1980-1981 - askjpc.org

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Results
AFIP l,IednesdaySlide Ccrference No' I
17 Septerrber 1980
CaseI - 78310
1668870
grodng firn nasq' -present for qte 1"T,-3
fiIs a slor gr
sPec
nruLulv: -"'îglfiliilid;p";J-fffiG
;f*i"i_ri*"a q"g... Thenasslrasdeeplv
-ii
sternum ot a
the
adherenr ro rhe *a"iiiing
tr"" iuice firm and itrite cr the cut surface'
tir"u6..
canine'
fleoarrgiopericytcma, . skin,
-*irich are
capillraries
*"ll
of
9
ff't"
celis that are considered to be
drorls of plemrp[i[-"Iili9t*r.tr9n"a
-iti"-lumr
surrounded by
stain
rirst ue iiiieiS"ti.i"a fio neurofibrmas. Reticurr-m
;;i;fi;;.
the-Presence 9f fatgg nuders of reticular
of the trenargioperi"yi-"-ao*ti"tes
The tLmr
small capillaries'
arourd-the
layers
iib;;; ;.."rE;e'i"-"6"certric
inccryletely
is
often
a
result
-d.as
usrjally infiltraled-itr.-*a.rryiG-lis;G;
excision.
following
recur
to
Etrem
f6r
It is ttoi -ccm,ar
"rJis"a.
Attendees' Diagnosis & C@nts:
skin, spaniel mix, canine'
: Hffipericytma,
'vL
Contributor' s Diagrosis E C@enggi
'.."-v''-o the diagrpsis- of hemangiopericytoa.
*"itrld
Aii fii""a".r
Gmnts:
fiblcma/sarcona' ancl
negrofibrma/sarcoma'
included
diagnoses
Oiffeiential
*totr#'ilI-d;e;i;ia
melancma,
of 1t1e1!1:,:ugiit:9i::f, ::t'
ryPg. e ntmber.
6u ttmrs"ot
ffifiHa ffili1#LJacffi;;fi"s'5i'""""i* by"ut*Jus,pindlg
]btrlton (3). Discussiqr corcernirg
as Erissified
[Jilil"iliotiu"osercma',
f..tuies of this u.frcr and a ietent retroiPeltlyt :!9-l
the electrqr nicr6;6i;
at Cornefl University, reclassifiei 50%of the tumrs
6;Gp,rtti"h"di-;*ttu"rca
Uaseddt the preserne of
t*tgiopericytciis
diaspsed ne'rofibrosarconas
"t
meoranes,.iliciotubirles, md acEin-filanencs'
are Presencqo! desuosres'
";iro;fil;;;;-b;;*nt of-tt.
Characteristics
""*ofiUf*"s/sarcmas
*a -r" rbrgh endoplasmicreticul-m dd
incmrece bascnt rrF.rl.rares,
doryaraiivestudieshavestrorn
"o["L*tin trfrndopeii"yrcr"g,
observed
;ilffiffiiiiii-*,
U"a":fikle iesedlance to canine hemangiorhar hu6 tt rattgioplii;F-1..
jr-ncEicrs which are not present in
pericytmas and Etrat *robe of tLilns-t"u" tigttt
redrtel $at incqlete excision was a
nrlber of
the canine t'-rl"-i
cantainedno natural borders'
ifti-"i*ined
possibility in thab-ttre sectims"tt*a*r
contribuEor:
-iez4 and Veterinary servicel: Lgl^+lgtles county
cooparative ldical
EricksonAve.,-Domev,cA 90242'
o"p"##iffi"1il-il;;iii,References:
f f i . H . L . a n-i.--sr"u
d N i e l s e n , s . . wpracr.
. j ^ ^ c * i n599-5M,
" ' B T * e iL967.
opericytcmas,asurvey
'ffiil,
of z.iii"l
2.
t
Animal
8:
C. and Hunt, R..D.:
Jqtes, T.
n]'n],
n. , Jqtesr
Smith, n.
T.-c..
Veterinary Sathologl' 4rh
""d-Iglt,R'^P:'^^@
iltz,'ge zo1-19;-'o
Edirion,Lea& riu?ei"lTi!il9pt i7,
an* tissue'
ri
the skin and sofc
A: A. and Pu1ley, L. T. ' thors,of
il"'sili.,L;;
Californi
california
of
of
g' lbrrt*,
University
university
lb'tton'
e.
,rrffe of
nf Dcrrestis turintels. editi'd'i' d'i.
InTurPrs
@
,PP
78-0594
CaseII - -Avear
of
1667115
's wallaby (Walrabla
the ssecctd
(Wallgbig -rufogrisea),
rulol4rlsea)' Ene
o1d female BenneEt's wallaby
go dcnm" in a zoo exhibiC area, appeared Eo be in shocK. 5ne !{as
unresponsive Eo trea@nt witb antibiotics, ffuids, B vitmins and corticosteroidF
and wis euthanatized after 6 days. Ttre animal was excessively fat. lilr iryressive
gross.lesions were described, althouglr pale areas were noted in the psoas and
gluteal urscles.
Laboratory Results:
TfiC
RBC
Hb
Hct
seg
Bard
L)ryh
l,brp
Alkallne Phosphatase
tDrl
Glucose
Potassirm
E['N
soT
.SGPT
Greatinine
Bilirubin
Calcir-m
Phosphorus
Albtrnin
Total Protein
4,810,000.
1 5 . 5da
45.9 %
93. 7"
2 . ollo
4 . 7"
l . 7"
198. vlL
3 , 7 8 0 . UIL
2 7 . ry/dI
6 . 2 meq/L
6 9 . ry/dl
3ul. vlL
1 , 3 1 0 . IJIL
1 . 0 mg/dI
0 . 1 ry/dl
8 . 4 mg/dl
7 . 3 qs/dl
1 . 9 sldL
4 . 4 El dt
4,930,000.
1 6 . 0 97"
4 7 . L%
60.
2. %
38. %
0.
29p. ulL
5 , 0 0 0 . U/L
8 8 . qg/dr
4 . req/L
48. qs/d1
5 , 0 0 0 . vlL
2 , 6 L 0 . UIL
0 . 5 qg/dr
0 . 2 qs/dl
9 . 9qs/dl
4 . 0 ry/dl
2 . 4 sldL
5 . 3 sl dL
ol
lo
Cqrtributor's Diagnosis ! Cqqgnqqr Dtyopathy(nyodegeneratiqr), subacute,
t's wallaby, possibly of Vit. E-Se
dif
dependent type.
A third wallaby treaced
Tclgue, esophagus, and skeletal mlscle reere affectd.
for a tbree week period before necropsy had heavy collagerous tissue betweenutscle
fiber bundles and lighcer connective tissue surrotnding surviving fibers.
Ll4hocytes and plasna cells were present in mrscle fro this animal, ad both
Subsequently, 2
cardiac and skeletal nrscle fibers had undergore mineralizatiqr.
"joeys", one out of the pouch, the other still nursing, developed shggishness and
The wallabies
but respondeddrematically to E-Se injections.
locoDtor difficulty
gtazed on pasture and were fed rabbit and rodenc pellets, greens, md dry dog
food. Bennett's variety consr.trFdall the dog food. AIl chree that died were
overfat.
Myodegeneratiqrand recrosis, acute, diffuse,
Attendees' Diapgrosis& Crents:
llbby,-marsupial. Variou,s diagroses were
seve
given including degenerative myopathy, subacuEen;psitis, and rhabdoyolysis.
Etiologic Diagnosis: Nucritional myopathy,Vit. E-Se deficiency.
i
Differential diagnosis rnst often given was "capture myopathy".
- 2 -
AttendeescqurenEedqr the acute nature of fhe lesiqr and lack of an
in the clinical ctrernistrieswith elevated
nU"ot*fiii".
inflanmat*y r"rp*r"ld;iirdA fu, Scpt, Ltft, initial potassir-urand BuN. Clinical choistries
;;t*r
Attendees
total protein, pholFo.g": t$-9"lciun'
with lor values ;;;;t-g1*o-",
g-se
deficiency.
was_msrcqrsistent wlitr vir.
-.Ihe history
iii".a it a ih. history'usulty
mrscular
and/or
restraint
indlcates-recent periods of
if,-;".ptui" ryop"*,yn
oly
resPonsive
ltas
in tfrese wallabies
exertim. rt *." ,#tJ*t"i"ttr.-"a,aitian
(5).
by
others
to massivedosesof Vit. E ad not to Selenir.mas rePorted
Cdrtributor:
IlepartuenC of Veterinary
Sciences, Universicy
of Arizona,
TucsdnF857zl.
References:
A. c. and Kemedy, G. A.: Degenerative myoPathyin a giraffe.
ffi",
JAVMA163, 551, L973.
and I'ffiaggart, C.: Wtrite m.lscle disease in the lbLntain
i. gerberi,b.-ll.
Goar. J. i,ndlife. I'!mt. 35: 752, L97L:
ard Arthur, D.: Dystrophic
3. Oren, R. R.,-I,bore, J. N: , Hopkins, Jr.B.
y3,
L977
l7t:
JAVf"lA
horses.
adult
in
myodegeneration
'
Veterinary Pathology,
R.
D.:
Hunt,
and
snith,
C.
T.
Jones,
H. A. ,
4:
p.
f049..
L972,
--- & Febiger, Philadelphia,
Lea
A-nyopathy in Goodfellow-Treekangaroos
5.- MaEKenzie,W. F. et-al.:
(Ilendrolaeu" eoodi"lfcmi) associated irittr cbnfinerent ard respcrsive to massive
ffi.ffifeeiaars
- syrposiumqr ccrpararivePathologyof ZooAnimals,
Natiqral TnLogical Park, 1978.
(ArrPur49r0).
casur - 27535
ocker spaniel
FUps, four died acutely
ages or 4 ano > weeks. No clinical sig;s of iiliress hrete noted.
hEarrt and liver of these anirnels were ieported to be enlarged.
+ a -
Contributor's Diapposis & Crenr|lE ;
betteen the
At necropsy EIF
I'$ocarditis, dissminated, subacute,ntianuclear inclusiqs consisEent with
,"u"
oarvovirus infection.
Crents will be forwarded pending EI'{results '
I'tyocarditis, acute, diffuse' severe' heart,
Attendees' Diagnosis & Crents:
cock
Etioiogic Diagnosis: Viral myocarditis.
EtiologY: Parvovirus.
felt the changes in the tissue sectiqr nere of a
Ccunents: S*-"ii""aees
peracute narure wtrile others believed the c6anges were mre of an acute to subacute
naEure, as severat aescribed inflrnrratory infiftrates and reactive sarcolemal
cells in che tissue sections. Discussioir of age-related lesions "i+.Pafvovirysln
signs and forms were held. The nryocardial form is usually totlr
una
"fi"ical
of rg" nrhereasthe intestinal forn is found in older dqls.
b';;d
;["-1.";-it."
- 3 -
Discussion of available
wiEn canine
diagnostic Cests included HA of swine RBC's
caution
e1y"yi:?1^rytticles'
as canine minure virus is
n,iucut
in-r.ii;;iliai'oi
!{as expressed
"L"*inaEion fo1 doe1, One attenCee
similar in rnrpholog)., and.is "oi'tii"i.J
".1e"ttttg"ti"
or canine farvovirus infection
cws=,,ted that Ehere have u"en z-io-"o"rito=a'""""3
in Ehe State of l'tarYland'
MD 2L7OL'
Pathology Division, usAI'tRIID,ForE Detrick'
contributor:
ot-i"Ili';.6i;;-it1
serunandelecrronmicroscopy
References:
dogs
death in youn:rg
A., Russell, R. G., Babuik, !: A.: Sudden L979'
ffiay(es;I,l.
JAVNA174: 197-1203,
by.paivwi;;.wirh uryocardiEis
ana;on9"'
C' L' ' Myocar ditis of pr oba bl y
F ., H a ski n s,l ''1 :;:;
2 . J e z y k, P . "uurJ
L979.
u!: L204-L207,
virat origin in pup.'oT-r"*rire-gE;, rell$
p.t
infection in
Parvovirus
t"Jones,
3. Eugster,-A: K., Beqde]g,nT n. and
dogs. lerfre rzr: I340-l34r,1978'
associated wich
4. Thqsqr, G. l,[. and Gagno",e. N.:. lanile^gasEroenEeritis
c"n. Vet' 't' L9z %6' 1978'
p"ruouir*:ilk;'"gen|.
case IV - M9-114 (AFIP Yl83l4)'
6qui1e' Fo,Ica^Tutt'ro weeks prio; ic Ene
pelvic limb-paresis' This was
adnriFiolt-Jnere had been a progressivg, elotgwrd
n o t i c e a b l y b r o r s e i n t h e - r l F . h l . r e a r w i C h ahad
- t e nbeeir
d e nobserved.
c y ' t o k n u c kcucaneous
l e ' - T h e r emuscle
wasalso
urine retention but no dribbring-or""ii."
che animal was first admiuted' it
responsewas reduced posterio, io-iJ:--Wh;
to rhe clinical signs in
.In "baifiott
couid stand, hovrever,novtiC was-paraplegic.
iuFc-tla"E peripheral spastic paralvsis'
rhe posrerior riibJl'rii"t"-"""
Laboratory Results:
*rrt
Non-contributory'
' chronr-c'
Meningoencephalon'ryelicis
-p*to'o6'
Contributor's Diagnosis & Ccrnrencs:
proLably sarcosDoridia'
CcutrEl;Its:
None.
sublgute' mulEifocal to
Attendees' Diagnosis-:! rlouupnts-r l) Meningcuryelitis'
d i f , i > - r y : i i t i i , n o n s u P p u nonsupput"tive''nultiiocal
r a t i V e , r n t r 1 t i f too diffuse'
c a I t o
diffuse, severe, equine. 3) Enc=epttrfitis,
severe, medulla, equine.
Protozoal'
3).pi+l?iT"sondii'
3:i:i3E;:'i;T3ili"#:i;kll''zr
t?r"ffr?!l?,:p.
s presenE
yga5:" i'ia
ffiH?l' vli illl"5ffi;'; ;-i" r i iv"ffip."ar"f,ury1
Etiologic
Diagnosis:
-:..^-
3
i,.^^nânc
"v"ri3";-*diFi;r,
"-
rFrln
r.rhnf
rissug
iri3s
DI€:
ccne
descrioin-o.
in descrioin-o'
used in
wereused
ils6 were
. VariSus modiiiers Srro
ho rlicrrihrrfed
s
case slideS.
disrributed case
in Ehe
oi:::tf L:[ E:ril
;#=""'o'i"a-;;:!lF?,
lltih:l:Ii' :::iliHT'"
-,:::
::::*::'
'il";eif;::;-i:*::?:^::,':?*.'lliil";-
;i*#::.Xi"ffi?!'ffi
::5';iForEec
idl';i:
3i!l?n.i-;;"
B:::::::
:!
:ii:;::l*:i:-i:::'.li]li:
?:#:li:Tli
;i
::il:i::?i):"lTJ";#ffi
u s i im
I; f :'i"
+p:i:;1-i5l^F;li:::"::;:*:
::#:
aLLstrus=.' LslryL"*""t"#'--il;";
Alr #.#J;';;;;;J-fi;;-ou'"ii'"a,
neurons'
;:f*::'iii
!:liy-:Yf1,"-::il::.1'
w€fe a:'t)ilal
repcited
chang'es
rhe affecced '
in size
throughouc
- 4 -
degeneratian, deuryelinatiql
necrosis in.the gray matteE. The mrptrclogy.and
weiep:ovided
sF;i"l ;i"ios. thau
sreinins Eeacri;'s="i"ililiiaii-#;-;;;ie,red.
ov;{ the exact etiology of
table cqrttou""y"&i;;;
were non-"*.rroiiioly.-c-r"
. ry- ;;ktt? pttf."t a Toxoplaslike
Eouine prorozoal ltyeioerrephafitit-imtl
T'-seJii L ** definitive agent'andA
or'sdliso wtrite a fbr others t"u.-ir,ili*li*ced
aisgage in rtre equ-ine
nufoer of reporrs have ueeg generil;;;ff;iit*?i6i"
irpculatiqr studieg'
resurts-wi*,-"n"9i"i
negarive
L #*--d;ir"r e-91f3i*tidae'
but the
"t"iil-r"i
lbsr aurhors agtee that- tle -ag,€niUefcrgsTo-ffie-Iaftsarcocvstis
a
sgqges-of
ai{prte. TtrEin@rrediaqE
senusris still .;;;;;
a ti[etv probability'
"f
dr.-i6;i-i.t*i"i"-"*,"ia"red
Ipecies witkr *re G;
of Florida' Box J-145' JIIIIG'
contributor: Deparrent of Pathologl, university
326L0.
cairffiille-F
Referernes:
fre61-.J.
andllcdd' D. C. : Toxoplasma-Iike errcephaloyelitis
Path. 11: 87-96, L974.
v"t'z:aT-:;:;,oi:'3:
l!'li.
in the horse.
in spinal
q:l:"*ephalitis
:.
Fy'":^p.?lgTll
coinell vrit. 68i U!7-,,)8,1978.
cord
toa
il" iltff 3?'il;#"ii:.''i.'"!.ir:,^n:h:.S#lftil+;isdue
aiselse'i?ffi'nL"".
-
,a
r-..l-a
ma
J. LruDey,\'' r.' vsYê' v' "'
-reuig L65..249-?55,,L?7!l:
:ozoanpaiasite resedling T' gF Jii.
Protozoan
LetEer to the Editor'
4. D"b.y, J. p.: Toxopl""rcrirf.iltoil"".
JA\',lA 165:
668, L5 Oct L974.
TI}ONff P. O.NEI,LL
Capiain, USAF',356(VC)
nelisrY of VeterinaEY PathorogY
Oertrmint of Veterinary PaEhorog)l
- 5 -
Histories
AFIP WednesdaYGonfererce - No. I
t7 Septe$er 1980
otv).
P 1668870
CageI-78310slow grovringfirn mass' 'i1re
Present for-l,MT-: dr tbe
ca
masswas.deeply
ri* dog..
sterrum of a iil;";5:;td
ilt;Jf.n"ie-ap-iEr quite
'J"rfvins'tissrEe.
ar the cut section.
nhite on
tirt
fir;4tda,A t*rite
]-it-fi"-_--quit"
It was
l,remderlying t
adherent to the
1667lU
CaeeII - 78-05% (WaUnblq
rufoFrlsea), -ttte scand of tq=
'
6 vscrl "" ';-o;
was t'respctsive
;i;fii;
to
ar98r -appearerl P_extrtbit 8I98r.apryareo
to "gddctn"
"go- dorn" in a zoo extribit
--^-r--r corticosteroids
am..iaa
and
and
vrtains
fluid";'B-cqUx
-d;i'ar
r{ittr antiblotlcs,
Eo treaffiir
imressive
EBP++5#4'#"#
fat. lb ----r -t..r,
nLserceseivelv
in psoasind gluteal
rpt"a
p"i" ."o"-',ni
;;":ftffi;iilii'"ii;;-6-i;i;:
F#"i:?if,;"#t!'i!""Iitil'ariiorytr
mrscles.
LaboraEory Results:
l.lrc
4,810,000.
15.5
45.9
93. T
2. 7
4. x
1. z
198. ulL
3 , 7 8 0 . vlL
2 7 . q/ql
6 . 2 req/L
6 9 . q/dI
30/+. ulL
1,310. tilL
1 . 0 q/dr
0 . 1 EE/dT
8 . 4 ry/dl
7 . 3 q/dI
1 . 9 sl dL
4 . 4 s/dr
RBC
Hb
Hct
seg
Bard
Lvryh
lbno
Alkalire PbosPhatase
UH
Glucoee
PotassitD
Bt'N
sor
SPT
Creatlnine
Bllirubin
Calcitm
Phoephorus
Albuin
Total Protein
CaseIII - 27535-
1714910
*"r1."î;':d;i!i:G
;[*iiiffi!""!-1o.J.-
o
it"r"
*ti*lg
4,930,000.
16.0 97"
4 7 . L7"
@ . 7"
2. T
38. T
0.
299. vlL
5,000. ulL
88. ry/dr
4 . req/L
48. q/dl
5,000. ulL
2 , 6 1 0 . UIL
0 . 5 ry/dl
0 . 2 ddr
9 . 9 qs/dl
4 . 0 q/dl
2 . 4 EI dL
5 . 3 sl dL
5^
beq3 :t_:s-,"f.1.j
F.rpr,four died ?gutely
or
liver
at r-ciopuyttretreart-and
were reSrted to be enlarged'
CaseIV - N79-U4
1718314
Ltrsts rrq
there.had
aqtruuur(rl
EIfe admissial
EO the
to
Dtlof
Wegfg prior
Z neeks
abOUt 2
about
in tte
rprse
noEiceably
Has
pr"""i".' This
beena prog,ressiil,'p.IiJffi'p"i;i.-1is
th ;-i*Ieilt t":k',rili - ^*:: 11"-".11i::*::T :T^H;"
i ieiit1E""";i
gy!ffi,
iiftiiri"i'oiîI ii"ffi&
uLllJ|,lrl{5
v!
eL.
- ii""t afoitted,ît
Hr"- :Tm".n'g1
could stard, trcnrever,ncni'
"b+;iJ:
there
there
lids,
posterlor lids,
i.- rlya alinical
elnnrg in
in ttre
ttre Dosterlor
I,itt*t-ih;;i*i
to
L\r' t-+..|. -IIJL
Dosrerior
Lf,rl'Le|.
i-,- ----1--rr- ^ll{r{-
lG
to ";e
rn additt*
ir was oarmlesic.
"li"icar
paralysis'
spatial
wag
ias lefl-siied"peripheral
eigne-
T[},otr$ P. O'I\EILL
Captaln, IJSAF',BS0rc)
iei.tstri of Veterinary Pathology
Oertruint of Veterinary Pathrlogr
Histories
AFIP iHnesday Slide Ccrfererne - No. 2
24 Septeurber 1980
CaseI-78-1850-
1667020
-oi-Etr" l:M.
.a slaught
_ftre;f hd
ircen taken frcm
red
shoned red
pigs -srarghtered,
slarghtered, 5 stroned
18 pigq
7-rcnt1r.ord boare frcm rlre r* rc"al
a
with
red
irere
in dircter,
;p"riA ti&reys. The lesions reasured 2 rs 5 m in-dircter,
parerrchyna.
the
frcm
slightly
protruded
and
t4ttitlsh center
case II - 7912080-1- (4EIq-UE948).i"i.r*
umiiirg *a
"6u"t"
euthanasia.
requested
s pre-sentedwittr a history- of frequeit qrner
for trp'days.' vaccinatiqr higtory mrna{n- ltte
Iaboraory Results: Wrc r,000/ul,, wI 24Z,II-b-8.249lt
RBC2.4 X 106/U!,
n riffitJGr_56,oob,_3.
lqrgg{ceus, Fikilocyrosis. urinalvsis:
ry/dl'
lF-i:Or3;'3+sili"ubin, t0-U nrc/pr. ntN-1'98
CaseIII - 79-3U1 -
1713%9
y denived ad naintalned licter was infected
ui3-i"it1ryIigTLi:Y:2;^ ^t' 22
of age
at 7 daye
-q262-Jiiain>
"l*' "rilii"li-qtt
doreropeiwithout liparent-nanrologic
davsprD. acurer""pi"iioii-aifiicuity
ll
rrss6re
vv6'
wittl
per
cardiac perfirsion
;:'-;.:;i;;;
;;';;-;l
bv cardiac
extieolg by
tn extis'rfs
euthsrized in
vni i,'tttr"i"i
et;i"=ti*-qta-dre'dog
fixatlve at 24 daYe PID.
CaseIV - A-19735- (AF'IPylffi7Ln>.-
wlth diarrhea of np mrths
$ n_reggnred
duratiqr. eec@ seveie aurfng the lait five dayg plior to Presentatiqt'
Truicaied nid-abdcnrnir-masgaird gastrointestinal
n;;i;;i'"r,a-"riliAi-*ti:,rp
g*ploi"t*y rril rEcropsy reveaLedintrahminal-pedtnrulated IIErssat
obstrrrtlqr.
ic tlah nodes, ceco-colic intr.ssuscepticr and
it"*"*i-j,-.iifi;-;1;ig;-*".otE
pintorre.
TTT.OIIffP. O,NEILL
Captain, USAF',BSC(VC)
neiistry of Veterirrary Pathologr
Defaruidt of Veterinary Patholqgl
Results
AFIP lJednesdaySlide Conference - No. 2
24 Septeder 1980
CaseI - 78-1850_:_(4FI! L667020).
-a slaugfrter-house. Theyhad been taken fro
7-mrth-old boars fro tlre s''E herd.
Of Ehe l8 pigs slaugLtered,
-*"
f shotrediJ
sPgtg+ kidrnys. ltre legiqre reasured 2 to 5 - in?irci&-, --' - red wirh a
r{tritish center and protruded stiglrtly fr@ the parernhyua.
laborator
lesiorts:
ccttributorrs
rctiffiEdlltriilEpp.
Actfrobacillug-like
Dlagnosis & c@rts:
infectian
@
occas@swine.
Attendeegl
organlsrm nere isolated frm ttre
septic edolic
nephritis associated with
ls not uncqmrxr in piglets and has been reported
ig & Conrents:
en6olie, suppu'rative, u.rltifocal, mderate,
kidney, pig.
Etiologic Diagnoeis: Grm negative bacteria.
Etiology:
Salme1La spp. wai dre favored etiologic agent mrtiqred, wtrile
w!r'--r
several others ?Afffifali!
preferred such o"g*rirn[-;"E*oehik
;,td'
-Pasteurella.
Connpnts: The particiPants discussed the distributicn of ttre lesicts and felt
the rultifocal nature and ctoee aesociatiqr of blood vesgels was cquistent wittr a
sepEiceoic-cqrditian. lGntion waemadeof glrerular irvofvfit
and s@-f;it'
glcerulitlq,
also '=Jre appropriate diftses.
-and glrerulorcphritis
Itre Grm
sEail Ewid{
ctre cqrtributor dmetrrated cih iregarive uiciffi, wtrictr-mst
9y
participants. felt,we1e coqatible with Salmrellq spp.1s this is thi 69et conrrnly
isolated pachogerntc.Gsanigative-o:gariiffFoffiin'ltrir-rSi"r.
grher bacterial
€entg associatedwith septic-conditiqrs in swine sLtchas:'E.-=.-*'r-&-';'-coli, Erysi;folfirix
spp. and streptococcusspi. also rrere briefly discussed.Cqrtributor:
St. @c,
Anlrnel fathologl_Laboratory,
- - - - l ' \ Qr-ebecDept. of Agriculture,
C a n a d al Z Y r c a . -
Referernes:
fffirffii
R. E. eE a1.: Porcine abortion caused by
L equuli. JA\,!A 169:
7M, L976.
. -2- Bouley, G.: Ettde d'une--sounched'Actiorpbaclllus suis (Van Jartheld) .
isolee en Norndie.
Rec.r&d. vet. t+zt z]ffi:
3: Edwards,^l: R-.^ggdTaylor, R. L.:
Cornell Vet. 3z 393, L94L.
Shie6fG equirulis infecricr in a sow.
ur3948).
Case II - 79N2080:L-:
of frequent
, was presenued
'-v;;;i;ticn wiEh a history.
The offirer
urnknom'
history
icterus for cwodays'
uo,ili"frna
"6u.te
requested euthanasia.
aner,
I-aboratory Results:
Reri@9let-
S f.Off,-3+ Bilirubin,
2'4 x--106/UL'
!'lBC 23,000/uL,PcIl 247",Hb 8.2 gldL, RBc
3+l"ie.t
5o,o0o,
""lls,
mg/dr.
t0-f5 RBC/HPT. BUN-198
poTkilocytosis'
Urinalysis:
Cqrcributor's Diagrpgis & Go@ngsi .Lepgospitg-:i:'--,1:r$|]!g.,*erimal
disiefuinated
was
ffi..a1-'fr;;-;;"
or
* it. "..*.i surraces
ffid"--a "ia"rY
ffiTffiiloffi:'fi"tt;"
Hffiffit::""g9
thora"i"
the
ItiT::::;
"na-"ila?i;"1;ilfri-;;#;
ffi iilH'-,il;'il;;;-#E;a
-il9i:.*3
?i3
#'y
slisbrrvDre
beingfor ff1P:ffi:
excepr
;,*i
iii"ur"l--n,ttttt rFph"nodes !{ere dark red'
--1 r..L..l^necrosls'
degeneratiqr' necros'
tubular raoanorqri.n-proxinal
Microscopic ieiraf lesiqts
by
chaiacterized
t*pttitis,
"*ti"t"a-of
mrlrifocal mineralizarion, diff;;-i;t"ttiiti.f
edeoa an
nacrophaees;
and
of t)ryhocil";;i;ryt,9"1tt'
upderaEe infiltrariqr
Ttre
of t;oatocwes'
severe individGrizariqr
Li;;;;h;a'aiirG"
hmrrhages.
llicroscopic
wirh sever;;;td;ophagcyti"i".r.ruEh nodes were markedry,horrhagic
or canine
r*.r"H"-"o*iaered charlcreristic
i#hi";.ii'i[!-ria""i
-+
at the tire of necroPsy
leprospirosis. p.tii-ii"id e:rminaiion of urine collected
reieal6d nutErous sPirochetes'
t'"'
*d"H;il"i;fi:y;^:fiiil:'
interstitital'
subacrrte' diffuse'
zi'n'"tioJir;G;-eissociatiqr, diffr.rse,severe,1iver.
EEiologic Diagnosis: . l,ePtosPirosls'
sPP.
ar
t
_ _^^r _-rrl^ rLa
ânrril'rrnrrq
diacnbsis.
diagrosis, and
with the contributor's
agreed
ururninpusly
[ttendees
cmrts:
interstitir'm
infiltratiqr of ttre
hisrorogic findii!,sl--i; "aaitrd-;6 ;f,;-difi,,se
atqg"tration' sre aEtendees
tubui"i
pi"*imal
wirh l14hopr"*irEiii"^""il"-*a--ct.
The changesin
inEfma.tiqr'
felt rtrere was a tild early tiU.o"il ilG;ytlE-.tltb ba h"ighry ?uggesliy-eof leptospirosis'
the tiver cissue ;;;; di;;it""a-*a-feit
i" triet-fudy t@rature Exrvrnimic this
whit-*r"i.
archouglrorler
degeireraticr alang
;;#t""JirLr
;-;-p";"tbi;
airendees
charge. A rew "riaiii*"-in
chrqric
caline
"*riJI
role-.t -i9p959piral orginisns inwith the dissoclation. ttre
ad against
for
borh
*; aiscussed-uiiefiy and vrarpoints
inrersririar nefrii;i;
Etiology:
l€pto?piria
were e:(pressed.
contributor:
sardEus@-
Llniversity'
Departrent of veterinary Pathology, Louisiana State
70803.
References:
leptosoirosis'
n. and Cousineau, G. : . The patkrogenesisof
ffi,
cat.'J' c@P' lbd' 4r:
r. nmrrhages in experimnar ilircrpirori"'ir';iil;-pigs.
L976.
174-181,
of leptosoirosis.
2. Higgirrs, R. and Cor.rsineau,G.: lhe.pathogerresis
cair' l' cq' l€d' 41:
pigs'
rr. Jat6dicein experinentarreill"pitoti"-$ dfi;;
182-187, 1976.
leptospirosis: An imtrp3. lbrrison, I^l. I. & rrhight, N' G': -Cgnile
au"-i; 5 canicora. J- Pathol' u0:
pachological sEudyof inEers*i.iLi'nlprt.ilG
8l-aq, L976.
- 2 -
- 79-315L'
P L7L3949
derived and maintained litcer was infected
@ly
aE 7 days-of age with viiulent CDV(P252 scrain) via intraperitoneal rouEe. At 22
developed without apparent _neurologig
days PID, acute respiratory difficulty
dysfunction and the dog was euthanized in extremis by cardiac perfusion with
fixaEive at 24 days PID.
CaseIII
Cqrtributor rs
is & Cments:
l"tyocardial necrosis, urltifocal,
lfultiple small r{hitish linear streaks were resEricted to the ventricular
uryocardir-mand lungs appeared ed.'nntous. Sinilar gross and nicrosco_pic fgsicts
were noted in dogs-frm 4 similarly treated litters with 1-4 dogs affected in each
litter.
No lesicrs
were noted in age matched uninfected
parallel
cqrtrols
or in
dogs receiving non-neurovirulent tissue culture adopted CDV(R25?) via the sc
route at the s-rp age. In addition this dog trad a viral enceptralopathy with_ _
inflsrrqatory cell
widespread glial anil neuronal infection without dmrstrable
pner-mtia.
tissue depletim and mild interstitial
response, generalized lphoid
Ultiastructr.rral exaination of cardiac lesiqrs desrcnstrated intracytoplasmic
aggregatee, rcrphologcally ccEpatible with GDVparayxovirus,
tubular filrentous
macrophagesand intact
restricted to degenerating uyocytes and interstitial
Previous in vitro studies have shcnnrthat
tryocytes at the periphery of lesiqrs.
a depression of both cell
neonital dogs inlectirC with virulent R252-CWexprTffi
mediated and hr-mral inrqrrologic responses along- with a cell and plasna associated
sryles fror invr:lved litters loere tested weekly for Ca,
virenia.
Serr-nry'plasma
POaand I'[ Ievels and terminally for selenirm, glutathiqre peroxidase and vitoin
E levels.- A11 levels were within normal limits copared to conventimally raised
dq3s. Sliifrt elevations of SGOIbut not CPKwere observed but did not correlate
the possible contributory role of CllS involvwrt,
with occurience of lesiqrs.
sliglt anenia and depressed fummologic respansiverrss in the developurentof these
fro
lesTons is rpt kncrn. The lesiqls piesentei here must be differentiited
canine parvovirus ulyocarditis.
Attendees' Di
is&
Etion and necrosis with mineralizaticn,
acute,
unrlcifocal, rcderate, teart, canine.
Etiologic Diagnosis: Viral myocarditis.
Etiology: Canine Disteuper Virus.
Ccments: All attendees agreed with the contrioutor's diagnosis and etiologic
agent. Discussion of viruses that cause nyocarditis in othe-r species irclulded Fot
and t'louttr Disease in cattle (picornavirus), Encepbalcmyocarditis in swine
(picornavirus), Coxsackie - B virus in neonatal mice and coconavirus in mice and
rabbits. Also discussed were Ehe pathogenesis of calcification and the early
anoxic changeswhidr may be confused with or secandary to viral myocarditis.
Contributor:
I'GdicEiF-T€-fio
Deartnent of Veterinary Pathobiology, College of Veterinary
State University, 1925 Coffey Road, Coh-dus, CH 432L0.
References:
S. and Wallace, A. L.: Lyrphocyte associated fumuneresponses
T-ffiffika,
and reasles virus in disueuper-infected gnotobiotic dogs. An. J. Vet. Res. 40:
669-672,L979.
2. Higgins, R. J. et al.: Canine disteryter virus-associated cardimyoPathy
in the dog. (In Press).
- J -
@
Ehat becre
shepherd was presented wich diarrirea of Ewo
severe during the last five days prior to presenEation.
Laboratory Results: Physical and clinical rrcrk-up indicated a nid-aMminal
- - obstruction.
Exploratory surgery and necropsy revealed
mass@l
an intrah-uenal pedunculated mass at the ileocecal jr-rrcticr, enlarged resenteric
flqh nodes, ceco-colic intussuseptim and wtripornrs,, (previous history given
No cutaneous neoplasmsldere seen.
indi.catirg pinrooros was in error).
Histologically neoplastic cells also were seen in the lyqh rndes, bcte urrow,
spleen, liver and lungs. Ttrese cells were confirred via electron microscoPy :rs
well.
is & Cqrents:
ileocecal valve.
nast ce
, intestire,
The nedplasm is considered nalignant because of the presence of
Crent:
neoplastic cells in ottrer organs. Prinary mast cell tr.mrs in the intestire have
been described in cats, but no references liere found for the dog. Ttre nast cells
seen in this dog are similar to cutaneous neoplaernc, in ccrtrast to the cells in
the intestine of cats.
Cqrtributor's
Attendees' Diagrrosis & Cdrents: I'tast cell tr.mr, malignant, large intestine,
is neoplasm included malignant mastocytoa and
cani
mastocytosis.
There was total agreesent with the cantributor's diagnosis. Gisa
Crent:
stained sections of the tissue revealed nurErous cells containing various nuders
of Giensa positive intracytoplasmic granules, consistent with mast cells. Ttre
associatiqr of eosinophils and rnest cell tr&rs was discussed ctd is cmsidered to
be dr.rcto chemtactic- factors for eosinophils elaborated by nast cells as well ag
other eoeinophil atrracting factorg such as C56, C36, Iyuphokines ard histam,ine
(neakly). Vaso-active anine release with resultant edeurausually associated with
mast cell trrDrs also was discussed.
ConEributor:
Ttre Animal lGdical Center, 520 E. 62 St., NewYork, I{Y 1002f.
Refetence:
Distinctive
Ffrot;-T. et al.:
Lab. Invest. 332 L59-L67, L975.
intestinal
mast cell neoplasre of destic
TI}'ITHY P. O,NEIII
Captain, USAF,BSC(W)
Registry of Veterinary Pachologl
Deparmnt of Veterinary Pathology
- 4 -
cats.
Histories
AFIP llednesday Slide Conference - No. 3
I October 1980
Case I - U1696- (AFIP L755478).
rhesus urkey born and reared in a p-rr11
anorectlc,,
becm
sr.rddenly
ttrat
colcry
!e*, hypochernic srd detrydrated- Ileqeite
d was
6 days after the anset of syryto
aninat
died
tte
treatrnent,
s)lrytlmatic
necro,peted.
tibo.atory Resultsl the bematological values were witttin nornal linits stcePt
(r5%). s1t40-wasisolared frcm the spleei posmrtem.
for @
Case II - cA5-197 - (AflP L755476).
died ac992 days of age. A yellmish
ffiffirer
at its gleateet dirIErlsia|s
drite nodular nass rrcasurlng about I cm direter
surrounded the uEerus ard colqt.
CaseIII - 71537- (AnP U12658).
polttacirn birds in a pet shop *rich died
follorirg a brief illness narked by-pqofgseglgen watell.diarrhea. Thesebirds
of dmstically-raised
becare sick me weekafter the arrivil of a shiprt
parakeets.
and
cockatiels
Case IV - 27469- (aFIP Uf4918).
canine was presented qr 25 Jn 79 because of
On
vmirirg, over the previore 3 days. It respondecit9 spPtgPatic trea@ntIt was
previous
week.
the
drrring
voiting
cqlstant
after
30 Apr 7g it was riturned
levels
nitrogen
urea
Bl@a
detryiirated.was
narkedly
fid
dipressed,
.r"di"c.a,-u.iy
at
and
atteryts
a&inistratiqt
fluid
intraverpus
iesplte
remained 6tevaled
'!be
arner reqLEstd euthanasia. l{ecropsy dme by the
oericoneal dialysis.
Llintctsr reveaied pale firn ki&reye ard rp other abnormalities.
Laboratorv Results:
Dareffi
27 Jn 79
30 Apr 79
I laay 79
45
48
7
9
l,|rc
BrN
Na+
K+
15,000 (84%Seg)
5,700 (83%See)
19
126
110
r43
L43
3.5
3.7
G[,U
35
20
93
75
TII'OIIIY P. O,NEILL
Captain, USAF',B$(Vl)
of VetetinatY PathologY
tegistry
Departmnt of Veterinary Parhologl
Results
AFIP ttednesday Slide Conference - No. 3
I 0ctober 1980
Case I - 13696 - (AEIP L755478).
old male rhesus mrkey born and reared in a
Historv: Ti
pridffildry
that suddenly becre anorectic, weak, hlryothernic and dehydrated.
the animal died 5 days after cLteonset of syrytms
Despite s)rrytcmatic trea@nt,
ms
necropsied.
and
Laboratory Results:
#
for @
Ttre hemaEological values were within norual linits excePE
(U%). SV40-wasisolated from che spleen pos@rtem.
Contributor Diarnosis & C@tts:
, mrltifocal, maked.
2) Kidney, renal tubular cytregaly, with inclusions, mrltifocal.
3) Ki&ey, chrcric pyelitis, mild.
Besidee the renal tubtrlar necrosis, the aninrel aleo had a severe
Cmnts:
preuwria with similar associated basopttilic intranrlear
patchy interstitial
inclusiqs.
these inclusims !{ere positive for SV40antigen by immoperioxidase
staining of paraffln sectlone of affected organs. Electrqt microscopy revealed
characteristic papwavirus particles in these tissr.ps. This is the first reporc
indicating that'S'imian Virui 40 nay prodtre pulrcnary and renal disease in its
nacural hoet.
A t t e n d e e s 'D i
is and
diffuse, sevete with intranrrlear
6Sfltubular,
inclusi6n boiltes anil mrltlfocal tubular regeneration, kidney, rhesus mrkey.
Etiolosic Diagrrosis:
Diaenosis: Viral renal tubular necrosis.
Etiologic
virus.
EtiologSl: Si\140
Ccnnenib: The abore mrphologic diagnosis was the consensus follorirg
considerable discussion. Attendeeg' diagnoses turrrcd in before the csrfef,ence are
subacute, difftse,
listed in order of frequency: l) NephriEis, interstitial,
rnphritis, subacute, diffuee, mrked, kidney.
severe, kidney. 2) Tubulo-interstitial
sr.lbacute,diffuse, upderate to severe, kidney.
3) GlmrulonephriEis,
Attendees ilso listed the chronic pyelitis as either a separate mrphologic
diagnosis or included it in their primary diagnosis. Other etiologic diagnosee
given nere toxic and imne rediated. A substanrial nr-der of conference
participants listed cytmgalovirr.E or adsrovirus as the probable etiolqry. Herpes B virus wae listed by a few as a possible etiologic age'nt. Intranuclear
inciusion bodies were observed by all in Ehe nuclei of the convoluted tubular
epichelitm both in intact srd necrotic cells. Discussidr of the SV40virus
included its previously staEd nonpathogenicicy in natural hoets-(1), stch as rhesus
and African Gieen t'tnklys; tlre contain6tian oi early polio vaccires prepared fro
kidney tissue cultures of these anirnnls; and evidence of early vertical cransmission
(3). Tumrs induced by the virus include fibrosarcmas in suckling bmsters
inoculated subcutaneowly; ependymas in srrkling hannstersinoculaced
inrracerebrally and in ni:wboin dst@ys inoculateil subcutaneously; md other
malignancies srrch as anaplastic sarcoslas, lyrytrms and osceogenic sarcmas in
3-weEk-old hrm<rters inoculated intravenousfy (L) .
UniversicY of
DeParEtrEntof PachologY, Sehool of I'bdicine,
MD 2L20L'
ConEribuEor:
uaryffil,-E-fffiore,
References:
of tabor
Patbo
et al. : Viral diseases (S\t40)' In The
& T' C. Jones,
Anirnals, Ed. bY K. Benirschk€, F. I'1. Garnner
ffiE3.
o.
;DaTZ5'L326-
i n t e r s t i t i t a l (to be
2. Sheffield, W. D. et a l . : S i m i a r r v i r u s 4 0 a s s o c i a t e d f aJ.t a lInfect.
Dis.
*t*"y.
rils*
a
in
necrosis
pnetmnia g"d^t?oPftYlYf.T
in occober-issy)'
fiuuiiitua
j. Kaschula, V. R., Van Deltgn' A'
of wild Vervet rcnkeYs
J. Soutn Africa Vet. Assoc'
Sre infectious diseases
in Souttr Africa-
thecus aethi
Case II - cA5-f97 - (AFI'Pf755476)'
u"U6ffiirc
ail;i;"
A
inese haster died at 992 daYs of age'
greatest
its
at
nodular u6ss reasuring about I crn dianreter
srnrounaedthe uterus andcoron'
Endmtrial adenocarcincma'
high incidence in 'aged
;-;;G;i;;iv
"ie1'rcnr chrms*E
nuber (21F22) and
chinese hmsrers. Ttris higfr io"iiil"!=t*il
experirental studies of endctrial
smal1 size make theur suitabfe subj;;a;'for
cancer.
Cqrtributor's
Dipenosis & C@nlqi
*
'cincma,
Attendees'
H#l3?'"ollffi::'
uterug, hmster.
i.ttTil*.' i;-a*&*ti"i"a"
iate additional
was9qapPropr
itrlu;:
incrude
.,:..ffi:iffi:
i:.lrryH;
li:"::ir'3
i
ffi1
I
i*.:f
*11,*
it"
ffi
l;:
ffi*# J;;';;;;'*t ryi*l-gl**i::?:5.:
::*ill::**":'ffi:
arso
adenocarcincma
i:i:#3"T:'I'?"t$l'ffieefffi"l?i:
updifier for this neoplasm.an "at"i'"qtr*u1.u311*^::"t!r}""lnnigr::
#:HlHlqilii.ii'ff;il;;-'"uuii!
ln:T:$^*:*triar
iariousbreeds(3).
of
Institute'
cqttributor: tovelace Inhalation ToxicoloSl Research
Albqiuarqtre,ffi- 87If5.
References:
re-niffi',
S: A:-?nd-PIgok",
' L977'
iathoi. L4: M9-462,
ver.
'--
A. L-:
Sportaneous lesions
P' O' Box5890'
in Chinese hrcters'
in
rwy^ry
endrctriarneopras
efru(lELlrcrr
-splncaneous
.sgSltaneous
1980'
L209-L?L4'
and Brooks, A. !..1;
Bror.msteio, D. G. ^::d'i,i*t9.^1'.1:
2-. ;lhi:i#;.T:
.gid'chi""*
ttilcEit.
JtcI 64:
3.
,spolr.Ta:r^T*ttl::^"d?T*:itr
li."Ef ot
g::-l;-H:**;"1ti#:
il;iffilT^ffi -e..i
,"ootbl"o?;
rr"er;?;"SEl*
ative PathologY, AEIP,
try
N. andvurHaanr,
Baba,
L972.
lesions in a colany of Chinese
4. Ward,B. C. and l6ore, W.: Spontaneous
1969'
hffrcEers.
Lab.
Anim. Care 19: 5L6-52L,
- 2 -
Case III - 7L537 - (AFIP L7L2658).
f several psittacine birds in a pet shop wtTich
- nis
died-fdlfotring a brief illness markedby profuse green hratery diarrhea. These birdc
becaue sick sre week after the arrival of a shipment of dcsEstically-raised
cockatiels and parakeets.
Contributor's Diagnosis & Cqrrents:
th associated herpesviral inclusiqr bodies
(Pacheco'sDisease).
Attendees' Diapnosis & Cmnts:
, liver, Cockatoo.
Eciologic Diagnosis: Viral hepatitis.
Etiolqgy: Herpes virus.
Connpnts: Althotgh ssrp attendees preferred a mrphologic diagmsis of
necrotizirg hepatitis, the cqrsensus was that the primry lesim was necrosis and
rpt inflffnnntion.
Other etiologic agents listed differeirtially
were adenorrirus and
intranuclear
The
eosinophilic
irrclusians
t€re
cctsidered
to be
P,araElJrr(o\tirus.
characteristic of herpesviruses, hcnoevei,the diffuse distributiqr of the lesion
coapared to ttre mrltifocal nature of rcst herpetic lesiars in tte liver of ottrer
speciee _wasconsidered wtusual by scre. A miilimal inflrnnatory cmpcrent ms
reported-by several atcendees. Fixatives which nay b used toenhairce the
intranuclear inclusiqrs such as Bouin's and Zenkeris rderemrtiqred.
Contributor:- Deparmrentof Veterinary Pathology, College of Veterinary
i'bdiETnfIeiF-A6+{ Universiry, College Starion, t{' tlW."
References:
r., Hanley, J. E. & Gaskin, J. M.: psittacirrc herpesvirus
- 1.@-or;9,
infectiqr resdling
Pacheco'sParrot Disease. J. Infect. Dis. lj}l: !j90-396, L975.
Sirysoo,
C.
J. E.: Pacheco'sParrot Disease of psittacine
-2.
!,
birds. Avian Dis. 21:"4-4ll"y,
209-2L9, L977.
Case IV - 27469 - (AFIP U149f8).
d canine was presented on 25 Jan 79 because of
vmriting-over the previous 3 days. It respondeil to syuptsnntic trearrpnc.
Or
30 Apt 79 it was returned after constant vc'nitiog durini the previous week. It was
esraciated,-very depressed, md was markedly dehydrated. Bfooa urea nitrOgen levels
remained elevated despite intraverpus fluid adninistration
ard atteryts aE
peritoneal dialysis.
The owner reqr.rested euthanasia. Necropsy dane-by the
clinician
revealed pale firm kidneys and no other abnormalici.es.
Laboratory Results:
Darem
27 Jan 79
30 Apr 79
L May 79
4
4
5
8
7
9
l,lBc
BIJN
15,000(84%Ses)
5 , 7 0 0(83%See)
u6
l9
110
- 3 -
K+
143
143
3.5
3.7
sT
35
20
GLU
93
75
contribucor's
Diagnosis & cmencs:
Renal amyloidosis.
deposics
sefunenral.co rylglrerttiar
scained
red
"Lu.r"
dmeo
of
Eiainatiwr
material.
of hyaline hongeneously-acidopnific
birefringence of Ehe materiar
sections using crossed Nicol prisrni-r.v.rra-g..""
consistent w-ith-qyloid.
glrerIli
1..1..1^^ :interstitital
plasma
ofc tubules,
"iiti"edditiqral renal changes irnrJJld-a.go*."tiar
nineralized
of
and aggregates
cell infitrraresl-;;d-i"ri"iuu-Jrai-proieTnaceor"-"."t"
debris.
Attendees' Di
, glrerulat,
breed canine.
Etiologic Diagnosis:
eiiofftr
diffuse'
severe, kidney, mixed
Auyloidosis'
Sffitareors dnTtoiaosisor securdaryayloiloeis.
Attendees unsrimusiy-agreed with thi cqrtributor's diagnosis.and
crent:
qt were relatlve anemia and hypoproteinenia
rauori6ri-i""urt"
cm=nts.
felt to be present in
"*6"tEa
a.tya."ti*.
maskedby rhe r.poii'"a-*-.-["d
I lerrogenia^was
in sertm ctremisrries
chcrges
;iit
dil;*;-;'logt
the ra6e*t"g"ii-rh"
$g anenia. and laper marked elevatims
orl !{ere iniEial lolr normal"Na+ and K+ levels,
cmrted
in renal
of cqliiating.thrdosis
in EIIN. Attendees discussed tte-ass;i.d*
hnoerfibrirpto be dr.reto hoc6ncentraiian,
,*,i"n i"-po"irrarcd
ArGi;."iJ,
f*6;;-tt$*ttte.t.[rpercoagulable
*d:"i;t-frmtiTg
genemia, typ"rs,r;;ril"fii;;
of anylirid depositiqrs in various
im.fiogenicifo
(il."Tt;
of
fi"t
!i.r.
6p";;"r
ieveral cases oi ayloidosis in nasal
tissLes was discussed.- One attendee-i"fr.ea
of horses-"*" rt-New Boltcr Cenler, llniver sity of^Pe.nr,rsylvania.
*""""
oi rhi. di""ase reoains urnertaiir. -Prinary gmyloidosi? t* in
Ttre pattogeo"i"
huans, dogE ana nice. It is associated with nrltiple myeloa
-G-d"ry'*yroiaosis"is
U""n-i"di;"d"il
csrsiaered trpre corrmn in animals 4d is thotgltt to
*,r:
including inflsrlnrntory and neoplastic
be associated wich chronic diseas;-siri""
prreaTl aTyfoiao:i? are AL or ]rgpt
qith
Serumproteing ass;iarcd
cqrditions.
chains, ard thos;a5ili;"d
related
to imnogldulins.
"i*t-J**a"ry
Daity injecticrs
lmytoiiosib are SAA!i9!!iql:l
of casein in mice causes chrqrrc
oyloidosis after 22-26weeks. Recentlv
redistributei t9 the-kidney frgm the-primry-sites
iiu"r- in anrnqrs-receivigg daily injecticrs of casein for 21 days
i; ah; spleen
"oa
p.riods of 2 to 6-mrth-s. A sinilar canditiqr also has
and ttren aiscaniin*a-i"ibeen reported in rabbits (3).
"icri."i.-".1*frEi;;;-;""iri"'il;;;i
ir was observedJf,;t*yfoia-"."
Contributor:
USAIRIID, Pathology Divisicn, Fort Detrick, lD
2L7OL'
References:
1958'
f.-Tg6rne, C. A. er a1.: Renal myloidosis in the dog. JAVIA 153: 668-669,
renal ryloidosis
2. Slauson, D. O. & Gribble, p. n.i Thrmbosis cqlicating
1971'
in dogs. Vet. Pathol. 8z 352-363,
-.
An. J'
3. Shirah@a, T. & Cohen,A. S.: Redistribution of aryloid deposits-
1980.
Path.99: 539-550,
Urinary protein exctetion and imnopathologic
et al.:
4. DiBarrokl'S:-i:
Ll7: 73-77, 1980'
diseasl-' JAVt'lA
findings-in dogs'with glmrular
(2 parts)' N' Erg' J'
5. Glerure", Cl-Cl,'-Rtytoia deposits ?d^*yfoidosis
lbd. 302z L283-L292,nd 3022 833-843, L9E0'
TI}OIT{Y P. O,NEILL
CaDtain, USAF, RSC(VC)
Reiistry of VeterinarY Parhologl
of Veterinary Pathologl
Deiartmrt
- 4 -
Histories
AEIP l,.IedresdaySlide Confererne - No. 4
8 October 1980
CaseI-23L97relrctgrt
P L6L9627
to rcve, and
C a s eI I , - 7 4 & -
the qinaf was emaciated,
ifiil#pig.
cLinically,
of dried, yellow nasel dischargea ecait-@rrrt
l-fnitially
tude" in ttre ternlnal
noted etaggering in paetr-re.
stage.
Ttre anirnal
Case III' - 648T - (AffiP L755477r.
r pr€s€nted to clinic rcak std depressed'
pale mrous dranes,
lcterirg,
revlaled
exaiiaticr
Physical
-detrydratict,
No
60/nin., bloody trrire.
pulse
resplraticr
156/nin.
104oF,
tefoeratrrre
No knffn exPosure to toxic
evilerre of external blood loag other tirsr tpaturia.
but died
and antibiotics,
electrolfes
naterfate.
Treated wlttr cortlcosteroids,
3 days later.
Labora
Hct
0.76
t5'
5.0
Hb
Platelers
X 103/cm
-5u5-
3.5
U
Tm
IRrc
zzjw
Spherocytoeis- AnisocYtosis +3
$Pf 700lu/d,
clin. chen. Total Protein'7 .6 &7", IIIN 50 ddi;
Bands
!{BClcrm --:lr
-rr@'
15,300
CaeeIV - L9L7L2-7I3- GFIP 1713082
an a grouP-of. 20 ltrscovy duckshad died
y rePorted was
bS
wirhln the paet 3 iaye. It crasnoted that oEher-drrckswere qr tq p.*P",
tlrere had nirt beena-problen-in the-other_9*!.". Jt yl".reported.tbat.ah,t$,
30%
faces of ttre birds gol u""V dark, alrcet black. Ttre birds irtopped eatgg-bug {id
drink lrater and thd died ;rithln 2 to 3 daye. It ms noted that sc birds had
died in 24 trorrs. ltre birds had mt been iedicated or vaccinated. The diet
cqrelgted of corn, bread, Lettuce and chicken nastt f9r the Fby birds.. tlorsing was
in the woods with-a eheller with 1 glde of the area boarded by a creek. It was
Additiaurl
noted tbat there were ceoent srd plastic pols in the area as rell.
slncfrrpns, a
particular
sufmissiqr-of
hietory indicated thet by 5 daye ifter
.these
qr
died.
preqlse
had
ttre
of the l,irscovy ducfg
majorily
-"-r'ffiioUiofogfc
Pasteirrella mrltocida was recovered fro the ltng,
exsfrnatlon:
livei, lianey &d intestire eu
TII.OITIYP. O'NEILL
Carptain, USAF,BSC0rc)
Re;,istry of VeterinarY Pathologl
Deparffitt of VeterinarY Pathologl
Results
AFIP WednesdaySlide Conference - No. 4
8 october 1980
CaseI - 23L97(enP L6L9827).
the aninnl was
Tistofy: -Tissue frm a female guinea pig. clinically,
eoaciaEed, reluctant to rcve, irrd had a scant mrnt of dried, yellor nasal
discharge.
C,ontributor rs
is ! Crents:
Branchopneuwria, rrultifocal,
subacute,
severe, gu
Ccrrmnts: A _B_Iqm:H-opps
stain revealed nurErous errcapsulated Gra negative
bacterial rods. Klebsiella pneuwriae was cultured frm ttre tur1g.
Attendees' Diapnosis & Cmrts:
eu@tia, sr.rpprrative, subacute, mrltifocal to
..^diffuse,_ severe, lung, guinea pig.
Etiologic Diagnosis: Bacterial pner-mria.
. -ntiolg8lt- l!!t coqatible with i Gra negaLive bacterial rod. A differential
included Bordetella sL, Xlebsiella sp., PastErrreUa sp., and pseudmras sp.
:
cmrFlfn:tiltor6!'f6'IT-eatffisffi
r"r""affi
predoinant neutrophilic reepcrse,- _the clear zones rpted surounaing *rit of tbe
bacteria, and che nrrerous and aiffi.rse vacuoleg or snall clear spads bottr wittrin
--ft.
foci of prerrcnia and thickened septal structures surro*aine-"f|ar
alvJii.
urcderator crented
he had observed sinilar vacuolization in"tissue sectiqg of
PJgJ.dyi$ frn'! this and other causes and felt rtris ctrarg--tra" a,n to
?!!e!_qyilf
edolt.
Ttre origin of the fat correlates wittr a correspddirq fattv
-fac-droplet
river often seen ln eick aqd dying guinea pi&e, and is apparently rilated-to
anorexia and emaciatiqr. the conEents of ttEse vacuoles'trotrfa hive been roved
during processing.. frie pronourced infoEing of the brorrchfobr epitnefirm *"-postulated -to be due to cqrtractiog gf the Fnofnent brancblolar il.rscub*"-upct
conEact with Ehe fixative.
Bacterial agernti respcrsible for pner.mrias in guifu
pigs were discussed.
Contributor: USAI&IID, Divisiqr of Patholory, Fort lbtrick,
MD.
Refererres:
r-ffidE,_R.
F. , lmg, G: G., Abeles,p. G., cancrico, F. B. , Eherr, M. R.,
and Ponsrda, l'[. D-: .Pathoggnggis
of respiratory Kiebsie[a Freurcniae infdtiqr
in rats: Bacteriolog-ical and histotogicai findiirgs and retabilic
alteratians.
Infecc. & Immol. 15: 586-593
, Lg77:
2,. Kunz, L. L. ag{ llglroqr. G. M.: Diseasesof rhe laborarory guinea pig.
r eveterinary scope, pp L2-20,
L97L,rhe upjotrnco., Kalanezoo,ttich'ig;r.
(sP=rZl?99rli
c""" rr - 7a6a==colic.
History:
me ffi6"ad
rnitially noted staggering in pasttrre'
Horse hao srgns ot'
a "dr-my acEitude" in the terminal stage.
l,aboratory Results:- No laboratory work was pe:fotueg:
S",practicing
tiss,.res to the Gboratory. .There
r.."6ri"- ii*J
ffir""tt-*J suE
veterlrurrlan
tto significant findings in bacteriology'
r."uiiJ.oa
were negative viius isolati*
Crotalaria Poisoninq' .
Diagnosis & Crentsi
Contributor'e
't
itrca plants the an-iml had been eatirg'
Crents:
*ictr weie iaentifild ae Crotalarla spectablis'
Attendees'Di
c Dlagnosls:
Stion and necrosis, cenErilobular,
ctrronlci rulEifocafto diffuse, severe, liver'
Ccmenrs: nt;-*.ph;fogic'ai.gqogil-otae
lSreed ragn aft1""*
subacute to
discussion'
coinren!-Td-?"9i1ryi?:f,
Sm attendeesfelt thlre wis a tiTa-i"if^"6rv ls"'.irted qr a nird variatiur in
uoJt-"ir"oa"es
hepariris *"" -ii-d;r"prili"l.
in periportal geag-of ttre hepatic,
size of ttre hepatocytes, prrmrrly-ttpse
on €m occasianal cell wtricb appeared to be
lobules. A few atEendees cmrted
sd necrosis- hrd nild
Ce"ir iloU.Gti"to-";ii;
tundergoing t"g"q"t"i6:Ttrere was considerable
vacuolizatiqr of the hepato"yt"t n"J *porE"A by all.
wtrich have had sc
etiolog.'
conEroversy over 6; ;$;iei
Pltli:ipants
felt that this wasan
ffirotizidinl akaroids
experiencereviarili i;5i*"-d*-F
blcrnrmg.cge andcqrsideredthe lesiqls mre
naturally
a
urusual presentaiicl of
A nr-der of features
consistent wiEhthoseobservedrn-6er,.mhepaEitis of horses.pyrrolizidine
alkaloid
arinarl qi.tn
whichare usualry present in live.-ii*o-iio
portar fibroi-is, extrgoeregglotoxicosis *r"
pointed otrt that the usual case
wis
"6"5ii:-'i#"-i;;*;;-include:
also
rt
fiHi"""iion.
il;;i;;--a-uiil-e;i
rather than acute t6xicity- as. ttre plant is mt
is that of chronic intoxicatiqr
ov"r a itgnq period of tire'
considered palatable in largg.quantitie"
"oo"r*d
toxicosis include-gnna.Eltctql
Serr-menzyme."JJ-ioi-pyrlFiliidfi;-;ikaLoid
(AP). Althougtr ncrspecific, enzyre
phosphatas"
(GcTi-;e"rt"ri.=
Eranspepridase
indicativeor
35'wil ;i ccr ;'5"t06--iu/L-oi--epare consfdered
i:#i!=$"[ilrd
jacobaea in
of
senecio
alkaloids
heoacic das'ge of rhe r)r?e caueJErpyriolizidine
hoises and clttle in thb-Pacific Northwest'
in l|tridt there is
other toxicities discussed were loconeed po-iscrine in horses
poiPogiTq in
mldv
go-!n
gnd
tibrosisl
a rrDnonucr"rt r"Ji6is;-i;aaeiti*-to-pottal
alkaloid
pyrrolizidine
with
cartle and sheep ilt ictr have sm featuies in cmr
ard
cases
acute
in
necrosisdegeneratiqr and
ooisoning such as';enirifobutar
cases'
chrqtic
in
iibrosis and bile duct pioliferatim
5il;;ifi
Etiotogic Diagnosis: Toxic hePatitis'
Eriology: py?r"iiiiai"I""rur5ia-p"i"6ing
or serLmhepatitis (Ttreiler's
Disease of horses).
