests
Lupus Nephritis
Photo courtesy of Lois J. Arend, Ph.D., M.D., Dept. of Pathology and
Laboratory Medicine, University of Cincinnati Medical Ctr.
Description
FITC anti-C1q (complement 1q) Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed
against human C1q. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of complement 1q in
C1q target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-C1q is intended for laboratory use to qualitatively stain
sections of frozen tissue on a VENTANA automated slide stainer.
IHC & ISH | Immunofluorescence Antibodies
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C3, FITC
Part Number
Type
Control
Species
Quantity
<PREV
760-2686 05267978001
Polyclonal
Frozen, Kidney or Skin from Lupus Cases Containing C3 Deposits
Goat
50 tests
Kidney lupus
Description
FITC anti-C3 (complement 3) Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed
against human C3. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of complement 3 in C3
target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-C3 is intended for laboratory use to qualitatively stain sections
of frozen tissue on a VENTANA automated slide stainer.
C4, FITC
Part Number
Type
Control
Species
Quantity
760-2687 05267986001
Polyclonal
Sheep
50 tests
Glomerulus
Description
FITC anti-C4 (complement 4) Primary Antibody is a sheep-derived polyclonal antibody labelled with fluorescein and specifically directed
against human C4. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of complement 4 in C4
target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-C4 is intended for laboratory use to qualitatively stain sections
of frozen tissue on a VENTANA automated slide stainer.
Fibrinogen, FITC
Part Number
Type
Control
Species
Quantity
760-2685 05267960001
Polyclonal
Frozen, Placenta or Tissue Containing Clotted Blood
Goat
50 tests
Kidney , normal
Description
FITC anti-Fibrinogen Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed against
human fibrinogen. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of fibrinogen in target
tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-Fibrinogen is intended for laboratory use to qualitatively stain
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IgA, FITC
Part Number
Type
Control
Species
Quantity
| IHC & ISH
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760-2681 05267927001
Polyclonal
Frozen, Tonsil or Lymph Node
Goat
50 tests
IgA Nephropathy
Description
FITC anti-IgA (immunoglobulin A) Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed
against human IgA. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of immunoglobulin A
in IgA target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-IgA is intended for laboratory use to qualitatively stain
IgG, FITC
Part Number
Type
Control
Species
Quantity
760-2680 05267919001
Polyclonal
Frozen, Tonsil or Lymph Node Clotted Blood
Goat
50 tests
Membraneous Glomerulopthy
Description
FITC anti-IgG (immunoglobulin G) Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed
against human IgG. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of immunoglobulin G
in IgG target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-IgG is intended for laboratory use to qualitatively stain
IgM, FITC
Part Number
Type
Control
Species
Quantity
760-2682 05267935001
Polyclonal
Goat
50 tests
Plasma cells
Description
FITC anti-IgM (immunoglobulin M) Primary Antibody is a goat-derived polyclonal antibody labelled with flurescein and specifically directed
against human IgM. This reagent should be used in conjuction with a panel of antibodies to aid in the identification of immunoglobulin M
in IgM target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-IgM is intended for laboratory use to qualitatively stain
IHC & ISH | Immunofluorescence Antibodies
HOME
Kappa, FITC
Part Number
Type
Control
Species
Quantity
<PREV
760-2683 05267943001
Polyclonal
Frozen, Tonsil or Lymph Node Clotted Blood
Goat
50 tests
Description
FITC anti-Kappa Primary Antibody is a goat-derived polyclonal antibody labelled with fluorescein and specifically directed against human
kappa immunoglobulin light chains. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of
kappa in target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-Kappa is intended for laboratory use to qualitatively
stain sections of frozen tissue on a VENTANA automated slide stainer.
Lambda, FITC
Part Number
Type
Control
Species
Quantity
760-2684 05267951001
Polyclonal
Burro
50 tests
Description
FITC anti-Lambda Primary Antibody is a burro-derived polyclonal antibody labelled with fluorescein and specifically directed against
human lambda light chains. This reagent should be used in conjunction with a panel of antibodies to aid in the identification of lambda light
chains in target tissue (e.g. in the diagnosis of renal or dermal pathologies). FITC anti-Lambda is intended for laboratory use to qualitatively
stain sections of frozen tissue on a VENTANA automated slide stainer.
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Research Antibodies
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Research Antibodies
Hepatitis B Virus Core Antigen
(Polyclonal)
Part Number
Type
Status
Quantity
760-2646 05267579001
Rabbit Polyclonal
RUO
50 tests
Description
Hepatitis B Core Antigen Rabbit Polyclonal Primary Antibody labels cell nuclei infected with Hepatitis B virus, a common cause of Hepatitis
leading to cirrhosis. Hepatitis B is the second most common cause of parenterally transmitted Hepatitis.
Hepatitis B Virus Surface Antigen
(S1-210)
Part Number
Type
Status
Quantity
760-2647 05267587001
Mouse Monoclonal
RUO
50 tests
Description
Hepatitis B Surface Antigen Mouse Monoclonal Primary Antibody labels the cell cytoplasm infected with Hepatitis B virus, a common
cause of Hepatitis leading to cirrhosis. Hepatitis B is the second most common cause of parenterally transmitted Hepatitis.
Ventana part number in black, Roche item number in blue. The products that appear on this page are RUO (for research use only).
IHC & ISH | Research Antibodies
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Prostate Specific Antigen (PSA)
(ER-PR8)
Part Number
Type
Status
Quantity
760-4271 05269334001
Mouse Monoclonal
RUO
50 tests
Mesothelioma
Description
PSA is an antigen present in prostatic tissue and in the majority of prostatic carcinomas. This antibody recognises primary and metastatic
prostatic neoplasms and rarely in tumours of non-prostatic origin. These include breast and a minority of salivary gland tumours. The
antigen is a 33-34 kD glycoprotein that is restricted to epithelial cells of the prostate. An immunohistochemical study showed more
than 95% of prostatic carcinomas stained with anti-PSA8. PSA is demonstrable in the cytoplasm of acinar and ductal cells of normal or
malignant prostate tissue.
References
1. Gallee MPW, et al., Prostate. 9:33-45, 1986.
2. Nadji M, et al., Cancer. 48(5):1229-1232, 1981.
3. Genega EM, et al., Mod Pathol. 13(11):1186-91, 2000.
4. Green LK, et al., Hum Pathol. 22(3):242-6, 1991.
5. van Krieken JH, et al., Am J Surg Pathol. 17(4):410-4, 1993.
Ventana part number in black, Roche item number in blue. The products that appear on this page are RUO (for research use only).
184 NEXT>
Molecular Probes | IHC & ISH
Molecular Probes
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INFORM HER2 Dual ISH DNA Probe Cocktail Assay
Product
INFORM HER2 Dual ISH DNA Probe Cocktail
HybReady
ultraView SISH DNP Detection Kit
ultraView Siler Wash II
HER2 Dual ISH 3-in-1 Xenograft Slides
Part Number
800-4422
780-4409
800-098
800-505
780-003
783-4332
05899826001
05917557001
05907136001
05907128001
05446724001
05276101001
Quantity
25 tests
250 dispenses
100 tests
100 tests
2L
10 slides
Description
The INFORM HER2 Dual ISH DNA Probe Cocktail is designed to quantitatively detect amplification by light microscopy of the HER2
gene via two colour chromogenic in situ hybridisation (ISH) in formalin-fixed, paraffin-embedded tissue specimens of human breast
cancer and gastric cancer, including the gastro-oesophagal junction, following staining on VENTANA automated slide stainers. The
INFORM HER2 Dual ISH DNA Probe Cocktail is indicated as an aid in the assessment of patients for whom Herceptin (trastuzumab)
treatment is being considered.
This product should be interpreted by a qualified reader in conjunction with histological examination, relevant clinical information
and proper controls.
HER2 clusters and Chr17 60X, amplified
HER2 and Chr17 60X, non-amplified
References
1. Gschwind A, Fischer OM, Ullrich A. The discovery of receptor
tyrosine kinases: target for cancer therapy. Nat Rev Cancer
2004;4;361-370.
2. Muleris M, et al. Assignment of v-erb-b2 avian erythroblastic
leukemic viral oncogene homolog 2 (ERBB2) to human
chromosome band 17121.1 by in situ hybridisation. Cytogenet Cell
Genet. 1997;76:34-5.
3. Coussens L, Yang-Feng TL, Liao YC, Chen E, Gray A, McGrath
J, et al. Tyrosine kinase receptor with extensive homology to
EGF receptor shares chromosomal location with neu oncogene.
Science. 1985;230:1132-1139.
4. Slamon DJ, Godolphin W, Jones LA, et al. Studies of the
HER- 2/neu proto-oncogene in human breast and ovarian cancer.
Science. 1989;244:707-712.
5. Slamon DJ, Clark GM, Wong SG, et al. Human breast cancer:
correlation of relapse and survival with amplification of the
HER- 2/neu oncogene. Science 1987;235:177-182.
6. Narita M, Nakao K, Ogino N, et al. Independent prognostic factors in
breast cancer patients. Am J Surg 1998;175:73-75.
7. O’Reilly SM, Barnes DM, Camplejohn RS, et al. The relationship
between c-erbB-2 expression, S-phase fraction and prognosis in
breast cancer. Br J Cancer. 1991;63:444-446.
8. Pegram MD, Finn RS, Arzoo K, et al. The effect of HER-2/neu
overexpression on chemotherapeutic drug sensitivity in human breast
and ovarian cancer cells. Oncogene. 1997;15:537-547.
9. Press MF, Pike MC, Chazin VR, et al. HER-2/neu expression in nodenegative breast cancer: Direct tissue quantitation by computerised
image analysis and association of overexpression with increased risk of
recurrent disease. Cancer Res. 1993;53:4960-4970.
IHC & ISH | Molecular Probes
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VENTANA HER2 DNA Probe Assay
Product
VENTANA HER2 DNA Probe
INFORM Chromosome 17 Probe
Rabbit Anti-DNP
ultraView Alkaline Phosphatase (AP) Red ISH Detection Kit
HER2 3-in-1 Xenograft Control Slides
Interpretation Guide
Part Number
Quantity
780-4495
05999588001
50 tests
780-4331
05273412001
50 tests
780-4335
05273447001
100 tests
780-001
05271967001
100 tests
800-504
05278929001
100 tests
780-003
05276101001
2L
783-4332
05276101001
10 slides
Please contact your sales representative
Description
VENTANA HER2 DNA Probe is designed to detect amplification of the HER2 gene via chromogenic silver or dual colour in situ hybridisation
in formalin-fixed, paraffin-embedded breast and gastric cancer, including the gastro-oesophageal junction, tissue specimens following
staining on VENTANA automated slide stainers using light microscopy.
VENTANA HER2 DNA Probe spans approximately 200,000 base pairs of the HER2 gene (also known as ERBB2 and NEU). The DNP labelled
probe is designed to detect amplification of the HER2 gene, which occurs in a percentage of patients diagnosed with invasive breast
carcinoma.1 HER2/neu is a member of the family of four transmembrane receptor tyrosine kinases that mediate the growth, differentiation
and survival of cells. 2,3 The HER2 gene is located on Chromosome 17 and encodes the HER2 protein. 3,4 Overexpression of the HER2
protein, amplification of the HER2 gene or both occur in approximately 15 to 25 percent of breast cancers and are associated with
aggressive tumour behaviour.5,1
Recently, amplification of HER2 has been shown to occur in approximately 17% of invasive gastric tumours.6 Additionally, a high
correlation has been shown between HER2 IHC and ISH diagnostic assays on gastric tumour tissue. The scoring criteria for HER2 IHC
and ISH assays was previously published and differs slightly from the breast tumour criteria.7 Therefore, a knowledge of HER2 gene and/
or protein status in invasive breast or gastric cancer patients enables clinicians to make more informed decisions to improve the overall
management of their cancer patients.
