Immunodeficient Model Xenograft Data

Transcription

Technical Book
Charles River Immunodeficient Models
Xenograft Data Catalog
Volume 1
askcharlesriver@crl.com
www.criver.com
© 2015, Charles River Laboratories International, Inc.
Technical Book
Table of Contents
1 Oncology Animal Models
Nude Model
2 Athymic Nude Mouse
Severe Combined Immunodeficiency (SCID) Models
2 Fox Chase SCID® Mouse (C.B-17 SCID)
2 Fox Chase SCID® Beige Mouse
3-24 Human Tumor Xenografts
Bladder
3 SW780 tumor growth graph
Brain
3 U251 tumor growth graph
3 U-87 MG tumor growth graph
Breast
4 BT474 tumor growth graph
4 HCC1954 tumor growth graph
4 JIMT-1 tumor growth graph
4 MCF-7 tumor growth graph
5 MDA-MB231 tumor growth graph
5 MX-1 tumor growth graph
Colon
5 COLO205 tumor growth graph
5 HCT116 tumor growth graph
6 HT29 tumor growth graph
6 SW620 tumor growth graph
Epithelial
6 A431 tumor growth graph
Gastric
7 MKN-45 tumor growth graph
7 NCI-N87 tumor growth graph
7 SNU5 tumor growth graph
Head and Neck
7 FaDu tumor growth graph
Leukemia
8 HL-60 tumor growth graph
8 K562 tumor growth graph
8 MOLM-13 tumor growth graph
8 MOLT-4 tumor growth graph
9 MV4-11 tumor growth graph
9 SET2 tumor growth graph
Liver
9 SNU-398 tumor growth graph
9 Hep3B tumor growth graph
Lung (non-small cell)
10 H226 tumor growth graph
13 HCC-44 tumor growth graph
13 NCI H2122 tumor growth graph
13 SKMES tumor growth graph
Immunodeficient Models Xenograft Data Catalog - Volume 1 Lung (small cell)
15 SHP-77 tumor growth graph
15 DMS-114 tumor growth graph
Lymphoma
16 Daudi tumor growth graph
16 DOHH-2 tumor growth graph
16 Granta-519 tumor growth graph
16 Ramos tumor growth graph
17 RL tumor growth graph
17 SU-DHL-4 tumor growth graph
17 SU-DHL-6 tumor growth graph
Melanoma
18 SKMEL-5 tumor growth graph
Mesothelioma
18 MSTO-211H tumor growth graph
Multiple Myeloma
19 OPM-2 tumor growth graph
19 RPMI-8226 tumor growth graph
Ovarian
20 SKOV3 tumor growth graph
20 OVCAR3 tumor growth graph
20 TOV-21G tumor growth graph
Pancreas
21 Bx-PC3 tumor growth graph
21 Capan-1 tumor growth graph
21 MiaPaCa-2 tumor growth graph
21 PANC1 tumor growth graph
Prostate
22 CWR22Rv1 tumor growth graph
22 PC3 tumor growth graph
Renal
22 786-O tumor growth graph
Sarcoma
23 HT1080 tumor growth graph
23 SJSA-1 tumor growth graph
Thyroid
23 8505c tumor growth graph
23 FTC-238 tumor growth graph
Uterine
24 MFE-280 tumor growth graph
25-26 Cell Line Testing - 360 Diagnostics
25 Rodent CLEAR Panels
26 Human CLEAR Panels
26 Contamination CLEAR
27 Discovery Services
27 Collection of Oncology Research Experiments (CORE)
27 Supplemental Services
Research Models
Oncology Animal Models
Overview
Oncology is one of the leading areas of research into new therapeutics. Due to the challenges inherent in researching and developing
anticancer therapeutics, it is critical that you have the right tools and resources available to you. Backed by decades of technical, scientific
and veterinary experience, Charles River’s global portfolio of high-quality oncology models gives you the benefit of partnering with an
industry leader offering an infrastructure capable of advancing your research now and in the future. The following information provides an
overview of Charles River’s portfolio of immunodeficient oncology animal models produced in North America.
