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Cellular Adaptation and Cell Injury
CLDavis Foundation
On the Beach
Lec 1
Cell Injury
Injury, Causes
Causes,
Responses, Reversible,
Necrosis
R K Myers 2009
Ron Myers, DVM PhD
DACVP
Professor Veterinary Pathology
Iowa State University
The “elemental
patient”
Rudolf Virchow (1821-1902)
1
2
Cellular responses to stress
• Many causes of cell injury
• Homeostasis, a more or less narrow range of
steady state, can be altered.
• Cellular adaptations can result in an altered
steady state with changes in morphology.
• Limited ways to respond to injury.
• The body is a fiendishly complicated and clever
aggregation of cells and the lesions resulting
from cell injury can be complex.
Cells can and must adapt to
stimulus or stress
• In response to stress, cells may:
– Adapt
– Be reversibly injured
– Die
hypertrophy
hyperplasia
metaplasia
atrophy
dysplasia
3
Responses to
Cell stress
Injurious agent
Normal Cell
Reversible
Lesion
Ad
Adaptation
i
Hypertrophy
Hyperplasia
Atrophy
Metaplasia
Dysplasia
Cell Death
Inadequate adaptation
Residual Lesion
Lipofuscin, etc.
The normal cell, adapted cell,
injured cell, irreversibly injured
cell, and dead cell are inexact states
along a continuum of function and
structure.
Causes of Cell Injury
• Extrinsic or Intrinsic.
• Oxygen deficiency—common and important
– Hypoxia reduces aerobic oxidative respiration
– Results from l) cardiorespiratory failure, 2) loss of
blood supply, 3) reduced transport of O2 in blood (i.e.
anemia,
i or CO ttoxicity),
i it ) and
d 4) bl
blockage
k
off cellll
enzymes (cyanide toxicosis).
– Compare to ischemia: loss of blood supply.
• Physical agents
– Trauma, extremes of heat and cold, radiation, and
electrical energy may seriously injure cells
4
Causes of Cell Injury
• Infectious agents
–
–
–
–
–
Viruses
Bacteria
Mycotic agents
Protozoa
Metazoan parasites
• Nutritional deficiency and imbalances
– Protein-calorie deficiencies
– Protein-calorie excess
– Vitamin and mineral imbalances
Causes of Cell Injury
• Genetic derangement
Mutations, whatever their origin, may cause no
disease, deprive a cell of a critical protein
(enzyme), or may be incompatible with cell
survival.
• Workload imbalance
– Overworked or under worked cells must adapt
Causes of Cell Injury
• Chemicals, drugs, toxins
– Influence cells by a multitude of mechanisms
– block or stimulate cell membrane receptors, alter specific
enzyme systems, produce toxic free radicals, alter cell
permeability, damage chromosomes, modify metabolic
pathways,
p
y , and damage
g structural components
p
of cells.
• Immune dysfunction
– Failure to respond due to congenital or acquired defects
– Autoimmunity -- Immune response directed against host
antigens
– Hypersensitivity -- inappropriate or exaggerated response to
certain antigens
5
The cellular response depends on:
•
•
•
•
•
Type of lesion
Duration and severity (high or low dose )
Type of cell lesion
Physiologic state of the cell
Capacity of the cell for adaptation
Important susceptible cell
components
• Generation of ATP by aerobic respiration.
• Integrity of the cell membrane (cellular
ionic and osmotic homeostasis))
• Protein synthesis
• Cytoskeleton
• Genetic integrity
Cellular and biochemical sites of damage in cell injury
•ATP decrease
•Mitochondrial damage
•Loss of calcium homeostasis
•Oxidative stress
6
Consequences of
decreased
intracellular ATP:
•Plasma membrane sodium
pump failure
•Calcium influx
•Altered
Altered energy metabolism
•Reduction in protein
synthesis
•Unfolded protein response
Mitochondrial
Damage by:
•Cytosolic Calcium influx
•Oxidative stress
•Phospholipase A2
breakdown of phospholipids
and sphingomyelin pathways
Mitochondrial
damage
g results in:
•Mitochondrial permeability
transition of inner
mitochondrial membrane
•Reversible but can become
permanent and be death
blow
•Leakage of cytochrome c
and initiation of apoptosis
•Loss of membrane
potential, no ATP, necrosis
Calcium influx from:
•Extracellular stores due to loss
of membrane function.
(Cytosolic free calcium very low
at <0.1 umol while extracellular
levels 1.3 mmol.)
•Intracellular Ca sequestered in
mitochondria and ER.
•Ca-Mg – ATPase pump
damage or increased
membrane permeability
Intracellular Calcium
results in:
•Enzyme activation
7
Oxygen-derived free radicals – Reactive oxygen species leading to oxidative stress
Free radicals initiated by:
Radiant energy: UV, X ray
Inflammation: e.g. neutrophil killing
Chemicals and drugs and their metabolism – eg chloroform
Redox reactions of normal metabolism
Transition metals: especially iron and copper, which donate or accept
free electrons
Nitric oxide (just say NO!)