VENTANA HER2 DNA Probe is indicated as an aid in the assessment of patients for whom Herceptin (trastuzumab) treatment is being
considered.
VENTANA Chromosome 17 Probe is designed to detect ploidy status of Chromosome 17 via chromogenic silver or dual colour in situ
hybridisation in formalin-fixed, paraffin-embedded human breast or gastric tissue specimens following staining on a VENTANA automated
slide stainer.
The determination of the genomic status of chromosome 17 (i.e., ploidy) plays a role in the assessment of patients for whom Herceptin
(trastuzumab) treatment is being considered. The VENTANA Chromosome 17 Probe is a 42 base-pair synthetic oligonucleotide designed
to “paint” the centromeric region of human Chromosome 17. The oligonucleotide probe also has a 5’ non-binding AT-rich tail that contains
DNP molecules detectable with anti-DNP antibodies.
References
1. Slamon DJ, Godolphin W, Jones LA, et al. Studies of the HER-2/
neu proto-ongene in human breast and ovarian cancer. Science
1989;244:707-712.
2. Gschwind A, Fischer OM, Ullrich A. The discovery of receptor
tyrosine kinases: target for cancer therapy. Nat Rev Cancer
2004;4;361-370.
3. Muleris M, et al., Assignment of v-erb-b2 avian erythroblastic
leukemic viral oncogene homolog 2 (ERBB2) to human chromosome
band 17121.1 by in situ hybridisation. Cytogenet Cell Genet 1997;
76:34-5.
4. Coussens L, Yang-Feng TL, Liao Y-C, chen E, Gray A, McGrath
et al. Tyrosine kinase receptor with extensive homology to EGF
receptor shares chromosomal location with neu oncogene. Science
1985;230:1132-1139.
186 NEXT>
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INFORM EGFR Probe Assay
Product
INFORM EGFR DNA Probe
Rabbit Anti-DNP
EGFR ISH Xenograft Control Slides
Part Number
800-4343
05278902001
800-4342
05278899001
780-4335
05273447001
780-001
05271967001
800-504
05278929001
780-003
05276101001
783-4343
05276110001
EGFR
Case 1: Single staining (silver) of EGFR and Chromosome 7
Chromosome 7
Quantity
25 tests
25 tests
100 tests
100 tests
100 tests
2L
10 slides
EGFR/Chromosome 7
Case 2: Dual staining of EGFR
(Silver) and Chromosome 7 (Red)
Description
The INFORM EGFR DNA Probe is designed to quantitatively detect EGFR genomic gain via chromogenic silver in situ hybridisation (SISH) in
formalin-fixed, paraffin-embedded human non-small cell lung cancer (NSCLC) specimens following staining on VENTANA automated slidestaining instruments using light microscopy.
EGFR gene status is reported as an absolute copy number as well as a function of the ratio of the average number of copies of the EGFR
gene to the average number of copies of Chromosome 7 (Chr7) per cell.
Visualisation of the EGFR gene and Chromosome 7 centromere is either performed on separate slides using the Silver in situ Hybridisation
(SISH) detection kit, or on one single slide, with a dual colour staining technique using SISH detection kit for the EGFR gene and Alkaline
Phosphatese (AP) Red ISH detection kit for Chromosome 7 centromere.
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INFORM IGF1R Probe Assay
Product
INFORM IGF1R DNA Probe
INFORM Chromosome 15 DIG Probe
HybReady
ultraView SISH DNP
IGF1R ISH Xenograft Slides
Case 1: Not amplified for IGF1R
(IGF1R in silver and Chr15 in red, 60X)
Part Number
800-4458
05994934001
800-4459
05994926001
780-4409
05917557001
800-098
05907136001
800-505
05907128001
780-003
05276101001
783-4458
05994977001
Quantity
25 tests
25 tests
250 dispenses
100 tests
100 tests
2L
10 slides
Case 2: Amplified for IGF1R
(IGF1R in silver and Chr15 in red, 60X)
Description
INFORM IGF1R DNP Probe is a Dinitro-Phenol (DNP) labelled probe designed to quantitatively detect IGF1R genomic gain via chromogenic
silver in situ hybridisation (SISH) using light microscopy in formalin-fixed, paraffin-embedded human tissue specimens following staining
on VENTANA automated slide stainer instruments. Genomic gain of IGF1R is associated with non-small cell lung cancer (NSCLC). The
IGF1R gene is located on chromosome 15 (15q26.3) and encodes the insulin-like growth factor-1 receptor protein.
Visualisation of the IGF1R gene and Chromosome 15 centromere is either performed on separate slides using the Silver in situ hybridisation
(SISH) DNP detection kit or on one single slide, with a dual colour staining technique using SISH DNP detection kit for the IGF1R gene and
Red ISH DIG detection kit for Chromosome 15 centromere.
188 NEXT>
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INFORM MET Probe Assay
Product
INFORM MET DNA Probe
HybReady
Rabbit Anti-DNP
MET ISH Xenograft Control Slides
Case 1: Amplified for MET on non-small cell lung carcinoma
Part Number
800-4372
05575311001
800-4342
05278899001
780-4409
05917557001
780-4335
05273447001
780-001
05271967001
800-504
05278929001
780-003
05276101001
783-4342
05575303001
Quantity
25 tests
25 tests
250 dispenses
100 tests
100 tests
100 tests
2L
10 slides
Case 2: Amplified for MET on gastric carcinoma
(NSCLC)
Description
INFORM MET DNA Probe is a Dinitro-Phenol (DNP) labelled probe designed to quantitatively detect MET (Mesenchymal-epithelial
transition factor) genomic gain via chromogenic silver in situ hybridisation (SISH) using light microscopy in formalin-fixed, paraffinembedded human tissue specimens following staining on VENTANA automated slide stainer instruments. Genomic gain of MET is
associated with non-small cell lung cancer (NSCLC) and gastric cancer. INFORM MET DNA Probe is designed to hybridise to the MET
gene locus on chromosome 7 (q21-q31).
Visualisation of the MET gene and Chromosome 7 centromere is either performed on separate slides using the Silver in situ hybridisation
(SISH) detection kit or on one single slide, with a dual colour staining technique using SISH detection kit for the MET gene and Red ISH
detection kit for Chromosome 7 centromere.
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INFORM TOP2A Probe Assay
Product
INFORM TOP2A DNA Probe
HybReady
Rabbit Anti-DNP
TOP2A ISH Xenograft Control Slides
Case 1: Normal TOP2A
Part Number
800-4348
05584957001
780-4331
05273412001
780-4409
05917557001
780-4335
05273447001
780-001
05271967001
800-504
05278929001
780-003
05276101001
783-4348
05584965001
Quantity
25 tests
25 tests
250 dispenses
100 tests
100 tests
100 tests
2L
10 slides
Case 2: Amplified for TOP2A
Description
INFORM TOP2A DNA Probe is a Dinitro-Phenol (DNP) labelled probe designed to detect TOP2A amplification or deletion via chromogenic
silver in situ hybridisation (SISH) using light microscopy in formalin-fixed, paraffin-embedded human tissue specimens following staining
on VENTANA automated slide stainer instruments. Amplification and deletion of TOP2A has been associated with invasive breast cancer.
The TOP2A gene is located on chromosome 17 (17q21-22) and encodes the topoisomerase II alpha protein.
Visualisation of the TOP2A gene and Chromosome 17 centromere is either performed on separate slides using the Silver in situ
hybridisation (SISH) detection kit or on one single slide, with a dual colour staining technique using SISH detection kit for the TOP2A gene
and Red ISH detection kit for Chromosome 17 centromere.
190 NEXT>
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HPV Probes: Tissue-Based Application
INFORM HPV II Family 6 Probe (B)
Part Number
Quantity
Status
To be used with
800-2220 05278546001
50 Dispenses
IVD
ISH iVie w Blue Plus Detection Kit
HPV 3 in 1 System Control Slides
Description
INFORM HPV II Family 6 (B) contains a cocktail of labelled human papillomavirus (HPV) genomic probes. The probe cocktail has
demonstrated positive hybridisation to the following low risk genotypes: 6 and 11. INFORM HPV II Family 6 (B) Probe is to be used in
conjunction with ISH iVIEW Blue Plus Detection Kit and other reagents to stain paraffin embedded tissue on the VENTANA BenchMark
series automated slide stainers.
INFORM HPV III Family 16 Probe (B)
Part Number
Quantity
Status
To be used with
800-4295 05278856001
50 Dispenses
IVD
ISH iVie w Blue Plus Detection Kit
Description
INFORM HPV III Family 16 (B) contains a cocktail of labelled human papillomavirus (HPV) genomic probes. The intended targets are the
common genital HPV genotypes found to be associated with cervical cancer. The probe cocktail has demonstrated positive hybridisation
to the following genotypes: 16, 18, 31, 33, 35, 45, 51, 52, 56, 58 and 66. INFORM HPV III Family 16 (B) Probe is to be used in conjunction
with ISH iVIEW Blue Plus Detection Kit and other reagents to stain paraffin embedded tissue on the VENTANA BenchMark Series
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Other Molecular Probes
INFORM EBER Probe
Part Number
Quantity
Status
To be used with
800-2842 05278660001
50 Dispenses
IVD
ISH iView Blue Detection Kit
Description
INFORM EBER Probe is a fluorescein-labelled oligonucleotide probe intended to identify, by in situ hybridisation (ISH) and light microscopy,
cells expressing Epstein-Barr encoded RNA (EBER) in sections of formalin-fixed, paraffin-embedded tissue. INFORM EBER Probe
specifically hybridises to EBER RNA transcripts. Positive results aid in the identification of cells infected with Epstein-Barr virus.
INFORM Kappa Probe
Part Number
Quantity
Status
To be used with
800-2843 05278678001
50 Dispenses
IVD
Description
INFORM Kappa Probe is a fluorescein-labelled oligonucleotide probe intended to detect cytoplasmic mRNA for kappa immunoglobulin
light chain in formalin-fixed, paraffin-embedded tissue samples. INFORM Kappa Probe is typically used in conjunction with the INFORM
Lambda Probe for lambda immunoglobulin light chain. The combined assays assist distinction between polyclonal (kappa positive, lambda
positive mRNA) proliferations associated with benign reactive plasma cell and B-cell lymphoid proliferations; and monoclonal (either kappa
positive and lambda negative or kappa negative and lambda positive) proliferations associated with neoplastic plasma cell and B-cell
lymphoid proliferations.
References
1. Pringle JH, et al., J. Pathol. 162(3): 197-207,1990.
2. Beck RC, et al. Diagn. Mol. Pathol. 12:14-20, 2003.
3. Euscher E, J. Gynecol Pathol. 21: 383-390, 2002.2003-03-31.
4. College of American Pathologists Laboratory Accreditation Program, Anatomic Pathology Checklist, 2001.
INFORM Lambda Probe
Part Number
Quantity
Status
To be used with
800-2844 05278686001
50 Dispenses
IVD
Description
INFORM Lambda Probe is a fluorescein-labelled oligonucleotide probe intended to detect cytoplasmic mRNA for lambda immunoglobulin
light chain in formalin-fixed, paraffin-embedded tissue samples. INFORM Lambda Probe is typically used in conjunction with the INFORM
Kappa Probe for kappa immunoglobulin light chain. The combined assays assist distinction between polyclonal (kappa positive, lambda
positive mRNA) proliferations associated with benign reactive plasma cell and B-cell lymphoid proliferations; and monoclonal (either kappa
positive and lambda negative or kappa negative and lambda positive) proliferations associated with neoplastic plasma cell and B-cell
lymphoid proliferations.