Conrributor:
SracToTlNffifle,
C. E. Kord Aniqal Disease Laboratory' P. 0. bx 40627,Melrose
TN 37204.
- 2 -
References:
f. .Iu66 ,F-Kennedy: Pathology of hstic
Aninals, Vol. 2, AcademicPress,
L970, pp 2L6-2L9 and pp 22L-224.
2. Craig, A. I'{. et al.:
Serumenz}rtrEtests for pyrrolizidine alkaloid
toxicosis. Arer. Assoc. Veterinary Laboratory Diagnosticians, 2lst Arnual
Proceedings, L978, pp 161-178.
Case III
- 648T -
L755477
37'5 )rears old, preeented to clinic neak and
sd:--"hysical
exalnation revealed icterus, pale mrcous nrrrhran€sr
aticr, teryerature = l04oF, pulse 156/nin. respiraticr 60/min., bloody
urine. ltro eviderrce of external blood loss other than henaturia. No knarlr e:oosure
to toxic materiale. Treated wittr corticosteroids, electrolytes and ancibiotiis,
but died 3 days later.
Hct
tt:
Clinic afuiss.
Hb
v2
Platelets
X 103/cm
-ur-
NRBC
t{BClcm Bands
-rrJm
-T
0.76
5.0
3.5
U
221L00 15,300
Spherocytosis - Anisocytosis +3
Clin. Chem.Total Prorein 7 .6 tr€:l",HrI 50 q/dl;
SGpI 700lu/d.
30%
Contributorrs Diagnosis & C@nts:
A diagrnsis of autoinrrurnehsrnlytic an€mia
was
heofrclogic findirgs.
In additiqr to
the Eubular necrosis and hemaglobinuria the dog also had centiilobular necrosis of
the liver md nild nuuron erythroid hyperplasia.
A t t e n d e e s'D
ie & CoEEEnts:
atiqr and necrosis, tubular, acute, mrltifocal
to se'\rere, with rultifocal hoglobin
casts, severe, kidney.
Etiologic Diagnosie: Biliary ed hmglobinuric nephrosis.
Etiology:
Autoi@ne HmLytic Disease.
Crents:
Tbere was general agreerent with the cantributore diagnosis and
cqnEnts. Other mrphologic diagrnees given by a far participants were
glmrulonephritis
and glmrulonephrosis;
trcnever, after s.np discussiql these
were viened as tnsubstantiated without appropriate thin sections and special
scains. Gls'Erular lesions are not a reporEed feature of this conditiixr in dogs,
but are seen ln systemic,luprs eryth€natosus h@lytic
anemia of dogs. Ttte
pathogeresis of the tubular'lesions in this case was discussed. Hpoxia was
favored over a dlrect toxic effect of ttre troglobin
depositicr, as-a plausable
explanatian. Ttre left shift in the neutrophilic series was arrnrpn6sdcr ard is
reported in this cqrditiqr.
The underlying cause was rpt ascertained, but nay b
relaced to tlre bone marror hyperplasia and release of blood cells.
Cartributor:
lpvelace Inhalation Toxicology ResearchInstitute,
5890llilI6ffirque, lwt 87[5 .
- 3 -
P. O. Box
ffifi'.Jean:Autoimir.l|ltj|.9TorI:1",.ii;ease'an
dothercausesofimne-JAVI"A
r e d i,2.
a t e d a n e m i a : A n o v e r v i e w " A A l t ' j r L 3 z 4 ' 3 1 - 4 " L ' L 9 7L76z
7 ' 970-974, L980'
Harvey, J. W:i--C"r,ir,u t.trrrycii "rr"*i,",
case IV - 19Ur2-7U - (AFIP Y8082) '
-T-eT.ieEory
a group.of.20 r4usco_vy^d'crshad died
reporred ,r"" *.ffiirqn
lfere qr ttre premise, but
-IE
wiuhin rhe pasE 3 days. -|t wgs'*t"a-ffr,at ofher'ducks
was-reported tirat ttre red
drrk'.
there had not been a problem-i.r. In"-,ither
birds -itopped earing bur did
The
d.;rl-ar,msi .brack. .
faces of the bifi; ;"r;;y
days.-. LE was nored thil sc birds had
drink warer and rhet died wirhi,i'z-l,i-i
or vaccinated' The diet
died in 24 hours]- fte-Uiras na'i *i-U"ot-it"ai':aced
Lhe baby birds. rlo'sing was
for
r'ash
consisted of corn, bread, letturie and chicken area boarded by a creek. rE was
wittr-r-side of the
in the woodswirii'"-"t"ii",
the area as well' AddiCimal
*d pf""ti.-p*it,in
cetrEnt
noEedthat there were
specirens'a
of -rheseparricular
hisrory indicated-Itir-uy 5-d"# ;il;;-;"hission
6n chepremisehrd died.
d;t-aucr.s
;j;;i'ry-oi-it"
recoveredfro ttre ltng'
g*gla*s
r"ticrobiorogi;exdinatlon:- tlfgg+?,
livei,
kidney Ind intestine
suh,[EEe,I for culttr'e.
ContriPutoj's Diagnosis& Cfimrnts:
Gross r,"rr*ffit:fre'r"ralr
the head*." u"ry-"y"*ti".
::".il:T""T;,EI;'.:;";;'r#";i
-o'd body cqrditicr ard the skin on
rnternar.iesionsrlctu!e!-ryv-f,:ih:*-l3i:|:g:?."
pericarditis with
;nd river.. Th.'" wasa marked
in rhe t;;r;:- ni. spreeorst{ere very.soft ind trorrhagic
apparenr focar
";;;;;i;
f;-t *te-cui surface. the livers hbd a very mttlednecrosis
airi Ufooa .*ua.j-Ir;;it
were discrete areas of
appearancewigh apparent areas of-neciosis. There
attached to the surface. Ttere
mesrbrane
in rhe incesrlne-5iun-li'ri6ii"oL;aia
had been dead the
of ttt" guc ip the bird which
was free blood i"--*,"-"f*I,*;
of the-proventriculus ard
ttoo.td{e.];9
lorgest. There'"."
*. 3ry"iiorr
It was
left hm'
esophasus in me 6ira-ntt,i"r, traa aiea before-the ootner bad
particurar
this
and
since-ctt" o*r.r.r had left hm
;.rffi$-.tur-i-a,*t1"d-ai"a
blood in the'h-ren of ttre
*iit-rtte-excepti*-oi-i".e
i."i*"
biid had u.ry
jumcEian of che proventriculus and esoptugtts.
"i*1",
fiemo[;4"-;i-ihe
iri-*a-4re
LaboratorYFindings:
{*Ares''
in-1te
Virologic ex@inaEim: Virus isolation atteryts*are in center
Prog-res:
rolrain
Disease
nnimir
rhe
Nationat
ar
and
ai-wu
Eecrion
ui."iotinot
has
Virr'rs
WhenEheseresul;s-"." oUl"i."a " J"pplrenEal.rePort Yitl Educrs'
"Pt'
Ehe
ot
Eisstres
fro ttre
-pasteuretla
tleen isofated at this point in tinp
mrlcocida was recovered frcm tlre lung'
I,ticrobiologic Examination:
-On for culgure.
livei, kidney Ind intestine suhitted
*i"t-o9copig exminatiqr there nere fairly
[,,"rination:
Histopathofoii"
dact of bocir birds. There was desqr.raati-ar of
severe lesions if,-inl'i"G;i;i
hemrrhage inEo the suhrcosa and tunica m'rscularis in sm
*rt-"s
epiEhelir-m
tissue within Ehe submrcosaof the intestire
"" ,""-*".osis
of irutoia
areas. There
an
in sm locations. In addition, Lhere r,'reremanybacterial colqries-gererally
a
nell as in
the mrcosat surfacer-or-itt" ulcerated rucosa ol the inEesEine as
cr the
necrosis
and
desquamation
was
visceial organs. There
;;rb".-;i-ctre
4 -
epithelirm of the trachea in Ehe bird which had been dead the lorgest. There were
ni.uerous bacterial colonies qr ttre u.rcosal surface of the trachea in this
particular bird. Ttrere was severe hyperesda and focal horrhage in the lug
without mrch indicatitrr of inflenmqtory infiltraEe into the luqg. There was fairly
marked hmrrbage in the ryocardir.m extending in sre areaa througttout che total
thickness of the nyocardir-u frm rhe pericardir-m to the endocardium. In eeveral
sectiqrs of posterior intestine fro the birds there was rnaegive horrtrage into
the suhrcosa with necroeis of lyryhoid nodules within ihs srr[rntcoga in these szn
sectiqrs of intestine as rreIl as s@ of those anterior to this locatiqr.
Ttrere
waa rpcro{ris of ruch of the epitheLitm even dqn into Clre crypts wtrile the
interstitium surrounding ttre crypt epithelir.u was relatively normal. There rere
often large bacterial colqriee within the areas of horrage and necrotic tissue.
0n enainatiqr of the liver there were areas of necrosis with intranr.rclear
inclusicr bodies in varying stages of developrt
in nr.rerous hepatic cells within
and at the edges of the areas of necrosis. In additim, there rere areag of
eosinophilic globules surromded by halos in ttre cytoplasn of s@ of the
degeierating hepatocytes. Similar cytoplasuic ctrages had been mted in the
sectiqts of intestine exa'qined. There appeared to be disruptian of the endothelial
lining of the sinusoids in che liver at s@ locatiqrs with trorrhage into ttre
surrourding hepatic parenctyma. There was no indicatiqr of infLmatory
ctwlge in
the liver and bacterial colonies were not notd in the liver of the drck wtrich had
recently died. There was ccmplete disappeararce of fyryhoid tissue within tbe
spleen. There ttere areas of hemrrhage and variable munts of corgestiqr in
nearly all the other Eissues exained.
Conclusions: The history, gross and microscopic lesims ce cqrsistot wittr a
provisional diagnosis of duck plague. In additiqr, it was felt that the 2 birds
submitted to the laboratory had a cqlicatirrg
terninal Pasteurell mrltocida
infection or fonl cholera. (Virus isolation att€rycs as well as other laboratory
rcthode of donsEration of viral agents are in prqpess and will be reported at a
later date as ttre results becm available.)
Supplmncal Report:
Virologic exaurinatiqr: An oral report has been received frm the Naticral
Animal Disease Certer, AuEs, Iowa, indicating that the virus of dr.rckplagrr, c
IXrck Virus Enteritis, was isolated frcm the tissues submitted for exainatiqr.
Corrclusiqrs: Ttris finding confirms our previous diagnosis of drrk plague.
Attendeeer Di
lbrphologic Diagnoses: I) Degeneration'and
and necrosis, acute, mrltifocal to
diffuse, severe, with eosinophilic intranuclear inclusion bodies, liver, Mlscovy
Mlsco'
duck, avian.
2) Enteritis,
necrotizi4g,
acute to subacute, diffuse, mderate tb
sgvere, so,?11inEestine:
3) Congestion, acute, diffuse, severe, spleen.
4) hreuuria,
hmrrhagic,
peracute to acute, mrltifocal,
mderace, lung.
Etiologic Diagnosis: Duck Viral Enceritis (D[/E), with seccrdary Pasteurella
pnermnia.
EEiology: Herpesvirus.
Crents:
Tte above diagnoses were reached afrer src discussiql of the
lesions.
Ttre tissues_present in the suhritted slides varied as to *trich orgars
$rere Present. llcst slides contained liver and mall intestine.
The distrifutiqr
std severity of lesians in the different sectiqu varied fro nrltifocal
to diffuse
and fro mderate to severe. A nr-der of aEtendees corented on a mderate
- 5 -
lesiqts
postrrprEemchar€,e present ln cheir slides and felc this nesked sre of tbeatd
intestinal
the
within
- apparemcOacierial orrergronth
ln
fii;;;;-reb-d[r
in
p,;h-;t
tissLres. Sd;-p"rEicipanrs did noE feel a true pner"wria was present
Tbe
horrhag-e
and
corgesEion
Preg:nt..
the sectiqrs of irtg"-i"t"A;""d'there-!ras
csrsidered
and
all
by
obeeru"q
wer6
bodies
iiclusiqr
i"rr""ftkar
J6"i"rptrifi"
irrciusims. A fen attendees-reported-9eeiE similar
h;;F-viral
characteristic
"i
stotrgtred and necrotic intestinal lining -".11". Cgtt
irrclusions in s@-oi'ttr.
A Ioose
qt the
congestlcr of the 9P19en-md_ a fyryhoid depletim'
cqrgidered
but
wis'reporrca
"iver"
"""-*a"
scnr[iI[.*s"i5.i
rericutar
vieo oF A"-c*rg"etian-ffi
rprmal ln "pp"""*ci-""o*E
species susceptabiircy 4}rg
eifectiveness €il)r€ birds.
Contrlbutor:
P. o:ffiWffi
c$ree, Dislussidt irrluded
pc@rtem"t.attrs
Anserifoines and vaccinatiqr
(im.nizatim)
Anirral Healttr & Disease Laboratory, l.lichigan State llniversity,
Lansing,MI 48909.
References:
L.: Grossandhistopaltt"lggig^$Tqgl of drck plagrre(urt
ffitz,
virus'ent"iiii">.' An. J. Vet. Ree.32 Q)-: 275-2n, L97L:
in-ttre Bureaof Fabricius,
of 4,*t plqgygZ. proctor,'S.-.1.:--Pathogenesis
(4\:
427-41fr-L97-6'.
An. J. VeE.Res.J7
- '-,.' Aa splien. -l"tiv"
th)'mt;,
inmrnizatim-of!{hite Pekindrrckslgainst DrckVirus
Torh,'T: il:,
and
gnreriris <O,rctPiague) lrith rcdified live virus vaccine: Serologic
responsEo'f Ureedera,rcts. An. J. Vet. Res. 32 (t)i 75;Q1, 1971'
i-umr.nmlogic
of Poultry, 7th Ed., 1978,pp 62L-632.
4. teiUovitz, L., Dieeases
TI}OIHY P. O'NEILL
caprain, usAF, B$(\rc)
neiistry of VeterinarY Pathologl
Departdnt of Veterinary Pathologl
- 6 -
Histories
AmP l,Ie&teeday Slide Cqrference - No. 5
15 October 1980
CaseI - L76750
m" io,5a- dorr g; aug il,
after beirg ieolated-fro
f *fgl was fornd aea.aF the ?dtfblt-. .A e€catd btrd
i978, iovea fro the srhlbit, and ee@d to recoYer
the grq4.
L667702
i ;"i;ili"';;fii*;**"'
aborrt three
"ii.s, years old, preeented to
observedto
itre protuUeraircee-were
r*d"Fi
the bmee.
trer).
Eilnflp
CaeeIV - 4898-K- (AnP L672455).
oi it€ uoav.-CI
-f,o.="
be a r*rite, fitn tlsgtr, eurrorndlrg
io-*t
T\p days prtor to death tte bird ces
wtrlte droPlngs.
. Ttris sp-rirgmte<i !^rt m eggpe59d!rc{.
in-dre poeteri6i abdmr ailJacent to cloaca. thor4ght to be
Several Lqe'fe1E
Rm\ted forcibly by speezing
ircacted eecs. verifled qr'radiologic exainatiqr.
later.
12
hor-is
died
thioqgh cI&ci.
Snake
TTIfiTff P. O'TEII.L
Captain, lrsAf,', BS(\,C)
ne!,tetry of Veterinary Pathologl
Deparrnqt of Veterinary PathologT
Results
Slide Conference - No. 5
AFIP Wednesday
15 october 1980
Case I - L76750(AFIP f68ff07).
A
aco (band #459) was found dead in the exhibit.
to
secd
and
secqrd blrcl was for-nrddown-on Aug 22, 1,9lg, r@ved frm the exhibit,
recover after being isolated frcm the grouP.
Contributot Diaprrosis & Crents:
on arrival and in fair condition. External
lesions - ttre rieht lee had broken bqres and skin over che leg was sliglt1y
Internal lesiqrs - Ttre liver was
hean'rrhagic and discol6red (purplish-black).
that varied frm 0.5 to I m in disrpter whidt
souewhat"enlargedwith mrltiilfe'foci
was markqdly err-rfarg{, being 3..to,5.
spleen
yellowish"wtrite
in
Ttp
coloi. .
were
wtritish foci to those described in
similar
contafnedtiuEs normal silze, srd also
wtrich lrere l*ritish in color
the
intestine
l,lany nodules were seen along
rhe liver.
necrosis
caseous
and quite hard.- There were'sosn aEeasof
Present in the trDuth.
Laboratory Findings: I'licrobiologic Exarrinatiqr: The Ltnrg, liver, ad kidney
Streptococcus sP. and-Klebsiella
contained heavy gromh of beta-hmlytic
Streptococcus sP.
coli.
The beta-hmlytic
pner-mniae and-a-mderate grorEh of E.
gentanicin,
cephalofin]l?-6'loraphenicol,
iras sensitive to opicillit,
-lircoycin,
polymyxin B, and tetracycline and resistant
nemrycin, nitrofurairtoin, penicillin,
The Klebsiella !{as sensitvie to cephalothin,
to sirtfailirethoxine and tribrissen.
nitrofurantoin, polyrnyxit B, tribrissen, and
gentarycin,
necrnycin,
chloranphenicol,
lincurycin, penicillin, and
tetracycline srd iesistant to apicillin,
sulfadirethoxine.
Histopathologic Examinatiqr: The liver had mrltifocal areasi of caseatiqt
necrosis that weie frequently surrounded by large fosy macrophageswith occasional
mrltinucleated giant cells being present. This had a relatively randm
distribution.
f,re spleen had similar-type lesicns but with mre necrosis beirg
present. The similar-type lesions were also seen in the oral mlcosa thaE was
described grossly. Sisdlar-type lesicns ltere also seen in the ventriculus. On
special scain (acid-fast) there were prodigious nu$ers of acid-fast bacilli seen
throug,hout virtually all tissues exmined. This was particularly remarkable change
due to the overr*relming nrders of these organisms.
(This has to be ane of the rcst severe c:tses
Conclusiqr: Avian tuberculosis.
of avian tuberculosis I have ever seen. It is a strorg possibilify that there will
be ocher Losses due to this disease with birds that were in the sflne housing.)
AtEendees'DiaFFoces&CouuFnus:L)Hepacit-ls,granulsntous'mulEifocal'
tousl diffuse, severe' sPleen'
,"u
3i-F;&Jiricufiii.*,"gi""uionuEous, multifocal, rnderate, proventriculus'
[i' Mt;"iiis, suUacuie"tochronic, difiuse, nnderate,skeletal mrscle'
gtiotogic Diagnosis: Gastrointestinal Tuberculosis.
EEiolo[y: ]g6o4cterir-un-9p=ProbaP,tv
L ?Yit-'
on the appare?iE-granulcmatousrespqFe-observed
ccunented
-oirf"r"ntiar
CcamenEs: AEtendees
diagnoses giiln incfided entities such as E- coli
in many Eissues.
or ;ikrei sloragg diseases' ffi'ffif
i"nEr,*tcolis
infecrion (coligrai;i*t;;i-;;i
19161in all
fast stain proviaea aeonnitrared large ntmbers of aeid fast-bacilli
surrounding
cerls
unrltinrrcleated gimq
-oiner'bacterilt
rhe rissues. Th;-ArB-p;imarily
were
secEims
""r""*iini"
ele.rpnts noted in the submitted
the sranuloras.
not in
but
granulmas,
rhe cenrer of several
&3;ffi"^*#"L"Jpiiiri"-"*ci-within
Ehis
that
out
pointed
The rpderator (Dr. Montali, National Zoological Palk)
cells.
cellurar
tLre
that
in
in avian-species
was an arypicar ;;;;"i;;i;;il;b.;culosis
tuberclei were *,:,ry:"::l:rrl1i*
histiocytic-and-typical
prim.rily
i.lls
;;;6";
spindlerubercles cnaracierislicatty c6nsist of tiseous cencers surror.ndedby
and
orgctim,
tbre
seroty?e-of
shapedepithelioid cells. Defenses of the host,
inporEanrroles in tlri disease. Thenecrotic
cb.tsidered to E degenerating, and necrotic
and lack of ab5cess foriatiqr in birds is
ttre-aEsenceof
hisriocytes.
""uii"iion
atCributed Uo ttre .U".""" of proteolytic enA|IIEs in the avian heterophil: -IFf r-3'
by Lglllum intercellulariae seroty?es
cases of avian tuberculosis are-A;A
due to l{. avium occasianally
4-20 usually are nor involved.- mbEffifosis
a;;;iyp."
in a nffibbT-d.captive
observed
been
trr"
p*pl; *d
occurs in irmunoilil;6sJ;d
of
2oo. A !arg9 ynUeS
-these marsupials had caseous
maisupiafs ar rhe ilitimaf
to avian strains' Parrots
addition
rn
.
cdrncn in avian-T.B.
-t; are-tuberqglgglq
r""ioir wkrichalso
A few ParticiPants had
bovis.
I'1.
in*t"i"l
are susceprible - M.
.and
resedled
"j"itiuiiiil-;i-il"-ort""i"*-pi"y
cen6ers within ti," gr#ulwras'neie
our"iu"a ies ions in,if@
tuberculosis but yl"fa"i CgEy!@
acid fast ,r"i1u !ffiil-#t"p"red
birds over 6 rsrths of age.
Contributor:
Ueaiffibn
mici6sEdp'iG'tLy_
equi upor culture' Iryression srears for
lesions of
ar necropsy'fromall granul6matous
Animal Health Diagnostic Laboratory,^College of Veterinary
Srare University, Lansing, ML, 48909.
Reference:
T u b e r c u l o s i s in captive exotic birdsJ., et al.:
Mo-ntdli-.
L976.
(9):
920-927,
JAVMA169
- 2 -
- (AFrP1667702
Case II - 77PNI623D
rqn a
, f@ale, about three years old, presented
fo necroPsy
multiple protuberances in rpst long bones of the body. Ort
necropsy the protuberances were observed to be a nftiEe, finn tissue, surrotnding
che bones.
Contributor Diamosis & Conents:
Multiole Chmdrmratosis.
ectioni of this skeleEal bqre strownarked
periosteal cartilagenous proliferation with soe ossification.
The cortical bqre
shows no evidence of involvwrt
with any pathological process and sesns rnrnally
calcified.
ltre preserrceof this change in-the majority-of the bqres wor.rldbe
consistent with a nutritional deficierrcy with this particular reaction in chis
species or better, a virally induced reaction sirnilir to that of osteopetrosis in
chickens. No evlderrce of either was present in this case.
Attendees' Diagnoses & CwEnts:
drmatous hyperplasia, diffuse, severe, lag
bore, iguana.
Etiologic Diagnosis: Nutritional osreodystrophy.
Etiologl:
Calciu-phosphorus imbalance.Cmnts:
Histologic features of the lesiqr nrhich were discussed included cne
lack of mineralization of the osteoid, the chqrdroid hyperplasia, and the scattered
endochondral ossification.
The differential
diagmeis givin was osteochandrona.
The lesims presen! paralleled- a sinilar cordition that-tras been reportd in green
iguanas (1). The lesions ln this species have been arcribured to cilcitr
phosphorus itrbalance in canjunction with stress. The disease has been seen in
active animals especially. Ttre lesions described were fibrocartilagenous
osteodystr-ophy, parathyroid hyperplasia, and hypocaliemic tetany.
Proliferati*,
Ttre conditigqr appears to be peculiar to Lizzaids-. Dietary urairipulation with 16w
cglc.ryn or high phosphorus diets or cobinatiqls
Ehere of , resuit in the producticr
of this disease in green igFanas. In natural conditions fibrous osteodysirophies
of undetermined cause have-been observed in hatching iguanas, nrnture igiJanas, ad
Geckos.
Contributor:.
rcdiEinffiIE5fana
Ibpartmrt of Veterinary Pathology, School of Veterinary
Srare universiry, BarbmRouge,-LA 70g03.
Reference:
,
ffi,-M.P.andCapen,C.C.:Nutritionalosteodystrophyincaptivegreen
iguarras qgum" jg*rr.).
Virchors Arch B Cell parn 2l: 229-247,-L976.
- 3 -
ilv, and
oirffi#t,û".ur"-lo
Laboratory
il
r-
deaththe
(c1vlg,',",=l)
;,...y.91v:-R::?:,:o
had br<ffEine-ar6ilky whire dropprngs'
Resulcs:
, r
:
i r .r
-^-arirra
r\ironr
tr'l
FA for
.
Direct
were negative.
AeroEIe baccerial' curEures crf-t liver and kidney
negaCive'
was
also
of'kidney
ead-analysis
NewcastleDisease was negative.
due.to wggyP?'.
conrribucor Diagnosj.s& ccrrenrs:- Renal Coccidiosis
of
ffir
ed case oi-r.rrr-coccidiosffi
an
be
to
was cqrsidered
-rE
the Order Gaviforres. lbrphologi"Sily, the coccidian
Eireria gaviae, o. sp'
ne"nd
was
Eireria sp. aisiiili'rr*
Lr*iii-ci"i-t"rir.
Attendeesl
itis,
subacute, unrltifocal,
mild, kidney'
ioon, Gavia iuml
----giioiogic@to
Ccrnrpn
Renal coccidiosis'
gaviae'
EEiologY: Eimeria
inflzrm'tory
lesiqrs included the rnild subacute
ccstrFnts: offi
pervis
and disral
renal
rhe
reacEion and rhe presence of coccil;e" p";Fires withinnecrogis of lne ePitheliLm
.'*; oir rlre
SnE participants
redulla.
"PP.t;;; various stages of the parasiEe
".-o,i"a
r".r"l-p"ii'i".
tubures
lining rhe corlegrirg
"1,
of host respanse to thelr
were present in f"tgE nr-ubers, but Ehere $rasa lack
the organism'and
to the oqtn"teq"i!{ ?-i
aia coilsidereiran incidental
ic was felr rhes;-.*;;;;"iry-.""paihogeric.
ilitd hyoerolasia of EheureEer
Dresence.rni, sfifr;il;;-ffi"ut"io"-"s
i"* artendds corented bn a
iil;ng:--e
parasite in birds
eoichelitm. Eis=ria trulcata is the rpst cmuDnii-F"*a occidian
riPst species'
aia-G consiffi
contributor:
-46 Eli LiIIy & Cqany,
GreenEidfcliTfr
Greenfield Laboratories, P' o' Box 708'
140.
Reference:
R. B": Renal coccidiosis
MonEgore-ry, R. D., l'lovilla, M . N. and Shillinger,
(Gavia
inunr). Avian Diseases2 2 |
Loon
caused by Eirerie gaviae n. sP. in a CarnPn
809-814,-Lq7tr
(
case IV - 4898-K- (I-FIP L572455L
but no eggs
7 eggs.
esss..'this
agor laid 7
,lhis spllng matecllY:^::
@pFlngs
@i'Egrqsqkid
-*
"qiiry^5t:1
r;-lh;-P"st9rTE:3!Ti-;dj3:j::^:"r:l5f?;
-Respved
produceo.
bv
forciblv .:housn'
"".,"'Ji-r*p!.i:Tf ;;.i;qig!;pic-ex"minarion.
[;H-fr""iEi=iEir,*#iiii;i
speezing'through-Eloaca.
Snake died 12 hours later'
Contributor Diagnosis &
inflaumation,
Kidney, snake, gouty toPhi, chronic
and gIqIEru
CorrenE: Ttre lesiors are similar to those seen in snakes given genEacin
Eherapy, but are mre extensive than in those cases.
, necrotizing, chronic, disssdnated, kidney,
snaKe.
Etiologic Diagnosis: Renal goutl toFi.
Defiydration, genq]qycin.theraPy, 9r hieh-Pl9lein.,diet'
itioro[y,
diafgrosis gi"9" above was-reached after sc
Csrencs: Td';;phoioglc
discussion. Oth;; Ai"gno".E given"were clbubr n9{rqsi9, &d glrcrulaephritis' the
of
Chanses in che tissue drich were courrpntoJ on included the segrental naturegiant
fibrosis, lhe presence of mrl-t{nrrleated
;';;;r;;"-ir,t"r"ririral
il;A;
&d the abundanceof birefrirgent urate.
deposits,
ur"t"
tk.
;;iG surroundine
of rhe lesion and the apparently rnaffected
Th"-;;rc;6i-;;ilre
crvsrars oresenf
nephrons were Eonsidered unique in this case. The usual Prese.1tsglcn ln
""ii"tr.i"i
is'a diffuse lesion wtrereininterstitial edm
;5"e;iltar"lio-""ptiorir
l;;iffi;;;
;acrosis and'urare deposition in tubules wtrich in turn result in
fiilAfi;ril;I";
and chronic infilmnatiqr. A few participants speculated on a
iilli]ii.iJ-iiUrosis
i"".t ischenia due to a vascular injury resulting in the lgqi-onpresent'.
Seqr.rentialbiopsies also have.producedsimilar lesions in kidneys ot snakes
Occasional
according to- the mderator.
r.mdergoing
tissr-p but the
the
ol
"*pirio=ni"f-pi*.dures
regicrs
perip6eral
sclerficic-gfcmeruii *"".'noted in the
rh
nofoal.
unaffecced and were ionsidered
rnajority
"pp."r"d
a no*ql findin' :" "i11Tq*-aP-:*:5
sosegtomeruliis considered
,ffu"r"hce'bf
IgP9il,ill_Il9:f '
diagnosis
Ehe
sections,
airil without special-stains on thin
-o{ -q}@rulonePnrrEls
would be rnrsubshntiated. Gouty tophi occur in a nuber of different organs.in.
repriles'with renal disease. Tophi.are observed in the
addition ro rhe-kffi;F-of
-frodt and other-visceral organs; this reflecEs the
heart, pericardii-,
fG,-fiuer,
rhe inabiliry to excr6te the breakdonn products of
totricri ;;ill*
nfriuii""ria
protein catabolism.
Contributor: LaboraEory of Pathology, Sctrool of Veterinary l&dicine,
Philadelphia, PA 19104.
UnivEf5iEy-ffinnsylvania,
References:
FJIIa,eET n. and Hoessle, D.: Visceral gout in captive reptiles.
8 9 7 - 8 9 9 ,L 96 7 .
Jacobsen, E. R.:
JAW$ uI:
relared visceral gout in ttm bold snakes.
Gencarryacin
7l: 361-363,L967.
lnvsec
yrntrli, R. J., Bush,M. and Sueller, J. I'1.: Thg pathologl of nephrotoxicify
of gentmycinin snakes. Vet. Pach.16: i08-1U, L979.
.II}OITIY
P. O,NEILL
()aptain, USAF, BSC(VC)
Registry of Veterinary PathologY
Dr:partrrent of Veterinary Pathologl
Historieq
Slide Con{'erence - No. 6
!{ednesday
AFIP
29 Occober 1980
caseI - Lese-76(nrIP El99!9I.
HisCory: A 4-year-oro orcstic shorcbair cat had digced pupils for 4.treeks
*affit"'yp'i,ui*2week1priortoex*iY.1T:-I:^.::wasanorexic,
ani frad diarrhea. ocular examreveated discrete areas of bilateral
a.uiiiilied
recinitis wittr nild papilledena.
CaseII -
P L7L2745
. tilecropsy findings
before
and mrcotremrrhagic cYscitis.
Case III
- 7153-79Dor
year-
because of
upper canine tooth.
exhibited
included fyelonephritis,
ch-old eilt
h€tr'rturia for 3 or 4 days
ureteritis
horrhagic
L7L270L.
Setter was Presented to Ehe
.5 cm swelling of che gtm aE ttre base of the rigbt
P,adios,raPhicFind ings :
of the naxilla ac the base of the righE canine toottt.
E@
tesioir is surrounded by a uriforn tbin rim of sclerotic bme.
ffre filic
Case IV - I'G 78-430 (AFIP 1667745)'f bocn sexes appeared in
----J-
fror a
"**r"rJbreeder.
tne itiected
Clinically,
dyspnea.
14 days afcer arrival
MorbidiCy was over 507"with uDderaEercrtality'.
mice exfriUiteO exErellE lassitude, ruffled hair, and
TI}OITf{ P. O'NEILL
CapEain, USAF, BSC(VC)
Reiistry of Veterinary Patnology
uefururlnt of Veterinary Pathology
Resulus
AFIP Wednesday Slide Conference- No. 6
2 9 Occober 1980
shorthair cat had dilated pupils for 4 weeks
debilitated and had diarrhea. Ocular examrevealed discrete areas of bilateral
retinitis with mild papilledema.
Laboratory Results:
ToxoplasmaEiter-negative.
Contributor's DiaFrrosis & Cqupncs: D,tycoticrenirgoencephalitis;
Cryp@],
Cdrpnts:
Cryptococcus organisms were fourd in lesions in che eye.
Attendees' Diagnosef Q Qqrspnts: Cryptococcal reningoencephalitis, mrltifocal,
upde
horliair, feline.
Cqinents: the above diagnosis was reached after src discussiqr of the lesiqrs
and host response to the organisms. An etioorphologic diagnosis was preferred on
the basis of the large nusrbersof organisms involved, the destruction of
considerable neuropil, and the corylete lack to minimal inflrnmatory response of
this anirnal. Various uorphologic diagnoses !{ere submitted which included
descriptors srrch as: ncrsLrppuraLive,chronic, granulmatous, subacute, and fungal
ueningoencephalitis. Curents on the lesions included distribupign, and tissue
desEructim. Ttre distribution was felt to be closely associated with the reningeal
extensions into the neurophil via the pial- lined blood vessels. A nuder of the
lesiqrs in the brain substance contained central blood vessels with organisms in
the distended Virchcne-Robbinsspace, or in areas forrerly occupied by brain
tissue. Discussion of other lesiqrs courranly associated with this organism in
md cutaneous lesiqr.
various animals included sinal, ocular, pul-uurary, Eurnrnary
Extension of cryptococcal infection chrough Ehe cribiforn plate to the brain was
discussed. Invasion by the organissrs via the reninges covering the olfactory
nerves seernsnnre plausable than direct extension. Extensiqr to the eyes frm che
Hematogenousspread was cqrsidered
CNSalso was considered ueningeal in origin.
unlikely in this case. Participancs generally agreed with the proposal that this
aninal was not respcrding to the organism due eithel gs inrnrrosuppressim, lack of
anEigenic stimulus of che organism or boch. Concurrent infection with feline
leukemia virus and its clinical manifestaticn of generalized imt-nosuppressian
could account for this. Also the antigenic determinants of the cryptococcal
capsule play a role in the host response. Lesions also occur in inrrr-nologically
privileged sites with little host response srrch as reninges, iris, and joints.
Lesiqrs in oEher animals discussed hrerecryptococcal mastiEis in ccxrs, pulmnry
granulouns in donkeys and horses, aod CNSand pul-uurary lesiqrs in dogs. Clinical
differential
diagnoses for ocular cryptococcal infection in cats include: FeLV,
toxoplasuosis, and FIP. Diagnostic aids in detection of che CNSform should
include exanination of CSFpreparatitrrs stained wirh India ink. Stainirg
characterisEics of the organism in tissue sections in mse cases are as follows:
The cell wall and capsule-stain deeply with urrcicarmine and less intensely with
also stains the cell wall.
PAS. The capsule atso is Alcian blue positive. @1^S
VeCerinary Diagnostic Laboracory, Otegot State University'
Contributor:
CorvEIEs-iF97331.
References:
Intraocular
T.@.A.:
r9r-198, L97L.
cEyPtococcosis in cr^rocats'
JAt/lA 158:
in a dog'
2 . G e l a t t , K . N . e t a I . : Ocular and systerric cryptococcosis
JAVI"IAL62: 370'375 , L973.
A new experi3 . P e r f e c t , J . R . e t a I . : Chronic cryptococ€l urenilgitis:
f980.
L77'L94'
fOi:
Am. J. Pathot.
rental updel in rabbits.
CaseII - 79-8074(ArI!-12r2245)'
a
Hlscory:
gilt exhibite<^ herrraturia for 3 or 4 daYs
uteteritis
horrhagic
Necropsy findings ircluded pyelonephritis,
rryttsrt-vrs
befoiET6ffi.
and mrcoheuorrhagic cystitis.
LaboratorY Results:
CorynebaccerlLmffit
.
-
t
Contributor's
s
Cor
@
was cultured frcm the grinary bladder and ureter'
lfucinous cystitis
is&
associated with
I'rcrs-been associated
suis tlas
corynebeggeritn uurri
itm UofyneDacEeErutr
baCteffUm
anaefObiC
dnaerobic bacter
wiclrcysri t i s ani-py;1;;6h'ili'-i"- *@l*31**?P
:f3a
rl:"g:ETi':_ll:h.
adult.'"1g".:"1'I
fi:-'*#:''i-nilil ;a;;;;fi;-"i-r,""rrr,i
g,*
;i"&;;yl-lglry
rfi""i3difi;l;;fi;
tt
{t::9^1î:l::::"'-Y}:},
glandulrlar@
*,"us-secreting,
io-"
il:pil::'
BlioffE;ndff"';;';ii: il;!i.i.iil=ip;4"il*
seemsto-be cormDnwich the infectim.
Attendees' Di
chronic, urinarY bladder,
rucinous, md hyperplastic, subacuceto
Pig.
Ilacterial
cystitis'
Etiology: goJcvnel?cterir-mStuis'
cystiEis'
ccrmenrs: oEher or.agnosesffi-tia.t"d wgrg-polypoid and hyperplastic
dif ferencially weie gl $y
Orher bacrerial
$]$^*:
"g""t.-"F"siaerea
rhe lesiqrs cencered on rhe rrEraprasElc
of
ffi;";;-;le
L 4
.Discussion
normal
resDonseot Ene urotheliwt to llucus secretirg cells rather than the
in other
reporced
mi" r""= mucSsalchange.hasbeen
fififfi.*r-"]i-"pirr,"li*.
coliform
wich exuaXtive epidermititis,,
infecciqrs in swine and has been
""ro"irt"d
cltt'Si is reported.to occur
retaplastic
;i*ilar
A
engeritis, and tol-"[of"i"-<il.
Infeccion in
in guinea pigs, $vines and-htrnanswith urinary Eract infectiqrs'prema'rt and
encourtered in
sows with Corynebacterit-ur "uie G-on"i "*"o"ly
PostparEurentariiffir.lfrftFa""it''*yoccuEiiz-t+days..Gr6ss-lesiansirr1ude
and supp;rarive
pyelonephriEis, aiiation of the ureEers with exudate in ttre luina,Discussion also
cystitis.
mrcinous
to
the
in addition
and hes'rrhagic ;t;i;i;,
in the lm:ina.propril and edena of the
infiltrate
inflarnrarory
mild
the
included
positive
bladder waII. characterisuics of-tn" organismdiscussed-wereGram
aipitr"roids, anaerobic, and restricted redia requirerents.
- 2 -
Concributor:
P. ol-E]ff58f,Ti
VeEerinary Diagnostic Laboratory, University
of Georgia,
f ton, GA 3L794.
References:
F., Cottrell, R. and Delez, A.: Ifucinous degeneracianof the
T.T6ryD.
epithelim of the urinary tract of swine. Vet. Path. 8: 485-l+89,1971.
2. Soltys, M. A. and Spratling, F. R.: Infectious cystitis and pyeloneptritis
pigs:
of
A preliminary cormunication. Vet. Rec. 69: 500-504, L957.
3. Soltys, M. A.: Corynebacteritmsuis associated with a specific cystitis
and pyelonephritis in [igs-=EE:
ffi.
8L ML-446, f96f .
CaseIII
- 7L53-79Dor 7153-798(AFIP L7L270L.
+ ,
veterlhEiian because of a hard 1.5 cur swelling
upper canine tooth.
Setter was Presented to the
of the gun at the base of the rigbt
Radiosraohic Findines :
C*pa;sne fytic lesion of the maxilla at the base of the right canine toottr.
The lyEic lesion is surrourded by a uniform thin rim of sclerotic bqre.
& Cmuents: Epidermal inclusion cyst of bone.
ConEributor's D_iggpo.sis
Corents: The lesion was curetted and 2-3 cc of keratinized debris was
renoved. the tissue resoved frm the cyst $rassubmitted for histopath
exanination. At the six rpnth follorup the lesion had not recurred.
Attendees' Diaenoses and Cauents:
zing squamousepithelial cyst, alveolar
bone, maxilla, dog. 2) lticrofilariasis,
blood.
Cqrupnts: There was considerable variation in the preconference mrphologic
diagnoses listed by the participants.
The diagnosis given above was reached only
after considerable discussion and consulcation. Attendees considered other prirnary
and differential entities such as dentigerous cyst, keratinizirg and calcifying
odontogenic tr-upr, anreloblastic variant, and gingival hyperplasia. Possible origin
of the tissue was discussed but there was no concensus of opinicr.
these irnluded
embryological rests of odonEogenicepithelirm, domrgrorEhof gingival epithelirm,
and sequelae of trar-uratic insult with irplantation of surface epitheliu.
ltle bcrp
lesions described and vieHredon Ehe submitted radiographs were discussed and felt
to be secondary to the expansion of the cyst with keraEin. Bcre resorptian can
occur wiEh any expansile lesion over bone without being involved in che lesiqr
directly.
Ttris lesion lras considered to have sm but not all the characteristics
of the hrman keratinizing and calcifying odontogenic cyst, as large quantities of
keratin are present and areas of mineral deposition are present. The tlmr lacks
the uryxcrratoustissr-p surrounding the turnr, and the conspicuous ghost cells for-rrd
in man. At the end of the discussion, it was felt chat the lesion probably was of
odontogenic origin but could not be definitively determined. t-licrofilaria were
described in the tissr.e sections by several attendees.
- 3 -
UontribuEor:
l'ledicirel-Ilffis
DeParEtrEnt of Vecerinary Pathotogy, College of Veterinary
ity of l'lissouri , Coluroia, l0 65'2LL-
References:
C'enetal As
Bone
,
3rd Edicion, Charles Thmtas
2 . Sb a f e r, W. G.: Textbook of Oral P
Philadelpkria, L974, P. 249.
f.-EElI5,-D. c.:
ts and Data on 6 221 Cases.
4th Edition,-I,1. B. Sar.rndersCo.,
Case IV - t"G 78-430 (AFIP L667745).
---i
f borh sexes appeared ill
14 days aiter arrival
uoruidiiy !{as over 507"with moderatercrtality-
and
hair,
ruffled
lassitude,
extrellE
"*r"r.Jui*aliexkribited
mice
Clinically, the affected
-
+
rrom a
dyspnea.
Laboratory Results:
1:630 titre
to Sendai virus'
Contributor's Diagnosis & Cquents:
pf"r
consolidatim.
Etiology:
Severe, subacute, digsgninated hperplasia and peribronchiolar parenchyual
Sendai virus.
. r - _ :- ^^-1-^)1., L.*^rnraar:
hyperplastic
courenr: Th;--;;l,r*tai-epirhelir-cn of the bronchioles is markedly
brorrc'hiores
the
of
lrrnina
The
wirtr-earii-tocat-ar;;t;i-rqi.,*ur-reraplasia.
detritus, and the peribrorrchiorar
of
conEain a ncderat"-io f"rg,"'*ou"i
"i[urar
with a mixed but predcminately
nas.a-vaii.b1e"d"gr""--or-"o""oriaution
parenchy,ura
nononuclear inf iltrate.
and uetaplasia
and
:ffia,
iolitis,
with bronchiolar hYPerPlasia
interstitial,
subacute, disseminated, mderate to severe,
lung, trpuse.
Etiotogic Diagnosis: Viral pneurnnia'
EiiofoEy, Sdaai virus (l4yxbvirus parainfluenza.ty* I) . .
nniptroiogic-diagnosis iisted-!y *"t cqrference
Ccmrn"ntsr nt;;;""*f"t.ttle
mdifi.rs-such as hyPerPlastic and
participant" *"r--br5""f,op"",t"iawilf,
suur"ui".--Ho*"u"., based on the iririt eEiology and.che
chroni;;-*a
ffi;pfl5aic,
thi; diagnosis was arended. Pnemnias causedby
diffuse intersriiiri'"f,".tg"",
aspiration are traditionally classified as
baccerial agents and chos6
"."onJrry-tE
and irritanC inhralatiqt are
'The
Uio""top".,-frniasl *n"r"rg viral, c-trfanyaiil,
iesicrs in chis case tdere considered
p",",.d.tirs.
classified as iniirstiEial
quice characceristic of upuie Senaai virus pner-mria. There was cansiderable
changes in the-bronchioles, cotpled
discussion of *,"-tryperplastic *a *t"plaslic.
with the mild mixed infimnatoty response, with particular refererce Eo squ@us
in serdai virus infection involves
n""pti*rffiJpt.iogic'chang!
*-"pr""ii.
necrosis followed'uy prbrieeiatioi of rhe-brornhiolar epithelirm which my Progress
occlusiim'of the h.mn. Subsequently rhis proliferative change
l;-;;;l"ce
- l + -
(:xtends inEo the peribronchiolar alveolar spaces. SqumousreEaplasia-follows the
proliferacive lesions in both areas. the cbll of origin.of che hyperplasti: .^
Ttris cell is also Ehe precursor tot
bpitheliur is believed to be the clara cell.
Type II pneurDcytesin turn replace desqumated.type.I.
tire type lI pnersocyce.
'
preurriiytes. A n,rn[rer of ittendeis repoited seeing intiacytoplasmic eosinophilic
inclusion-like bodies within the hyperilastic epithelim alcrg with a few mitotic
iirtricytoptasmic inclusion bodies have been
figures involving similar cells.
intianrrclear inclusiqr bodies have been
rrDuse
scrains;
in
convEnticral
reforted
and intracytoPlasuic.vila.l particles
inEranuclear
both
mice;
and
in
nude
reiorted
IIDuse
strains infected with Sendai
nude
and
conventional
in
have been observed
gne.atcendee
formatiqr in their sectiqt,
cell
syncychial
on
cmrented
virus.
Syncytial cell
in
cqrditiqr.
finding
this
usual
this
is
not
the
ho^rever,
formation, however, is knournEo occur in tissue culEures infected with Sendai
virus. Mice, rats, hansters and guinea pigs are susceptable hosts for Sendai virus
infeccion. lbrbidity and rnrtaliEy are highest in nice,. especially youlg animals.
Prevalence of unuse bolonies infected ranges frm 40-50%in the U. S. The
coplerent fixation test is considered t$ test of choice for detection of infected
aniirals. Tbe CF test is rcre sensitive and persists longer chan the routirely
of che lesiqls of
employedheuragglutinacion inhibition EesE. Differentiatiqr
Seirdai virus and pulmnary neoplasia is based cr Ehe distribution of the lesiqt
rather than cellular unrphology tîtrichcan be confusing.
Contributor:
Cmparative Pathology Section, Veterinary ResourcesBrarrh, MH,
Bethesafa-:m--tr014.
References:
rcI;L.H.,Kovatch,R.M.etal.:PathogenesisofSendaivirus
infection-in mice. Am. J. Vet. Res. 32: t$5-f841, 1971.
Cell-rediated iurrunity uo Sendai virus infection
2. Anderson, I'1. S. et al.:
in mice. Infect. Iumrnol. 15: 239-2M, L977.
Susceptibility of inbred and outbred mtse strains
3. Parker, J. C. ec al.:
co Sendai virus and prevalence of infection in laboraEory rodents. Infect.
Imnnol. 19: 123-130, L978.
4. Ward, J. I-{.: Naturally occurring Sendai virus disease of mice. Iab. Anin.
Sci. 24: 938-942,L974.
TIIOIHY P. O'NEILL
Captain, USAF, BSC(VC)
Registry of Veterinary PachologY
Departmeit of Veterinary Patholory
- () -
Hiscories
AEIP l.IednesdaySlide Conference - No.
5 Noveder 1980
Case I - 968-79
srtained
lesians.
1715366
'rt
beggr sali\rating
Ealian
shepherd dog reportedly
At EcroPEy, ghg rirngc erE smrtrat
cqlSested d
&rvulsicrs.
greyish foci, 2 to 3 n in disrpgga.
groes
llrere Ere rD ofu
mrltiple
case II - 82t#
L7L49L3
an adult male horse Sidr bad a bistory of
ar*ia, crd irmcdimFigrs fior 5 mrttrs, uhich inclu&d cirelirg,
e hs
tiqr.
llp cl infr-el slggs had been gecting progressively mre ftrlene, d
rms euthanatizd.
A uass was fourd in the btrain, rypering to be uidtine in origin, eritb intlrio
16es furmd to
of bodr lateral s€ngsisles. Tte maes extelrded frcm cbe orripirar
aspect of tbe cFrehlr.
[re ceebeflin
E ffi
about 4 cn short of the eiterior
inrrolved. There ms s@ necrosis in tt b,rrt.
case nI - 80-1 (eilP 1757055).
bel.o$r ttre es
Case IV - 78J.+656
of a flying
sqldmef.
1,5,6
uere t€ceived
frm
a croes-bred
r€re arreria,
sick for 48 days. Tbe nain sptms
progressive Eakness. She aborted at 89 days of pregna:cy.
A-yec-otrd
fesrer d
tntgEil P. O'I,EILL
Cqtain, IISAF, BSC(VC)
8eg:istry of Vecerirrry Pattnlog/
of Veteriotry Peofqt
Depa@t
s
Resulcs
AFIP l,,lednesday
5 November1980
Case I - 968-79 (AFIP L7L6366).
ralian shepherd dog reporEedly began s_alivating and
At necropsy, the ltrngs were soewlrat congesLedand
went-iii6-convulsions.
There were no other gross
contained uurltiple greyish foci, 2 to 3 um in diaeter.
lesions.
Canine distesper and pul^mnary
Concributor'@
toxopE-F
Cmrents: Nrserous toxopla$na organisms are present in foci chroughout Ehe
lung. In the stqnch Ehere ire many_intranuclear and intracytoplasmic eosinophilic
inclusion bodies in Ehe gastric glands.
Attendees I
&
lbrphologic
diagnoses: l) Pnetmnia,
and intracytoplas:uric, gastric glands, stoncb.
Eciologic Diagnosis; Toxoplasunsis and Canine Disceryer
Virus (I,lorbillivivir us) .
Distemper V
Canine Distemper
sp. and
and Canine
Toxoplasma
Toioplasma sp.
Etiology:
Etioloiy:
not
were
lesions
the
Toxoplasma
containing
ffitft'ns
C
q n r e n t s:ffi
i o n sco n ta i n i n g th eToxopIasnaIesionswer
enot.Pr
esenE
Conrents:
diagnosed
was
noE
pner-uonia
necrotiZing
the
therefore
submitted,
66rall the slides
the
all
was,observgd.fl
pnetmonia
The diffuse interstitial
ParticiPants
by'all.
several noted intranuclear eosinophilic irrclusion bodies in the epithelial
"id
cells of the bronchioles. All the paiticipants observed the intranuclear and
inrracytoplasmic eosinophilic incluiions iir the gastric-glandq. A-few parEicipants
nentioned-degeneration and necrosis of gastric glgnq cells and.preferred a
pneuronia and inclusions in
t{e interstitial
diagnosis of"a mild acute gastritis.
thJstqrach were aEEributed to the canine distennpervirus. Those chaEhad secEions
containing the Toxoplasmalesions cmuented on the large-nuder of organiTt" boaf,
wiChin cells and frie in alveolar spaces. The necroEic lesions corresPond to the
A brief discussion followed cornerning the
greyish foci observed grosily.
*iripf"
simultaneous infection of these-agent-s. These conditiqrs frequentfl are observed
Infecfiqr by the- virus depresses Che the_imr'rnological
io[etner in young anirnls.
of chl anTmaland renders it susteptable to infection and proliferation of
"r!tu"
coxoplasrnaorganisms. CblSsigns observed clinically in these anirnnls could be
Exanination of Ehe brain microscopically could
attributed to"either infectioi.
reveal lesions of one or boch infections.
-f----L--
:^:^-
LL^
d^--^-l
ConEributor: Veterinary Diagnostic Laboratory, School of Veterinary Medicine,
oreg6?l5ra:la-cffiver s i ty, Corvalli s, 0R 9733f .
References:
K., et al.: Pathology of Laboratory Animals. Vol. If.g"f-t"a'ta,
NewYolk , -L978,pP 95-and 370-37L'
Springer-Verlag,
'
Z: Capen,d: C. and Cole, C: b:: Puhpnary lesions il-dogs wich experirental
and natur"ffy occurring toxoplasnnsis. Path. Vet. 3: 40-63, L966.
3. $bCullo"gr,,-g. Ec al.: E-pJirental canine distemper virus induced fyryhoid
depletion. Am. J. Pathol. 74t 155, L974.
case II - 82446 (AFIP t7-l49lQ.
an adult male horse drich had a history of
ffiiffi--*.
aLaxia, and incoordinaneurotogtc slgns for 5 u'nEhs, *Iii"ii itt"fraua circling,
the horse
#a'been getting progressivEiy mre severe, and
cion. Ttre clini""i-ri*r"
was euthanatized.
in origin' with invasion
A mass was found in Chebrain, appearing !o.bt nidline
to
The mas!-exrended f;6r itre occipitafrobes forward
of both laterar ventricles.
noE
was
The cerebelluo
about 4 csr shorc of the anterior ""p""t of the cerebrr.m.
turcr'
the
in
involved. There $tas scrlE necrosis
ncs: Pineoblastoma'
Diagnosis &,C
Contributor's
.rTretunror
an$ ylas coposed of smalr
a."".iy-"ellular
tqffi6'.
conents:
"",
,*,i"t had nyplrchroaric nuclei and very sparce
round ro slighrr;-"i"g;rca-""iG
-There
The tLmE was very
was no apPaTeg!differentiation'
cytoplasmic raterial.
Twor cells had seededouc the
vascular. Ttrere was minimaf miiolic-l|tiuity.
is apparent'
ventricular walis"i" *.u"t"f- foci'- The simi-laritv to sedulloolastcma
with the diagnosis of pineoblastoma' Sm
ltre origin of the tw,3r oo",
"o.rritt"nt
tulDr for- Ehis grouP of neoplasus'
favor the Eerm primative neuoectdermal
to
i"giiir"iii f;*d'i0.5r-oi
snirnels
a series oi +oorLmrs of the n6rvoussvstemIn
l{hile no
be neuroectodexmalin origin, but none aPPe"t"a-io-Ut-pln""9tastomas'
animals, iinealma has been reported
reference has been found of pineoblastqri'in
in a horse, a rat, ffid silver fox'
Attendees'Disgnoses&Gmrents:
,
rneuroectodermal tr'[Ircr' brain' horse'
aiagnosi.-*". reached after soe discussion of the
Ccnnenrs: Td;b"il
gl-ioblast@a'
lesion. Other ei;gng;* givEn included; -ascrocytotrla, ependyurma,
Nluerous
tissue'
netvous
of
oeliodendroma, and"undiffErentiated mixed ttrpr
mixed glima
and
uedurloblas-toma
as
such
diagnoses also
differenrial
crented
morp-hglqrgl
and
""."-i""ruded
in addition Eo tfiose listed above. Cellular "o*titu.ttts
lyrphocytes'
reserrbled
which
cells
on were me preJmri"""i small lreul.tttootic 19gd with trabecular sEructures, and
larger less abtrndantgal,g ror.nd'lelk associaEed
nuclei arranged in
small hyperchrmric spindle-shapeJ-""ff" with carrog-shaped
tyPe was not preslnt in all sec;ions' Tf'"
bundles *d urtrorls. This laEt.i-".ff
was cmrented on and felt to
location of the lesion and destiuctiott 6i the neuroiitspeciar srains ingtu$ing
or *re-ii"i.a-"*plsqrnc.'
be arEriburabre ;;-;;;";;r
woufd Ue f,efpiul in demnstraEing-argrrophilic cellular
Bielschcwsky's
"i"i' *ittr pi.,""f-ifata
trnprs: Bot! [ineafmas "ld pineoblasEcnas
Drocesses
""ro.i"tfi
having o'urisntnt behavior with invasion of adjacent
fr;; b";; ;;;;;iba-as
structures.
Angeles Councy
Contributor: Ccrrparacivel"tedical & Veterinary Services,^|o"
o"p"ffin:ffi"lth'Servi"*",iigz4EricksonAve'.,Downey,CAn242.
References:
che Nervous
O. S. & Rubinsrein, L. J.: PathologYof Tr-uPrs of
ffi
L977, pp 283-298.
svsterr. 4th nd., Williams & Wilkins Co., BaltirDre, of the-nervous sysEem. In
r,ugi;Uuttr, H. t Conparative aspects -of -tr-nprs
19 63.
in'Animals' Ann' N' Y' Acad' Sci. I08: 702- 72L,
Epi"lotloi.ey-;f'c"ncer
- 2 -
Case III
- 80-r (AFIP L757055).
ated belcw the ear of a flying squirrel.
Contributor's Diagnos.lei Psorergates spp. infestatiqr.
Attendees'
Eis, proliferative and pyogranulmatous, focally
extensive, skin, flyirg squirrel.
Etiologic Diagnosis: Acariasis.
Etiology: Psorergaces spp.
Crents:
FscrTptive terms for the dernatitis turnd in before the conference
by various atEendeesincluded chronic-active, granulcmatous, suppuracive, chrcricsuppurative, and papillary-cystic.
The above diagnosis was agreed upcr after src
discussion. Differential etiologic agents listed were Sarcoptes spp., Notoedres
spp. and Cheyletrella spp. Charicceristics of the lesidfficEillo'n
rdeEfrEe
+ r , . +
mixed inflaruratory response co the presence of the mites, the close association of
the parasites to hair follicles,
md the tunnels forred by the mites in the
epider:nis. Photographs of the gross specimr frcm this animal were viewed along
with photodcrographs of the parasites. Grossly the specimr appears as a
honeycorb or rn:ltiple microcystic mass within the skin. Features of the parasites
included a ror.md body with 4 pairs of legs each with 2 terminal hooks and a
medially directed spine at the base of each leg. Histologic features include a
chitinous body wall, appendagesor sites of appendageattactment to the body and
striated skeletal rnrscle within the bdy cavity. The snall basophilic spherical
structures noted in many of the parasites were interpreted to be elmnts of che
reprodrrctive organs of the mites.
Contributor:- Dr. C,eorgeA. Parker, Veterinary Pathologist, llf0l
CourEl-6re:i6ZTElfls, VA 22466.
Stremrriew
Reference:
f-Iyrtr\
R. J.: Parasites of Laboratory Animals. University of Iora Press,
L973, pp 460 md 478.
2. Soulsby, E.J.L.: Hilsdnchs, Arthropods, and Protozoa of Drestic Ani'nals.
(l,bnnig) 6ch Ed., Willims & Wilkins Co., Baltirnre, 1968, pp 499-51.
?
case I\/-:-29-66
(Aqq-I66702r)
ed 4-year-old sow
\dere received frm
"I9:::3:
"
fever and
fot 48 days. The main sptotrEt were anorexla '
*,i"Hffi.n
"i"t
She ab6rted ac 89 days of pregnancy'
ilA;.;;it"-*"t"."tfibrinols pleuritis and
Laboratory Resqllg.i On postnorEemEhere was *i"t8!{1
very firm and yellowish
effuJion. Liver
"riioeys- and mesenceric ryqh
reriffial
"."
brcxmish.
bn secrion. splebn was enrarg"dl-i!;i"t-araspots weie scattered throughout tneit
whiUe
nodes were larg.i-tt..r-.ro"ttf"*i
parenchYma.
leukemia'
Contributor's Diagnosis & Cmupnts: Eosinophilic ryeloid
a, eosinophilic, liver,
kidneY, and
spleen, pig.
'there was general agreementwith- Ehe contributor's diagnosis'
csrpnts:
rcre eosinorphilic
lbdificarions of che satrEaiagnosiS-iG;d Ui-F:.ggt.icipants
diiferentiated eosinophilic
rnreloid ,"r"*",
ir""uf*ytic"salcma'-;rrd.*6ff
the-large eosinophilic
nveloproliferative disease. Ari-;he ;rienaees nored
oc&siqral indented and
;;lit-*a
s,lanuies wirhin the cyroplasmoi d;; ilPiFaic however,possessedromd nqrrobulated
robulasednuclei. the urajority'oF'in"-"Ll1r,
brooa vessels reinforced the
nucrei. tte presenceor lnes"-rftilri"
""itr-wittrin
Grossly rhese lesiqrs have been
*Gt.
reukesricnarure of this conairioi-ilHi;
tisiues in cattle and pigs'
described g""!i Ji""oior"a "i".s affecting various
",
resiqrs fila tr"u. to 5e differenEiated frm eosirnphilic
I"Iicroscopicalry-ir,"r"
iio"-;iis wtrenpresenr in nmrscle. Eosinophils
ryurphadenirisai-'""ri"optiii"
well lobulated cells
Dresent in the o.her conditiq," i.uia aPPearis mature
cel1s.
neoplastic
iumatureappearring
SJellJa-io-ri.".
of Agriculture'
Contributor: Animal Pathology Laboratory, QuebecDeparrenC
7C6'
25
St. fy?ffi-t6'eTrrebec, CanadaJ
Refgrgnces:
r-,Jr ^-r r--^r^-^rê'
and heq?topoiecic
J. E. and Dungworth, D. E:, - Itmrs of lphoid
ffi
Editor,
cisstres. rn rumrs-in Dcrestfc gtrir"t", 2nd Editicr, J. E. lbulEon,
c"fifornia Press, 1978, pp 150-204'
u"i".i"ity-t
Natimal Carner
2. Migaki, C., Heuratopo_i:li"-ry*pii"*s of Staughter Animals'
Institute"l'lonograph 32: f2f-89, L969'
P. O,NEILL
TIT,tOftIY
Captain, USAF,BSC(VC)
Reiistry of VeterinarY PathologY
OertrmLnc of Veterinary Pathology
- 4 -
Histories
-No.8
AFLPl.tednesdaySlide Conference
U Nove6er l9g0
Case I - SI'IRIRat Case II (MP l7f8l/f)'
approximately lO.t*"f
-lstor-:Tji
,,i*,ffi(.'a:diare!er)massinttreri!trtventralsideofneck.
