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ITALIAN MEDITERRANEAN WATER BUFFALO The Best Genetics Available in Italy EEC code IT01 bu ARTIFICIAL INSEMINATION CENTER O-B-ONE da Izano MALANDRINO III Sire TEOSIO IT003900021247 Pedigree Index PKM 108,5 IT019500215620 VG 89 PIA IT003900001886 Milk 485,5 F. 33,5 P. 20,3 2 03.06 214 2805 7,9 4,6 MALANDRINO III Dam VG 89 TERZA IT003900021248 n° year days Milk F% P% OTTAVIA IT003108000842 1 02.07 270 4623 7,2 4,3 2 04.09 270 6460 8,4 4,5 n° year days Milk F% P% 2 03.11 270 4069 7,4 4,4 Foto 20-09-2006 Daug. 82 Daug. 112 Lact. 156 Herds 36 96% R 116 PKM 119 +221,30 +969,86 +64,97 -0,46 +42,63 -010, 116 Milk 111 123 F Kg 124 123 F% 120 115 130 P Kg 94 104 P% 138 88 100 112 124 136 148 Data ANASB Sept. 2014 Udder close up T059990136387 ZERBIO’s Daughter Owner: Caprarelli F.lli Zerbio’s daughters distinguish themselves as animals with a big structure and for the excellent udder composite OTTAVIA – ZERBIO’s Maternal Granddam TERZA - ZERBIO’s Dam Data ANASB June 2014 Herds 28 116 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength Front Udder Attachment Rear Udder Attachment Distance to the Heals Teats Direction Teats Size Sire O-B-ONE da Izano ALDESTE SA 42196 VG 89 JAFAR IT054CR003C167 PKM 45,70 Data ANASB 2014 IT019500215620 INSEGNA CR 630 G+ 82 6 2001 270 4161 8,0 4,6 ALESSIO CR 721 Dam RESICA CR 1574 VG 89 VG 86 n° year days Milk F% P% Az 35 CE 24597 G+ 81 1 2 3 4 5 Foto 20-09-2006 Available Straws Daug. 273 Herds 48 270 270 270 270 270 3237 5178 5118 4510 5027 8,0 7,4 7,6 7,9 7,8 4,5 4,2 4,3 4,6 4,4 n° year days Milk F% P% 6 1998 270 4379 7,2 4,0 95% R Daug. 381 Lact. 833 Herds 58 132 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength Front Udder Attachment Rear Udder Attachment Distance to the Heals Teats Direction Teats Size Data ANASB June 2014 0,50cc and 0,25cc 1998 2000 2001 2002 2004 99% R 131 PKM 97 +32,00 +154,26 +10,81 -0,06 +5,64 -0,06 135 Milk 134 122 F Kg 125 128 F% 101 126 116 P Kg 105 123 88 100 112 124 P% 136 148 Data ANASB Sept. 2014 RESICA - Dam of O-B-ONE Owner: Massari F.lli RESICA owner: Massari F.lli O-B-ONE daughter Reserve Champion at the interregional fair in Eboli Salerno 2007 Sire TONINO II° CE 24521 VG 85 NAPOLI CE 28216 PKM 4,49 Data ANASB 2012 NONTISAZI MAI CE 18428 4 1998 270 2884 8,1 MALANDRINO CE 24552 Dam INSEGNA CR 630 G+ 82 n° year days Milk F% P% COLORIZIO CE 28255 Daug. 154 Herds 38 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength Front Udder Attachment Rear Udder Attachment Distance to the Heals Teats Direction Teats Size Data ANASB June 2014 0,50cc and 0,25cc 270 270 263 270 270 270 2713 3726 3664 3944 3849 4161 9,7 8,0 7,7 8,1 7,8 8,0 4,7 4,5 4,7 4,7 4,6 4,6 n° year days Milk F% P% 6 1998 270 4379 7,2 4,0 Daug. 217 Lact. 546 Herds 44 110 110 PKM 117 111 109 F Kg 115 102 105 F% 114 118 P Kg 104 109 P% 98 88 