References
1. Pringle JH, et al., J. Pathol. 162(3): 197-207,1990.
2. Beck RC, et al. Diagn. Mol. Pathol. 12:14-20, 2003.
3. Euscher E, J. Gynecol Pathol. 21: 383-390, 2002.2003-03-31.
4. College of American Pathologists Laboratory Accreditation Program, Anatomic Pathology Checklist, 2001.
192 NEXT>
Ancillary Reagents | IHC & ISH
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Ancillary Reagents
Amplification
Amplification Kit
Part Number
Quantity
IHC  ISH
760-080 05266114001
100 tests
Description
May be used in conjunction with VENTANA IHC detection kits to increase the signal intensity of weak staining mouse and rabbit primary
antibodies. Reagents are provided pre-diluted and ready-to-use for convenient operation on VENTANA BenchMark series automated slide
stainers.
Components: Amplifier A, Amplifier B.
OptiView Amplification Kit
Part Number
Quantity
760-099 06396518001
50 tests
IHC  ISH
Description
OptiView Amplification Kit can only be used with OptiView IHC DAB Kit and provides a significant increase in sensitivity without sacrificing
specificity. The OptiView Amplification kit builds on the proprietary, non-endogenous hapten system used in the OptiView base kit. The
exclusive OptiView software provides increased control over the OptiView Amplification kit components allowing optimisation in intensity
while minimising primary antibody incubation times.
Components: OptiView Amplifier, OptiView Amplification H2O2, OptiView Amplification Multimer.
Blockers
Endogenous Biotin Blocking Kit
Part Number
Quantity
760-050 05266092001
250 tests
IHC  ISH
Description
Endogenous Biotin Blocking Kit may be used to aid in the reduction of non-specific staining caused by endogenous biotin present in cells
and tissues. Blocker A specifically binds to endogenous biotin present in the tissue; Blocker B saturates the remaining binding sites of
Blocker A, thus reducing the non-specific staining. For use on VENTANA BenchMark series automated slide stainers.
Components: Blocker A, Blocker B.
IHC & ISH | Ancillary Reagents
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ISH Block
Part Number
Quantity
780-4461 05994918001
100 tests
IHC
<PREV
ISH 
Description
ISH Block is used to decrease non-specific hybridisation of nick-labelled probes during in situ hybridisation staining process. ISH Block
reagent may be used in conjunction with Ventana ISH probes and other accessory reagents to stain single colour slides on the VENTANA
BenchMark series automated slide stainers. Single, ready-to-use dispenser.
NeuVision Blocking Reagent
Part Number
Quantity
760-1028 05266220001
250 tests
IHC  ISH
Description
Ready-to-use protein blocking solution suitable for use with the NeuVision Nuclear Stain Kit. It is intended to be used in the preparation
of samples for interpretation by image analysis. This reagent may be used to aid in the reduction of non-specific staining. For use on
VENTANA BenchMark series automated slide stainers. Single, ready-to-use dispenser.
Bulk Solutions
Cell Conditioning 1 (CC1)
Part Number
Quantity
Format
950-124 05279801001
2L bottle
Ready to use
IHC  ISH 
Description
Pre-diluted solution optimised for use on the VENTANA BenchMark GX and VENTANA BenchMark XT automated slide stainers as a pretreatment
step in the processing of tissue samples for immunohistochemistry and in situ hybridisation reactions. CC1 is a tris-based buffer with a slightly
basic pH, which, at elevated temperatures is capable of disrupting the covalent bonds formed by formalin in tissue. Removing these bonds allows
renaturation of protein molecules and increases antibody accessibility. Often these changes result in significant gains in antibody binding and
improved signal to noise ratios.
ULTRA Cell Conditioning 1 (ULTRA CC1)
Part Number
Quantity
Format
950-224 05424569001
2L bottle
Ready to use
IHC  ISH 
Description
Pre-diluted solution optimised for use on the VENTANA BenchMark ULTRA automated slide stainer as a pretreatment step in the
processing of tissue samples for immunohistochemistry and in situ hybridisation reactions. ULTRA CC1 is a tris-based buffer with a slightly
basic pH, which, at elevated temperatures, is capable of hydrolising the covalent bonds formed by formalin in tissue. Removing these bonds
allows renaturation of protein molecules and increases antibody accessibility. Often these changes result in significant gains in antibody
binding and improved signal to noise ratios.
Cell Conditioning 2 (CC2)
Part Number
Quantity
Format
950-123 05279798001
1L bottle
Ready to use
IHC  ISH 
Description
Pre-diluted solution optimised for use on the VENTANA BenchMark GX and VENTANA BenchMark XT automated slide stainers as a
pretreatment step in the processing of tissue samples for immunohistochemistry and in situ hybridisation reactions. CC2 is a citrate buffer
at a slightly acidic pH, which, at elevated temperatures, is capable of disrupting the covalent bonds formed by formalin in tissue. Removing
these bonds allows renaturation of protein molecules and increases antibody accessibility. Often these changes result in significant gains
in antibody binding and improved signal to noise ratios.
The products that appear on this page are IVD (for in vitro diagnostic use). Ventana part number in black, Roche item number in blue.
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ULTRA Cell Conditioning 2 (ULTRA CC2)
Part Number
Quantity
Format
950-223 05424542001
1L bottle
Ready to use
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IHC  ISH 
Description
Pre-diluted solution optimised for use on the VENTANA BenchMark ULTRA automated slide stainer as a pretreatment step in the
processing of tissue samples for immunohistochemistry and in situ hybridisation reactions. ULTRA CC2 is a citrate buffer at a slightly
acidic pH, which, at elevated temperatures, is capable of hydrolysing the covalent bonds formed by formalin and formaldehyde in tissue.
Removing these bonds allows renaturation of protein molecules and increases antibody accessibility. Often these changes result in
significant gains in antibody binding and improved signal to noise ratios.
EZ Prep (10X)
Part Number
Quantity
Format
950-102 05279771001
2L bottle
Concentrate
IHC  ISH 
Description
Solution used for paraffin removal from tissue samples and to dilute 2X SSC during stringency washes during in situ hybridisation (ISH)
reactions carried out on the VENTANA BenchMark series automated slide stainers.
Liquid Coverslip (High Temperature)
Part Number
Quantity
Format
650-010 05264839001
2L bottle
Ready to use
IHC  ISH 
Description
Solution used as a barrier between the aqueous reagents and the air, which has been optimised for use on the VENTANA BenchMark and
VENTANA BenchMark XT automated slide stainers. This barrier prevents evapouration, thereby providing a stable aqueous environment
for the immunohistochemistry or in situ hybridisation reactions.
ULTRA LCS
Part Number
Quantity
Format
650-210 05424534001
2L bottle
Ready to use
IHC  ISH 
Description
Solution used as a barrier between the aqueous reagents and the air, which has been optimised for use on the VENTANA BenchMark ULTRA
automated slide stainer. This barrier prevents evapouration, thereby providing a stable aqueous environment for immunohistochemistry or in situ
hybridisation reactions.
Reaction Buffer (10X)
Part Number
Quantity
Format
950-300 05353955001
2L bottle
Concentrate
IHC  ISH 
Description
Buffered solution used to rinse slides between staining steps and to provide a stable aqueous environment for the immunohistochemistry
and in situ hybridisation (ISH) reactions carried out on VENTANA BenchMark series automated slide stainers.
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SSC (10X)
Part Number
Quantity
Format
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IHC  ISH 
950-110 05353947001
2L bottle
Concentrate
Description
Sodium citrate, sodium chloride buffer used for stringency washes and to rinse slides between staining steps, It provides a stable aqueous
environment for the in situ hybridisation reactions carried out on a VENTANA BenchMark series automated slide stainer. SSC (10X) needs
to be used at a final 2X working concentration for hybridisation reactions. The VENTANA automated slide stainer automatically further
dilutes the 2X SSC solution as needed for stringency washes.
ultraView Silver Wash II
Part Number
Quantity
Format
IHC  ISH 
780-003 05446724001
2L bottle
Ready to use
Description
ultraView Silver Wash II solution is used to rinse slides between staining steps and provide a stable aqueous environment for the in situ
hybridisation chromogen reaction carried out on VENTANA BenchMark series automated slide stainers when using ultraView SISH
Detection Kit.
Counterstains
Bluing Reagent
Part Number
Quantity
IHC  ISH 
760-2037 05266769001
250 tests
Description
Ready-to-use solution that can be applied after hematoxylin to change the hue of hematoxylin to a blue colour. For use on VENTANA
Hematoxylin
Part Number
Quantity
760-2021 05266726001
250 tests
IHC  ISH 
Description
A modified Gill’s hematoxylin intended for staining cellular nuclei on slides containing cells from frozen tissue, formalin fixed and paraffin
embedded tissue or cytologic preparations. For use on VENTANA BenchMark series automated slide stainers. Single, ready-to-use
dispenser.
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Hematoxylin II
Part Number
Quantity
790-2208 05277965001
250 tests
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IHC  ISH 
Description
A modified Mayer’s hematoxylin intended for staining cellular nuclei on slides containing cells from frozen tissue, formalin-fixed and paraffinembedded tissue or cytologic preparations. For use on VENTANA BenchMark series automated slide stainers. Single, ready-to-use dispenser.
NeuVision Nuclear Stain Kit
Part Number
Quantity
760-085 05266149001
250 tests
IHC  ISH
Description
For use with imaging applications. Reagents are provided prediluted and ready-to-use for convenient operation. For use on VENTANA
Components: NeuVision Nuclear Stain, NeuVision Bluing Reagent, NeuVision Nuclear Diluent.
Red Counterstain II
Part Number
Quantity
780-2218 05272017001
100 tests
IHC
ISH 
Description
Red Counterstain II provides pink background staining in nuclei and cytoplasm of formalin-fixed, paraffin-embedded tissue sections,
which is intended to aid microscopic bright field observation for chromogenic in situ hybridisation (ISH) reactions. For use on VENTANA
Diluents
Antibody Diluent
Part Number
Quantity
251-018 05261899001
100 ml bottle
IHC  ISH 
Description
Buffered, proteinaceous solution used to dilute rabbit and mouse antibodies.
HybReady Solution
Part Number
Quantity
780-4409 05917557001
250 dispenses
IHC
ISH 
Description
HybReady Solution is a formamide-based buffer used to dilute probes and to create appropriate hybridisation conditions on the slides for
in situ hybridisation assays on VENTANA BenchMark series automated slide stainers. Single, ready-to-use dispenser.
Enzymes
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Protease 1
Part Number
Quantity
760-2018 05266688001
250 tests
<PREV
IHC  ISH
Description
Ready-to-use endopeptidase (alkaline protease) of the serine protease family that cleaves protein in the tissue section, allowing primary
antibodies to recognise and bind target epitope(s). The reagent is intended for enzymatic digestion of tissue sections. With 0.5 units/ml
alkaline protease enzyme activity, Protease 1 is the strongest enzymatic pretreatment available for IHC. Single, ready-to-use dispenser.
