Brock Biology of Microorganisms 11th edition ISBN 0-13-196893
Transcription
Brock Biology of Microorganisms 11th edition ISBN 0-13-196893
Brock Biology of Microorganisms 11th edition ISBN 0-13-196893-9 Prentice Hall 8th Edition: Zusammenfassung der Kapitel: http://cwx.prenhall.com/brock/ 11th Edition ?Folien? US $ 16,90: http://www.aw-bc.com/brock/ Growth of bacteria -> large surface -> high turnover Cell division Cell counting Total cell count Total cell count Viable cell count Nutrients required for growth of microorganisms • • • • • • • • Macronutriens: C/N/P/S/H/K/Mg/Ca/Na/Fe Micronutrients: Trace elements: Na Temperature pH Salt Oxygen supply Water activity (Humidity) Nutrition and Metabolism of Microorganisms Principles of Metabolism: Reduction potentials The Electron Tower NAD as a Redox Electron Carrier !freely diffusible) Used for energy generating reactions (catabolic) Nicotinamid-adenine-dinucleotide Used for biosynthetic reactions (anabolic) NAD/NADH Cycling Energy-rich compounds Energy conservation in fermentation and respiration ATP synthesized during steps in Catabolism ATP synthesized at the expense of proton motive force Glycolysis Emden Meyerhof pathway Respiration Vinegar production: Acetobacter /Gluconobacter Anoxic sewage digestion Aerobic respiration: The citric acid cycle Fatty acids Amino-acids Electron transport, proton motive force, and ATP synthesis Oxidative phosphorylation Electron transport: pH gradient and electrochemical potential = proton motive force > energized membrane > drives ATP synthesis chemiosmosis Classification in terms of energy Aerobic respiration (Chemotrophs) Glycolysis TCA cycle Ammonia oxidation Nitrosomonas Nitrite oxidation Nitrobacter Calvin cycle Chemolitotrophic metabolism Phototrophic metabolism Ribulose Bisphosphate Carboxylase Classification in terms of e-acceptor Abiotic parameters affecting growth of microorganisms Parameters that affect growth of microorganisms • • • • • • • Macronutriens: C /N /P /S/ K/Mg/Ca/Na/Fe Micronutrients: Trace elements Temperature pH Salt (Osmolarity) Oxygen supply Water activity (Humidity) Temperature classes of microorganisms ph and growth Salt and growth Hypersaline habitats and bacteriorhodopsin Oxygen and growth Generating anoxic conditions ps/pwater Microbial growth control Heat sterilization Pasteurization Ionizing radiation Filtration Chemical growth control Desinfectants The autoclave Radiation Filters Antimicrobials Measuring antimicrobial activity for external use Targets of antibiotics Commonly used antibiotics Translation Translation Cell wall synthesis Sensitivity towards antibiotics Antibiotic resistance Nosocomial Infections Appearance of multi-drug resistant pathogens * Untreatable with conv. antibiotics Antimicrobial drug-resistance Mechanisms: Drug uptake / efflux Drug modification Drug-inactivation Alteration of drug target