Lipid peroxidation of
membranes:
ROS attack on unsaturated Fas of
membrane lipids yield peroxides
and autocatalyze chain reactions
(propagation)
Oxidative modification of
proteins:
Amino acid side chain oxidation,
cross linkages (disulfide bonds), and
protein backbone oxidation result in
fragmentation and increased
degradation by proteasome complex
DNA Lesions:
Thymine reaction in nuclear and
mitochondrial DNAÆsingle stranded
breaks (aging?)
8
Reactive oxygen species: brief summary
•
Hydrogen peroxide (H2O2)
– Forms free rads via Fe2+ catalyzed Fenton Reaction
– Diffuses widely in cell
•
Superoxide anion (O2-)
– Generated by leaks in electron transport chain and
some cytosolic reactions
– Produces other ROS
– Does not diffuse far from origin
Reactive oxygen species: brief summary
continued
•
Hydroxyl radical (.OH)
– Generated from H2O2 by Fe2+ catalyzed Fenton
reaction
– Intracellular radical most responsible for
macromolecule
l
l attack
tt k
•
Peroxynitrite (ONOO.)
– From the reaction of nitric oxide (NO) with O2– Damages macromolecules
Reactive oxygen species: brief summary
continued
• Lipid peroxide radicals (RCOO.)
– Organic radicals produced during lipid
peroxidation
• Hypochlorous acid (HOCl)
– Produced by macrophages and pmns during
respiratory burst accompanying phagocytosis
– Dissociates to yield hypochlorite radical (OCl-)
9
Protection from ROS:
Inherently unstable
Antioxidants block or scavenge
Vit. E and A
Binding of catalyzing iron and
copper to transport proteins (e.g.
transferrin, ceruloplasmin,
lactoferrin, ferritin)
En mes
Enzymes:
•Catalase (in peroxisomes)
decomposes hydrogen peroxide
•Superoxide dismutase converts
superoxide to water and oxygen
•Glutathione peroxidase catalyzes
free radical breakdown
Summary: ROS and
Cell injury
Membrane
permeability defects:
•Many causes
•Mitochondrial dysfunction
•Loss of membrane
phospholipids
•Cytoskeletal abnormalities
•ROS (reactive oxygen
species)
•Lipid breakdown products
Lysosomal
membrane injury:
•Enzyme leakage and
activation in cytoplasmÆcell
digestion: loss of glycogen,
ribonucleoprotein,
deoxyribonucleoprotein and
eventually necrosis
10
Cell Injury: reversible and irreversible
Fig. 1-13
Normal cell and the changes in reversible and irreversible cell injury. Reversible injury is characterized
by generalized swelling of the cell and its organelles, blebbing of the plasma membrane, detachment of ribosomes from the
endoplasmic reticulum, and clumping of nuclear chromatin. Transition to irreversible injury is characterized by increasing
swelling of the cell, swelling and disruption of lysosomes, presence of large amorphous densities in swollen mitochondria,
disruption of cellular membranes, and profound nuclear changes. The latter include nuclear condensation (pyknosis),
followed by fragmentation (karyorrhexis) and dissolution of the nucleus (karyolysis). Laminated structures (myelin figures)
derived from damaged membranes of organelles and the plasma membrane first appear during the reversible stage and
become more pronounced in irreversibly damaged cells. (From Kumar V, Abbas A, Fausto N: Robbins & Cotran pathologic
basis of disease, ed 7, Philadelphia, 2005, Saunders.)(McGavin, M. Donald. Pathologic Basis of Veterinary Disease, 4th
Edition. C.V. Mosby, 082006. 1.7.1.5).
Reversible Injury: Acute Cell
Swelling
• Also termed hydropic degeneration
• Most common and fundamental expression of
cell injury
• Increased cell volume and size
• Water overload due to loss of normal
homeostasis
• Major mechanisms:
– Failure of cellular injury production (hypoxia,
decreased ATP)
– Cellular membrane damage
– (injury to enzymes regulating ion channels possible
but less common)
11
Reversible Injury: Acute Cell
Swelling
• How is normal cell volume control
maintained?
