ACUSYST-XCELLERATOR

ACUSYST-XCELLERATOR™
ACUSYST-XCELLERATOR™
Flexible, pilot-scale bioreactor:
- 500L fed-batch bioreactor equivalent… up to …
- 1650L fed-batch bioreactor equivalent
- Various sizes™of disposables provide flexibility
Linear scale-up from Biovest’s benchtop systems
Used to produce an FDA-licensed injectable
Process-development,
pilot-scale
bioreactor
Diagnostic
and therapeutic
uses
80L fed-batch
bioreactor
equivalent
Produce MAbs, recombinant proteins or virus
Diagnostic and therapeutic uses
Culture suspension or adherent cell lines
Produce MAbs, recombinant proteins or virus
Simultaneously produce 1 or 2 products
ACUSYST-miniMAX
ACUSYST-
miniMAX ™
Culture suspension or adherent cell lines
Cell Culture System
Single-use disposable:
Single-use disposables:
• Supplied sterile and ready-to-use
validation
- Supplied
sterile and
• No cleaning
or expense
• Fast turnaround time
ready-to-use
- No cleaning validation or expense
In use worldwide for many years producing
- Fast
time
regulated biologics
with turnaround
very high reliability!
2 gm/day from routine cell
Produces upFast
to ~1/acquisition
cycle… <4
lines
months from PO to completion of IQ/OQ and operator training
Runs routinely last for months—maximizing
your production uptime
Hollow Fiber Advantages
Protein Production and
Concentration in the
Bioreactor!
Mother Nature knows this already! Capillaries efficiently
perfuse dense tissues. Hollow fiber capillaries are
Hollow
Fiber Advantages
semipermeable
membranes (<60KDa MWCO). Nutrients
Mother
Nature
knows
already!
and
wastes
freelythis
exchange.
Cells, growth supplements
Capillaries efficiently perfuse dense tissues.
andfiber
products
are retained.
Products are simultaneously
Hollow
capillaries
are semipermeable membranes (10KDa MWCO).
Lactate
cross
CROSS
section
SECTION
produced and concentrated! Growth supplements are
Nutrients and wastes freely exchange.
needed in very small amounts to save $$$ and create purer
Glucose
Glutamine
O2
CO2
NH4
Cells, growth supplements and
products
are retained.
supernatant
than in other culture methods.
Products are simultaneously produced
and concentrated!
Growth supplements are needed in very
small amounts to save $$$ and create purer
supernatant than in other culture methods.
Creating the next generation of therapeutic and diagnostic products
Protein Production and
Concentration in the Bioreactor!
Biovest.com
ACUSYST-XCELLERATOR™
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Additional Features
Save Time & Money
XCELLERATOR automatically controls:
XCELLERATOR uses two feed media:
•
•
•
•
•
• e.g., DMEM:F12 (no supplement)
• e.g., DMEM:F12 with 5% FBS or
~125 to
~2 to
Various commercial
serum-free
250L /
4L / media
month
Use very littlemonth
growth supplement!
pH
Incubator temperature
~125L /
~2L /
Refrigerator temperature
month
month
EC Cycling (tissue perfusion rate)
Continuous or batch harvesting
~750 to
2500L /
month
~12 to
40L /
month
XCELLERATOR produces concentrated and
optionally clarified supernatant. Go
directly from bioreactor to purification!
Oxygenator
~ 5L /
week
Inside CO2 Incubator
Hollow Fiber Means
High Cell Density!
Oxygenator
~4x1012 viable cells uniformly
distributed throughout the bioreactor.
Specifications &
Requirements
418 kg
18–26°C ambient
100% CO2, 14–16 psi
134 cm W x 85 cm D x 203 cm H
~
100–230 VAC,
Hz, ~3000 watts, 30L50/60
/ week
Single-phase
XCELLERATOR
Product
1650L Fed-Batch Reactor
FACILITY
Compact & Rolls!
100% CO2,
Standard electricity
Large skid, multiple
gases, CIP, SIP, etc.
Complexity!
SEED TRAIN
4L Inoculum
week
Simple static
culture methods
80L Inoculum
Complex, Expensive,
Time-consuming
MEDIA COSTS
EXAMPLE
$66/gm
MAb produced
$300/gm
MAb produced
DOWNSTREAM
PROCESS
260L of Supernatant
Supernatant is ready
for purification!
1600L of Supernatant
Concentration,
Clarification…
Expense and time
TURNAROUND
EXPENSE
Runs last months
Maximizes uptime
Disposable = Rapid
turnaround
10-Day Runs.
Frequent cleaning and
startup costs.
Lower productivity
~ 0.15 L /
Fresh
Product
Support
You focus on the science. We’ll focus on providing robust, easy-to-operate,
and low-maintenance bioreactors. Our bioreactors are successfully operated
by technicians with routine cell culture experience, not chemical engineers.
~0.15
Our technical support assists customers
withL achieving
their success. Fresh
/ week / bioreactor
Operates inside CO2 incubator
Biovest provides training, IQ/OQ, field service, and preventive maintenance
for its bioeactors.
Curt Gleiter, Client Service - Instruments
8500 Evergreen Blvd. | Minneapolis, Minnesota 55433 USA
(763) 786-0302 ext. 215 | cgleiter@biovest.com