100 112 124 136 148 98% R +56,90 +225,76 +17,09 -0,04 +10,71 +0,01 Milk 100 JAFAR fratello materno di CAESAR JAFAR Owner: Massari F.lli 93% R 1995 1996 1997 1999 2000 2001 Data ANASB Sept. 2014 INSEGNA – CAESAR’s Dam INSEGNA Owner: Massari F.lli Available Straws 1 2 3 4 5 6 Sire VG 85,5 MALANDRINO CE 24552 MASTER IT054CR003C202 CR 1595 MALANDRINO CE 24552 Dam INSEGNA CR 630 G+ 82 n° year days Milk F% P% COLORIZIO CE 28255 1 2 3 4 5 6 Foto 20-09-2006 Daug. 123 Herds 39 270 270 263 270 270 270 PKM 126 124 Milk 118 118 107 F Kg 124 111 F% 125 126 P Kg 92 101 P% 98 100 9,7 4,7 8,0 4,5 7,7 4,7 8,1 4,7 7,8 4,6 8,0 4,6 n° year days Milk F% P% 6 1998 270 4379 7,2 4,0 Daug. 177 Lact. 378 Herds 51 124 88 2713 3726 3664 3944 3849 4161 88% R 124 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength Front Udder Attachment Rear Udder Attachment Distance to the Heals Teats Direction Teats Size 1995 1996 1997 1999 2000 2001 112 124 Data ANASB June 2014 136 148 98% R +92,60 +411,86 +28,30 -0,18 +17,38 -0,07 Data ANASB Sept. 2014 JAFAR fratello materno di CAESAR e LUCKY JAFAR Owner: Massari F.lli INSEGNA Owner: Massari F.lli INSEGNA – LUCKY’s Dam T E S T E D Pictured Foto 10-05-2005 10-05-2005 Born 16 Jan, 2007 IT003990028287 Owner: Facchi Flli Oleggio Novara Italy Daug. 60 Lact. 83 Herds 22 93% R B U L L S Born 20 June 2003 IT061000104834 Owner: Agricola Vitale Ciorlano Caserta Italy Daug. 70 Lact. 152 Herds 22 PKM +10,20 Milk +224,86 +1,81 F Kg -2,42 F% +0,25 F% -0,86 P Kg -0,39 P Kg +4,38 +0,13 P% PKM +1,60 Milk -66,54 F Kg P% -0,26 Data ANASB Sept. 2014 Data ANASB Sept. 2014 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength EX 90 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength 101 101 101 101 101 101 88 Sire 95% R 100 112 124 136 101 101 101 101 101 101 88 148 MILLENNIUM SA 61796 Sire 100 112 124 136 148 VG 86,5 IT061000008273 CIRIPICCHIO IT065800814207 PKM 10,20 Data ANASB 2014 OLMO SA 49041 G+ 83 6 07.10 252 5963 9,1 4,4 RADAR SA 49426 Dam IT021AP357C126 G+ 84 Dam LINUS VA 1008 TAFANA IT003900021231 n° year days Milk F% P% FARFALLA VA 743 G+ 81 1 02.04 270 3936 6,7 4,2 2 04.07 270 4433 7,3 4,5 n° year days Milk F% P% 5 07.04 270 3401 8,2 4,2 n° year days Milk F% P% GINESTRA AP 0000009 1 2 3 4 5 6 03.01 04.07 05.08 06.09 07.10 08.11 270 252 270 245 270 270 3909 3913 3975 3473 4479 4026 6,0 4,0 8,7 4,6 7,7 4,4 7,1 4,4 7,4 4,2 9,3 4,7 n° year days Milk F% P% 4 05.10 270 2187 8,4 4,5 BLACKSTAR #1 of the YOUNG BULLS The best bull in A.I. with a Sire & a Maternal Grandsire indexed in Production & Morphology Sire VG 89 Malandrino ITCE000024552 VG 89 Malandrino III IT054CR003C174 PKM 177,30 Data ANASB 2014 Resica ITCR000001574 VG 86 3 05.01 270 5118 7,6 4,3 Dam PKM 175,10 Sept. 2014 Lucky IT019500348511 VG 87 PKM 92,60 Data ANASB 2014 River Perla IT077990042258 G+83 Foto 20-09-2006 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength