AMH and AFC Poster ADSA-ASAS JAM 2016 - Gobi

The repeatability of antral follicle count and anti-­‐Müllerian hormone concentration at two different postpartum stages in dairy cattle
M. Gobikrushanth1, P.A. Dutra1 , C.A. Felton2, A. Ruiz-­‐Sanchez1, T.C. Bruinjé1, M.G. Colazo2, S.T. Butler3 and D.J. Ambrose1,2
1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5, 2Livestock Research Branch, Alberta Agriculture and Forestry, Edmonton, AB, Canada T6H 5T6
# W 17841
3Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland
MATERIALS AND METHODS
INTRODUCTION
§ The determination of antral follicle count (AFC) and anti-­‐Müllerian hormone (AMH) in dairy cows and their association with fertility is an area of interest to many researchers. Day of Calving
§ Low AFC and AMH concentrations are reportedly associated with poor reproductive outcomes in dairy cattle (Mossa et al., 2012 JDS Vol 95 and Ribeiro et al., 2014 JDS Vol 97).
§ Even though, the repeatability of AFC has been reported on a specific day of different estrous cycles (Burns et al., 2005 BOR), the repeatability between random ovarian stages has not been reported.
14±1 DIM
75±1 DIM
Plasma AMH determined at a random stage of an estrous cycle
Plasma AMH determined at Day 1 of controlled estrous cycle
Figure 2: Blood samples were also collected from same 100 lactating Holstein cows to determine plasma concentration of AMH (AnshLite
Bovine AMH CLIA, Ansh Labs, Webster, TX) at 14±1 and 75±1 days in milk (DIM).
STATISTICAL ANALYSES
§ The MEANS procedure of SAS was used to obtain the mean and standard error of means for the continuous variables.
OBJECTIVE
§ The objective of this study was to evaluate the repeatability of AFC and plasma AMH concentrations at two different postpartum stages.
§ Correlations among continuous variables determined using the CORR procedure of SAS.
RESULTS
MATERIALS AND METHODS
Day of Calving
14±1 DIM
75±1 DIM
AFC determined at a random stage of an estrous cycle
AFC determined at Day 1 of controlled estrous cycle
Figure 1: The ovaries of 100 lactating Holstein cows were subjected to transrectal ultrasonography (Aloka Co Ltd., Tokyo, Japan) by a single individual using a 7.5 MHz linear array transducer to determine AFC at 14±1 and 75±1 days in milk (DIM)
Table 1: Central Statistics for AFC and AMH determined at both 14 ± 1 and 75 ± 1 DIM
AFC (#)
AFC (#)
AMH (pg/mL)
AMH (pg/mL)
14 ± 1 DIM
75 ± 1 DIM
14 ± 1 DIM
75 ± 1 DIM
(n=100)
(n=100)
(n=100)
(n=100)
Mean ± SEM
27 ± 1
23 ± 1
190.0 ± 12.8
224.8 ± 14.3
Range
10 -­‐ 53
6 -­‐ 45
13.0 – 528.8
38.2 – 774.1
37.4
42.5
66.9
63.5
CV* (%)
*CV: Coefficient of Variation The repeatability of antral follicle count and anti-­‐Müllerian hormone concentration at two different postpartum stages in dairy cattle
M. Gobikrushanth1, P.A. Dutra1, C.A. Felton2, A. Ruiz-­‐Sanchez1, T.C. Bruinjé1, M.G. Colazo2, S.T. Butler3 and D.J. Ambrose1,2
1Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB, Canada T6G 2P5, 2Livestock Research Branch, Alberta Agriculture and Forestry, Edmonton, AB, Canada T6H 5T6
# W 17841
3Teagasc, Animal & Grassland Research and Innovation Centre, Moorepark, Fermoy, Co. Cork, Ireland
RESULTS CONCLUSIONS
§ Results indicate that both AFC and AMH concentrations are correlated at different stages postpartum, although the correlation is stronger for AMH.
§ In summary, we conclude that both AFC and AMH are repeatable at different postpartum stages in dairy cows.
ACKNOWLEDGEMENTS
Figure 3: Correlation between AFC at 14 and 75 DIM (r = 0.41; P < 0.01)
Figure 4: Correlation between plasma AMH concentration at 14 and 75 DIM (r = 0.75; P < 0.01)
CONTACTS
§ Gobi – gobikrus@ualberta.ca
§ Divakar Ambrose – divakar.ambrose@gov.ab.ca
Figure 5: Correlation between AFC and plasma AMH conc. at 14 DIM (r = 0.57; P < 0.01)
Figure 6: Correlation between AFC and plasma AMH conc. at 75 DIM (r = 0.59; P < 0.01)