• Major components are:
– Sodium/potassium ATPase pump
– Ion levels: Osmotic gradients
Ion
Inside
Outside
Na
12
146
K
Ca
– Cell membranes
K
Normal
Sodium/Potassium Pump
Ca
Na
Na
H2O
H2O
Ca
K
ATP
Mitochondria
ATP synthesis
Ion
Inside
Outside
Na
12
146
O2
Normal volume control
K
Ca
12
K
Without O2 or ATP
Sodium/Potassium Pump
Ca
Na
Na
H2O
H2O
Ca
X
K
Cell swellling
ATP
Mitochondria
ATP synthesis
X
O2
Hypoxic injury resulting in cell swelling
ATPase pumps
• ATPase pumps are often
– Slow, 10 to 103 ions/molecules/second
– All require ATP (by definition)
• ATP
ADP+Pi
ATPase
– ½ of all ATP is used to maintain transport gradients
• Types of ATPase pumps
– P, F, V, ABC
Mark Ackermann, Iowa State U. 2008
ATPase pumps
• P type ATPase pumps
– Na/K, H, Ca transported
– Locations
• Plasma membrane for Na/K
– 3 Na+ out, 2 K+ in
– For cell homeostasis
• Apical plasma membrane of stomach (H+/K+)
• Plasma membrane of all cells (Ca++)
• Sarcoplasmic reticulum (Ca++)
Mark Ackermann, Iowa State U. 2008
13
Acute Cell Swelling due to Membrane Injury
• Plasma membranes and organelle membranes
both susceptible
• Barrier failure results in influx of sodium (with
g) calcium
water following),
• Causes include modification of phospholipids by:
–
–
–
–
Free radicals
Covalent binding of toxic chemicals to macromolecules
Interference with ion channels
Insertion of transmembrane protein complexes (e.g.
complement components)
Acute Cell Swelling due to
Membrane Injury:
Carbon tetrachloride
Cell Swelling
Organelle membrane injury (e.g.
mitochondrial membranes) can lead
to cell swelling also by decreased
ATP production route.
Progression to
necrosis
Membrane Injury:
Carbon tetrachloride
Can also result in another type
of reversible injury—
Fatty Change
14
Morphology of Cell Swelling
Gross: subtle, pale, swollen, decreased specific
gravity
Hepatic swelling in a mouse exposed to chloroform 24 hours previously. The accentuated lobular pattern and
slight pallor in the liver on the left is due to acute cell swelling (hydropic degeneration) and necrosis of
centrilobular hepatocytes. The right is a normal mouse liver.
Morphology of Cell Swelling
Micro: dilution of cytoplasm, swelling of organelles, pale,
finely vacuolated
Histopathology of liver from a mouse with chloroform toxicosis. While many hepatocytes in the
centrilobular areas are necrotic, several cells at the interface of normal and necrotic are still undergoing cell
swelling (hydropic degeneration).
15
EM of Normal Cell
Morphology of Cell Swelling
EM: dilated ER, distended nuclear membrane, swollen
mitochondria, others – loss of microvilli, blebs
Morphology of Cell Swelling
EM: dilated ER, swollen mitochondria, others – myelin figure
16
Significance and Fate of Acute Cell Swelling
• Volume regulation and other functions lost
• Depends on number and type of cell affected
• Vulnerable cells include: cardiac myocytes,
proximal cortical tubules, hepatocytes,
endothelial cells, neurons and glia
• Changes are reversible if ATP/O2 restored and
membrane injury repaired
• May return to normal or near normal and may
have evidence of injury
• May reach “point of no return” and progress to
cell death.
Cell Injury and Cell Death
Cell Injury and Cell Death
•
Point of no return: not precise and likely
no single pathway
Two consistent characteristics of
irreversibility
•
1.Inability to restore mitochondrial function
2.Severe cell membrane damage
•
•
Leak of lysosomal contents leading to cytoplasmic
and nuclear degradation
Massive leak of intracellular substances and influx
of calcium
17
Cell Death:
2 main types (see lecture 2 for more)
•
Necrosis: morphologic changes (gross or microscopic) indicative of
cell death in a living animal.
Cell death following swelling (oncosis)
•
Apoptosis: a genetically determined process of cell self-destruction
that is marked by the fragmentation of nuclear DNA
DNA, is activated
either by the presence of a stimulus or by the removal of a stimulus
or suppressing agent, is a normal physiological process eliminating
DNA-damaged, superfluous, or unwanted cells (as immune cells
targeted against the self in the development of self-tolerance or
larval cells in amphibians undergoing metamorphosis), and when
halted (as by genetic mutation) may result in uncontrolled cell
growth and tumor formation called also programmed cell death
Merriam-Webster's Medical Dictionary, © 2002 Merriam-Webster, Inc
Cell death with shrinkage
Cell Death: Some new usage
• Cell death and necrosis are not synonyms
• Necrosis by oncosis and apoptosis may be
seen together
• Proposed: use necrosis for histologic
changes following cell death by either
mechanism
– Use oncotic necrosis and apoptotic necrosis
when a distinction can be and needs to be
made.
Cell death to Necrosis lag time:
Reversible changes may be visible within minutes
EM changes within 6 hrs.