Front Udder Attachment Rear Udder Attachment Distance to the Heals Teats Direction Teats Size n° year days Milk F% P% Az 3002 IT059990044055 G+ 84 1 02.07 270 4465 7,4 4,7 2 03.10 270 4285 8,0 4,8 n° year days Milk F% P% 3 04.10 270 4712 7,0 4,4 117,5 118,5 112,5 116 +111,80 Milk +504,50 F Kg +34,73 F% 117 123 -0,22 P Kg 102,5 +20,94 P% 124 -0,09 108,5 119,5 124 105 104 100 112 124 136 148 Data ANASB June 2014 Blackstar has a fantastic Projected Morphology Index, as a matter of fact as you may see from the graph the bulls has a strong positive impact on all of the principal trait. He is especially good on the Front and Rear Udder Attachment, exceptionally strong on Structure, Stature and Body Length. He is very strong also on the Feet & Legs Index and Pasture, characters that are very important on the buffalo selectin. Blackstar for sure is a good promise in improving the production and on all of the morphological trait. PKM +176,20 Milk +819,41 F Kg +49,21 F% -0,35 P Kg +34,95 P% -0,09 Data ANASB Sept. 2014 LUCKY Owner: Massari F.lli 101 88 MALANDRINI III° Owner: Massari F.lli PKM Sire VG 85 JAFAR IT054CR003C167 O-B-ONE IT019500215620 PKM 32,00 Data ANASB 2014 RESICA CR 1574 VG 86 3 05.01 270 5118 7,6 4,3 IT019500131505 Dam FAEMA IT019500263675 n° year days Milk F% P% 1 2 3 4 JAFAR 02.02 03.04 04.05 05.05 270 242 260 270 3185 3461 3839 3410 7,2 4,4 8,0 4,5 6,6 4,4 8,6 4,5 IT051CR035C058 G+ 84 n° year days Milk F% P% 4 05.07 270 3670 7,5 4,4 da Izano IT054CR003C167 Sire VG 85 JAFAR IT054CR003C167 O-B-ONE IT019500215620 PKM 32,00 Data ANASB 2014 RESICA CR 1574 VG 86 3 05.01 270 5118 7,6 4,3 LINUS VA 1008 Dam TAITI IT003900021197 n° year days Milk F% P% 1 2 3 4 02.01 03.07 05.00 06.03 270 270 270 270 Sire 3809 4940 4535 4303 6,9 4,5 7,9 4,6 7,9 4,7 7,2 4,2 G+ 84 n° year days Milk F% P% LT 6997 EX 93 LT 6710 G+ 84 CIRCE 1 IT059000066852 PKM 13,80 Data ANASB 2014 4 05.11 270 3994 8,6 4,7 IT051CR035C004 VG 88 Dam COROLLA IT019500176494 Pictured 20-09-2006 n° year days Milk F% P% 1 2 3 4 5 02.06 03.09 05.01 06.04 07.06 270 270 260 270 270 2317 3205 3162 3236 3301 9,0 4,8 8,3 4,5 7,8 4,4 8,4 4,4 8,6 4,4 CR 1469 G+ 80 n° year days Milk F% P% 8 10.01 270 3382 9,2 4,9 CICLOPE a buffalo with a Sire and both Grandsire with an Index in Production and Morphology Sire VG 85 Jafar IT054CR003C167 VG 89 PKM 45,70 Data ANASB 2014 O-B One IT019500215620 PKM 32,00 Data ANASB 2014 Resica ITCR000001574 VG 86 3 05.01 270 5118 7,6 4,3 Dam PKM 264,40 Sept. 2014 Malandrino III IT054CR003C174 PKM 177,30 Data ANASB 2014 UDEA IT003990003934 VG 86 Foto 20-09-2006 General Score Structure Feet & Legs Dairiness Mammary System Stature Body Depths Body Lengths Feet Strength Front Udder Attachment Rear Udder Attachment Distance to the Heals Teats Direction Teats Size n° year days Milk F% P% OLIMPIA IT003108000786 G+ 80 1 2 3 4 n° year