Current status of MSIRI germplasm collection
Transcription
Current status of MSIRI germplasm collection
ICT Applications in Research & Development in Agriculture World Telecommunication and Information Society Day 2015 Telecommunications and ICTs: Drivers of Innovation Mauritius Sugarcane Industry Research Institute, MCIA 18 May 2015 • GEOGRAPHICAL INFORMATION SYSTEM • SOFTWARE DEVELOPMENT • BIOINFORMATICS GEOGRAPHICAL INFORMATION SYSTEM Examples of output for decision-support: Maps & added-value data Geographic Information System (GIS) is an information system for storage and management for describing places on earth SUGARCANE FACTORY AREAS SUPPLYING ALTEO LEGEND Planters category Miller/corporate - 14 557 ha Other Total - 8 294 ha - 22 851 ha Other features Factory area boundary Main roads Rivers Motorway Isohyets (mm/yr Prepared by Irrigation & Land Resources Department, MSIRI July, 2014 Source : i. GISCANE (MSIRI, 2012) ii. SIFB data, 2012 iii. Topographic Map of Mauritius 1:25,000 Series Y881 (D.O.S 329); Ed 6-OSD 1989 Iv FORMOSAT image (CNES, 2008) v. Mauritius Meteorological Services vi. Factory area boundary (SIFB, 1995) MECHANISATION SUITABILITY MAP OF MAURITIUS (PROVISIONAL) Fully mechanisable Intensive derocking Derocking,redesign drainage & special harvest Redesign drainage & special harvest Mechanical loading only Marginal land Water body Factory area limit Ex-tea land 2010 Land use map of Mauritius Preparation of Land Use map using satellite image (2009-2011) N Approx. Scale 1: 350 000 Source: CHUNG TZE CHEONG, M., RAMASAMY, S. and ADELAIDE, S. (2011). The 2010 Land use map of Mauritius. Reduit: Mauritius Sugar Industry Research Institute. 22 p.: 7 tbls, 1 fig., and 1 map (scale 1:100 000). (MSIRI Occasional Paper No. 41). © MSIRI, 2011. Sugar cane varieties recommendation for small planters PETIT RAFFRAY LA SALETTE MON LOISIR FACTORY AREA SOILS GOODLANDS ESPERANCE TREBUCHET RECOMMENDED VARIETIES H M3035/66, M695/69, M52/78, M1246/84, M1394/86, M1400/86, M2256/88, M703/89, M1861/89, M2593/92, R570, R573, R575, R579 L1 M1176/77, M261/78, , M1246/84, M1400/86, , M2024/88, M2256/88, M703/89, M1672/90, M2593/92, M1989/99 R570, R573, R575 L2 M695/69, M1176/77, M52/78, M261/78, M1246/84, M1400/86, M2024/88, M2256/88, M703/89, M1672/90, M2593/92, M1989/99, R570, R575, R573, R579 P1/P2 P3 ROCHE TERRE POUDRE D’OR HAMLET SCHOENFELD PITON RIV. DU REMPART AMITIE PLAINES DES ROCHES BELLE VUE MAUREL M1176/77, M261/78, , M1246/84, M1400/86, , M2024/88, M2256/88, M672/90, M2593/92, R570, R573, R575 D M1176/77, R570, R573 S M3035/66, M1176/77, M1400/86, R570, R573, R575 T3/T4 M1176/77, R570, R573 No recommendation SUGGESTED VARIETIES Source : Recommendation sheet No. 183, June 2012 – MSIRI, MCIA AMAURY La Nicolière MARE D’AUSTRALIA Future outlook: Convergence of ICT solutions for farmers Digital data capture Drone The overall objective is to improve the a) field productivity for adapted cultural practices to reduce the production costs and increase yield; and b) the capacity in the industrial/regional resource planning. Data viewing or situation assessment for almost Real-time solutions GPS survey Overlays of related spatial data Through Web portal and Server agricultural land Isohyet Soil Family Satellite imagery Digital Elevation Model SOFTWARE Irrigation management APPLICATION OF ICT FOR IRRIGATION • Development of an irrigation scheduling software • For management of an irrigated block • Integrated irrigation management tool • Irrigation Management Information System (IMIS) for sugar cane FEATURES OF SOFTWARE IMIS GIS-based and integrating