3M™ Petrifilm™ Environmental Listeria Plates

3M™ Petrifilm™ Environmental Listeria Plates
Zero In On Listeria Within Your Facility
• Proven - AOAC –RI Validated
• Flexable test format - Quantitative , SemiQuantitative & Qualitative results
Fast, Easy and
More Informative
Sample using
swab or sponge.
• Fast time to results in 27 – 31 hours 3M Petrifilm
Environmental
Plates
• Prevents
cross contaminationListeria
within your facility
TM
TM
as it requires no enrichment or culture transfers
More informative
than step
presence/absence.
justresults
a repair recovery
.
The 3MTM PetrifilmTM Environmental Listeria Plate provides you with the
• Detects multiple Listeria species – Listeria
information to take the appropriate action.
innocua , Listeria welshimeri & Listeria
monocytogenes
Example:
• Simple
test procedure
- less steps
Sample
A
Samplethan
B
traditional methods
Add buffered
peptone water.
Wait one hour.
Sample
• Ease to use & Interpret
The 3M Petrifilm
Environmental
Listeria Plate
TM
Inoculate and
incubate for
26–30 hours.
TM
Traditional Presence/
Absence Test
(-)
(+)
(+)
Read and
Interpret.
By using the quantitative results from 3M Petrifilm Environmental Listeria Plates as pa
your overall control plan against Listeria, you can know exactly where to target your acti
to best detect, manage and prevent Listeria in your environment.
For more information visit us at
www.3M.com/microbiology or call 1-800-328-6553
Ordering Information
Description
Application
Petrifilm Environmental Listeria
PlatesEnvironmental
ForListeria
detection
of Listeria
3M6447
Petrifilm
Plates
50pcs in environmental samples
3M6498
Listeria Petrifilm spreader 2pcs
Buffered Peptone Water, 200mL each
For repair of Listeria in environmental samples
3M6432
Quick Swabs 50pcs
Large Square Spreader
For use with the Petrifilm Environmental Listeria Plate
Price Cat. No
Conten
$285.00 6447
$32.40 6448
6449
$124.00
6498
50 Plate
200 Pla
20 Bott
2 Sprea
For customer service, call 1300-735-292
To fax an order, use 1800- 067- 639
Visit us online: www.thermofisher.com.au
3
Microbiology
Offer expiry 31/9/10
Item
Product
3M Europe
©2010 Thermo Fisher Scientific Inc. All rights reserved. A.B.N. 52 058 390 917
3M AsiaPacific
3M Australia/New Zealan
Petrifilm
Petrifilm
Petrifilm
2 Environmental
3 Listeria Plates
13M™ Petrifilm™
Zero In On Listeria Within Your Facility
Store unopened pouches at ≤8°C
(≤46°F). Use before expiration date
on package. In areas of high humidity,
it is best to allow pouches to reach
room temperature before opening.
To prevent exposure to moisture,
do not refrigerate opened pouches.
Store resealed pouches in a cool, dry
place for no longer than one month.
Avoid exposing plates to temperature
>25°C (>77°F) and/or the relative
humidity is >50%.
To seal opened pouch, fold end over
and tape shut.
Sample Preparation
3
3
4
5
Collect environmental samples using a
3MTM Quick Swab or equivalent, sponge
or other moistened collection device.
The moistening agent should be
≤10 mL sterile water, buffered peptone
water (BPW) or if sanitizers are present,
neutralizing buffer such as Letheen Broth
or Neutralizing Broth is recommended.
6
Aseptically add 2 mL (swab) or
5 mL (sponge) sterile 20°C–30°C
(68°F – 86°F) buffered peptone water
(repair broth) to the collected sample.
Do not use enrichment broth
on this plate.
Interpretation
Interpretation
Incubation
Incubation
Inoculation
Vigorously mix, stomach or vortex
the collected sample with BPW for
approximately one minute. Allow the
sample to remain at room temperature,
20°C–30°C (68°F–86°F), for 1 hour up
to a maximum of 1.5 hours, then
vigorously mix again. This step is
required for repair of injured Listeria.
<70¡F
<70¡F
10
10
7
12
12
9
11
11
8
Gently place the plastic spreader on the
top film over the inoculum. Do not press,
Gently
the spreader.
plastic spreader
on the
twist
orplace
slide the
Lift spreader.
top
film
over
the
inoculum.
notlevel
press,
Wait
at3M
least
10 minutes
toDo
permit
the
Place
Petrifilm
EL Plate
on
twist
slide
the
Lift spreader.
gel
toorform.
if the inoculum
surface.