Protease 2
Part Number
Quantity
760-2019 05266696001
250 tests
IHC  ISH
Description
alkaline protease enzyme activity, Protease 2 is the intermediate-strength enzymatic pretreatment available for IHC. Single, ready-to-use
dispenser.
Protease 3
Part Number
Quantity
760-2020 05266718001
250 tests
IHC  ISH
Description
alkaline protease enzyme activity, Protease 3 is the mildest enzymatic pretreatment available for IHC. Single, ready-to-use dispenser.
ISH Protease 1
Part Number
Quantity
780-4147 05273315001
200 tests
IHC
ISH 
Description
Endopeptidase (alkaline protease) of the serine protease family which is used in the in situ hybridisation process to permeabilise cell
membranes and remove protein that surrounds the target DNA or RNA sequences of interest. The reagent is intended for enzymatic
digestion of sections of formalin-fixed, paraffin-embedded tissue or whole cell preparations. With 0.5 units/ml alkaline protease enzyme
activity, ISH Protease 1 is the strongest enzymatic pretreatment available for ISH. Single, ready-to-use dispenser.
ISH Protease 2
Part Number
Quantity
780-4148 05273323001
200 tests
IHC
ISH 
Description
digestion of sections of formalin-fixed, paraffin-embedded tissue or whole cell preparations. With 0.1 units/ml alkaline protease enzyme
activity, ISH Protease 2 is the intermediate-strength enzymatic pretreatment available for ISH. Single, ready-to-use dispenser.
ISH Protease 3
Part Number
Quantity
780-4149 05273331001
200 tests
IHC
ISH 
Description
digestion of sections of formalin-fixed, paraffin embedded tissue or whole cell preparations. With 0.02 units/ml alkaline protease enzyme
activity, ISH Protease 3 is the mildest enzymatic pretreatment available for ISH. Single, ready-to-use dispenser.
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Negative Control Reagents
ISH Negative Control
Part Number
Quantity
780-2902 05272165001
50 tests
IHC
ISH 
Description
ISH Negative Control is to be used to assess the background noise within the specimen sample under investigation. Single, ready-to-use
dispenser.
Negative Control Probe
Part Number
Quantity
800-2847 05278716001
50 tests
IHC
ISH 
Description
Negative Control Probe consists of the tail sequence used in our oligonucleotide probes and may be used as a negative control to assess
the background noise within the specimen under investigation. Single, ready-to-use dispenser.
Positive Control Reagents
Alu Positive Control Probe
Part Number
Quantity
800-2845 05278694001
50 tests
IHC
ISH 
Description
Fluorescein-labelled oligonucleotide probe that has an affinity to human Alu specific sequences. Single, ready-to-use dispenser.
Alu Positive Control Probe II
Part Number
Quantity
780-2221 05272041001
50 tests
IHC
ISH 
Description
DNP-labelled probes to determine the preservation of DNA during specimen collection, processing, fixation and handling. Single, ready-touse dispenser.
RNA Positive Control Probe
Part Number
Quantity
800-2846 05278708001
50 tests
IHC
ISH 
Description
Fluorescein-labelled oligonucleotide probe that has an affinity to the poly-A tail in mRNA. Single, ready-to-use dispenser.
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Specimen/Control Slides
IHC  ISH
PATHWAY HER-2 4 in 1 Control Slides
Part Number
Quantity
781-2991 05273510001
10 slides
Description
PATHWAY HER-2 4 in 1 Control Slides consist of formalin fixed, paraffin embedded, cultured human breast cell lines and are intended to be
used as assayed, semi-quantitative quality control material, in conjunction with PATHWAY HER-2 (4B5) Primary Antibody, for use in monitoring
the performance of the immunohistochemical c-erbB-2/HER-2 staining process on the VENTANA BenchMark series automated slide stainers.
The clinical interpretation of any staining or the absence of staining, must be complemented by morphological studies and evaluation of proper
controls. Evaluation must be made by a qualified pathologist within the context of the patient’s clinical history and other diagnostic tests.
(300x)
The Level 0 Cell Line Control
(MCF-7) is completely negative.
(300x)
The Level 1+ Cell Line Control
(T-47D) stains at an intensity
level of 1+.
(300x)
The Level 2+ Control Cell
Line (MDA-MB-453) stains at
an intensity level of 2+ with
complete “ring” pattern in >10%
of the cells.
(300x)
The Level 3+ Cell Line
Control (BT-474) is a high
expression cell line that stains
at an intensity level of 3+ in
approximately 100% of the cells.
EBER Positive Specimen Slides
Part Number
Quantity
801-2842 05278953001
10 slides
IHC
ISH 
Description
EBER (Epstein-Barr Virus Encoded RNA) Positive Specimen Slides contain 10 slides for use as an unassayed control for the qualitative
determination of the in situ hybridisation (ISH) staining function on the VENTANA BenchMark series automated slide stainer. Each slide
contains two cell pellets, one negative and one positive for early RNA transcript of EBV infection, fixed in 10% neutral buffered formalin
(NBF). Light microscopy is used to detect the staining reaction.
EGFR ISH Xenograft Control Slides
Part Number
Quantity
783-4343 05276110001
10 slides
IHC
ISH 
Description
EGFR ISH Xenograft Control Slides are intended to be used as assayed, semi-quantitative quality control material in conjunction with the
INFORM® EGFR DNA Probe and INFORM® Chromosome 7 Probe for use in monitoring performance of the probes for in situ hybridisation
(ISH). Light microscopy is used to detect the staining reaction. Two human carcinoma cell lines with different EGFR gene status are
generated as tumours (xenografts) in SCID mice. The two xenografts are paraffin embedded, sectioned and placed on charged slides.
HER2 DUAL ISH 3-in-1 Xenograft Slides
Part Number
Quantity
783-4422 05640300001
10 slides
IHC
ISH 
Description
HER2 Dual ISH 3-in-1 Xenograft Slides are intended to be used for initial assay installation and/or troubleshooting activities in conjunction
with the INFORM HER2 Dual ISH DNA Probe Cocktail on the VENTANA BenchMark series automated slide stainer.
200 NEXT>
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HER2 3-in-1 Xenograft Control Slides
Part Number
Quantity
783-4332 05276101001
10 slides
IHC
<PREV
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ISH 
Description
HER2 3-in-1 Xenograft Control Slides are intended to be used as assayed, semi-quantitative quality control material in conjunction with the
INFORM HER2 DNA Probe and INFORM Chromosome 17 Probe for use in monitoring performance of the probes for in situ hybridisation
(ISH). Light microscopy is used to detect the staining reaction. Three different human carcinoma cell lines, each with a different HER2
gene status that represents the dynamic range, are generated as tumours (xenografts) in SCID mice. The three xenografts are paraffin
embedded, sectioned and placed on charged slides.
Part Number
Quantity
783-2219 05273587001
10 slides
IHC
ISH 
Description
HPV 3 in 1 System Control Slides are used to monitor the performance of automated HPV in situ hybridisation staining assays.
Established controls, such as HPV 3 in 1 System Control Slides, should be processed and run with all clinical specimens. The
CaSki section is a cell line that reportedly contains approximately 200 - 400 copies of human papillomavirus (HPV) type 16 per
cell and the HeLa section is a cell line that reportedly contains approximately 10 - 50 copies of HPV type 18 per cell. The C-33A
negative section is a cell line which is an HPV negative human cervical carcinoma.
HPV Family 6 System Control Slides (B)
Part Number
Quantity
782-2839 05273528001
10 slides
IHC
ISH 
Description
Each HPV Family 6 System Control Slides (B) contains one formalin-fixed, paraffin-embedded condyloma tissue section. HPV
Family 6 System Control Slides (B) are designed to allow the user to check ISH staining function on VENTANA BenchMark series
IGF1R ISH Xenograft Slides
Part Number
Quantity
783-4458 05994977001
10 slides
IHC
ISH 
Description
IGF1R ISH Xenograft Slides are intended to be used for initial assay installation and/or troubleshooting activities in conjunction with the
INFORM IGF1R DNP Probe and INFORM Chromosome 15 DIG Probe on VENTANA BenchMark series automated slide stainers.
Kappa Positive Specimen Slides
Part Number
Quantity
801-2843 05278961001
10 slides
IHC
ISH 
Description
Kappa Positive Specimen Slides contain 10 slides for use as an unassayed control for the qualitative determination of the in situ
hybridisation (ISH) staining function on the VENTANA BenchMark series automated slide stainer. Each slide contains one paraffinembedded, 10% neutral buffered formalin (NBF) fixed tissue section of normal human tonsil. Slides are designed to allow the user to
confirm detection of kappa immunoglobulin light chain messenger RNA (mRNA) in plasma cells. Light microscopy is used to detect the
staining reaction.
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Lambda Positive Specimen Slides
Part Number
Quantity
801-2844 05278970001
10 slides
IHC
<PREV
ISH 
Description
Lambda Positive Specimen Slides contain 10 slides for use as an unassayed control for the qualitative determination of the in situ
hybridisation (ISH) staining function on the VENTANA BenchMark series automated slide stainer. Each slide contains one paraffinembedded, 10% neutral buffered formalin (NBF) fixed tissue section of normal human tonsil. Slides are designed to allow the user to
confirm detection of lambda immunoglobulin light chain messenger RNA (mRNA) in plasma cells. Light microscopy is used to detect the
staining reaction.
MET ISH Xenograft Control Slides
Part Number
Quantity
783-4472 05575303001
10 slides
IHC
ISH 
Description
MET ISH Xenograft Slides are intended to be used for initial assay installation and/or troubleshooting activities in conjunction with the
INFORM MET DNA Probe and INFORM Chromosome 7 Probe on VENTANA BenchMark series automated slide stainers.
TOP2A ISH Xenograft Control Slides
Part Number
Quantity
783-4348 05584965001
10 slides
IHC
ISH 
Description
The TOP2A ISH Xenograft Control Slides are designed to act as a system-level control to demonstrate that the system of reagents is
functioning as intended. Light microscopy is used to detect the staining reaction. Three human carcinoma cell lines with different TOP2A
gene status are generated as tumours (xenografts) in SCID mice. The three xenografts are paraffin embedded, sectioned and placed on
charged slides.