LM changes occur 4-12 hrs following cell death in myocardium (irreversible injury within 20-60
minutes
Gross lesions may take 24-48 hrs unless vascular changes occur
18
Oncotic necrosis (oncosis): irreversible cell
injury by hypoxia, ischemia, membrane
damage.
• Can follow acute
cell swelling
• Final
Fi l pathways
th
involve calcium
Events in ischemia: reversible and
irreversible injury
Morphologic appearance of necrotic cells and
tissues (oncotic necrosis)
• Due to denaturation of intracellular proteins and
enzymatic digestion of the cell.
• Enzymatic digestion within the dying cells can be termed
autolysis (if after death Æ postmortem autolysis)
• Denaturation (coagulation) and protein lysis (autolysis)
are both occurring while the cells is still alive
• Denaturation begins in the live cells, contributes to cell
death, and continues in the dead cell
• Denaturation stops autolysis by inactivating enzyme and
removing their substrates, and by doing this decreases
inflammation
• It takes several hours for changes to develop
19
Ultrastructural changes
• Early stages have swelling of mitochondria with
ER dilation and fragmentation, nuclear
membrane folding, pale structureless cytoplasm,
poorly visible organelles.
• Later stage has cell collapse, shrinking,
cytoplasmic and organelle homogeneity, nuclear
density or lysis. Plasma membrane structures
(desmosomes, microvilli, cilia) distorted, lost
Electron microscopy of a renal epithelial cell that is in early oncotic necrosis. The nucleus is condensed
(pyknosis) and the mitochondria are very swollen (high amplitude).
Histologic Changes
• Nuclear. Pyknosis, karyorrhexis, karyolysis, loss.
20
Histologic Changes
• Cytoplasmic changes
– Early – pink and homogeneous by H&E
– Increasing eosinophilia with loss of ribosomal RNA
and consolidation of cytoplasmic components
– Continuing degradation results in pale, ghostlike
appearance
– Loss of adherence to basement membranes
– Rupture of loss of cell integrity – the most obvious
evidence of cell death
Renal cortex of a mouse with chloroform toxicosis. Notice that some of the epithelial cells are necrotic.
Some exhibit pyknosis while others have lost the nucleus or have a very pale nucleus (karyolysis.) Necrosis
is irreversible, but replacement cells may be regenerated depending on the tissue.
Spleen of a dog with parvovirus infection has fragmentation of lymphocyte nuclei (karyorrhexis) because of
the infection. Many of these nuclear changes may be due to apoptosis.
21
Centrilobular hepatocytes of a mouse with chloroform toxicosis are pale and necrotic. Note that in the
necrotic areas many of the nuclei are lost. This is karyolysis. Some of the dark nuclei that are remaining are
actually endothelial cell nuclei lining the sinusoids. Note the "pre-necrotic" cells that are only at the stage of
cell swelling?
Macroscopic Changes
•
•
•
•
Pale, unless blood is abundant
Soft and friable
Often sharply demarcated
May be surrounded by a red zone of hyperemia
or hemorrhage
• Because of the lag time, gross evidence of
necrosis may be inapparent in peracute of acute
death unless there are vascular changes.
Mammary gland from a cow with "peracute mastitis" due to coliform bacterial infection has large areas of
necrosis. These areas are slightly pink to gray. In the liver the necrotic areas would have been paler than
normal parenchyma but mammary gland parenchyma is very pale normally and necrotic areas are not paler.
The key to the necrosis is the line of hyperemia present here denoted as a line of demarcation.
22
Coagulation necrosis, infarcts, kidney, cow. A, Note the pale regions of acute coagulation
necrosis surrounded by a red rim of active hyperemia and inflammation (far left). B, Acute
coagulation necrosis of renal tubular epithelial cells. Necrotic cells have homogeneous
eosinophilic cytoplasm, more or less retained cell outlines, and nuclear changes such as
pyknosis and nuclear absence. H&E stain. (A, Courtesy Dr. D.E. Tyler, College of Veterinary Medicine, The
University of Georgia; and Noah's Arkive, College of Veterinary Medicine, The University of Georgia. B, Courtesy Dr. S.
Newman, College of Veterinary Medicine, University of Tennessee.) (McGavin, M. Donald. Pathologic Basis of Veterinary
Disease, 4th Edition. C.V. Mosby,
Types of Oncotic Necrosis
• Appearance depends on tissue involved, cause, and
time since injury has occurred as well as vascular
changes.
• Patterns historically derived, maybe outmoded, but still
used.
– Coagulation
g
((coagulative):
g
) denaturation develops
p and basic cell
outlines are preserved
– Caseation (caseous): implies degradation of cells into a granular
friable mass
– Liquefactive: enzyme digestion dominates, usual in CNS
– Fat necrosis: also termed enzymatic necrosis of fat
– Gangrenous necrosis: coagulation necrosis plus liquefaction and
saprophytic bacteria or mummification
Coagulation necrosis
Liver from another mouse with chloroform toxicosis has centrilobular necrosis. In this case the necrotic cells
retain their cell outlines and usually have a pyknotic nucleus. This is coagulation necrosis.