Case II
- D78-9390 (AFIP 1668449)'
with a hisgory of ar acute b.,s"t-oi
old rat presented
female cross-bred ffi, afuitced to ttre clinic
anorexia, depression srd head pressing'. The
Dearhoccuired wichin 24 trotrrsof atuission'
;;il1
6p";;;d-;G;lt-aer,yaracea.
sptrc'
Threq"rnert r.po.l"d *rat anolner EBre died recently wirh CD{S
Case III
- 25903-N
P L157436
a doe besan one mntb after it had been hic by
E6ifle@E-in
ard alveolar pner-mria.
revealeddiffuse inceistilial
Case IV - !lA8-If9 (AFIP f6687t2.
Historv: Arl
Payed f9t"f9 cat with a history-of. posterior
Euthanasia was
and trearrFnt.
ataxia rot I week$ ilas presented fbr'exaination
pei-orrea 2 days after iosterior paralysis developed'
TII-,1I}TTP. O'NEII.L
Capcain, USAI', BSI(VC)
Regiscry of VeterinarY Patholory
Departuenr of Veter inary Pathology
Results
Conference - No. d
Slide
f^lednesday
AFIP
12 Novenber 1980
tlase I - SlîRIRat Case II
with--Ie6
(AFIP l7f832l).
approxirnauely l0 -wee[ old rac PresenEed
(6 cm. dianecer) mass in che righc ventral side of neck'
Contributor's DiaPFosis & CqmEnts: Carotid body tull)r (cnerpdectorna;
nonchrtrBt tln ParagangrlCIna)'
Ccmnent: tne E-urflr consists of round to oval epicnelioid cells wich
Cells are arranged in cords, nests, qd
eosiiophiii"-;tiopf""*.
indistincr,
tiUriu" trabeculae divide Ehe Er-lrpr inco an alveolar
In *,"y'"r"u"
cl'sEers.
The'cumpr is highly vascular, and a-Peritneliomatous paLEern is
"ii."g"n="c. MitoCic figures are rare. Many areas of necrosis are presenc'
evideXc.
Attendees' Diagnoses & Cmtrents:
o{na' malignant, vencral neck regio.n' !a!'
l
Prirnacive
before the conference included:
surrnitted
Ccnnengs: othei diapnoses
turpr of primaEive cell origin, poorly
neuroecgdermal .lr"pf"",rl'*Jitiur"ntiated
and reciculLrn cell sarcqra. CurenEs on Ene rorphglo,6y
dif ferentiated
co spindte""r"'in*,
of che tumor incLrJ"a ifre prcxninent endocrine paEtern, Lhe small round
wirh scanc cyloplam, cn" variabl! mirotic raLe, and nulrerous foci of
ilrpd
race
""it-r
necrosis. The cLrrDrwas ioniiderea to be malignanc, basedon,Ehe hign uricocic
into
extension
wicn
Ehe-caPsule
of
invasion
observed in sme areas of the tr-srrr and
is
ionsidere!
tuncr
gylc:^::t:,in
This
tissues pi"t"nr in sorresections.
These tuII'Drsare trDre often
"aj""""t
Ehe raL, as only bne has been reporred previously,(l).
of aorcic body origin'
are
oi'them
tn"joricy
in che canine, where'itr"
encoungerecl
than are caroEid My
frequent
nDre
ciues
Aortic body tulrnis in che dog are 5 co 6
In mancarocid body
similar'
t-tphologically.
tuncrs. nistol<[i.":-fy CnuEto utu
The functions of
tulDrs.
forfic'hrdy
tna.,
frJrrDrsare encounferedmore frequencfy
chesorecePlo-I
These
reviewed.
were
bodt
body and aorcic
in.
anclalO 1n
""rorid Eo changesin blood carbon dioxide, ptl, and oxygenCension'":91":.1t:sensiCive
an
Cheregulacion of'respiraEion and ciruculation., Theseorgans.can initiaEe
paraminute volr.meof respiraEion by way of
increase in rhe-;#[:-;;a;-;"4
nearE race and blood pressure Dy way oI
increase
and
synpaEhetic innerviCion
sympathecicinnervation. These i*ot" in dog-sare cqlsideied co be nonfunccional'
is Etlou8hE
Tlre exacEcefl ol orig,in has not been precisSly determinedalthough-ic
neu-'-oof
cells
by
Eo arise tr<xr perivasEular nnsoderrnalbelk chit are invaded
jugular
utlt
internal
This tissue also is found in the
ecroderffiaforigin-tij.
of,
branch
recurienc
Ehe
along the innmri""i"'arreiy, U"i*r-crre middle ear, along
ganglion, Pancreas,ind che ciliary ganglicn in
rhe slossupnurynt*;t ;;;;;,'nodo".
tire orbic (2) .
lzt++ '
i,)onCr
ibuEor: SCerling-Winrhrop ResearchInsciCuCe,Rensselaer' NY
References:
T. SII'e,
p. n. et al. :
fun:rs.
In Pachology of La'roracory Ani-sBIs, VoL' I[ '
s;;i;;;;-v.ir
Benirschlie, K. ec al - '(Eds)
f u u n o' r so i cnE endocr ineglands. In
.. , ,.\
W
2 . C ; l 1 r e nC
, . ri.:
(gd) , Univ. oL r laLifor nia Pr ess, r ler lceley'19{ 9, PP,+r +- +z u'
]'lculron,
I
[.
.
iinals,
An
----J. gertirscn('J,
Pacnologyof Labor acor yAnimals,VoI' II' L) /6'
K. ec ll.:
p. t0fttt.
' [r - cfi]r s,
VOI' i'
of
4 . A s ,rL e y,l )..J.8 .: E va n s'i' listoL.l,licaIAppear iinCes
i r 1 i i d i t i o n , L ( l/ ' j , p P 9 / - ' i I .
Case Ii
- D 7 8 - 9 3 9 0( A F I P 1 5 b 8 4 4 9 ) '
- ^ r n n ^ ^ . { - . i r r a r r n r - h ecl.ini,:
etini:
fer*rle cross.Dred PoA a&ricced Eo tne
, o i . t " ' f f i r o " y . o f a n a c u t e o n s e t ; i " " * " * i " , d e p r e s s i owiEhin
n a n d . h24
e anours
d q : " :ot: i : adfiilsslon'
9:.Î?i
aLrinal appeared--""ruiy dehydrated. DeaEhoccuiredwith CNSsyilPtolls'
recently
fhe oivner" ,"porru6-that anocher mare died
Leucoencephalcnalacia(rploy eorn
,l)nErib
poiso
diasEt
î -^l oaia
aaoh ?
- l ccn'
n . iin
n d
iagEcer
2-3
each
ccmrenEs: on gross exanination ti^to areas oi malacia,
organs'
other
in
fowrd
not
were
were found in crre Eerebrar white-matter. Lesiqrs
fot "nn.I{I c-rtt poisoning" in horses nas noE beenEo horses
'F;;;,i.uq
The Eoxin ,""iror,"iUiu
Tsohred from feed Eoxic
*.*'toi"*"n!"-u""n
characEerized.
ptoou.iio" of cne Eoxin wnicn causes cne
and is choughc.affipffiffi
d isease.
AEtendees' Diagnoses& CosEnEs:
acute, nurci focal co focally
lor6;raamffiphalcnalacia,
Eortical white maEcer'brain' horse'.
extensive,
".u"r",
EcrologicDiagnosis:l,eucoencepnaliticmycotoxicosis'
Et rology: t'l"Tdt;g1n poisoningl rE?,iiHt.F"iliforse'
i."uprabie mor3i55ogi6ni5g;5516' lisced by runy
Ccxnrencs, A"-u(*ify
whiEemaEEer'
parErcipan6swas neirosi's, md freunrrnage,acute, uultifocal',severe'
b r a i n , C r r a r a c t l r G ; i ; ; o f * , . l e s i o n s c r e n t e o o n w e r e t h e - l ainflamstory
r g e a r e a s resPonse'
necrosis pru""rri- in the tissue, ihe lact< of a sienificanc Considerable discussion
vEssels'
and muttifocal henorrhages, mst ;oto,," .i"*a
trDsEnotaoly cne hyPerEroPny
vessels
around
and
in
ensued concernirif;;;fi;"g"s
cells in small vessels, Ehe perivascular
.md possible hy$rplasia oI
of small
sue eosinofnirs,"na EhePresence
*o fiJil"il".i"fi;;i "nao*,"iial
heanrrhage
of *rE vessels' Tne-Presenceof,:1:t"
variaOly sized hyaline bodies "t'"*a-t*"
tead poisoring in nutkeys
hyaline bodies in o*rer coxic CNi conditiqrs- such as to Loxic enEitres' Tnese
a iefationship
i-coirefatioti'*iin-oi
srrong,ly
been observei in mrkeys poisored wicn
have
"ugg""l"
pAS
positive. They also
bodies are
"unldy corn poisoning'-' in horses are may
arsenic. ocnertilsi;;'"rl*'i"c"a-*iir,
uire'duct Protiferation ifiich
centrilobular hepaEic necrosis and fibrosis ana
cNs lesions alsc may
i; coniuctiqr with it.
occur indepenoerE-.i-.ii"-aNs-lJi;"-;; pifi"t"*"t
lesions in rhe
(rt."
ai"gnoti"
-Lesions
9f ,ylacic
occur wichout hepatic lesions
in cnis nerpeEic
Edii" n"ip"" Virus I.
equine brain
mat6er' hesDrrnag'e
white
Che
to
"rriSliîr.til;
rnalacia which is noC ristricCed
disease include
cuffs' 0rner
fyqh&ytic.perivascular
-silr:,Td
arounrlvessels, a necrotizing itt"iitit-""a
llsions include i{EE' vEE'
diseases of the equine which m"y-proarce clls
and PurPurahmrrnagrca'
nEe, -quine viral'arEeritis,
Ccnributor:
i l-t iffiTs---'I8CIf.
Veterinary Diagnostic Laboratory, University of Illinois'
Urbana'
P.eferences:
an
-At. thenEjor-cause of
f.. et al.: l,{cldy eorn poisoning.as
T.ffiIilaH
J' Vet' R'es' 29: 202-2f,9' i968'
encephalanalacia syndrmre in nifrcir"
"Wi9"9.
A orycoCoxicosisof equiCae
2. l,tarasas,W.F.0. eE aI.,-'ftu.o.rr"l"phaloralacia: J' Vec' Res' +3 (3):
cirusedby trusaririmrpniliforuB #;id;;l--ile;[!p-tt
rii:izz.' LsE;-
CausaEive ly1gus agenE of lerroencephalmalacia
3. liilson, B' J. eE aI.:
Vet. Rec' 88: 484-48b' L97L'
equine a"itnufi.
- 7 -
i:t
(q4! u5713b)'ca-rerlr - 2o903-N
ic irad been niE
in a Jcg Deg'an one lpnEn afcer
biffu"u i.ceistilial and alveoiar Pne.n,onia'
."ffiogroif;;;;;;rr"i"
due to Filaroides hirUli'
;'rY
nenaUodiasis
ConCribuCor's Diag,nosis: Pul:rxcnary
Arcendees' Diagnosis- & CmsF-nts:
can ine.
ooodle,
rHtiologic Diagnosis:
EEiolrryy:
Filaroi9es
' granuluntous'
seve-L-e'lunl'
diffuse'
Verminous PneLrnonla'
sp'
,
.L>^r
tr-.' rL^
ârrininnnfe,
ffi
t ] c l r r e n c s : o E i r e r d i a g n t r s e s s u b m i e u e d b y t h e p a ihtersci'ciaf
r c i c i P a l : : - 9 ' verminous
-i*toEhe
granutuna'ous pner'mbnia'
confer,:nce rrere il;t;;i?lal
parasices listed were
inr"rsfiri"r-p*u.û.*lu.'oiii"."ntial
pneurronia,and
"h;;ia
osleri'
F'
and
Egar.llr',i* milksi
FurEnertrpqe'the tlorm was
Ttre lxrasite was idenCified as a nematode'
rhac
f*ily-r"bcascrongylidae. characteristics
classif ied as a',r*,b";-;i-ir,"
developeC)
(pooriy
size of-wJilsr-z-) ro*,
enabled this diagnosis were: I) *!,rr,
large'intesCine cqosed
m" p'"'"n""'of'a
musculature, 3) larvae in utero; and 4)
carlarned rarg,e arpuirs of heupsiderin'wicn
of few ceus. The inEesEinar cerls-also
cases oi infection
ft*'tinom
,iii";F;"i;il?bil
rhe worn
'"r,J
The norphologr of
"",g*iiaiignosis,was {i}glPi*":i,^, Specific
tr,u, ,t'q.
Filaroides spp.
a granurmacous
secEToffiffiince
was nor possiut"'il'iiil?"8i;;1
TffLiTrcarion
specific
probable
spsc
the
reactior,r was surrounding bom aduits rrnd lirvae
diagnosis was I. hirthi.
around larvae and degeneraElnB
A similar granulomatousreacEion may be observed
adulc F' milksi
worrls in F. urilksi infectiqr Uui-it--"oc'cqmnf'y-observed-aror'rtd
as.a pos'ibilFT'r-o.i4es osleri *oura also have d 6"-"""ria"i"a
'j[;;;:.
nernacodesr
wicn nodule
associated
usually
are
cE'ffi6uffir""neuratodes
Posr'tive
bronchi.
ot"tl-crr"t""-"na-i"-tn"_hrg.r
forrna.isr at rne bifurcati*
parasiEe
of Uheentire
identificaCion of the wormwoula-iequire-examination
cavity,-and reiroductive
Ut""al
lengch, characcei.tli""-ot--i6
incLuding Oooy
buEa
'Ihe i;i:;i.l;;;-F.
hirEhi has not been corpleEely workedouE
fearures.
exiscs
evidence
trosr . considbrablenollusc i,
of the -LI stage of the parasite
ingestion
"u"piiila-'"!-ir,!-i#""ffire
indicacing infeccion also may ;|.u! -lrt if,e fung, of canin6
include Capillaria
(Z). Other nematodeparasites e"*a-i"
also nuscbe arert
aerophilia, cr"IJl*,"'i"ipi",
""t 4gi:gry+t+
{ne
YasoLPi
@abscrusrnalalungr,vormot6uffimaybeanunnatural-lcsE.
of veEerinarv
Departnenrof PaEhotggy& Parasicology, scnool
*"trt*-t:
universicY, & 368i9'
unaiEfii6G;'
References:
T. aGilEil.
R. eE ar.:
Firaroides hirchi in a,dog. Facar nyperinfecrlr'r
ififfiTfl5: 33'fTJ+: l?901 . -^^
-i.-n.-er
of aucoinfection'
suggesEive
of cne life hrscoryoi
aI., P;;1iniryIy i1veg1lgation
2. Georgi,
, -L976.
nirrr,i. cornell ver. b6: 309-323
L;.ilaroides
----T.-ffirTrEE-n,
osreri infesLarion in do;-s'
strii"I-o.-E1
J. E.:
"s
j.
0ndersrep,<ro,,cvec. [""' 35: 225-256:86{
- 3 -
CaseIV - NA,J-II90ntf 1608789).
payed tamale cat wi,trr a histmY of pos,ter:ior
.rrea4pntd,ucna.lasii '*as
,rraxTa-Iffi
weeks was presented for exmi.natiqr d
perf,rrmed 2 days afcer poster:ior paralysis dettetord.
j,:rnor:t-ory Results:
t)Sf-I62qg% protein
39 wBC/mr (gJt rrehocyres)
Con,:ributor'@
nenin@EfEf
Blastcnrycosis,
granrl onqagqus
ranirges fru Cl ttlcuryn Ere carrda equina
i,(trreots:
The spinal csrd d
oermtiti.Cis
urtained
a sul-rprrirBeal p5regranulomtrvJg prces.s rittr BI"as
seeo in the lesims of eaAr sectir:n.
Tnere lse frcatr-
pyogrirnuloertcr$ Iesims
l-ett bipocmps
in the area of e
m
occr.pical regicts.
Atteadees' Diacrmses E Cmts:
diffirsg" ss!'c;e,
litis,
iltograrl,@tqJs,
spinal cord" 6H, felire.
Eti.oL€ic Diryrnsis:
$rcotis ffiiiqBmFlitisBLastovcee derilnfiddis.
Etiolqr:
diagruis arnd
raitb uE canaihruars
Cc-"ents:
rt=
i€s
icse,tE
mt
felt
signifc**.ty
c@rtsA fs participmts
ryinat omd
ringitis
aleALt
and peferred tb diagmis
sf
inuolved i.n their sectim
participants agreed that dE prinrry les'o-t nas in trp l+ninBee ritb secey
rc chf,rderizrd
extersian ioto tbe spioal cmd- Ihis extcio
V inflrmrm
d destnsio
of cre
p@f,rating into dre srsst*"re of fu cad at fu peripky
Feaares
rere deented by all.
intol.rd neuropil.
neccElg orgaisc
rcre size (5-1511), [Eeserce of a tbriu
of btastoyces agaic
charrcteristic
ttee feacwes
cell uall, d broad bfls6 h*|iry.
Speciat SaiG *ricb Mratd
ircludett tl6, PAS, ad Gridtey's hrrgus srlin.
Tbe rarte of iofectim in tnls cds*:
rrcIuded ure
*as cmjecrr.Eed to be eis ssriqgeal riessels c C$- Discrreio
geogr,aphic distributisr
of the mganim ad ttre oatr.cal soil Life cy.le. P.rlmrar.'
seeo in s1innfu, h.t stin, bc d vrsceral lesrgs .els"t
lesisrs ,rce mst cmrl.y
histrylnsrrtis,
occur. Differential
diapses irclude parrcid'ioidm57wie,
cryptococ@sis, and cmcidioi@7coeis.
Deparesrt of Veterirnry
Csrtritntor:
arheGlGTI2.
PafuLogr,
Linieer iEy of G@gid,
Refererrces:
rl[rteFi'c.
61;ma1s, Vol.
et al.:
pp-Tt60-t-562.
2- F.rmrs, C. l{. et al.:
]rd Edititn,
16, in Patfiologr
{bet.
Fugal disesesR. et al-- (Ed-), Sfo;-U*-V€r@
tahatoiy
II , Benirsct*e,
I € 6 & Fdi.ger,
Gr4ter 2L, ia Itbdrcal S-,ol,r$l,
BlastmlrcosisPbil€delphia, L977, pp.'lA2-3b1.
rrilIIIf P. o'rg[{^L
Captain, [EAF, 3SC$ru]
Regiscry
harnqt
- 4 -
of Ver-erinary Pactrrtqgr
Patnr,rtqgr
of Veterinay
Iliscories
AFIP lHnesday Slide Corrference - No. 9
19 Novesber 1980
Case I - 2 & 3 or Skin & LLne (AFIP Le0295).
e cat was presented qr 8lL5l77 to che
acce@cerinarian,hewasdepressed,hadarecta1cery.of104oFarrdarcL'r'd
purrture in the lefc thigh. Ttre anirnal was tr(,1red wich Penicillin lt'l srd
hospicalized. Ttre next day, the patient's teq'erature dropped t9 I02oF. X-ra-ys
Ct che
of the left rear leg revealed rp broken bares or ary additianal lesios.
che anirrnl's tery. dropped to 98oF. and had pale
2nd day of hospitalizacion
rnr.lcusmeqbranes. Laboracory tesEs revealed a PIV of t0% and a tlBC of 12,000. I\p
neeks folloring the first day of hospitalization Ehe patient was still very
depressed and icteric.
The rectal tenp. at the tirre was 96oF. laboracory tescs
and
revealed a PCVL07", a i.tBCof 11,000 and a blood f ilm wim polydrrosia
nurerous nucleated RBC. I\eo slides, qre each skin & Iung.
Case II
- 0ffi-962 (AFIP 1758398).
n u Z-year-old
cqrtrol
female rat.
yellovl nodule 1.5 x L.2 s
-Gross: Right kidney has a large firm
excending-Tnto uEdulla .
in cortex,
case rrr - 794386 (ArrP L7L2926).
oy poodle, 9 weeksold, was presented to a
Trearent
veterinary cqPrgerpyl service at 3:00 a.m. after a secqrd conwlsiqr.
the pup $eakened
consisced of sedatiqr at first and later by anesthetization.
A
A
progressively and died at 4:20 p.m. It was presentd for necropsy after 3 days of
refrigeration.
Gross necropsy observations were rrurcollapsing, congesEd,
edenaEouslung,s wich foam in trachea.
(AFIP U6f,l88).
CaseIV - L773L/8O-976
erved 3 l'br 80 in chis rabbic.
It died
eleven Oays laEer.
TII'IJITIYP. O,NEILL
Registry of Veterinary Pathology
Deparmrc of Veterinary Pacbologl
AFrpwednesday
rliilt8:"ference - No. e
19 l,trovember1980
2&3orSkin&
Puncture in che left
hospitalized.
P L640295
chigtr. Tfre ininal nas ir."i.a-*icnE"ilinin
-ar
The next ji{
ilay.
Ehe
E!: oatientts
pacienr,s ror,r-rrrlrrra
ternpera
id;
-io -
u.t and
rtrnnmt
l/l')oorl'-x_
;tpA rn Ioit
*"f
*" }:?id:
^Îhiî"f
. t, no broken-;.ry.
of rhe
left rear
leg reveaied
uones6r-*i-"aoiii[i}-i"r;*":
-;roei"g_
rry,
t';'r*
;il'i?;' p"t
T1,l.-lp:tr *ll:::i:i- .1.
1"i*i;
;;-'ie86:
"
pcv-5i-ro%
rescs
reveareal
.'ilid-oi-irffi0.
3:,y: P:-._.!'p?I?:oTv
ry
:::l:^::lT:l"g-*e.rirsc
qepressed
depressed and
and icteric.
v!
erw\t
r..o
div of hospirari".ii"" cheparien!vrassrirr very
The rectal
temn nr
r a,.n*^r^--- cesEs
at- rha
rhq rim
qire irac
wasoAon
96oF. rroo..cory
F:.r??.ll^.*q.
ll:9m
.-urooJ
iil- i.iirn-p"ii,.rffi;il;d
:=l*
:..ry^191,-3^!ilBcjf
nrqErous
nucleaced
RBC. fiio slides, "",f
one each skin & i*g.E--'--'
Laboratory ResulEs:
@
pcv
3rd-day:
temp. 96oF.
U,000, reccal reslD.9goF.
l0%,t{Bcrr;000, porychr*Ii", nrcrearedRBC,s,reccar
Concributor's D
is & CoutrEncs
I'f,fPfioro{5rc uragnosls:
Acuce and chronic fasciicis
- PuIIDnary thrmbosis.
wicn
vasculitis
and throtbosis formacion.'
Eciologic Diagnosis: Bacterial chromboembolicsepticemia.
Cmencs:
IE is felt thaE the thigh toould was Ehe site of che initial
infeccion wfrich led to a sepcicemia resulting in a regenerative nemlytic
anemra
and pul-unnary chrorbosis.
Accendees'Diagnoses & Comencs:
itis, subacute, focally extensive co diffuse,
-^r^
trDderaceco severe, subcucis, skin, gi:npsg qsg, feiine.
2i ttirmUosis, venuGr
and arceriolar, m.rltifocal to disseminaced, sJ#uticular, dermat,
vessels, nrcderate to severe, skin and fung. 3)'a)'vasiuii.ii,
Hlperplasia, redial,""4-il1,[;;t
and incimal,
rcderate co severe, pulunnary arceries, rI"g.
subacuce,s&llcifocal,
mild, ^venules, subculis, skiir. il pneumniE, intersciciai ana-granulmacous,
rulcifocal rnderace, lune.
Etiologic Diagnosis: Unresolved.
CcrtrEncs: The presence of the-pner-uo.niaand chroobosis in the lung varied frm
one section to Ehe nexE. the eciol6gic diagnosis was deferred, as a gEneral
corrcensuscould not be reached. Differentiil
-il;;ri;i-;;F;;;;e;l;line
etiologic diagnoses irntudeA
disseurinated intravascular coagutari;,
inrecciors
anemia, and mrreloproliferacive-disease. A proposedpossiure'eiiorogic diagnosis
for the rediai hyierplasia lisceJ-bi*"i;5"-[ur-*ry
nemarodiasis caused by
ielt ciresrin iesisr andrhe pur*r"iy
+. h,]y.parcicipants
f*:H*tg"ryl":
cnrqrhosls and associaced
lesims were
related. Additionally, a largl nrmber'oi
parEiciPancs wonderedntrether a concurrent infeccion wich Hefrobartonellosis
or
erychemic myelosis eraspresent in cnis case. Scmeattendees reporteo ous";uing
suspicious granular densicies on RBC'sand could noc rule out Hem)barconelia
organisms or Howell-Jolly bodies. Binucleared red blood cells and ntnerous
ismature cells were reporced by a few atcendees and felt to be representaEive of a
nyeloproliferative disease. lGdial hyperplasia was preferred over Ehe usual
diagnosis of redial hypercrophy becauseof increased nr.der of surcothmuscle cells
aPparenEin chese arEeries. The incimal hyperplasia was discussed and proposedro
be due to direcr baccerial endotoxic effeccs or to hypoxic changedr.reto che
chrdosis.
At the close of the discussion of this case, ic was suggescedchac
addicional laboratory tests and other organ cissue sections such as liver, kidney
and spleen may have been helpful in determining Ehe spbcific cause(s) of che
Lesions presenc.
ConEributor:
UniversiCy of Texas SoucnwesEernMedical School, Dallas, TX 75235.
Case II - 080-962 (AFIP t758398).
n a 2-year-old
control
fp.nale rac.
Righc kidney has a large firur yellow nodule L.5 x 1.2 cm in corcex,
-Gross:
.
exterrding into redulla.
Concribucor's
Diasnosis & Connents:
Renal cubular carcinma.
icy of che cellular rorphology wicn areas
Ehat are forming tubular areas, papillary areas, sheets of cells, anii clear cells
or "hypernephroa" have been previously reportd
in the liueracure.
No recasaric
lesions liere seen.
Accendees'
mrlclfocall
l) Renal carcinmra. 2) Nephrosis,
Corencs:
There was general agreeuenc rrith Ehe concributor's diagnosis and
corrrrnnEs. iitrile various narres are acceptable for this neoplasm including renal
renal tubular carcinma and adenocarcinma, che
carcinoma, renal cell carcirun,
first two nees are used rnsE widely.
The nicotic rate was low buc srrrp of che
figures hrere very prmrinent.
Vascular invasiql was noc observed. Differencially
s6re participancs felr chis neoplasm hras an adenma. Distirrccion beurreen renal
carcinomas and adenmas ofEen is difficulc.
In the abserce of mcastasis or
discinct invasion, Lhere are no exacc gross, hiscologic or ulErascruccural features
Ehac consiscencly differenciace renal carcincmas and adenmas. Even Einy turDrs
less than 2mr may exhibic feaEures of malignarny including necrosis, hmrrhage,
pleourorphism- These turDrs do rpc mecastasize
prominenc mitoses and cellular
in huunns and dogs. Ic was pointed ouc chac several chemical
cmnnly.excepc
corrpounds have been shown co produce Lesicrs in che kidney ranging frcm cubular
hyperplasia co carcinqn.
Lesions in rhe urecers and urinary bladder may acconpany
Ehese rubular changes. Incidence of primary carcinoss of rhe kidney in racs is
in rhe
considered quice low. Spcrtaneous mixed sarcomas have been reportd
A short discussion follosred ccrcerning
Osborne-tubndel and Bronn Norway rat (1,3).
che other lesisrs present in chis case. The Eerm nephrosis was preferred based on
changes ard rhe preserrce of proceinaceous
tubular, and interscicial
che glcxerular,
- ? -
casEs in sme cubules. A nluber of equally accepcable synonyms for cnis condicion
also were trEnEiond.
This lesion is presenc in many scrains of racs. The
incidence, &d age of occurrence vary wich cne strain of rac. Nepnrosis ofcen
incerferes wicn the incerpreEacion of experirental procedures.
Contributor:.
Turnpillffi,
Pamolory D,eparCnpnE,Hazleton LaboraEories, Inc.,
VA.
9200 Leesburg
References:
fTurusov,
V. S.: Pachologyof Tr.uors in LaboracoryAnirnals. Vot, I, ParE2,
pp 73-75.
2. BulleEin of the tÎorldHealcn Organization. Vol. 53: No. 2-3, p. 238.
3. Benirschk€, K. F., et al.: Pattrclogyof Laborarory Animals. Vol. I,
pp L42-L45, and Vol. II, p. 1161, Springer-Verlag, Nan York, 1978.
Case III - 79-0386
consisced of sedation aE first and lacer by anescheEization. The pup weaicened
prqgressively and died at 4:20 p.n. IE was presenced for necropsy afcer 3 days of
refrigeraciqr.
Gross necropgy observations rrere noncollapsing,-congested,
edesncous lungs with foam in trachea.
Contriburor'e Diqglroq1s& Cqmnts:
Calcificacion, rrEtastatic, lung.
on of rhe hearr or ics bfdd veisels, buc
sm fiber degeneration was indicaced by HBF" scain reacEion. Kidney concained
lesions mrphologically similar but less exEensive chan those in the L-rrg. Kidney
and lung deposits were Alizarin Red and PA,Sposicive, iron and fac negacivd. EM
photmicrogrgphs demrscraced laminated struccures associaced wich degenerating
cell organellee, with collagen fibers or with incerstitial
space. Incensely
electron dense needle-shaped crystals noted in and at Lhe edge of laninaced
struccures and in rhe lining of vessels resedled hydroxyapacice. There were no
significanc lesiqrs in ocher tissr.rcs. There wag Eensicr becrdeeno&merand oreeder
wLrichmade it unjudicious to irquire abouE che feeding routine co wt'rich Ehis yowrg
dog had been exposed.
l) Calcificatiqr/mineralizacion,
Attendees' Diagnoses& Corents:
recascaEic,
dif
e. 2) Pner-mnia, incerscitial,
granulocracous,ctrronic, focal, Iung.
Etiologic Diagnosis: Unresolved but suspect hypervitaminosis D or uremia.
Correncs: There was unanimus agreeuEncwitn the concribucor's diagnosis. Tne
diffuse mineralization in this case was considered cmpacible wich vicanin D
coxicosis or uremia. Possible causes lisced prior co the conference $ere secondary
hyperparachyroidism, ccrgenical renal hypoplasia, and fr-rrctional picuicary
adenon. A focus of pneumnia wich incerstitial
fibrosis, macrophagesand gianc
cells was present in sqrre sections. If chis focus of inflasmation was a response
to che mineral, !{e are unable to explain the focal nacure of the response vrnenche
mineral was diffuse in septal walls, vessel walls, 6od bronchiolar walls. t"lineral
also was presenc in many alveolar spaces. There was sc@ discussion of che
- 3 -
vaccinarion staEus of chis animal and che possibilicy of cne CNSsigns being
atcributed to che srecasEaciccalcificacion or scrE infectious Process, i e. canine
disterper, or coxoPlasrosis.
D'epartuEntof Vecerinary Science, Universify of Arizona,
Concributor:
tucs66Z-85721.
References:
ffi.D.,eta1.:HypervicanirrosisinNerrl{or1dl'!mrkeys.AD.J.CLin.
----2. 222 358-366, L969.
Nutr.
Capen,C. C. et al.: The patholory of hypervitainosis D in caccleVet. 3: 350-378, f968.
Pach.
--R. l{. et al.: MeEastatic calcificacign,PldY9gq in dogs oy
3. ltttig"t,
trypeivicaninoEi"'D and haliphagia. Am. J. Path. 262 45L-473, 1948'
Case IV - L773L180-976
: A severe
P t76r488
3 Mar 80 in chis rabbit.
Ic died
l) HepatiEis, necrotizing' -qcuqe,
2> Coccidiosi.s,-repatic,
iitiformis.
uufr
*,tcifo""1,seveie,tiv.r,etioro[v:@HyperpIasia,biliary,
mrltifocal, severe, bile ducE, Iiver.
,
ttre'incrah.pacic bile duct epithelirm is proliferative forming
C@nEs:
fronds with nr-rerous cocciiial organisms in- varying st€es of developnc in ctp
i"l cells and Ltren of the-ducts. AIso focal areas of necrosis are
"pi-f,"f
rEaii"r"A througlrout Ehe hepacic parenchryq. Degenerating.hepatocyces ac.che
bacteria consiscent with
of necrosis c6ntain litmrtous
marsin of che
a cruly coincidental,
"i""s
represents
piobably
case
This
il;?ii;-piiit6*ir.
Cqrtributor's
Diagnosis & CmenEs:
BoEhEireria
simrrtaneou" o"frrr".*e of two iifectious piocesses in one tisstr.
ESilaEiqr
requiring
neither
gacili"rpactrogens,
piriiormis-aie-p;i*"i
sreidae ana
-ot
a'
:ffiH*"fHll"1#5ffi"!li.*
a preolsPosll
cause of diarrht
in other tissues of this rabbit due to extrellE posmrCeut autolysis- -Horeyer.,
other rabbics received at the sre tire frm thl szne suPPlier-and colony had
lesions caused by l. piliformis in the caecLmand hearE as well as Ehe liver.
E
Attendees' DiaPnoses& CcmEnts:
lasia, biliary, mrlcifocal, urcderaEeco
ii
iiviri, iabbic. 2) Necrosis, nepacocellular,
""u.r!l';i6%*lai"ectasia,
mderate, Iiver.
rultifocal,
il Hepacic coccidiosis. 2) Bacitlary hepacicis (Tyzzer's
Eriologic Di;gt#";i
disease).
EtiologY:
f) Eireria
stedae.
2) Bacillus piliformis.
- 4 -
Conents: An equally accepEableunrphologic diagnosis of proliferacive
cholargitis was lisced by several aEtendeesas chere erasa mild inflannaEory
componenE
surrotrnding the affecced bile ducts in manysecEions. This inilarrmacory
cotrponent_was
cmPosed primarily of lyuphocytes and a few heteropnils. Large
nr.mbersof coccidian parasites were observed in cbe hyperplastic-epichelirsr-and
free wirhin the h-uen of uhe bile duccs by all Ehe participancs. Scmepreferred an
eEimrpkologic diagnosis of protozoal cholangiEis. AII of the parcicipancs
reported observing the snrltiple foci of hepacic necrosis and several reporcd
seeing intracellular
organisre in the hepatocytes at the peripfrery of chese
lesions. These baccerial organisms were characterized as long bacilti arranged in
a cross sEick fashion in the cytoplasn of hepatocytes. Subsequently a Gtr'IS
siain
was provided which accentuated this rcrpholog.
A brief discussion followed
concerning the life cycle of the coccidian parasite. Poincs discussed reerercde of
transmission, migration to the liver, bile ducts, and scages of replicatiqr.
Tne
siutulcaneous @currence of these diseases in chis rabbic suggesEsa decrease imu.ne
sEatus due co stress and hepatic coccidiosis wtrich in turn led to che esEablisrurenc
of q. pi.fiformiq as €n opportmistic infection.
Concributor: Division of Pachology, Walter ReedArmy Institute
Washirrg-t6,-F200U.
of Researcn,
References:
f.-TEeffi,
H. M. et al.:
The Digestive Systen. In Parholo
Animals,
sls. ed. bv
by K. Benerscke.
Benerscke,et al..
al., NewYork. Springer-Ver@
Sprineer-VaTlff
'
2r
2. Squire, R. A. ec al.:
Twprs in Pathology of Laboratory Aninals, ed. by K.
Benerscke, et al., NewYork, Sp
3. Carltor, W. W. and Hunt, R. D.: Bacterial Diseases. In Pachology of
Laboratorv Animals, ed. by K. Benerschke, et al., New York, Spring-r@rffig"
L978.
4. Shadduck,J. A. and Pakes, S. P.: Protozoal and,Metozoal Diseases. In
Patirology of Laboratory Animals,.pp
ed. by K. Benerschke, eE a l . , N e wY o r k ,
160!|-1607.
5. ftretana, H.: Coccidiosis of the liver in rabbics III:
Experirental scudy
of the hisuogenesis of coccidiosis of the liver.
Arch. Pachol. 5:5L6-536, 1933.
TIT.ryIttYP. O.NEILI
Regisrry of Veterinary Pachology
DeparcuenEof Veterinary Patnology
- 5 -
Histories
AFIP !{ednesdaySlide Conference - No. f0
25 Novesrber1980
Case I - 80-331 (AFIP L757304).
sture, four went off feed and developed
derma-Effiwo1len
I14h nodes' anrdhaci bfood in cheir fqceq. An enlirged kidney
was palpated per rectLm in one heifer. AII four cattle died within cr,vodays of each ocher.
qqse II - urunrked (AFIP L755277).
ZE$reek-oldAirdale puppy frcm South Africa.
Foqr
pups died frcm a litter of eiglrt. The trrps died r:aiidiy shoring vague AnS signs.
Case III
- PL D18075 (AFIP L666524).
-year-old spayed female poodle. She was
euthanized following the-onset-of dysoneaand cr-iilt
nerve deiicits.
RadioeraDrrs
shotr a mass in the anEerior rediastinym.
signiiican;-iinCi
gnly
rg
;-;;E;6"y
was a 4.0 csr diaeter mass extending frm f.he
the basl of"rtre Gaic-6-;#
-'
of the anterior sEernun that was nuftinodular and mrcGd
"iga"hmefit
;4.
Fiion and dark
CaseIV - Wt{80/729-2
were distribured to bait rabbics in a paddock
including cows and calves. T\docalves were foind
'T
deadthe next day, anda cowandcarf (80/729-2,3)diee 3 a"irlit"r
-er
after baiting.
u"iiine. The
contafnrng30 head of cattle,
oemerobservedthe animals"drop deaa;A'rtr"" i"i,iaiy-ui*i'"1
$tasgeneralized congestitrrof visceral organsand'sJb6pi""iai"f
-d*;t-h!;;.
auropsyrhere
TI}oI}il P. O'NEILL
Registry of Veterinary pathology
Deparuneit of Veterinary pamology
Resulcs
AFIP l^Jednesday
Stide Conference - No. l0
26 i.tovemberl9g0
Case I - 80-331 (AFIP L75130$.
sture, four went off feed and developed
qermat_i.tis, swollen lyuph nodes-and
had btood in rheir feces. An enlireed kidnev
-;-f
was.palpared per lgqlb'f in one heifer.
All four carEle died wichin crâo?ay, each other.
Contributor's
is&
r'rqlrlEnEs: Abundant quantTElE ofEiiy ) poisoning.
vecclr weie found growing in che
renEry rc nad been introduced
-Ttr"-*,r.t"-r
to the farn the pieviouE y".. i'a road
of rrtreat
farmlought frqr another fapm.
ert-rn o.î
rw
r!.^ ^^---J--._
r
year
i:
BF",HI;;,3ffff#*I
l:#*H:,*:*,"n4^.:_r*
TtrewtreacF^;t-pF;i,ou"
gror[-il;-cdi*i"rin"'#
:H"ffi;?"ho*L':iff
::1":':li:!:xp:-iiG_ry;"f,"$#iir
Si"!fr!r.l;":il
:LTit'Jl*
$f:ffi*Jffi:
skin, ffmF*"TiF.ri:ff:.
;":hi!ffijFi",.?'ffl,'I"ilfi
kidney,hearr,iw,it.iili;il;rffi;d;"fi;IJ;i?#::
"rop
nf,'t
narvescaDle, consequenclv rh
affecred field was converred ro ;;;;;:''i.j'",I1"
the
pasrure
grazed
by rh6
rhis snrino crncc mzr -j^-^-^^!?:tyre;
"eiecdJ-""lcr"
seenin
granulomatous, m.rltifocal, mild to
Tit:r,
rrcderat-, sp
z) nyperplasia, lyuphoid, diffuse, rcderate,
spreen.
' Etiologic Diagnosis: vicia
villosa roxicosis (plarr toxicosis).
Etiology: Vicia villoffCrents:
ftiaftFffi[oAfc
diagnoses lrere reached after considerable
discussiqt' Ttre
!
etioroeic-Ei"qto"Tr*"r nol-ai""ro""a-*[ir-Ii.".
presenrarionof
chehistoloeic findings-" meTb5oriri-or-'rn1l.."na."s
proposed
an infecrious
etiologic alasngl-ii d;in."""iyloq"ci"iioriJ't-uoui'e
marigiranrcararrhal fever as
their pre-conference choice. "e
few
photosensitizatiqr and skin leJims, Jitr"tJ-ri"ced plant roficosis wirh resurtanc
ila-;dii-*r-relare
th;; clinicar signs wirh
the histoloeic lesions in Ehe spleen.-hr
,-"pori"i-Jor"rving
giant cells
with rnrltipre nuclei scattei"a-'cr,i"iiinoli-tilJ-fection
"tt*d"""
oi splenic cissue
in variabre
n.ders deiendingon their-particur"i
felr
rhere
wasan
increaseii the 6r-oer oi-rffito"ytic celi"-6o*,
"""ti*.-.;-i;;."5ffi;
in it.-,rilIJq,o red pulp, bur
this was considered dependent'on,'tn"
particrpangs
who rrrere
G animar.
familiar with this cqtbilion-ii'uovin"E"g"ôi
r"rt-ihe
for hair vetch misanigg ina not T.B. or
_resionswere quire characrerisric
M.C.F:- IE was poinred-our rnaE rhe
lesions lacked
of chelubercurus-granulma
i1'1-i1eiE"t'?oFpt"nce
wich casearionof
checentral portion
lurrowrdii by giant c"rrs anaproliferatirlg fibrous
catanhal i",l.r-*", consideredan urlikely Eause coruEc.ive
liryy'
Flig"tqtq
wich che
absence
of vasculicis andthe presenceof rhe .;i.&;ffietLh.
celrs.
Gianr
cells
are reported only in i.is5ue culture -J *t in
the narural disease. other
lesiqls obseivedgtottiy
i:. otg*.di"iiy
are
greyish corored nodules in
ryittr-tt
the kidnevs and
ThA re;ic';--in'tt" tiirey mr"r-u. differenciated
3prenal-qlgas.
from M.c.F., rvuphcra*E uasr coast Fever
<i" ai.i"li]':fri$iiogicarly
rhere is a
mixedcellular- iirfiltrate consistig oj
i)rrp&;res,
macrgphages
and
gianr
cells in
these Eissuesr*rich mayin soe-casJs u" cdeusfo
wirh m"rigrril- ryuphma. A snorc
discussiqr follq'red concerninfthe clinical
signs observedilith tr,i" roxicosis.
Pnocosensicization and dermaticis are reporced with chis and ocher menb:is .ri Ir+
genus
Herolytic disease also is boserved wicn scme rembers of cris g,enu.;.
'fne Vicia.
bloo?l-fi' uhe feies of these heifers was attributed co a local toxic etf5ct i'r
the G.I. Eract.
ContribuEor: New Bolcon Cencer, Pennsylvania Scace University,
Roadl%rrA?TE-Suare,
PA 19348.
382 i,l. Screec
References:
veEcn Pascure.
@,R.J.eta1.:AdiseaseofcacElegxazinghairy
JAWA 148: 804-808, L966.
2. Surith, H. A. et al.:
In Veterinary Pathologv, 4ch Edition, L 9 7 2 , p p 8 8 5 - 8 8 5 .
Case II - Unmarked(AFIP l-755277).
pupslilidfEor
a litter
of eight.
ek-old Airdale puppy fror South Africa.
Foui
The pups died rafi?tiy showing vague CNS signs.
Concributor's Diagnosis & Couuents: Canine cerebral babesiosis.
to che young age of the pups. Alchougn
congenital infection has not been ruled out, the general opinion is chat Ehe pups
acquired the-infection via tick bites jusc as older dogs. These pups rusE have
been infected soon after birch.
AEEendees'
is & CortrEncs:
:
brain, Ai
Erythforyiic parasitemia, capillaries,
cerebral corcex,
EEiologic Diagnosis:
Canine cerebral babesiosis.
EEiology: Babesia sp. probably B. canis.
C6spnts:
lbere was unaniuDus agreement with the contributor's
diagnosis and
cmmnts.
A11 the parEicipants reported observing nLrrErous intraerythrocycic
procozoal parasites within the capillaries
in che brain sections. Differential
etiologic agents listed included Heupbartqrella canis, HepaEocystis sp. and
Trypanosmra sp. Other histologic@
ffiere
neuronal
degeneracion which was not considered a prorinent feature and perivascular
hemorrhages wbich $rere present in a few seccions. One of the cqrtribucors $ras
present at the conference and discussed the clinical
and histopathological
findings
of this disease in dogs in his experience.
Clinically
the signs are a marked
fever, dry cough, pronounced anesr:ia (L5-25% PCV), icEerus, heroglobinuria,
splenouregaly and hepatoregaly.
Btood srrears may reveal a 60-707" parasicemia of cne
red blood cells.
Grossly the spleen and liver are enlarged and dark and Ehe krdne;a
also appears dark. lbere may be generalized icterus and suMural hemrrhages.
Tne
lungs may be heavy and weu and the urinary bladder usually contains bloody urine
(red water).
Histologically
there is puhnnary edeura, congesEion in nunerous
organs, and hemcsiderosis in the spleen. InEerscitial nephritis and cenErilobular
hepatic necrosis may be present.
Histologic lesions in cne brain include
congestion in Ehe cerebral vasculature, widening of rhe VirchcnRobin spaces, and heurcrrhage in the midbrain and base of che sulci.
Diagnosis of
che cerebral form is made aE necropsy by observing parasitized RBC's in cerebral
capillaries
in Giemsa stained brain snears, particularly
fron the hippocampai
region. ParcicipanEs agreed with the contributor's conrEnts concerning the route
- 2 -
of infection in this animal. The.prepatent period
for this disease is reporced co
be between5 and 24 days. corgenital-cerebral babesiosi"-t".-!*n
reporced in
newDorncalves (2). tn efrica-trea@nt
of chis disease in dogs is direcced ac cne
acute srage of rhe disease bur nor aE eriminarion-ot-d;ccnprere
elimination of the parasite rendet"-ir,.-*i*i-susceptible p"r;?;".
to
subsequent
infeccion
as the.iuurirnity wanes. A low level-ot p."."l"ri"
il-;;;f";iei
wirn resulranr
r premunity. splenectotrryresurts in ross'or pieuuority.
contributor:
onderstepoort veterinary ResearchrnstiEufe, onderstepoort
0110,
RepuElT-68-5oiEh Africa, ina-ir," ueparueni ;i-- Zoonoric
Diseases,
Armed
Forces
Institute of pathology, Washingron,rc ZOfOO.
References:
1nrom-rl*'F'
andKennedy,
P. c.:
rn Pachologv
of Drcsric Animals,2ndEd.,
2' De vos, A' J. et al.: Cerebral babesiosis in a newborn
calf.
J . V e t . R e s . 4 3 (Z ): 7 5 -7 8 , L 9 7 6 .
onderscepoorc
case III - FL oZSOZSlAEtp
L666524).
-year-ors
spayeo
poodte.
temale
She was
euthaTaFFo[cx,ring *re-onsel-"f q"e"6;;;-;rdi;l-";r*-iefi.cics.
Radiographs
showa mass in the anterior urediastin,.m. The only
aE necropsy
was a 4.0 cur diasprer mass extending qrm, itre Uas6of"cne
"igriii;;;-irnorng
treait- to che aLtactsrenc
of
anEerior sternurn that was uufrinodurar ana olciri"J;Jil
and dark red.
.the
contributor' s DiaFnosis:
Anterior MediastinLrlyuphoepithelima.
AtEendees'
FDEpnoroglc llagnosls:
conenrs:
Thymra,
anterior
rediastinrm,
agreerrnnr
wirh rhe
yr:_genep-ar
poodle, canine.
s' ai"gnosi",
T:::
alchough the preferred-rniprrologic"diagnosis-was
"*rriuicoii
cnyma-as
classified by the
cip#.;- i t";A ii;iffi..ryfu;;;;.ccrne
and
H9,
I lI ^-?lII::"*iil_rL
lheApaiti
undifferenriaEed
carcinoura.
f"w oir,"i" Li;;;J
piriJur"'frili,rr.ory
nediastinitis. Histologic features discusJ"a-wrb *re mix.d-;al popilrarion
of
lynphocytl"
cells 4i"!, generatedsucn aescripiiu" ceru6 as mixed
"qd.epithellal
epithelial and
lyryhocyti" .*d predorinEiili-gpi.r,;1i"1-;;-;i;;;
cerr rhymra. A
ffl,pttlicipants- ripollea they
bbsetu"aH".i"itr
in
rhe
rissue secrions,
while others observid multifotal necrosis in the "orpuscles
tuunr. rn a recent publication it
wasProposedchat EheHassel's corpusclesoriginate frqr ihe
nrmonuclearphagocyticcells (3). 'The corbinEaryrpt*yril-*i "ii"uf"ting
epirherial
cotrponentsare considerednecessary
the
diagn6sis
oi
thytmma
nt icn helps to
for
i'^?:?plu?o,f :q'. purLryuphocyti-sarccma
andhisriocyric
1..::llg:i:L:h
rynPnosarcoma. Discussiqt follotoed
concernirlg-the
association of cnyumn and
Myasthenia gravis and the suppression of acerllcholine
Ehe neurouscular
junction and the occurrence oi chese cwo conditions
"p;k;-";
in the
carine (4).
concributor: Experirental pathology Laboratories, rnc., p. o. bx
HernilloilTA-ffi7O.
- 3 -
474,
References:
Neoplastic diseases of Ehe hasncopoteEic
l:rrefi
W.F.H. & lrbckey, L. J.:
8u11. Wld. HlEh. Org. 50: 2L-'34, L974.
and lyuphoid tissue.
and heunpoiecic cissues. In
Tunprs of the lphoid
2. lbulton, J. E. et al.:
Turrcrs of Dqrescic Animals., 2nd edition, University of Calif. Press, Berkeley, CA,
3.-Blau, J. N.: LetEer Eo the Editor. Tne Lancet, Jan. 20, L979.
4. Parker, G. A., Casey, H. W.: Thymas in Dmstic Aninals. Vet. Pathol. 13:
353-364, L976.
L76L487
Case IV - Wtl 801729-2
were distributed to baic rabbits in a paddock
conta
head of caEtle, including cons and calves. Trro calves were found
Tne
dead rhe nexr day, and a cow and calf (80/729-2,3) died 3 days after baitirg.
At autopsy there
o{ner observed the animals "drop dead and then rapidly bloat".
heuorrhage.
al organs and subepicardial hesprrhage.
lras
consestion of visceral
was generalized
seneralized congescion
Cqrtributor's
necr
1080 coxicosis.
Diagrosis & Cments:
Multiple small foci of myocardial
rs, wittt histiocytic infiltration.
im and necrosis, mrltifocal,
mild, heart,
bovine.
Etiologic Diagnosis: Fluoromroacetate toxicosis.
' Etiology:
Sodiun mrofluoracetate/ fluoroaceto.'ride (1080) .
CrenEs:
the attendees agreed with the contributor's rcrphologic description
and etiologic diagnosis as based on Ehe histologic lesions and case histoJY. A fetr
preferred i mrphologic diag,nosis of acute mrltifocal necrotizing nyocarditis as
of histiocytic/macrophagic cells in response
iupporred bv rbi obvllous infiltration
to-Lhe uryocbrdial necrosis. Each diagnosis was considered equally acceptable.
etiologic diagnoees included water hem.lockand Japanese yeit
Differential
toxicosis. The lesicrs present are considered nonspecific and_diagnosis is
sErongly based on tlre clinical history, gross and microscopic findings. .Other
lesiois- associated with this rodenticide are subepicardial hmrrhage srd visceral
herorrhage in cattle and pulnonary edemaand congestidr of the liver and_ki$gf.
hgs, caEs alrd pigs may bircrc inilirectly poisoned through cons-Lqtion of 1080
infoxicated rodinEs. Clinical signs in dogs and cats sust be differentiated frm
those of strychnine and thalliwt poisoning.
Contriburor:
Regional VeEerinary Laboratory, Wollorgbar, N.S.W. 2480 Auscralia.
Refererices:
T. T-uekff
L976,p. 233.
B. et al.:
2 . S m i t h , H . A . et al.:
Clinical
and Diagnostic Veterin
In Veterinary Pathology, 4th Editiqr,
, 2nd Ed.,
L972, p. 823.
TII'OII{YP. O'NEIII
CapEain,USAF,BSC(VC)
Regiscty of VecerinarY PathologY
Deparmrt of VecerinarYPauhologY
- 4 -
Hisrories
I}
AFIP t{e&tesday Sfia. Canference No'
1980
f0 Decesber
CaseI - 773U-:
fou*I
#of.
tntl:n.::t
a series of feral Californi" ry"Lig:
t"1'*
tT:',.,"l
-"rti".--ii'r"t
i".
u"'"q'i"a'L1""'il:'ils:i;'-e"*.til; *: lt 3gl?f
febrile - and had signs
*q 'T,9ui::-'.,.,.,*.,
rvr'
SCrended (xl a Los AngersIl vr'\nrLt
- *it"f abqt 9 urclsPI "g"tteo daysin captiviEv'
ri dia after E
;i ffiti".CaseII - 61:tnO -.
L727493
CaseIII - 80-2858-
Hrstory:
tissue suspensicr, ten mrchs prior co
n57437
iffixed *Ja"gbreed
ff13::d-:g
wam
febrile"with.inlarged
-i;ii-;.ditii:l *." Pl:,99-.:::
" ffffi;"i;[6:;;:Antibiotic EnerPaYwas
il8'S^Lil;sfi
l!3Hlfl;
-u[:r;'"*y*::'HrffiTilJ
ffiH3=Li?"l;;iil-:*111i1*,
:l:ffu13"?
ifi
.Si.ffi"L*i*o''H"
thedoghad
H:*:i* [3:; ffiH: fl',,f;iilTi#'d!"ffi;;I iri,,i""iii "ormar.
nevet been eexuallY active'
arxi3
grqsgrted-:lth a history of dyspnea
week' The
ane
'
i"t
inriod-oi
td-pi""Lt
.,a,-discharg"
ilO
*hilil
nas
m.lcorturulenE
_--+
ffi5i-;;,
.uit-ir"a--a
perforred.
i necropsy
I ' a b o r a t o r v R e e g } l s : - S i g + i f i c a n t qii*r,
r q ? l errtrit"
s i o nmass
s w e rchat
e l i P iinvaded
t " g t o c hadjacent
elefcnasal
tap.E;
cavi@reguur
;;sG and filled the entire cavitY'
TITOTIil P. O'NEIIJ"
Caotain, USAF,BS(Vt)
of VlgerinarY PathologY
n"ii"c"i
of VeterinarY PachologY
D";"tE#"t
Resulcs
AFIP Wednesday
Slide Ccrference - No. ll
l0 Decenber 1980
Case I - 77324- (AFIP U56983).
a series of feral California Sea Lians wtrich was
foundErEffi?led or-ra Los Angeles County beach in the early srrer of 1978. It was
an aninal about 9 ustths _of age, and was quite thin.
It was febrile and had signs
of pneulnrnia. It died after a few days iri captivity.
Co'ntributor Diagnosiq I Cmnnts:
Acute Viral Hepatitis.
had noc been slen previor.sly in chis area,
nor rePorEed in the literature.
There is acute necrosis drich is foiltifocal
throughou! !h9 liver secticr, and affecting large areas of se lobules. There is
a sParce infiltration
of neutrophils and mnqructear inflFqretory cells at the
periphery of the necrotic foci. Nrrerous intranuclear inclusion bodies are present
in the hePatocytes in the affected areas. Attespts ro isolate the virus weri
unsuccessful. Ulftastructural examination deusritrated virus wtrich had the
rnrphology and size of adenovirus.
A t t e n d ee s'D
agnos
tis, necrotizing, acute, diffuse, severe, liver,
California sea lion.
. Etiologic Diagnosis: Viral hepatitis.
Etiology: Suspectedadenovirus.
Cosrents: Tbere-!{as genelal agreeuent with Lhe contributor's diagnosis and
ccstrEnEs. The distribution of the lesions was cqrsidered centrilobulSr and
midzonal overall but several coalescing foci obscured the histologic picture.
Generally few inflarmratory cells were 5bserved and these cqrsisted of'Plt{'s and
rnacrophagesas reported by the concributor. Several accendeesreported basophilic
intranuclear inclusions surrounded by a clear nuclear zqre but these *ere raie.
The-majoricy-9!.the inclusions were a smrdgyeosinophilia and occr.rpiedthe encire
nucleus. A differential
etiologic agent considered-by several was-herpesvirus
infection based on the inEranuclear inclusiqrs.
It was pointed ouc that inclusions
are not specific for viruses. The histological appeararre of the tissr.p was
reuriniscent of infecEious canine hepatitis.
ffre lesions were not sqggestive of
leptospirosis which is endeur:icin sea liars.
The pneumnic lesions observed
grossly may be related to lungworminfestation, probably
Parafilaroides decorus
-interuediffi
which is found cmrn'nly in wild sea lions. The
teleost fish, Ehe Opaleye. the worms found in the stmnch were probably
ConEracecuur
gg. and/or Anasakis g
Concributor: los Angeles Cornty Departrent of Health Services, Ccqarative
MediG[-EiitGErinary
SErvices, L2824Erickson Ave., Downey,CA n242'.
Reference: Britt, J. 0. et al.:
lions.--fAVFh-175: 92L-9?3, L979.
Acute viral hepatitis in California sea
Case II - 65-L970 . (AF.IPL727493>.
tissr.|e suspension, ten IIDIrchsprior to
necropsy wlEtr nass in flank area.
Cantributor Diagrrosis & Cmnts:
viru6-s@s:i6h.
Fibrosarcwra induceC with bovine papitlma
Ilasters respord with fibrosarccma in the subcutis or brain fro
Crents:
locaTffildtion
of bovine-papillsna or deer fibrcna virus. About 10%have late
pl-wrary retastases. Wtrena ffank tL@r is indr.rcedas in this case, there is a
marked stimulatict of pigrent laden cells rnroally concentrated in this area. The
pigrented cells are principally reIanocytes though nast cells also are found in
these fibrosarmas.
AEtendeesl
:
FSffisaRc@a,
skin,
flank area, hareter.
Etiologic Diagnosis: Viral indrrced fibrosarcma.
Stiolog5l: Bovlne papillma virus (Papovavirus).
Crents:
lbe mqrphologic diagnosis, etiolqgic diagnosis and etiology were not
feacbed until after discussidr and disclosure of-the 4gent. Ttre najority of the
participants favored a malignant relanma, spindle celf type ae tbeir pr6ccrfererce
chgice. Tttis was based on the presence of the relanin pigment in large areas of
the ctlDr.
The @urlt of pigrent laden cel1s varied frm qle sectiql to the next;
soe had l.arge areas of mature fibrous connective tissue with little
pigmrt wtrile
others had considerable pignentatiqr in their sectiqrs.
Junctional activity was
absent. The two principal cell populaticrs present were fibrocytic cells and
unlanocytes. Several attendees also reported observing nrrerous mast-like cel-ls
t{ith cytoplasm:ic granules in less differdntiated areas of the tLmr.
The ctrrcensus
of opinion was Ehat the mass appeared malignant although micotic activicy was
minimal. Other viral agents which have been reported to cause fibrosarcoas in
hamsters include SV-40 virue and avian adenovirus (CEI0).
Contributor:-
Driv6lE?lffitlrl
Departmentof Pathology, University of Wisconsi.n, 1655 Linden
5gto0.
References:
T-T
ffi',
R. A. et al.:
the Golden Harnster. Iowa State University Press,
Ares, Iowa, pp f00-101.
2. Robl, M. G. and 01son, Carl: 0ncogenic actiqr of bovine papillma virus in
harnsters. CancerRes. 28: 1596-1604,1968.
-z-
Case III
- 80-2858 - (AFIP L757437).
ixed breed male dog was presented for
Iethargy, anorexia and polydipsia.
The dog was febrile *iih enlarged warm
testicles
and an enlarged left msrdibular fprph node. Antibiotic
-herpay was
inscituted
for 2 mrths and castration recsrmended. The or^ner delayed che
castration for 5 sstchs at which tire the dog was clinically
normai. Ttn dog had
never been sexually active.
-@t"'PcV38%.Totalproteinl0.6grd1.824rorrocyces/ul.
Ser@
Brucella canis wa3 l:4O0.
Cqrtributor
& Coments:
a) Epididpritis
and orchitis,
dif
Cquents:
An etiologic diagnosis of BrGella canis orchitis
positive brrrellosis
titgrând the degeneEEli6--a'n?lEFlasmatory
was based on the
changes in che
Lesticle and epididyuris.l,2 Organism6were not seen with Gram3ain.
TtE cause
of the intersEitial
intersEitial cell hyperplasia (which was ncre diffuse and mar:kedin rhe
contralateral tesricle) is unkncrrm. It has been observed in man that intersEicial
ce1l populations-yqty inversely with the spernatogenesis.3 Widespreadnodular
hyperplasia and diffuse hyperplasia of interstitiil
cells are noc tnrcmn in
aErophie testicles in anirnals.
Atendeesr D
c Diagnoses: l) 0rchitis, chronic, diffuse, mild, testicle, canine
2) Epididynitis, chronic, nulcifocal, mild to mderate, testicle.
3) H#rpGi;,
interstiCial cell, diffuse, rnderate to severe, tesEicle.