Immunodeficient Animal Models
Immunodeficient animal models are extremely useful in a wide range of biomedical research, including infectious disease, stem cell,
immunology and oncology studies. Due to the unique vulnerability that makes these models vital to research, their care and maintenance
demands a high level of expertise and technological resources.
Selecting the most appropriate animal model to use is one of the more challenging steps a researcher must take when designing an
oncology study. Quite often, resources and time are logged performing literature searches for what has been previously published or
conducting trial and error pilot studies to assess positive tumor growth. As such, Charles River has developed the following information in
an effort to assist you in expediting your model selection process.
The following table illustrates the Charles River portfolio of immunodeficient animal models. Depending on your research design and cell
line, it is critical to understand the level of immunodeficiency each model possesses.
Strain
Hair
T-Cell Deficient
B-Cell Deficient
NK Cell Deficient
Athymic Nude Mouse
No
Yes
No
No
CD-1 Nude Mouse
No
Yes
No
No
NU/NU Mouse
No
Yes
No
No
BALB/c Nude Mouse
No
Yes
No
No
NIH-III Mouse
No
Yes
Yes
Impaired
No
Yes
No
No
No
Yes
Yes
No
SCID Hairless Congenic (SHC™) Mouse
No
Yes
Yes
No
Fox Chase SCID® Mouse
Yes
Yes
Yes
No
Fox Chase SCID® Beige Mouse
Yes
Yes
Yes
Impaired
NOD SCID Mouse
Yes
Yes
Yes
Impaired
®
RNU Rat
SCID Hairless Outbred (SHO ) Mouse
®
Research Models
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Immunodeficient Models Xenograft Data Catalog - Volume 1
Technical Book
Xenograft Data Catalog
The following section contains tumor growth data collected from xenograft studies that were conducted by Charles River Discovery
Services. This data represents various cell lines and histotypes implanted and grown in select Charles River immunodeficient animal
models and should be used as reference data only. Volume 1 contains xenograft data for the following models:
•
Athymic Nude Mouse
•
Fox Chase SCID® Mouse (C.B-17 SCID)
•
Charles River will continue to expand our data catalog in upcoming volumes to include additional data on immunodeficient animal models
produced by Charles River.
Nude Model
Athymic Nude Mouse
Strain Code: 490 (homozygous), 491 (heterozygous)*
Nomenclature: Crl:NU(NCr)-Foxn1nu
Origin: This immunodeficient nude mouse originated from NIH and was originally thought to be a BALB/c congenic. It was later
determined that it was not inbred and is, therefore, maintained as an outbred. This model is not associated with any stock or strain.
The homozygous animal lacks a thymus, is unable to produce T cells and is therefore immunodeficient. To Charles River from NCI in 2010.
*Heterozygous (haired) animals are not immunodeficient.
Severe Combined Immunodeficiency (SCID) Models
Fox Chase SCID® Mouse (C.B-17 SCID)
Strain Code: 236
Nomenclature: CB17/Icr-Prkdcscid/IcrIcoCrl
Origin: A congenic mouse that possesses a genetic autosomal recessive mutation (Prkdcscid) that was discovered in 1980 by Bosma in
C.B-17/Icr mice at Fox Chase Cancer Center. SCID mice show a severe combined immunodeficiency affecting both B and T lymphocytes.
They have normal natural killer (NK) cells, macrophages and granulocytes. To Charles River in 1991 from a Charles River France
foundation colony.
Strain Code: 250
Nomenclature: CB17.Cg-PrkdcscidLystbg-J/Crl
Origin: A congenic mouse that possesses both autosomal recessive mutations SCID (Prkdcscid) and beige (Lystbg-J). The SCID mutation
results in severe combined immunodeficiency affecting both the B and T lymphocytes. The beige mutation results in defective natural killer
(NK) cells. This mouse was developed by Croy et al. at the University of Guelph by an intercross of C.B-17 scid/scid to C57BL/6 bg/bg
mice. To Charles River in 1993.