biophysical factors related to irrigation in a farm provision of standard format for data capture, storage & manipulation enable unbiased decision-making in irrigation management. IMIS Development of software • Data collected from sugar estates in Mauritius & overseas partners • GIS map layers prepared • Irrigation optimization models developed and tested • Software designed • Interfaces, screens & reports developed • Software modules developed and integrated • Modules tested individually, system tested as a whole 1. Main menu Projet ACP/EU 2.4 2. Required Inputs The IMIS software - GIS map - Irrigation Systems Administrator Input of parameters Log in /Password - Fields details - Soil data - Water flow IMIS Users Log in /Password Daily data input - Rainfall - Evaporation - Previous irrigation 3. Outputs – Irrigation priority, Soil Moisture graphic displays Testing in the field: easy to use Table of irrigation recommendations & priority 30B 43B 30A 43A 17A 4C 17B 17D 17C 4D 4B 4A 44A 44B 29A 52B 53B 29C 29B 53A 52A 51B 6B 6A 6C 6D 51A 22D 22A 22C 22B Graphical display: Rainfall, Irrigation, Soil Moisture Status Soil moisture status of irrigated block BIOINFORMATICS Analysis of complex biological data Sugarcane Variety Identification Morphological characteristics – subject to interpretation Fullstand internode bud ligule DNA Fingerprinting of Sugarcane – less subjective DNA from sugarcane analyzed in genetic analyzer Use Bioinformatics software for data analysis Peaks/profile pattern unique to the variety obtained Varieties Profile Allows identification of unknown Variety Varieties ‘Unknown’ M 1334/84 R 570 R 575 Profile Allows paternity testing – identification of the parents from which a variety was derived Construction of sugarcane chromosome map Generate nearly 1 terabyte of DNA sequence data Process data using bioinformatics software and high performance workstations (servers) Identify molecular markers and construct genetic map (chromosome) of sugarcane (SBI-01) (SBI-02) (SBI-03) (SBI-04) (SBI-05) (SBI-06) (SBI-07) (SBI-08) (SBI-09) 110-130 chromosomes in sugarcane Approximately 30-50 markers placed along each chromosome (SBI-10) Marker Assisted Breeding Associate presence of marker to presence of desirable trait Identify markers linked to 1) resistance to disease 2) Early ripening and high sucrose 16 14 12 10 8 6 4 Minor gene Major gene conferring resistance Indirect selection of resistant plants at an earlier stage of breeding cycle 2 0 0 3 6 9 121417202224262932353841444750535659626568717477798285889194 Sugarcane chromosome 28 Accelerate sugarcane breeding cycle Reduce resources and cost Increase efficiency of breeding Morphological identification of pests 6 species of the sugarcane armyworms pest present in Mauritius M. pseudoloreyi M. phaea M. insulicola Mythimna sp. larvae M. loreyi Confusions in identification due to similarity at species level DNA barcoding Specimen Extract DNA DNA barcoding enables precise identification of pest species Measures to mitigate impact of the pest can be taken rapidly COI gene PCR DNA sequencing Sequence analysis L loreyi Australia M. loreyi Lepidoptera sp l.stenographa M curvula (Kenya) M. pseudoloreyi M conigera L ursula L adjuta L lapidaria M. phaea M phaea (Kenya) Lepidoptera sp. L insueta M. insulicola L striata (Madagascar) L striata (Madagascar) M. tincta M ferrago M. pyrausta Lepidoptera sp. HM914193 M Ferrago HM874912 Development of specific pathogens PER HAW BRA REU CHN1 IND Highly specific primer design using bioinformatics software – enables precise and sentive diagnostic Conclusions