202 NEXT>
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User-fillable
dispensers/Prep Kits
50 Test Prep Kits
Product
50 Test Prep Kit # 501
Part Number
783-3457 05275814001
783-3458 05275822001
783-3459 05275849001
783-3460 05275857001
783-3461 05275865001
783-3462 05275873001
783-3463 05275881001
783-3464 05275890001
783-3465 05275903001
783-3466 05275911001
783-3467 05275920001
783-3468 05275938001
783-3469 05275946001
783-3470 05275954001
783-3471 05275962001
783-3472 05275989001
783-3473 05275997001
783-3474 05276004001
783-3475 05276012001
783-3476 05276039001
783-3477 05276047001
783-3478 05276055001
783-3479 05276063001
783-3480 05276071001
783-3481 05276080001
100 Test Prep Kits
Product
Part Number
783-2851 05273617001
783-2852 05273625001
783-2853 05273633001
783-2854 05273641001
783-2855 05273650001
783-2856 05273668001
783-2857 05273676001
783-2858 05273684001
783-2859 05273692001
Product
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Part Number
783-2860 05273706001
783-2861 05273714001
783-2862 05273722001
783-2863 05273749001
783-2864 05273757001
783-2865 05273765001
783-2866 05273773001
783-2867 05273781001
783-2868 05273790001
783-2869 05273803001
783-2870 05273811001
783-2871 05273820001
783-2872 05273838001
783-2873 05273846001
783-2874 05273854001
783-2875 05273862001
783-2876 05273889001
783-2877 05273897001
783-2878 05273919001
783-2879 05273927001
783-2880 05273935001
783-2881 05273943001
783-2882 05273951001
783-2883 05273960001
783-2884 05273978001
783-2885 05273986001
783-2886 05273994001
783-2887 05274001001
783-2888 05274010001
783-2889 05274028001
783-2890 05274036001
783-2891 05274044001
783-2892 05274052001
783-2893 05274079001
783-2894 05274087001
783-2895 05274095001
783-2896 05274109001
783-2897 05274117001
783-2898 05274125001
783-2899 05274133001
783-2900 05274141001
783-3007 05274168001
783-3008 05274176001
HOME
Product
Part Number
783-3009 05274184001
783-3010 05274192001
783-3011 05274206001
783-3012 05274214001
783-3013 05274222001
783-3014 05274249001
783-3015 05274257001
783-3016 05274265001
783-3017 05274273001
783-3018 05274281001
783-3019 05274290001
783-3020 05274303001
783-3021 05274311001
783-3022 05274320001
783-3023 05274338001
783-3024 05274346001
783-3025 05274354001
783-3026 05274362001
783-3027 05274389001
783-3028 05274397001
783-3029 05274419001
783-3030 05274427001
783-3031 05274435001
783-3032 05274443001
783-3033 05274451001
783-3034 05274460001
783-3035 05274478001
783-3036 05274486001
783-3037 05274494001
783-3038 05274508001
783-3039 05274516001
783-3040 05274524001
783-3041 05274532001
783-3042 05274559001
783-3043 05274567001
783-3044 05274575001
783-3045 05274583001
783-3046 05274591001
783-3047 05274605001
783-3048 05274613001
783-3049 05274621001
783-3050 05274630001
Product
Part Number
783-3051 05274648001
783-3052 05274656001
783-3053 05274664001
783-3054 05274672001
783-3055 05274699001
783-3056 05274702001
783-3057 05274729001
783-3058 05274737001
783-3059 05274745001
783-3060 05274753001
783-3061 05274761001
783-3062 05274770001
783-3063 05274788001
783-3064 05274796001
783-3065 05274800001
783-3066 05274818001
783-3067 05274826001
783-3068 05274834001
783-3069 05274842001
783-3070 05274869001
783-3071 05274877001
783-3072 05274885001
783-3073 05274893001
783-3074 05274907001
783-3075 05274915001
783-3076 05274923001
783-3077 05274931001
783-3078 05274940001
783-3079 05274958001
783-3080 05274966001
783-3081 05274974001
783-3082 05274982001
783-3083 05275008001
783-3084 05275016001
783-3085 05275024001
783-3086 05275032001
783-3087 05275059001
783-3088 05275067001
783-3089 05275075001
783-3090 05275083001
783-3091 05275091001
783-3092 05275105001
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Product
Part Number
783-3093 05275113001
783-3094 05275121001
783-3095 05275130001
783-3096 05275148001
783-3097 05275156001
783-3098 05275164001
783-3099 05275172001
783-3100 05275199001
783-3101 05275202001
783-3102 05275229001
783-3103 05275237001
783-3104 05275245001
783-3105 05275253001
783-3106 05275261001
783-3107 05275270001
783-3108 05275288001
783-3109 05275296001
783-3110 05275300001
783-3111 05275318001
783-3112 05275326001
783-3113 05275334001
783-3114 05275342001
783-3115 05275369001
783-3116 05275377001
783-3117 05275385001
783-3118 05275393001
783-3119 05275407001
783-3120 05275415001
783-3121 05275423001
783-3122 05275431001
783-3123 05275440001
783-3124 05275458001
783-3125 05275466001
783-3126 05275474001
783-3127 05275482001
783-3128 05275504001
783-3129 05275512001
783-3130 05275539001
783-3131 05275547001
783-3132 05275555001
783-3133 05275563001
783-3134 05275571001
Product
<PREV
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Part Number
783-3135 05275580001
783-3136 05275598001
783-3137 05275601001
783-3138 05275610001
783-3139 05275628001
783-3140 05275636001
783-3141 05275644001
783-3142 05275652001
783-3143 05275679001
783-3144 05275687001
783-3145 05275695001
783-3146 05275709001
783-3147 05275717001
783-3148 05275725001
783-3149 05275733001
783-3150 05275741001
783-3151 05275750001
783-3152 05275768001
783-3153 05275776001
783-3154 05275784001
783-3155 05275792001
783-3156 05275806001
250 Test Prep Kits
Product
Part Number
786-2851 05276284001
786-2852 05276292001
786-2853 05276306001
786-2854 05276314001
786-2855 05276322001
786-2856 05276349001
786-2857 05276357001
786-2858 05276365001
786-2859 05276373001
786-2860 05276381001
786-2861 05276390001
786-2862 05276403001
786-2863 05276411001
786-2864 05276420001
786-2865 05276438001
786-2866 05276446001
786-2867 05276454001
HOME
Product
Part Number
786-2868 05276462001
786-2869 05276489001
786-2870 05276497001
786-2871 05276519001
786-2872 05276527001
786-2873 05276535001
786-2874 05276543001
786-2875 05276551001
786-2876 05276560001
786-2877 05276578001
786-2878 05276586001
786-2879 05276594001
786-2880 05276608001
786-2881 05276616001
786-2882 05276624001
786-2883 05276632001
786-2884 05276659001
786-2885 05276667001
786-2886 05276675001
786-2887 05276683001
786-2888 05276691001
786-2889 05276705001
786-2890 05276713001
786-2891 05276721001
786-2892 05276730001
786-2893 05276748001
786-2894 05276756001
786-2895 05276764001
786-2896 05276772001
786-2897 05276799001
786-2898 05276802001
786-2899 05276829001
786-2900 05276837001
786-3007 05276845001
786-3008 05276853001
786-3009 05276861001
786-3010 05276870001
786-3011 05276888001
786-3012 05276896001
786-3013 05276900001
786-3014 05276918001
786-3015 05276926001
786-3016 05276934001
Product
Part Number
786-3017 05276942001
786-3018 05276969001
786-3019 05276977001
786-3020 05276985001
786-3021 05276993001
786-3022 05277019001
786-3023 05277027001
786-3024 05277035001
786-3025 05277043001
786-3026 05277051001
786-3027 05277060001
786-3028 05277078001
786-3029 05277086001
786-3030 05277094001
786-3031 05277108001
786-3032 05277116001
786-3033 05277124001
786-3034 05277132001
786-3035 05277159001
786-3036 05277167001
786-3037 05277175001
786-3038 05277183001
786-3039 05277191001
786-3040 05277205001
786-3041 05277213001
786-3042 05277221001
786-3043 05277230001
786-3044 05277248001
786-3045 05277256001
786-3046 05277264001
786-3047 05277272001
786-3048 05277299001
786-3049 05277302001
786-3050 05277329001
786-3051 05277337001
786-3052 05277345001
786-3053 05277353001
786-3054 05277361001
786-3055 05277370001
786-3056 05277388001
786-3057 05277396001
786-3058 05277400001
786-3059 05277418001
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Product
Part Number
786-3060 05277426001
786-3061 05277434001
786-3062 05277442001
786-3063 05277469001
786-3064 05277477001
786-3065 05277485001
786-3066 05277493001
786-3067 05277507001
786-3068 05277515001
786-3069 05277523001
786-3070 05277531001
786-3071 05277540001
786-3072 05277558001
786-3073 05277566001
786-3074 05277574001
786-3075 05277582001
786-3076 05277604001
786-3077 05277612001
786-3078 05277639001
786-3079 05277647001
786-3080 05277655001
786-3081 05277663001
786-3082 05277671001
786-3083 05277680001
Product
<PREV
NEXT>
Part Number
786-3084 05277698001
786-3085 05277701001
786-3086 05277710001
786-3087 05277728001
786-3088 05277736001
786-3089 05277744001
786-3090 05277752001
786-3091 05277779001
786-3092 05277787001
786-3093 05277795001
786-3094 05277809001
786-3095 05277817001
786-3096 05277825001
786-3097 05277833001
786-3098 05277841001
786-3099 05277850001
786-3100 05277868001
786-3101 05277876001
786-3102 05277884001
786-3103 05277892001
786-3104 05277906001
786-3105 05277914001
786-3106 05277922001
HOME
Fixative dispensers
Counterstain dispensers
Product
250 Test Counterstain # 1
Part Number
771-741
05271720001
771-742
05271738001
771-743
05271746001
771-744
05271754001
771-745
05271762001
771-746
05271789001
771-747
05271797001
771-748
05271819001
771-749
05271827001
771-750
05271835001
Enzyme dispensers
Product
250 Test Enzyme # 1
250 Test Enzyme # 2
250 Test Enzyme # 3
250 Test Enzyme # 4
250 Test Enzyme # 5
250 Test Enzyme # 6
250 Test Enzyme # 7
250 Test Enzyme # 8
250 Test Enzyme # 9
250 Test Enzyme # 10
Product
250 Test Fixative # 1
Part Number
771-731
771-732
771-733
771-734
771-735
771-736
771-737
771-738
771-739
771-740
05271614001
05271622001
05271649001
05271657001
05271665001
05271673001
05271681001
05271690001
05271703001
05271711001
Option dispensers
Part Number
771-721
771-722
771-723
771-724
771-725
771-726
771-727
771-728
771-729
771-730
05271517001
05271525001
05271533001
05271541001
05271550001
05271568001
05271576001
05271584001
05271592001
05271606001
Product
Part Number
250 Test Option # 1
250 Test Option # 2
250 Test Option # 3
250 Test Option # 4
250 Test Option # 5
250 Test Option # 6
250 Test Option # 7
250 Test Option # 8
250 Test Option # 9
250 Test Option # 10
771-751
771-752
771-753
771-754
771-755
771-756
771-757
771-758
771-759
771-760
05271843001
05271851001
05271860001
05271878001
05271886001
05271894001
05271908001
05271916001
05271924001
05271932001
Replacement filters
(20 Filters)
772-100
05271959001
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V ENTANA DIGITAL PATHOLOGY

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VENTANA Digital Pathology |
iScan Coreo Au Slide Scanner 213
Virtuoso Image Management Software 217
VENTANA DIGITAL PATHOLOGY

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iScan Coreo Au Slide Scanner | VENTANA DIGITAL PATHOLOGY

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iScan Coreo Au
Slide Scanner
The iScan Coreo Au slide scanner provides high quality brightfield slide
scanning with quality image for case review, sharing and telepathology.
Paired with our VENTANA Virtuoso software, iScan Coreo Au provides
users with a complete digital pathology solution. Image acquisition through
customised reporting increases operational efficiencies and productivity in
the lab.
I need to create a digital image archive.
• Access virtual image archives for case sharing
and collaboration
• Reduce slide handling errors
• Improve slide availability, portability
I need an effective way to review frozen sections.
• Use the “Live Mode” feature as a live microscope to view
tissue sections
• Enable the pathologist to render a review outside of the
surgery suite with remote viewing
• Reduce turnaround time
I need a solution that is easy to adopt
and implement into my lab.