days Milk F% P% 1 02.04 270 3959 8,7 4,8 270 270 270 270 4880 5094 4637 4627 7,8 7,2 8,2 8,2 4,8 4,6 4,4 4,4 121,5 122 98 121,5 125 125 PKM +60,33 Milk +277,90 F Kg +19,23 F% 102,5 126 -0,12 P Kg 103,5 120 +11,12 P% -0,07 PKM +148,20 Milk +688,86 F Kg +40,84 108 105 115,5 94 100 112 124 136 148 CICLOPE is a progeny buffalo waiting to be indexed that has excellent positive indexes, in particular the Projected Morphology Index; as you can notice it shows a good structure, fantastic udder and exceptional teats size and position, farther more the daughters are animal wellstructured with great milk characteristics. A sure promise for the Italian buffalo selection. F% -0,24 P Kg +29,54 P% -0,04 Data ANASB Sept, 2014 MALANDRINO III° Owner: Massari F.lli 88 Data ANASB June 2014 O-B- One Owner: Massari F.lli 02.03 03.10 05.01 06.06 Sire VG 85 NAPOLI CE 28216 CAESAR IT019500148631 PKM 4,49 Milk +20.03 PKM 56,90 Data ANASB 2014 INSEGNA CR 630 G+ 82 6 08.02 270 4161 8,0 4,6 Dam PKM 153,10 Sept. 2014 IT051CR035C004 VG 88,5 FIULA IT019990236392 n° year days Milk F% P% Foto 22-10-2009 1 02.07 270 3010 8,0 4,3 2 04.05 270 3833 7,6 5,0 3 05.09 270 4515 7,8 5,1 BAGOLA IT019500131508 n° year days Milk F% P% 3 05.02 258 2596 7,9 4,9 CAESAR Milk +462,26 F Kg +24,51 F % -0,31 P Kg +22,57 P % +0,06 Milk F Kg P Kg +698,76 +31,92 F % -0,58 +34,42 P % +0,11 Data ANASB Sept. 2014 Data ANASB Sept. 2012 PASSATORE Extraordinary son of Malandrino III° waiting to be indexed Sire IT054CR003C174 MALANDRINO CE 24552 Malandrino III IT054CR003C174 PKM 177,30 Data ANASB 2014 RESICA CR 1574 VG 86 3 04.06 270 5118 7,6 4,3 Dam PKM 169,80 Sept.2014 VG 84 AZ 3/95 ITLT000010377 EX 90,5 Az. 1063 IT059990018965 MALANDRINO III° Milk +777,61 F Kg +52,29 F % -0,24 P Kg +32,89 P % -0,11 PASSATORE’s Sire’ Data ANASB Sept, 2014 n° year days Milk F% P% AZ 336 ITLT000008380 G+ 83 1 2 3 4 5 n° year days Milk F% P% 3 05.03 270 4329 8,0 4,5 02.07 03.08 04.07 05.08 06.10 Milk F Kg P Kg 270 268 241 270 270 3039 3655 3208 4173 3977 8,3 4,5 8,6 4,6 8,1 4,5 7,6 4,4 7,9 4,6 +752,16 +49,28 F % -0,13 +32,59 P % -0,06 Data ANASB Sept, 2014 RAINBOW a buffalo that is very promissing in milk production and has exceptional deviation in Protein Sire VG 85 ITCE000055811 Jesce Sole IT061000057361 PKM 121,30 Data ANASB 2014 IT061000023393 G+ 80 5 08.06 270 3566 9,6 4,9 Dam PKM 252,20 Sept, 2014 River Alida IT059990056213 G+ 84 n° year days Milk F% P% Foto 20-09-2006 PKM 252,20 Milk +751,36 F Kg +61,01 F% +0,00 P Kg +52,18 P% +0,50 River ALIDA is the # 1 for % of Protein ALIDA is a fantastic buffalo of the Bradano River herd located in Matera, it has beside an extraordinary milk production and exceptional percentage of Fat and Protein. ALIDA is the best buffalo in Italy in the Protein percentage Index with a +0.50%. % of Protein PKM Milk F Kg F% P Kg P% Data ANASB Sept, 2014 6,20 6,10 6,00 5,90 