docChris Lach Reem Daher-Nahhas John Schoenbeck Lawrence
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Chris Lach Reem Daher-Nahhas John Schoenbeck Lawrence
Chris Lach Reem Daher-Nahhas John Schoenbeck Lawrence Technological University BME Senior Capstone Project Dr. Mansoor Nasir, Fall 2012 • Major Advantage: Significant reduction in patient morbidity • Tissue Engineering Triad Scaffold should Resemble ECM (composition, structure, surface chemistry, mechanical properties, etc.) Objective: Three-dimensional biomimetic braiding design. Characterization Methods: •Biomimetic Structure •Mechanical Properties •Cellular Biocompatibility Scaffold Model Fiber Identified Model Benefits & Limitations Double-Triple Helix + Maximum Tensile Strength ‐ Reduced Toe Region Twisted Triple Helix + Maximum Toe Region ‐ Hard to maintain fiber Alignment Pleated Triple Helix + Tunnels for Cell Migration ‐ Minimal Tensile Strength Research Study Biomimetic Structure Mechanical Properties Biocompatibility Parameter of Interest Testing Method Braid Angle Kinovea Video Analysis Braiding Tension Force Transducer/Custom Guide Fiber patterns ESEM Imaging Porosity Porosimeter Pore Size Distribution Porosimeter Ultimate Tensile Strength Tensile Test using MTS Elastic Modulus Tensile Test using MTS Maximum Strain Tensile Test using MTS Toe Region Tensile Test using MTS Cell Proliferation Immunostaining, alamar blue assay, and confocal microscopy Cell Alignment Confocal Microscopy and ESEM Month Milestone September Brainstorming & Research Analysis October Fiber Braiding Clamp Fabrication November Establish Experimental Protocols December Tension Measurement Guide Fabrication January Biomechanical Structure Analysis February Mechanical Testing & Cell Culturing March Cell Seeding & Biocompatibility Tests April Data Analysis & Publication Manuscript May Final Report & Presentations • Electrospun poly(ε-caprolactone) fibers provided by University of Michigan • Cells and testing equipment provided by Beaumont, General Motors, and Lawrence Tech • Custom braiding tension apparatus & Cell assay kits. ~$1000 • Variability in braids produced by hand. • Braiding machines available but expensive • Optimal pattern not yet characterized • Designated Braiding Specialist: Chris Lach • Custom Built Force Measurement Guide • Find an optimized biomimetic braiding pattern • An established set of protocols for evaluation of scaffold structure, mechanical properties, and biocompatibility • • • • Custom built fiber braiding machine Future studies in bioreactor processes More rational ACL scaffold designs Applications in other fibrous tissues Team Members • Reem Daher-Nahhas • Experimental protocols • Data collection & analysis • Reporting tables & appendices • Christopher Lach • Scaffold braiding & Sampling • Cell culturing & staining • Reporting figures & references • John Schoenbeck • Scheduling & documentation • Scaffold & device design • Reporting writer Advisors & Collaborators • Dr. Yawen Li (Faculty) • Dr. Hsiao-Ping Moore • Beaumont Orthopedics Laboratory • Dr. Kevin Baker • Tristan Maerz (Technical) • University of Michigan • Dr. Joseph Corey • Sam Tuck • General Motors [1] Lanza, Langer & Vacanti (ed.) 2007. Academic Press, San Diego: 2007. ISBN: 9123206157 [2] Cooper, J. 2002. Drexel University, Thesis. http://hdl.handle.net/1860/31 [3] Vaquette et. Al. 2009. J. Biomedical Materials Research. 2010; 94(4): 1270-1282. [4] Cooper et. Al. 2004. Biomaterials. 2005 May; 26(13):1523-32