Etiologic Diagnosis: Orchi-epididymal brucellosis.
Etiology: Brucella carris.
Cqurents:@eral1yagreedwithtlreco('i:riilutor'sdiagnose.sand
cqnents.
A variety of rnrphologic diagnoses lrere submicced prior tc t6e
conference t^lhich either incorporated the three separace entities or listed chem
separacely. The bistory was cqrsidered quite suggesrive of and the lesions
consistenc htiEh brucellosis in the dog. Discussion of the lesions included the
severe tubular degerreratict, the lyrphoplasmacytic infiltracion
of che inrerstitiun
and the diffuse inters:icial
cell hyperplasia.
Several atEendees reported
observing an increase in fibrous connective tissue characEerized as i r:hickened
eosirnphilic
fibrillar
strmra surrounding scrne degenerating tubules.
Whether this
was absolute or relacive was not resolved.
The direct caGe of the seniniferous
tubular degeneratigq *qt postr.rlated to be either directly related co Ene baccerial
infection or possibly due to an increase in local tissus teryerature due to
inflamnation.
The intersEitial
cell hyperplasia was consideied real and related co
Ehe tubular
atroohv/deseneration
of negacive
nconrivc
fanedhenlr
atrophy/degeneration via
Ehe_tubular
via lnct
thik 6f
feedback inhihiri.l''
inhibition.3 3 Tlra
TLre
jrfLanracory cell infilcrate
was minimal
was
minimal in this
this tissue;
tissue; apparently
apparently Ehe
maiority of
Ehe majority
the inflarmntion had subsided as based qr the history and hisrotogical
histolosical findings.
findines. A
shorc discussion followed concerning the innnnrity to Brucella organisms. CelI
nediated irnnrnity is the prirrcipal host reaction and form of eliminaciqr of Ene
organ_isms. Htspral antibody production also is irportanc in the elimination of
brucella organisms. Stains euployed in the demrnstration of Brtrcella sp. include
Grffi's, especially the Brown & Hopps nrdificacion,
and occasiofil-flll$,ntS sr.lver
scilin.
In hurnansthe organisrs sequester in the bone rarrow and ii atteo;pts
- 3 -
to isolate the organism frcrr the blood fail, culture
should 6s 6gg"mFgl6. In dogs bacteremi4 my persist
vaginal discharies frm bitdhes wiEh recent-history
prfismpe of the-ma1e may yield organisms on culture
redia.'l
Cqrtributor:
of bore merrow aspirates
for several mnths. Blood,
of ahorciql, testicle and
with prescribed culture
DeparmnC of Veterinary Pathobiology, ltte Ctrio Sfate [Jniversity,
coh-ffi-tiFlCffi9ro.References:
l,hle dogs naturally infected with Brrrella canis.
l. n. et al.:
ffire,
1969.
1352,
155:
JAVI{A
2. Gleiser, C. A. et a1.: Pathologic chcrges in dogs infected with a Brrrcell?
Lab. Anim. Sci. ZLz 540, 1971.
organism.
Atr. J. Path.
cell grohrthof the testicle.
Interstitial
3. Warren, S. et al.:
L943
19: 307,
4. Dbore, J. A. et aI.: EpizootiolOgy, diagnosis and control of reUg
canis.
JAffi 156: L737-L740, 1970.
Case IV - 4-7-8L - (eflP U58838).
presented with a history of dysp-neaand a
mrco$ffiIfiE nasal discharge which had been present for a period of one week. Ttle
anirnal was euthanized and a necropsy perforred.
Laboratory Results: Significant gross lesiqrs were limited to che left nasal
shapeE, firm, wtrite mass that invaded adjacenc
cavi@reg[lar
tissrr and filled the entire cavity.
Nasal adenocarcinula.
frm a flock of urkncnn size.
tumrs have been rePortd frm this area.
Cantributot Diasnosis & Conents:
ettmturbinate
AEtendees' Diapnosis & Crents:
Endemic
inoa, nasal cavitY, sheeP, ovine.
Codentsl ftrere was total agresent with the contributor's diagnosis. A fetl
participatte added sr.rchdescriptive terms to Eheir diagnosis as papillary, -or
several atEendees listed adenma
adenocarcinma. Differentially
iapillo-tubular
6aied qr the very urriforn well differentiated cells cqosing ghs mass. Etiologic
diagnoses listed by several attendees inchded endemic nasal/ettmturbinate
adeilocarcinora of sheep or simFly viral induced nasal aderpcarcirma of sheep, Tbe
Participantg fgvol-rng a v_iral etiology
proposed etiologic agent was retrovirus.
particles
mgphologically similar to a
ir.
whictr
viral
recent
iepori
a
iicid
visna-maedi virus-were detected in trmr tissues.Z In sm sections 3 ernn!]
anunt of bone $raspresent and felt to be reactive bqre.versus pre-existing.
Several participantb reported the presence of m.rltifocal squmus retaplasia wichin
Ehe tunci. Coniiderable variability was reported in che mitotic rate of the cumr
cells. the cell of origin of this tumr is rpt precisely knonrn;it oulC arise
- 4 -
fror either unrcosa|.gniqefiq gT.Bowman'sglands in rhe lamina propria of the
Ilrrcosa. A mild i:llrytory,infiltrate
conEisting of neurropirifi, iyoptocyies
sorlE llErcrophageswas-observed by several attendeeS. These nioplasms are noc and
rePortd to metastasize but are, locally invasive *a r"y-progrE"r inEo Ehe cranial
vault with resultant neurological lesions. Differenririfi-UE""a
o.r rhe hisrory
oesErus ovis parasites mtst fu cqrsidered with greenish nisal discharges in-srr'eep.
Cqrtributor:
Ibrsh Veterinary Laboratory, p. O. Box 997, Bozenan, MI
59715.
References:
fEintS"-S.
et al.:
Neoplasusof the olfactory mrcousmembraneof sheep.
Cornell Vet.-51: 97-LL2, 1961.'
2- Yoneurichi,H. et al.:
Intranasal tuupr of the ettupid olfactory sucosa in
sheep. Am. J. Ver. Res. 39: I599-f606, Lglg.
TII'Ofl{Y P. 0'NEILL
Captain, USAF,BSC(\IC)
Registry of Veterinary pathoiogy
Deparrrrent of Veterinary patholbgy
*F*PLEASE
TUIE TTIEFOLLOÎIrcCI{A}GESFORCONFERMGE
#10*t
*Addition:
-Er.#10
Case II --- Add to end of Attendees' Cmrrents:
rf treacuEnt of the acute disease results in s;erilization
the animal is susceptible to re-infection.
of infectiqr
:k*Correction:
-6;r#10
Case III
-- Attendeesr Cmnrents:
Sentencebeginnirg - In a recent publication.
Should read - Hassel,s corpuscles function as rmnonuclear phagocyEic
cells of the R.E. systen.
- 5 -
HisEories
AFIP lilednesday Slide Conference - No. LZ
17 Deceder 1980
Case I - A-18946 - (Ar:P L667LU).
Siberian Husky presented with a history of righc
forelffrE--ffiness for a few days. Ttre clinical diagnosis of panosEeiEis was nade.
the dog was tTeated with steroids, analgesics and Vit'nrin C. A few days later, the
dog was presented nar-anbulat*y, with tetraparetic cervical spine pain, ard upper
Cervical cord neoplasm (glima) was the
rctor neuron signs of the hind lids.
clinical diagnosis.
Laboratory Results:
CSFanalysis, Heoatolq6/ and $,IA were unreugrkable.
CaseII - L3747- (enP L7Ln77).
f a ma1e C5TBL nice out of a group of l0 chat
aiedTEtilet'fy. Ttre survivor was dyspneic. Sixteen of 20 nale ICR mice frm the
s€re roqn died at the src tire.
Laborqtory Xesults: Bacteriologic and parasitologic exans as well as virologic
tite@
rCR mice weie negacive.
Case III
- 80P465- (AFIP 1758396).
dulf;er-5d€l= colic,
old.
of paint parentage, with clinical signs of
was presented to the CSt Clinic for euthanasia. It was one day
Case IV - 80/0623, H80/775 - (AFIP L757M3).
d Angora goat kid. The aninral was
Esrc
weeks
of observation it was unable
in
sternal
rectdancy.
Dtrring
the
found
The rid
walk or rise but resrained alert and continued to eat and drink.
withdrawal reflexes trere normal. The country of origin is Australia.
-
#
-
TI}OIIfl P. O.NEILL
Registry of Veterirnry Pacholory
Ibparmnt
of VeEerinary Pachologr
Resulcs
AFIP WednesdaySlide Conference - No. Lz
17 Decenber 1980
L667L12
ffiiE Siberian Husky presented-with a history of rigltt
Ttre clinical biignosis of panosteitis las made..
days.
a
few
for
CaseI-A-18946for
Ttredogwastreated wich steroids, 9n3lge9icsand.vi!19i-t-c;-l,Ia-9?I" k:"::i:"
aotrffi pi.""ot"d-"od-,buf?t9El:'with ietraparetic,cervi3l tqi1r9Êi":i9.PP"t
- - 9 ' - - -
F - - -
:
tr13rorneuron slgns of the trind ii$s.
clinical diagnosis.
Laboratory Results:
Cerviial
cord neoplasm (glima)
was the
CSFanalysis, l{ematology and $1A were unrenrarkable.
Cavernous angicoa.
Cqrtributor Diatrrosis & Cqrents:
Cm|Irents: GavernousarlgrcrfTFT vascular maffSrmatiqr in the brain or spinal
cord reoorted in hrman patiEnts. The anmaly is characterized by the presence of
btood vissels lined wirh endorhelial cells supporcd by collagernLls connective
tissue. These charrrels are not separated by rer.rral tissue.
AEtendees'
, caverrpus, spinal cord, Siberian husky,
canine.' (Vascular rnalfornation)
- r
Corents: Ttre diapnosis listed was preferred as classified by [,][{O.r
Histological fbatures c@nted
tments.
Agt;tees-"gi""d--*iitr-ffi.-cartriburor's
vascular spages,-Ehemlltifocal acute and
*-rl"t-Ltr."G.g.
""aoiftefiaf-lined
in the cord, and the neurmal and axonal degene-rqtict present.
subactite hmrriaee
in the .qt"iliry_*:I-T?:8""
obsErving focql glriosis and satellitosis
S*--po"ted
beneath the endochelial
located
naterial
of the cord. Th;-;;i;ophiiic"fibrillar
together
collagen
with
cells was felt by many d be corpacible
-wich scrlE sD(rth
seconda:y-t9 tE
coGidered
weie
cord
in
the
chanles
dt" n"irr*rl
m.rscle cells.
based qt
congerital
to
be
believed
was
lesiqr
Ihe
ih; anornaly."
expansile *c*"-of
th! age of the animal and Che features of the lesion. It was considered
wirh relangectasis because normal neuropll ras not q--1e,s-e1t-bebfeen
i**ficible
the viscular spaces a1d-che blood filled vascular sPaces Llere very latB":_tf ^,
with sclerosing hemangima-of man.based qr the small
lesiql also was i".."e"tible
lotl nr.der of vascular
aptnt of cormective tissue present and thJrehtively
were considered to be of
lesion
this
in
involved
ctanners presenE:z Ttr;-Fslls
venEral suMural origin.a
ConEributor: Ttre Animal }edical Cenrer, Parhology Deparmnt,
HeryY6:T,-ffi0OZt.
510 E. 52 St.,
References:
Tuprs of the soft (l&serrchyroal)Tissr.res. Bulr- t*to 50:
ffi.,
L974.
l0t
--- -r 1 0 ,'t-leoplasm
of the Central Nervous Systeur. AFIP Trmr Fascicle.
,-.
3. ZakL, F. A.: Vascular malformatiln ,(cavernos angima) of the spinal cord
-1. Sn. Anim. Pract. 20: 4L7-422, L979'
-tordy,
in a dog.
D. R. : Vascular malformations and hemangicrrasof rhe canine spinal
4.
cord. Vet. Path. L6z 275'2M, Lq79-
- (nrtr L7L3077),
caserr - L3747
f a nale C57BLmice ouc of a group of l0 thac
The survivor was dyspneic. Sixteen of 20 male ICR rnice frm the
died-ffftIdty.
sarrp rocm died at the satrE titrE.
Laborqtory Besults: Bacceriologic and parasitologic exrrr! as well as virologic
tite@
ICR mice weie negative.
Contributor Diagnosis & CmEnts: Kidney, tubular nectosis, probably due to
chlo
Cmtents: There is marked necrosis and degeneratiqr of the cortical tubular
epithelitm with generalized sparing of the cuboidal epithelir.m of Bovmcr's capsule
and upper segrents of the proximal tubule. Procein casts in the tubules are
prmiirlnt.
These lesions ire typical of brief exposure to chloroform in certain
scrains of male mice. Investigalion showedthat ihloroform appatently was used in
the anrirnals' rom in ttre days before the deaths of these mice.
Etiologic Diagnosis: Toxic Eubular necrosis.
Coupatible with chloroform toxicosis in rnale mice.
Etiology:
Attendees' Diaprroses& C@rts:
, tubular, acute, nultifocal, sevete, corcex'
kidney, C57BLEDuse.
Cments:
the mrphologic diagnosis was agreed upcr after sKrIEdiscussiqr of
the lesiqrs.
Several atteniiees preferred tubular nephrosis as their preccrference
choice based qr the etiology.
Histologic features c@nted on were tbe acute
tubular necrosis characterized by hlpeichrmasia, pyknosis, karyorrhexis and
karyolysis of the nuclei in the involved cubules, tbe coagulative or hyaline change
of che cytopLasm of these cubular ceIls, formation of casts, €lod the presence of
small ntmbers of PI'N'g. We agreed that the distribution of the lesims was
restricted to the proxirnal tu6ules and that the cuboidal epithelim of the Borman's
capsule was spared. Differential etiologic diagnoses considered were heavy retal
The pathogenesis of the lesiqr was
and ihlorqrated hydrocarbon toxicosis.
discussed including, the apparent predilection for the proxirnal tutular epithelim
based on the high uetapolic activity of the cells and the role of androgens in
strain suscepcibiLity.r'z
Other lesiqrs reporced in chloroform toxicosis in mice
are nineralization of renal tubules and centrilobular facty change and hydropic
degeneratim of hepatocytes. I'tice differ frm other species of aninols in the
excreticr of chloroforur mtabolites in the presence of testogterqte in male mice
and in ovariectorized female mice treated with testosterone.J The strain of
uouae involved in this case is cqrsidered less gusceptible than those highly
susceptible strains reported in che literacure.z
Contributor: Division of Cqarative
of MAIilc:i-n€--IZO
Rutland Ave., Baltimre,
t&dicine, Jotrns Hopkins Universicy School
l,lD21205.
References:
T@r,M.etal.:ResultsofexposureofstrainC3Hmicetoctrloroforn.
Proc. Soc. Elxper. Biol. 83t 474-479, 1953.
Pathology of Laboratory Animnls, Sprirger-Verlag,
2. Benirichko, K. eE al.:
NewYork, 1978, p. f48.
3. Taylor, D, C. et a1.: lbtabolism of chloroform II sex difference in the
recabolisrrof (Cr4; chlorofonn in nice. Xenobiotica 4z (3) 165-174, L974.
4. Hill, R. N.: Differential toxicity of chloroform in the muse.
An n . N . Y. A c ad . S ci . 2 9 8 , 1 7 0 -1 7 5,L978.
- 2 -
Hiscories
Carference - No. t3
s1ia.
AFIP WednesdaY
1 January 198L
t
cynmlgus
urkey
frcm Malaysia, one of a
grouP o
'
CaseII - R27979 - .(['YLP,L72!287)
_-_-_^1 ^r..1
f f i e r-+i s S u e i s t r o d _ a c o n c r o 1 a d u I t m a I e F i s c h e r r a E E h a E d i e d
*re rai was fourd to have a r'ilateral
spontaneousry. lt-rr."ropty,
ArrP I7rB25).
a Imusc
ffined
om
abdorinal nass'
housed ac a
cephus) hoysef
(Qerglllecus ggp!gg.)
rcrrkey (Cercopithecus
rcnkey
hlsEory or a ptevrc'\ru 'Llrr
;"k"y;"d
unnkey hacl a historlo@=?::l-^
'+
-.,
to signs
rlr.'rrLlrp Ptior
LfsaLeu 4 mnthswas
l'! rv'
w.i tr"ated
call
the tail
bone of Ene
the bone
in the
fraccure.
g6s
snirnal
for ane day and
of severe diarrbea and seizures. Seizures cqtiin,rea
died.
z@.
t, feqale
infection
An infection
Case IV - 0070 - (AFIP U58837)'
i. cat. The cat was Presented co the referrilg The animal
depressibn, f,d icterus'
with the ccmpraint of *or.*i",
veteffi
died despite supporrive therapy and i'ras necroPsied.
A snall
At necropsy the carcass was markedly icCeric'
Laboratory Results:
Liver was
che thoracic cavity.
or.snEity ot scraw-colored tluld $ras present ln
aPpearanc€'
dnlarged and had a mttled
TIT'Ofi{YP. O'NEILL
CapEain, USAF,BSC(VC)
nes.istry of VeEerinary Patnology
Defurtui:nc of Veterinary Pathology
Resulcs
AFIP WedresdaySlide Confetence - No. lj)
7 January l98l
CaseI -27L78 - (AFIPL755279).
the cynuolgus mnkey frm Malaysia, qte of a
@ictrascffis,
groupr-f-Iffi
i@ity.
is&
Cqrcributor's
ion'of Capillaria
n macaquesfrmr SoucireaffiEllIThe
inc
are the urost iuportant
Attendees'D
eggs in the liver is often an
eggs are distinctive and
facEor in the produceian of the lesion.
is&
's,
granulomaEous,m .rltifocal,
rcderate, liver,
----n-ti6Foffi-os
is : Parasitic hepati t is .
Etioloqy: Capillaria hepatica.
pa;El@rts
agreed with uhe contributor's diagrosis and
CcmEnE;: Gffice
ilistribution
of che lesiqrs was quite rando. A
feit
the
Participants
c6rents.
ducts.
A few oChets felt there were areas
within
blle
few reported obseriing eggs
a diffuse vacuolar ct nge
all
described
and
of cenlrilobular necr6siE-m"rltifocally
Ttte
to mild autolysis.
due
have
been
within hepatocytes. The vacuolar chmges may
be
either
to
widely scittered pignrent in the Kupffer cells was considered
hennsiderin or maiaiial pigrnnt. this species of mnkey cournnly has chrqric
malarial infection drich- is refractory to therapy. Manyof the eggs lcre in
0ther inflenrracory cells scattered in these
mrlcinrrleated giant cells.
granulmatous lEsions were lpphocytes, plas:aa-cells, ffid eosinophils.
Cansiderable fibrosis accorpanied these foci.
The eggs were of the Trichuroid tY'P€, i.e., had bipolar plqgs-and were
and those that rlere rot degenerace
elmrgace.--1he egg shells had striatifirs
Other Trichuroidea with similar- eggs were listed.
contiined a singlE cell.
Trichuris eggs lre rnre ovoid and wichout striatiqrs in the shell; Tri.chisoqoides
Ts-founfin-Ehe urogenital systerr of rodents and its eggs nay F foud in Eisst-e.
Anatrichisma also is found in primates (i.e., macaques). It-lives in the nasal or
rmcostfepTtr'eliun of the oral -avity, md its eggs are passed in feces. Ttrese
eegs may be differenciaced frwr C. hepatica since eggs of Anatrchisomacqtcain a
larva.
ltre life cycle of Capillaria heaqtica was reviewed. Rodents are the usr.sl
man) also. may serve as final hosts
final hoscs but other ffifs-@..g..-*r:ffieys,
hltrenerbryonaced eggs-are ingested
since Ehis pardsice is not very host specific.
make
their vtay to the liver via che
and
by a suiCabie final hosE, the larvae hatch
parenchlma,
In che hapatic
tripatic portal ci.rculatlqr.
-!F larvae mlt four Eirrps
females lay eggs in
fertilized
ani Uecoireadults in approximately l-2 r.reeks. The
with
resPonse
they incite a pyogranulsscous
the hepaCic parenchyura-where
of
tire after
eosinophils.- Eggs renrain viable in hepatic-tissue for lggg^periods
be{ore
edryonatidl
che deich of cn6"worms. These eggs ru.ric get into the soil for
they are infecEive. This is acccoplished-in ane of tr,p $tays. .The eliTal_may die
and'subsequentdecornposition of iri body liberates the eggs .and these fall co tne
soil. AnocLrernechanism is wiTenthe host is eaten by a predator. The eggs
digesEed frmr ttre liver of rhe host are passed in Ehe
predator,s feces. sr.rch
PredaEorsdisperse-tbe eggs
and thus r"y'9"-u"rred cransporc hosts. unedryonated
-Finar
;fff; ?5t5-*:i;;rive.
Cqrcributor:
hosrsareinrecr"a
bv-i"s$;i;;;";ilx;#:ss,
Bureauof Biologics, FDA, 8800 Rockvilre pike, Bethesda,
I-rD20205.
References:
r. tsenlrschke,K.
lqghology of LaooraEoryAnirnals,Vol. II,
Springer-Verlag, LglB,"tpp"l:i1670-1671.
2 . G r a h a mG.
, L .: Parasitism in uonkeys. Ann. tiIYAcad.
Sci. 85:
(3 ), 8 4 9 - 8 5 1 ,1 9 6 0 .
i-
case rr - R 27979- (ptrTP L720287).
ontror adurt mare Fischer rac thac died
At necropsy, the rat was fourd to have a urilaierar abdminal
nass.
cqttributorts Diagnosis & cqg*ottlq: Teratoid nephrona.
Ttris turDr is well
d;-;;'co1'rnar
epirhelial
cells, scne of
"ir
t'tttich
bear cilia.
we'have seen 2;th"r;i*ir"i
a.-rs in a series of about 500
rB44 rats. Both of these were smalter rtran-iti".-"-;i.d*"""a
ni"tolqgically ro
be benign. Tbere appears ro be dlt;;-;#-in
rhe lirerarure.
spcttaneolsry'
g.risin
ultqeceEznlne(t' 'mls cliagnosis tras reached
*0"
!yro":
^S1"9,gp"lTgt,,I1rl
afcgr irc
discussion
^L"r-",
oi td'lesiqr.
jfgl":::^::F:::* ;;{r$,. qr;;loilI
papurary
adenocircinon,
Sj53::tr*:"ffi
andmixEd-ia"*"'cinqs.
n;
5i:ifii:{Hi-;"ffiffi
SB:"'r:lk
by manyand irrcluded parane*o"prt"i"
'ttre e*i, fr.rluriin and worrii".,
dtrcr reninanrs,
prostate and seminal iresicles.
exaci'oiigin lras not determined. Arpther
19:i* Seggrte! in the
on someof rhe slides wasa
TeTaining.rgngr tissue"presenr
mild mrltifocar
interstitial
nefrrriti". ctr"..[rerisrics of che EurDrdiscussed
were the larse tubular-ductular'suructures rined-by-;-;ei.-ii
pseraosrrarified
ciliated corLnar epitheliun *a uiJ-iil;
cJ'J"n"" connecriverissue srr@
surrotnding these structures. Large areas of the stroua appeared
my:(@atous.
Nrclei of the epithelial, couponent-vrerg
uasarry locared *i-riio"es
eosirophilic to arytrophilic nateriar-*i--p.esent in Ehe lrrens were rare. A
Pul",
of several
tubules. ScaEteredmit6tii figures were observedwittril ail
rrr*".
fu scrorr,"
blended into the-adjacent r.nai strorna. Tbo germlayers nere
represented
in the
EuDr, not 3- Classification of rhe lesion
t"."toma therefore wouldbe
inappropriate. The tunr is consistent with"3-"
-n" ."ported teratoid nephr@a(l).
Primary renal neoplasmsof the rac are uncourrrrn.
Contributor:
Carnegie-lbllon
Institute
Referencet
!,,r-et aL.:
Vec.EE66lf.-lf6 : TlTayi-E,
130-l3l', Lgig.
of Research and FB,.
SpontaneousteraEoid nephromain a rat.
- 2 -
Case III
zoo.
- N78-577- AFIP L7lL325
rsrkey (Cercopichecus cepirus) iroused ac a
ffied
o@rtebral
, fe.nnle unnkey had a historl
fracture. An infection in the bane of che cail was created 4 mnths prior to signs
of severe diarrhea and seizures. Seizures ccrcinr.redfor one day and the animnl
died.
Besulcs: Culture of granulomatous massesrdere positive
-Iabo:agory
.
I{yc@acEetr'un cuDercurog1s.
for
Contributor's Diqglgsis & Qqryg:rts: l€ningoencephalicis, granulcrnatous, focal,
seve
necr5sis: 6tiology Dlycobacterium
tuberculosis.
-ffi
tGcropsy revealed large caseating lesiqrs in the rediastinlu,
trEsenEery,uterus, cerebrtm, eurdskin at the tail head. These lesions aII
cqrcained acid-fast bacilli.
Ttre liver, intestine and spleen were free of
lesims.
Ulceration of Ehe skin has been reported in Euberculosis in prirnates and
the skin nay be a porEal of entry for the organism. I\.bercles in the cranial
cavity have been reported but are not csur.
litis,
cerebru,
granulmatous, focal, severe,
brain, Cercopithecus cephus.
nci oiogic oia6o6-1ffirEiltoTfr'.
tuberculosis.
EEioloqy: 4l,lycobacterir.m
contributorrs diagnosis and c@rts.
ComrIg:
tgrEwftT-Ehe
ffi5=ffiil6E
Ttre suheningeal and cerebral expanelle. necrotic mes was cqnidered characteristic
of the'disease. Cqlete encapsulatim was noE present in mst sections. the
remaining neuropil wis urrenarkable with the exciptim of a large focus of gitter
cells and loss of parenchymaln s@ sectiqrs.
Tte route of infectiqt was
cqrsidered hematogenousto the reninges with subsequent spread lnto the cerebral
Eissue. Within the center of che granulourathere were foci of mineralizatian and
in tr.fierculous lesians in
nondescript necrotic debris. Mineralization is rncmt
periphery
priuntes.
along
the
of the lesiqt
lnflamratory
cells
nonhr-man
Ttre
consisted-of ly4hocytes, plasma cells, macrophagesand occasiqml mrltinr,nleaced
giant cells.
ltre role of these cells in the pathogenesie of the lesions was
discussed, alcrg with the significance of the caneousnecrotic naterial in tte
center of the lesian. Plasma celle play a sigoifcant part through release of
lynphkines and MIF and aid in cytolysis through acrivaEion of the cqlcnc
cascade. LSiuphocytesplay a role ln the necrosis of tissue Ehrough direct
cytotoxiclty of cells and activate mcrophages and my aid in the formtion of
giant cells.
llacrophages in turn elaborate urokines wtrich enhance the activatiqr
of lphocytes.
Macropbagesalso are respdrsible for Ehe phagocytosis of che
organism. I'tycobacteria can persist and divide within these macrophagesby
preventing fusion with lysos@s.
Lipopolysaccharrides (waxes) in the cell wall
iray prevent Ehis fusitrl.
the urycolic- acid qr the cell surface is -respctsible for
This mycolic acid is not
acid- fast staining characteristic of these organim.
degraded easily by macrophages. It has been reported that the caseous material
coiltains few nycobacteriil Srganisms due to rhe-presence of proteolycic and other
enzyrcs released by inflamatory cells aE the periptrery of tne lesiqr.
Liquefaccive necro-sis on the ocher hand ray contain large nuubers of- proliferatirg
excracellular organisuls in the absence of Cheseenzylltss, and thr.rs enharce the
- 3 -
,
sPlqaq of organisms (3). HosEresponse determines che outcqrp of the disease. An
acid fasc sEain provided dercnstrated intracellular acid-fast organisms with
occasiqtal macrophagesat the periphery of the lesion. primary derebral
tuberculosis in
primates is cbnsidered urcornrcn but his been ieported
?o{*.n
(2). fllberculosis
in the-cranial cavity of hrnans usually involves Etre basal
meningesand occasionally produces tubeicuroa formation (4).
Cqrtributor:
usAFschool of AerospaceI'bdicine, vsp, BrooksAFB,rx 7ilz3j.
References:
T. rar
!. w, ! Htrnt, R. D.: Bacterial Diseases. rn pachologyof
LaboratoryAnlmals,
col. rr, pp_ralg:142r, Benirschke,K., Farner, F. M. & Jcres,
T. C'' Eds., SpringerVerlag,-M, 1978.
Z. Dastur, D. K. et al.: The pathology and pathogenesisof tuberculosis
encephalopathy. Acra Neuropathololica O:"gft-f 2, tg6O.
3. Dannenbug, A. M.: Liquefa-tion of caseous foci in tuberculosis.
Arer. Rev. of Resp. Disease Li3z 257-259, L976.
case lV - 0070 - (AFIP U58837).
_--_-.
e cat. The cat btas-presencedto che referring
veterinarian with the coaPlaint of anorexia, depressiin, ard icterus. Tne anidal
died despite supportive cherapy and was neciopsied.
At rncropsy the carcass was markedly icteric.
q u aHggagory-ResulEs:
n@df1uidwis-presentinthethoracict"uiuy.Liver"."enlarged and had a nrttled appearance.
A small
Contributor's Diapnosis: Feline cytauxzoonosis.
Attendees' Diagnoses & Cqrnnts:
is and herorrhage, acute, diffuse, severe,
-,,-_river. 2) Pneumnia, interstitial,
subacute to chroiic, diffuse, mrt6a to severe,
ly"g. 3) vasculiris, srultifocal, mild ro modeiace,lung ;nd-riv"r.
i) fgragitecda, uurocytic and erychrocytic, rulrifocaf ,:f-t
[rSHcar
(feline).
"na-fiu"t,
_Etiolq3ic Diagnosis: Feline cytauxzoonosis.
Etiologr:
CJtaulzoon sp.
Ccmn9nts:@osEFtistedabovewerereachedaftersmdiscussiqtofthe
lesions Present in theie thto tissr,res. Other preconfererne diagnoses listed -"qir,"lly
by
parcicipants included phelebitis and necrohettnrrhagic hepatitiS i{hich were
acceptable. .Soe attendees reported observing a fEw parisicized red blood Ait;
conEaining rirg forms. A few ieported. tesiqr6 within'srnall arteries in ttre iLrrg
whereasncst observed che lesioni in the chick srd rhin walled veins in the h.ni
and liverTtre cause of the lesiqrs in the liver were not speciiic"ffi
J.c"rriE"a
but felt to be due to anoxic change. Scattered hisriocyric/foirncytic
ioa-rupft"t
cells in che liver Ltere parasitized. Tirere lras sorrequesciqr as Eo whegher
hePatocytesalso were paiasitized. Discussion of rhe'inceisiiiiar
and-viJcutar
lesions in the lung iniluded the diffuse interstitital
thickenins, hyoercrophied
--alveolar lining cells, macrophages
within alveolar spaces-"cmE'of';aigh-'
- 4 -
concained pi$Ent (heuosiderin), vasculitis, and the presence of large num:ers of
organism within circulating cells in vessels. Occasionally it appeired alveolar
Iining cells also were parasitized. Sosreconfusitrl existed to t*retter Ehe cells
Palas.it-ized in- the vessels were rsrocytes and/or endottrelial cells. Hypercophyof
endothelial cells was prodnant in rcbt large vessels. lfultifocal neciosis otvessel walls with hesprrhage into the adjacent parerrch5mawas present in src
sectiqrs. The life cycle of the parasice was discussed and coqared wicb the
disease in wild African ungulates. A fatal disease attributed to this oreanism has
been repotted in a giraffe-in Africa. An ixiod cick is suspected as the Siological
vector of the disease in cats in the United States. The cat see"toan unlikelynatural host of the organisn in that the disease is invariably fatal.
Nr-reroirs
wild sylvatic rodents have been inoculated experirentally with this organism buc
have proven ulsunceptible to the infectiqr (rnp:blished work). The bo6cat may
serve as the reservoir host in this courtry as a carrier state has been observed
(unpublished material) .
Differential
diagnosis includes Eepatpzoan sp. that have been observed in wtrite
blood cells, lung, bdne marror, and fine-r offiatinally
infected cats. Hepatozoan,
lpwever, 9*g not infecc red blood cells and genetogeny occure in neutrophils in
che circulacion.
Schizqrts of this parasite have been observed in the slleen, bone
marrqt, and liver.
Schizonts bf Cvtiuxzoon tr,avebeen reportd in hypertiophied
cells (probably_histiocytes) linifrTliffillls
of blood vissels in miiy orgins (4).
hrozoites are found in erythrocytes.
Cantributor:_ Veterinary Diagnostic Center, Universicy of Nebraska-Lincoln,
Linc6l6,-ffi'85'83.
References:
E.: A fatal cytauxzoonosis-like disease in cars. JAVTIA
168:
ffilft.
5 8 8 - 5 9 6 ,L 9 7 6 .
2. !{ightman, S. R. et al.:
Feline cytauxzoonosis: Clinical features of a newly
described blood parasite disease. Feline Pract., llay 24-26, L977.
3. Ferris, D. H.: A progress report an tbe status of a new disease of AsErican
cats: Cytauxzocrosis. Cory. Iumtn. Microbiol. Infect. Dis. Lz 269-276, L979.
4. Wagner,J. E. et al.:
Experinentally induced cytauxz@nosis-like disease in
doestic cats. Vet. Parasitol. 6: 305-311, 1980
5. lbCutly, R. M. et a1.: Cytauxzoqpsis in a giraffe in Zululand.
OnderstepoortJ. Vet. Res. 37: 7-10, 1970.
TII{JIIIY P. O'NEILL
RegisEry of Veterinary Patholqly
DeparrnBnt of Veterinary PaCholory
- 5 -
Histories
AFIP !,lednesdaySlide Conference No' L4
14 JanuarY l98I
case I - 79-727- (48!-t716400)'
se caE' hesenced becaus" ot 9:g:::t-iTr*^
.
, * . f f iz , *"t ' b u r a r i o n p ro g re ssi n g to co nvu1siqts.Sidesteppigg.- 9Pl.ac1r
B
On necroPsy the-rigfrt francal
respqlses aUs"r,i;r, ieil side, aEpt""""t qt rignt'
The crinlal portion of-the
natErial.
sinus cqrtained g,;kginou", *t*i-""i"i.a
as in sinus' frt cut
traterial
aaE
the
with
h&ispnere
;iltil ;;b;;i
"9ygd aie, r"pfacing normal brain parernhymaof the
sectiqr there was a firo, httriti;;
right cranial cerebral hemisphere.
Case II - 7913-8!-:
P L7578
Otrtbreak of
herd wich several other foalsthis one
except
trear-nFnt
respcrded to antibiotic
*ricb died.
'
case III - M9-420 - (AFIP1757300)
barror iq eood^nutritional conditiqr and
crosr5F6t
mare
ffiyoLrng
ownerut grouP-of-pigs m a
was presented ior exoinatid.
'iur"p.r"l;-;i
r*igfiiffipo*ds
g.;und wtrole oats for a Erea@nt of ulcers ' He
rarion wirh 500
Ttre Eatio w€rs
lcnered rhe oats ;;500 lbs per"i* -q-pigs started to do-poorly'
I'atglv' 9!i P-ig: :::"..1:O t*
16%crude protein-nade witlr iott-*aJaZ'sQprmtt.
ttrat looked very Sood: Despite Ehis, pigs weregrohtlng
corn frcr ordner;t
""op
iespiratory probfeos. SoE died.
Jf"Jfi-*a-devefoped
Case IV - W-268Uf8686 - (AnP 1764666)'
and poT!ffiia.
'
!!L - Li-&^-,, ^€ -'r.,
le Llelsh Corgi with a history of polyuria
T\romicroslides'
Laboratory Results:
(See next page) '
TII.OIHY P. O.IiEILL
Captain, USAF',BSC(VC)
neeistrv of Veterirrary Pathologl
of VeEerirrary Pathology
Uefrruint
AFrpwednesday
rft?"Jit;ference - No. L4
l4 January l98l
CaseI - 79-727- (AFrp Uf6400).
- - - ' ^
nese cac. Presencedbecause
-iio*-rc.ppi?g
of depression and
anorexla ot z weeksduration orogressing Eo convulsions.
and placing
cesPonsesabsent or.rrgft side, dEpressef,* rijr,c.
0n
necropsy
Ehe
rignt
froncal
sinus
containedgelacinous,cirrei
porrionof rhe
righc cerebralhenisphere
"oioi"o-*i8li"r.*'Til-;;fiiar
coveredriil-il;
;]il'rnacerial
as
in
sinus.
0n cuc
section there was a firm,
whicish
righE cranial cerebral hemisphere.
"r""
Laboracorv n$$$.
cre @
rupril;g
normal brain parenchpra of the
Impression snEars aE necropsy shovued
a preponderanceof
sein in cn" n*pt";;;;
H6Esecrions.
ConEributor's Diagnosis: Ependyrmra.
AEtendees'Di
is & Couments:
i'rJEPf,ioroglclJlagnosis: Primitfve neuroectodermal
neoplasns wich some
brain, siamese,
feline.
"p.nlffL9tfj::":!i*i:1,-:iglrc
cmrenrs: The.majoriry-ofEnepr""o"i.r"*"
""t"u""r-rr&ispnere,
hi"gn.r;;;;fi,1;il;";;"ffi:i"
ependymraor ependyunolastqna
ts d. pii*ry-ai"gnoEis.
oiicerenriar diagnoses
subudEEed
included'adenocarcinoma
of cfie
-Hisrol;ti;;1-f;rur""-oi-ct"
.piiir"ri,-]-neurourasr@ra,
meEastaticadenocarcinon,and pinealcyrora.
"ir,fi"ia"i
flpr"",
coruented m were Ehe invasive nature, compression, iSrmation
of n,,*rous rosegEes,
neoprastic cells tining up il rolirs, lack o'i norryr ependynal
lined
vencricle, .nd
mulEifocal necrosig plegent in the't,rrci.
c"iiurar cnaracrerisrics included che
tarl coluurar epicheiial rosetces forrea
small lrrrens, basally locaLed
nucrel, the Presence of cilia ac the apice"ro*J
of the cells, and'high
- . . ^ 1
- !
- l
milocic race in
several areas of the EtnDr. There $ras an
- apparent lack or seciLtory product in
boch the lt-uens and within cell bodi;.
Md;;;seEEes
rorphologicarly
resembte
those described
in ependyrnnas
of manr"ro=J'rL;;;;;
;;5il;t
which arso are
described in rerinobias6mas. e9e.u{or;;;;";
presenE,
form around
small blood vessels and strota within ttre trsrcr."r,i"t,
This"G;-;;;
neoplasmis rpre ccnplex
chan-ependynrmaq
or ependyrnblasLmas. An olfactory neuroblascoura
corpletely ruled out basedon Lhe clinical tisiory,'iroweveil-i*.ct" courd noc be
not characteriscic for this tutrDr. other differenuials which rnust formaciar is
turDrs of che choroid plexus and astroblastomas. N{"1{#i-"p*,ow"nrsbe included are
are
considered rare in douristic animals and *y *t"rtasizE via eie
cerebrospinal fluid.
Contributor: VeterinarV DiagnosEicLaboracory, KansasState University,
I'{anhFtEilTfT-6506.
Refererrce:
RilCTfE"fi',
L. J.: Tunrrs of the cNS, 2nd series, Fascicle 6, Aclas oi it-mr
Pathology, AFIP, 197'2,pp 104-126.
Case II - 7913-80- (AFIP L757255).
nerd wiEh several oLner foals. Outbrear of
diarr-hea in foals and all foals respondedEo antibiotic Erearrpnt except this qre
wtrich died.
Cqrcributor's Diagnosis & Ccrents: Granulmatous colitis, diffuse, severe
hepat
Couents: Ttre-Grm positive organisms in the foci of hepatocellular necrosis
are cqrsistent with the diagnosis of Corynebacteriuu equi.
AttendeesrDi
ls&
s, granulmatous, ulcerative, diffuse, severe,
L, equine. 2) Necrosie, acute, nrltifocal, rcderate, liver.
Etiologic Diagnosis: Bacterial colitis and hepatic necrosig.
Etiology: -Corynebacteriuureoui.
uqmlents: Other mrphologic dtagnoses suhitted prior to Ehe confererne
included necrohorrh.agic colitis/enteritis
and recrolizing hepatitis.
There was
sos:Equestign- as to wbettrer ttre tissue represented Large oi smitt intestine.
This
was resolved by discussion of the histol-ogical appearanceof the tissue and
disclosure of the contributor'g observations. Fiitures of the colanic lesians
included diffuse severe congestion of vessels in all layers, diffuse markededena
of the suhrrucosa,diffuse infiltratiar
of the lnrcosa with histiocytic cells witb
mrltifoca| Branuloa formation, €rrd mrltifocal ulceration of the rucosa present in
A few attendees reported observing throdi in small mrcoial vessels
ryany qectiqrs.
in additim to the changes reported above. gacteiial orgcrisns were observed in
Ehe l,anina propria, usually associated witlr the granulmae, and within the luen of
the olgan. The lesiqrs in Ebe liver llere cpatible
with foci of acute necrosis.
The disttibutiqr
of tfre leslons was considereil randm. Bacterial organisre were
observed within hepatocytes at the periphery of these necrotic foci.- The najority
of the participants suggesred Satqqqrella sp. as the probable etiologic agent
responsible foi rhe leiiqrs.
OrE'eTTa-eEieffi'discuss;d differenri"liy
irfrtudea:
piliforuis.
sp.,
E.
coli,
and
Bacillus
However,a Gra stain tlren was
lh:lgglIa
projed-ef vf ich-ffi'rrare@
coccobacilli both wirtrin rhe
colonic and hepatic lesiars.
Based trr che rcrphologl ard staining characteristics
of the organisrrs it was concluded that the organiqrrg were coqatible with ctrose of
Corynebacterirm. -C.# gqui has been reported prirnarily as a pathogen of ctre
resPiracory tracE of horses. Recently lesicrs attribuced to this organism have
been described
in the intestlnal
Eract of horses (l).
this organism in horses are seropurulent arthritis
Other lesicls
atEributed
and hepatic abscesses (2).
to
Contributor:
Ibparuent of VeEerinary pathology, College of Veterinary
-Iln];inFsiEy
rcdiETne,
of Missor.rri, Coh-dia, I'{l 65211.
Reference:
fruner,
D. W. and Gillespie, J. H.: llagan's Infectious Diseasesof Drescic
Animals, 6th Ed., Cornell UniversiEy Press, L973, pp 321-323.
equi enteritis in
2. Ciuprich, R. E. and Rooney,J. R.: Cory@
*
f o a l s . V e t . Pa th o l . t4 : 9 5 -1 0 2 ,'L977.
Case III
- N79-420- (AFIP U57300).
barrour in good nutritional condition and
weigEiifTlfpoyn$g was preeenEedfor exsrinatiot.
Orsnerput Ehe group of pigs
-Heon a
raEion with 500 lbs pet tor of gtourd ntrole oats for a cr6atnsrt of uicers.
lor*ered the oats to 300 lbs per tcr and pigs started to do poorly. The ratio was
16%crude protein madewith iorn and 38%'s[ppfsrnnt. Iacel], rhe pigs were fed new
corn frm orfiier's cqop that looked very good: Despite chis, pigs
I L were groring
slorly and developed iespiratory probf;n6. S@ died
Fibrinogen 700 qg/dl.
1+ trlctnis
suis-ova and a few large coccidia oocysts on
5 seeds flIEldf-EiStolaria
in feed anA t seed per to. of
Laboratory Resulte:
fecal floatatiqr.
Sassid obtusiofolia.
Cmtributor's
Diapnosis & Cmrts:
l, chrmic-active, mrltifocaf and
coalescirg, severe with mrltifocal adenmator.rschange of alveoli.
Ccrrmnt: Lesiqrs present are cqrsistent with pyrrolizidine alkaloid toxicosis.
Attendees' Diagnosis & Cments:
,incelstitial,pr olifer ative,chr qtic- activ e,
diffuse, severe, lung, crossbred barrovl,' pib.
Etiologic Diagnosis: Toxic preumritis.
Etlology: Pyrrolizidine alkaloids
Cmtts:
The uotphoLogic diagnosis and etiology were reached after
considetable discussion of the lesims and disclosure of the laboratory findings.
A wide variety of prgcgnference noqphologic. diagrnses and suepectd etiologic
agents were suhitted by the attendees. The majority
favored a
-while of the psrticip4rts
proliferatlve
and euppurative bronchopner-unnia,
others ireferrid a
chrqric-active brqrchoEreumria with adenmatous change. The etiologic diagnosis
msc often listed by the participants has enzootic pner-mnia of pigs. Ochei
etlologlc diagnoses suggested by attendees were foreign body pnetmria, verminous
pner^mnia, €rrd ccrbined bacterial and rycopla.arnnpnermria.
Histopathologic
features included diffuse prminant hlpertrophied/hyperplastic
alveolar linirg
cells, diffuse interstitial
fibrosis and infileraticr
wittr mixed inflrnratoel
cells, mrltifocal presence of neutrophils within alveoli srd brmchioles,
rultifocal
erpbyscnratousctrange, Lhe formation of large nLders of giant cells and
che presence of lyuphoid follicles adjacent to brqphioles.
The nixed inflamatory
infiltrate
within ttre interstitiLa consisced of lyqbocytes, plasma cells,
macrophages,neutrophils, ard eosinophils along with the proliferating fibrous
to
cannective tissue. Ttre rcst striking feature of the lesiqr was the mltifocal
diffuse adenoatous change within the alveoli.
It was pointed out that this is a
nonspecific change in lr,nngsand can be prodrced by a variety of agencs (4). The
origin of thd mrltinucleated giant cells ras discussed, b,rt renained r.nresolved.
Several attendees felt the giant cells resesbled foreign body giant cells and
S@ qtrestict
others felt many of these cells reseobled syncytial giant cells.
tlhether
an
interconcurrent
remained in Ehe minds of many of the parEicipants as to
rdas
lyryhoid
peribrqnhiolar
presenc in this pig. The
bacEerial or viral infection
could
dtange
follicles
were suggescive of mycoplasmainfectiqr and ttre adermators
Ehe
to
to infection wieh an influenza virus. Ttre discussiotl turned
be artributd
and
prevent
mitosis
acciqrs of pyrrolizidine alkaloids. Thris Eoxin is kncxJnEo
thus nay actount for che hypertrophied alveolar lining cells in this case (I).
Hepacic lesiqrs colsisqing of fibrosis, bile duct.proliferacion,
negahepatocytes,
and infiltration
of inflfutory
cells'r.o-a-Fo"i'of
delen.r"'iine
and necroric
hepatocvtes anc fibrous conneciiv;-tG;;-;;
;h;-"ii""-i3ii=ilii*"
of tbis otanc
(3):.. The lesiqrs present in rtre-rurg"i"-;h;Îl"'"'[iu"
ryi"Tit'g
been
:
descriuea
and
are cmparible wirh rhis'roxicosis (I,2t.
cmtriburor:. Divlsion of cqarat.i.ve pathorqgy:_go*
V e t e i @i n e , U n i ve ru i ti .o fF 1 o ri d a ,GainesviIIe,FL326r 0. J-rlI,
colrege of
References:
T.EraIng;
J._D. J. et al.:
Experiuenral poisoning by Senecio jacobaea in
pigs: Path. Vet. Lz 204-220, Lg64:
2' Huxtable, R. J.: Ppiolizidine
alkaloids and the ltrng endorhelitu: A
paraliq1 o-f
lurs dpaee reiuttirE i;; ;iili;;i,,s
Eoxins.
3' Keerer, R. F. 6t al.: gf%cil-;i i;i";;uE
pknrs on Livesrock. Acadsnic
Press, ryetryork, 1978, pp 16f-197.
4' har' A' R.: the characteristic
-i: cel1s of the lrng and their reaction to
injury. parr
r. vet. Butl. sai 16.
tll:rr45l tury Ls&.
Iv - 80-26871L8686
!{elsh Corgi wittr a history of potyuria
Iaboratory Reeults: (Seenext page).
cqttributor,{Pgtr@
Adrenar corrical carcinma, with necrogis
"YLYrs
ana
crents:
Basedar the clinicar. gigp" and-raboralotr findings, the
practitiqEr treated the dos f"T-gTlt3['"
ttorc,,E with o,p' - DIx). Approximarery
ane utth later.the-dog wae-lilled at tbe mier's requesr.
select ciseuesr€re
suhitted for histolggl" evah.ntion io-in"rin.-*
*irq!"a
adrenal, rhe lefr
adrenal, liver, ard ri-Tg wtrich n"a
"igbr
nodulEs
of
ilarying
sizes. rhe
pituitary ltas rpt exaiied. Ttre dlagnooi"
",rerJ*iiitoicortical
carcinoir
was
onf irred bv the.presenceof ntrerous rerastatic"a"enal
;irhi;Ae
rurgs. o6her
findinge inaluded'oc."si"-"f--*r. zqra fascictrLa foci
ard reticularis in che lefc
adrenal and severe fatty
Adrenal cortical
infiltration
clers
of ttre-riLr
with hepacocellulas danrage.
are an uncourn
caule of canine hyperdrenocorticism.
rhe rcsr cqnur causeis bnarerg coiiicai hyp.;pG--:'fiit$
idi"Frhi;;-
resultine frm a
pituitary adenoa. ciinicar signs--a1d-GE;;;;tf*::iq"r
findings-are due to
the lor€';;;
excessiveglr.rcocorEicoids.
"rt..i"-"e
Ateendeest Di
diffuee,
adrenal.
l) Carcincna, corEical, adrenal, wich
crosis and mirpralizatiqr, mrlcifocal co
cmrte:
Ttre mjority of Ehe conference attendees listed the diagrroses
prher preconfErengimrphotogii-aiigrps& risred r*re aderpcarcirma given
with
trEtastasis
a!ve,
to che lmg and carcinoa
or ena&rine origin retaetatic
to ehe lug.
HEMATOLOGY
419/8o
s/3180
5/L2/80
RBC (x106/p1)
6.44
15.6
':"0
5.26
L2.7
35,700
91
5
2
0
I
0
5.03
13.2
3 3, 9 5 5
94
I
4
0
0
I
nb (t/dr)
wBcI /pL)
Seg. ueutrophlle (Z)
Noneeg. neutrophila (Z)
Lyophocytee (Z)
Monocyto (U)
Eoslnophlfe (I)
Baeophlls (Z)
SERI'u
:
4lglao
slL2lso
106
L2
.5
413
735
51
6.0
3.2
155
4.1
117
3
.6
730
840
107
31
5.3
2.5
153
2.3
URTNALISTS
4/9/80
slLz/80
Color
ptt
Speclflc gravlty
Protel.n
Ketones
Glucose
Blle plgnenrs
Blood
CIear
6.0
1.006
3O+
neg.
neg'
neg.
neg.
Clear
PIASI.{ACORTISOI(ne/nl)
4128180
Faetlng glucoae (ug/af)
BUN ( ns/dl)
Creatlnlnc ( ug/dl)
Cholesterol (ngldX)
Alkallns phoephataac (nU/nl)
SGPr/ALT (uu/nl)
scoT/AST (nu/nl)
.Toral proreln (gn/dl)
Alburntn (gu/dt)
N?+ (urEq/l)
lf (nEq/l)
pre-ACtI
2 hr. post-ACtn
s90
80
290
6.0
1.005
trace
neg.
neg.
neg.
neg.
Hyperplasia of the adrenal corcex also was uentioned by-a few. Considerable
cencered arornd the clinical laboraEory results given by tbe
diicuisim
Ttris tncluded changes in the hemacogro guch as the nild to rcderate
cqrtribucor.
neutrophilla, abeolute lyqhopenia and eosinopenia. Serumch€aistry abnormalities
irrcludid increased fastiirg'glucose, elevaEed 6err-mcAolesterol, mackedly elevate-t
serLm alkaline phoephatase m both occasicts, marked SGPI elevatiqr ini-tially-and
later a mild inireaie, Iqr total protein and albr.min qr the secctd saple, and -1or
Abnosrat findinge in che urinalysis included
serL6 potassiLm on the last sapli.
Ttrie apparent Ploteinuria
lqr spAciflc gravity and hiih urinary procein excretlqr.
led siEB?atteidees to euspe-t a ccrcurient renal digease in thie casg. - -The plasma
cortisol levels nere felt-to be exaggeEatedbefore A6III c,hallerrge and diaglostic of
hyperpLasla/neoplasia after AGIITchallgnge.- Histologic features_gf chi primry and
nitastarfc lest;ns included the endocrinoid appearanceof the cellg and the
s@
preserre of neoplastlc cells withln veseels alctg the capeuf" qt ltte-g$d-in
@ntiqrcd obeerving tLmr_cells within foci of
iectiqrs.
S@-participmts
mineralization a;d necrilele in the adrenal. Ttre specific cauge of the recrosis ard
in the adrenal may be attributed to the tulDr iteelf or to the
mineralizatlm
Ereaffiit rtrich was given to the- aninal. Ttre persisteCIce of the zcra glreru_losa
present in nany sectlms indicated therapy as Lhe possible cau8e, as 9,P: - FD i"
Lnq{n to sparsthis layer of ttre cortex.- Correlati.qr beEweengerLm ctreoiscries ard
Ehe neoplain wae dfucGsed with specific reference to the elevated liver en4[tEs
and their parhogenesis. Ttre elevbted SGPTcould be attributed t9 hepatocellular
wttich is cmnly
degeneraEtin and necrosis subeequent to fatty retmrphoeis
phosPhatase-Ievels are
alkaline
serun
Elevated
de6cribed ln hyperadrenocorticls.
phoephatase
via
coqression of the
productim
of
alkaline
attribtrted to iircreased
e)€cE
11rrer,_hotever,.!Fe
in the
bile dr.rctgcaused by the fatty retmrphosis
(2).
kncrdn
reasons for tbis intreased SnF prodrct-iqr are rmt cqletely
-Ttte
trypercholeeteroleola could be attributed to the hyperadren@orticisn and/or renal
disease.
Cancributor: Ibparmrt
IE
of Re=ffChTas-hingtur,
of Coryarative Pathologl, Walter Reed Arury Institute
20012.
Refererrces:
C.: Turcrs of tlre endocrine glard. In Tumreîn Deetic
Animals, 2nd'ed., University of California Prees, BerkE[ey, ]978.
Z. Con"n, S. J. et al.:- Hyperadrenocortici* ig a-Qg^qith_adrenal and
pituicary
J. Arer.-Anin. Hosp. Assoc. L6z 259-262' 1980.
nJrpbeia.
'
the synthltic A6Ilt scimrlacicl test and lleaauresEnc
et al.:
E.'C.
3. fifAair,
A6tll levels: UeLful diagnostic indicators for adrenal disease
of endogenors if".r"
ln dogs. JAAIIAL4: 52tr53L, L978.
+l tet5er, J. C.: Canine hyperadrenocorticisn. In Cr-rrent Veterinary lterapy
VlI, '5.W. B.-saurders Co., Philadelphia, 1980.
Onens, J. M. et a1.: Hypeiadrmcorcicisn in tL* dqgt Canine Cushlog'P.
Syndrre. Virc. Cfinice of N. iierica 7: No. 3, W. B. Sauders Co., Philadelphia'
L977.
P. O'NEILL
TIIOTTIY
BSC(VC)
Capcain,USAF',
PathologY
VeterinarY
Regiscry of
Oeparqr"nt of Veterinary Pathologl
Histories
AFLPWednesdayS1ide Conference - lilo. 1"5
2l January f981
CaseI - 80-68- (AFIPt758395).
cir-oici mle re<i kaqgaroo(!&gaieia rufa) ririch Haa
leg.
e3ecE6ifffi its potrh *ren the rcther was frightered by dogT6il5rffiher
joey,
but it died several urchs later.
ln attempt was maileEo hand rear the
Caee II - 80-10933- (enP I75839I).
Three bulls died in a 3-{ay
4-year-old bull.
periocl-fi-a pasture wittr-2 ottrer bulls nd25 cots. AIl aninrels were fornd dead
without previous signs of illness being obsetved.
enlargsrryrt and redde_nirg of
Laboratory Results: !,larked splenregaly,
were fornd at necroPsy.
horrhages
diss".ttinated
serosal
nooes and
PrescaPul-ar
Case III
- AF'IP
dog occasicrally
passed blood clots
fernale Saint Bernard wich mild
a Ii-urth-old
The
void urine and rcre frequent urinatiqt.
(2-neek dtnatim).
2+ for blood, 2-3 Wff.|LPF
Laboratorv Resulcs: Urinalysis:
Culture negative
Radiography shored a mass at the bladder neck ct PneLmcysEography.
CaseIV - ffi-2L4 - (AnP 1761490).
rst pregnancy. Alorciqr at 9 mnths gestatiur.
tlareEf-EFn
vaecinaEed three linps
during pregnancy.
TIT€I}IY P. O'NEILL
Gaptain, USAF, BSC(\JC)
Registry of Veterinary Pathologl
of Veterinary Pachologl
Deparent
IiEUATOLOGT
4/9180
5 1 3/ 8 0
5lLz/80
RBc (xlo6/lr1)
Eb (e/d1)
6.44
15.6
10,134
5.26
L2.7
35,700
91
6
2
0
1
0
5.C3
1 3. 2
3 3, 8 5 5
94
1
4
0
0
I
r.gc I
frr)
(Z)
Seg. aeutropblls
Nonseg. acutrophlla (Z)
Lyophocytes (Z)
ltoaocytee (Z)
Eosloophlls (Z)
Basophlls (Z)
sERttu
:
4|el80
lastlng glucoee (ng/dl)
3u!i ( ug/dl)
Greatlnlne ( ug/dl)
Cholesterol (ng/dI)
*Ikallne phosphatase (nU/url)
SGPT/ALT(uU/al)
106
12
.5
590
413
fotal protel.n (gn/dl)
'.lbunln (go/df)
l;a+ (rnEq/l)
51
6.0
3.2
r 55
4.t
scoT/AsT(nU/ul)
;:r (are/r)
.-
t3)
3 lJ.2/80
117
3
.6
730
840
:07
31
5.3
2.5
:)J
2.3
! /e/8o
- </ 1 : / 8 0
Color
pH
Speclfic gravlty
Proteln
iietones
Glucose
3i1e pigoents
31ood
Clear
6.0
1.006
3C}+
neg.
neg.
neg.
neg.
Crear
6.0
PIASI|A CORTISOL(ng/ol)
4 /28/80
N,INALYSIS
pre-ACTII
2 hr. post-ACTB
80
290
I .0c5
:tace
;-eB.
reg.
neg.
neg.
Resulcs
AFIP Wednesday
Slide Conference- No. l_5
2l January l98l
- 80-68 - (AFrP u58395
ffirth-old
, male
(|&gareia fufa)
utare red
re(l kangaroo
Kargaroo
(yegarela
wLrichwas
rq{e) hrht'cn
eJ cEed frwr its pouch when rhe urrther was frighEened by do[ilIidEoE-her
leg.
Case
An acEempt was made Eo hand rear the joey, but-it
died seveial rrcnths lacer.
Cqrtributor's Diagnosis & Cunegts: I'fycotic gastritis, cmpatible with
candffiCsuEnts: Heavy yeasts, determined co be Candida albicans, were presenc in che
fecesof.thejoeyforsevera1weekspriortorea_E6-.rffiiwastreatedwith
Elycostatin but apparently
The joey also had rcderate
-it was noE-effective.
and lyrph node atrophy aqq
-sDderate
_broqrclgpneuupnia,-butno eviaence-oi-;t-'--"pi*i"
dissemination of the candidiasis. The
difficulty oi'orpr,a"-r".iing *a .uU6"q,*oc
candidiasis has recently been reported in grey kangaroos.
. !) cascricis, ulcerative, acute to subacute,
2) Lryphgdeqitiq, subacuEe,diffilse, rDderace, fynpi, node.
Etiologic Diagnosis: lvfcotic gastritis.
EEiologl: Candidaalbicans.
ConEnts: GEer eqmTl/"Eceptable preconference unrphologic diagnoses
submicted $rere necropufulent gasciicis, necrotizing g"rtr"itiJ,"or
mycotic
gasEritis.
A few aEtendeeschoughqthe tissue repielenced esophagur
"iorffry riUiir"a
diagnosis of esophagiEis, but rhe cbnrriburor wab piesenr
"ni and
?,*:*gfo,gic
ldencltiecl the tissue as nonglandular stonrachof the kangaroo. Lymphadenitiswas
submitted olff bf a
few.of rhe par-ticipants as rhis tissle was nor presenEin all
secEions. CharacterisEics
of tfre lesion included the erosion and o,irftifo""i
ulceraLion of the rucosa, the large numbersof fungal o.g"i;*,
Ehe cellular
respo'nseof the hosE, and che abrupt ending of the-lesioil at che jtrnc6ion of the
glandular and nonglandular scomach. l'ungi"were observed extendin[ to the basai'
epithefirn and occasionalty inco the submrcosa.
-ulceratiirn
.!Yers-of che_stratified squaunus
---l"licroabscess formation and
the mrcosa were bhserved Uy aif-.
.along
Characteristics
-of che lungi include the m6ssesof fungal hyphae and nirnerous
blastospores. -The,
septate hyphal
forrs were uriform iil diann!rcr and irad-pai"ff"f
-Blascospores
walls and rqe.ular-branch_ing.
$reresrnll, rolrnd co oval, and';fc;;osrDnstrate<l budding. The cellular response $rasmixed wichin the subrnrcosaard
wicbin Ehe lamina propria and consisted-of lyurphocycesand neutrophils. An aLrcst
resPonseoccurred aloqnd fungal organisms in rhi ulrcosa. Changes
?:t:,1.Y:.9Ph111"
node
included necrosis of the germinal denters, deplerion of fyqhoid
in-!!e-Iyttph
follicles, aod diffuse infilcration of the iode by rnderate'nirriers of pMN'i.' tr,e
{PPearanceof the node was described by a few as ieaccive. Tt:ere \,,/assorrE
discussion of the pathogenesisof chis- lesion and sevecal, possibly dependenc,
factors were proposed. These included a changein bacreriri flora-of Lfre
alirrEntar_ytract due to antibiolic a&riniscracion, stress and irminosulpression,
scasis of the stornach. Local facrors such:rs IgA produccionand locai'io*otiiy'o,ry
account for the cessation of the lesions at the jr.mction r:f the glandular and '
nonglandular stosnch. It was poinEed ouE thaE the cor.r's milk formula given Ehese
orphaned animals which is dissimilar to thaE of kangaroo milk in galaccose concenE
rnay aid in the production of this disease as Kangaroos lack enzymes co digesc
galactose.