• Benchtop scanner with a small footprint holding up to
160 slides
• Choose from multiple acquisition options to suit your
lab’s financial considerations
• Enjoy world-class training and support from our
industry-leading customer support organisation
• Implement and acclimate quickly with easy IT integration
VENTANA DIGITAL PATHOLOGY | iScan Coreo Au Slide Scanner

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Specifications
Computer
• Intel based PC, dual core, dual Xeon processor
• Storage: 160 SATA / 12 GB, CD/DVD Burner
• Connectivity: GIGABIT Ethernet ports
Computer monitor
• Resolution: flat panel high resolution monitor (1920x1200)
• Size: 24 inch
Input format
• 25 mm x 75 mm (1” x 3”) Microscope Slides
Slide capacity
• 1 to 160 slides using 8 standard Sakura baskets in an integrated rack
Automated turret
• Turret with support for four microscope objectives
Objectives
• Olympus 4X (0.1 NA), 10X (0.3 NA), 20X (0.5 NA) and 40X (0.75 NA)
Scanning resolution
• 2.5 μm/pixel at 4X, 1 μm/pixel at 10X, 0.46 μm/pixel at 20X, 0.23 μm/pixel at 40X
Barcode capability
• 1D and 2D barcode reading capability
Light source
• Integrated LED
Configuration
• Free standing
Light scan
• Automated barcode reading, tissue identification, auto focus, scanning and image compression
Manual scan
• User configurable scan area for single or batched slides in manual mode
LCD
• 5.5” diagonal full colour display 320 x 240 resolution
Scan viewing
• 24-bit true colour
Slide storage format
• Customisable to: JPEG 2000, BIF and TIFF
Features
• Only whole slide scanner with an automated turret with four objectives, allowing optical brightfield scanning at 4X, 10X, 20X and 40X
within one batch
• Performs volume scanning with up to 15 z-stacks
• Live Mode application supports remote microscopy; the iScan Coreo Au can be used as a live, digital microscope to remotely view frozen
sections at 1X-40X magnifications and multiple planes
• Walk-away automation with 160 slide capacity
Heat
Room Temperature
Humidity
Voltage
• 110-220 VAC
Output capacity
• 400 Watts (includes light source)
Frequency
• 50/60 Hz
Safety Certifications
Compliance
• CE, UL
Size (W x D x H)
• 111.76 x 84.07 x 158.50 cm (44.00” x 33.10” x 62.40”)
• 34 x 37 x 34 cm (13.5” x 14.5” x 13.5”)
Note: VENTANA products are for in vitro diagnostic use for specific applications and are intended for research use only for other applications.
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Better together |
Integrate with VENTANA VANTAGE workflow solution to track slides
from histology to slide scanning and diagnosis, for comprehensive real-time
visibility into your lab’s operation.
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Virtuoso Image Management Software | VENTANA DIGITAL PATHOLOGY

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Virtuoso
Image Management Software
Our advanced digital pathology application software supports slide viewing
and case management. Virtuoso web-based software manages the digitised
slide from scan to sign-out. By combining easy-to-use functionality and
a user-friendly interface, this application improves the efficiency and
productivity for the practicing pathologist. Virtuoso software and its rolebased portals can be easily adopted in your lab and will support multiple
users in telepathology, sharing and managing cases, viewing images and
much more for use with VENTANA slide scanners.
I need a way to review my cases remotely.
• Improve slide availability and portability with
digital archives
• Access cases remotely at any time, from anywhere, with
a web-based application
• View, manage and archive cases easily
I need a solution my entire lab can use effectively.
• Address the needs of each person in your workflow with
role-based portals
• Support the unique roles of pathologists,
histotechnologists, lab administrators and clinicians
• Improve efficiency within the entire pathology
workflow process
I need to share my cases for peer reviews and tumour
boards.
• Share cases with one or more pathologists, with the
ability to set user-defined levels of access on a case
• Reduce turnaround time by sharing slides digitally
• Eliminate glass slide logistics
• Use collaboration sessions to share and view case
images with colleagues in real time
VENTANA DIGITAL PATHOLOGY | Virtuoso Image Management Software

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Specifications
Unique Features
•
•
•
•
•
Quality control module allows the histotechnologist to review slide images prior to releasing them to the pathologist.
Side-by-side slide viewing and image synchronisation are available for image comparisons.
Intuitive user interface that mirrors pathology workflow allows users to re-arrange the order by which they view cases, specimens and images.
A true thin client option eliminates the need to install software.
Customise each individual user’s permissions according to individual roles in your lab’s daily workflow.
Virtuoso Minimum System Requirements
Client operation system
• Windows XP SP2
Supported browsers
• Internet Explorer 7.0 or 8.0, Mozilla Firefox 3.6.13
RAM
• 2 GB
Network Connectivity
• 10/100 Mbps Ethernet network card
Other software requirements • Adobe Acrobat Reader 8.0 for generating reports in PDF
Applied server specifications:
Operating system
• Windows 2008 SP2 64 bit
Processor
• Intel Dual Quad Core
RAM
• 16 GB
Network connectivity
• 1 GB Ethernet Network Card
Hard drive
• RAID 1/RAID 5 Configuration
• Hard Drives (2) 250 GB
• Hard Drives (3) 1 TB
Database
• MySQL 5.1 or Oracle v.11g Enterprise edition
Note: VENTANA products are for in vitro diagnostic use for specific applications and are intended for research use only for other applications.
Better together |
Digitise your glass slides using our iScan Coreo Au scanner, quantitate
slide images using our Companion Algorithm software and integrate with
VENTANA VANTAGE workflow solution to track slides from histology
to slide scanning and diagnosis, providing a comprehensive digital
pathology solution.
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Customer Services |
Technical Consultation Centre 223
Education and Training 227
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Technical Consultation Centre | CUSTOMER SERVICES

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Technical Consultation Centre
Our commitment is to provide world-class support in every interaction
with our customers. Our highly trained technical professionals offer realtime access to technical and scientific assistance in native English, French,
German, Italian and Spanish to support any customer needs.
Our vision
Our priority is to provide prompt and accurate technical
support, assist our customers with information and
troubleshooting on VENTANA instruments, reagents and
software and create continual positive experiences that
result from timely and effective problem resolution. The
Technical Consultation Centre works in conjunction with
our Investigation and Applications Laboratory to receive
technical updates and perform product quality verification.
Our goal is to maintain a peerless Customer Support
Centre that meets and ultimately exceeds customers’
expectations.
Additional information, product images, package inserts
and material safety data sheets are now available online for
your viewing and printing convenience.
Visit our website at www.ventana.com or www.roche.com
and follow the links to our online product catalogue.
CUSTOMER SERVICES | Technical Consultation Centre

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Technical Support
How to contact us:
Monday to Friday: 8:00 am – 5:00 pm (CET and GMT)
EMEA fax: +33 (0)4 76 18 51 43
Germany fax: +49 (0)69 97 20 35 00
By phone:
Country
224 Toll Phone
Toll Free Phone Email
Austria
+33(0)476761820
strasbourg.kundendienst@ventana.roche.com
Belgium
+33(0)476761822
Denmark
+33(0)476761826
strasbourg.tcceurope@ventana.roche.com
Finland
+33(0)476761816
France
+33(0)476761818
Meylan.TCCFrance@roche.com
Germany
+33(0)476761830
Italy
+33(0)476761832
Ireland
+33(0)476761817
Luxembourg
+33(0)476761818
Netherlands
+33(0)476761828
Northern Ireland
+33(0)476761817
Norway
+33(0)476761827
Poland (English)
+33(0)476761823
Portugal
+33(0)476761813
South Africa
+33(0)476761831
Spain
+33(0)476761812
Sweden
+33(0)476761829
Switzerland
+33(0)476761819
United Kingdom
+33(0)476761817
Other EMEA countries (ENGLISH)
+33(0)476761825
0 800 79 281
800 620 628
08007 833 119
Meylan.TCCItaly@roche.com
800 200 299
strasbourg.tccportugal@ventana.roche.com
902 112 476
0800 16 99 850
strasbourg.tccspain@ventana.roche.com
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Technical Consultation Centre | CUSTOMER SERVICES

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Education and Training | CUSTOMER SERVICES

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Education and Training
Our Training Centre demonstrates our ongoing commitment to providing
complete customer support.
At the Education and Training Centre, we are committed
to making your training as enjoyable and informative as
possible; our focus is on standardisation and improvement
of the slide-staining process, thereby helping technologists
deliver the best results daily in order to provide
pathologists with the best diagnostic information.
Training programmes, in small classes conducive to
efficient learning, are held throughout the year at our
comprehensive Training Centre located at our European
headquarters in Meylan (France), Mannheim (Germany)
and Burgess Hill (UK). All of our training courses are run
by trainers highly experienced in IHC or ISH techniques
and in VENTANA’s instruments and reagents.
Our objective is to provide you with the knowledge,
confidence and resources to enable effective optimisation
of experimental conditions for faster research and to
increase the productivity of your laboratory.
CUSTOMER SERVICES | Education and Training

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Instrument User Training Courses
228 Training Type
Objectives
Content
VENTANA BenchMark GX and
VENTANA BenchMark XT systems
Induction Part
3 days –
Meylan, Mannheim, Burgess Hill
F-TRN-107100
Attendees will experience a full
presentation of the instrument, the software
and all the IHC and ISH products.
The objective for each attendee is to be able
to use the instrument with all its capabilities
upon course completion.
Part of the training will be dedicated
to the instrument maintenance and
decontamination.
- Presentation of the VENTANA BenchMark GX and XT
systems: features and benefits
- Software presentation
- Maintenance and decontamination
- Function tests/service tests
- VENTANA products in IHC
- VENTANA products in ISH
- Error messages
VENTANA BenchMark ULTRA
system Induction Part
3 days –
F-TRN-107600
Attendees will experience a full
presentation of the instrument, the software
and all the IHC and ISH products.
The objective for each attendee is to be able
to use the instrument with all its capabilities
upon course completion.
Part of the training will be dedicated
to the instrument maintenance and
decontamination.
- Presentation of the VENTANA BenchMark ULTRA:
features and benefits
- Software presentation
- Maintenance and decontamination
- Function tests/service tests
- VENTANA products in IHC
- VENTANA products in ISH
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Advanced IHC / ISH Training Courses
Training Type
Objectives
Content
IHC Artefacts and their Resolution
1 day Meylan, Mannheim, Burgess Hill
F-TRN-109100
This course brings together all
the key parameters affecting
immunohistochemistry staining quality.
The training curriculum is focused on
a comprehensive understanding of the
basic theoretical principles of IHC and
on the resolution of the most common
artefacts.
THEORY
- Sample preparation: fixation, tissue processing, slide
preparation
- IHC staining techniques: detection principles
- Troubleshooting: definition of staining patterns &
artefacts and the possible causes
- Controls: positive & negative controls (definition and
sources of false positive/negative)
CASE STUDIES
- Presentations of several staining issues and how they
have been solved
IHC Protocol Set-up
and Optimisation
4 days –
F-TRN-107500
This course covers the theory of each
step in the IHC technique and all the
“protagonists” in the IHC reaction.
The participants will learn how to handle
the relevant information concerning
an antibody (pivotal reagent in IHC
staining).
The practical part of this course consists
in optimising staining protocols for given
antibodies on paraffin-embedded tissue
samples.
The staining result interpretation as well
as possible artefacts will be covered
during this in-house training session.
Finally, great interest will be focused
on the Quality Control in IHC, through
the use of control tissues (positive and
negative controls).