5,80 5,70 5,60 5,50 5,40 5,30 5,20 IT059990000817 1 02.06 270 2722 9,9 5,7 2 03.09 270 4062 9,1 5,5 3 04.11 270 4858 8,6 5,7 1° Lactation 2° Lactation 3° Lactation PERCENTAGE OF PROTEIN PRODUCTION IN 3 DIFFERENT LACTATIONS PKM +186,75 Milk +667,86 F Kg +47,14 F% -0,08 P Kg +38,30 P% +0,19 Data ANASB Sept, 2014 30 60 90 120 150 180 210 240 270 25,0 MILK PRODUCTION IN 3 DIFFERENT LACTATIONS Daily Milk Production in Liters 22,5 1° Lactation 2° Lactation 3° Lactation 20,0 17,5 15,0 12,5 10,0 7,5 5,0 2,5 0,0 30 60 90 120 150 Days of Lactation 180 210 240 270 JESCE SOLE Owner: Garofalo F.lli Caserta Days of Lactation Sire 11/96 ITLT000013046 VG 88,5 7/06 IT059990064965 VG 86 AZ 613 IT059000058777 G+ 84 4 Dam PKM 184,20 Sept. 2014 05.11 237 3405 8,1 4,6 3/95 ITLT000010377 EX 90,5 AZ 1169 IT059990037772 G+ 80 JAFAR n° year days Milk F% P% AZ 345 IT059000024181 G+ 82 1 2 3 4 5 8,9 7,9 7,0 7,7 8,3 n° year days Milk 5 07.07 270 3101 02.10 04.00 05.02 06.02 07.04 270 270 229 270 270 3866 3742 3713 3847 4117 4,6 4,7 4,3 4,5 4,4 F% P% 8,9 4,6 da Izano IT054CR003C167 Sire VG 85 ITCE000055811 JESCE SOLE IT061000057361 PKM 121,3 Milk 584,36 Sept. 2014 Dam PKM 157,10 Sept. 2014 IT061000023393 G+ 80 5 08.06 270 3566 9,6 4,9 ITLT000011712 VG 89,5 AZ 1002 IT059000237648 G+ 83 n° year days Milk F% P% AZ 898 IT059000107895 G+ 81 1 2 3 4 5 8,4 9,0 8,4 7,0 7,7 n° year days Milk 2 04.01 250 2470 02.11 04.05 05.05 06.06 07.10 270 237 230 270 270 Sire 3829 3907 3666 3532 3485 4,6 4,7 4,6 4,4 4,4 VG 85 F% 8,7 P% 4,7 ITCE000055811 JESCE SOLE IT061000057361 PKM 121,3 Milk 584,36 Sept. 2014 Dam PKM 137,00 Sept. 2014 IT061000023393 G+ 80 5 08.06 270 3566 9,6 4,9 ITLT000006997 EX 93 AZ 935 IT059000107917 n° year days Milk 1 2 3 4 5 6 7 02.05 03.08 04.10 05.10 06.10 07.10 09.01 270 270 266 270 245 270 270 3071 3810 3380 3564 2999 2814 3534 F% P% 8,0 7,4 8,5 8,4 8,1 7,7 8,1 4,6 4,7 4,7 4,7 4,6 4,7 4,7 AZ 119 ITLT000006715 G+ 80 n° year days Milk F% P% 4 06.00 270 4267 8,1 4,6 Prosynch protocol in buffalo heifers Diagram of ovarian dynamics and synchronization response for Prosynch Protocol hormone injection schedule in Buffaloes heifers Pic. 1 7 days F CL Regression Ovulation or CL formation 48 hours 16 hours CL CL Ovulation or CL formation A.I. F 8 days GnRH CL PGF 2cc GnRH 3 days GnRH PGF 2cc 0 days In order to obtain great result in conception rate, you don’t only need good herd management with a correct ration and a good technic in artificial insemination, you also need to have great quality semen material. That is why COFA, on this matter has always put special attention, and has come up with specific protocol for the semen buffalo production. At COFA, we don’t only look at the motility and concentration of the semen, but we pay very good attention at the morphology evaluation of the sperm, that allows us to make and estimate of the fertility, not only