Caution was expressed that on gross examination of the stmrach these
fungal mycelia may be mistaken for milk curd.
National
Contributor:
Zoological
Park, l,lashingLon, DC 20008.
Reference:
@.L.:Candidiasisinyoun8hand-rearedkangaroos.J.wi1d1ifeDis.
1 6 : 1 3 5 - 1 4 0 ,1 9 8 0 .
Case II - 80-10933 -
P 1758391).
a 4-yea*old bull.
per
Tnree bulls died in a 3-day
b-pasture with 2 other bulls and 25 covrs. AII animals were fourd dead
without previous signs of illness being observed.
Laborator
PrescaPular
Results: l,larked splenmgaly, enlargeuent and reddening of
and disseurinated serosal hernrrhages were found at necropsy.
Concributor' gji@g.ig
frarJ5frl-ssues.
: Septicemia, anthrax.
Bacillus
anthracis
was isolated
Attendeesr D
, diffuse, severe,
bovine. 2)
3) Pleuricis, fibrinohemcrr
Etiologic Diagnosis: Bacterial pner-unniaand splenitis.
Anthrax.
Etiology: Bacteria corpatible wich Bacillus anthracis.
Ccnrnnts: Other diagnoses given by ffis
were pneumnitis and
pleuritis, lynphoid necrosis of the spleen, and congestion and heuprrhage of che
lung and spleen. Scne participants described a capsuliEis associated with the
spleen and vasculitis was listed by one. CharacterisEics of the lesions included
pleural thickening and herprrhage; fibrinous material within alveoli and adnering
to pleural surfaces; and edema,congestion and heuprrhage in alveolar sepEaeand
spaces. A mild inflauuratory response of small nrmbers of neutrophils accmpanied
these changes in the lung. lesiqrs in tne spleen consisted of foci of necrosis,
depletion and herprrhage in the corpuscles and diffuse congesEicr/hemrrhage in che
red pulp. llrderate nulrbers of PIW'S accorpanied these changes in the spleen.
Nwerous bacterial organisms observed in the luqgs and spleen were characEerized as
long bacilli with square ends lined up in a "boxcar" fashion. A bacrerial sEain
deflslstrated Eheseorganisms to be GramposiEive. The clinical signs and
pathogenesisof the disease was discussed. It was felc that death ifnich is al-mst
always peracute in the bovine is attributed to a shock-like condition. The
production of Ehe shock is probably uediaced by the release of a toxin nnst
probably an exotoxin v*rich produces edema,hernrrnage and necrosis. Three toxic
factors have been idenEified in anthrax: a leEhal factor, an edemafactor, and a
protective antigen (2). Another possibiliEy nay be neurocoxin production by che
bacEeria. Other features of the bacteria discussed included the aerobic vegeEaEive
phase which produces the toxins and rhe necessity of exposure to ahrospheric air or
- 2 -
oxyggn for- sn9r9 producEion. Spores persisc for decades in the
environrenc and
remaininfective-
opening.gtni"pryy of carc".r"" in whichanrhiai-i-"lii"p"di.a
is strictry-al"!9$-ap9a (irregal in sqrn cortrrrries)uecaJJe
oFsporurarionrhar
results'
Although the vegetaEive baciui il ilternal organs of r^rropened
carcasses
are destroyed in-2-3 days-by putrefacti*,-td-ilood
exuEJ-fior orifices is loaded
with bacilli rhat sporulate.
Contributor:
Departmentof Veterinary Science, South Dakota State University,
nrootrrnffi-TtOO7.
References:
TIIE66--fKe:nnedy: Pathology of DoBstic Animals, Vol. r, Academicpress,
Lglo,
p. 374.
^ ^2'lp!9itt",
9r L.:- Pathologic Basis of Diseaae- rnfecrior.s Disease. sawrders
& Co., Philadelphia,
1980, p. +7a.
CaseIII - sIP #2 -_(aEE 1667519).
a tJ-trpnEn-oro
re0Fle
lialnt Eernard with nild
slgns of cystitis, prolonged-tlnreto void urine and rcre trequent
urinatiqr. it"
dog occasioully paised urooa clors 1z-wee[-Juraii*1.-.
- G -
Laboratorv
Resulrs:
Hllllryrj:;".?;f"r
blood,z_3:wrc/Lw
Radiographyshcnreda lurss at the bradder neck on pneurccystography.
contributor's Diapnosis & cosnepls: Enibryonalbocryoid rhabdmyosarcorm.
uIpE
a8 grapelike
ch.rsters arourd
the neck of the bladiler and dorn the urechrr.' PresenEeo
il-JA;;""ii[!r
because of inability
to firnctiqr normally after surgery and no further
"uttanacized
evidences of
the cL@r rrere fotrnil in the cadaver.
Attendees'
RhaMmyosarcma, urinary bladder, Saint
Berna-itf,G:Iild. @
CsnFnts: Rhabdoryosarcoma$ras the preferred diagrnsis by all the participants
and several includ{ disgrlptive terms slch as uorriola,-p"ry'p"ia,
and e4,ridr.
Differential
diagnoses given by se were teioyosaicma,
ani'mlxmtous
fibrosarcma.
Featureslr *re neoprasmai".r#a
porypoid and
*r" .t*-iiigi;ous
papilliferous bransitional tined.piojecricrs,
rh" obviotrs-siitdi;;fi^ffific
i"
both dense and ryxooatou{r (loose)'.ri"ng*,i,-f;;ii;-";'i;;rracing-ffiJies'or
Ehesespindle.g"Lt,
rlre preienceoF n iiiroc"l
curg""ti*-*a-h;ir;h€;-
.
associated
with the -and
tL@r in Ltre bladder wall. se atEendees reportd observing
ulceratiqr and necrosis of the urcosa Uy neopLstic invasisr.
A f"lf aEtendees
qepgrled observing mrsruclear inftsmnelory tells associated wich these foci.
of the neoplasticcelti discussJ ller;-rh; pi.*"pt i*
tt,.
!:ll"$'$reftoey
the
rare
aPpearance
"r
of
nucleoli,
the
variatiqr
in
iitotic
rare
::tl,|qleI'
(u-+/nPt) hnEhin
clitferent areas of Che EurDr, Etrceosinophilic fibrillar
cytoplasm
scge of wtrich cmtained cross sLriatiqr or so-called "strlp
p".i,iio.
""ff";-*a
- 3 -
vacuoles present in sornecells.
The discussion tnen curned co cne possiote cissue
cell of oiigi.n for this tuurlr. It was noted chac a large nwrber of'chese tutrDrs
occur aE Ehe neck of tire bladder in dogs. |drltipocential resoderrnl cells of
Wolfian duct rennants in the fernale and Mullarian duct reurranLs in Ehe rele r.rere
speculaEed as the tissue of origin.
There is an apparent oreed and sex
predilection for this Eunnr; it occurs upst often in Saint Bernards and rpre ofLen
in females. Metascasis is rare and has been associated wicn mulciple sur6eries.
In humans the tr.mrr occurs rnost conncnly in males less Ehan L-L/2 years of age. In
dogs this EurDr npst often is reporced in animals less cnan 2 years ot age.
Anocher feature pointed out was Ehe apparent predilection
for cne urinary bladcjer.
The reason the eurbroyonic rennants proliferate
may be associated r.rith cransitional
epitheliun of che bladder as urothelir.rm stimulaEes fibrous conneccive Eissue
proliferation
r,*ren transplanted to sites of bone fracture.
The associaCion irith
hypertrophic osEeoarthropathy and Ehis tmor was discussed. The specific cause is
noE knomr but may be associaEed with neurogenic pachways as chis seems Eo be Ene
case with the thoracic pulnnnary space occupying lesions via che vagal nerve (I).
Direct sisulation via a factor produced by the cransitional epithelium of cne
bladder also was speculated as a possible cause.
Cartributor:_
Star6@,
Deparcment of Veterinary
puilman, WA 99L63
l"licrobiology
and Patnology, Wasniqgcon
References:
f:-TElTfidell,
W. H. and Ackerrnan, N.: Bocryoid rhabdomyosarcomaof cne urinary
a4!_hypertrophic
osteoarthroparhy in a young dog. JAWIA 165:
lFaigl
9rr-98, L974.
2. Roszel, J. F.: Cytology of urine from dogs wiuh bocryoid sarcomaof Ehe
bladder. Acta Cytol. L6z 443-446, L972.
3. Stanps, P. et al.:
Bocryoid rhaMorryosarcoma
of che urinary bladder of
dog. JAVI{A153: f064-f068, 1968.
Case IV - 8O-ZL4- (AFIP L76L490>.
rst preBnancy. AborEionaE 9 rDncns gesEation.
t'lareEl-5-n
vaccinated three tires auiin[ pregnancy.
Contributor's Dlagnosis & Conments: AborEion, equine viral rhinopner.unnitis.
er, lung and'adrenalwith areas of
mineralization. InEranuclear eosinophilic inclusion bodies are presenE,
Particularly in bronchial epitheliun. Eq,rine herpes virus isolated from tissues.
Attendeesl Diagnoses& Conuents:
ia, interstitial, acuce co subacuEe,wiEn
uultifocal necrosis, and nuurerousintranuclear eosinophilic inclusion bodies, l*g,
foal, equine. 2) Hepatitis, necrorizing, acuce, rultifocal, nrcderate,Iiver.
3) Necrosis and henorrhage, with mineralizaEion, mtltifocal, severe, adrenal gland.
EEiologic Diagnosis: Viral pneurnnia, hepaEitis, and adrenalitis.
Etiology: Corpatible with Equine HerpesVirus-l InfecEion.
Corents: There $rasBeneral agreenencwich the contributor's diagnosesand
ccrnenEs. Tbe morphologic diagnoses lisced rverereachedafcer discussion of che
- 4 -
lesiqls and further consultatior.
other equally accepcablercrpnologic diagnoses
prior to the conference iircludeh necroci"ing-Sq'e
giy:1"lt_participants
pi.,-onniEG,
necrosis,
necrotizing
and
adrenalicis.
parricipancs
3l:tf?::l,h:!.Eo"ellular
arso listed acr acute to subacute.plegritis, and RE-cell hyperplasia wiclrin che
liver.
Featureg of the lesiqrs ii ttre lung were characterized as diffuse rcderaEe
to severe qo"g:lliol
of 5h9 pul-mrary pa5enchyr-ra,rhe consolidared appea;"*;(cctsidered almost normal
foi fecal i_qngl, ai-ffuie edeuraof cr,.-rivSi;;-;;L,
cellular infittrates
cqtsisting.of Plq{'F'and macropn4g"swithin septa and aiveoiar
spaces, necrosis of alveolar and snall bronchiolar'liiling cells, da-rG p;;*""
of large-nr-der of eosinophilic intranuclear inclusion bodies in several cell
!IPes:- lesions in the liver consisted of nrlrifocal necroii" eo"i *iin
distributiqr,
infiltration,of
pr_imariiy
inro it""" foci srd "-i"oa*
the
presence of occasiaral.eosiropiritfc lnlranucleir
".rt".ptrils inclusion bodies aE rhe periphery
of cheselesicrs. Lesionsin'the adrenar.i"-a""ciiu"i-"r
andnecrosis with mineralizatiqr. Therewascoql"c" f""[ *l;#;;1-i;#il4.
;f;-i;ii"il;;;i-'
resPonse
within this tiesue. Eosinophilic intraiLrclear inciusiogre
and wererentioned by.o.nfr a- few partrcipanis.- several p.iii"ip"nrs ,{e".
"oi-'cDvro,s
@nrioned
foci
of hypercellularity wi*riir the paienchG;i-ihe liver wirh cells described
as
-Ttrese
foci'lere iicerpiei"d ioci oi ;h*duiG;tff*11^g:ecur_sors.
nsnatopoiesis !*lich is considerednomral fbr
fetal"sliver. ftre pattrog;n-sis of rhe
--virar
lesiqri in the fetal nqine
aiscussea.
infecrion of rhe ferus is
cqtsidered to be via idfection
"asof circulating i"-"f rtrpdytls
r{hich cross rhe
placental barrier and gain access to the mat5rnal circuiatidn and
retuf,n to the
fetus. Fetal tissues ire theminfected-*il[;L
virus chrorgh infected feral
lyuphocytes.- rtre ability of rhe foat io **i-;
i"iid;ffi
,"rponse is presenr
ln the last half of-the pregnancya1a qrgJ
tG-ipp"rlnr iirtramatoiiinfiltrate in the affectid Eissuls in rhis "*pt"i* me speilii.-'"ur"
of aborrim
maybe rediated, through elevated fetaf-cortirof
""5". levels lna Uieatdoin of placenral
atcactrents with.subeequentexpulsiqr of the fetus or prematurebirth.
Foals born
live are weakand usuaity di:
a8 houis. Th; virus irrsr courly isolared is
ln
Equinellerpes virus 1. funt e is not reported io causeabortion, ard EIV is
3
noc
isolated comnly in aboltims but can ira,.,s"abortiqr.
--v"""i""ri*-oi-'thi',Jr5-frit,
EHv
I
produce
is
kncnrrn
Eo
respiratory disebseand/or abortion_in horse"-(rj.
equine rhinopner-uutitis vaccine-(a fact drirte.i irm ah.-hi;;;;yi-"*-.h.-"u"ftc"a
route of infection. older Ex{VI vaccineswere deriuJ it*r-ttrt".
cerl line and
have been reported
to be abortigenic (1,2).
cqrtributor:
B.C.M.A., veterinary Laboratory, Box 100, Abbotsford, 8.c.,
Canailal-VZS-4i[&,
References:
r. Eagresoe, M. D., et a1.: Equine Herpesvirus I Infeccion in
vaccinated wich a live-virus rhinopriermanitii vaccine, attenuated in mares
cell culc're.
Can. Vet. J. 20: L45-L47, Lg7g.
of_fetal adrenal cortex to congeniral
. ^ 2. -Osborne,B. I. et al. :
infecrion. AE. J. oB/GTxLL4t &:po*"
622:627, L1TZ:--- 3. furdl,-C.-!__": "1.:, Equine rhinopnet-writis vaccine - Im.nngeniciry *rd
P$:ty in adult horses, including pregnanr rn res. Am. J. ver. Res. 39; tji:igi,
L978.
-
TI}OIHY P. O'NEII.L
Registry of VeEerinary Parhology
Departrent of Veterinary Parhology
- 5 -
Histories
AFIP We&resdaySlide Conference - No. 16
28 Januarv l98l
CaseI - 80-1038 (U58453).
earale poodle was presented 10/17/7-9 wicn nystagmjs,
clitticurEy wiEh aubulatiqr, aod a iectal terpirarure of I04.2oF. She ias Ereaied
repeqtg$]y.ryllh gentaqycin
qqd dexpnrethas6rewirh no iry.ou.*ni.
-She
A head-tilr was
noted LLlL4l79.
wae referred to a University Vererh;ry SAool for
evaluatiqr. Sltght.narroling of cervlcal vertebiae Z-g-;d'34,
with no evidence
of hernialimr was detrDnstraEedradiologically.
No other abnormalities could be
-fotnd. Disc fenestration was perforred"frort2_3
co cs-0. ten aays-i.t"i,-afte-r an aPParent normal recoviry.fror surgeryl-itre-a"fHi"a-""dderfuy.
nauies arn
'rD'r vacciiatiqrs
had been adurinistered rJurinety in frct,-Lgig.
case II - 0065 (AFIP 175ggy+).
HlsEory: -Ulceraclve lesidt rnasuring 2 cm in diaeter remved fro the
|l:":-i-""
,99:io," of frart fpg of 8-year-o1d nale cat. origin of lesion ir-,mf..roo"t
DUECnOUghE
to be due to a bite wourd fron anocher cat
Case III
- A28109
1665535
afa
aperitoreal
Case rv - AT-1367-79L758/,62
rfrl an
resP
lled and necropsied 14 days after receiving a
experiuental coupornd.
curkey wirh history of recenc ill-defined
TINI}T'HYP. O'NEILL
Captain, USAF,BSC(rc)
Regiscry of Veterinary Patholory
Deparmrt
of Veterinary
Pathologl
Resulcs
AFIP WednesdaySlide Conference - No. fb
28 January l98l
Ca s eI - 8 0 - 1 0 3 8(U 5 8 4 6 3 ).
ennle podfg was presencedl0/ L7/7g wich nyscagmus,
ditticurEy wich ambulaEion,and a reccal temperacure
of 104.zoF. She iras Ereaie<t
repeacedly.ryl$ gentamycin
qnd dexarethasonewich no improvernnt. A head-Eilc was
-She
noced LL/L4/79.
wbs referred co a universiiy veceiilrt-Silool
for
evalr.ntion. Slight.narrowing of cervical vertebiae
2-3 *A"f-+, wich no evidence
of hernialion, was denrcnsEraEed
radiologicaffy. No other abnormalities could be
found. Disc fenestration was performed"frqr c24 co cs_0. Ten days rater,
recovlry.from
aoftiea-s;aaeiiy.--n"uies and
,l,t!:,T
lpp":g"t normal
"lJfIL" -1l
vaccinaEions
had been adninistered "urg".yi-ine
roucinery
in March Lg7g.
Concribucor'sDi
is_& Cmrreqts:, Granulomatoussnningoencephalicis.
visceral Eissues were suoniEEed.
Lesiqts were limited to the brain. All slides may
loc deupnstrate represengagive
lesions-of meningitis. Special stains (PA^S,
Gran) failed co despnsrragean
etiologic.agenE. Alchough-the.spinal cord was not available for examinatior, che
brain
braln lesions
lesions are
are compaEi
compaEiblewich the described entiEy---canine granulomacous
sranulmnrous
meningoencephalcrnyeI i t i s .
AEEendees'Di
is & Connents:
nnderaEe, cerebral cortex
, poodle, canine.
Eciologic Diagnosis: Canine granulounuous ireningoencephalonyelitis syndrome.
Etiology:
Unkncr'^rn.
Cmnents: The rnrphologic diagnosis was the r-rnanitrpusconcensus of rhe
attendees. The preconference etiologic diagnoses given by participancs enconqrassed
canine granulouratous encephalornyelicis and frinnry"recicuiosis
of lne
nervous fIstem. The features of the lesions consisced of mulcifocal granulmatous
"""ii"i-lesions locaced wichin the white matEer of the corEex and to some exc6nc che gray
matter, the internal capsule, ffid hippocarnpus
depending ox che sectiqr.
Ueniigebl
-Perivisculai
involvenent was evident in sqne sections.
discribucion was prourifrenc
in many of the lesiqts.
For the rrDsc part the lesions erere noc accmpanild oy
changgq in che adj-acent neuropil but smre atEendees reported foci of iarefaccion
and gliosi"
Cell cypes
|n addition to.Eha granulmatous meningoenlephalitis.
involved in Ehese lesions included rncrophages/hisEiocytis,
lyrphocyc"", pi"sr"
cells, &d occasionally neuErophils. Tha macrophagic/irisciocyric cbffs'wire
described as blasEic appearilg by a few parciciianls,
and nicocic figures were
reported by a few. A reuiculun srain wai projelted that denrlnsErac"Ediscontinuous
silver-positive reticulun fioers around vessels and dissecting Decweenche cells in
these foci.
The discussioir then turned Eo che etiologic diagiiosis of che lesions
present.
of lesions-as "canine granulouratous meningoencephalomy^'icis"
t"ignation
denotes an inflarrnaEory nature of the condition whereas prirnry"reticutosisinfers
a neoplascic involvement. Sone attendees who listed granulunatous encephalicis
sug4ested a viral etiology, but viral particles have noc been reporred wich trris
entity. ,Special stains by the contributor and AFIP for fungal-, baccerial, and
procozoal organisms were negaCive. It was poi.nCedout Ehac chese two entities ilay
in facc represenc cne sainecondicion Dut in different scages. Tne infiltracion cf
rhese inflannratory/neoplascic cells is considered co be from tne circulatirg
npnonuclear phag,ocycesystem. Hiscogenesis scudies of che brain have sholn crrac
macrophagesand possibly microglial cells arise fror chis sare sysLem. llicroglial
cells do not appear in the brain unEil afcer vascular penetration occurs, and
autoradiography scudies have shcwn that macrophagesmigrate co perivascular spaces
in Ehe brain.
This disease in dogs has been conpared uo che condition in man
terred reticuL-m cell sarccrna of the CNSwhich also is a disease wichout a known
etiologic agenE. Correlauion beEweenthese Ewo condiEions has noc been establich'r,"1
This disease condition in dogs appears Eo involve two age groups: young dogs l-2
years of age and dogs over 6 years of age. CorEicosteroids are reported to
signs
alleviate signs in affected animals but death is inevitable.
Clinically,
mrsE be differentiaLed
fror ocher diseases involving Ehe CNS including other
inf launatory and neoplastic conditions.
Cqrtributor:
Veterinary-sox
Diagnostic & InvesEigational
veceiiffiT]-IEilidine, P. o.
Laboratory,
College of
1589,Tifron, GA Stlgt+.
References:
I@.G.etal.:Granulomatousgpningoencephalmyelitisinsixdogs.
JAVMA
172: 1195-1200,L978.
2. Cordy, D. R.: Caninegranulmratousueningoencephalmryelicis. Vec. Pathol.
L6: 325-333, L979.
3. Russo, M. E.: Primary reticulosis of the central nervous syscernin dogs.
JAW'IAL74z 492-500, L979.
4. van Furth, R. et aI.: The mononuclearphagocytesystem: A new
classification of macrophages,upnocytes, &d uheir precursor cells. Bull Wld HlEh
Org. 46: 845-851, L97'2
CaseII - 0065 (AFIP U58834).
measuring 2 cm in dianeter removedfrm Ehe
anceFl6if-p-ortion of front leg of 8-year-old male cat. Origin of lesion is unknor^n
buc thoughc co be due to a bite wourd frorn another cat.
dermatiEis.
is {i CourEnts: Diffuse pyogranulomaEous
hages are morphologically compacible wicir
Trichosporon sp. Futher evidence for che identification of tire organisns as
reans of
for Disease
Disease Control
Control by reans
frmr Ehe
Ehe CenEer
CenEer for
obtained frmr
ElT was
was obtained
friffiosporon
frffii#Foron Sl
Contributor's Di
ffiodycechniquesusingspecifica11yIabelledanciserumappliedto
deparaffined cissue sections.
Attendees' Diasnosis & Conments:
is, pyogranulCInatous, cnronic, focally
exEensive, moderate, subcutis and skin, cat, feline.
Etiologic Diagnosis: I'tycotic derroacitis.
Undetermined-by actendees (see contributor's coimenEs).
Etiol{y:
Conments: The nujoricy oi che attendees lisced pyogranulomaEousdermacicis as
their preconference uprphologic diagnosis. Sonrepreferred a granulmntous
derrnacitis as only a few neutrophils were pr:senE in Eheir secEions. Tnere was
- 2 -
general aBreerlentwith the etiologic diagnosis as a mycotic dermatiEis. The
etiologic diagnosis given by manywas sporotrichosis with differentials chat
ircluded phycomycosis,helminthosporosis, chroronycosis and phaetrtycosis.
Protozoal dermacitis also was urentionedas a differential diagnosis. FeaEuresof
che lesion included breardownof collagen in the superficial dermis and
infiltration
of che deep dermis and subcutaneoustissue witn a mixed cell
populaEior of histiocytic cells, lyurphocyces,plasma cells and depending on tne
sectitrt large or snall nunbers of neutrophils. In addition Eo the infiltrate,
necroEic cellular debris also was present. FeaEuresof che incra and extracellular
cigar shaped,organisrnsincluded buddirtg yeast forms, chains of organisms, and forre
suggestive of hyphae. A GT'IS
and PA.Ssubscanciated chese findings. Sm atcendees
observed fine filmentous processes at che ends of the organisms and chus che
possible confusion wiEh protozoaL organisre. A few partiaipants and ocner
consultants wifhin the Institute did not feel the organisms-werecharacteriscic of
sporotrichosis.
Features inconsistent with sporotrichosis included the large
nLnber of organisuls present, cbains of organisms, cigar shaped forms which r.reretoo
long for sporotrichosis, aod hyphaewith apparent brtnching. Sporocrichosis mre
closely resembleehistoplasrosis in H&Ecissue sectiors, and the presence of large
nrrrbers of organisms is uncourrDnin sporotrichosis.
In the discussion of tne
inrrunologic-findings in this case,. several attendees wonderedif cross reaccivity
with other fungal organism occurs. Cautiqr vras expressed thac diagnosis carurocbe
bqsed purely on the rnrphologic appearancein tissue section. Clinical pathology
also is iryortant.
This case is considered tnrique in chat deep dermal involverenc
of Trichosporon has noc been docrmented. One case of disseurinited Trichosporon
nycosis was alluded to in one reference (2). Trichosporon is reported co involve
exclusively the epidermis and hair shafts of man, horses, reptiles and a spider
ronkey (L,2). Trichosporon beigell! is the causaEiveagenc of wtriEepiedra in
man.These1esffichehairshaftsaidresemb1epaiasicicnics
grossly. T. cutaneun involves both the epidermis and hair shafcs in man. The
agent idenETfii&ITi-The single case in the upnkey was T. cuEaneum. The organism
(l).
rJported in reptiles.was f .""p..i""
Tne organismAfeffiTbEf-in horses"was
designated as T. equinun.Concributor:- Veterinary Diagnostic Cencer, University of Nebraska,Lincoln,
NebrlEE-a---585&ll
References:
-ffins,
f.
C. W. et al., Edicors: Black Piedra, whice piedra and
trichorycosis axillaris.
IN I'4edicalMycology, Lea & Febiger, Philadelphia, L977,
pp 181-184
2. Kaplan, l^1.: Piedra in lower animals: A case reporc of whice piedra in a
rnnkey and a review of the liceraEure. JAVl,tA,U3-1U, Feb f959.
3. Kuttin, E. S. et al.: I'tycosesin Crocodiles. GrosseVerlag, 1978,
pp 39-48
- 3 -
- A28f09 (AFIP 1666535).
lled and necropsied 14 days afcer receiving a
single intraperitoneal dose of an experimencal coryourd.
CaseIII
Cmcributor's D
is & Coruents: I6.rlcifocal granulcmatousnephricis
t crys
material.
Cgrencs: -rt appears that the experirencal coupoundor a metabolite has
precepicated wirhin che renal tubules and elicited a responsesimilar Eo rhac of a
foreign body. Although precipitation within the renal cubules is noE rlncosrt)n, crte
granulontous reaction is, in our experience, unusual.
Actendeesr Di
is&
icis,
granulomaEous, rultifocal,
rcderate to
EEiologic Diagnosis: Chronic toxic nephritis.
EEiology: Undeternined.
Cou|9ncs: Other preconference nrcrphgfggic diagnoses given by sore participancs
were necrogranulwetous nephritis and tubuto-intericitial-nephritis.
Tire diagirosis
Iisted was considered to encourpass
all of the changespresent in the Eissr.p aid was
not restricced to jusc- tubular and interstiEial iniolvi+urent. Changesincluded
-i"i"r"iitirf
mulciple- foci of granulmratous,inflasnarion in the coruex and reAuTia,
and cubular heunrrhage primarily in the medulla, and minimai 11g1"'-t"iory ctranges
in the pelyis in soue sections. Granulomacousinflarrration involved i;i
;i-:
tubules and interstitiLul in the cortex and medulla and occasionat giq,"rufi.
A
mild subacute infiltrace algo was present in Ehe pelvis. Soue
reporCed
hyperplastic regenerative changesircattered in coitical cubules.
"tcE"O"".
Anocher fiature
reporEed by -somqwas Ehe presence of casts and mineralized material in lrrens of
corEical and redullary tubules. The inflamratory foci were described as singular
and coalegcing infilcrates of macrophages,lynph6cytes, neurropfriis, pf".* E"ffr,
and occasional multinucleaEed foreiin Soay type giinc,cells.
tn" center of manyof
these foci conLainedbirefringent ciystaliini:'maEerial.
This material-"pp""r"a
faint greenish structures in nonpolarized light. The polarized material'was
"t
suggestive of calcir-unoxylaEe crystals. The-eciologic'agenc(s) speculated by msc
part.iciPanEs lras rhe experimental ccurpoundand diffErenclally inciuded variols
antibioEic, cotrpoLndsand heavy uetals. The exact nature of Lrre compotrnd
was noc
disclosed by che conrributor.
Histochernical analyses included microincineratiar
gnd spegial stains for uraEes and calcir-an. The risults suggesteda mixcure of
inorganic and organic deposits-in che Eissue with a scronglsitivity
for calcirm
corplexes and a weak reaction for uraces. Ttre disapp.araici of biretringeni--lncetial.ltPtrt microincineraEion indicates me preseirle of organic deposiEs.
Further discussion included the pathogenesis of trre lesions. Ic was speculated
thac- darnge Eo Ehe tubules of nephron-could have resulted in the deposition of
insoluble uncabolites of the conpoundor products of cellular degrailacion in uhe
inEerstiEirm ffich subsequentgranuloratoul response. It was sur[rising chat only
selecced comPonenEs
of the cortex and sEdulla were affecced and not the encire
as
is
expected
wiuh agents causing toxic Eubular nephrosis. SomeatEendees
fi$ney
teIE the glouerular involveuencwas due co extension from prirnry cubular lesions.
A suggestion of irmune ccnplex disease also was enEertaineaUy the csrcributgr
(personal cmnr.nication), due co breakdormof baseuenEmenbraneswith exposure of
antigenic substancesand subsequenE
inflannncory response.
Concribucor:
Pfizer Inc. CenCral Research, Groton, CN 063/+0.
- 4 -
CaseIV - M-1367-79 (L758462).
week.oIdcurkeywiEhhisEoryofrecenc'ill-defined
respiraEoryrdiseasein flock of 5,000
IfboJacory Results: Electron microscopyof organismson epichelial surface
reve@
attactrenE zones cbirsisrenE wich Cryptbsporidia sp
Concributor s Dj.Hngsis & Cqlrpntst
Respirarory cryptosporidiosis.
ractir ized "Oyc'trionil infl"tr'*cory
chickenilg of tne lanina piopria, mlcosal epichelial'protiferacive charlge'an'J
deciliacion, moderate glanduiar attenuation'and rhe piesence of epiEhelfal surfaces
of large nuurbersof protozoan organistrs consistent mirphologically wich
Cryptosporidia gg
Actendees' DiaFsrosis& Courrents:
is, subacuteand proliferacive, diffuse,
.
moderate, trachea, turkey.
Etiologic Diagnosis: Respiratory cryptosporidiosis.
Etiolog5l: Cryptosporidia-sp.
Comerrts:@gree[IEnEwichrhecontributor'sdiagnosisand
c<xments. HisLopathological-features cmenced on were che diffuse thickening of
the tracheal mtcosa, Lhe infiltration
of the lamina propria with inflAnmacory
cells, the formation of cystic structures lined by ciacheal epichelirm, and tbe
pr9s9n99of nuunrous small round organisms along tbe ciliated'borders of che
epichelir-ur and_free in cystic spaces. The inflInrmatory cells consisted of a mixed
populatiot of lyurphocyEef
, pfasriu cells and heEerophifi. Features of uhe organisms
in H&Esectiqrs lrtere_
variability
in size and shape-and vacuolation of ssn f5rms
with dense granular bodies to one side of the vaiuole. Parasitophorus vacuoles
also were present.
The encire life cycle of Cryptosporidirm sp. is unknown. D.reto iEs small size
(2-5micrcrs)itisoftendiffientiateEhevariousstagesintbelife
of the trDst probable life cycl6 (noce: we
fYcle. Ttre follor^ring is a conpendir-ur
knowCryPcosporidiun is a coccidian so we should cimpare what we see wich nrhacwe
kno^lffihercoccidianlifecycIes,i.e.lnimeria,Isospora).Tnemode
of infection is unknownbut ic is believed due to inge6Effior
pos3l6ft
inhalatiqr) of sporulated oocysts. Sporozoices probably escape Fror che oocysEand
then infecE epiEhelial cells. There is difference of opinion-as ro whecherthe
trophozoites (the infecting sporozoice) are intra or exlracellular.
Eleccron
micrographsshow four layers (i.e., milnbranes)surror-nrdingche parasice and chus
manyfeel Ehis is indicative of trpo inner renbranes of che proc6zoa and rhe oucer
snslbranesof the epichelial cell.
It is Ehought the sporozoite burrows right trnder
che epithelial eell mesbraneand thus is incracellular.
The subsequencsuages of
the protozoa'ate similar Eo that seen wich ocher coccidia: Trophozoicesgive rise
to schizonts (wiuh eight nnrozoices due Eo three cell divisions), these rupcure and
give rise to other merozoites or gauEtes. 0ocysEs have been reported by a few
wrcrkers. Micrographsare not consistent with that of oocyscs, however,and Enus
chey nay be schizonts, eicher inEracellular or passing in feces due to disrr'- tion
of epichelial cells.
IE is suggested, however, that oocyscs do form, are passed
ouL in che feces, sporulate, and are chen infecEive to other hoscs.
- 5 -
Crypcosporidiosis has been reported in a varieEy of species including repciles,
qurkeys, geese, mice, rabbits, guinea pigs, laubs, calves, swine, unnkeys, and oan
(4). Diseasesare noE always associated wiEh chese proEozoa. They i.nhabic che
intestinal Cract in rnst of Ehesespecies.
CorrcribuEor: Animal DiseaseDiagnoscic Laboracory, PurdueUniversicy, WesE
Lafa@l3N-4i7907
.
References:
f.Tr
. G, & Srich, t'1.D.: Crypcosporidiosis in man: Parasice life cycle
and fine scructural pathology. J. Pach. L32z 2L7-233, 1980.
Z. Fletcher, O. J. et al.: Cryptosporidiosis of the Bursa of Faoricius of
chickens. Avian Dis. L9z 630-639, L975.
3. Hoerr, F. J. et al.: Respiratory crypEosporidiosisin turrceys.
JAWIA173: f591-r593.
, 4, Potrlenz, J. et aI.: Bovine cryptosporidiosis: A transmission and scanning
elecEron microscopic study of soe sEagesin che life cycle and of the hosc-parasite
relationship. Vet. Pathol. L5: 4L7-427, 1978.
TIMqIHY P. O.NEILL
Departrent of Vecerinary Pachology
- 6 -
Histories
AFIP Wednesday Slide Conference - No. L7
,
t+
February 1981
Case I - 0[O020I
P 1758400
Enale BHErat lras lg!,using
her hind legs for about
:.^1"
jidsr';p:*
.6T'3.flf ;3'H
5ffI;"315"Ti
cm,
: fi1nly
rm,i'ff]"[::
H']*
it'i"
a*ached ro
rbe dorsal
i.ea of En"'UIseof
weekl
a n r l uoderately
rrnJo-aFalrr
f.i*
tan-gray aqd
firm.
fnn-oraw
iiri"'"ii "iLIia,
4r-^
&-tle
tar.r-giayrissui exrendedi"co-itJl#"i,,;
mlscles.
mtSCIeS.
Ttfg right
fight
Overv
4-0 cm
in-.liinnor-oo
nraanii*u
^-r ..---- .cr-.
ovary was
cm in-di"ttBter,
creflFpink
.The
and
very firn.
ryas4.0
Siutilar tissue, 2.0 cm iir diamtet.
enrbedded
rhc caudal
nnrrdnl pole
rvrla 6f
^F rhe
rho rigtrt
in rLre
, was enbedded
kidney,
-
and rourd masses,0.3
1.6 cm in-aiarei".l
,*r"J;;h"'i*g;:
case II - PA-089-e90,__E0:989-2r-!0
(aEw L7i737g).
three day course of
There was a
recnargy, <lepression, anorhexia, nasal diicharee and sqle
" ' " v@ition.
etransient response to supportive therapy.
Laborator
IÎBC- 10,900
Pt'N - 607"
Band - 2%
Ly'mph - LL%
Eos. - 27"L
- aL friset of initial
PCV - 31%
signs
CBC- 5/L/80 - prior ro dearh
l'{,Bc,- 220
PW - 24%
Band - 1%
Lyqph - 25"1
Eos. - 5A"L
Gross t'lecron54$1114
segtrEntal encericis, *ell
bowel, characcerized by
.
Eurglo sh,otlen loops, &d patchy
areas of serosal reddening.
,
#
CaseIII - ? (AFIPL757'295).
cisstp massin
-,-Tltcotyi Tissue from a wild caughtrabbit wich a subcucaneous
Ene lngulnal
region.
HISTORY AND I"ABOMTORYDATA:
8-25-77 - A 5 month old female lrish Setter waa preaented to a practicing
veterinerian
wlth chief complainte of lethargy and anorexia.
Rectal
PCV
Fecal
temperature - lOOoF
- 20%
- hookworm OvA
The anlmal was treated
with
dinitrophenyl
(Dt{P), anpicillin,
& a vitamin
eupplanent.
9'7-77 - The anlmal was returned becauee che was depressed, vomltl-g and anorectLc.
Auscultation of the thoraclc cavity revealed pulmonary congestLon.
Temperature
SMA 12
PCV
wBc
Amplcllltn
-
104oF
normeL
30%
25,000
treatment
contlnued.
9'L4'77 - Temperature - L06oF. Anlmal was provided ftuid
(4 mg/kg b.1.rl. firsr dayl 4 ng/kg daily thereaiter).
therapy and G€ntamlcin
9'22'77 - Temperature dropped to t0loF but no lmprovement in general condltlon
of anlmal was noted.
Anlrnal was glven a blood transfusion and Cepholosporln.
9-23-77 - Anlmal' was found dead and necropsled by practiclng
veterlnarian,
reported whlte plaques on parletal and visceral pleural gurfaces.
DIAGNOS
IS:
Tentative
clinical
DX:
Systemtc mycotlc infection
T I M O T HPY. O ' N E I L L
Capt., USAFB
, SC(VC)
Departmentof Veterinary Pathology
who
ResulLs
AFIP WedndesdaySlide Conference No. L7
4 Feburary l98l
Case I - 0100201(eilp U58400)
le BHEraE was noc using her hind legs for abour a
week, but was argrg
The animal was killed.
A very firm,"gray-pink
3n9 :"!ing.
prorrusion, 2.0 X 0.? X 0.5
cm, finmly aEtache<lEo che dorsal ir"" oi Etre'bise cf
Ehe tail was observed. The ventral lisrbosacral spinal
*"r diffusefy enfargea,
Ean-gray d *9"lately
"."" inro the adjicent
firur. The tan-gray cissue extended
mrscles. The right ovary w.1e4.0 cm in-dihreter, cre€rrFpink and u"ry ii*.
flTirar tissue, 2.0 cn in diameeer, lras embedded'inrhe iirO"i pore-6f-ine rignc
o
kidney, and round masses,0.3 - 1.0 cm in diaeter, $rerein the ltrngs.
cqrcributor's
Diagnosis & conmencs: osteosarconna.
ae-iiorcfre
mass of che right ovary. Normal
righc warian tissue could hardly be found in che secrions. efl Efran!""-io*J-"c
necroPsy $tere osteosarccna. The primary siue of rhe neoplasmwas rcsE likety in
the vertebrae of che lmbar spinai coh-mm.
Arllqndees' niagnosis & ComrEnts: OsEeosarcqn.
cdllErlts: There was ttnaniunusagresnent with the contEibutor's diagnosis.
Osteogarcons are considered rare in-the rac. Experimencally these tus'rs have
- pen produced by certain cheuricals, radiaEion, ."3 uy r"ad;i;i
r.".t" (ii.
Osteosarcmas have been induced by inoculation of rats with ihe t'taloney'lirco,"
virus. Ttris virus is ancigernicaliy igdiscinguishable frour Harve/"-s.ico"r"-uiru"
of racs. TLrel4aloney.sarc&ra virui also is leferred ro as the Mlloney murine
leukesria virus or mriine sarcqna virus.
_Tunpr produccion wich tEis-uir""-is-rapid
and occurs in approximately 80%of inoculated
rics.
Ocher tr.Ercrsremrced in rats
inocurated wiEh this vi-rus are plasmacytmrasard fibrosarcour,as(4). 'This sase
,vfrus also has produced fibrosaicwras in newborn hnnlscers and rna6dmyosarcouas in
mice.
OsEeosarcqrasin the rat have been studied as an animal updet 6f tne hrrtan
EurDr, as there is similar distribution in both species (1,3).
a
Contributor:
Searle Laboratories, Chicago, Illinois.
References:
T-iffi,
N. N. et a1.: Ttsprs of tne bqre. In patholoeyof Turprs in
lehx4tory Animals,Vol. I, part I, IARCScientific pubfffi
pplt69-T/-2. MisdorP,W.: Skeletal osteosarco{na.l6del No. f98. In Handbook:
Animal
I'bdels of Hr.man
Disease,-Fascicle9, Registry of Ccry. path., m
1.: 0steosarcofo. Ibdel No. It4. in n"naUok:Aninal
I'{cdelsof HrmanDisease,Fascicle 6, Registry of Cmp. path., m
-F--
@a1.:Turcrs.tneatho1ogybft,aboratoryAnim.eIs,Ecl.by
Benirschk€, K. eE al. Vol. II, Springer-V
i2r2.
case tt - pA-0d9-r]-d0, p4:0gq-2t-30 (A[Ip t7>73/,t1.
:fter a cnree day course cf
lechargy, dcpression, anochexia, nasal discirarge and srxnevr:oriEion" Tnere rJas a
iransienE response Eo supportive cherapy.
wtsc-
- aE onsec
10,900
PCV .
PI'N - b0%
Band - 2 L
Lynph - 1r7!
E o s . - 27"/"
li*.nt.t"l
signs
CBC- 5lL/8O - prior to deach
IIIBC- 22O
PI\il - 24%
Band - 17"
Lruh - 25'L
Eos. - 50%
^ 9loss TgcroPsy Lesious: Segrental enteritis, wnll bor,lel, characrerized by
curgld sTdorrenroops, Etrod
patchy areas of serosal reddeni4g.
Concributot's Diagno?es& Cosmrts: l) EnteriEis, characcerized by epiEhelial
cell
ion and plugging arci rppnoid hepieci;
(smle secEims), subacute, diffuse, severe, snati iiEesEine, canin". 2)'Bone
marroir hypoplasia, nyeloid and erythroid, difl'use, severe, ieu'1r, canine.
Ccnupnts: The gross and microscopic fin<lings are characteriscic of canine
parvtrvirus infectiqr.
Such lesiqrs aie similar-to Ehoseof feline panleukopenia,
ircluding a relacive increase in nurbers of eosinophils in tne otneiwise
rr:anulocyte depleted bore marrohr.
Atcendees'
icis. necrouizing r^ri.thvillous atrophy,
rrluntirg and fusion, subacute, nnrlrifocal co diffuse, moderate to severi,- small
inEestine, mixbreed, canine. 2) tyrphoid deptetion, diffuse, rnderaEe, peyer's
patches, small intestine.
3) Depletion, erylhroid and myeloid, diffuse, slvere.
bone rnarrow, Iong bane.
Etiologic Diagnosis: Viral enreritis.
Eciology: Canine parvovirus.
Cqments: There was unaninrcus agreement with the concributor's diagnose".
Attendees agreed with the cqrtributoi's
conparison of rhese hisEopathol5gicaL
i.,rsiqts to those seen in feline panleukopenia. Several participaircs renEianed Ehac
'.lhole body radiaEion can prodrce essentially similar chaiges in'the intesEinal
nucosa. Participants-preferred
bore marro{d depletiwr to hypoplasia based on ine
assutptiut that noroal bare marrot{ cellularicy
was present at- are tine versus a
develognental hypocellularity.
the tvn foran-sof canine parvovirus infectiqr r.rere
tliscussed, nrynoly the nryocardial and enteric forms. The-myocardial form is
reported to occur in dogs less than 8 weeks of age corresponding uo Ehe develcping
'nyocardit-ur. TLre intesEinal form occurs in all age groups of dOgs and varies frm
inapparenE to fulminating herorrhagic enteritis.
Hiscories of dogs with canrne
- 2 -
Parvovirus infection often include recenc boarding, kennel exposure, or dssr-)ciacion
wich other infected aninrals. lt"ly reports descriEing th.-"ii;i;;l-i".i-p"i.origi""r
findings of canine parvovirus infeccibn have been generated in the pasr tou y.!r..
Contributor:- UniversiLy of Pitcsburgh, Central Animal Facility,
PA F26r:---
References:
r' uarpenEer, J. L- eE al.:
pircsbtrrgn,
i-ttF----
Intestinal and cardiopulur,rnaryiorrns of parvo,.,irus
infeccionin a lirr"r of pupr. JAwrA176: L26g-l;i\,"I;io-""'
HisEopachologicar-"uia"n""-eo.
. .2' Pleucher, J. M. et-ai.:
parvovirus infecriop
F- - ' - - - e- - in d o g s . J AV MA
L 7 5 : 8 2 5 -8 2 8 ,L g l g.
3' I^loods,C. B. eE al.: Canineparvovirus enceriEis. JMfIA 15: L7L-L1),
19g0.
- ? (AFIP L757295).
.HlsEorv: 'l'issue from a wild
caught rabbit wich a subcutaneous tissue urass in
the EffiAf
regio'n.
Case III
(hrtributor's
t'tult
Diagnosis:
subcutaneouscoenurosis (Coenurusseralis) of
Attendees'
fr.rrpnerogrc.uragnosls: l) coenrurus,subcutis, rabbic .
g::::l-g::::,
granulmatous,
I::*lt
focally
exrensive,underire,
,[i".
"ubcuii",
extensive, skin.
z) cellulicis,
-i)'pannicutiris,
J/' rg'lrrvurrLrDl
Etiologic Diagnosis: Coenuriosis.
Etiology: coenurus species probably coenurus serialis.
courents: The parasite has i cegw;nEffi-rifriEifif6bdy
wall. The Eegr-unenr
has microvilri co iicrease surface aiea for absorbtion oF
nutiiencs. The body is
filled with a loose parenchyuraand has no
or pseudocoelqr. The parasite
is
.
thus identified as a plaryhlhinch.
"o"i*
The rrso
*:oi ei;F-;i-p"rasiric
placyhelminths are tresratbdesand cesiod"r.- itt" parasrEe
can
fron trsnatodes since it contains no aigesiive Eract but does be differenciated
concain calcareous
corpuscles eobeddedin the parenchyma(fost nurnrous in rhe
nect
retioni:--ih.'
parasite is thus a larval cistode.' Present
ntr="ous scoreces in'vigiroci.[ rr*
a bladder; -theseare characceristics of a "i"
Each scolex has four suckers
and a rostellLm wiEh nutrsrous hooks. rne Jcoreces
"*n*ur. are attached to the bladder oy
a
necf , Specific identificaLion of a coenurrr i" *t possible on histological
section. lbere jl"
,?bgu!
l?."p."ies. of ltrtti""pu or coenurus reporred in cne
liEerature'
Although
hydatid
iys-qs.h"yg cysc walls and nu*rou" scoreces, chey can
be differentiated fiour Loenuri.- Hydaiid
mrch srnaller scoleces.
Unilocular hydatid.cysts-have very- chick "iri"'i,"""
laminated wal1s. Mrltilocular nydarid
9y"!q havevery thin walrs. thes6 scolecesrr"-oir*
gr;,rd-i;
ffigîl";";;;;
iryld".the larger.papent cyst.and thus do not mrpholof,icaity res-rrble a coenurus.
coenuri
are idencical to cysticerci in all ways eicept the *rftipf"
;*,b.;-;i-scoleces. rE is often wise co scep section drrougir utaoders and'counc che nrrnoer
of scolices present. rf only one is pruse.,c-il-i3 ct;-i;;;u";'ro."
chan one tr
coenurus.
Contributor:.
CA fiff;-
Synuex Research Laboratories, 340f Hillview Ave., palo Alco,
- 3 -
cAsErv - cp77-1159(AFIP1673404).
:
8-25-77 - A 6 no.ath old fa'npLe Irls Setter
veterinarien
wlth cttef.eorplatnte
of lethargy
Rectal
PCV
Fecel
temperature
Ttre aaLnal
nas tteated
wa8 presented
and anorexle.
to a practlclog
- IOOoF
- 2O7.
hookrorm
wlth
dleopbenyl
(Dl{P), arylclllla,
and a vitanln
9-7-77 - Ttre aofuul rtaa returned beeauee ehe wae depreesed,
rectic.
Augcultatlon
of the thoracLc cavlty revealed pulnoaary
Teoperature
SUA 12
PC\r
r{Bc
Anpictllln
suppleoeat.
vmlt{.g
aad aqocoageetioa.
- l0droF
- nor:nal
- 3U7"
- 25,000
treatment
contl.nued.
g-L4-77 - Temperature - 1O6oF. Ant-o8l was provlded
(4 ne/kg b.i.d.
day; 4 ng/ttg dally thereefter).
first
ftuid
therepy
aad Geotamicin
9-22-77 - Temperature dropped to lOloF but ao lmprovernent in general condltion
of ani-taal was noted.
Anfunal was gl.ven a blood transfusioa
and Cepholosporin.
9-23-77 - Aninal wae fouad dead and necropsled by praeti.cing veterinarlaa,
pleural surfaces.
reported white plaques on parletal
and vlsceral
who
DIAGNOSIS
Tentative
cl.lnical
DX:
Systeml.c rnycotlc
lnfectlon
Cantributor's
Kidney, acute tubular necrosis r{ittl
is. Kidney, rretascatic calcificaticr.
Crents:
Etiologlc Diagnosis: tilephrotoxicity due to gentunicin toxicity.
lbtastatic calcificatlqr
was pres€nt in the heart, lungs, kidney, and pler.na. The
mphologic changes in this case suggest that the aninal died in a r-nemic scate
resulting fro renal failure.
The renal lesisrs are nonspecific ard occur as a
result of a variety of nephrotoxic agents. Tfle renal lesiors are both acute and
fibrosis are
chrqric.
Ttre tubular regeneratiqr and imnature interstitial
gentaicin
consistent with fhe 14-day duratiqr of
therapy. AtEhor4ghthere are no
laboratory data to confirm our suepiciqr, we suggest that aneuria and/or dehyCrattmr
my have been severe enrugh to produce inadequate renal perfusion, thtxt iqnring
mrral renal netabolic processes and excreticr of gentamicin. Ttris may have
allo^red noruul lt:erapeutic doses of the antibiotic to accunrlace to toxic le..l-ii,
culninating irr rubular necrosis and uremia and death:
regener
- 4 -
Atcendees' Di
is, tubular, acute, di.ffuse, severe, witfr
severe ntltifocal
dystropic mineralizat.ion, ki&rey, Irish setter, canine.
2) Mineralization, retasEatic, mrltifocal, mderate, basmrt rembrgnes, kidney.
3) Fibrosis, incerstiEial, chrwric, utlcifocal, mild to mderace, kidney.
Eciologic Diagnosis: Nephrotoxicosis (toxic nephrosis).
Etiologtrz: Gentmicin and cephalosporin nephrotoxiciry.
Qcnrnen63Tbere was general agremrt wittr cqrtributor's
diagroses and
cments.
Aminoglycoside antibiotics,rutt
notably gentaicin are krufii
nephrotoxins. Cephalosporins also are ne$rrotoxic. A cr-unrlacivetoxic effect of
these cwo antibiotics was suspected by nany of the attendees. Cells initially
affected in the nephron are Ehe proxirnsl tubular cells.
lesicrs also occur in the
collecting ducts eid parts of rhi distal tubules.
Gontributor:
SouffiElical
Divigion of Cqarative
t'Gdicine, Departrent of Patholog;r,
School, DalGe, Ti( 75?35.
References:
T:,['FI;T.
B. et al.:
@ nephrotoxicicy of antimicrobial agenEs.
NewErg. J. DCd. 296: No. 12, ffi3-670, 722-728, 784-787, L977.
2. -Hoqghton, D. C. et a1.' Gentqniqin and tobrarycin nephrocoxicity.
.
Arer. J. Patbol. 93: No. L, L37-L46, 1978.
- - _3. Spangler, W. L. et a1.: Gentamicin nephrotoxicity in the dog: Seqr.rential
light and electron microscopy. Vet. Parhol. L7z 206-2L7-,f980.
fiWffiY P. O.NEILL
Registry of VeEerinary Patholory
Depar@nt of Veterinary Patholqgr
- 5 -
Hiscories
AFIP Wednesday
11 Febtuary l98l
Case I - C-80-02
L757377-r.ras
slides
raE
one of a group of aninals on an
day of Ehe study.
19 IIt
y scudy killed on
Resurts:
raboracorv
g$i
3l#r:'??Jlf;:i:' re33
Case II -
-Case I
P L757415
-year
Pinscher --anorexia, emsis, obstipacion
Pacloo for
ark red
I
atory laparotmy revealed a dar k red. flaccid iler.m with dark
concencs. An inEussusceptior of the terminal ileunr was easily reduced. the dog
did not irprove and was euthanized qre week later.
!
wsss.
!^tr-vlqevl,
rstsrvbw,
Lvvwrve
e
sgL.\
Lvst
- 80-902 (3) (AFIP 1757303).
calf treaced for I r.reekfor pnerrnrnia and
enEerlcrs.
Case III
Laboratory Results: Cranial-ventral porcions of the lurg were dark red and of
----------&r
.
ircreased consis6fl
lfultiple small grey foci $rere scatteied throrg,hout Ehe lurg.
Case IV - 771L06 (AFIP L7570/q4).
'#
a 14 weetc old German shepherd dog.
Or April
4th
che anima.Iwas presented with vmiting and an elevated tqerature.
this respcrded
to Penicillin and Streptotrrylin Eherapy. 0n April 9ch the dog showeda sudden
deEerioration. The temperature was elevated, che hind lisbs and tail showed
partial paresis. The animal sloely iurprovedbut never regained full use of its
hind li.trbs. OnencnEh later it was fotnd Eo be rnable to walk and hyperaeschetic.
TII'MHY P. O'NEILL
RegisEry of VeEerinary Pachology
Departuent of Veterinary Pathology
'---r
^$4|it:#'r---_.|...l#L@
Results
AFIP WednesdaySlide Conference - No. l8
Ll February 1981
Case I - c-80-02
slides
ma
killed
AFTPL757377
raE was one of a group of animals on an acute
qr che secand
of tne study.
Blood urea nitrogen:
taboracory Results:
Glucose (fasting):
350 trBldr
1600 qs/dr
Cqrtributor's Diagnosesand Cmrents: Pancreatic islet B-cell degeneracionand
rena
Cdwrts:
Alloxan was adrrinistered subcutaneously at 450 Wlkg and iniEially
produced severe hypoglyceslia due to B-cell degranulation and subseqt"rentlymarked
used
hyperglycemia as B-cells underwent further degeneration. Alloxan was inicially
co study renal toxlcity rntil. Dunr and colleages discovered its drarrntic effects on
islet B-cells. Histologlc effecte qr Ehe islet B-cells include degranulation wichin
Renal
minuEesof dosing folloned by cell shrinkage, nrrclear pyknosis and cytolysis.
changes are predminately in proximal tubular epithelial cells and irnlude sroelling,
pallor of staining, vacuolization, exfoliatiqr and mineralizaLion of cellular debris
within the tubules.
Atterndees' Diapnosee & Corents:
ration and necrosis, islet ce1ls, acute,
mrltifocal, nild to marked, Islets of Langerhans, pancreas, rat. 2) Ilegeneration ard
necrosis, tubular, acuLe, mrltifocal, mild to mderate, proximal cubules, kidney.
Etiologic Diagnosis: Islet cell and renal tubular toxicosis.
Etiology: Coqatible wittr alloxan toxicity.
Crents:
Ttreie rras g€neral agremrt with Ehe contributor's diagnoses ard
cflHlts.
Representative lesiqrs were not present on all of uhe submitted sections
of parnreas and kidney. Lesitrrs ranged frm nild to narked in both org€ns. Lesions
in the pancreas were csrfined to the isleEs and involved frcm l0 to 50% in any given
secti6t.
Renal necrosis was confined to the proximal tubules with mrltifocal mineral
deposits in the proximal cubules and s@ deposits in the distal and colleccing
tubules. tibphrosig wae used to encff?ass the lesiqrs present. A differencial
etiologic agent listed by a fenr attendees was streptoz@n, another diabetogenic and
nephrotoxic cqourd.
Itre trrlo serr.m chemistry findings were discussed: l) Tne
marked fastlng hyperglycemia corresponds well with the lesitrrs i,n the parnreaEic
islets; and 2) Ttre severe azotemia. Itbst participante did not feel the lesions
presenc in the ki&reys a@r.rately explained the elevaEed BUN. Massive protein
catabolism of other tissrrs was proposed as the wrderlying prerenal cause of che
irnreased BUN.. Cautim was expressed that this strain of rat has a high incidence of
applied co
is the term mst cmrly
spqltaneou{r renal disease. Glmrulorephrosis
oy 2
age
becre
severe
to
9
mnths
of
and
first
appear
at
8
chese lesiqrs.
Lesiqts
years of age.
CqtEributor:
KodaE'Tailfi-Eilfl,
Toxicology Sectior, tlealth, Safety & HrmanFactors laboracory,
Eashan Kodak Co., Rochester, I'lY 14650.
References:
frcr,up;T.
C.: Drugs producing diabetes lhloggh danageof the insulin
secreting cblls. Pharmacol.-Rev.22: No. 4, 485-518, 1970.
2. I,Ieaver,D. C. et a1.: Alloxan uptake by isolated rat Islets of Langerhans.
Endocrinology 102: No. 6, U47-l-855, 1978.
r.;aselt - Dog-ilaseL (AITIPL7\7q15).
n Pinscher --anorexia, euesis, obstipa[iq'r for I
weekl-Tx@raEory laparotomy revealed a dark red, flaccid ilewr rvicn dark ;ed
conEencs. An incussusception of Ehe Eerminal iler"rurwas easily reduced. The dog did
not isprove and was euthanized one week lacer.
Contributor's Diagnosis & Comrents: Ileusr.-exhaustionnryosicis. Diffuse
suba
ularis externa, rruscularis urcosa, ard
suhucosal plexus, degeneration of supoch muscle fibers.
Ccrrnent: The lanina propria contains sop mixed inflasmatory cells, but is
relacively wraffected. Nunerouscapillaries in the mrscularis externa are surrouded
by neutrophils. The epiEheliuur is nou involved. Brorm and Brenn stain deurstrated
were negative for organisns. Ihis
Grm positive rods in the luren. PA^Sand @1,S
lesiqr can be seen wiEh obstructions of the intestinal tract, and resulced in a
cwrplete paralytic ileus in this case.
Atterrdees' Diarnosis & Courents:
ion and necrosis, with subacute to cbronic
inflenmation, diffuse, severe, fluscular tLsrics and submrcosa,small intestine,
DobermanPinscher, canine.
Etiologic Diagnosis: Paralytic ileus.
Etiology: Prolonged intestinal obstrucEion.
Cquents: Participants agreed wich the contributor's description of the
lesions. The eciologic diagnosis given by cne contributor was considered tne rcst
plausable in vierp of the changespresent. These lesions were r.rnfamiliar to manyof
those atcending the conference. Differential etiologies listed by participants
included pressure necrosis and ischemia in addition to paralycic ileus. Separacion
of the lasdna propria frmr the epithelial covering of the villi was felt to be a
fixation shrinkage artifact and not part of che subsucosal and nnrscular degenerative
process. VasculiEie and fibrin Ehroubi formation were observed in the small vessels
of the rnrscular ttrnics ard were felt to be secondary to che degenerative changes.
Necrosis of ganglion cells in the subnucosal and uyenteric plexuses was observed by
all and was considered to be a contributing factor in the flaccid scate of che guc aE
necropsy and in the rnrscular degeneratiqr seen microscopically.
Concributor:
Cofr.ffiia-fiEiffie,
Sterling-hlinthrop
Research Inscitute,
Rensselaer, NY L2L44.
- ' ) -
Depc. of Toxicology,
Case III
- 80-902 (3
1757303
Eein calf treated fot 1 week for pnermria and
Laboracory tteeultg: Cranial-ventral portlons of che lrng rere dark red and of
.
ircreacdd-cqrsidEEiffi
Mrltiple snall griy focl rreEescatteied rhrogtnut rbe lurrg.
Cqrtributq'g
Diagnosie & C@ntg:
of both tryphae ad frulctng
Attendeesl
Put-urary aspergillosle.
ypical foi rybtlc
grermrla.
ltrc pres€ltae
'
bodtes are i;lerestirg
flatures'of thlg cage.
lg & Cqrrnrrts:
, pyogrclulmtols,
chrcrlc,
diffuse,
severe,
Etiologic Dtagnooie: I'tycotlc pner.mria.
Etloloey: AsDerelllus ep.
qgre@nt wtth gho cqrtrlbtruor'e diagrnsis.
c-"enEi:
ffi
Participants aloo agreedwiuh tlrC ccrtrlbutorts c@rrte, hmver, fruitirg
bodies were mE observed, A large rn.der of deg€neratirg try'ptraf form snrrorniied by
a radiating eoelmphillc mterial characterized-ar the Sfleifrore- loeppli pherwon
!rer9 P.r?s-eg!ln mst sectlons. ltrcse @y b confusedwith frulrtu€ lridiei. A G['tS
staln lfghffghted rcrphologlq features o-f tte futgur rrtrlch are cbaiacteristic of
fsperetllus sp.; septated and-dlcotpus brarnhing tryphae5-7 nlcrqrg in dlreter.
A cqrurrent or predtspoeingbacterlal infectlm was buspectedin this case by mny
of che attendees.^.Ttre Enall grey foci described at r,ec6psy within the pulmirany
parernbymaqere. likened to those of lur1grcrn.lesime of citile but ttle ybrng age of
the calf mde chie an ulllkely assrmption.