THEORY
- Tissue preparation: fixation, tissue processing, slide
preparation
- Antigen: proteins, localisation
- Pretreatment: heat, enzyme
- Antibody incubation: clone, sensitivity, specificity,
incubation time and temperature, working dilution
- Chromogenic detection: different detection
principles (LSAB, ABC, PAAP, Polymer, Multimer),
sensitivity
- Dual staining
- Controls: positive & negative controls (definition and
sources of false positive/negative)
- Staining Ancillaries: amplification kit, biotin blocker
kit, Neuvision reagents…
- Troubleshooting: definition of staining patterns and
artefacts.
PRACTICE
- Antibody technical datasheet: relevant information
monoclonal/ polyclonal, staining pattern, target,
control tissue, working dilution, tissue pretreatment,
storage conditions etc…
- Laboratory sessions:
• Perform runs with VENTANA and non-VENTANA
antibodies on slides brought by participants.
• Build a path to reach the optimal staining for a
given antibody on the corresponding positive
control.
CUSTOMER SERVICES | Education and Training

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Technical Training
These courses are provided to technical staff such as biomedical engineers having basic technical
knowledge. The course teaches participants how to install a VENTANA instrument and how to carry out a
basic troubleshooting analysis. Based on this diagnosis, they will be introduced to a list of possible causes
and repair possibilities (Level 1 Intervention).
Biomedical Engineer Training - Basic
This basic technical course provides a Biomedical Engineer with all the knowledge required to perform
basic repairs to a VENTANA instrument. At least two Biomedical Engineers are mandatory if the customer
chooses the Shared Maintenance agreement. This training can be conducted in one of our training centres
in Meylan (France), Burgess Hill (UK), Mannheim (Germany) or on the customer premises.
Biomedical Engineer Training - Advanced
For customers who would like to carry out all of the maintenance on a VENTANA instrument themselves,
this four-day course provides a Biomedical Engineer with the requisite level of expertise. This session must
be conducted in one of our training centres in Meylan (France) or Burgess Hill (UK).
Additional information on course content, dates and prices is available at:
Roche Diagnostics Meylan
Education and Training department
2 avenue du Vercors, BP 59, 38242 Meylan Cedex, France
Fax : +33 (0)4 76 76 46 60
E-mail : meylan.trainingRTD@roche.com
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Index |
INDEX |
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Index
A
C
a-1-Antichymotrypsin (ACT) (polyclonal) 68
a-1-Antitrypsin (AAT) (polyclonal) 68
a-1-Fetoprotein (AFP) (polyclonal) 69
AAT. See a-1-Antitrypsin 68
ACT. See a-1-Antichymotrypsin 68
ACTH. See Adrenocorticotropin 71
Actin, Muscle (HUC1-1) 69
Actin, Muscle Specific (HHF35) 70
Actin, Smooth Muscle (1A4) 70
Adrenocorticotropin (ACTH) (polyclonal) 71
AEC Detection Kit 49
AFB III Staining Kit 29
AFP. See a-1-Fetoprotein 69
Albumin, FITC 179
Alcian Blue for PAS 23
Alcian Blue Staining Kit 22
Alcian Yellow Staining Kit 29
ALK1 (ALK01) 71
Alu Positive Control Probe 199
Alu Positive Control Probe II 199
Amplification Kit 193
Annexin A1 (MRQ-3) 72
Antibody Diluent 197
Antibody Dilution Buffer 197
PATHWAY c-KIT (CD117) (9.7) 76
c-MET (SP44) 77
C1q, FITC 179
C3d (polyclonal) 77
C3, FITC 180
C4d (polyclonal) 78
C4, FITC 180
CA 19-9 (121SLE) 78
CA 125 (OC125) 79
Calcitonin (polyclonal) 79
Caldesmon (E89) 80
Calponin-1 (EP798Y) 57, 80
Calretinin (SP65) 81
Carcinoembryonic Antigen (CEA31) 81
CD1a (EP3622) 59, 64, 82
CD2 (MRQ-11) 82
CD3 (2GV6) 59, 82
CD4 (SP35) 83
CD5 (SP19) 65, 84
CD7 (SP94) 59, 65, 84
CD8 (SP57) 85
CD10 (SP67) 65, 85
CD14 (EPR3653) 86
CD15 (MMA) 86
CD19 (SP119) 87
CD20 (L26) 87
CD21 (2G9) 88
CD21 (EP3093) 88
CD22 (SP104) 89
CD23 (SP23) 65, 89
CD25 (4C9) 90
CD30 (Ber-H2) 90
CD31 (1A10) 91
CD31 (JC70) 91
CD34 (QBEnd/10) 92
CD43 (L60) 92
CD44 (SP37) 93
CD45 (LCA) (RP2/18) 93
CD45 (LCA) (2B11 & PD7/26) 94
CD45R (MB1) 94
CD45RO (UCHL-1) 95
CD56 (MRQ-42) 95
CD57 (NK-1) 96
CD61 (2f2) 96
CD63 (NKI/C3) 97
CD68 (KP-1) 97
CD71 (MRQ-48) 98
CD79a (SP18) 98
CD99 (O13) 99
CD117. See PATHWAY c-KIT 76
B
Basal Cell Cocktail (34βE12+4A4) 72
BCA-225 (Cu-18) 73
bcl-2 (124) 73
bcl-6 (GI191E/A8) 65, 74
BenchMark GX system 45-47
BenchMark XT system 41-43
BenchMark ULTRA system 37-39
Beta-Catenin (14) 57, 74
BG8, Lewisy (F3) 75
Blockers 193
Bluing Reagent 196
BOB.1 (SP92) 75
Breast Diagnostics 57
Bulk Solutions 194–195
234 CD138/syndecan-1 (B-A38) 99
CD163 (MRQ-26) 100
CDX-2 (EPR2764Y) 101
CEA. See Carcinoembryonic Antigen 81
Cell Conditioning 1 (CC1) 184
Cell Conditioning 2 (CC2) 194
Chromogranin A (LK2H10) 101
Collagen Type IV (CIV22) 102
Congo Red Staining Kit 31
Counterstains 196–197
COX-2 (Cyclooxygenase 2) (SP21) 103
Cyclin D1 (SP4-R) 65, 103
Cyclooxygenase 2. See COX-2 103
Cytokeratin (34βE12) 67, 104
Cytokeratin (AE1) 104
Cytokeratin (AE3) 105
Cytokeratin (CAM 5.2) 105
Cytokeratin (Pan) (AE1/AE3 & PCK26) 106
Cytokeratin 5/6 (D5/16B4) 57, 63, 67, 106
Cytokeratin 7 (SP52) 107
Cytokeratin 8 (35betaH11) 107
Cytokeratin 8 & 18 (B22.1 & B23.1) 108
Cytokeratin 14 (LL002) 109
Cytokeratin 19 (A53-B/A2.26) 110
D
Derm Diagnostics 59
Desmin (DE-R-11) 111
Detection chemistries 49-53
AEC Detection Kit 49
ISH iVIEW Blue Detection Kit 52
ISH iVIEW Blue Plus Detection Kit 52
iVIEW DAB Detection Kit 50
ultraView Alkaline Phosphatase (AP)
Red ISH Detection Kit 51
ultraView Red ISH DIG Detection Kit 51
ultraView SISH Detection Kit 53
ultraView SISH DNP Detection kit 53
ultraView Universal Alkaline
Phosphatase Red Detection Kit 51
ultraView Universal DAB Detection Kit 50
Diastase Kit 24
Diluents 197
DOG1 (SP31) 111
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| INDEX
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E
G
I
E-Bar II Barcode Slide Label System 39-43
EBER Positive Specimen Slides 200
INFORM EBER Probe 192
E-Cadherin (36) 112
E-Cadherin (EP700Y) 112
EGFR (3C6) 113
EGFR (5B7) 113
EGFR ISH Xenograft Control Slides 200
INFORM EGFR Probe Assay 187
Elastic Staining Kit 25
EMA (Epithelial Membrane Antigen) (E29) 114
Endogenous Biotin Blocking Kit 193
Enzymes 198
Ep-CAM/Epithelial Specific Antigen (Ber-EP4)
115
Epidermal Growth Factor Receptor. See EGFR
113
Epithelial Membrane Antigen. See EMA 114
Epithelial Related Antigen (MOC-31) 63, 116
Epithelial Specific Antigen. See Ep-CAM 115
ER. See Estrogen Receptor 117
ERG (EPR3864) 116
Estrogen Receptor (ER) (SP1) 117
EZ Prep (10X) 195
Galectin-3 (9C4) 120
Gastrin (polyclonal) 120
GCDFP-15 (EP1582Y) 57, 121, 178
GFAP (EP672Y) 121
GH (Polyclonal) 122
Giemsa Staining Kit 31
Glial Fibrillary Acidic Protein. See GFAP 121
Glucagon (polyclonal) 122
GLUT-1 (polyclonal) 123
Glycophorin A (GA-R2 & HIR2) 1213
Glypican-3 (1G12) 124
Glypican 3 (GC33) 124
GMS II Staining Kit 30
GMS Staining Kit 30
Granzyme B (polyclonal) 125
Growth Hormone. See GH 122
IgA, FITC 181
IgA (polyclonal) 132
IgD (polyclonal) 133
IGF1R ISH Xenograft Slides 201
INFORM IGF1R Probe Assay 188
IGF-1R. See Insulin Growth Factor-1 Receptor
IgG, FITC 181
IgG (polyclonal) 133
IgM, FITC 181
IgM (polyclonal) 133
Immunofluorescence antibodies 179–182
Immunoglobulin A. See IgA 181
Immunoglobulin G. See IgG 133
Immunoglobulin M. See IgM 133
Inhibin, alpha (R1) 134
Insulin (polyclonal) 135
Insulin Growth Factor-1 Receptor (G11) 135
Iron Staining Kit 32
iScan Coreo Au Slide Scanner 213-214
ISH Block 194
ISH iVIEW Blue Detection Kit 52
ISH iVIEW Blue Plus Detection Kit 52
ISH Negative Control 199
ISH Protease 1 198
ISH Protease 2 198
ISH Protease 3 199
iVIEW DAB Detection Kit 50
F
Factor VIII Related Antigen (polyclonal) 117
Factor XIIIa (AC-1A1) 118
Factor XIIIa (EP3372) 59, 118, 179
Fascin (55k-2) 119
Fibrinogen, FITC 180
FITC Primary Antibodies
Albumin, FITC 179
C1q, FITC 179
C3, FITC 180
C4, FITC 180
Fibrinogen, FITC 180
IgA, FITC 181
IgG, FITC 181
IgM, FITC 181
Kappa, FITC 182
Lambda, FITC 182
Follicle Stimulating Hormone. See FSH 119
FSH (polyclonal) 119
H
hCG. See Human Chorionic Gonadotropin 131
HBcAg. See Hepatitis B Virus Core Antigen 183
HBsAg. See Hepatitis B Virus Surface Antigen 183
Helicobacter pylori (polyclonal) 61, 125
Hematoxylin 22, 23, 24, 25, 26, 27, 33, 197
Hematoxylin II 197
Hepatitis B Virus Core Antigen (polyclonal) 183
Hepatitis B Virus Surface Antigen (S1-210) 183
Hepatocyte Specific Antigen. See HSA 130
PATHWAY HER-2 4 in 1 Control Slides 200
HER2 DUAL ISH 3-in-1 Xenograft Slides 201