23
Coagulation necrosis
Kidney of a mouse with coagulation necrosis of some proximal tubules. The change is subtle but you can
detect the pyknotic cells and the slightly brighter homogeneous cytoplasm especially in the lower middle part
of the image.
Coagulation necrosis
Liver of a mouse with multifocal coagulation necrosis of hepatocytes. The change is easy to see here in
contrast to the more viable hepatocytes. This was due to a viral infection in this mouse. (Mouse hepatitis
virus.)
Coagulation necrosis
Heart of a dog with coagulation necrosis and mineralization. The dog had renal failure. The small blue
punctate areas in the necrotic myocytes are mineral, perhaps associated with mitochondria.
24
Coagulation necrosis
Heart of a cow showing the wall of the left ventricle with a discrete pale yellow area. There is also a pale
area associated with one of the papillary muscles. This lesion was due to embolism from endocarditis.
Caseous necrosis
These are lymph nodes from a cow with tuberculosis. The pale, friable (crumbly), pale areas are areas of
caseation (caseous) necrosis. Tuberculosis causes granulomatous inflammation and necrosis in the centers of
these granulomas. Cell walls are disrupted and tissue architecture is lost, hence caseation necrosis.
Caseous necrosis
This is histology of the lung from a cow with tuberculosis. The areas to the right are caseation (caseous)
necrosis. Cell walls are disrupted and tissue architecture is lost, hence caseation necrosis. Mineralization is
common in this type of necrosis. Tuberculosis causes granulomatous inflammation and necrosis in the
centers of these granulomas as seen in the previous image of the lymph nodes.
25
Liquefactive necrosis
Liquefactive necrosis. A, Acute polioencephalomalacia, brain, goat. A thiamine deficiency
has resulted in cerebrocortical malacia, which microscopically is liquefaction necrosis and
varying degrees of tissue separation (arrows). Scale bar = 2 cm..
(A, Courtesy Dr. R. Storts, College of Veterinary Medicine, Texas A&M University.(McGavin, M. Donald. Pathologic Basis of
Veterinary Disease, 4th Edition. C.V. Mosby,
Liquefactive necrosis
This is histology of the brain of a dog with distemper. The pale areas are zones of total cell and tissue loss, in
the CNS termed liquefactive necrosis. Grossly the areas would be fluid consistency.
This is cross section of the brain of a sheep with brain abscesses. Although abscesses are by definition an
inflammatory lesion, the neutrophils that make up the lesion lyse and also cause dissolution of parenchymal
cells leading to a watery (liquefactive) to pasty (caseous) appearance of the tissue. Abscesses with
liquefactive necrosis can occur in virtually any system, including CNS.
26
Gangrenous necrosis: dry
The blue line outlines the scrotal and inguinal area of a bull with gangrene of the skin. In this location it is
often an ischemic lesion. The tissue feels dry and leathery and is usually very dark. This is termed dry
gangrene.
Gangrenous necrosis: wet
This is udder of a sheep with a condition called "blue bag" due to Pasteurella multocida infection. The
necrosis seen here is actually wet gangrene. The surrounding tissue is well-vascularized leading to the wet
and bloody nature of it. Often saprophytic bacteria like Clostridial organisms contaminate areas of necrosis
and cause wet gangrene.
Gangrenous necrosis: dry
These are the hooves of a bovine with "fescue toxicity" a disease in which the blood supply to the distal
extremities is lost because of toxic effects on the vessels. The dry leathery appearance is termed dry
gangrene. There is still some blood in the skin indicating at least partial blood supply being present or
restored. Note that one of the claws has been lost due to the process.
27
Gangrenous necrosis: dry
Ring tail in a young rat
Fat necrosis
Three types in veterinary medicine:
•Enzymatic necrosis of fat: destruction of fat in the abdominal
cavity especially around pancreas due to leakage of activated
pancreatic lipases from fluid from injured pancreas. Lipases
split the triglyceride esters of adipocytes. Fatty acids combine
with calcium to form chalky white areas – white saponification.
•Traumatic
T
ti fat
f t necrosis:
i seen where
h
adipose
di
titissue iis crushed,
h d
such as in the pelvic canal of heifers following dystocia or
sternum of recumbent cattle.
•Abdominal fat necrosis of cattle: large masses of
retroperitoneal, mesenteric, and omental fat necrosis, cause
undetermined
Enzymatic necrosis of fat.
This cat has had previous bouts of pancreatitis. Leakage of pancreatic enzymes, such as lipase, cause
degradation and necrosis of the fat around the pancreas and in the adjacent mesentery. Necrotic fat often
becomes mineralized and so grossly the lesion is chalky to gritty as well as being pale from the mineral in the
necrotic fat.