Contributor: llniverelty of Tcuressee,I]epartmentof Pathobiologl, P. O. Bo:(1071,
KnoffIET1ff-19901.
References:
Faffi'lt
_
{ & Rdelg, p. F.: pulmnary aspergillosis in a carf.
JA\IMA
137 (10): 595-596,1960.
2. rlubbr_.f.V.F.,_and_Kermedy,
P.: Pathologtof Dmstic Animle, Academic
Press,
l{ewYork, L970,pp 250-25}.
- 3 -
L4 week old German shepherd dog.
On April
4th Ehe
3rya].19s presented with.voriting and an elevated teryeratrrre. ,this respondedto
Penicillin and Strept@ylin Eherapy. 0n April 9th rhe-dog shored a suddeir
deEerioration. Ttre teryerature wbs elevated, rhe hind lifos and tail shoqedpartial
paresis. The mimal slowly iryroved but never regained full use of its hind li"f's.
One mrth later it was fotnd to be wrable to walk-and hyperaeethetic.
l€bor,a:ory BPsultsi larvae of rhe nenarodeAqgiostroqFf]us cantqEnsis rrere
recovereo frcm EneuEnlrges at necropsy.
Granulmatous reningoencephalmyelicis dr.re
. ^Co?trlbutorjs Diagr.rogisI Gffirts:
EO+-----r
AnglogErons,ylus cantdtensls.
wormAngroscrqrgylus canEonenglsmt'eraEegEo Ene
ixain and then to che pulurary aiEeries
urity.
rn ocirer
mnqnls Ehe migratiqr ie not coryleted srd the sroros remain and cause 'l-qage in the
central nervous syetes.
Attenrdees'
f-renmgoencephalitie,
granulmatous, chronic, mrltifocal,
mderate, cerebelh.m, brain, GeEoansheftrerd dog,-canire.
Etiologlc Diagnoals: Verminouemningoencefualitie.
Etlology:
cantonensiS.
-gngictronsylus
c.nnFnts:
t with the contribucorts diagnosis and
ccrtEnte.
Granulcmatous lesicta were not present in the neuropil of sqe secticts.
l,esiqrs Present in the nemopil were cqtfined to the whice traita of the redulla.
Tbeee and the reningeal lesions were cqlsidered prirqarily vascular in distributiqr.
in t6'e brainsten erere thought.to be a foreign Uoay-reacaion-6-ltre grinul*9
degenerating larvae or larval sheaths. Ttre parasite is a larval- nmatode that has
Polfryyar_iancoelouyarian mrsculaLure and a large intestine coqosed of a fen
mrltlnucleated celle.
In addition tbere are adcessory hypodertal Oords. These
features are characceristlc of a retastrongyle.
Ttre 6izil mrptnlogy of che
incescirre, :rrd the lateral ctrords are conslstent with engiostrbneylils so. Soecific
identificaciqrisnoEPossibleinhistologicsectiq|smiisis
A. cantclensie. A. vasortm, a lungrcro of dog, may resedle this lanra fxrt ic does
fr t-im?IeraTuif$aEiofrffighthedentralnerloussysrem.
CqtEributor:
lturffih-IFiiffiiry,
Division of Veterinary Blology, Sctrool of Veterlnary Studies,
I"turdoch, Western AGtralia6150.
References:
r. Tffi?frik; K. et
41. : Experimentally induced Argiostrongylus cantonensis
infectiqr in dogs. Am.J. ver. Res,3I: [ro. 3, 4494t6--T:97ff
2. 'Rosen,
Mason,K. V. e t a l .: A u str. Vec. J, 52t 205, L976.
3.
L. eE al. : Life history-of _the-canînelungurormA.giosIISZls
vasorrm(Bailtet). A m.J. V e t. R e s. 3l: tto. l, Bf- 143,- 1970.TII'OIHY P. O'NEILL
Regiscry of Vecerinary Pachologl
Deparfrent of Veterinary
- 4 -
Pathologr
Histories
AFIP t.lednesday Slide Conference - No. 19
l8 February l98l
Case I - c2-L4 (AflP L764737).
-&-
an exper.iuencal inoculation
appreciaced.
a young crossbred goat.2L mnths afcer iE received
No clinical abnormalities were
as a young adult.
CaseII - 80-76 (AnP U7$38).
d fror a 9-week-old, iomale, B6D2F1muse, nrtrich
was ffi:l-iiany
in a group that died suddenly. The nice had received 850 R of
ufrole body radiation (cobalt) and intravenous syngeneic bqre marrow cells 7 days
prior to death in a study of sten cell grafc respdre.
Spontaneous
post-irradiacion deaths were expected at L4-2L days, generally fro baccerial
septiceuria. These mice died prematurely at 5-8 days following exposure.
case III
- 0ARB00I(AFIP L773292)
eek-old trEat Eype chicken.
Case IV - 38/tnD (AFIP L727246>.
ear-old quarEerhorsemare having equlne Cushing's
--4diseEse (hyperadrenalcorticism
secondary to a trnrcr of the pars interredia).
TII'OIHY P. O'NEILL
Regisury of Veterinary Pachology
Deparbrenc of Veterinary Pathology
Results
AFIP WednesdaySlide Confererce - No. l9
t8 February 198I
Case I - c2-L4
an exper
appreciated.
Contribrtorrs
inoculation
D
ron a yourq crossbred goat 2I nonths after it received
as a yourg adult.
No clinical
abnormalities were
is a Csrnelrls:
Arthritis
due to the agent of viral
were recognized in brain, joint ard lung tissues
in this group of anirnals which were inoculated Qr the intracerebral
route.
Joint
lesions were noted as early as one week post-infection
ard both continued ard
progressed.
Atterdeest Diagnosis & Cqnnents:
, proliferative, chronic, diffuse, moderate,
joint,
nernbranes,
golt,
crossbred
rynovial
caprine.
Etiologic Diagnosis: caprine arthritis-encepharitis
virus.
Etiologpz: Caprine retrovirus.
Ccrnrentsr Atterdees agreed with the contribrrtorrs et,iologic diagnosis.
Iesions were described as villous proliferation with diffuse iormat,ion of llznphoid
follicles
arrd infiltration
primarily by plasmocytes and ulceration of the sy"i"iaf
memlcrane. The retrovirus also causes lesions in the lurrgs' kidneys, rressel-sard
central nervous system of goats. I?re joint is considered an imnunologically
priviledged_ site ard thus favorable foi viral sequestration. other factors which
may be involved irrlude antigenic drift of surface markers and persistent
infection.
Viral replicat,ion is dependent on RtitAdependent Dt{Apolynerase.
Fersistent infection is due to ircortrnration of viral genffre with host-cell DNA.
The disease occurs strnradicatly in goat herds and is passed horizontally.-Itn Viral
Snrticles can be recovered from the m.mnarygland of infected animals.
caprine
arthritis-ercephalitis
virus is antigenically ard nnrphologically sirnilar to tire
Visna virus of sheep.
Contributor:_ Division of Corparative Medicine, The Johns Hopkins University,
schoo.lT]ffine,
Baltirnore, MD- zI2o5.
Refererces:
I;-ffi;f
C. et aI.: the pathogenesis of viral leukoencephalcnryelitisarthritis of goats. I. Persistent virat infection with progressive pathologic
charqes. Iab. Invest. 42t 596-502, 1980.
2. Cork, L. C. et a1.: Chronic artfrritis in goats caused by a retrovirus.
Scierce 2O7z 997-999, 1980.
3. Cracrford, T. B. et. al.:
The connective tissue component of the caprine
arthr it is-ercephal itis ryrdrcrne. Am. J. Pathol. I00: 443-454, 1980.
Lr.;e II - 80-76 (1\FIp I77t33B) .
tTe,1froma9.week-o1d,fema1e,B6D2FlmouSe'which
oll€ of many in a group that died surlCenly. The mice had received 850 R of
"das
body radiation (cobalt) and intravenous syrgeneic bone marrow cells 7 ,lays
"'rhole
prior to death in a study of slem cell graft response. spontaneous
deaths were expected at L4-2L days, geneially from bacterial
trnst-irradiation
septicemia.
These mice died prernaturely at 5-B diys ioffowini exposure.
Iaboratorv Resulls:
huse
hepatitis virus (MHV)was isolated from a sam-oleof fresh frozen liver
_
frcm this npuse. A polyvalent antiserum for MIIV antigens was used; thus the
subgroup of MHVwas not determined.
Contrihrtor s piagnosis & cormentt
Necrosis, acute, multifocal,
-1:severe,
Iiver, etiology - rnoffi.
Cqtments: Grossly this live! was enlarged with multifocal white spots
throughout.
More typically
the livers of aifected mice were diffusety necrotic,
grossly aptrnared enlarged and uniformly pale and friable.
stress ard inrnunosutrpression_ from exposure to radiation were considered to be precipiiating
factors
resulting in excerbation of latent MIIV infection.
Ttre B6D2FI mice were Ehe only
strain in our institute
which developed the disease despite other strains fron gr
same ard different
su5pliers being used in similar studies.
Serological testirg
(Cr) similarly
irdicated only thii strain to be infected.
connunicition a'rd
quality control data frqn the suplier
shor,vedno evidence of MFIVin their colony;
herce the source of infection was not totally
solved.
The lack of any inflarmatory
cell response was attribr.rted to radio-irrnunoiuppression.
oiffuse fffinoia
depletion also was present in the spleen arxl intestinal
peyer's patches.
Atterdees' Diagnosis I Cornnentss
lbrprorogrc Diagnosis:
Necrosis, hepatocellular,
coagulative, acute,
nn:ltifocal, liver, B6D2FI, mouse.
Etiologic Diagnosis: Viral hepatitis.
Etiology:
Mouse hepatitis virus.
Ccnments: There was unanimous agreernent with the contribr.ttorts diagnosis and
connents. The acute nature of the lesions and ;rast history of irradiatlon
roere
postulated as the cause for the lack of an inflanunatory response. Four strains
of
MllV are knourn. The virus is a coronavirus.
other conaitions assrciated with .VHV
are LIVIM of neonatal mice and deryyelinating lerrcodystrophy
-carriers of mice. The virus is
ubiquitous ard usually silent in colonies oi mice.
are inportant sources
of infection.
Rats also are susceptible to infection with MHV. peisistent viral
shedding occurs from intestinal
mucosa] epithelium of infected animals. Sheddirg
also occurs frqn infected Kupffer cells of the liver.
Hepatocellular necrosis
ffiurs after destruction of Kupffer cells ard subsequent invasion of adjacent
hepatocytes. Nude mice often are used as sentinel infunals for infection in
eolonies.
MJltiple lesions arrd syncytial cells occur in many tissues in nude
mice. Syrcytial cells form in the i.ntestinal tract of weanlirg conventional nice
infected with tqrv. Pseudornonasfree mice are selected for irradiation
studies in
order to eliminate affii-Hse
of post-irradiation
septicemia.
Tire mice are
nxrnitored for this bacteria by using sterile water bottles which are cultured
periodically after use.
Contrihrtor:
ArmeCForces lladiobiololr'
Ua:iafGteil-netiresda,
irtD 20014
Researcir Institute,
Natronal Naval
Refererces:
J. R. et al.:
f-lTEiffi-n'
Lethal enteritis
in infant mice caused by nrouse
hepatitis virus.
tab. Anim. Sci. 262 824, 1976.
2. Carthew, P.3 lethal intestinal
virus of infant mice is npuse heSntitis
virus.
Vet. Rec. 101: 465, L977.
3. Carttew, P.r Peroxidase-Iabeled antibody technique for rapid detcction of
nnEie heptitis
virus in cases of natural outbreaks. J. Infect. Dis. 138:
410-413, 1979.
4. Gledhillr
A. W. et al.:
Ibuse hepatitis virus ard its pathogenic action.
J. Path. Bact. 69s 299-309, 311-320, 1955.
5. HierhoLzer, J. C. et al.:
New strain of npuse hepatitis virus as the cause
of lethal enteritis
in infant mice. Infect. & Imnunity 24: fi8-522, 1979.
6. Ishida, T. et al.:
Pattrology of diarrhea due to mouse hepatitis virus in
the infant npuse. Jap. J. Exp. !hd. 49 (f): 33-41, Lg7g.
7. Feders€rrr J. C. et a1.:
Antigenic relationship
of the feline infectious
peritonitis
virus to coronaviruses of other strncies. Arch. Virol. 58: 45-53, 1978.
8. Tagtrhir F. et al.:
Pathogenesis of npuse hepatitis
virus infection.
I?E
role of nasal epittrelial
cells as a prfunary target of lqr,r-virulerse virus.
}[l1I-S.
t{icrobiol.
Irmrnol. 23 ([lt 249-262, L979.
9. Wardr J. lll. et al. r Naturally occurring hepatitis
virus infection
in tte
nude nrcuse. Iab. Anim. Sci. 27t 372-376t L977.
I0. White, R. J. et a1.: Pathogenesis of m.rrine hepatitis:
Route of infection
of the susceptible host. Am. J. Vet. Res. 252 L236-L240, 1964.
Case III
- 0ARts001(AEIP L7732921.
ek-old meat ty?e chicken.
Contrih.rtorrs
Diagnosis c Ccnnents:
I carcinoma.
Cornents:
Ttrere vras general agreenent with the contributorf s diagrosis.
Skin
was identified
in many sections ry the presence of feather follicles.
the tissue
had been scalded before fixation.
Atterxlees noted muttifocal
epiderrnal ulceration
ad invasion of the epidermis ard dermis by neqlastic
squanous epithelial
cells.
Neoplastic cells fomed cords arxl nests of cells with intercellular
bridges arrt
keratin pearls.
Flitotic figures occasionally were observed. Ttre origin of these
reoplastic
cells was Srostulated to be the feather follicle.
Invasion arrl
destrtrction of skeletal muscle occurred arourd feather follicles.
TtE irciderne of
this lesion is about 2/L0r000 in broiler chickens at slaughter.
Metatastasis is
mt retrnrted.
Ttrese neoplasnrs are leally
invasive, may be sirgle or mult,iple ard
occur on any part of the body. A seasonal irciderce
in the spring is associated
with this lesion.
The cause of these neoplasns has not been determined.
Contribrtor:
Bureau of Veterinary
eertE;IlId;E-2ozos.
ltbdicine,
Food & Drug Afrninistration,
Reference: Turnquest, Robert U.: Dermal squaflpus cell
chickens.
Am. J. Vet. Res. 40 (11): 1628-f533, L979.
- 3 -
carcinqna in yourg
Case IV -
:
AFIP L727246
I'rssue trorn an
r-old quarterhorse mare havirg equine Cushing's
radrenalcorticism
secordary to a tunrr of the trnrs internedia).
contrihrtor's
Diagnosis o conmentsr
schizogenous forms of a coceidia
(probably
.t"ifr.iial
cells of rhe pirnal glarrt.
sunuar structure?
present
in
vessels
in
the
brain
ard renal-glonerrlli.
lere
Nunerouscoeidial.forms
were present in the renal tubules. There v*asno elrurar
req)ons€ to these intravascular organisrns nor crinical signs
of tireir pr"""rr..
Attee{eesr Diagnosis a Connents:
aiial cell trnrasitisn, multifocal, mild to
.
nrcderater pineal glard, horse.
ELiologic Diagnosis: parasit,ic vasculoSnthy.
Etiologlz: protozoan, possibry
srossieria gi. or.F?rcoqrst.is gp.
ccrmentsr An etiqncrphologic-aiagnoffi:f
parasiffiropattry
was preferred
by nost att'enlees. A tenuous issocialion-uei*"
erdothelial cerr [nrasites in the
pineal glard ard kidney tubr,rres
u"tw p"rticipants list
ry9 lrayr by participants.
etiologi"
eP. as difierential
Differentiation
*.cq?yPtis
between
Krossiella ard lqqcggYstis courd not bi mad6.
"i;4". Electron photoruicrographs
of the
parasi:EFFroviaEl'@ffioniiiurtor
aenpnsiiared coe idian ;nras
ires
within
'-,rp
erdothelial cells.
Parasites were 1.5 x 6 microns in size.
are present within parasitophorus vacuoles in enlotheliat cell3 ao 50 nerozoites
of the;il;l-grard' Also ccmnentedon was the obvious lack of
host respo*. to the preserce of
the trnrasites. The retrnrted cushing's dis-ise conaition-nivGve
contrihrted to
tttis inmunosuppression. The life {cres oi u"ttt sarcocrsiii
aid
-s;ffi"I'e
rtossietla
rrrere
revier*ed. Three erxlotheliar replicitive
gtases rccu:-fiEvin
t'hird replication also occurs in circulating-biood cells hrt
is considered a minor
conqnnent in the life cycle. The life cycre of sarcocrtstis-in-trre
equine has not
been fully elucidated.
ltre possible rorl or sirEG?stE.
protozoal
in
Equine
E r c e p h a 1 o r n ye l i ti sa l so w a sd i scussed.The"nffir d"ofxr ossieI1aeqtr iis
no t k n o u r t . A sch i zo n tg e n e ra ti o n developsintheer dot' heliut".iiffi,,ffi
capsule' t'lerozoites pass d*nn
convoluted tubules with the renal
!g the pr6xirnal
fluids ard oenelrate Lhe epitheriar
..iG.
rere _they deverop a secord generation
of schizonts' The merozoiles fronr.these rupture from the hostrcerl
into the renar
fluid'
At this point, these merozoite" .i*rii
pss outward or distally to the loop
of Henle where they penetrate the epitheriar ceus and unoerto-garnete
formation.
-sp.;".ry;|"
spororysts are formed from these garetes ard are passed in tfie
uii".
are ingested bv the host, white giazing, arxl exqfst in the Jig""ti".
tract.
sporozoites presumabrypass through thi.intesti;r
warr, entei the blood,
eventually reach the gJ.omeruli, enter the endothelial
the crycie is
retrnated.
""fi=r-*,a
Contributort..Fpaftnent of Pathology, School of Veterinary t6dicine,
univc@FG-rifornia,
Davis, cA 9561c.
- 4 -
References:
ffi;ll.D.etal.:TheCoccidianParasiLes(Protozoa,Sporozoa)of
Rodents. The univ. of rrrinois press, urbana, Lg65, pp rgo-ras, 342-345.
2. Taylor, Jack L. et al:
Klossiella parasites oi anlrnals: A literature review,
vet. Parasitol. 5: 137-144, Lg7g.
3. Todd' K. R- et al.:
Kloss.iella equi Baumann, Lg46 (Sporozoa: Eucoccidiorida)
from an rtlinois
horse. vetJGffii'Eilim-crin
72: 443-448, Li77.
4. Vetterring, J. M. et al.:
Klossielra equi gaunann, Lg46 (sporozoa:
Eucoccidia: Meleina) frcnr eqr:ids. .rlarasiEff
5g: 599_594, Lg7z.
TIIlt7Nry P. OINEILL
Captain, USAF, BSC(\C)
Registry of Veterinary pathology
DepartmenUof Veterinary pathologry
- ) -
Hiscories
AFIP l,Mtesday Slide Conference - No. 20
25 FebruarY 1981
1716398
colt had mrltiple subcutmeousn{u19r Passq in ttp
TtE
Uiopsv tndiLarcd poesible lFPhatlc haartoa.
l".f-l--fniitai
U-,:o -_
letl IIE.to
rear leB:
left 1f"
infittrating try 1"!!
Lelan infittrating
and [relan
enlarged and
siiefy enlarged
F;gi"ssiiefy
CaseI-#1-
ilil
aliiicuirfot
copi'"d rGEicat exciEiona por p:ognoslsmg given. Ttrecort was
ldentified at
wae identified
naes was
and
retrooeriioneai mes
and retroperiioneai
;;;;;;#J-;;l;-aMoriilar
il;il;i;J-;d6-audorifoI
p""6;;t"t.
rtt" t""; -*?" ;*"t-tt
to cire lef t, tq*:
y::ykg:l
left
tbe ?:*iodorsal
Ehenia-je:unm, the left testicle and:*
--l"iifi""iiu"-oucgioryths.traieised.
fiffi-Fitt.'pJrui"-iniir,
extended
exte
and
canal
canl
inguinal
inguinal
prg.
ile
tF
d+ised
riiiriiiii;"-il;#orrh"
kiffii.
]?f!,
i"io ttr" deep inrernrgcul[r faecia of the left hindltd.
laboracorY Reeults:
gaetrointesclnal o,lcosa and congeeted liver.
ffiddened
tibcropsy rev
oryo""iafut ilae pale wlth enall wtrite subendocardtal sPots.
CaseIII - 80-15072(AFIPU584!3L
cattle
farm.
The
bovine fetus srttictrwas found in tne field of
i
cqserv - AN80/254.sH80/trt0 (Rrlp tzetqse.
riF6ffi
cattle 296 of which had been grazing
AN 80/254 - A mortallty lnvol.ved a grouP o! !lobeen inrroltuced ro rhe properry aad
kikuyu pasture tit 2 wleks and 1g0 Jt nnirr"n had
had not prevtouslY grazed klkuYu'
and
had been heavily fertillzed
Ttre cattle qrere l.ntroduced to a p.ddock whlsn
feet
2
over
feed
an overgrowth of
irrtgated over the prevtoue 2 rnonthe and contained
hlgh.
Day3-ldead
;;i 4 - 5 dead, all
Day5-19dead
Day6-10dead
DayT-2dead
DayS-1dead
cattle
removed from paddock
days afrer acceas ro lueh ktkuyu pasture'
sN g0/r310 - stmrrar hrstory of loegee 4-7
dehydratlon, wlth depreselon'
slgns lncluded exceasive sallvatlon and
cllntcal
weaLnees, recumbEncyand death wtthln 24-361lrbure'
and carcases Were markedly dllrydrated'
At auBopsy mucous nernbraneeltere cyanotlc
henorrhage' Orere were ueually
There wag coneletently agonal ecchygotlt eplcardlal
waa
n the abomasumand snall I'nteetlne there
no grot, leslone tn tire fO""stor"""tr.
ptl
of
The
and ktdney were congeeted'
occaetontlly patchy nucoael congeetlon. Llver
runlnal cont€ntg ln 2 aninale waE 8 and 9'
I.ABOMTORY RESULTS:
(3 anLnale)
Sanole 3
L 1. 1
Calclurn rrgldL
Sapple 1
9.9
!.lagneelun ng/dl
3.1
3.8
2.9
8.1
9.7
8.8
3,7
4.3
3.9
112
88
108
52'2
41.0
50.3
42L
L32
149
TotaL Proteln
Albuntn
g/dl.
g/dl,
Urea mg/rnl
Urea Nltrogen
Glucoee mg/dl
ug/mL
@
L2.8
P. OINEILL
TIM OTHY
, sC(Vc)
C a p t . , U S A FB
Registry of VeterinarY PathPJogY
Defartmlnt of Veterinary Pathology
Results
25 Feoruary l98l
CaseI - #f - (efrP L7L6398
colchadmulciplesubcutaneousnoduIarrnessesinche
.
F--+left inguinal region. Initial biopsy indicated possible lyqhatic hanerE@a. The
massesprogressively enlarged and began infiltrating
the lefc rear leg. Dr.reco
difficulty
of cmrplete surgical excision a poor prognosis was given. The colt was
euthanatized and an aMmrinal and recroperitoneal mass was identified at
posEprtem. The masswas adherent to the left lunbar musculature, the craniodorsal
aspect of che pelvic inleE, the mid jejtrnLm, che left testicle and che lefc
kidney. Infiltrative
outgro!'rths traversed che left inguinal canal and extended
inco che deep intermuscular fascia of the lefc hindlitrb.
ConEributorrs Diagnosis: Cystic lyuphangicma.
Attendeesr Di
c .[yuphangimn, horse.
Conents:
Attendees agreed with the contributor's diagnosis. Chronic
uurltifocal cellulitis
also was present.
No organisms lrere observed in chese foci.
cystic spaces lined by endothelial cells and surrounded by dense
Lyrph filled
connective Eissue ccrprised the majority of the neoplasm. I'titotic figures were
rare.
Loose slightly uryxooultousareas also were present along wich dense areas of
connective tissue.
The large and invasive nature is consistent with that described
for cyscic lyurphangimra in man. Several participancs eonsidered lynphangiosarccma
as a differential
diagnosis but histologic criteria
for malignancy rrere lackirg.
l4any felt that this Eunor was congenical in origin, arising fror eurbryonic rescs of
These turDrs are considered
vascular Eissue, with subsequenEgrcwth after birth.
rare in animals.
Hemangicmasand henangiosarcsns occasionally are observed in
young animals.
Lyurphangimas are placed into Ehree categories in man: l) SiryIe
frm lyrylrangeccasis;
lynphangiuns, which are hard to differenciate
or capillary
2) Cavernous lyrphangimras, r*rich are characterized by thin walled dilaced Ly4h
vessels surrounded by variable arpurEs of fibrous connective Eissue; and 3) CysEic
vessel-s
lyrphangiornas or hygroras, wtrich are large dilated and cystic l1ryhatic
suriouriled by thick fibrous connective Eissue. Normal tissue structures may be
interposed between cysEic spaces in this last category as well as grohtth along
fascial planes. The inf lanracory foci observed in tiris case was felt co be
secondary Eo the surgical incervention, and not a part of the tupr.
Concributor:
Dept. of Veterinary
Pathology & l4icrobiology,
Washingcon Stace
Unive:Ti$l-FEl[nan, [^lA 99f63.
Reference: Turk, J. M. et al':
L228:viurer9.
cystic
in a colt'
ly'u'phangicma
JAWA 174:
Case II - 79P2'/40(AFIP r7573rf) -
Labradorretrieverpresenteddeadafterbeing
ristffiLor2daysand4lCtemperature74nourSpriortodeach.
Laboratory Resulrs:
gastroincescinal mucosa and congesced liver'
Necropsy r.u?ff*TT"ddened
myocarditrn was pale wifh sunll whiEe subendocardial sPots.
The
ContribuCor's Diagnosis & Conmencs: llyocarditis due Co Trypangsorn cru?L'
uch Louisiana and hailnot been ouEsicle che
was
The"nunber of arrascigoces was excessive. Identification
area since birth.
nradeusing light and electron microscopy.
Atcendees' Diagnosis & ConuPnts:
is, granulounEous, diffuse, chronic, severe,
i
retriever, canine.
-niigros
Labrador
heart,
"---iiioirii"
lrypanosouriasis (protozoal myocardiE is ) .
is t'Myocardial
TrYPanosora crYiEtiology:
diagrrosis.
There was unanimdfis agreement with the concributor's
Cssents:
and large
resPonse,
inflarmatory
tissue,
was based on th;
Att""alu";-di"gloris
fibers.
nyocardial
within
nr.-rnbersof cryfanosoural amastigoces
nucler-s
The amastigoEes measured 2-5 microns in dimreter and concained a large
che
of
differentiation
Ehe
in
A courent was made
and basophilic"kinecoplast.
o.f
of che
ciosery related members
.An'nstigoteq
"8r9uP:
thus are not
and
system
lefskrmania spp. prr""icize cells of the reticuloendotfrelial
il;;i;;'i;&-"ih;i
?ffi""Eirui"iinmyocardialfibers.Trypanoscmarhodesiense,T.congelense,
a n d T . v i v a x ( i . e . , c a u s a t i v e a g e n c so f A f r i c a n
il'"iutt6ffi"iigoc6
stage in rnEii life cycle. uai,marsare most cormrcnlyinfected
ics
with T. cruzi uf-one of"several modes, I) Ingestion of the triatonid bug or
crre
of
feces
the
with
of wounds, bites or unlcousiircrnbranes
;;;;i'Zffir"rinarion
be
also
bus,: or 3) Ingestir:n of tissues oi inf""ced aninrals. Transmission,may
oPossuns'
Efooa Lransfusions. Ntrerous wild aninnls particularly
."8*rpfi"fr.a
organism'
Ehe
and armadillos in fire Southern U. S. harbor
i"""oot",
Dept. of Veterinary Pathology, School of Veterinary l'tedicine,
Contributor:
Baton Rouge, L,A 70803.
t ouiGfZiffi-universicy,
References:
T. ,A:n'seTfie, A. et al. : Pathogenic nechanisns in Chagas' cardimtyopaEhY.
Disease
In Trypanosouriasis and Leishrnaniasis with SPecial Reference to
fS,
(
n
e
h
,
s
e
r
t
e
s
)
,
un ZU
Ciba Foundacion
123-136.
pp
1974,
Amsterdam, London, NewYork,
llYocar ditis caused by Trypanosomacruzi in a
2 . S n i d e r , T . G. III , e t e l . : '247
-'249, f980.
native Louisiana dog. JAVMAL77:
'trypanosomiasis(Chagas
Naturally occurring
3 . W i l l i a m s , G."D., eE al.:
l-71: L7L-L77, L977D i s e a s e ) i n d o g s . JAVf'tA
- t
case III - 80-15072(AFIPU58403)
--i
ff
cattle
.. 1 ,
bovine fetus which rvas iound in Ehe f ield of
farm.
Laboratory Resulrs: Brucella abortus was cultured frorn gascric conEenEs'
atteslpts for IBR and BVD were
anffiirus-sofacion
spleen, ano rungT*E-TesF
negative.
.
---r-#-
Contribucor's Diagnosis & Couments: IbningiEis, suppurative, nullifrcal,
Lla aborcus'
mark
]'licroscopicfindings.
Cmments: Gross examination rGGl-no-dftnfficanc
leptmreniry"l:--I!!,
in
the
weie
affy, or"if."a mulcifocal cellular infilurates
^,
varied frmr one area to anocher and, in ncsc areas' were ccmPoseoor
infiitraces
Coccobacillus bacteria were observed in macro-phages...The
cells.
mlvrprphonuclear
Extracerebral lesions included marKecl
h",-tiopri"nchyrnl change !{as very limiEed.
joiqc,
mulcifocal accr.rsulation of rcnonuclear
mild
FiUriiropurulLnc exudaEes in thi
in the porral cracts of che liver.
and
the"kidney
cells iir tfre inteiscitium of
findings in bovine fetuses aborted due
untmuron
are
not
rrrd encephalitis
G"i"giii"
to Brucella infection.
is & Cmments:
is, granulomatous, multifocal
n
bovine.
fetus,
brain,
meninges,
upderate,
Etiologic Diagnosis: Bacterial meningitis.
Actendees' Di
Eo diffuse,
eciolo[y, BJicer]agp. (probabry.s.
?F5!Y:).
meningicis wich
pyo-granulomatous
Ccrsencs: The rnaJorrry or partili[ants ffied
causedby
abortion)
novine Abortion ifoothiff
*re eciologic di;g;ri.-oe'Enzobric
diagnoses-given
Ciir"*yai"l"organi5ms as their preconferencechoice. Differencial
The cellular responsewas predoninancly large
were Brucellosis and Listeriosis.
and small nrcnonuclearcells with only a few neucrophils. Degeneracinghistiocyces
--Sr* cells and resulted in misinterpretation of Che
*ioti"t"a pofyrmpfronuclear
trisriocytic cells conEainedsmall.Pale scaining
inf tanmarbry'r""fioil.
bacilli rhar could be seen in rhe H&h sained section. The majoriry of che
paiticipanrs were unfamiliar wich this feEal lesion of Brucella aborcion.
Classically a granulornatouspnernonia is the lesion trDst ccErlrcnlyreported'
and hepatic^lesions also are reporEed.
ffiilG;,'r"nil
Contributor: Livestock Disease Diagnostic Center, University of Kentr"rcky,
fa29r.trrewffi'-Tl[-e, Lexington, KY 405tI Refefences:
ffi.UlterioriRicercheSullaNeuropatologia,DeiFetiBovini
Aborriri, (Further research on the,neuropathology-offhe aborted feEuses)'
annafi Fac. tted. Vet. Torino. 24 56, L9l7'
Tierheilk'
2. Guarda,F.: ProblerniAttuali Degli Aborti Bovini. schweiz.Arch'
LZL 3?,3-326,1.979.
J
-
case IV - AN 80/254r-!ry
1r1o (AFIP17614q92 s l i d e s .
had beea grazing
and
296 of which
cattle
involved a grouP of 476
mortality
A
t o the ProPerrY
80/254
AN
180 of which had b e e n i n t r o d u c e d
krkuirs Pasture ;;;-'-;;"ks.
lld
grazed kikuyu'
had not'previously
The cattle
o*'"t
irrigated
high.
to a paddock which
were introduced
2 monchs and contained
tiJ-frtt'iot'"
Day3-ldead
Day 4 - 5 dead,
D ay 5 -19dead
D av 6 -10dead
all
cattle
had been heavily
an overgrotrtth of
fertilized
feed over
and
2 feet
r#-a*ôAdnck
removed from paddock
nayZ-2dead
DayS-1dead
SN80/1310-Simitarhistoryoflosses4-TdaysafteraccesstolushkikuyuPasture.
dehydration' wiEh degression'
excessive salivation and
i
n
c
l
u
d
e
d
s
i
g
n
s
clinical
hours'
t"a deat'h within 24-36
weakness, t"""*iEitv
A t a u E o P s y m u c o u s m e m b r a n e s w e r e c y a n o t i c a n d c a r c a s e s w e r e r nThere
a r k e d lwere
y d b t rusually
ydrat'ed.
agonar
there was
i
n
t
e
s
t
i
n
e
s
m
a
l
L
"pi""tliir-t"*orrhage'
a
n
d
there was consistently
"""iyrrrri" I n t h e ; ; ; ; t ;
iot""toi"l'tt''
T'hepll of
no gross. lesions-in t,,le
and kidney were congested'
m
u
c
o
s
a
l
p
a
t
c
h
y
^Liver
occasionalty
" o was
" g " " i o8nand
'
9'
ruminal .or,a"ot" in 2 aniroal"s
(3 aninals)
Spmple I
o
Calcium mgldt
Magnesiuro mg/dL
Total Protein
Albunin
g/d]-
g/dL
Urea rng/rnl
Urea Nitrogen iJ3/rnl
Glucose mgidL
q
Sample 2
L2.8
3.8
ggrtple 3
1 1
1
2.9
" ' l
8.1
Y " l
8.8
4.3
3.9
]-Lz
88
108
52,2
41.0
50.3
L32
L49
5 . t
42L
- 4 -
dif fuse) '
Contribucor's Diagnoses& Conments: 0rnsitis, reciculitiq, (u1'gtty
infiltration'
irosis wich neucrophil
n
"r.
Kikuyu-poisoning'
nephrosis.
cortical
A"utu pttcfty
acidity
conrrenrs: Marcinovich and Smilh Cig73) nocudl" decrease in aborasal
range
normal
Ene
tn"L rr.minal pH was within
and, in contrasE to grain
poisonirg
"ngotg*"ttC,
beti^reenso-calred Kikuyu
in caEEle wirh Kikuyfi-p"iro"T"g::T;"'similarity
noced (Marrinovich et al' L97'2)'
b"un
n"s
mfcoto*i.or""
of catEle and 2 explriiental
AEcendees'Diagnoses& Counents:
it, cubular, acuEe,rnultifocal' mild co
o.paeraCb,tiEney. 7) Nu"ro"is, epichelial, larellar, acute, nulUifocal, trlast'ut,
rurrEnand reEiculun, bovine.
Etiotogic Diagnosis: Toxic nephrosis,.omasitis, reticulicis and ruuenicis'
fifuyu grass cox,icity (Pennisetunctq44eqeinun)-.,
iriofo[y,
'?
4iagnoses and
Ccunencs, Ttt;;6 *;s-generat-agieeme
by
a
differential-diagnosis
as
often
ccrrrrEnrs. urea p"riling"was listEd rpst
hyperglf-i::.,::9
alkalosis,-a
r,rlfl
a slight
Rlcendees"noced
priri"ip"r,t".
*.
lrignrry elevared blood urea level in the clinical pathology,T:ul!:^P::::?::rol Eo
che"contrioutor. Attendees agreed that the histological lesions veerecontrneo
oi rhe'-mucosa.Ulceration w6s considered artifactual
;i;; ;;p";ii"i"i-ray"is
and herorrhage in the laslina propria did noc
n""rosis
because inflaurnaii6r,,
this. Renal lesions were confinel to the cortex, ind consisted of
rnterstitial nepbricis nas
"""*p.ny
degene""ii*r of rubular epithelial"cells.
;;dia'and
also been reporEedwith this toxicosis. e_sitgle toxic factor has not been
faccors
isolated in this disease. At least Ewoand poJsibly three interdependenc
of
presence
2)
The
exisr: l) The fiitry, iiass which-rffrycontaih a coxic.Plinc-iple;
elaboraced
SpodopteJaeJ(empta;and-3) l'tyiotoxins
;;;-;r#
i;rt-;;;"pTiruirl,
Ehe g-rass. Studies have shor,'mEhat
iicrc::arl:rna.r
rne
giooo'in
ritric[r
ir"gi
ir*t
in"-i*gus or Ehe arrry w5rus reduces the indidence of che
or
"iEit"i
"li*in"cTon A few attendees cqmented on the similaricy of lesions Present in Ehis
ai""r"..
case to Ehoseobserved in sweating-ri.r.""i" in calves reported in'South Africa.
Contribucor: RegionalVeterinary Laboracory,Wollongbar,N.S.W., Auscralia'
References:
f f i . 0 . e t a l . : A c u t e r r . r m i n a l i n d i g e s t i o l l " } X 9 ^19,
r o sIvbv.
isanddeathoi
carEle grazing Kikuyu grass. New Zealand vet. J. Ll :
Acute i,"tinaf indigescion, alkalosis and death of caccle
2. Busch, J. eE'all:
grazing Kikuyu. New Zealand Vet. J. L7: L82, L959'
between so-called Kikuyy-P"i:9ling of
iimitariries
3. tr4arcinovicrr, o. er al.:
rrycoCoxicoses. New Zealand Vet' J ' 202 57 ' L972'
cattle and two
1. Clinical &
"*p"tio*tCal
fituyu poisoning-of cattle.
4. t4artinoui"f,,-0.-"t
"t.i
L973
55,
2L:
J'
Vet.
New.Zealand
pattroiogi."l fittdittg"Kikuyu poisoning of caCcle' 2' Epizootiological aspecEs'
5. Smirh, B. eE al.:
N e wZ e a l a n d V e t . J . 2 l : 8 5 , L 9 7 3 Kikuyu poisoning of cattle in WesEernAustralia'
6. Gabeedy,B. J. eE ai.:
A u s t r . V e E .J . 5 0 : 3 6 9 , L 9 7 4 .
Kikuyu-grass poisoning of catEle in Nacal' J' Souch
7. Bryson, n. W. ei al.:
African Vet. Assoc. 49 (f); L9'2L, L978
An outbreak of "Kikuyg n9iggning" in wesEern
8. Van Ueerden, -1. *r-ut.:
1978.
T r a n s v a a l . J . S < l u l t rA f r i c a n V e t . A s s o c . 4 9 ( f ) : 2 7 - 3 A ,
TI'VOil{YP. 0'r\[ILL
Captain, USAF' tlSC(VC)
Registry of Veter inary Pattlolo$/
Defurrnr:nc of Vecerinary PachoJ-ogy
- 5 -
Histories
AFIP WednesdaySlide Conference - No. 2I
l8 l'larch 1981
Case 1 - 8UA4
P 1757061
Golden retriever
of
tlrsL(J|-y vr
wrtn
with aa history
was presgnEeq
Presented
'and
showed lareness in che
righE !iPl"
lesions in tbe righc
lytic lesionsleg. X-ray revealed $tic
me leg.
-.rE"f"r- of me
!iPl"'
of mrscles
atiopny
.i?dpni-"i
che ri$hE
below
fiscula
a
driining
reEaEarsuS.tne 6o9 had
and reEaEarsus.
rarsus and
fibula. tarsus
iiilU;
The dog died and tissues $rere ttken at necroPsy exaninatim.
ilk-
Case II - 1889 (AFIP L7M7Y)'
t (Charles River, CD); control animal; ryale; birch
dare5lTl$-; sacrifiEed Lll4l80; sac weight 600 g.; sacrificed becauseof inappeten@
and general unthrifty appearance.
LaboratorY Resulcs:
m00
.
CaseIII
-
r4.5
HGB
ICV &.5
ttcT 42
wBc 5490
Plasma Protein
- 80-0356 (AFIP L757M8).
with history
manifesced bY rubbing' scratching,
woolTlffiis
snncking and nibbling whenscratched.
5.8
of weight loss, pacchy Loss of
nibbling fleece; rnild ataxia, liP
CaseIV - 9-208-80 (AFIP U58839).
Pygrrrcffi
2 miciosliies
abortions occurred in a research herd of African
(H&Eand Giessa) included'
'TI}OftT{ P. O'NEILL
Relistry of VeterinarY PacsologY
Deparcuentof VeEerinary Pachology
ResuLcs
Slide Conference - No' 7L
AFIP Wednesday
-18
March l98l
G o l d e n r e t r i e v e r t ^ , a s P r e s e n t e d w i E h a n i s t o r y oche
f
lameness in
'-r \ 'tys
showed
weak,'sd
anoreccic,
illness ot tU cl€ duration. -rt-td""
rigt,L hock to abouc 6X9 cm' and
righc rear leg. , There was enlargo*rrc of Che
lesions in uhe righc Eibia'
a.rophy or muscres of me leg. i;;;-r;u""i.o-ricic
f istula.below che right
fibura, Earsus and merararsus. Td-ad-;.J-.-atirining
hock.ThedogdiedandtissueswerecakenatnecroPsyexarninarion.
Cqrcributor's
is&
Di
ewasagr
CoccidioidotYcosis.
il;""ty;iiiil-"lr"cting
the cibial f-i?:y
t;;-;il!-""rr"riiis-of,91e.:o::^.i::y"?^?::',lli,?i"*
'lfrJ:'ffi"'!ii*'I"4;lyis::,:^:n:i:i:"'li*,:T:,i"i:!,lli"li'.
*d ;ffi3i"in.'iiei.-i",i
3:i"lT:.::
l"i,fis:i;ilil.i;-G*gqg lgig, :"::":::?:i":::il:
X?".f,
iffi:"3:'3nn"ili5l
NUUETOUS SPtlsf ulss
lesrutrr'.
bone
DOne lesioirs.
(rr
Lrrs rsrSsr
were focil
There
r
----;-
areas of granul*tt:oY:-q5gll:^-^-
r presenE
aLSO,
,{rainino
sice of initial iniectior,. rnitial eresg{:!t:: :l^":::*f}t::t.::"*iitll5"
o:g:,"T:.
i .'
ii:il:" T li: i J:iii'H";.""""'iJ "t;P:; ::-:l::f;"
of "t
*.:"0;:::"::ff
because
resions,
ii?5"iffi*i:"Hifi::xf,ii|H"ijE.;;J;;;;"i"*i"r.'".i"e-'["r,
hazard c5 laboratory Personnel'
is&
itis, pyogranulomatous,chronic, diffuse'
bon"-*rrowl righc hindlimb, canine'
-- i t is .
""u*,'
;; i.ioet" oi"s,oq ! r! - c"gc idioidomycoEic osteomyel
niioroEY' Co6cidioides
l$cit'
wiCn tne concriDucor's etiologic
CdtrEnus: There was unanl*dgr"gTeqt
t*t of t'ihich contained many
diagnosis. nff observed large chick"walled-spheiuiut organisms were also
iy,,,,,,,,,,,,,,,9cured
endospores. rc.urio""l douoie ""tto"t"J-and neutrophilic response wich
intense
an
incited
observed. Rupcui"a-ioro,"
disease was brief Iv reviewed'
microabscessformation. f6e patf,og;"ti" -of chis
formandu:i*-:i:P="'
of c.-iqmiti" a"t""'iT""iiy"ett";;-;6h;l in a granulomatous
Archrospores
pnewpnla'
host andresurE
Thearrhro"por""?ffi"J-uy-1r,"
endospores'
of
devEropmenc
wicn
These arrhrospor"!-in-tui"-J"u6rop-ini;-"Ph;rules
limiced in inosc individuals wich
,Thepuraonary-fonnoi rn" ais""sJi;";;";iit-s_eri
resr,ns. Radir.graphicalry
desErucEionot che organr"o," *i*ruroiuii"rtot theirte
r'rith U!9 quhmary fonn'
dog
and
so-called "coin" lesions oru ,..r,-i;-;"
disease
the,pnerioni'1:
-?]::Sinated
Local lynph node involvement t*y't"q*p-tny
ff'u endospores.Eovarious Cissues of tne
resulcs from the hematogenou"
"pru"J-oh
infection is reported in che
uo.,"i--Gni;g;;t
oody msc ,,orruiy"&i;-ilo
and
ffi:#;;.*i"ii*""se
a-neciiastinal
in hr.rurns
cil.'- in"iicct-e andhorses
and may be confused wich
a
oUseived
cracheobronchisl lymphacleniri, il'"*uli*"
Cell-rediated inmunity is necessary
Cuberculous lesion at necropsy oi sfaugnCer'
antibody. to the organism is noE
Circufiting
for recovery frr-rn the diseaie.
sollble substances released by the
protective and uuly alone or in .oJ*"Ciot'"*iff'
(2)'
Alveolar ancrophages
i*tnicy
fungal organisms, suppress the ;;ii;;i"i"a
descroving C' imticis
oi
U"-in"lp"iu
c"
alone, from rhesus fi)nKeys n"u"-uo.";h"tt"
'ugBt'l-l .conple't*nc deticienEyTALso recenE studie,
organisms in vicro (l).
in inf eCCedind iv iilu , ts (1) '
boch cla:;s ica L ,nd alcern,rf ive ;;;il"t;
Concributor: tos Ang,elesCouncyDept. of Health Services, CourparativeMedical
& VeEeTfia:]-ffivices,
L2824Erickson Ave. , Downey,CA 90242.
References:
@L.ecal.:InvicroresPonseofalveolaruncrophagestoinfeccion
with Coccidioides iumitis. Inf . & Imrnrn.282, 594-600, 1980.
2@
Affilmonary
coccidioido'nycosis. Med. Clinics of North
America U+z 46L-473, 1980.
3. Coccidioidurycosis. Chapt. 17, l"bdical Mycology, pp 230-253.
4.Ga1giani,J.N.eta1.:.coupr6@btrccidioidesimitis:In
vicro and dlinical. studies. Inf . &'hmun. 282 944-949,-198F-
River, CD); control animal; male; birth
600 g. ; sacrificed becar.seof inappecence
LaboraEorvResults:
ffi00
I\CV &.5
rcT
L4.5
HGB
PlasmaProtein
5.8
42
I^lBc 5490
Contributor's
Djegpgsis & Cquents:
lyrphosarcoma.
Histiocytic
op of che head and swelling exEended dorm
the dorsal surface of the neck and showed signs of erosion through che skull.
Additionally
there was fluid between the brain and che roof of the skull.
There
were raised nodules on the lung and on the left lobe of the liver which were
histologically
similar to the mass on che head.
Attendees' Diasrosis
& Couments:
subcucis and bone,
cdEl, hisciocytic,
Sprague-Dawley rat.
Corrent:
There vras a wide variety of preconference diagnoses suhicced by cbe
participants for this neoplasm. Poorly differenEiated or r"rndifferenEiatd sarcoma
was trDst often listed.
The final nnrphologic diagmsis was reached after soe
discussion.
was
The turnr
characEerized as an expansile solid mass which in soue
sections invaded mrscle and bone. Cellular rorphology remained fairly uniform
consisting of large plemnrphic histiocytic
cells with abundanc eosinophilic
cytoplasm. ltrltinucleated gianE cells were observed frequently.
The mitocic race
varied from I to 3 per high power field.
SErmra and accoupanying vasculaEure were
wich
sparce. Tlris neoplasm was cqrsidered unconnpn in the raE by those faniliar
rodenE rreoplasms.
Contribucor: Environrpntal Proteccion Agency,I'dail Drop 72, ResearchTriangle
Parklffi/ZfF
'
,
i
- 2 ^
l
Ca s eI I I - 8 0 - 0 3 5 6(A F IPU 5 7 !{9 L
HlSEOry:
J-
;:-'-'
rrcol, prurlEls tr;;;"ri"a"[y-r"iui"i,
srnacking and nibbling when scratched.
- - !- 1.- 1^^^ .- i
wich nistor y of wgignc- Ioss'Pal9ly loss o r
scratctring,nibbliilg fleece; mild aEaxia, Iip
AEtendees' Diagnosis & Cmrencs:
vacuolization, multiple' brain scem nuclei'
and gray matEer spinal cord, Suffolk, sheep,-ovine'
Eti6logic Diaigrosis: Viral egcep!1|ogfelopathy.
Sciapie virus (unclassified virus) '
itiofo[y,
rorptrotogic'diagnoses were suhritced bv-rbe
6;;;i;t
c*r=ncs,
;;;;"i;;il;
conference attendees ini:luding spongiionir encEphalorfelopachy, spongiforP qoli* viral
Eti6logic diagnoses incruded
u""*i"il"g"".uropachy.,
;;;$;i"ny"rop.rr,y-"td
viroid encefhatouryifopbchy,scraeie ang viral
alg."li;ion,
il;ffi1
enceohalouryeliciJ. Vicuolated neurons were preslnt in Lhe spinal cord and brain
axon sheartrs containing eirlarged axons weie considered co be
;;;:"-,il;"Iioii""
Ttre hiscory-coyprq{ wich.che
processingfarcifadts.
inaloi--ri*"ii*
;;"-;;
bf chb diagnosis of
""a
juss€stive
in rhi neurons"i"
;;;l;;";-io*a
fi;;J;;f"'
"trongll
also include
should
Elssue
cns
ru"pecrca scrapie.infected
;;;;pi;:--eu.iuriio-oi
ascrocvtic
of
and
prolifeiation
speci.al srrains-iot r"r.o"yti"
llpercrophy
is listed as an uirclassified
piocesses. Ttre eEiotogic agenr';i-;;;$i"-i"r""ti6ir
life in non-neural tissues
natal
posc
in
early
is iniriared
i"I"".ion
5iil:"1r,"
nonths of replicauiqr in
rnny
Afcer
tissuei.
ana inEesuinal
o"ri-"o..biy-i),tnphaiic
spread) aPPear: in-the CNS
;i;G (presrmaUly by heurqto-gengus
Iynphatic tissuell-;h;
corcex arxr
cerebellLul'
Ehe
include
sites
eipiciafly the brain stem. Ocher CNS
pathologic
The
tissue.
neural
in
slowly
Virar replication-pro"""ds
,pi."l-""|a.
siF9.
clinical
resultant
wich
Iesions are restEicted to Lhe neivous sysEem,
modeof cransmission.
aliuenrary cracr is dre upst plausable
'Goats
i#;;";-ui"-lir"
with infecEed sheep
raised
Vertical transmission has not b;;; do",-"nt"d.
(r).
to Ehe disease and may develop similar lesions and signs
mink'
and
mice
"r"-rur""ptiute animals used in ttre-stuay of scripie include haursters,
Experinental
disease'
the
Giy p"p.r" tr"u" U""n-puUfi"hed in chl pasc few years which review
concriouEor: DepE.of Pathology, college of veterinary l"ledicine, oklahoma
srillwaEer, oK 74074.
scacffi,
References:
ffil..l.etal.:Virologicandneurohistqlgeig^!indingsindairygoacs
affecred wicfr naiuial scrapie. Vet.-Pathol. L7: f87-199, 1980'
2. Narayan, o:;--procebdings of ACVPi{eering, Neworleans, Louisiana,
p. 99, 1980.
Arer. J. of Pattrol . 69; 209-2L2, L97)'
3 . I ' l a r s h , R . F . : Slow virus infections.
sysEeur- Advances
4 . M a r s h , R . F . : slow virus diseases of che cenfral nervous
i n V e t . R e s . , . L 5 5 - L 7 l, L 9 7 6 .
_ {-.
CaseIV - 9-208-80 (ailP 1758839).
abortions occurred in a research herd of Airican
Pygmycoats. 2 microslides (H&Eand Giersa) included.
LaboraEoryResults: AII fecuses were near cerm, autolysis was slight and no
sign@swerepresent.Placentaswerecoveredwicha-tan,mlcoid
exudace,cocyledonswere herorrhagic and necrocic villi were noted. Culcures of
fetal scmrachcontents did noc result in any baccerial grohrth.
Csrcrioucorrs DiaFnosis & Cmnpnts: Rickettsial abortion, due co Coxiella
.
b u rn e c t i .
ffincs:Aninitia1diagnosisofChlamydialabortionwasbasedonEhe
necrotizing placentitis and che presence of r*raE !{ere chought to be elesentary
bodies in che Giernsastained section. The possibility of rickectsia as an
etiologic agent was not considered and saurpleswere sent to Dr. Herb Scoermerac
che Rocky huncain Laboratory where FA-stained irpression suEars were positive for
Coxiella burnetti.
Actendees' Di
is, necrotizing, subacute, diffuse, severe,
placenta, pipy goat.
Etiologic Diagnosis: RickeLtsial placentiEis.
EEioloev: Coxiella burnetti.
curenE3:@agreeupncwichchecontributor,sdiagnosisand
corrrrEnts after disclosure of the etiologic
agenE. ldcsc participants,
however,
listed a Chlartydial species as their preconference etiologic agent. Bacterial
agents.listed
fecus, Brucella
listed for differential
differential
d
diagnosis included
ncluoeo Canphylobacter
brucet.l€
uaq)nyroDaccerfetus,
abortus., Brucella
Brucella melitensis
melicensis and Lisceria .sp.
sp. ltyr@iItrtar
ltyr
6i@iT-sms@f
frEe
Tffi-measuring approximately 0.5 by I.0
microns; all were Giemsaposicive. The two forms were considered to represent
different sEagesof the rickettsial replicaEive cycle. Mineralization was notd by
all and felt to be a normal finding in post parturienL placentas. A primary
lyrphopla$nacytic inflarrnatory infiltrate
also was noted in the placental tissue.
A photograph provided by the conEribuEor depicted the gross appearanceof the
placenta as previously described. Photmricrographs of rhotamine and fluorescene
coniuqated antibody to C. burnetti were proiected which substantiated the
conlrlbutor's diagnosisl- ffi':G condition'geierally is noc reported in snirnels.
Coxiella burnetti is ccnnnnly considered nonpathogenic for doresEic livesEock (3).
@isofzoonoticinporEanceinchaciEcausesQfeverinhrmarrs.
Contributor:
Marsh Veterinary Laboratory, Box 997, Bozerlern,MonEana 597L5.
References:
Tf,ennefeA, E. H. et al., Eds.: DiagnoscicProceduresfor Viral, Rickettsial
and ChlamydialInfections, 5th Ed., Arer.-Pub. Health Assoc., l^lash.m, L979.
Diseases,Charles C. Thons,
2. Scorz, J.: Chlamydiaand Chlamydia-Induced
Spri n g f i e l d , I L, L 9 7 L .
3. inlaldhalm,D. G. et al.: Aborcion associaEedwich Coxiel-La burnecti
infecrion in dairy goats. JAWA 173: 1580-81-,1978.
TIICITTIYP. O'NEILL
Regiscry of Veter inary P..icl'rology
De1>arcnentof Veter inary Pacnology
- +
Hiscor:ies
AFIP tlednesday Slide Conference - Nn. Zz
25 ltarch l98l
(AFIPu6r48s).
QcseI - I86-0039_q
-Dawley male rat had a 3.0 crn diameter suss on
cne rlgnc rumdible. The rnass was red and very firm. The rac was given .01
rnillirnrles of N-hydroxy FornylaninoiLuorene/fOO grans body weighc incrapericoneally
Ehree Eires a week for four iueeks.
case rI - o3-I435 (Rrrp L727492\.
n.
CaseIII - 80-2 (L757054).
marrobtsIIEar Eakenac necropsy frcm an adulc German
shepherddog. Clinical signs included lethargy, iytnphidenopathyand nyphera.
CaseIV - H76-46L(ArIp L667029\.
in body
.weighE.
. ^ , . E ' . .The
o # ianimal
T w l o phras
e d pr:esent
.pig,7monthsold,weighing2akg
in clinic,-treated with t<anamyEin
for onEday and
died on second day.
TI,VT$ff P. O'NEILL
Registry of Vecerinary Parhology
Deparbnerncof Veterinary PaEhology
Results
AFIP Wednesday
25 l,larch l98l
f: A 535-day-old Sprague-Dawleymale rat had a 3.0 cm dinnpter rnassOn
The rat was given .01
frdndible. The mass was red and very firn.
ehe i
millisoles of N-hydroxy Formylaminofluorene/ 100 grams oody weight intraperiEoneally
three tiures a week for four weeks.
Arreloblastic odontcrra.
is rnadeup of denEin trabeculae lined by
small basophilic cells interpreted as odontoblasts, the peqiphery of the segll9n
has areas iontaining a mixtuie of dentin and ens'rpl. The dentin is eosinophi-lic
often with internal-small linear tubular structures r*rereas the engt|Fl is either a
ancrphous substance or a lightly basophilic fibrilar material.
darkly qhophilic
Adjacent to the enarel in a few areas are elongate colunrrar epithelial cells
inEerpreted as aeloblasts.
Although odsrcoblEsts and melobiasts often are
to disEinguish within the neoplasm, Ehe preserrceof ennnreland dentin is
difficult
evidence of both cell types. Since the-mass is proliferative and fgm a 535-day-old
rat, it was interpreted as a neoplasmof mixed cell population (auEloblastic
odontcnra) rather than a hanartos (congenital odontma). Although Ehe rat las
injected with an arornatic amine, the n-oplasm was considered an incidental finding.
Cqrtributor's
Diamosis & Cqrrents:
Attendees' Diasnosis & CffIrcnts:
loblasEmra.
Codrents: there !{as general agreeurantwitb the contributor's diagnosis and
conrrEnts. The uorphblogic diagnosis of odontoameloblastona was adopted as
classif ied by the l,lt{0 (5), whiah is equivalent to aneloblastic odontcma. A
diagnosis of cmrposite odontomawas preferred by oral parltq,foglsts uPon
These tlunrs often are confusing wirh considerable overlap of
conlultation.
histologic features which souptires makesclassification difficult.
Contributor: Pachology Services ProjecL, University of Arkansas, NaEiqral
CentEi-FoTl6frjlilcological Research, Jefferson, AR 72079.
References:
f.=asn'm,-C. B. & Hubbard,G. B.: Areloblastic odontma in a baboon
Papio anubis). Vet. Path. L7: 100-102,1980.
Fffil-ton, J.: Tr-uprs in Dqrestic Anirrals, 2nd Edititrt, Ch. 7, 1978,
-
pp 242-246.
3. PeEer,C. P., et al.:
L495-L498,July f968.
odontmain a pony. Am.J. VeE.Res.,
AneloblasEic
4 . S p l i t t e r , G . A . e E a l . : Ameloblastic odonEuu in a rhesus mftey97722..
7LO
0 -7
- 7 L 3 .,L 9
l A t 6 i : 7L
JAVMA
of Che upper Alimentary Tract. lalHoBull. 53:
W.t
T,-olxrs
5. Head, K.
L976.
L45-t66,
Case II - 63-1435 (aFIP L727492).
.
Contributor's
is&
ltrltiple
granuloras in Ehe brain of a
na
sp.
- +
once were believed
to be glimas but notr are
Cments:
Tbese gr
thought to represent a reactive response in the brain to infectio-n by tox9p199ma.
'Itrese sectiqls lrere prepared frm paraffin blocks kindly provided by Dr. Cecil
and intergrades of disseminated
Jackson in 1963 and designated "glioatosis
encephalitis'r as in reference (4). He originally thought srrch lesiofft were glioas
(3) and his term glimatosis still cdr:notes neoplasia which does rpt seeo
appropriate. Case submitted becauseof historical interest to horpr a pioneer
veterinary oncologist.
Attendeegt Diapcnosis& Conments:
mderate to
tis, granulmntous, nrltifocal,
severe, brain, chicken.
Cmrte:
A variety of preconference rcrphologic diagrnses rcere suboitted
glims,
ild rpnsuppurati\te
including: Astrocltqna, plemrphic
and mrlticentric
rnningoerrcephalitie with glial nodule formation. Following presentaEion and
discussion there was unanimus agree@rt $rich the contributor's diagnosis. A
neoplastic lesion was discredited based on the uurltifocal and copressile naEure of
the lesiqr, Ehe nixed infle'rmacory and reactive cell population within the rndules,
in areas adjacent and
and the presence of ueningeal and perivascular infiltrates
resnved fror the nodules.- An etlologic agent lras not observed by the attendees.
Several attendees listed Toxoplasuosis or avian encephalmyelitis virus as possible
etiologies.
As the cqrtributor srd references indicate, the exact causieis mt
knownbut toxoplasunsis is suspected. Another reference suggests A.E. as a
possibility (3).
Contributor:
I.{adiEioi;TITt/06.
Departunnt of Veterinary Science, University of Wisconsin,
Refetences:
K. et aI.: Pathology of Laboratory Anirqals, Vol. I,
TffiiiEEk€,
York, L978, p. 383.
Springer-Verlag,
New
2: Erichsen, S. et al.: Toxoplasrcsis in chickens. II. So-called glimas
observed in chickens lnfected with toxoplasma. Acta Patb. et I'licrob. Scandinavica.
33: 381-386, 1953.
3. Hedoldt, C. F. et al.: Tr-uprsof unknownetiology. Diseasesof Poultry,
7ch Ed., Iowa State LJniv.Press, 1978.
4. Jackson, C.: The ircidence and pathology of Ermrs of descicated animals
in South Africa. 0nderstepoort J., VoL. V[, No. lz 323-328' f936.
5. Jackson, C.: Ralationship of glima to encephalitis in the desEic fowl,
and an associated parasitic agent.
-The Nature L6Lz ML'MT, L948.
hiscopathologyof epizootic gliosis and
6. WighE,P.A.L. et al.:
of dsrestic fovrl. J. Cmp. Pattrol. 74: 373'380, 1964.
ascrocytcmaEa
- 2 -
CaseIII
.
+
-
- 80-2 (U57054).
shepheTf6g.
Clinical
marrovrsrrEar Eaken at necropsy frm an adult German
signs included lethargr, lyuphadenopathyand hyphsna.