in theory at the lab but also on hand in the field. CL CL Regression CL from earlier GnRH Plus CL formed from this GnRH Prosynch pic. 1 Is the appropriate protocol for synchronization of the ovulation for the buffalo heifers Ovsynch protocol in buffalo Diagram of ovarian dynamics and synchronization response for traditional OvSynch hormone injection schedule F=follicle; CL=corpus luteum Pic. 2 7 days CL 48 hours A.I. GnRH PGF 2cc GnRH 0 days F 28 ± 4h ovulation Another very important aspect, in order to be able to produce high semen quality, is the freezing, a very delicate process. That is why over the years we have created and perfected a SPECIAL curve only for the buffalo semen, with great result. 16 hours CL Day 0: 1 shot of GnRH Regression Day 7: 1 shot of prostaglandine F2a Ovulation Day 9: 1 shot of GnRH 48 hours after PGF2a or CL formation Day 10: Inseminate 16 hours after day 9 GnRH shot Ovsynch pic. 2 Is the appropriate protocol for ovulation induction for the buffalos in lactation. Resynch protocol in buffalo Pic. 3 TAI day 0 PGF2a to open buffaloes GnRH + TAI (16 h) later GnRH to all Buffaloes day 28 day 35 day 37 Ultrasaund Buffaloes closed Resynch pic. 3 ( INSEMINATION PRESSING ) Is the appropriate protocol in order to reduce the synchronization times of the unpregment buffalos and to strengthen the pregnant buffalos. During the year 2014 we had 14 buffalo in production, and we had an excellent result on the quality of the semen on all of them like it shown on the table below: PROGRESSIVE MOTILITY 57,0% TOTAL CONCENTRATION 18,1 millions NPMS (Number of Progressive Motile Sperm) 10,3 millions MORFOLOGY anomalies less than 20% With this result COFA puts itself as the world leading company in the production of high quality buffalo semen material. In the semen we make, you won’t only find and get high genetics, but you’ll also get great sperm quality. We can say this because of the feedback we get from or numerous client and veterinarian that operates in the field in Italy and all over the world. Cinzia Pozzaglio COFA Laboratory Technician Az. Ag. Facchi f.lli – Heifers Barn Via Orezola snc 26048 Sospiro Cremona Italy Warehouse of the A.I. Stud Benedetta Bischeri Ph. : (+39) 0372.621248 Fax: (+39) 0372.621519 e-mail: recapito@cofa-it.com Web site: www.cofa-it.com Laboratory of the A.I. Stud Cinzia Pozzaglio Ph. : (+39) 0372.621248 Fax: (+39) 0372.621519 e-mail: lab@cofa-it.com Foreign Market Mattia Casali e-mail: mattia@cofa-it.com Skype: mattia1465 Cell.: (+39) 335.6155915 Accounting Maddalena Maiocchi e-mail: m.maiocchi@cofa-it.com Ph.: (+39) 0372.28842 Fax: (+39) 0372.26106 Italian Market Giovanni Binotti G.B. GENETICS e-mail: giovanni.cofa@libero.it Cell.: (+39) 338.5827257 Bradano River ZEUDI IT059990072579 2° lact. 299 days Milk Kg 4.727 F % 8,42 P % 4,96 BEST UDDER at the National Fair in Foggia in 2010