28
Other lesions and terms traditionally used with cell death or necrosis
Zenker’s necrosis. A special antiquated term used for
coagulation necrosis of muscle.
Fibrinoid necrosis. Accumulation of pink amorphous masses
of fibrin, antibodies, and serum proteins in arterial walls due to
immune injury. Neutrophils are also often present. Also
termed fibrinoid degeneration.
Ul
Ulceration
ti and
d erosion.
i
L
Local
ld
defects
f t or excavations
ti
off the
th
surface of an organ or mucous membrane resulting from
sloughing or loss of necrotic tissue. An ulcer is full thickness
epithelial loss (through basement membrane. An erosion is
more superficial and does not penetrate basement membrane.
Zenker’s necrosis
Coagulation necrosis of muscle historically was termed Zenker's necrosis. You may see the term but the
necrosis present is no different from coagulation necrosis in other organs. The pale areas here are necrotic
and due to this cow being down for a long time. Pressure on the muscle prevents proper perfusion of the
muscle thus causing necrosis.
Zenker’s necrosis
Histology of a skeletal muscle with coagulation necrosis, historically termed Zenker's necrosis. The necrosis
present grossly or histologically is no different from coagulation necrosis in other organs. Hypereosinophilia,
cell swelling, cell shrinkage, nuclear pyknosis, and karyolysis but with retention of cell borders are all
present here and typical of coagulation necrosis.
29
Fibrinoid necrosis
Histology of a bovine with an inflammatory and necrotizing disease of the smooth muscle of the artery wall.
This type of change is termed fibrinoid necrosis. The name comes from the apparent similarity in the
appearance of the necrotic smooth muscle with fibrin. It turns out that electron microscopy has shown that
much of the pink color is indeed due to fibrin likely also with immunoglobulin and complement.
Ulcer
Esophagus from a bovine that had a partial blockage for a few days. Pressure on the foreign body from
esophageal contraction led to death of the mucosa and sloughing into the lumen leaving the raw surface. This
is an ulcer rather than an erosion in that the basement membrane has been breached and has actually exposed
underlying connective tissue and blood vessels.
Ulcers and erosions
This dog has an autoimmune disease called pemphigus vulgaris in which the cell adhesions of the epithelium
are disrupted leaving erosions and eventually ulcerations. The difference between an erosion, in which the
basement membrane is not breached, and an ulcer may be a matter of only one cell layer. Erosions often
become ulcers, as in this case.
30
Sequelae of Necrosis
• Surrounding inflammatory reaction with hyperemia and
phagocytes (necrotaxis).
• Digestion and liquefaction of necrotic debris by enzymes,
autolytic and heterolytic. Absorption of liquid debris by
lymphatics and removal of solid debris by macrophages
• Fibrosis and scarring
scarring.
• Regeneration of parenchyma if appropriate cell types
and stromal retention
• Calcification of debris slow to be removed (dystrophic
calcification)
• Formation of sequestrum
Sequestrum
The hoof and phalanges of this horse have been split down the middle. The horse had a penetrating nail
wound that fractured P3 and also allowed bacteria to enter, causing osteomyelitis. A fragment of the bone
became necrotic and isolated by the exudate and so was not resolved by normal processes. This persistent
fragment is termed a sequestrum. Sequestra happen in other tissue also, but bone is most common.
Questions for Lecture 1
About the Questions.
Cell Injury Questions from Lectures 1-4
come from two sources. Those
designated with this red # and ISU/Aub
at the bottom of the page were created
and contributed by residents and faculty
of Iowa State University and Auburn
University listed as a group. Æ
Answers for these appear at the end of
the question pages. Page numbers refer
to Pathologic Basis of Veterinary
Disease.
Other questions came from the AFIP web
site.
Questions from ISU/Aub
Contributed by:
Dr. Alicia Olivier
Dr. Katherine Gibson-Corley
Dr. Brandon Plattner
Dr. Darin Madson
Dr. Jodi Smith
Dr. Rachel Derscheid
Dr Charlie Johnson
Dr.