Immunodeficient Models Xenograft Data Catalog - Volume 1 Research Models
2
Human Tumor Xenografts
Bladder
SW780 Individual Tumor Growth
Model: Fox Chase SCID® Mouse (C.B-17 SCID)
Tissue: Bladder - Cell Line: SW780 - Treatment: None
Brain
U251 Individual Tumor Growth
Model: Athymic Nude Mouse
Tissue: Brain - Cell Line: U251 - Treatment: None
Research Models
3
U-87 MG Individual Tumor Growth
Tissue: Brain - Cell Line: U-87 MG - Treatment: Vehicle
Technical Book
Breast
BT474 Individual Tumor Growth
Tissue: Breast - Cell Line: BT474 - Treatment: Vehicle
JIMT-1 Individual Tumor Growth
Tissue: Breast - Cell Line: JIMT-1 - Treatment: Vehicle
Immunodeficient Models Xenograft Data Catalog - Volume 1 HCC1954 Individual Donor Tumor Growth
Model: Fox Chase SCID® Beige Mouse (SCID Beige)
Tissue: Breast - Cell Line: HCC1954 - Treatment: Vehicle
MCF-7 Individual Tumor Growth
Tissue: Breast - Cell Line: MCF-7 - Treatment: None
Research Models
4
Breast
MDA-MB231 Individual Tumor Growth
Tissue: Breast - Cell Line: MDA-MB231 - Treatment: Vehicle
MX-1 Individual Tumor Growth
Tissue: Breast - Cell Line: MX-1 - Treatment: Vehicle (iv,qd x 1)
Colon
COLO205 Individual Tumor Growth
Tissue: Colon - Cell Line: COLO205 - Treatment: None
Research Models
5
HCT116 Individual Tumor Growth
Tissue: Colon - Cell Line: HCT116 - Treatment: Vehicle
Technical Book
Colon
HT29 Individual Tumor Growth
Tissue: Colon - Cell Line: HT29 - Treatment: Vehicle
SW620 Individual Tumor Growth
Tissue: Colon - Cell Line: SW620 - Treatment: Vehicle
Epithelial
A431 Individual Tumor Growth
Tissue: Epithelial - Cell Line: A431 - Treatment: Vehicle
6
Gastric
MKN-45 Individual Tumor Growth
Tissue: Gastric - Cell Line: MKN-45 - Treatment: Vehicle
NCI-N87 Individual Tumor Growth
Tissue: Gastric - Cell Line: NCI-N87 - Treatment: Vehicle
Head and Neck
SNU5 Individual Tumor Growth
Tissue: Gastric - Cell Line: SNU5 - Treatment: Vehicle
Research Models
7
FaDu Individual Tumor Growth
Tissue: Head and Neck - Cell Line: FaDu - Treatment: Vehicle
Technical Book
Leukemia
HL-60 Individual Tumor Growth
Tissue: Leukemia - Cell Line: HL-60 - Treatment: Vehicle
MOLM-13 Individual Tumor Growth
Tissue: Leukemia - Cell Line: MOLM-13 - Treatment: None
Immunodeficient Models Xenograft Data Catalog - Volume 1 K562 Individual Tumor Growth
Tissue: Leukemia - Cell Line: K562 - Treatment: Vehicle
MOLT-4 Individual Tumor Growth
Tissue: Leukemia - Cell Line: MOLT-4 - Treatment: None
Research Models
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Leukemia
MV4-11 Individual Tumor Growth
Tissue: Leukemia - Cell Line: MV4-11 - Treatment: Vehicle
SET2 Individual Tumor Growth
Tissue: Leukemia - Cell Line: SET2 - Treatment: Vehicle
Liver
SNU-398 Individual Tumor Growth
Tissue: Liver - Cell Line: SNU-398 - Treatment: None
Research Models
9
Hep3B Individual Tumor Growth
Tissue: Liver - Cell Line: Hep3B - Treatment: Vehicle
Technical Book
Tissue: Lung (NSC) - Cell Line: A427 - Treatment: None
H226 Individual Tumor Growth
Tissue: Lung (NSC) - Cell Line: H226 - Treatment: Vehicle
Immunodeficient Models Xenograft Data Catalog - Volume 1 A549 Individual Tumor Growth
Tissue: Lung (NSC) - Cell Line: A549 - Treatment: Vehicle 1
Tissue: Lung (NSC) - Cell Line: H292 - Treatment: None
Research Models