LiftNote:
top spreader.
film.
Wait
at
least
10
minutes
to
permit
the
self-spreads, the spreader is not necessary.
gel to form. Note: if the inoculum
self-spreads, the spreader is not necessary.
Incubate plates with clear side up in
stacks of up to 10 for 28 h ± 2 h
Incubate
sideDoupnot
in
at 35°C ±plates
1°C orwith
37°Cclear
± 1°C.
stacks
uphours.
to 10 Incubation
for
28 h ± or
2 beyond
h
With
3M™of30
Electronic
Pipettor
exceed
at
±pipettor
1°C or 37°C
± 1°C.
not
equivalent
held
perpendicular
the35°C
recommended
time
mayDo
yield
exceed
30 hours.
Incubation
to ambiguous
3M Petrifilm
EL Plate,
place 3beyond
mL of
results.
the recommended
time
may yield
sample
onto the center
of bottom
film.
ambiguous results.
3M Petrifilm EL Plates can be counted
or interpreted using a standard colony
3M
Petrifilm
EL Plates
can bemagnifier.
counted
counter
or other
illuminated
or
using
colony
Roll
the
film
downa standard
onto
sample.
Dointerpreted
not top
count
colonies
on thethe
foam
dam
counter
or are
other
illuminated
since
they
removed
from magnifier.
the
Do not count
colonies
onmedium.
the foam dam
selective
influence
of the
since they are removed from the
selective influence of the medium.
The 3M™ Petrifilm™ Environmental Listeria Plate method can be used as a quantitative, semi-quantitative or qualitative test.
The 3M™ Petrifilm™ Environmental Listeria Plate method can be used as a quantitative, semi-quantitative or qualitative test.
13
13
For a quantitative test, count and
record all intense red-violet colonies.
For a quantitative test, count and
You
may
to choose
a quantitative
record
all wish
intense
red-violet
colonies.
test if you take different actions based
You
may
wish
to
choose
a
quantitative
upon the number present.
test if you take different actions based
upon the number present.
Listeria colonies on
this plate: 16
Listeria colonies on
Please refer to the
this"Quantitative
plate: 16 Sampling"
section of this guide for calculating the
Please refer
to theper
"Quantitative
Sampling"
environmental
sample.
quantity
of Listeria
section of this guide for calculating the
quantity of Listeria per environmental sample.
16
16
Optional
Optional
Colonies
may be isolated for further
identication. Lift top lm and pick
Colonies
isolated
the colonymay
frombethe
gel. for further
identication. Lift top lm and pick
the colony from the gel.
14
14
For a semi-quantitative test, record
results based on the relative level of
For
a semi-quantitative
test,
record
intense
red-violet colonies
present.
results based on the relative level of
intense
coloniesa present.
You
mayred-violet
wish to choose
semi-
quantitative test if you take different
You maydepending
wish to choose
semi- level
actions
on thearelative
quantitative
if you take
present, andtest
if recording
andifferent
actual
actions
on the relative level
numberdepending
is not required.
present, and if recording an actual
number is not required.
Listeria level should be recorded as
categories that are meaningful to your
Listeria level
should
beyour
recorded
as plant
sampling
location
and
individual
categories
that are
meaningful
to your
standards (e.g.,
low,
medium, high,
or
sampling
and your individual plant
acceptablelocation
and unacceptable).
standards (e.g., low, medium, high, or
acceptable and unacceptable).
15
15
For a qualitative test, record results of
the plate as detected or not detected
For
a qualitative
test, record
resultsofof
based
on the presence
or absence
the
platered-violet
as detected
or not detected
intense
colonies.
based on the presence or absence of
You
mayred-violet
wish to choose
a qualitative
intense
colonies.
test if a yes/no answer is sufficient and
You
may
wish
to
choose
a qualitative
appropriate for your reporting.
test if a yes/no answer is sufficient and
appropriate for your reporting.
Not detected
Detected
Not detected
Detected
For customer service, call 1300-735-292
To fax an order, use 1800- 067- 639
Visit us online: www.thermofisher.com.au
©2010 Thermo Fisher Scientific Inc. All rights reserved. A.B.N. 52 058 390 917