Dr. Molly Murphy
Dr. Tatjana Lazic
Dr. Ann Predgen
Dr. Aaron Lehmkuhl
Dr. Angela Pillatzki
Dr. Lisa Pohlman
Dr. Pete Christopherson
Dr. Brandon Brunson
Dr. Leah Ann Kuhnt
Dr. Kellye Sue Joiner
Dr. Beth Spangler
Dr. Elizabeth Whitley
31
1. Which is the best indicator of irreversible cell injury?
A. Clumping of chromatin
B. Inactivation of sodium pump
C. Severe mitochondrial damage
D. Changes in nuclear structure and function
E. Damage to the rough endoplasmic reticulum
1. C. Slauson and Cooper, p. 51 and 60 2007
10. Which of the following is not a hallmark of reversible cell injury
1. Adenosine triphosphate depletion
2. Cell swelling
3. Loss of membrane permeability
4. Severe mitochondrial damage
5. Reduced oxidative phosphorylation
A. 1
B. 1, 2
C. 3
D. 3, 4
E. 4, 5
10. D. Robbins and Cotran, p. 11
25. All of the following cytomorphologic changes characterize
irreversible cell injury EXCEPT:
1. Lysosomal swelling and disruption
2. Calcium entry into the cell
3. Acute cell swelling
4. Plasma membrane damage
5. Mitochondrial swelling with large amorphous densities
A. 1, 5
B. 2, 3
C. 3
D. 4, 5
E. 5
25. C. Pathologic Basis of Veterinary Disease, p. 10-16 2008
32
30. All of the following prevent free radical injury EXCEPT:
1. Vitamin C
2. Vitamin E
3. Superoxide dismutase
4. Sodium chloride
5. Glutathione peroxidase
A. 1, 4
B. 2, 3
C. 3
D. 4
E. 4, 5
30. D. Pathologic Basis of Veterinary Disease, p. 20 2008
35. The most common type of necrosis in the central nervous system
is:
A. Liquefactive
B. Coagulative
C. Caseous
D. Gangrene
E Apoptosis
E.
35. A. Pathologic Basis of Veterinary Disease, p. 23 2008
#18
During reperfusion injury, which processes DO NOT contribute to further
cell damage as a result of calcium (Ca++) ion influx into injured cells
(PBVD ed.4, p. 18)?
1. Direct cell membrane damage by the calcium ions
2. Lipid membrane damage due to activation of phospholipases
3 Generation of arachidonic acid
3.
4. Cytoskeleton breakdown due to protease activation
5. Chromatin degradation due to endonuclease activation
A,
B.
C.
D.
E.
1*
1, 2
1, 2, 3
2, 3, 4, 5
All of these are mechanisms of calcium-mediated damage
ISU/Aub
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#26
Which molecules are cytosolic agents involved in the neutralization
of free radicals during cell injury?
1. Vitamin C
2. Glutathione peroxidase
3. Superoxide dismutase
4. Vitamin E
5. Catalase
A. 1, 4
B. 2, 3, 4
C. 2, 3, 5
D. 1, 2, 3
E. 1, 2, 5
ISU/Aub
#29
Which of the following are cytomorphological characteristics of irreversible
cell injury? (PBVD ed.4, p. 10).
1. Plasma membrane damage
2. Mitochondrial swelling and vacuolation
3 Lysosomal swelling
3.
4. Lipid accumulation
5. Calcium entry into cell
A.
B.
C.
D.
1,2,3,4,5
1,2,4
1,2,3,5
1,3,4,5
ISU/Aub
#33
Which are common ultrastructural appearances of cell swelling?
1.
2.
3.
4
4.
5.
A.
B.
C.
D,
E.
Rarefied cytoplasm
Dilated ER, Golgi and mitochondrion
Distorted cilia, microvilli and attachment sites
Cytoplasmic precipitation of proteins and calcium
Intranuclear inclusion bodies
1, 2
1, 2, 3
1, 2, 3, 4
1, 2, 3, 4, 5
3, 4
ISU/Aub
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#35
Which of the following are cytomorphologic changes characteristic of
irreversible cell damage?
1.
2.
3
3.
4.
5.
A.
B.
C.
D.
E.
Amorphous densities in the mitochondria
Plasma membrane disruption
Mitochondrial swelling
Ca2+ entry into the cell
Lysosomal swelling
2, 4
2, 4, 5
1, 2, 4
1, 2, 4, 5
1, 2, 3, 4,5
ISU/Aub
#38
The sequence of events following exposure to carbon tetrachloride is:
1. Reduced production of apoproteins
2. Accumulation of intracytoplasmic lipid
3. Autocatalytic spreading of lipid peroxidation of microsomal
membranes
4 Peroxidation of unsaturated fatty acids of cellular membranes
4.
by the toxic metabolite
5. Production of the trichloromethyl radical by mixed function
oxidase enzymes of the hepatocyte smooth endoplasmic
reticulum
A.
B.
C.
D.
E.
5, 4, 3, 2, 1
5, 4, 3, 1, 2
4, 5, 3, 1, 2
3, 4, 1, 2, 5
4, 5, 3, 2, 1
ISU/Aub
#43
Which types of cell death is usually associated with impaired degradation
of lipids of bacterial origin?
A.
B.
C.
D
D.
E.
Liquefactive necrosis
Coagulative necrosis
Caseation necrosis
Gangrenous necrosis
Apoptosis
ISU/Aub
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#45
Increased intracellular calcium activates all EXCEPT: (PBVD ed.4, p. 18)
A. Phospholipases.
B. Endonucleases.
C ATPases
C.