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Research Models
11
Technical Book
Immunodeficient Models Xenograft Data Catalog - Volume 1 H810 Individual Tumor Growth
Research Models
12
HCC-44 Individual Tumor Growth
Tissue: Lung (NSC) - Cell Line: HCC-44 - Treatment: None
NCI H2122 Individual Tumor Growth
Tissue: Lung (NSC) - Cell Line: NCI H2122 - Treatment: Vehicle
SKMES Individual Tumor Growth
Tissue: Lung (NSC) - Cell Line: SKMES - Treatment: Vehicle
Research Models
13
Technical Book
Lung (small cell)
Tissue: Lung (SC) - Cell Line: H69 - Treatment: Vehicle
Tissue: Lung (SC) - Cell Line: H211 - Treatment: Saline
Immunodeficient Models Xenograft Data Catalog - Volume 1 H82 Individual Tumor Growth
Tissue: Lung (SC) - Cell Line: H82 - Treatment: Saline
Tissue: Lung (SC) - Cell Line: H526 - Treatment: Vehicle
Research Models
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Lung (small cell)
SHP-77 Individual Tumor Growth
Tissue: Lung (SC) - Cell Line: SHP-77 - Treatment: Saline
Research Models
15
DMS-114 Individual Tumor Growth
Tissue: Lung (SC) - Cell Line: DMS-114 - Treatment: Saline
Technical Book
Lymphoma
Daudi Individual Tumor Growth
Tissue: Lymphoma - Cell Line: Daudi - Treatment: Vehicle
Granta-519 Individual Tumor Growth
Tissue: Lymphoma - Cell Line: Granta-519 - Treatment: Vehicle
Immunodeficient Models Xenograft Data Catalog - Volume 1 DOHH-2 Individual Tumor Growth
Tissue: Lymphoma - Cell Line: DOHH-2 - Treatment: Vehicle
Ramos Individual Tumor Growth
Tissue: Lymphoma - Cell Line: Ramos - Treatment: Vehicle
Research Models
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Lymphoma
RL Individual Tumor Growth
Tissue: Lymphoma - Cell Line: RL - Treatment: None
SU-DHL-4 Individual Tumor Growth
Tissue: Lymphoma - Cell Line: SU-DHL-4 - Treatment: Vehicle
SU-DHL-6 Individual Tumor Growth
Tissue: Lymphoma - Cell Line: SU-DHL-6 - Treatment: Vehicle
Research Models
17
Technical Book
Melanoma
Tissue: Melanoma - Cell Line: A375 - Treatment: Vehicle
Tissue: Melanoma - Cell Line: A2058 - Treatment: Vehicle
Mesothelioma
SKMEL-5 Individual Tumor Growth
Tissue: Melanoma - Cell Line: SKMEL-5 - Treatment: Vehicle
Immunodeficient Models Xenograft Data Catalog - Volume 1 MSTO-211H Individual Tumor Growth
Tissue: Mesothelioma - Cell Line: MSTO-211H - Treatment: Vehicle
Research Models
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Multiple Myeloma
Tissue: Multiple Myeloma - Cell Line: H929 - Treatment: PBS
OPM-2 Individual Tumor Growth
Tissue: Multiple Myeloma - Cell Line: OPM-2 - Treatment: None
RPMI-8226 Individual Tumor Growth
Tissue: Multiple Myeloma - Cell Line: RPMI-8226 - Treatment: None
Research Models
19
Technical Book
Ovarian
Tissue: Ovarian - Cell Line: A2780 - Treatment: Vehicle
OVCAR3 Individual Tumor Growth
Tissue: Ovarian - Cell Line: OVCAR3 - Treatment: Vehicle
Immunodeficient Models Xenograft Data Catalog - Volume 1 SKOV3 Individual Tumor Growth
Tissue: Ovarian - Cell Line: SKOV3 - Treatment: Vehicle
TOV-21G Individual Tumor Growth
Tissue: Ovarian - Cell Line: TOV-21G - Treatment: Vehicle
Research Models
20
Pancreas
Bx-PC3 Individual Tumor Growth
Tissue: Pancreas - Cell Line: Bx-PC3 - Treatment: Vehicle
MiaPaCa-2 Individual Tumor Growth
Tissue: Pancreas - Cell Line: MiaPaCa-2 - Treatment: Vehicle 1