ContribuEor's Diagtosis & Cosmsnts: Malignant lyryhma.
al iniolved in- a scudY of low dose
Most dogs with marrow involvnent at
lyrphora.
canine-malignant
radiotherapy of
proved
to be-poor responders to radiocherapy.
rhe rire oi- initial workup ana smi4ing
Lphosarcortra, lyryhoblastic, bone nrarrow,
Attendees' Dtagnosis & Cmot",
canifrffi
Cosents: There was unanirrcusagre€srentwith the cqrtributor's diagnosis.
of this neoplasm froi ocher neoplasms found in bqre marrcril, strh as
Differentiation
ryeloid or erythroid myeloproliferative disease is based on cellular rcrphologSr
(2). This ne6plastic invoiveuEnt of the bone marroh,was further characterized as
suige IV ly,sp$blastic leukeuria, due to ius preserne in the marrolr gqvicy a$
prititive
6tistic appearance. Ttre alnpst c@lete abserrceof myeloid, erytirroid,
and ungakaryocytic i6ffs was felt to be secondary to neoplastic invasion. Tbe
radiotSer"pl *Ly account for sdE loss, parcicularly che regakaryocytic elsnent-s as
I'bthyl g,reegpyrcrine (ll3P)
these celli- are very sensitive Eo ioniziirg radiaticr.
suained bone marrorr srrnars frmr this animal detrDnstrated pyrinophilic cytoplasnic
sranules. This findine is consiscent $rith lyrphocytic cells of B-cell origin lfiich
Ere rhe cells involved-in the majority of leirkimia-s in dogs and htmans.
Concributor: Dr. G. A. Parker, lll0l
StreanviewCourc, Great Falls, VA 22066.
References:
B.: Laboratory l'tedicine: Hesratology,5th Edition, C.V. MosbyCo. '
fffi'fe-;].
St. Lpuis, L977.
2. Schalm,0. W. et aI.: Vecerinary Heuratology,3rd Edition, Lea & Febiger,
Philadelphia, L975.
3. Siuire, R. A. et aI.: Clinical and pathologic_study-of_canine_lphma:
Clinical'staging, cell classification, md therapy. J. Natl. CarrcerInst. 5l:
565-574, L973
4. Cor^rall,D. E. et al.: Effects of low dose, total-My_irradiaEion on canine
bone marrow function and canine lycphora. Radiation Res., l98l (In Press).
5. Harvey, J. W. et al.: Weli-iifferenitated lyuphocylig-leukerniain a dog:
Lorgterm survival without therapy. Vet. Path. 18: 37-47, 1981.
- 3
Case IV - H75-46L (AFIP L667029\.
loped pig, 7 trpnths old, weighing 24 kg in body
weighL. The animar wag present in cLinii,
rreated with kanamyEin lor one day and
died on second day.
Laboracorv Resulcsl
Contributor's
porc:ffiEtiology:
Culture of the kidney - posirive
DiaFnosis & C@nEs,
for Staphylococcus sp.
CorEical necrosis, acute, severe, kidney,
Staphylococcus
sp. infeccion.
CflI|EnEs: ffifffiliings-:--In-@rous,
flat erythprras were scattered throqg[nut
tfe 9!in, mainly on the ventral porticr of che boiy, neck and head. ttre surflce of
che kidney: contained scattered diffuse pinpoinc to matchhead sized reddish spots;
the rernainder of the surface was damasg6igd'abruptly by a yellorish gr.y
U.pongpening th9 kidney, the redulla was essentiilly nirrmai. The coiri-co-redullary
"oo.l-
junctict was red wiEh ccrgestion and hemrrhage hrtri;h extended out alons the
vessels into the cortex in flnger-like projecEions. The majority of uhE cortex Has
dull and pale.
Histologic findings: l,larked fibrinous thrdi
srere present in
several large vessels at the boriler of the infarct.
These vessels ilso had
fibrinoid necrosis of their walls. The necrosis of the cortical coponents
corrsisted of gloeruli,
tubules, interstitirm and vessels. Few polyilrrphonrrlear
Iercocytes hrere present at the border of the infarct.
EEiology: Staphylococcussp.
csuEncs: @ith
the contribucor's ciiagnosis and courrEnEs.
Disseminated intravascular coagulation brasthe preferred eEiologic diagrosis. An
eEiologic.?g-ent was not apparent in the tissue sections viewed by
the lttendees,
-Exoroxin.uEdiaied
buc Lhey did concur with the etiology given by the contributor.
DIC was the probable pathogenesis involved in this lesion.
Contributor:_ Delrartuent of Veterinary Pathologr, Pig ResearchInsticute of
TaiwbiJ6[ff'Iid
of ehina, Miao1i, Taiwan, 350.
References:
f.:[Ifetr-].
C.: 'Ihe Kidney: Medical and Surgical Diseases, 2nd Ediricr,
Grune & Stratton, NewYork, L962, pp 635-640.
2. Boucoc,N. G. et al.: Bilateral renocorcical necrosis wich recovery.
N e wE r g . J . Ib d . 2 5 7 : 4 1 6 -4 1 8 ,L 957.
3. Casper, J. et al.; Bilateral corEical necrosis of kidneys in an infant with
favism. An. J. Clin. Path. 26: 42-46, 1956.
4. Hani, H. et al.: Bilateral renal corcical necrosis associatedwich
esophagogastriculcers in pigs. Vet. Parh. L7; 234-237, 1980.
5. Thal, A.: Selective renal vasospasmand ischemic arenal necrosis produced
experirentally wiEh staphylococcal toxin. Observations on pathogenesis of
bilaceral cortical necrosis. Am. J. Pathol. 3l: 233-'259.f955.
.|IMMHY
P. O'NEILL
Capcain, USAF, BSC(VC)
Regiscry oi Veterinary Pachology
Departrnent oi Verer inary Pathology
- 4 -
His cories
A.FIPWednesday
Slide Conference- No. 23
1.April l98l
Direct Blue #6 ac 1500 ppur in Ehe diet
CaseII - l[66-f2
previously.
Case III
ior
(nfrP 178078).
nnale AsErican foxhotrnd was euthanized after showing
ferale litcermace had died 2 monchs
Both dogs had enlarged livers at necropsy.
- PL
89L92 (eflP
L7L3374).
le, Siaurese presented as an eu'ergency wich
sevei@ea
and abdominal brearhing.
Ausculcacibn of the chesc showEd a'nniffled
tachycardia,. no-audible L"rng sounds over the left lung fietds and lung sounds
Limited to che dorsal posterior right lung fields.
The animal died during
radiographic procedures. Ac necropsy, there was serosanguinous fluid in Ehe
choracic and abdmrinal cavities, the lungs wgre atelecta-ic,
and the liver was
severely corrgested. The hearL was sligntly
enlarged and the endocarditnn of tne
left ventricle was thickened and whitel
(AFIP L7L3947).
Case IV - EN-78P1132
red alive beEween2'20to 235 riays oi
gestation._ The fetus was weak and experiencedrespiratory difficu.lcy and dieC
shorely af cer birth.
Its lr,rngsfailed to collapse- and were rmiformly f irmer lnan
normal. All lymph nodes examinedwere 2 to )-.5 cines normal size. The cuc surface
of lycrph nodes revealed a Ehictcenedcortex. The adrenal glands were enlarged and
the adrenal cortex was 3 Eimes iEs expected chickness.
TII'OIT{YP. O'NEILL
Captai,n, USAF, BSII(VC)
Registry of Veterinary Pachclogy
Departnenc of Veterinary Ppthology
Results
AEIP Wednesday
I April l98l
Case I - 77L075
P L709298
Direct Blue #6 at 1500 pf
in tbe diet for
6 weeks of age.
Cqrtributorrs Diagnosis & Cmrts:
0va1 cell hyperplasia, (also in sc
sectfons - focl 6f Ce@
neoplastic nodules, and/or cholargiofibrosis.
Crents:
Ttre cheurical contains benzidine, a htman carcirngen. Hepatcellular
lrere
carcinmas
seen in a few rats. Later they would have developed in many
other rats.
ldoEe: ltre slides were prepared frm several different blocks. Oval cell
hyperplasia is ttre prlrnary lesion of interest.
Attendees'D
llasia, bile ductules (oval cell hlryerplasia),
diffuse, uoderate, liver, rodent, rat. 2) Neoplastic nodules, mrltifocal, liver.
4) Cholangiofibrosis,
3) Foci of hepatocellular alteratiqr, unrlcifocal, liver.
mrltifocal,
minimnl to mild, liver.
Cments:
the disucssion of the changes observed in the liver centered ort the
I'bst investigators aBree EhaL the origin of the oval ceII
oval cell hyperplasia.
apitheliu
and thus the preferred cerm bile ductule cells.
is the bifiaiy
differ fro hepatocytes by containing small oval
these
cells
Ultrastructurally
and a
small mitochondria, rare cilia,
nuclei with incoispictrcus or small nucleoli,
of rough endoplasnric reticulm
basrent membrane and ffre absence or scant munt
Ttre physiologic role of tne bile ductule cell is
and glycogen granules (2).
similat to ttre bite drrcts that ii water and bicarbonate absorbtiqr and secretion
Proliferation
of bile
(3). Tfre horrpne secretin influernes their ftrrction.
in several pachologic conditions (3).
durctules has been descriH
Contributor:
National Carrer Institute,
National Institutes
of Healch,
BethEEdSfll6rlfand 20205.
References:
Scanning electron rnicroscopy of proliferating
E. et al.:
f.TrooEs--tanley
L975.
33:
3Il,
Invest.
Lab.
bile ductules.
fate of proliferated hepatic ductal cells
and
al.:
Onigin
2. Grishan, J. W. et
in the rat: Eleccron microscoPic and autoradiographic studies. Expt. & lblec.
PaEh.3; 242-?6L,Lgfu.
3. Schaffner,F. et al.: Eleccronmicroscopicsrudies of normaland
proliferaEedbile ductules. AsEr.J. Path. 38 (4): 393-410,April 1961.
Case II - LLL66-12(AFIP 1713078).
e Arerican foxhor.md was euchanized after shcring
' # ' '
signs of asCites and anorexia. A 9-ncnth-old fesrale lictermate had died 2 Mrths
previously. BoEhdogs had enlarged livers at necroPsy.
Cqrtribucor's
IeptoE'ffiffi-
Diagnosis & Cqsents:
Chronic-active
hepatitis
associated wich
Corents:
Significant findings at necropsy were an enlarged firm liver and
ascites. A diagnosis of chronic-active hepatitis was madeon tbe basis of l) acute
and chronic inflasmacion of periportal areas with sre extensian into lobules;
2) focal disruption of periportal limiting plates of hepatocytes by this
inflasratory infiltrate
wich spotty necrosis of individual hepatocytes; 3) mifd
periportal fibrosis and bile duccular proliferatiqr.
The lesions in this
parcicular dog are rnre acute than those in other cases reported in chis series,
with a greater proportion of neutrophils and little
fibrosis.
Aburdant spirochetes
were dmnstrated in the liver by t^larthin-Starry stain. there were
no significant kidney lesions.
Hepatitis, chronic-active, diffuse, mderaEe,
Attendees' Dragnosis & Cmnts:
live
.
Etiologic Diagnosis: Bacterial hepaticis.
Etiolosy: Leptospiria sp.
agreernnc with Ehe contributor's diagnosis and
CqrenE6: ffius
cmrents. Other changes in the livei were regenerating hepatic nodules, diffuse
Iyuphatic and rnrltifocal sinusoidal ectasia, &d thronbosis in a fan large vessels
in soe sections. As poinEed out by the conEributor, Leptospiral infection nay
cause this hepatic lesion (3). Chronic active hepaEitis also has been prodrred
experisEntally in dogs with infectious canine hepatiuis virus, obstrr.rctive jaurdice,
and occasionaily has-been associated wich drug ailministration in both hrmansand
dogs (1,2,4). Corparatively, this lesion resembleschronic hepatiEis in htmans
infected with hepatitis B virus.
Contributor: Division of CouparativeMedicine, Johgg,HopklnsUniversity
Schoolfl6FF6?fi[dne, 720 Rucland Ave., Baltimore, MD 2L205,
References:
r@T.J.eta1.:ChronichepatitisintheDog:TheroIeofitrm.r'e
factors. JAWIAf56 (l2): 1700-1705,L97O.
2. t*byer, D. J. et al.: Obstructive jaundice associaEedwith chronic acEive
in a dog. JAWA t76 (f); 4L-M, 1980.
hepatitis
'
R. eE aI.: Chronic accive hepatiEis in EhedOg.
3. SCrobeck,'."D.
(4)
: 380-386, L978.
JAm'lAl73
4. Bishbp, L. et aI.: Chronic active_hepatitis in dogs associated with
Am. J. Vet. Res. 40 (40)2 839-AA, L979.
LepEospires.hepacitis in a do8. wsnc , 42L-422,
5 . - T o th , D . M. e t a I.: D rug- induced
March f980.
-
L
-
Case III
- EPL 89L92 (AFIP L7L3374).
le, Siaese presenced as an en€rgencywich
severEdyspnea and abdurinal breathing. Auscultacion of the chest shor,ved
a m..rffled
tachycardia, no audible lr"rngsounds over che lefu lung fields and lurg sor.nrds
limited to the dorsal posterior righr lung fields. The anirnal died during
radiographic procedures. At necropsy, there was serosanguinousfluid in the
Ehoracic and aMmrinal cavities, the lungs were aLelectatic, ad che liver was
severely congested. The hearE was slightly enlarged and the endocardiuurof the
left ventricle was thickened and tnfrrite.
Concributor's Diagnosis: Endocardial fibroelastosis.
AEtendees'sD
is&
ic proliferation, endocardial, diffuse,
rnderate, left ventricle, heart, Siauese, feline.
Etiologic Diagnosis: Feline endocardial fibroelastosis.
Primary or secondary.
Etiology:
CamenEs: Feline endocardial fibroelastosis
is characterized by diffuse
fibrous and elastic thickening of the endocardiuun in the absence of uryocardicis
myocardial necrosis.
Secondary ventricular hyperErophy, usually lefc sided,
accorrpanies Ehis change. The disease may be primary or secondary. The prinary
form has a fasrilial incidence (autosunl recessive gene) and rnst often is seen 1n
Burnresecats.
The secondary form often is associated with cardiac ancmalies
(i.e. aortic stenosis).
The proposed pathogenesis of the primary form involves
subendocardial edana, dilation of lyuphatics, endocardial fibroelastic
proliferacion and degenerarion of Purkinje fibers secondary to the fibrosis.
Ttris
latter feature is the suggested cause of acute cardiac failure.
This disease in
cats has been conpared to several diseases in man wich similar histologic
feacures.
Contributor:
HernZl-oi1ff07O.
Experirnntal
Pathology Laboratories,
Inc.,
P. O. Box 474,
References:
I-TfioE-f.
S. et al.:
First reporc of the occurrence of neonatal endocardial
fibroelascosis in cats and dogs. JAVl,lAL33: 27L-274, f958.
The pathogenesis of endocardial fibroelascosis in
2. Paasch, L. H. et al.:
Buruese cats. Lab. Invest. 42 (2): L97-204, 1980.
3. 7nok, B. C.: SosE sponcaneouscardiovascular lesions in dogs ard cats.
Adv. Cardiol. 13: f48-t68, L974.
_
J
-
Case IV - EN-78P1132
7L3947
ne
Eed alive between 220 to 235 days of
fetus was lreak and experienced respirarory difficulcy
and died
h-ngs faileil
to collapse- and weie unifornli
f irrer
ckran
exired were 2 to 2.5 tirres norml size. the cut surface
of l14h nodes revealed a thickened cortex. The adrenar gtands were enrarged and
the adrenal cortex was 3 tires lts expected thickness.
,
- ^ . . . @C
o w -p o e i ticarcl
ve fo rB rtre 1 1osiswithcar dAgg1utin8ti6Teet.
- Neg_aEive.
!'eEus
tg9t, negative C.F. test. Elevatiqr of lgs-in serLm.
B.
abortus
biotype I frm abmasal fluids, l*g, and internal illac
$ecoyery-of
r)t4n rKEe.
l
r
Cqttributorre
mrtr
Diqgnosis & Crents:
Pner.mnia,
diffuse intersritial;
rosiJ
Crents:
Ttris.case. represents the tlpical pattern of pnermria in B. abortus
lnfected fetuses whictr abort beareen rtre 2Oottr r; 240rh day'of g""cati*TE'i"-reactiqt is observed in experimntal infectiqrs in h'hich t[F fefge is irgulated
Pe|- os or I.l'1. Neutrophils are no!_ a prqninent cellular cqonent of these
inflauratory reactiqrs.
occasimafry i severe arceritie rg iroted-in-puidary
arteties.
In these lnstances macrophagesare still the predoinart iirfra-"t6ry
cell.
Attendees I
is&
c ulagnosls:
ia, nonsuppurative, diffuse, mderate
Etiologic Dlagnogfs: _Bacterlal pneuurcnia.
Etiology: Brr.rcella abortus.
CmnEs:
lfiffiFas.ffi?ifference
in opinion on rhe rcrphologic diagrnsis.
Tle majority, hoeever, considered the interstiEiat involvwrt'to
bE an exEensiqr
of the-bronchoprer,wria. Ttre history and lesions are quice suggestive of fetal
Brucella infectlon.
Pulmrary infectlon is via inhalafion of frfecriotrs fetal
fluids in utero. Evidence for inhalation of fluids is subsrantiaced by the
Presence of rccasional sloughed squanousepithelial cell within airwayi, a feature
also observed in normal fetal lung tissue. Other lesions observed in-tissuee of
aborted calves due to BrucelLosis are granuloras in the spleen, liver, erd brryh
nodes (1), neningoencephalitis (2), anil adrenal cortical hyperplasia dtp ro ietat
stress. A necrotizing placentitis accoryanies Ehesefetal- iesions.
Contributor: loulsiana State University, School of Veterinary l{edicine,
DepaiGrrlifrffi'tholog;r,
Barqr Rouge, LA 70803.
References:
f. .fu66,-R3/.F. & Kennedy, P.C.:
Pathology of Doestic
1970,pp 528-530,1970.
2. lbdnesdaySlide ConferenceResults#20, CaseIII,
Aninrpls, Vol. I,
f98f.
TTIfiI{Y P. O'NEILL
Regiscry of Veterinary Pathology
Deparurent of Veterinary Pathology
Histor ies
AFIP Wednesday
Slide Conference_ No. 24
8 April lggl
CaseI - 79-223L (AFrp U14909).
of a 6-year-old fernale goat wt'richhad shor.a
:]**
a Progressive loss of weight, a deireased
prodrlcion-*a-*?t"rlces
over a period
of several weeks' Finalli, ine animal
becanreemaciated and very weat(and r.ras
descroyed.
Case II - Anirnal Health Lab
c lin
IS
(live) for necropsy wich a
of-ll:g"::i::., p",:Se! l_Ad";;;';6"il'#;";;IiiJ';H
-!"
_
and
ir'"iir;o"Xio"S#::
scory
sEuck in crop
L7L33L7).
y?:,pl:t:nted
.
At necroDsv,,there were pale subcutis and musculaEure,
edematousheart,
uultipre wiriliStr nodules-i" i["-ci,ril-;;gil,"ernpry-sromach,
grearry enrarged pare
liver-wich rultiple *r,iii"tr ioJi ou". che-surface
and
kidneys
ind spreen were
enlarged with whiEish foci over the surface.
case III
- {-I228!_(AFIB L755475).
hudsonius, (sea lrorse).
Case IV_- 6497-79 (AFrp L7t7Z64\.
rsratized turrDr, 4 cm in dianreter, hras reupved
r-^*Hi:
rrom Ene ri'ght peccoral region. The tr.rnor
involved
suucutaneous
cissue, was werl encapsulaied and vascutrri;;;:"ut"nuJua-ano
smre
fibrous rissue vras nored.
TIIOI]HYP. O'NEILL
Registry of Veterinary pachologv
Departmencof Veterinary p"rhoGgy
ResulEs
Conference - No. 24
Slide
Wednesday
AFIP
I April t98l
caee I - 79-223L(AFIP U-149Q9-L
eji-ni'
of a 6-Year-old -female goat rrrhichhad sturn
a Period
a proqresstve ross of weiglrt, a iieireased prodrrcCion and sofc fEces over
was
and
wreak
very
and
Finall!, itre anirqpl becm enaciated
of'""i.i"1-weeks.
destroyed.
#
Cantributor's D
is&
Johne's Disease.
enteritis.
-Uy[oU"ctif
is, Joture's disease.
tufi paratuberculos
ili;l"S r
thickeniry.of the rucosaof the
[t necropsy,e'n"ciffid
crents:
Ioner snall lngestlne, cecu! ard colon wererpted. the u6in nicroscopic.intestinal
lesion was in ctre-upilr-ana mtOaleparts of the lntestinal tract. Granulcnatous
ihe upper and'qrddte parts of the mall
enteritis wasnlid-55i-*tiilo"ai-i[
;d aiifuse md severe in the lixer small lntestlne' cecu! ad colst'
i;;;ili;,
io rhe lmina propria of the affected intestinal
Ttre leslqrr *"" riiit"A *i"ii
involvemlnt'of the suhrcosar E"Iopfaggg in
orftn onfy-;iid, *ittto"f
;;r;6
Jotne's disease
*ssiu" nrs"rg of acid fast bacilll.
intestlnal lesioni
"*,dro"a
losses in strre goat herds. Diagnostigi3ns ehould alwayskr:ep
ffi;tlg;-ioporcing
in mid rhat Jotrn";r rfghi Ue pr"s"tt in adult -goapsdrigh have been sufferlng a
- ,r ,rrr; Jeoiljrr",riqg diEeasewith or without diErrhea. Gross lesims in the
oft"n mt very gvfdent and can be nissed
i,ir,.i.inai ti""6-;fi ;#;-ioais
"ri
wlth or'without caseousrecrosls ard
easily. leeenteii"-fyoet q6ag
"nGig*nt
anlnals. For theee reasons, p sfntrld
ii"aiG-i"'ia;.
rr-i-"ffin
calcifica.t*
intestinal tracts and reeenteric 1l4h nodee
"G;i;;-i".-ttiltip"rtr6f"grc"fTy-it.
,ltt n ot6er iefuone such ag cas@uslphadenitie or intestinal
chesegoaEs
of
parasitism are "ver,
Present.
Atcendees' Dlagpeis
i
& C@nEs:
, granulmatous,
diffuse,
mderate
to sevete'
jeJr,rnwt,caprtne, goat.
and
cmgntsi There was unanimusagreelrentwith the concributor's diagnosis
s9tre
conuributor,
the
by
rn addilion io the feacurEspointed ou9
c,F".il;and Lonsiderablenrmbersof eosinophits and
secriong
"usc"""."
"*t"in"d-"iypiLiur,
Lesrdrs of Jotrre'g disease in.goats nay-occur in mre
,irrrii"i-r;il;a-g6i
tnreJrine in addition to the dtstal small inEesEine,
proxlusr areaa .ii it.-"*il
in caErle. Leeiqrs my cqrtaln eirher few or nulErous
E"""illia-;;fi-;"-obeerved
and
acid-fast bacitrl. An additiurar-iesio.toccasionally obgerved.in sheep,catEle
of !F *ocardiimof :l:-Y:: *{::,3:t''
io"ir-'uirt'-jot'n";r_iq-Fi;iiicotron
of qftich has not been elucidated. Mirpr antigenic variatiqts
?#-pJhJj";;iJ
lnratuberculosis
exisr befweenUovine and caprine },tycobactgrir-m
(4) '
Cross
berM
l'rnvig
ffi
paratuber"ubffirE@,
1"1.raaa*,1-ora,,lno{a
reaction betweenM
Eor che
tesr
reliable
E IIDSE TEI1A
iiuE *ia-tis-is-th-r;cst
reported. lecal ElT
have
delection of Johne'e diseaee Tt
Contributor: DePt. PaChologleeE l'{lcrobiologie, Faculce de redicine
veceiTnar-idllfrfiere i re de lbnEreal, C""" po"tafe-5000, Salnr i{yacinche, Quebec,
Canada JZS 7C6.
Referenceg:
TG-ssl-F
J.: Paratuberculosis (Johure'sdisease) in goats. Proc. Sheepand
Goat Practice Syuposirtrr. Colorado State Universiry, pp 26-40, L976
2. Fodstad F. H. et al.: Posturtem examination in the diagnosis of Jotne's
dtsease in goats. Acta vet. sced. 20: L57-L67, L979.
3. Gr-rrnarsson,
E. et al.: Cultural and blochemical characEeristics of
Eycobacteriwr paratuberculosis isolated frqr goaEs in l{orway. Acta veE. scand, 2Az
uz-Ly, L979.
4. Grrurarsson, E. et al.:
Analysis of antigens in mycobacterirm paratuberculosis. Acta vet. scand. 20: 2W-2L5, 1979.
5, Jubb, K.V.F. et aI.: Pathologyof Dmstic Animals. Vol. 2, L970,
pp 135-140.
Caee II -
cLi
sCnck
L7L33L7
pFesented (live) for necropsy with a
Ffstory of eickness for a period of 3-4 days, r^rouldnot swallow food crop and labored breathing. The bird was euthanized and posced.
At necropey, there were pale gubcutis and ulrsculature, edenntous hearc,
uultiple wtritish nodulee In che throac region, eflFEystonch, greatly enlarged pale
liver wiEh sJltiple whttieh foci over Ehe surface and kidneys and spleen were
enlarged wlth whlttsh foct over the surface.
Laboratory ResulCs: Beta hemlytic Streptococcussp.. E. coli and Klebsiella
sp. ffie
isolEEfffilung, kiiney @eEE'tG
6'e chroat masa.
l,lyeloblastosis.
(both incra and extravascular
liticrs
chiefly aror.nd portal traccs) of neoplastic cells of the granulocytic series with
manymltoses and packed with acidophilic granules are cflpatible wich granulocytic
(myelogenoue)leukoeie. These cellg also were eeen in the kidney ard spleen. Thre
natural occurrence of ryelobl.astoele is very rare srd this riould be che first
natural case reported in a pigecr.
ContribuEor'e Dlamosis & Cments:
Attendees' Dlagnosis & Cqrents:
tosis, liver, pigeon.
CouEnts: Rn inflauatory etiology was discrediced on Ehe basis of
distribucion, horcgeniclty, urltotic activity, ard lack of hepatic necrosis.
Similar hietologic-lesions algo were projected inrrolving Ehe kidney. -AtEerdees
familiar wirh the normal hieEology of young birds stated the hepatic infiltrates
were not characteristlc of inflo"rrnEory lesions or extrtrnedullary heoatoPoiesis.
Avian leucosie virus (retrovlrus) wae the proposed etiologic agenEand ovarian
Viral isolation, serutr antibody, and eleccrqt
transmission the spde of infection.
microscopic studles would be helpful in Ehis and similar cases"
Contributor:
4901-6[i6Fffi-d,
Maryland Deparmrt of AgriculLure, Anirnel Healrh Laboratory,
College Park, I'D 20740.
- 2 -
References:
T--.ffi6m,
H. G. et al.:
p. 445.
2. Nbulcon, J. E. eE a|.:
Case III
Diseaseof Poultry, (Hafstad, et a1.) 7th ediEion,
Trsnrs in DmstiC
- X-U280 (AFIP L755475).
CqrtributoE' g rPiagfrosis & C@mnts :
Animats, 2nd ediciOn, p. 186.
hudsonius, (sea horse)'
Sphaeropsidales infeccion, probably
Phor6-ffiEdfrfr.-
sea horse wae injured by a hermit crab. ltris lesicr developed
ffiis
and Ehe sea horse died. Other fish in the aquaritm are
iniury
of
Ehe
aE the site
normal.
Attendeegt Dlaxpges & Crents:
s, -cellulitis, uyositis, .stmtatitis, ard
f
to diffuse, with nurcrous fungal
uurltifocal
panopth;hitis,
sublcure to chrqric,
hyphae. head, Hippocapus hudemius, sea horse.
mycosis, head, sea horse.
Diffia'ted
Etiologic fia@
Nof all the described rnrphologic lesions hrere Present in sre of
Cqts:
che submif,red secgiorts. The inflsnacory infllErate consisted-of heterophils, ard
macrophages. lbrptrologic features of .6g_frqgua were size (2-4 u), regular
seoggtio;. dicocripus branching, and parallel walls. These characterisuics are
eulgeeti're of SplraeropeidalesInfecti6n, Phma herbansr.
-mF@us CulEure o{.Eh..ftrngus
!r3s considered an
*oiTa be rerquirbA for'poaitive tdentif icaE[6t
traua.
Nurerous other species
prevlous
the
pathogen
as a result of
opporCrnrietfc
pathogens
std
others as
pilnary
as
sorrE
oi'f,rnrgf affecl aqudriwr flghes,
(1,2,3)
.
opporttrnistic organisms
Contribucor:
c@ld,
DeparttrEntof Pathology, Division of Toxicrllogy, EIi Lifly
IN 46140.
References:
l!'u.
L. et al.: l4ycotic infections. JAWA U7 (It): Itl0-ll!?,
TlGIffiiEz,
2. Robercs,i.. J., Fish Pathology, Bailliere Tindall, London,-Pp205-211.
3. Wolke, [. E.; PaEhologyof bacterial and fungal diseases_affecting fish
In The parholosy of Fishes, W: E. Ribelin et al (Eds.), Univ. of Wiscqtsin Press,
Ma
l -
frm Effiifiht
in dianeter, erasresnved
pectoral region. The tr.uDr involved cucaneousand iubcutaneous
Elssue,
Elssug. was
was well
well encapsulated
encaosulated and
and vascularizedvascularized.
Srrn fibrous
fihrous
ficque wrq nnr-ad
tissue
noEed.
ScrE
Cogtributor'g DlaFnosis: Yaba tr-mr virus.
Attendees' Diagnosis & Cqrents:
tive fibrohistlocytosis, dermis, skin, baboon.
Etiolo{iic Diagrmsis: Yaba tr-rcr virus.
Etiology: Pox vlrus.
Couents: A nmber of equally acceptable precornference diagnoses were
suhltted.
Thesa included dermal histiocytoeib, histiocytic pseudorurDr, ad
chronlc ulceroproliferatlve dernatitis.
ihe exicr celluiar dirivarion oi cells
involved was not resolved. Prollferating resoderml cells are primarily involved.
Fibrocytic cells are mst often described ln the literature,
Viriably Sized
egsinophilic intracytopl,asrric lnclusiqx, wrereobserved in the ce}ls. Regression of
Ehe Euupr was evidenced by the lnixed inflqunn+gqiy infiltrate
observed
rultifocally-.
There are four pox viral diseaees-affecting urkeys:
snnll pox,
benign.epldermal
pox
mrkey
and
pox.
Yaba
Gross
and-microscopii
Po|,
YDnfey
lesiqrs of chese diseaees in rurkeys were diecuss;d. In addition co skin'lesions,
lYtl8 tggrg have been produced exp,eriurentally ln urkeys wich aerosolized yaba pox
virus (5). Several species of Olil l,Iorld rcnleye are susceprible to borh
experiuental and natural infecticr with yaba pox virus (3).
Contrlbutor:
AFAIAL/TIIP,I^lrlght Patterson AFts, OH 45433.
References:
f:ffi,-D'.-E.:
Spontaneousviral diseaee of nonhuuanprinates. Pachologyof
Laboratory Animals Course, AFIP, 1980.
Experlrental_Yaba ild q"nign
- epidermal mnkey pox in
. 2. KupPer, J. L, et al.:
rheeus mrkeys. Lab. Anim. Care 20 (5): 979-988, 1970.
3, Niven, Janec S. F. et al.:
SubcutfiEous "growths" in rurkeys producedby a
poxvlrue._ J. Pathol & Bacreriol, 81 (1): 1-14, 1961.
4. lilolfe, L, G. et al.:
Iuunnologic resporurcof uurkeys to aerosols of Yaba
virus. Jt€I 4f (5): LL97-L202,1968.
5. Irlolfe, L. G. eE al.: Experirencal aerosol transmisslm of Yaba virus in
wtkeys. Jl€I 4l (5): fl75-f195, 1968.
TT},T]ITTY
P. O'NEILL
CapEain, USAF, BSC(\/C)
Registry of Veterlnary Pathology
DeparUrent of Veterinary PacholqSr
- - 4-
rlistor ies
AFIP WednesdaySlide Conference - No. ).i
15 Aoril l98l
Case I - 8776 (l f{6r8, I l^lartnin Starry) (AFIP 17713}4).
in a breeding colony of
LEWrats despite occasional treabrent hrith teEracycline over a period of years.
Dyspnea, r.rnthriftiness, deaths during anesthesia, infertiliEy
and occasional
stillbirths
had been noted. Five adulc and 4 weanling raEs showing dyspnea were
submicted for evaluation.
2 microslides included.
Case II -
28933
us urcnkeywas used as a breeder.
She arrived in the
was bred on 28 April 1978 and aborted on 13 JuIy 1978.
Tuberculin tests had been cmrpletely negative unEil 3 Occ 78 when tnere was a
rpderate swelling of the palpebral injection sice. An incraderrnl test on Ehe
aMomenhtasnegative on l8 Occ 78. On 28 Dec 78 the animal was noted co be
severely ill.
Thoracic radiographs revealed nuuerousdensities in the lungs. The
animal was submigted for necropsy.
CaseIII - 5737 (nflP 175698r).
H i s c o r y : T ni rcy o u E o f 4 0 , 2-rpnth-old curkeys, died suddenly over 2 days,
AII EffiEdfrwere raised in one building and nad access to ol-d chicken and duck
y a rd s .
Case IV - 78-R-245 (AFIP U08983).
sRcuR(Sn)
rat used for geriat:i.c scudies
tlPlYrr{Y P. O'NEILL
Captain, i"lSAF,B:I(VC)
Reglsiry of \teLerinary Patnology
Depar:tn:nc oi Vererinary PacholocJ
7r
Resulrs
AFIP llednesday:ilide Conference- No. 25
15 April l98l
C4qe i - 8776 (t HtiE, L Warthin Srarry) (AFIP f77B3r+).
in a breeding colony of
@
l,EWrats despite occasional Ereauenc vrith tetritcyclirre over a period of years.
Dyspnea, rmEhrifciness, deaEhsduring aneschesia, infer:rility
and occasional
scillbirths
had been nored. Five adult and 4 weanling rats showing dyspnea were
sulxnicced for evaluarion.
2 microslides included.
_ I.aboratory Resulcs: Gross and/or microscopic lesions of MRrYin varying degrees
of.sffithroughouttherespiracoryt-]:.lctsofallanirnis."Sde
femaLeshad sr.rppur,jEi.vesllpingicis
and perioophoricis.
Plycopiasrn pul,rcnis ;uas
isr'laceci fr''un aiI uespiracoiy Eraccs und' genicil Lesions.alii,f$r6lQ[i-s
were
positive l'or Sendai, rat coronavirus and Kllham rat virus.
Pseudancnasaerurginosa
and Pasteu1"llg. Pru:tlnorr.gpl.cawere isoleeed r.rrrmrrachea of fEw
rats.
ConLrii:utor's Dia
l,furine resp trdlory nycoplasuDsis,
:Leritm, lung,.
Cmrenus: SubsequentLo che study by van Zwiecen, ec aI. (I), Ene concribucor
has studied two addicional rac colonies wich similar clinicopatholog,ic finding,s.
lfhe agents ctrrrrnn Er: all Ehree were: 1,1.puJ-unnis, Sendai virus and the
r-mcl.assified fitanencous bacteriwr.
(s) of eacn oi chese agents
T6e Fiffi-rol"e
and envjroruental. [.]ctr)rrj in pathogenesis of cne respiratory disease have yer to be
jef ined. l'4. pullgnlb rnay nitve [:een r:esponsibl.e i=or t-i:e ,:)bserved inferrilicy
anci
sti.lLbiruns, EiiFEFvirus
a l - s or m y i r , i v ec o n r r i t : r i t e d ,
'flre
sliCe suomitr-ed is a seccion of lung with lesions of severe, advanced!R.rt,
i.ncluding,: bronchiecEasis, bronchiolectasis, Iung abscesses, and empnyserna. The
Warrhin Star:ry stained section dmns[races the silver positive f ilanrentous
bacterirm in iarge numbers on relatively normal respiratory epithelir.ur.
Artendees' Di
Fis and broncniolit is exudative with
bronchiecrasis, uronchiolecEasis, chronic, multifocal, severe, associaceC;vich
f ilanencous bacEeria, l*g,
LEIÎraE, rodenE.
tiagnos is: t"furine
ffurine respiracory
respiracory nycoplasnpsis , (lRI-{).
pulsnnis and unidentified filaspntous bacceria.
l,tycoplasma
litiology:
*-.+LContnents: ffi
agreement with the concributor's diagosis and
( ) i t l t e D a c r e r i a i . s i r n k n o w n . A c c c n d e e sp r o o o s e Ca C
iirrr iiilnrii,,iuirrrr rrf fhrr i)resL.nce
.r.l-ilt-:sLlruci"
It)il:,1- ;l riur:iranre
iJrl ,-lf ffr.: ,-'ilia und Lnu:; a deiecciv: clclrirre, oi cue
; r r i u J a t si : y r f r r : b a c f e r i a , i f n o f a d i r t c c p a t h o g e n i : r o l e i n c n e l e s i o n s p r o d u c e d .
fhe gross ;rnd microscopic lesions ol :rcute and ctrronic puiirnnary IEI'I were
liscussed.
Synergism becween aumcnia levels ancl mycoplasnnsis in racs has been
established. Inreraccions wich other .rgents such as various bacceria and viruses
f'las not been clear:ly elucidaced. In;rddition to che lesions produced in the
pulmonary:rndreprotltrctive [raccs, rhinitiri, otiris;ner1ia, L.lr,yngicis,and
cracheiris are itl$o reo()rted in r:ats infected wii n i'{. pulrnnis.
2r
ConEributor: Deparcinenc
of ConparaEivePathology, University of Alabarm,
Schcdf-ffitrElTdine aird Dencistry, Uiriversicy Stacioi-,-eirminghanAL 35294.
References:
f- vanfficen,
M. S, ec al.: Respiracory disease in rats associacedwicn a
f ilanenEo.rsbacterir.m: A preliminary report. Lab. Anin. Sci. 302 2L5-22L, 1980.
2. Lindsey, J. R. et al.: Diseasesdue Eo mycoplasmas
and rickettsias.
Clrapt. 15, lN Pathology oi Laboratory Ani.unls, Beniischke, K., Garner, F. I'{. ard
. J r. . m eTs., C . ( E d s), S p ri n g e r-V e rl a g,N.Y., L979, pp f48l- f550.
i. Cassell, G. H. et aI.: Iutycoplasmal
and rickettsial diseases. Chapt. 10,
IN lhe LaboraEoryRat, Vol. I, Baker, Lindsey & Weisbroth, (gds.), AcadesricPress,
ff y. , L979, pp 243-'269.
d. .Iacoby, R. 0. et al.: Viral diseases. Chapt. ll, IN The LaboraEoryRaE,
\/6]. I, Baker, Lindsey & Inleisbrom,(Eds), AcadenicPress,T. Y., L979, pp 271-306.
(le,srr I t - LAIR /8933 (AITIP ].758836).
-*it.I'E".Ji.-ffiffieywasusedasabreeder.Shearrivedinche
c,,lorty in April L976. She was bred on 28 epril 1978 and aborced on 13 JuIy 1978.
'l'ttrerculin tescs
had heen cosrplecel"ynegacive urtil 3 Oct 78 when there was a
ra'tler,tLeswelling of che palpebr:nl injecti.on siEe. An incradermal tesc on r-he
.lSdorncnutas neg,aEive on 18 Occ /8. Lln 28 t)ec 78 rhe animal was noEed co be
,;everely ill.
fhorecic radiographs revealed nurerous densiries in the lungs.
l'llr: ,rirml. was sr:bmilted for: nccropsy.
(loncrlDuL{fr Diagnosrs & (lounr:rrEs
:
ffi-IGEiTrands.rLpiirgitis,grilnLlI(xnatous'chronlc,
inttl-Cif,rcirIto tf iffurse, se\iere, ril..trr"us
ancl oviducts, rtresus nrrnkeydue tcl
'nyc()ba(:fer
itur tubercul os is.
(;rxmenfs: Gro$s and rnlcruscopic Lesions of cuberculosls were found in
pcescapular, bronchial, sttblt-utbar and nesenEeric lyrph nodes, lung, liver, spleen,
l'nrn kidneys, perlcardrrarr, myocardiwr, uterus, oviducts and colonl The CNSwas noc
,::xi.rtiirtd. M. tubeqggloslg (hrmnn scrain) rvas isolared frorn lung, liver and
snlcen. ThTS'c.F*Ts irneresring in several. respecrs. The rublrculin tesc eras
sttspir:ious bttr ilot considered trxrsicive, two rnonths before the aninuqlts deaEn. The
riv'it-rlriricspre;rrl clf the disease was very rapid since no clinical
signs were noted
unr.il. rjh()rLry before deaclr. fhe nnnkey had been in an indoor breeding colony for
r.1,ro
ye,trs pricr co ics termirral illness.
During this rine, Ehe colony was
","1,)cn'(1". No new animal.s were introriuced and none had positive cuberculin tesEs.
Irr')m rne r i'ne of rhis animal's death (Dec t978) uncil the presenr (Aug 1980) no
' , r r l rr ' , t t ' i i t l s l r i i n f n i s
colony h.rvehitd posiri.ve tuberculin resE$ or clinical signs
,'i
r ll.n.:r;S.
'l'he
hi.sroty oli t-tre colilny and affected aninal suggesEs chaf ct"leaniinal had che
''lt'.se;rsein;rn "inactive" form frcxr che Eiue of its arrival.
Perhaps imunolog,ic
supprcssion reLated ro the pregnancy allcxved exacerbacion of the clisease.
- 2 -
oviduirind?yTy,H:?gg^PYt**i'lf:::
"r"'"s,
rronkey,nonhurnan..;;i'"t;:--ii'e"jiltiiii',-""rqTl:1::i-F5I@o'uterus.
'--'-etioi"gic
*ur#l[fi:":;;;i;:;il;".",
Utirine and ovarian urycobacceriosis'
niafiosi"t
glycobaq-!94!mtuberculosis'
Etiolo[y,
iagnosis.of supp'rative endoretriris was
Conr:nrs,
b-f ulceraEion of the
lisred b' sane parcicipanCs basei'""-tT"cofogic evidence
only presenc in s@
was
mucosaand acccnrp;tft neucrophilic resporrs6' This
phenrenqr
sections and rhe etiol5g,y was noE resolvid. Foci oi Splendore-Hoeooli
Lesiqrs'
Uuberciriosib
of
was also observed in smre secEions, whicb is noi-typi.fii
Differentially'
organisms'
proposed d concurrent iniection $rirh octr6i
AEEendees
Nocardiosis, Yersiniosis
I,t. tubercqlggle, suct.,-ai;;;;-;r-';"[ir,*y"osiE,
wich
arons
-ffi;;-grinut,rnaious
.endunide.,,TFi"iffigulc"-*"i.sy8gested'nouJever,suchorganisurs.cou}!,noc
t-llions descrioed by Ehe conEribucor arrl
h: deuonssraEed.
in
*"t" i"t"l-io Ue quite characEerisrlc of cuberculosisacicobservedby the aCcendeeE
pi""i.r"J-uy-*re donrributor denrnsEracedoccasional
:Tonkeys. A spec;;i-;il;
fast organisns within lhe granulCInas'
Presidio of
Conrribucor: l.eErermanArmy InsCirute of Research, sGRD-LLV-P,
,94L29.
San Ffffisct6fiA
Referetre.e:
Laborarory
of
;]-F. ln. 6,,Hunc, R. D.: Bacrerial cl'iseases. IN Pachology
e,a1T[E'
r
/+
631166
'
&
1419Iffr
K. (ed), r ,r 7a,- ppt370- I37t'
Aninuls, g,;Pisschke,
died suddenlv 'JVer l, davs
Z-nnnrh-crld r-ur:t<evs,
r",uifai"g and had atcess fo old cnicicen and ducx
'/ards .
(;ontr ibucor 's Diagnusis:
Hisrononas fiEl-eagrld rs.
HelratiLis, necrotizing,'iiffuse'
severe' eErology
Acrendees' Diagnosis & C-onrents:
f f i } l ' , n e c r o t i z i n g , S u h a c t l r - e , d i - . s e m i n a t e d , s e v e r e '
i-1Ytlf, lrl;l4PY,
lrvi,rn'
,;;ph;i;d;;J
lF*
rii ioloirir: l)i'lgnosis: HepacicHisuourrniasis'
tiri.',1ollyt Hisrcxncnasineleagridis'
(]crrur-nr.l;:rffiwerefeLr|.rbeanexcellencexaiilPieofcne
by actendees pro'PEed a
diseirs,:. I,furtinucleared gianc cells observed 'ccasionaLry
by-;oITL' inaracceriscic PAS
rrnrphot'gic rJiagn'sis r:f necrogran'Lonacous hepacicis
The
avian rlist6pniasis'
grsit-ive organisms were proi*"t"a ii.tn simiLar'iesions of
oi the cecal i'rorm
rrt. oitanistn 1n!.the imtrrrcance
of Hisccnnnadstvasdisctrssed' The
"y.r[-of
'Iuiluyo
in the ct"n**iEiion
tieceraki.s
saIllnarLm
:'i--,,-r-r;ffi,rii"tion
anclchickens, or exposureor turkevs E.r
of
also
by chickens iir rrre rr"n"tnirn'i.rn,ri i:trerlisease"ras
il!:ltli#llitlltoccrpie.J
rliscussed (2) .
-
] -
o
Contributor:-^.llniversicy
of Illinois,
tatroiE-foTGar-Diagnosric ledicin",
DeparcrrEntof VeEerinary pathobiology &
p. o. t*-u,
urbana, IL
6rg0l.
References:
Tffii6
A. s. et al.: Atypical histormniasis in Bobphireqgail.
.
Avi a n D i s . 2 4 (2 ): 5 t0 -5 t6 , L 9 7 9 .
2' Kemp,R. L.^1rndS-pringer,W. T.: Hiscwpniasis. IN Diseases poulgry,
of
Ed. Hofscad,
Iorpasraceunivb'rsicy
eiess, o*r;-tiza,-pp B2:di0.
,..ag€,
IV - 78-R-245_(AFrpIZ0B9B3).
--
CDR(SD) rar used for geriarric
Testicular incerstititat
studies.
(Leydig) cerl cr-s'r.
Atcendees' Diagnoses.t (ldnnencs:
ifrp'mmffiffiEicical
cell .Eurnr, EesElcIe, Sprague-Dawley
r ' , f , i - - o d e n r . 2 ) A c r o p l i y , t u b u l a r , d i f f u s e , moderate,
seminiferous cubules,
i - { i . [ ' t . . :i . ; : .
! clnlenl.li:
I'rtr:t'e was unanifinus agreerenE with
Ehe conEributor,s
diagnosis.
i'he .eoplasric r.:r?rru,vere re;rdi r.y irt.:ntif,i.r,i ,*
irr.ur*.i;;;i-a;eydig)
cerrs oy
'!r-r'r"rldees'MJltiFocal hermrrhages
anrJ tvmntrocvric infiltr"r**-*"re
also
observed
'fhe
;r' r'iJilesect-i()rts'
slrnin rnr:idence,irt'sponEaneoustwrrrr$ ,in,i experir=;;;l-'
r ) r ' t ) , ' r i J c r ro
r )fr i r h e s c L u r n r " $w R sd i s c u s s e c l . r h e i n c i ; l e n r : e, i f n p i n t * n e o u s
I .it. i,.,s
r,:ty reacn r-'0-1)d[in aged rmle Fisi:her ll44 rats,
whereas
rne
,i-,"iciencrr
uuy
be-as'lcn^l
^'
;J'i {i''+i' t osborlre-Menale.l
r:nts. t-ix;rccinerrcally,vascuJ-arocclusion, caclmi'msalcs
li'ilI r:rqtroliensltave 'llso bt:en rihr,rvnto prodrrca
i.C..f .,sl in rat.s anclmice.
r ' o n r : r i b ' t t : " r I ' l - : ct ' t ' : n n s y l v r n r aS t a t c r l r i i v e r s i t y , ' i h e
i{il.ron S. Hersne;i,r.6Jical
..,i1..i"ll[fffil
i,t. I 7ria,,l.
l:efefel ]ces:
\k-
|. (;oo(lrnn, lJ.
rrlCb. 'foxrco[. &
?_
. {lrrortnait, D.
lesions in aging F34+
rn aglng
r lsh'rr:ire-.i*:nde
I l:acs.
)
i ' , 1 rs. .
l l , t . : 1 . . ' ' 1, a
- i t , ; a f 1 . . r lanr-er
i. l'hompstrn, : i . W .
r . I ' l ; r ll.. t l . , r n c c r - I n s c .
.
;
1
'
r -'
l.J!r',, ; )t l
li5x"rrll-.ttleilt li; l. r{IloFS
i 9 6t .
-a
'
rl
r:i:,
in clre Jyra{ue-Daurley
r.rt
IL{}['HYP. 0'NrilL,L
tJ..rprt:iin,USAF,Bil(\trl)
li.tl{};;f1-y rrf Veter- lnitIy P.rtn,ti,:gv
Dcli:trtnr:nl of Vet,erinarv
ll.rt ho'i t1,5.
0
Histories
22 April I98l
ing paralysis in a 3-year-old female Dobennan.
Case II - 79-10197 (eflP l7LL324).
CaseIII - W-24L
old iryorted Fennec fox'
P r77700r
fascicularis was fornd wiEh aggressive c€elmte.
daYs
Lu., qays
DUL' tro
grtlcose but,
Eo IV
lv glucose
He resPonoeo to
hypothelTic.
erd irffi-erffi
cqratosed sr<i
@d
was-totlrcl cqracosed
6e was
He
l-ater he refused food, wasnfin*rermic and seveiely depresseii. He was euthanized.
Gross lesiars were severe cachexia, severe corgestion ind edemaof che ltrrgs, &d
mild black discoloratim of the liver.
tlase IV - 80-708-7/8 (AFIP U57060).
(ry??l!i!!ry?
E::s_
gL?
'@eseaninrals
o---:
=llEters
2-3 weeks afcer producing
-N9
died or were round rcribund
Gl*g.d
i"-"
inbred breedirg colony.
"r"ff
a^lnr'r nor
nrrr were
r.rere
animals exposed
exoosed to
to""xoerii,etttal
experirental
animals
colon!
red)
I Corgo
H*?."td
knownpathoqgnYi:_qT:",:1. il:n"
- I t[E
(2 microslides
H&E,
antigens.
antigens.
TIT'OII{Y P. O'NEILL
Resistry of VeterinarY PachologY
oefurturlnc of Veterinary Pathologr
Results
AFIP Wednesday
Slide Conference - No. 2o
22 April l98l
CaseI - 79-6573 (AFIP 1758840).
ng paralysis in a 3-year-old ferrale Doberman.
lqboqqtory Results: Postrrcrtem- extensive diffuse nonsuppurative
..---.....*
rreni@withnulrrerouscoxop1asma-likecystsincheorainand
spinal cord.
gontributor's ?iagnosis: Toxoplasnrcsis.
Attendees' Diagnosis & Cmrents:
cephalorryelitis, g,ranul<matous,
subacute co
chronic, multifocal, rrxleraEe to severe, brain and spinal cord, Doberman,canlile.
EEiologic Diagnosis: Protozoal meningoencephaLouryelicis.
EElology: Toxoplasmasp.
Ccsrents: ffiere was g,eneral agreeuent with the contribufor's diagnosis.
However, the majority of participants favored a granulomatousform of nonsuppuraErve
uenlngoencephalmryelitis or mningoencephalitis wich associated areas of neuropil
destruction and collapse (malacia). Not all the slides contained sections of
spinal cord. Profozoal cystg consistent with toxoplasunsis were present in msc,
if not all sections. Sme actendees reported intense aggregates of eosinopnils in
several foci. The inflauuratory response was considered cypical for toxoplassDsis,
although granulon forrnacion also is reporced (l).
MJltiple sysueminvolverenc
especially pulmonary is corrrcnly encountered wirh Eoxoplasuosis (2). Ttre life
cycle was reviewed briefly.
The rnst comon rouEes of infection are ingestion and
transplacenEal,
Contributor: B. C. Departunnt of Agriculture, B.C. Box 100, Abbotsford,
British Coltnbia, Canada.
References:
f.--miTlf,
D. R. Jr. & delahunta, A.: Toxoplasunsis of the canine nervous
system: Clinicopathologic findings in four cases. JAWIA159: fB4-1141, 1971.
2. Jubb, K.V.F. & Kennedy,P. C.: Pathologr of DouesticAnimals, Vol. Z,
AcadeuricPress, NewYork, 1970, pp 673-
Case II - 79-10197 (AFIP L7LL3Z4).
ofO irported Fennec fox.
Cmtributor's
Di
is&
bacilli
present within roacrophagesin Ehe liver.
Attendeesr D
Gra.,ulo-"tous hepatitis due to
ccryatible
wirh l'{ycobacterirn p.
tlere
is&
anu
, multiple, disserninated, severe, liver,
Ferurec
ennecus zerda), eanidae.
EtiolqtiE
T5T--Hepatic mycobacrerios is .
Etiology: ltycobacteritm' sp.
granuloqrascontaining nrr,.rous acid-faer
9S:!:,,
_@c!rg
bacilli
were dmnstrated.
Caseati6n"necrosis-wasnot a frminent feacure in this
case. Tbe laree ntder of organi$rs present suggested inieciiqr wittr M.
bovis or
m. avlutr. tbss
--?]r.esusceptible to infection wit6 tt. lovis and I'{. tubetcul66'G'and
E-relir6' suscEpt
ible bur iroieies i srenr ro i"iJliifr ,rIE6-M:'
iut=ffiffib.'
anddogsare reporredto re.gdfry;;il;6;i;i,
g:lT:::::g:i.l_ (ll:, _Hors,es
with sarcometouslesiqrs whereas hufons and
bovines extribiE rhe urcre classical
tubercle formation with caseation necrosis and calcification (3). The pathogerlesis
of urycobacteriosis ln the canidae was discussed. This case id very similar tc
one
recently reporLed involving several Fennec foxes (2).
Ccttributor:
Livestock DiseaseDiagnostic Center, RouEe#6, Newtqrnpike,
Lexirgcor, Ky 40505.
References:
r. Friend, s.c.E. et al.: rnfecrion of a dog
avian
serotype II. Ver. parh. 16: 38f-384, Lglg.
"irh l&gbggr"ri,m
^^- ?r-Hirygg,E. M. er ar.: Tubercuiosis in Fennecfoxes. JAWIar77:
825-826, 1980.
p.c.: pachologyof Dcresric Anirnnls,vol.
3. Jubb, K.v-.-F. r:*:{y,
r,
_l
AcadenicPress, Newyork,
1970, pp 24f
).-
case IIr - 80-2@.
fascicularis was fornd with aggressive cagemece.
He wEFFo6?I cmatosed and h@Th-e@ed
to IV glucGe bur, rrlo"days
later he refused food, was hypothermic and seveiely depresseii. He was eutnanizld.
Gross lesions were severe cachexia, severe congesLion and edemaof che tuqgs, ed
urlld black discoloration of rhe liver
Cmtributorre
Diagnosis & ComEnts: l,talaria, chronic, mderate, P. krpnlegi.
y did nol cause his deach Uut-lbFff6fr
contrlbuted indirectly.
P, knollgsi usually is clinically silent and 6verc disease
can result fron stress orTffiE-EFppnession.
On questioi'ring the handlers it was
revealed that Ehis mcrkey was dminaced by his cage mate and-denied f@d. This
resulted in cachexia and hlpoglyceuric crisis.
The Lerminal disease resulted from a
severe, PeracuEe,_
sePEic, thrdo-embolic pner-wria and uryocarditis. Ttre only
explanation fornd for this is a nosocmial infection associated with parenteral
therapy.. There also was iffrqmosuppressiol; Ilqhoid follicles ln both the spleen
and lyryh lodes were atrophic, pr6bably a resirlt of rhe food deprivation
PerPetrated by his aggressive cage mate. Other lesions notd were chronic
glmrulonephritis
of:an iruune cmplex t)rpe, R.E. hyperplasia of tyqh nodes ard
liver, pigrent_depositiur in_Kupffer cells and spleen, and focal acute hydropic
degeneracionof thb liver.
Speciation of the paiasite was perforned at 3.W.pg11n.tation
as 'htost likely P. knowlesi".
AtEendees' Diagnosis & Csrents:
i a , erychrocytic,
mild co urcderate, Macaca
=ligiculg4g", nonhrmn primate.
--'Tffifogfc
Diagnosisi Malaria.
Eciology: Plasnpdiur knowlesi.
('iouents: ffie
srnars only Ehe trophozoite form of the
parasite could be identified.
Fewer srrEarsconLained borh rrophozoites and
schizonEs. AIL of the srears contained parasitized red btood cells and fine
yelloto-.bro{rrn
granular intraerythrocybic pigrent. Specific idencification of che
species of Plasrpdim could not be madeas Ehere are a nr.ruiber
of Plasmdia which
infecc nsian uon@
Differentiatly HepaEocysEissp. mrst be inElfftlEfEcause
i a r a s i t i z e d re d b 1 i ,o d ce 1 Isa l soconta@ndg.zr e6qcyticfor msand
piguent, however, erythtocytic schiz%any does not occur. The major gross and
hietopatological lesions of malaria in monkeyswere discussed (4). Srrrp eg 66"
irmmological pararecers of the disease also were revieroed (2).
Cqtrributor:
University of Texas, IutadicalSchool at Houston, 643LFannin,
Houe6;;rfif--71m30.
References:
The Primate Malarias, U. S. DHE'[{Publicatisr
BeEhesda,
l"lD 20014.
#1744-0005,
2. Iumr"nologyof Malaria.
57 (Suppl f): 277-290, L979.
Bull. 1^ll{0
3. Loeb, W. F. eE al.:
Heuntologic disorders. In Pathol-osy oi lrboratory
, N:ET -, 16 , P. lu.Ef .
A
n i q r 4 - l s ,B e n i r s c h k € , K . e t a l . , E d . , S p r i n g e r - V e r l a g.N:-rffi
--Tloller,
In Patholq& r of Simian Prinrates
A.: Plasrndiuln and hepatocystis.
P a r t I I , K a r g e r , B a s e l , L 9 7 ' 2 ,p p 5 7 - 7 3 .
- 3 -
Case
nlsEorv:
P L757060
Three f@tgt"
(lbsocr.rcgggg auratus) g-9 npnths
aieffi
old
roundsn;b*a-I--I'*"eks afrer
'No
anirqals
belongedto a small inored bi*airrg colony. @hese
knownpathogen
-?I-ri"roirides
lras presenEin the
animarsexposJ Eo experinenr;i anrigds.
- l H&8,
fllSg"towere
Cantributor'g D
ver, al[y
severe.
is&
Kidg"y,. amyloidosis, rcderate to
ryloidosib.
ry1o
gli'arery H6E
;nd-dfi;', Ji'iJ"Ii*"
:fl5' :::. ._gnf
"Hil:::oi;:.:fr""i1:"y:"';#
- !ffiiill
;4
Regretably,
all slides do not :H":ff
trjy5-F1...*"e!;i::iffi
conrain
:_:Tt..r.
kidnev,
river;"9:ttJl. _:p?n5anegus.generalized
ip*'.';d;"
1ildilffi,"?,":m'i:'"
idosis is cmn
;:fffrffi auryloidosis
,
in older
"o,r*n
i","u"r
lffii:'.1*l;i:":":h:?,ff:,ti:iir.
:f:ii;:_ F-ifr
animalsof this age wichout'exsris=ntal inductiqr.
rltt
i ryareunexpecced
in
is&
and
is, diffuse, rcderace to severe'
kidnev,
river
:*::-6i91_ru;;; cc,i#iillsgq
,
*d;i3i,ffiH::
.ilTl,li;ri buror,
,i"i""fl3llil"rff*
i s and
:ffi'T i rrH:':' H" lli_iffi
-;1;;;:r:;r::fi;'i:
deposi.,s diagnos
kidnev
;ffi;?
.?jjl*"_t*ff:"1?l,Tl
::i::::r;i"i.'i,ryroid
sections.
srmik' Jiir*" d"p.ri;F
I;:""Fi:ff":,;
;"
i:.ffl',1?riff":?li":f***
and
infranmarion
of r.n
lll:i;,,",:"j*.ilg.-"_::f{io
lll;i;,""*"1#.ilg::*:f::i:i-":tFi;"il;""ffi
"g.i;il;b"il;-necrosis
i?::'
ril,
i;FG?""Ti:
-i0%
.#l"J3'#ffi
ii33'
ilfi
3
f;::l3:',
:""
:ff-'::=:::
i
.Ljini.Tirl^:::.*?::,fg^ryi-:d; i."is"#;
"#""ffi:':ffi""l#l:
;ffi
?ij:
;q*T:il;'
:'":3;'i:dtr
'3fiii:{o:3'fl
i:?:
53L",H:::"
il,l*::,l:.
Fiti*:-:'
11#;r.
ffi
"fi#
;:i:'
"li:;
l:i'i'
lfi
Sm;:::l;
Iî$ir
:i::
ii""i#"f,#
iii"'i;;";il il;rft ;#;H'.r':il#{#iHi
"*,'##:
procedures inflY*:i:::"'"i
:is::ffi::",
harnsters.
concribut""l-^rrttryil
Kansa-ilFacs-Ihiversiry,
gf,PattrotoFr: couege of_Verer inary l,4edicine,
vcs Blog., Manhacran,KS 66506.
References:
FCE-affi
K.: sponraneousamyloidosis in LIx mice.
Am. J. pach. 90:
3 8 r-3 9 6 , L g 7 g .
2' Gleiser, c' A. et al.: Amyloidosisand renar
paranyeloid in a closed
hnrnsrer_colony.
Aniur. sci. _ir: wj_ioi','-Lg7L.