ATPases.
D. Superoxide dismutases.
E. Proteases.
ISU/Aub
#54
Which are characteristic of euchromatin?
1.
2.
3.
4.
5
5.
A.
B.
C.
D.
E.
Present within the nucleus
Involved in protein synthesis
Regions of tightly coiled chromatin
Not used for the coding of amino acids
Regions of uncoiled nucleotide sequence
1, 2
1, 2, 3
1, 3, 4
1, 2, 5
2, 3, 4
ISU/Aub
#62
All of the following are major intracellular antioxidants EXCEPT: (PBVD ed.4,
p.19)
A. Glutathione peroxidase.
B. Superoxide dismutase.
C. Catalase.
D. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.
E. Ceruloplasmin.
ISU/Aub
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#65
Which are characteristic of reversible cell injury?
1.
2.
3.
4.
5
5.
A.
B.
C.
D.
E.
Swelling of endoplasmic reticulum and mitochondrion
Blebbing of cytoplasm at cell surfaces
Clumping of nuclear chromatin
Myelin figures
Lysosomal rupture
1, 2
1, 2, 3
1, 3, 4
1, 2, 5
2, 3, 4
ISU/Aub
#81
Major antioxidants include: (PBVD ed.4, p. 19)
1.
2.
3.
4.
5
5.
A.
B.
C.
D.
E.
NADPH oxidase
Cytochrome p450 oxidase
Superoxide dismutase
Catalase
Glutathione peroxidase
1,2
2,3
4,5
2,3,4
3,4,5
ISU/Aub
#99
A sequence of events from cellular injury to hydropic degeneration is:
(PBVD, p.13)
1.
2.
3.
4
4.
5.
6.
7.
Extensive vacuolation
Hypoxia
Osmotic pressure increases
Sodium and water move into cell
cell, potassium moves out of cell
ATP production decrease
More water moves into cell
Cisternae of ER distend, rupture, and form vacuoles
A. 2, 4, 5, 3, 6, 7, 1
B. 2, 5, 4, 3, 6, 7, 1
C. 4, 2, 3, 5, 6, 1, 7
D. 4, 6, 5, 3, 7, 2, 1
E ..5, 4, 3, 6, 7, 1, 2
ISU/Aub
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#109.
In fibrinoid change, the eosinophilic staining (H and E) substance
deposited in the vascular wall (tunica intima and tunica media) is
composed of which substances? (PBVD ed.4 p. 46)
1.
2.
3
3.
4.
5.
6.
A.
B.
C.
D.
E.
Immunoglobulin
Complement
Necrotic cellular debris
Mineral
Sodium urate crystals
Fibrin
1, 2
1, 2, 3
1, 2, 6
1, 2, 3, 6
1, 2, 3, 4, 6
ISU/Aub
#113
An early sequence of events following thrombosis and ischemia is (PBVD ed.4 p. 11):
1.
2.
3.
4.
5
5.
A.
B.
C.
D.
Decrease of oxidative phosphorylation in mitochondria
Decrease in ATP production
Cell swelling
4. Influx of calcium, water and sodium; concurrent efflux of potassium
Decreased sodium pump activity
1, 2, 5, 4, 3
3, 1, 5, 2, 4
4, 5, 1, 2, 3
5, 3, 4, 2, 1
ISU/Aub
#123
For each molecule of ATP used, the Na+/K+ ion pumps move:
A. 3 Na+ out of the cell and 2 K+ into the cell
B. 2 Na+ out of the cell and 3 K+ into the cell
C 3 Na+ into the cell and 2 K+ out of the cell
C.
D. 2 Na+ into the cell and 3 K+ out of the cell
ISU/Aub
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#133
Sources of cytoplasmic calcium are: (PBVD 4th ed., p. 18)
1.
2.
3.
4.
A.
B.
C.
D.
E.
Extracellular
Mitochondria
Endoplasmic reticulum
Golgi apparatus
1
2
1 and 2
1, 2, and 3
1, 2, and 4
ISU/Aub
#142
The conversion of the hydroxyl radical (.OH) to hydrogen peroxide (H2O2)
is catalyzed by: (PBVD p. 19)
A. Catalase
B. The Fenton reaction
C. Superoxide dismutase
D Glutathione reductase
E. Glutathione peroxidase
ISU/Aub
Answers to ISU/Aub questions for lecture 1.
•
•
•
•
•
•
•
•
•
•
•
•
•
18.
26.
29.
33.
35.
38.
43.
45.
54.
62.
65.
81.
99.
A
D
C
C
E
B
C
D
D
D
B
E
B
•
•
•
•
•
109.
113.
123.
133.
143.
C
A
A
D
E
ISU/Aub
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