Research Models
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Capan-1 Individual Tumor Growth
Tissue: Pancreas - Cell Line: Capan-1 - Treatment: Vehicle
PANC1 Individual Tumor Growth
Tissue: Pancreas - Cell Line: PANC1 - Treatment: Vehicle
Technical Book
Prostate
CWR22Rv1 Individual Tumor Growth
Tissue: Prostate - Cell Line: CWR22Rv1 - Treatment: Vehicle
PC3 Individual Tumor Growth
Tissue: Prostate - Cell Line: PC3 - Treatment: Vehicle
Renal
786-O Individual Tumor Growth
Tissue: Renal - Cell Line: 786-O - Treatment: Vehicle
22
Sarcoma
HT1080 Individual Tumor Growth
Tissue: Sarcoma - Cell Line: HT1080 - Treatment: Vehicle
SJSA-1 Individual Tumor Growth
Tissue: Sarcoma - Cell Line: SJSA-1 - Treatment: Vehicle
Thyroid
8505c Individual Tumor Growth
Tissue: Thyroid - Cell Line: 8505c - Treatment: Vehicle
Research Models
23
FTC-238 Individual Tumor Growth
Tissue: Thyroid - Cell Line: FTC-238 - Treatment: None
Technical Book
Uterine
MFE-280 Individual Tumor Growth
Tissue: Uterine - Cell Line: MFE-280 - Treatment: None
24
Cell Line Testing – 360 Diagnostics
Research biologics of rodent or human origin are often introduced into research animals as part of an investigative procedure. To help
maintain the validity of your in vivo research, it is important to perform precautionary screening on your research biologics to confirm that
they are free of infectious agents and that they are originating from the appropriate host species. Charles River provides TaqMan® PCR
testing on the OpenArray® platform to identify rodent and human infectious agents in any of your research biologics. TaqMan® technology
is ten to one hundred times more sensitive than traditional gel-based qualitative PCR, and the use of an internal probe provides
incomparable specificity. This technology allows samples to be analyzed without opening reaction tubes, which prevents the release of
potentially contaminating PCR products, a common downfall associated with gel-based PCR assays.
Rodent CLEAR Panels
PCR Panels to Screen Cell Lines and Research Biologics for Rodent Infectious Agents
Agent
Mouse
Essential
Rat
Essential
Mouse/Rat
Comprehensive
Murine norovirus (MNV)
•
•
Mouse parvoviruses* (MPV-1, MPV-2, MPV-3, MPV-4, MVM)
•
•
Mouse hepatitis virus (MHV)
•
•
Reovirus (Type 1 & 3) (REO)
•
•
•
Lymphocytic choriomeningitis virus (LCMV)
•
•
•
Lactate dehydrogenase-elevating virus (LDV)
•
•
•
Mouse rotavirus (MRV/EDIM)
•
•
Theiler’s murine encephalomyelitis virus (TMEV [GDVII])
•
Mousepox (Ectromelia) (ECTRO)
•
•
Hantavirus hantaan (HANT)
•
•
Hantavirus seoul (SEO)
Polyoma virus (POLY)
•
•
•
•
•
K virus (K)
Mouse adenovirus (MAV-1 & MAV-2)
•
•
•
•
Mouse cytomegalovirus (MCMV)
•
Mouse thymic virus (MTLV)
•
Pneumonia virus of mice (PVM)
•
Sendai (SEND)
•
•
•
Rat cytomegalovirus (RCMV)
•
•
Rat theilovirus (Theiler’s-like virus of rats [RTV])
•
•
Rat parvoviruses* (RPV, KRV, RMV, H-1)
•
•
Rat rotavirus (IDIR)
•
•
Rat coronavirus (RCV, SDAV)
•
•
Mycoplasma (genus) (including Acholeplasma laidlawii)
•
•
•
Mycoplasma pulmonis
•
•
•
Positive template control
•
•
•
Negative template control
•
•
•
Spike inhibition control
•
•
•
Nucleic acid recovery control (NARC)
•
•
•
*Strain determination assays are performed on all positive results.