_L"b.
3' Glennei, gr^c-.i^ Amy6ia.{9poqir" unJ'",iyroidosis, parrs
r & 2, N.E.J.M.
vol..302(23): L283-L292,
vor. 3l?'arir:.rill]ii+:,
1980.
4. Gruys,E..et-al.t- oeposiriori-.1-",rryr;iiin
che
liver of hamcgsrs:
An
enzyue-histochemical
and eleicron microscoil-"iray.- i"t:-nnii. r, l_9, Lg7g.
5 ' Jakob, I'1.: spontaneous-amyloiaosis'6r-*r.rrl--v"1.-T"rn. g:
292-306,1971.
TII'I}IHYP. O'NEILL
Regiscry of Veterinary patholoey
Deparhn:nt of Vereriniry pachoLgy
- + -
HisEories
Confererce - No' 27
Slide
AFIP tlednesday
-29
APril 1981
case I - 79050- (AFIP1749!91.
Laboratory DaEa:
Segs
Bmds
Lyryhs
Eosinophils
lbrps
Basophils
Few Innrnocytes
qt exercise'
, decreased endurarce, cough,
Urinalysis
16,400
11,480
0
2,LL4
2 ,L 3 2
246
328
SP Gv
st
Protein
L.032
6.2
2+
No siprrificant sedfuEnc
30
BTIN
SGPT 30
7.5% retention 30 nin
BSP
Knotts test: Negative
Case LI - 13576 (AFIP I76f484).
inaEety !--Ieqr;old female rhesus rcnkev EhaEwas
aira aiea before a clinical exminition could
to*dnbe perforred."ce'-wittt-Uloody'biarrhea
CaseIII
L78iL72
19643-B
I.25 cm mass locaced on the cranial pole of rhe
of a l75-daY-old SParEanrat.
CaseIV - BG-9I (arE-1291252)'-
ncs an incidenral
(l,Lacacatascrcurarls)
finding
in a 6-year-old mnkeY
.
TIWfi{Y P. O,NEILL
CapEain, USAF, BSC(VC)
Relistry of VeterinarY PachologY
of VeterinarY PacnologY
o.frtu=t,t
AFrpwednesday
rfr??it""ference- No. '27
29 Apr il l98l
CaseI - 79050- (AFIP l7lt039).
, decreased endurance, cough, on exercise.
Segs
Bands
Lyuphs
Eosinophils
lbnos
BasophiLs
Few Irrunocyces
16,400
11,480
0
2,LL4
2 , '246
L32
328
Urinalysis Sp Gv
sr
Protein
t.Jt2
6,2
2+
No signrficant sedirenc
30
BIIN
SGPT 30
BSP
7 .5%retencion Jir m' ,'.
Knoccstesc: Negacive
Cqrcribucor' s Diagnosis & C@nencs:
ticiaL,multifocaI,chronic,eos1nopt1ll.i:
nrcderaEewich an occasional microfilarlal
fragrenc presenL rn che lesron,,.
2) Endarcericis, proliferaEive, villous, rnderate, chronic lobar branches pulnonlr';r
arcery. 2) Hyperplasia, nedial, small puhonary arEeries, mrnimal, Lung.
macure adulc Dirofilaria
were reupved from che puLuun.rr'r
Cmuenc: Thirty-four
arcery and right vencricle at necropsy. In chis study 2O7.ot 34 infecced dogs ;r.rd
crypcic or occulc infecrions.
Alchough Ehe females were gravid and pr:odu'rne
microfilariae
chey were being sequescered and descroyed in che puhnna;y
circulation.
The eosinophilia and basophiLia are consistenc wich chronrc
Proceinur ia irt
paraeicism and fumnrnocytesindicate probable ancigenic srimrlacion.
Ehe absence of significarlc urlne sediment indicares gloarerular dysitrncElon. Reiiarfunccion is adaluace, i.e., abilicy Eo concenErate urine and lack of azocemra
Ircreased BSP recension suggesced diminished hepaEic olood fLow due Eo c,rroni:
passive congescion.
& Courencs:
AcEendees' D
rq6rrvp99
moderaue,pu
t
Ll
t
eraEion, villous,
Lvlr!vLservrrt
v ---vvv,
incimal., urulcrfocri
:
ry arEery, lung, canine. 2) Pneumnia, i n t e r s c i c r l l ,
eosinophilic, subacuteCo chronic, muLCifocal,miniunl, lung. 3) Hyperpl:sia,
nedial, mulcifocal, rninimal, puhnnary arceries, lung'
E c i o l o g i c D i a g n o se s: l ) P ar asiCic ( dir ofiLar ial) endar cer icis. 2) Par as i c t.
(dirofilarial)
incersticial pner.mnia. 3) Increasedpulnnnaryresiscance.
Cmuencs: There was genbral agreemencwich che cirncribucor's dragnosesand
obseived chac che lung had been perfused. Abncrrul cli,li.;al
cCIrrpncs. AEEendees
pachologyfindings $rereleucocytosis which included absolute neucropnili3,
iosinophilia, and basophilia; increased BSPretencion; slighrl.y elevaced SGPTant t
rnild pioceinuria. Ttre-eosinophilic incerscitial pneurnonia,int.:rogrsnulcnas3r:-1
microlilaria were noE reporced by rnst parEicipancs in rheir: preconierence
Ene puLrnnaryerEer.l
diagnoses. The prourinanlcollaciral ciiculation surrounding
'fne
pacnogenesisoi cne
10"s"pa.",-o=dro Le due co increased pulrnnary resistance.
lesibns were discussed, Several actendees llpnf,ioned chac an iirmuneconplex
rnediateddisease parcicipated in che developmentof che arferial Lesion, as is rue
case in che Lesioirs obseived in membranous
llolerulonephritis assocracedvtrEh
ar e r n;ar ;r s
d i r o f i L a r i a si s. Och e rl e si o n s obser vedwicE dir ofilar iasis in r he .1o9,
tnrunbi.
of Chdbrain, hearE, kidney and other organ and saddle
ConEribucor: AnimaI tleso,-rrcesBrancrr, Div. Vecerini:ry I'terlical
ReseJrcn,
FDA,-FTEETIIE;
I'D 2OTO5
.
References:
-@l..\:|--PulrnrraryarceriallesionsincaninedirofiIariasis.
J. Vec. Res. 222 655-662, 1961.2' Mtrnnell, J. F. eE al.:
rnrimal lesions of puhnnary arcery in dogs wiih
experirencal dirofilariasis.
Am. J. vec. r{es. 4Lz'rroa-rriz,-iggo
3.-Rawlingsr c. A. ec al.:
T!"^1""p_o!se of che canrne hearr- and lungs ;o
Dirofilqria
-6-rcir,-G.T-eciumicis. JMttA- It+: L7-32, i9ZU.
parasrce specif ic h.nn,,ral air.l
al.:
Dirofiraiia-irmicis;
ceILular^resPonsesinrheeffid-JG:-;6:-P;iasic'rlrl:
80-86, 198t.
Ialtrong,Ming M.: Experinnncal occult dirofilarrasls
in dogs r,ricn refe;:en;e
.5.
co ismunologic-response and its relationship co cropical eosinophilia
in man.
s.E. AsianJ. Trop. l'bd. & public HtEh.s, 4a0-aa5,'Lgii.-------r
Case II - 13576 (AFIP L76L4U+).
HrsEorv: 'l'nls ya.s-an.approximately
- --_
-4-year-old fenale rhesus rtonkeyL lgf .dr,,
tound ProstraEe wirh bloody diarrhea and
died before a clinical examinbcioncoul:
be performed.
Concribucor's Diagnoses& Cormencs:
Syndrome.ceieffiis.BV.Brain-cerebralvenousEhrombr:::i..
Brain-- perivenular demyelinacion. Brain - gemistocycicasciocyrosi.;, Brair:
malacia.
Ccmrencs: Cerebral venous chrombosis(CVf) was identified in 4 rhesus
nnnkeys. Tt^roanimals initially exhibired neurologic signs and 3 had drarrnea r)r.
dysencery. Al! 4 cases had severe incescinal dis6ase, fncluding i cases of
ulcerarive colitis; these nuy be r:elated co an underlying coiguTop"6t:- c*.,,-t,,t
nervous tiysrem leslons i"/*ft?c.oofined co rhe cencrumsemiovaleand-consisce<l
of
multiple chronbosis of incernal cerebraL veins, perivenular derryelinaEion,arul
gerniscocytic ascrocycosis. The lesions were similar ro chose fbund in hLmans,,,r,.r,
CVTand supporE che hypochesis chac perivenular deuyelinacion rnayoccur as a
sequela Eo venousocclusion. Moreover, rhe lesions appearedto 6e idencrcal rc) i',,,
pachologic changesfornd in "leuc@ncephalosis and peiivascular myelosrs" an encri:r'
of unknowneciology previously describid in nonkeys.
Atrendeesr Di
is, venous, subacuEe,mukifocal, mild co
rrcderace, cerebral corcex, brain, rhesus urcnkey, (Macacamulacca), nonhLrnanprinuc.
2) Degeneracion, whice maccer, perivenular, subacufficffi-if,
multifocal,'
moderaEe,midbrain and cerebral-cortex, brain. 3) Flineralization, vascular,
mulcifocal, nild, meninges, midbrain and cerebral corcex, brain.
EEiologic Diagnosis: Cerebral venous chrmbosis.
Eriology:
Unknown.
Cqmtencs: Noc all of che lesions above Frere present in all of cne sections.
The venous thronbosis and mineralizacion irere absenE in a few slides buc che
perivenular white rnaccer deg,enerafion, gemistocyEosis and ascrocytosts isere pr:se.']t
in all of che secrions studied by rhe accendees. Capillary thrombosis and slug,Er,.l-was observed by so{IE. Differenrially
the atEendees' eciologic diagnoses included.
l-ead poisoning, leukoencephalosis and disseninated incravascular coagulacion. LeaJ
poisoning could nor be ruled out, buc the presence r:f thronbosis and"lack of
capillary proliferation rJoesnot fic rhe described lesions oi crris condicion.
Leukoencepnalosis, a disease in rn:nkeysof unt(no\^neciolcgv rvas plausable, -no\,Jc\,'er,
cnrombosii is noc reporred, buE as che concrioucors poinE ouc in cneir puolisned
reporr (Z), chis nuly'have been rnissed in che original descriptions of cne dise;;e
(3). The parcicipancs drew an associacion between che bloody diarrnea anC cne
A coaEulopaCi"ry(DIC) was proposed as cne cause seconJai:y r';
lesions in-che brain.
At che close of rhe discussicir
sulrr as shigellosib.
a baccerial enteriuis/colicis
che etiology sEiIL remained unresolved.
Concribucor:
of Pathology, School of ltedicine, Universicy of
Deparruenc
-MD.
MaryE-nifiElEfrre,
References:
P.: Primary cerebral venous thrfftbosis in yor.rngadulcsi fn;
T:TverffiE,
diverse manifestaEionsof arr underrecognizeddisease. Ann. Neurol. 3' df-6b ' -,)' .
2. Sheffield, W. 0. et al.: Cerebral venouschrombosisin cne rnesus rlo:'riiei,
VeE. Path. 18: 326-334, 1981.
3. Van BogaerC,L. eC al.: Neurologic disease of -apesand nrmreys' ]n .
Eho
Case III
Sar.rnders,L. 2., Ed., Acadenic Press, NewYock, Lt'i-:
1.25 cm mass locared on the cranial
of a I75-day-old Sparran rac.
pole oi i
Concribucor's Diagnosis & Cournencs:Nephroblastsra, righc kidney, SparEar,rti
ryonal carcinoma, emDryonalcelr L\.t,l)t.,
Atso
aPPearsto be Ehemost cormnnlv
and Wi6s' tr-rtrr, fhe nephroblascCIna
"J"noJ"i"oma
found primary renal Eunor in rats.
Accendees'Diagnoses& Connencs:
blascoun,-kidneY,SPart'anrac'
ml
2) Gfaneruloiephrosis,chronic, mulcifocal, mild., kidney. ..
';'
gmmencs: There was unanirooJ"rgtuemencwich che cbntribucor's diagnosis
also inclilded ttte diagnosis 9f^qlffrulorephr:)sls rr}
nephroblasEo{na.AEEendees
leiions consistenC wicn Chis entity urerePresenE in manyof che slides
r:'i:
afe fhe nost cortrlonspontaneousprimary renal.neoptagyr.of Ctre ';'i'
WepfrroUfasECInas
cutrr
This
they ar- preswed Eo arise fi:mr che metinephric-blaseesu.
[i[n"y.
€ocouo'-'rr':
conrrasred ro cne 5"i"*"coid crrpr of che rac which is Less frequencl|
i-'i:r 'l
laccer
Ehe
,pont"r,"ously and can be induced chemically (2). The features of
are co{nposirion-""tiiuly of mesenchFa}-tiisue elemencsincluding fiorobl';sct'c'
adipose and osteord Erssue (2,3) '
snrcothmuscle, *6iionic nresenchynre'cells,
Li'r''
ina gii'*.ruli #v p"':*u:lfg ?{.th" sarconaroid
Preexiscing,"nJ;ifil;t
(z)
of a nephroblasccma
;IG-ai"ifig $e inicial appeaiance
Concribucor:
2oo
of Parhology, Fmd & Drug A&riris''rr
Bureau of Foods, Divisiorr
'20'204.I^l., washington, Dc
References:
I['
K. et. al.:-^Pacholoqx ofrlaboracory Animals, Vol'
ffif.€,
Springer-Verlag, NewYork, 19-78,,pp. IL/0- tri L'
in che rat: HisEology of a s1rcnEa^'er
2. Hard, G. c. ec aL.: Neprrr,lbt"asEcrna
and a review oi
cLnDr, idenCity with resPec[ to i""rf mesenchymalneoplasrs,
1976'
p r e v i o u s l y r e c o r d e d c a s e ! . . I . U a C l - .C a n c e r t n s r . 5 7 : ' J 2 3 - 3 2 9 ,
i
CaseIV - Bc-9I (nr,tp L7g3252t
.
ncsat1incii]errEaIfindinginao-year.-;Ilij}o-rKey
Colcributor's Di
is &
pentasl:cxniasis.
,ComnenCs:
vw'ErrLs;
rne renEastomida
consETEucedn aDerranc
Broup of nelmincns r.rnicn
t). t{odein
r,{odein scudies
li:*::^H-"ll::.i::-;o-Jlnur
Taior cLasses (l).
Srrrcriec ,ugg."i-;n"c
c,,oda6r F
# #:ili 1fr3;""H1:.i":.::'=,,:#T:
ff',:i":ffi:"
iF.
iH.inH.l^J:
fli:p{"
jstacus
j,:i
:i::H$3,3,"j,,i,:il"H;.t:
H:iof ff
an ffi
independenr
:s;5:n
*"il*;?i3,;iff"T..:;
pi,yi* iill
There are E!''oorders'
The cephalobaenida are-the more
primiEive and cneir liie
cvclesinvolve
fistrl-aupi'ioi"l-."i.ti.irg9-;;.fiiliy
1rytgt",
sornein tbis order
i,osr<rl. Since
can presr-unabli
gpi";;ii5*
iir"-"y"GJ;
host species, chey are ionsid"r:_9j*_nrcsc
a singre repririan
pii*iciu"
<fl. the porocephalida,
excepcingone spetit", r"quiie-marmars
as sicondary and reptiles as definitive
h o s c s( l ) .
anunsesrrEnred
body;z) meca:neri}) il.fi
Jii
i"
i:
,ff ::yrnk,,
;i
T:,
]J
ff#T":,
i
H:ll3,.:.*i
llii::y1'
i*:*TI"t"ffi:'*::*:T
lJI""o
.f;
":^;tii:Hit:;5iirl"ii3ff;?::,'o':?ff
BIlffi l;o?13,
;ffi:il #: :trT**'',:ffg*j?"
$:: -ie;lp,i;;;;".ii"li;o?iiE;
l::ii:':;:"ruS?E"p're'Ted:"":i:nf
","
;":$:ffi"J::,i::l::-{i,:l,ly_!::tls:,
m:":i:":li,TH
I'nmml
ffffi,,ffi*+,
0,.
jffi
;.
i
i
;Jffil
H,,llru"j",
I'ln
i::::'"::
*- I*;:,;t
_
l!!ii;
ii'E;:
ilT::;=
lungs and mpncum
of pri*;;;'Ci; .
rarel
v
in
rho
l
^€
^--.i-r
"'.,^-
in che tiotpf, nodes,
At leasE Ewoof che followi:q^Tl:r"scopic
rhe secEionssubmigted. ii pr"uaosegrrenredcharacrerisrics shouldbe presenco'
body;
ti-;;;i;iJi
.+)-cuiicr"-ir,".
*."""rically
arranged
muscles;
3) variou.
mfybe spiny,
srmoEh,
or shorrsigrs or annularion
""iaofii-fi;i""e;
yfSlr
;"G;;ri;"j"lpur,rngs
Fil_rit"
ro sKln
grands; 5) incesciile wim
nuureiousvilli inuoruing supporcingor connecclve
crsjrud
or 5) rwo pairs of hooks i"G"
rreaa-rett;;:"-'
l,-^^
asciculqris,
--ETI-oI%f;
nonhr-uran primace.
- - - parasice,
periadrenal
t---eY-bv'
faE,
rd
relrqvrslrcrr
Macaca
niagnosisl- 6;;.comias is.
EEiology n'nsc probably ArmiIf i fei-armularus
.
Couents:Therewasunaiffi-grffihtheconcriburor,sdiagnos,ii.i-:..::
concributor's cotrtrEntsand referencei
-'
listed were felc co u"-u-"mprehensiv* fe.,..-.,;
of parasiEe hosr relacionsrrip.
References:
ffiFT:,BiologicaIrelationships.ofrhePencasctmida:Abrblrogr.iphy
on che penrascmrid.a.Exp.-parasicol. z+, ni-tig,
1969.
2' chicwood,M. et ai.: Idencifigatign oi-iarasiric
urerazoain rrssue
seccions. Exp. parasicol. 3zz 407-5tg, Lgiz.5"" pp 4r5-4r9.
3 . I ' l o b l e ,E. R . e t a l .: p a ra si cology:Th. i
, Lea &
Feoiger, Philadelphia, L976,
OOZO'f;iff.:
Ti},fiHY P. O'IWILL
Capcain, USAF,BSJ(VC)
Regiscr yoi Veter inar y pachoLosv
Departnentof Vererinary Rachofogy
Hiscor ies
Conference - No' 28
Slide
Wednesday
AFIP
13 MaY f98f
CaseI - 80-212(AFIJ-Y57728)'-r-old fesnle Cubal.hutia,(CaprgBY-sRll*d"t)
i.ras acqul."o ,r*, i- r* in f loiida
anoreccic and showed weight loss'
f ive r'nghs earlier
Enac
and recengly Oecerp
CaseII - L5?78(ArIP-126a738)'-
collaris) was being
duck,($rchya
rirgneck
uErs,
yscrL>.T./ male,
r\;l--ftnffi
.',^'.te':':-;_----recent
in rec
0 g in
annfhor zl.\n
dropoed tr6-DEEo=mO
weight.at,oPp9d-l:31l
zoo'. weiohr
Eoanocher
-il"Ii"E
.""dffi"nifiment
signs
"Y^g
clinical
ocher
'
l'lo
bfood work revealed elevfced wtrite count
HrSCorli
ffi;il:
Ageq
\rr
qnt-ifrrnoel
, , ^ ^ ^placed
laaaA
n
n antifurgal
on
Bird-was
""i"
the inicial Presentation'
afcer ""t.a.
---r
D:-r
Laboracory Resulcs:
ffi
segLYmPh.
Hesptocrit
Tocal Protein
was
as fOund
a
eents
a
nd w
and
agents
IniEiaI
35,900/mP
92%
8%
35%
4.8 sddl
dead
d
ead
Ehree weeks
I week lafer
30,500/m P
93%
7%
30%
4.4 N dL
- 80-002 (AFIP-r2!3259.LX C3H)' females' 25 msrrhs old'
aluiiry chronic roxicity scudy' 1
sacrificed after T;;;;"'"n
Ffiffie
"
b'ere PreParedfrm 12
(In order Eo provide elough sfidei of Che lesion, secCions
The nimber in parenEhesesis Ehe
differenC mice,-o,"rt of wfiicn ".i" "*'arols'
as sporlaneous and noc relared
j.ndividaul anirnl code.) these lesions .," "guidJ
Case III
Eo EreaEEnC.
-
8150-80
CaseIV
---f"c"--..-dffi
'
(AFiP 1757063)
r-unbiaewevrasP:9t"1:-1^:::l"veEerin'-iar
trris e*e and severar ochers in,o
i"r*--puicr,ased
feed and weigha
deprE6ffiff
sbowedsigns of sickness except this one&an
l,linnesoca6 rpnths previously,.*u
coxnrprotein, A, D E'
one orher rhar was iosing wgiglEl'-$;p;;."i"a-rz-14%
Ehere was
aiEa-a nouir'afcer presen.a'ion. AE necropsy
Arl sheep in ary-ror.--fi.
urine and ilark bro'wnkidneys'
generaliz"O i"rlrill'"oTi."-"oio..a
TI}OTHY P. O'NEILL
Ciptain, USAF, 359(VC)
of VeterinarY Pachologl
n"litttv
Oe&.tllEnr
^c ''I:Eerin l-''' D'::hr. I ^-"
Results
tilednesdaySllde Conference - No. 28
13 l,tay l98l
CaseI - n-2L2
pilorides)
ar-old ferrale Cubanhutia (Caproys
was acqufrd fror a zoo in Florida five mnchs earlier and recently F
ancnectic ard ehc'Ircdweight loss.
tnac
Cancributor's Diagnos{e& CsryFncs: Ltng, rrEtastaEichepacocellularcarcirma.
uruEual in chat each toUe is subdivided
lnro ntmrous snaller lobeg, ifrartlrrg a "cubqd" appearanceEo it, There was a
large (5 o) pedurrculatedmaeeinvolving che lefE lobe ae well as qall rpdules in
ocher lobee. Both lungs tnre uore liver-like than lurrg-ltke. The ctmr also
reEastasized to the adrenal and hearc. The arinal also had a sall ovarian
granulosa-ttrecacell. tumr.
Attendeee I
cffiia
ls & Comnte:
carcinma retastaEic to lung, Cr-bryl
is a large (3-5 lb) urivorous rodenEfro
the
Caribbean lelande. There was gqreral 4greeonnEwith che cantrlbucorre diagnoeis.
A1l attendees agreed chat the cellular uorptrology nas cqatlble
with hepatic
tlseue ard rhat hematos,enous
spread lras che couce of rretasEasis. Sue accendees
submitced an equally aEceptabli preconference diagnosis of uerascatic
heparocellular adenocarcinora based on the acinar formacion by che neoplastic
ceils.
Patrerne found in hepacic turnrs other than acinar ari rrabec,rlar and solid
sheecg of trmr cells.
Hepatocellular carclnoae have a high rate of recascasis to
che lung ln rgcs, Mice alao may have retascatic leslons of liver trrrrs.
Contributor:
National Zmloglcal Park, tlashington, E
m008.
Referenceg:
Pathology of Zoo
of turcrs in zoo aninale. IN Cqarative
F-Fffiview
Press,
Institutidl
fuiEh'scrian
Animals, R. J. lbntali and G. t'ttgaki, Ede.,
I^lashingtcr, m, 1980, pp 53f-542.
2.- Hiecologlc Eyplng of llver tLurcrs of che rat. JltrI 64 (1): 180-206, 1980.
nFcory:
Ae
ffiffi Hryhi
m
:xi.i,$.,H:liit#iilffi
Fffidead
:il,'[rtu"
H:rhre"
:F::,"ffyi"tli:i ;::,3}|ff0 on""tir*e;1-A"ir, *a wasror.nd
*.ri
male, ringneck c
w
Inirial
l:,goo/m3
927"
8%
357"
4.8 gn/dl
seg.
Lyryh.
HemacocriE
Tocal protein
I week lacer
IO,SOO/m3
937"
Tl"
30x
4.4 gI/dL
ie&
setione
Avian tuberculosis.
.ssrswere present in very large nrders
in boch
and cissue iuprinre. culrures and serocyping
Eiru in-p"il"il."
"".
&
:Tff;:fr#::Ti!:.Hii
),:i:ffi
i!Ii5ii,LlJl";.llFl"!,,fly,f
,,,*
iifl*;
-ia4$@t
"l,il
i
:
:
I
:Hilti#fr
:
i:
lji
i"
*u*:,
:
ffi
Diagrrosre
: ruueidil;-'(r,y#ffi;iffffiiilri$g,
""
heparicis
and
ffi?"t:ut"
!i", -gF*ulcntous, chronic, uulcifocal,
pneuuuria.
8ffllg; ffi:?iii::il.X;.iffi
prrpo*ar
hepacoce*urar
;i-t* ; it"'in*""roii.ffiirffit:"i33ii:i),itifr,fl*:"ffr"#"iff "
the variable presentacion
,:TiI
oi-mycoua"i"rioriJ-iiuirar. rnLracerlularbacrr.l
,,riEhinmacroplrage'-were
observ;d
- pu;ipfi;;y of rhe lesion* tn
H&r.
"r-it"
i"'
u
r
i'
iili,,ffi i i"**yt"'i''ir -p,che
i,"ri6
f;!!i,31ft
,"o}lTl, Hi:'*gl"
necrosig, granulons ind fibrosis
*r"-Ju"ru"a-by- rhe parricipanrs.
Parasiric cysrs (probablyt;;;
"rtio
cqtcrlbutor:,- Divisign of corparative
-r,E
Medicine, sctrool of lbdicio€,
HopkTfrtlhrvlEtsiicy, gafiitrnre,
ZI2O5.
T?reJohns
Reference
ry-coffirial
rnfections of zoo Animals. IrrnEari, R. J., Ed.,
$nirhsonian
Insticution preee, I,rtashingLon,
m, 197g.
CaseIII
- 80-002 (AFIP U83250).
X C3H), fesnles, 25 spncns old'
years
on a dietary qrronic toxicicy scudy.
2
afier
i""d
l'lice were
"""iif
lesicr, iections were Prepared.fron L/
the
of
slidei
prouiae-enougn
(tn order to
-tt""" were cdlcrols. The nr-sber in parenchesesis cne
diff"r"nt mici, upsr of wfitch
lesions are regarded as sponlaneousand noc relaced
individaul animal-""a".1
to EreattrEnE.
Concributor's Diapnosls & Cmntg:
Fibrous osceodystroPhy(ftbro-osseqrs
lesi
lesiois mied tn'sr'n" slides include: muse (25) - lyryhma; rcuse-(00)
laaici*"I
- epidermid cyst of spinal cord; nice (62) and (86) - hernlated lntervertebraL
disis; rrpuse (f7) axonil degenreracionand denryelinatiqr, sligftc.
Cospnt: Prisenred are"decalclfied sectiorrs of choracic spinal colr.rr with
spinal cord and adiacenr soft cissue.
-'--ifti"-t""ion
is'noced quite frequently in che aged fggale B'6G3FImuse. In cnis
o"i"oayscrriphy was dlagnoied histologically qr I of 96 (1%) concrol
rruai,-iiUroG
and 68 of. 94 QZb' concrol Femalenice. tle trave noted vertebral f ibrous
*i;';ri[
osceodyscrophy in fenale nlce as early as 15 trslths of age.
fn6 p"tholenesis of che leslm ls-mkno{dn. The paraEhyroid glands lYt. __ .
norual. There was frequenEly
hisfologicalf
-lesions.a lor grade chronic renai disease buc
Chronic renal disease of similar
chis wa! noc correlaced wlth the bone
severity was also presenE in che srale mice. AB rePortd !f lass and lbncalir'
ncst of'ctre fesrale'mlce have cyecic endoncrial hyperplasia lendlng further suPPJrE
sex horupnal imbalance.
co posaible
'!,le
h6ve nored lncreased serLsl alkaline phoephataseacElviEy ln che aged female
rDuse. Fearalemlce aE cwo years of age geniraliy have alkaljne_PhoPhacasevalues
4-5 cisEs higher fhan males. In mice under-I year of age,. Ehe tesBles generarry
have alkalhE phoephatasevaluee of. L.5-2, Eiune Ehoseof che male mice.
tfre ievel of alkallne phosphataseappears to have a crule coFr::lation
eaaiti*"ify,
of
the bone leslon.
severity
with
AccendeestDiagnosis & Cdrrents:
l
, redullalY, uru}gl{qg"t co di'ffuse, mild co
rnderatl, nafrow caiicy, vercebrae, spinal columt,,B6c3Fl muse.
CGsEnEs: As notea'by che ccrtriSutor, ocher lesidts btere Presenc in s@
slides. The fibro*osseoui tesions rrere ch; prirnary interesc. A gradacion of
fibroaig in che nutrrow cavities was obeerved. Fibrous clssue replacecenE can ranBe
frmr stild to cGDleEe. The cellular orlgin ie r.rrkrnrrnbuc ls believed to be a
Lesions urcscofcen are fourd in che sEernebrae,
m,rltipocenclal rrirro&r scen cell.
th9 lons bcres. scrain irnidence,
smtires
il;-;E;;;6;;;-"i-rh"-epi*t-coi-m-"nd
sex oredileccior and hbrrsral tnf luence (particularly the tole of -escro.gens)a|e
pathogenisib of the lebions. vicmin and mineral
;h;dh;-6-b.-ilu;i;rd-G-iil
i""oni"ry hyperparathyroidism are not chought co play
;nd;;idi
il;i;;";;
"rOlor
a role io this condiclon.
ContrlbuLor:
Yi61"lill,ffi{ffia'
Toxicology ResearchLaboraEory, The Do'l Chemical Co',
References:
ffi&Montali,R.J.:Spo19qne9us^fibro-os9eous1esiorrsinaging
female nlce. Lab. Anim. Sci. 30: 907-909, 1980'
N"oiia"li" tnd ,ro**plastlc,^k:ign"-i1^"ei.tg
2. Ward, J. M. ec al.,
X C3g/tteNlri tnOcjFl) nice. J.'Nat1. carrcerInst. 63: 849-854, L979'
(C57BL/6N
presented to the vecerinarian
eu"a-*6-i"d;F
q'\' wsrEuLffi;-T":ffi":ff
gurchased
ross.
$:*ffif
this esreand sever.r oit"." ln
t'tiirnesoia'5-rcni:-y
rrsvrvuntrti ;. no.e_gr,oiled
riqre snowed sims.of^sickness
p::y:?"ltf
Jis," or ,ic["GJ except
one
orhcr ,-*..
Ji"".,. rhio
m ^
one other
*ris-onelrd
th;-;"-.lll
Sheep
;";;%d
L!:L!T
LZ_L47"corn/prolein,
Art shean ,_ ,-,]"f^lo.i$
Ei4r,
.
corn/nrn,,ain A,
a o-C
n r i'.
F
--Efr
g:!fo;.
TiS',; dffiP"f:::
3*-:*:l,tn,
generaliied
lcclrus, .oii""-"Ji;jTi":'ff:
Cqrtributor
ta
At^necroisv'ttreie-was
H:l'ilIlllT;
dark
brqm kldneys.
Chraric, -rxnsupFJrat
- lve_choJaqgit
widespread,
I ivli; pdi.t-'ciffiEi", is,-,ilr"r.a,
-,
.jiJil*, necroeis arrdearly-regeneraai;,
f 1;"l*:i,,iiy31
_f"iilri.'"iJft
with_nurerousheu;gdbi;-"#3:
g'" 9*- iindinss
- ^SIpo"
._
rira,"i,
;#,nor'uppuritive
ff#tril#""#;"5ilffi
;J'ff*i";f
cause of the îli.H"iiir:Ti:iT""i$
ctroiangitis ci,ira *t u" dereroined.
:H::'
rry-sv'rrr6.
Lrver copper revel-yn1.
?90 ppg_wEt wetgtri latoic
':--r4u-r,6'r ur.gnoseS:
_ r) crn-railgitis, chrqdc, diffuse,
uottsran
mderate, liver,
eete'
ov{ne'
2)
Flbioef,
3) proliferaricr,uiiiari,îiiF"*, niiariiil.
dit*iti-i,-"trg"_i-",_aiti;,
@derate,river.
Edfi;iJil,
u"f;
diffuse,mild,
.zt re,
;F.$:'f*#:';*.'"
k
i
dney
'rqtyrrtrpc*r:
.
" ;'oiJul;'i
H$;;ii6";iij"
tJrver _ toxic hepatitis
Etiology: coppermxrli$sl
u@nt':
- n*tlobinuric nephrosis.
there was general
agreeuent with the contributorra
couents' Additionallv minv
diagnoses ard
oegenerationandnecroiis # ;-d-pti;idi""aiiert*r,
hepatocelrurar
"itentg"g "il"ii"."a-""r,ilii;;i;"
coppei-roxicosiswasrhe
diagnosis.baeed-m-inl
PlParv
i,irtorv rnd hisEorogi""r-i55i*l' in
KI.n'"v' A rhodamlne
rrre river ard
s,"in f*
confinedro rhe cenrrirobiir"r-tit*tocyres
:9qp:r ras poJiii""l- nr" #;ial
stained wae
ila-irn*oidal,a"ioft"gu".
attendeesalso oroposed
Ttre
a-con"uti"nt
iir"ui'-"indirion
cholargitie and'bilj?{t;";ifi;"rion,-rrJili"il"n
,"rpo*lLre for rhe
eriorogywasnor determined.
Ili,li"iffi,.i:,'::"HlfiU*l;S*i#Tj.::*".
*orffi
roxic
acrion
ortr"meiouin
orveter
inary
,ffT[H::"lf ,:"I;iffi II,rffi*]i51i.couege
Fefe,rences:
r' G@neratne' s' R' et a1':'
fluid of copper poiloryd
-copper, zinc ry{ itgl levels in che cerebrospinal
-R: ;il;:
n"s.'vei.-a;i.
2723{14_385,Lg7g.
2' c@rGiaht, S'
;;;I::
creatine
[ii""".rerease
and m'scre chanses in
chronic copper spoiearing-i"-;h;p.
Res. v.[. sc i. 2g: 351_361, 19g0.
King,r. p.
.3.
iff:;fftili1,
eEarl: a"l.ptigl; il.orisis i' riuo-jJiiag
tr,.
phaee
of crrronic-"opp."
poisofii;!in sheep.r. cq. parh.
8e:
4' Gooneratne'
An ultrastructursl ard-mrphreEric study
-s' R' et al.l
liver of nornar
prctor. 99 (2): 42g_t*g, of the
"na "opp"":pd;;""d "il;.--A*i.
r9s0.
TII'OTHYP. O'I'TEII..L
Caprain, USAF,8SC(VC)
Registry of Veterinary eatholoev
Departrrentof r/erer iniry p"rho%ey
llistories
AFIP Wednesday
20 l'lay 1981
Case I - 032-02581
P L6L972L
uDuse rece ved I0 ppb diethylscilbestrol
6 weeks of age until sacrifice 559 days later.
laboratorv Results:
@tely
in the groin.
Case II
in che feed
hard urass, abouE15 m in maxi.u.mdiamecer, hrasfotnrd
- IAIR 26035 (AFIP U58835).
old fenale Gordon Seccer that had a history of
progffiE-cl$
When running and going uP sEePs she exhibited marked
dysfunction.
and hyperextension of frqrt legs.- She was acEive,, alert, respctsive
hyperretria
The dog was eutbanized sirpe her cqrditicr
ahi otherwise in appatent good health.
a bieedirg, humcing, or sfrow animal.
made her msuitable-as
Case III
- A14-1593-80
P u58461
dld l.lLrite Pekin duck with historY of leg
30%rprLality in $oyp of 2,0Q. Gross lesicrs htere seen as swollen
Livers and spleens wiEh occasimal subcapsular hercrrhages.
Case IV - VRLA-81891 (AnP L776777)SectTolF6l-several
Eh feEus' Tne Piacenta was thickened.
tissues, includirg ltrng,and placenta, were submitted.
TI}'OftT{ P. O'NEILL
Reiiscry of VeterinarY PathologY
Oeftrtu6nt of Veterinary Pachology
Resulcs
AFIP l,lednesday Slide Conference - No. 29
20 May l98t
Case I - 032-02\8f (AFIF L6L972L)
d t0 ppb diechylstilbescrol
beginn-inEfr 6 weeks of age LrnEii sacrifiib 559 days lacer.
Laboracory Resulcs:
@tely
in Ehe groin.
#
Cqrcributor's Di
in the feed
hard mass, about 15 um in maxirmrn dixrpcer,
was fotmd
is & Cmrts:
I.{alignant granular cell ulrcblastoa.
granu
turr:rs wera firsE thot€ht to be of mrscle
but s6E chink they are derived
ifferenEiatd resernhynal cells (4).
man, they have been observed in scriated muscle, particularly of Ehe tctgue, but
can occur alupsE anynrtrere. The commn variecy consists of many large cells wich
small nuclei and a celL body mlded by pressure of its neighbors into varying
shapes. Ttre cycoplasm contains diastase resistant PASpositive granules. lhe
malignanc variecy ie variously called malignanc orgaroid granular cell nryoblastma,
maligrrant nonchrcnaffin paraganglima and alveolar soft part sarcqna. They occur
chiefly in mrscles and ofcen retastasize chrough the blood streEm. They have an
organoid pattern with surall rounded groups of cells surrouded by a fibrovascular
frangwork. ltre nucler are relatively larger than in benign ct.uDrs and have mre
prorinent nucleoli.
Their exact nature and relation to the benign ttmrg is
rmcertain. tilqrhuurangranular cell currcrs are rare and have been indr.rcedin che
ucerine cervix of mice by estrogen creatuEnt (1).
Actendees' Djagrosis & Cqre.c"r
c3H
Granular cell tr-spr (nryoblascon), cervix,
Ccsuencs: Tbere !{as general agremnc wich the contributor's diagnosis.
Several actendees also preferred a malignant designation for the tumr. This tLmr
has been repoEtd in a iariety of specids (3). These ctnnrs occur msc often in
the oral cavtcy of hrmans, the lung-of horses, the tongue of gogq srd che brain of
raEs. There aie also reporEs of uhese twDrs in che cervix of mice EreaEedwith
escrogens (1,2). One reiort describes chis tumr in che cerebru o.f a dog (3). As
uhe cdntrlbutor has poinled ouc the specific cell of origin ie not knotrn, but mst
pachologists favor a'Schwanncell ori!,in based on several histological
ulcrastiuctural feacures (3,4). Cells termed angulate bodies also have been
observed in these cutDrs (3) and are besc seen wich the PASreaction ac the
puiipt"ry of rhe lobules.- these turors should not be confused with nonchrmaffin
parasanqlioras as there are several histologic dif ferences bettreen the cHo (5).
tte EmJr also rusr be differenciaced frm nisciocytic inflamatory reaccig.": . Tne
in Ehe producrion of chese ttmrs in mice has not been elucidated.
iol" of
""iiog"n"
ConEribucor: Na6ional Center for Toxicological Research, Pachology-Services
proje
ity of Arkansas for I'bdical Sclence, Jefferson, AR 72074-
References:
WB.etal..:9y'::9f:|"epididyuris,cancerofthecervix,granuIar
cerl nryoblastcnu, and other resrons atEer estrogen
-----(
injeccion-in newbornmice.
J . N a c ' I . Ca n ce rIn sE . 3 t: 4 2 5 -455,Lg65.
tteopra6ci.-"nA preneoplasric lesions induced in female
r Hig@r.r, B. et al.:
c3H.urice^bydiets concaining DiichylscirbesEior or l7B-Escradiol.
veeereY^vr
J. Envirqr.
Pathol.&Toxicol.4:81-95,-1990.'
3. Parker,G. A- er ar.: cerebrlr gr??u!arcelr,F1.ml
-6g-(Al, (r,tyoblascqa)
in a dog:
case.Reqol-and
LirerarureReview.corilell tal.
iod_iZO,Lg7g.
-apptt-L:c"r,r,rry-cioir",
- .4t sobel, H, J. et al.: Granular cells
celr resions. rn
Pachology
annual, somers, s. E. qq.), rF "oa-gr"nular
Pren_ciceHall, Newy9rk, L974, pp-43_i9.
TtuDrs of -.'- .5-' SEout, A. P. & Lattes,'R.i
the sofc tissues.
Pachology, AFIP, Idash. E, Lg6l , pp fAO_fa6:
Aclas ef frrrnl
P 1758835
Ld female Gordon SeEEer that had a hiscory of
ap_parent.
gq.heal.ch.
the dog was euchanized
nradeher unsuirabri'as a bieeding,-tt*cirg,
"in""-her
oi""tow anirnal.
tqrdiricr
Qsnqlibutorrs Diagnosis & Ccrencs:
ical abiocrophy of Gordonsetters.
lbrpholoeic Diagnoses: f)
ryg$erarion *a necrb"i., piogiessive, rulrifocal
co diffuse, FurkinjE ceus, c6reu5ti^,
2) Arrophy, diffuse, rcderare,
granular cell layer, cerebelltm.
"ri*.
Couencs: 0ver
lle po"q 9 years vecerinarians in Newyork, pennsylvania,
Virginia, l9!th carolina'and
caiifornia tt"ve-ouserved a Eoral of
dogs wit:tr clinical histories c,onsisrent wich a-stcnty pro[i"rriue iA Eiriaon-rim..
cerebellar
disease. Necropsies of ten of these aog"-ilv. revealed siuritar
cerebellar cortical
degeneracive leiions.
The clinical sffi
qrriet
are Ehose
cerebellar corcical degeneration priuraiity "nA-f"rions putinje-ce[s.of lare
The
rerm
abio.trgghy has been appriea to ch; degeneiation
"ir""cing
of a cell due ro- ssrF ir..insic
sEcabolic abnormality- thac causes prdature aeattr of char
rn chis disease,
Purkinje cells form norually and apparencly funcricr for a""ir.
nr-der of upnths orior
to-cheir degeneracion and ohser of irinicai signs. Examinariqr'or-p"li*;;.t;i"
atrecEed do-gssuggests that che abnormality mat be geneEically deceiminA. lbre
animals attd pedigiees must be scudied befoie c['t. ooE
is
determined. Similar lesions have been observed in other
"i-i"n""icance
breeds such as Kerry
brue Eerriers and rough coaced collies frqr Australia.
Accendees'Di
c
ation ard necrosis, 'CorJsr
neuronal, mrlcifocal co
mderate^to
severe,
cells, cerebelfm,
setrer, canine.
fif
fy:",,
-Purklnje,
2) Atrophy, mrlcifocal co diffuse, frita to rnderace, granular cells, ..reu.riu,.
Hereditary cerebellar cortilal
abiocrophy of Gordon
. EEiologic Diagmsis:
seECers.
Eciology: Aucosonl recessive EraiE.
Cqnrencs: There was unaniirDus agreerrEnEwich the contributor's
diagrnsis and
co0rlEnEs. Sme variaclon in the dqg,ree of Purkinje cell loss and granular cell
atrophy existed aslcng the submicced"slides. fne itinical
and hisrdlogi".f
iu"cur""
of che condicion. have been published recenrly (4). The neuropachological
changes
are reported co be confined'to che cereb"ttd,, ' 6;"";tt
ln!-#i.o"iiffi-r.y
reduced in weighE and the folia are chinned. The hiscorogicar
changesreporced are
similar to those observed in chis case. There are other Inherited;;.;lffi;;l
condiciqrs in several breeds of dogs wich-sone of che
rrisiopaEhological
features of rhis condirion in Gord6n;a;.;;-lt,i,j,i).
""ue
cqttributor:
sanFGi@Ta
References:
TCEffi'ua,
Letceraan Arny
' Instituce of Research, sGRD-uLV-p,presidio of
94uzg
A. et al.:
Hereditary
cerebellar
cortical
and
nuclêar,abiotrophy in Kerry brue rerrier's. .latnA r6a: 1n9-1124 extrapyramidal
, Lg76.
z' Qe Lahunta, A.: Veterinary Neuroanacouy
--------'r and Clinical Neuiolog5l.
W. B: Saurders, philadelphia, Lgli.
3. de Lahurta, A.:- Coparative cerebellar diseases in drestic animals.
Coap. Vet. Ed. II: 8-I9, 1980.
4. de Lahunta, A. et al.:
Cerebellar cortical abiotrophy in the Gordar
setter. JA\'MA177: 538-54I,1990
5. flartley, W. J. et al.:
Intrerited cerebellar degeneration in the rotgh
coated collie. Ausrr. Vet. pracr, L-7, Lg7g.
caseIII - eb93:gQ_l4[rp u58461).
d-Whicepekin duck wirn hiscory of leg
weakiFffil
30%uprtiliiy-i"-gtoup
of 2,000. Gross lesions !{ere seen as srorlerr
rivers and spleens with occasiSnal' subcapsurar herprrhages
Laboratory Resulcs:
chem*_-'
Erysipelothrix
insidiosa
was isolated
frqr
the livers
of
Gqrtributor's
Diagnosis & Ccrrrencs: Erysipelas.
lre;-[;prric
congescicr, chronic
pericholangiEiq-and RE-ceIl swelling, with cyroplasmic vacuoles, rnany
of wtrich
contain basophilic sraining rd snafed bacc6rii.
A t r e n d e e s'D
'.. -rcqpn9ro81cDiagnoses:_!) Chotangitis, chronic, diffuse, mild, liver, I,lhite
l.!i" duck, avian. 2) Proliferation 6nd swelling, sinusoidai fining cetfi,'.----diffuse, mild eo nnderace, liverr 3) Bacceremiai'difrus", ,ild, liier
4) Vacr.rolarchange, diffuse, mild, liver.
Etiologic Diagnoses: Bacrerial chorangicis and heparicis.
Eriorogy: Erysipelo-rT|fii:t:H$:
(vacrrclar)secondarv
co hepaticcqrsestiqr'
Couents: TnTeIfETon-_to Effi
diagrnses,-The
a mrphologic diagnosis of
hepatocellular necrosis also was giverr by a-few.
majorit| of thE accerdees
submitted a preconference etiologic diagnosis of Lankestirelti sp. or orher
Protozoa.Attendeesfelctherewaspar-sitismoffiIeukocytic
Procozoal etiology was disirediced based on several factors, incilding,
??ll":.
rack of {dernnsErable schizo-nts, plemnrphism of the organisms ard free organiss
wichin vessels and sinusoids. Miny of ihe basophilic intracycoplnsnic scilrctures
surrotrnde{ !y cfe.ar spaces were
-interpreted as stages of eryi:hrbpnagocytosis, whictr
could be followed sequentially fron
whole Rff,'s co*pyknotic"nuclear-foims.
NuuErousGramposiEive coccobacilri were presenc
in RE cells and vascular spaces.
Amixed baccerial and protoio"I inr"cr;o,,';;;lJ
noc
be ruled ouc corprecely. The
gross and hiscologic risions of erysipel.;rs
in-uiros rrr" i"ui"*"d.
Turkeys are che
species rcst cmrcnly affected wich ri,, ,li,raielc
eco,ncrntclosses occurring in chese
birds.
hI.".ffi**iffi,liseaseDiaqnrlr;f-i.::Laboratory,Purdr.pUniversicy,
Reference:
@A.A.eta1.:Patholog1rr:{'41periuEnca1erysipe1asinturkeys.
Avian Dis. 22: 503-518, Lg7g.
CaseIV - VRLe:lllggl_Gerp
L776777).
th fetus. fhe placenta was thickened.
SectTdTAFseveral tissues, inchraing 1.,-^ -rr
rlacenta, were suhicced.
cqrtributor's Diaspsis & c@gqts: coccidioidcrycosis,
fetal pneuwria and
plac
cments:
The mare was frot the Nevadaarea.
location are unusu€rr.-Maly-aire"."nc'ir"g"r;iowm The nr-nrberof organisns and che
scagescan be identified in
ncsc of che slides. speciil stains were not necessary.
uragnoses: r) Pracentitis, necrogi?ing,subacute,diffuse, gevere,
- r''r'LPtrJrugrc
placenta, fetus, eq-ine.inieistiti"r,
-2) Fner-un-nia,
subacuceco
Ei"nur*tou.,
chronic,-rulcifocai ro diffuse,
.roaqiai";--lG.
Etiologic Diagnosis: coccidi.;ii."ri;ciI'ii""""ciris
and pner-unnia.
r - --- r-'
.
Etiology: coccidioides
inmitis.
csuents : TffililEFliianiirDiq-ag.
reetrEnEwi rh rhe concr ibucor, s eEiologic
diagnosis and cqmenEs. sme variation
--sm;-;;IJna.es
in rhe preconference mrphologic
diagnoses for rhe placenta ana run[.
"*irt"o
preferred pyogranurura.ousor
granulonLous placencitis and pyggianulunEou"
-Th;-il;gatoi ;;fi1;ioJ"'brornhopneuuria
due to the variacion in cheir 5iii.".
organisms (spherures and
e.ndospores)were readily seen. Cio"s l;;i";;';ssociared
wirri ttris form of rhe
disease have been repo:Led (L,2>-. The rouce of fungal
io ,t. Luqg frm rhe
placenta was discussid. ucic'o't-*,."p";;;;;.s
"p""rJ
favoreil
an
inharacion
rouEeove'
a hemacogenous
spread as resions i" o[r,"" o;;;;; $rerenoc described.
concributor:- vecerinary RefererrceLaboracory, p. o. Box 30633,
SaIc Lake Cicy,
utah:mrr-
References:
& Hunc. Vecerinary pachology, 4ch Ed., Lea & Febiger,
_ "T5ffi-Jones
Philadelphia,
L972, pp 646-&9.
_ _ z . - L a n g h a mR, . F . - e c a l . : A b o r t i o n i n a m a r edue to coccidioidurycosis.
JAWIA170: 178-180, L97t.
rI}{f,II{Y P. O'NEILL
Regiscry of Vererinary pathology
Departnencof Vecerinary pachofogy
4
Histories
27 May L98L
Caee 1 -
P 1777000
Eerhorse stsre with slowly developing cutaneous nodules
CaseII - 80-83 (AnP I783r94).
for E'iffif
previously.
a 3-year-old, female Bedlington terrier presenced
lethargy, anorexia, voition,
and icterus. She 6ad whelped 3'rceks
(2 microslides, H&Eand rhodanine).
Laboratory Results:
@.rsddl
Alburin
oc-l globulin
o<-2 globulin
,6-globulin
7 -globulin
A/G ratio
Case III
%.8%
2.8%
24.8"L
23.8%
L4.2%
0.5
BI.]N
Alk Phos
LU{
SG T
SGPT
Toc bilirubin
Direct rl
Glucose (fasEing)
V+ ryldL
510 ru/rnt
660 mu/ml
830 mr/nl
3440 mu/mt
r0.9 ry/dl
r0.0 ry/dl
f49 ry,/df
- I.It 80-889 (AFIP L75748L).
d male cac was presenEed for anorexia and pale
---.4
mucous merrbranes. Hematology revealed a non-regenerative anemia and atypical
A
Iyrptocytes with dark blue cytoplasm. PlaEelets levels were about 2 million/mJ.
At necropsy Ehere ltas generalized lyrph node enlargerent, an enlarged mahoganyred
spleen and a pale tan liver containiqg disseninated pin-point yellor foci. There
was also mild icLerus.
CaseIV - BG-81(AFIP U83f98).
tion represencs a lesion found during necropsy of
an adulFWhTEd-Carneaupigeon frcm the block at our research farm used for
atherosclerosis research. T\n organs' saples are present.
TII4TI}T{P. O,NEILL
Regisury of Veterinary PachologY
Departrrenc of Veterinary Pacholog5l
Results
AFIP l{ednesdaySllde Conference- N o . f 0
27 May LSBI
Case I - ffi4t4
Fttstorv:
and progresslve emaclatlon.
rhorsemarewithsIow1ydeve1opingcutaneousnoc|u1es
Contributo
:T:*iI*.i:.Ili:"iill3
T:::.,Tiorm
rn
appearance.Theywerenot foundrn otner-rocationJ
i;rdiilvrscera
andregronar
tvmphnodes. TwirpopuralionJ-orceris
i;
il;-i;;i;";';;;"firphocytes
pleomorphlc
andrarse
retrcuia; ;il;.gryro*trriE
sp. wasisorat6dfrom,,trmori
tissue. IgGlevels in inJ-ier.um
werenormal.
Attendeest
- . p h o e y t i c 1 y r t p h o s a r c o m a ' s k i n , Q u a r t e r h o r s e ,
equine.
comments: There was general agreement
wlth_the contrlbutor,s dlagnosls and
comments' Thls-case crosiry pararieis
.
t",i!nhy
reported case
-olner
of cutaneous
Iymphosarccrna
in a horsewrtr,r6ut
vrscJrar-iiJoiu"rnt (r).
casesof equlne
cutaneouslymphosarcoma
wereaccompanied
by viscerat lnvorvementand mayrepresent
rdter stageg pf the samediseaie. 'severar'atlenoeeJ-i"i""iJJlntracytoprasmle,
linear, and crvstalllne rormi-;ltgl_li;tiqffi.
U n k n o w n b u t m a y b e r e 1 a t e d t o d e g e n e r a t 1 n celrs.
g c o ' v @rheir
s p . bsisniflcance
a c t e r i a 1 o r gisa n i s m s
( t)' The isolation of bacterii-ffi-;i;;;"rffitn"
tumorsug@stsa defectlve
immune
response
!l).-of lil;t;-inJiJ"["-c;ry;#dr-uJ"iriii"lEiirr"te
macrophases
anc'enhanceorowth
tumors. Thls
that in additlon to the
multicentrtc] thvmrcano'iii,i""::ll_io-iil"-oi"iimpr,osarcqna,
"..r-iilusirates
a form
thai is prtmarily cutaneoJi-in"n"cu"".
Contributor:
exists in horses
National Animal DiseaseCenter, Ames,
IA.
Reference:
sneanan' B' J' et al':
Hlstlolymphocytic
--, --- lymphosarcoma
')
ln the subcutis of two
norses. Vet. path. ]7: IZ}_I33,
I9fJ0.
-
CaseII - 80-81(AFIP1783194).
m a 3-year-old, female Bedlington terrier presentecl
--F---4-
for sfgns of lethargy, anorexia, vomition, and lcterus.
previously. (2 mieroslifles, H&Eand rhodamine).
^
.
,
She had whelped f weeks
Laborator
Tot-Frutufn
-1 globulln
-2 globulln
-gIobulln
-globulin
A/G ratto
8.I gm/dl
14.8%
2.8X
24,8X
21.8%
14.2%
0.5
BUN
AIk Phos
Lttl
SG0T
SGPT
Tot blllrubin
Dlrect 'r
Glucose (fasting)
84 mg/d}
510 mr,/ml
660 mu,/ml
830 mu/ml
5440 rnu/ml
I0.9 mg/dl
I0.0 mg/dl
La9 rq/d\
Contributor's Dlaqnosls & Cmmentg; Chronlc-actlve hepatltls, with blle stasls
anO
ln hepatocytes; coinpatibt6 with inherited
copper dlsease of Bedlirgton telrlers.
Cormentsr Grossly the llver was dark green, whlch is explalred by tfe severe
bile stasis evident micmscoplcally. Copper analysis of the liver shored l4O5
micrograms/gn. (wet tlssue). The rhodanine stain demmstrates heavy accrnulatlons
of copper-containirq grarules withln fiepatocytes, prircipalty in the centrllobular
regions. Examlnatlon of other tlssues revealed heavy accrmulatlons of hemosiderin
wlthln the spleen and tubular degeneratlon ln the ktdney.
AttendeesI
Corments:
, centrllobular, chronic-actlve, dlffuse'
moderate, llver.
terrler, canlne. 2) Cholestasls,diffuse, severe,
liver.
Etiologlc Diagnoslsr Inherited copper dlsease of Bedlington terriers.
Etiology: Autosomalrecesslve gene.
Conments: The history lncorrectly states the spectal staln ls a rhpdamlne
stain; thls should be a 'rrhodaninettstain. There was unanimousagreementwith the
contributorrs dlagnosls and qunments. Subacuteto chronlc cholangitis also was
included ln the diagnoses llsted by sone attendees. Values for the following
clinlca] pathology parameterswere elevated: total proteln, alpha I&2 proteins'
Serumalbmln and the
BIJN,SAP,Lfr, SGOT,SGPTand total and dlrect bllirubln.
A/G ratio were low. Hepatlc hemoslderoslsalso was present in this dog.
Hemoslderosisusually ls present ln Bedlington llvers and often ln large arounts.
This disease
Lesions ln other organs have been cormentedon by the contributor.
has been eomparedto l{llsonrs dlsease of man. Several dlssimi}arlties betweenthe
condition ln manand anlmals do exist.
Contributor: Dlvislon of ConparativePathology, ArmedForces Radiobiology
ReseEF6h-Tffifffite, NN|€, Bethesda, E 20014.
References:
The liver ln the inherited copper disease of Bedlingtm
f. imwrtg, J. et al.l
terriers. Lab. Invest. 43 9L): 82-87, 1980.
2. Twedt, D. C. et al.: Cllnlcal, morphologlc, and chemleal studles on copper
JAVMAI75: 269-275, L979.
toxlcosis of Bedllngton terriers.
stalns
and the syndroneof primary billary
J.
et
al.:
Copper
3. Ludwlg,
cirrhosis: Evaluation of staining methodsand thelr usefulness for diagnosis and
trials of penlcillamlne treatment. Arch Pathol Lab Med10f:' 467, L979.
Case III
-,-ycar-OJ.O
year
mucous
CaS
for.anorexiaand pale
fiil;d#!îiii'
o"i:fiii:iiriii"ir.F,li!ilisg:ll*:"ffi,i;*1f,.ffi;
ion/nrnl.
::i::,3.'#ffi
:{*;
$'::i":ffi
t
s.
Hematoloov
::]:
:*
:::-tT::nted
itrffijF:j:;:;iilt':?!ffitrfi:iii,ff.
T:i:
coniainin!
oi.J",ii,iiiH"iliL:;"il."liii8iiffiif"iil"i:
;:*":lJ"ffr?rrilillan.Iiver
lcterus.
At
neCfOOSv
there
brac rrahana'r{-^r
r.---L
ffi'{i?3iiifil
::[,:':]oilii",r,-type
ce,,s
mesakarvocvtes.
[U:i::;#f
t:::,).*,::1,^*
ii::î:iii
]ii!_i#Jiff::"Jldi.$,',S1t3"
centra]
n"oiroei::rl
;pS:ilif,?;
ir_l;il.;ilF::T"lTi:i
erther
or paracentrar rn locatlon
::?:::it^:l*:l-tr
rocetlon nrsn
nnaaaas
also ar..
are-present.
iyfrI'rH;T; i#;:i';il,'*:l'3f
SliSili,Bi;'?lFi?i"
extenslvelv
:i:^'*.::ii^
inftltrateo-oi
celi'-iil;
ffi;'d#Jfli;"tr:Tir3i:*i#"#,,:l: wasnot
tested for FeLV.
""nii".
The.
ytold.let*emla, llver, fellne.
'5ffi*;:,."ig,ijdl!l;l;.ifTi"i$liytfu';:,,1{ili3,,'frH
,,ver.
";peFHill:i"#T:"ni,:ili:";g::*n:,ifr-:;!i':::"H$:i
qiiJj":i;:
-Ih;liili-or"gno"r"
'h,'"I!"r
i:$lf*?:^ "Ho.::s_present;
*l]::#lSiiiir.
1;":""ffi5i[i:1.i"_1;1,;?:
li:".,f,i1T9":
iiiffi"g'fi
;;,
"|ffi
i::iilelii"
iffi:i.
,l.Ho',;;i:1,:i*Ill
:dl"il'llTJih.'?ii.
i::_fl
ji iff"il'ff",l,1;
;:to:
;ie
iiq'
"1
;ifi
iilil"ll"
:lifl
i.
i;:
rhe
9;
HHIi:"fl
:::::?
hepatic-ieciili'"'ii""F"fi1;T:'3":illJlt;:lllt:f,ld:
"
anemia.
:l:fri::.
ffi!ll
of Pathoblolosv,
unlversityof Temessee,
p. 0. Box
,R"oiilSint
torrffiuffi
Rererences:
,or,ffi"BrT'
2' Jarrett'
L' et al.: Megakarvocvtic
mverosis
in a cat. JAVMA
168(11):
hf'
F' H' et e1.:
-Neoplastic diseases of the haematopoieticand
tyrnphoid
ttssrrs. Butt wfoiitn org
sol ir_zl Ig74.
M'A' et al.i- t{edkaivo"vti"'re,*"rr.
in a dos. Vet. parh. t5:
,rr-l;rloiff!:",
-
:
CaseIV -
AFIP ]78'198
representsa leslm fotnd during necmpsyof a,r
sG[E,SecEIon
aduL
Carneaupigem from the block at our research farm used for
atherosclerosis research. Twoorgans' samplesare present.
Contributor,@Semincrnaofthetestic1ewithmetastasis
to tFffifiEFCorrnents: Two other such cases are present in the files at Bowman-Gray.
Attendees' Diaonosls & Corments:
,test1c1ewithmetastasistothel1ver,I{hite
avlan.
Carneaupigeon,-Dlfferentlal
preconference diagnoses also included malignant
Commbnts:
endocrlne tumors of varlous origln. This tumor althotigh rare has been encountered
ln blrds occurs
A hlgher lncidence of seminomas
in several specles of birds (frt).
in the Btdgerigars (2).
Qqntributoli
Medical School'
Departmenrtof Comparatirc l,lediclne, Bqnman-Gray
wlnsT6frlSEfEfr,-ft.
References:
J. G"; Someurusual gonadal tumors of the fowl. Brit. J. Cancer
mEeTI,
5 t 6 9 , 1 95 1 .
in Dlseasesof Cageand Aviary Birds'
Z. petrak, M. L. et aI.l Neoplasms
I ea & Febiger, Phlladelphia, L969, pp 482-483.
TII'0THYP. 0TNEILL
Captaln, USAF,BSC(VC)
Reglstry of Veterinary PathologY
Defartment of Veterinary Pathologv

1980-1981 - askjpc.org

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