PATHWAY HER-2/neu (4B5) 126, 127
VENTANA HER2 DNA Probe Assay 186
INFORM HER2 Dual ISH DNA Probe
Cocktail Assay 185
VENTANA HER-2/neu (4B5) 128
Herpes Simplex Virus I (polyclonal) 128
Herpes Simplex Virus II (polyclonal) 129
H&E staining 25
HGAL (MRQ-49) 129
HHV-8 (13B10) 130
hPL. See Human Placental Lactogen 131
HPV 3 in 1 System Control Slides 201
HPV Family 6 System Control Slides (B) 201
INFORM HPV II Family 6 Probe (B) 191
INFORM HPV III Family 16 Probe (B) 191
HSA (0CH1E5) 130
Human Chorionic Gonadotropin (polyclonal)
131
Human Herpes Virus Type 8. See HHV-8 130
Human Placental Lactogen (polyclonal) 131
HybReady Solution 197
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J
Jones H&E Staining Kit 25
Jones Light Green Staining Kit 26
K
Kappa (polyclonal) 135
Kappa, FITC 182
Kappa Positive Specimen Slides 202
INFORM Kappa Probe 192
Ki-67 (30-9) 136
Ksp-Cadherin (MRQ-33) 136
Keratin (34βE12). See Cytokeratin (34βE12) 104
Keratin (AE1). See Cytokeratin (AE1) 104
Keratin (AE3). See Cytokeratin (AE3) 105
Keratin (CAM 5.2). See Cytokeratin (CAM 5.2)
105
Keratin (Pan) (AE1/AE3 & PCK26). See
Cytokeratin (Pan) (AE1/AE3 & PCK26) 106
Keratin 5/6 (D5/16B4). See Cytokeratin 5/6
(D5/16B4) 106
Keratin 7 (SP52). See Cytokeratin 7 (SP52) 107
Keratin 8 (35betaH11). See Cytokeratin 8
(35betaH11) 107
INDEX |
HOME
Keratin 8 & 18 (B22.1 & B23.1). See Cytokeratin 8
& 18 (B22.1 & B23.1) 108
Keratin 14 (LL002). See Cytokeratin 14 (LL00) 109
Keratin 19 (A53-B/A2.26). See Cytokeratin 19
(A53-B/A2.26) 110
MUC1 (H23) 145
MUC2 (MRQ-18) 145
MUC5AC (MRQ-19) 146
MUC6 (MRQ-20) 147
Mucicarmine Staining Kit 22
MUM1 (MRQ-43) 65, 148
Myeloperoxidase (polyclonal) 148
Myogenin (F5D) 149
Myoglobin (polyclonal) 149
Myosin, Smooth Muscle (SMMS-1) 150
L
Lambda (polyclonal) 137
Lambda, FITC 182
Lambda Positive Specimen Slides 202
LCA. See CD45 94
LH. See Luteinizing Hormone 138
Light Green for PAS 24
Liquid Coverslip (High Temperature) 195
Liquid Coverslip (Low Temperature) 33
LMO2 (1A9-1) 137
Lung Diagnostics 63
Luteinizing Hormone (polyclonal) 132
Lymphoma Diagnostics 65
Lysozyme (polyclonal) 138
M
Macrophage (HAM-56) 139
Mammaglobin (31A5) 139
MART-1/melan A (A103) 140
Melanoma Associated Antigen (KBA.62) 59,
141
Melanoma Associated Antigen (PNL2) 141
Melanosome (HMB45) 142
Mesothelial Cell (HBME-1) 142
MET ISH Xenograft Control Slides 202
INFORM MET Probe Assay 159
MITF (C5/D5) 143
MLH1 (M1) 143
Molecular Probes 185
INFORM EBER Probe 192
INFORM EGFR Probe Assay 187
VENTANA HER2 DNA Probe Assay 186
INFORM HER2 Dual ISH DNA
Probe Cocktail Assay 185
INFORM HPV II Family 6 Probe (B) 191
INFORM HPV III Family 16 Probe (B) 191
INFORM IGF1R Probe Assay 188
INFORM Lambda Probe 192
INFORM MET Probe Assay 189
INFORM TOP2A Probe Assay 190
MPO. See Myeloperoxydase 148
MSH2 (G219-1129) 144
MSH6 (44) 144
236 N
Napsin A (Polyclonal) 150
Negative Control Mouse Ig (MOPC21) 151
Negative Control Probe 199
Negative Control Rabbit Ig 151
Negative Control Reagents 199
Nerve Growth Factor Receptor (MRQ-21) 151
Neurofilament (2F11) 152
Neuron Specific Enolase. See NSE 152
NeuVision Blocking Reagent 194
NeuVision Nuclear Stain Kit 197
NexES Special Stainer 19
Heavy Metal Extraction System 21
Vapor Extraction Filter 21
Waste Container with Sealed Cap 21
NGFR. See Nerve Growth Factor Receptor 151
NSE (E27) 152
O
Oct-2 (MRQ-2) 153
Oct-4 (MRQ-10) 154
OptiView Amplification Kit 193
OptiView DAB IHC Detection Kit 50
P
p21WAF1 (DCS-60.2) 154
p27kip1 (SX53G8) 155
p53 (Bp-53-11) 155
p53 (DO-7) 156
p63 (4A4) 57, 61, 156
p120 (98) 57, 157
Pan. See Cytokeratin (Pan) (AE1/AE3 & PCK26)
106
Parathyroid Hormone (MRQ-31) 157
PAS Staining Kit 23
PAX5 (SP34) 158
PD-1 (MRQ-22) 57, 159
Perforin (MRQ-23) 160
PGP 9.5 (polyclonal) 160
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PHH3. See Phosphohistone-H3 161
Phosphohistone-H3 (polyclonal) 161
Placental Alkaline Phosphatase (NB10) 161
PLAP. See Placental Alkaline Phosphatase 161
PMS2 (EPR3947) 162
Pneumocystis jiroveci (carinii) (3F6) 162
Podoplanin (D2-40) 163
Positive control reagents 189
PR. See Progesterone Receptor 163
Progesterone Receptor (1E2) 163
Prolactin (polyclonal) 164
Prostate Diagnostics 67
Prostate Specific Antigen. See PSA 22
Prostatic Acid Phosphatase. See PSAP 22
Protease 1 198
Protease 2 198
Protease 3 198
Protein Gene Product 9.5. See PGP 9.5 160
PSA (ER-PR8) 184
PSA (polyclonal) 164
PSAP (PASE/4LJ) 165
PTH. See Parathyroid Hormone 157
R
RCC. See Renal Cell Carcinoma 165
Reaction Buffer (10X) 195
Red Counterstain II 195
Renal Cell Carcinoma (RCC) (PN-15) 165
Reticulum II Staining Kit 26
S
S100 (4C4.9) 166
S100 (polyclonal) 166
Simian Virus 40. See SV40 168
Smoothelin (R4A) 167
Somatostatin (polyclonal) 163
Special Stains 19-30
AFB III Staining Kit 29
Alcian Blue for PAS 23
Alcian Blue Staining Kit 22
Alcian Yellow Staining Kit 29
Congo Red Staining Kit 31
Diastase Kit 24
Elastic Staining Kit 25
Giemsa Staining Kit 31
GMS II Staining Kit 30
GMS Staining Kit 30
Iron Staining Kit 32
Jones H&E Staining Kit 25
Jones Light Green Staining Kit 26
Light Green for PAS 24
Mucicarmine Staining Kit 22
PAS Staining Kit 23
Reticulum II Staining Kit 26
Steiner Staining Kit 30
Trichrome II Blue Staining Kit 27
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Trichrome III Blue Staining Kit 27
Trichrome III Green Staining Kit 28
Special Stains Cleaning Kit 33
Special Stains Wash (10x) 33
Specimen/Control Slides 200
EBER Positive Specimen Slides 200
EGFR ISH Xenograft Control Slides 200
HER2 3-in-1 Xenograft Control Slides 201
PATHWAY HER-2 4 in 1 Control Slides 200
HER2 DUAL ISH 3-in-1 Xenograft Slides 201
HPV 3 in 1 System Control Slides 201
HPV Family 6 System Control Slides (B) 201
IGF1R ISH Xenograft Slides 201
Kappa Positive Specimen Slides 202
Lambda Positive Specimen Slides 202
MET ISH Xenograft Control Slides 202
TOP2A ISH Xenograft Control Slides 202
Spectrin (RBC2/3D5) 168
SV 40 (MRQ-4) 168
SSC (10X) 196
Steiner Cleaning Kit 30
Steiner Staining Kit 30
SYMPHONY H&E system 13, 14, 15
OPTISURE Coverslips 15
SYMPHONY B 15
SYMPHONY C 15
SYMPHONY Clear 15
SYMPHONY D 15
SYMPHONY Desiccant Filter 15
SYMPHONY N1 15
SYMPHONY N2+ 15
SYMPHONY N2+ Cleaning Kit 15
SYMPHONY Tray Kit 15
SYMPHONY W 15
Synaptophysin (MRQ-40) 169
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T
V
T-bet (MRQ-46) 169
TAG-72 (B72.3) 57, 170
Tartrate-Resistant Acid Phosphatase. See TRAcP
173
TdT (polyclonal) 170
Terminal Deoxynucleotidyl Transferase. See TdT
170
Thymidine Phosphorylase (P-GF.44C) 171
Thyroglobulin (2H11+6E1) 171
Thyroid Stimulating Hormone (polyclonal) 172
Thyroid Transcription Factor-1 (8G7G3/1) 172
TOP2A ISH Xenograft Control Slides 202
INFORM TOP2A Probe Assay 190
Topoisomerase IIa (JS5B4) 173
TRAcP (9C5) 173
Trichrome II Blue Staining Kit 27
Trichrome III Blue Staining Kit 27
Trichrome III Green Staining Kit 28
Tryptase (G3) 174
TSH. See Thyroid Stimulating Hormone 172
TTF-1. See Thyroid Transcription Factor-1 172
Tyrosinase (T311) 174
VANTAGE. See VANTAGE workflow solution 3
VANTAGE workflow consulting 7
VANTAGE workflow management suite 4
VANTAGE workflow solution 3, 4, 15, 43, 48
VANTAGE Lean Workflow Solution 8
Villin (CWWB1) 175
Vimentin (V9) 176
Vimentin (Vim 3B4) 176
Virtuoso image management software 217–218
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W
Workflow Consulting 7, 8
WT1 (6F-H2) 63, 177
Z
ZAP-70 (2F3.2) 63, 177
U
ULTRA Cell Conditioning 1 (ULTRA CC1) 194
ULTRA Cell Conditioning 2 (ULTRA CC2) 194
ULTRA LCS 195
ultraView Red ISH DIG Detection Kit 51
ultraView Silver Wash II 196
ultraView SISH DNP detection kit 53
ultraView Universal Alkaline Phosphatase Red
Detection Kit 51
ultraView Universal DAB Detection Kit 50
Uroplakin III (SP73) 175
User-fillable dispensers/Prep Kits 230-208
50 Test Prep Kits 203
Counterstain Dispenser 208
Enzyme Dispensers 208
Fixative Dispensers 208
Option Dispensers 208
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238 <PREV
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Roche Diagnostics International Ltd.
CH-6343 Rotkreuz
Switzerland
www.roche.com
www.ventana.com
Roche Diagnostics
VENTANA product portfolio
© 2011 Ventana Medical Systems, Inc.
and Roche Diagnostics International Ltd.
VENTANA, the VENTANA logo, BENCHMARK,
CONFIRM, DISCOVERY, INFORM, NexES, OPTISURE,
PATHWAY, SYMPHONY, ultraView and VANTAGE are
trademarks of Roche.
All other trademarks are the properties
of their respective owners.
E2123 1011A
06562604001
third edition October, 2011
Roche Diagnostics VENTANA Product Portfolio | Third Edition