Research Models
25
Technical Book
Human CLEAR Panels
PCR Panels to Screen Cell Lines and Research Biologics for Human Infectious Agents
Agent
Human
HEP/HIV
Polyomavirus (John Cunningham virus)
Human
Essential
Human
Comprehensive
•
•
Polyomavirus (BK virus)
•
•
Herpesvirus type 6
•
•
Herpesvirus type 7
•
•
Herpesvirus type 8
•
•
Parvovirus B19
•
•
Epstein-Barr virus
•
•
Hepatitis A virus
•
•
•
•
Hepatitis B virus
•
Hepatitis C virus
•
•
•
Papillomavirus type 16
•
•
Papillomavirus type 18
•
•
Human T-lymphotropic virus
•
•
Human cytomegalovirus
•
•
Human immunodeficiency virus type 1
•
•
•
Human immunodeficiency virus type 2
•
•
•
•
•
Adeno-associated virus
Human foamy virus
Mycoplasma (genus) (including Acholeplasma laidlawii)
•
•
•
•
•
Lymphocytic choriomeningitis virus (LCMV)
•
Hantavirus hantaan
•
Hantavirus seoul
•
Positive template control
•
•
•
Negative template control
•
•
•
Spike inhibition control
•
•
•
Nucleic acid recovery control (NARC)
•
•
•
Contamination CLEAR
The Cell Line Examination and Report (CLEAR) PCR Panel allows you to check the identity of your cell lines. It can detect inter-species
contamination of less than 0.5% using TaqMan® PCR assays. Cells that can be differentiated include those originating from mouse, rat,
Chinese hamster, Golden Syrian hamster, human and nonhuman primate (NHP) species.
26
Discovery Services
As your scientific partner and preclinical CRO provider, we bring innovative thinking, flexibility and efficiency to your program at
any point from your very first research question about a target to a drug candidates’ first trial in man.
Did you know that Charles River supported the development of more than half
of the 41 new molecular entities (NMEs) approved by the FDA in 2014?
Our unique combination of interdisciplinary, multi-disciplinary drug discovery expertise and unparalleled scope of capabilities in
targets, platforms and therapeutic areas allow us to deliver scientific depth and breadth with insight and data you can trust to
progress your drug discovery programs at any point along the way.
Collection of Oncology Research Experiments (CORE)
The CORE (Collection of Oncology Research Experiments) is an online library of peer-reviewed publications designed to help you find the
most appropriate research model for your oncology cell lines. Search through the publications by first selecting your tissue type.
Then, simply select the cell line you want to reference and the publications will be listed by animal model. The publications were collected
from various online resources and are non-curated. Visit www.criver.com/core for publications.
Supplemental Services
Charles River’s global infrastructure gives you access to unparalleled products and services in the field of oncology. Whether you are in
basic research and discovery or ready to move your therapeutic into the clinical development phase, we are strategically positioned to
help you accelerate your cancer research and anticancer drug development efforts.
Safety Assessment
• General toxicology studies
Clinical Support
• Clinical bioanalysis
• Infusion toxicology studies
• Clinical pharmacokinetics
• IND-enabling programs
• Clinical immunology and
biomarker services
• Pathology services
• Drug metabolism and
pharmacokinetics
Manufacturing Support
• GMP-compliant biologics
testing
• Endotoxin detection and
microbial identification
• Pathology for clinical trials
• Laboratory sciences
Research Models
27

Immunodeficient Model Xenograft Data

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