Ultracentrifuges

Ultracentrifuges
Sales and Support in the US and CA by:
NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447 | 763.553.1270 | WWW.NUAIRE.COM/HITACHI
These general-purpose ultracentrifuges have the highest
performance in their class* — 100,000 rpm (CP100WX)
— making them ideal for the wide range of separation
tasks used in cell biology, biochemistry and ano-material
fields. For reliability and efficiency to match their speed
and quiet operation, they incorporate automatic rotor-life
management (RLM), a large color LCD display and
positive-feedback (click-type) touch pad entry. Other
standard features include RCF (x g) computation and display
functions and real-time control (RTC) for direct
entry of start/stop times and other parameters. A log options
list permits connection with a PC (for simulations and logging
of rotor and centrifuge operation histories), printer
connection, user security functions and more.
*Versus other similar ultracentrifuges in this class, as of October 2012.
1
Advanced technology
A New Era of Rotor Life Management
Rotor life is automatically managed
Operation records of each rotor are stored in the RLM (Rotor Life
Management) memory and the RLM element embedded in the rotor,
eliminating need for manual rotor logbook entries. (Naturally, non-RLM
rotors still require manual logging.)
Rotor life is automatically extended
Automatic rotor life management system records rotor type, serial number,
total number of runs and accumulated run time of the rotor. By precise
automatic tracking of the rpm profile and operation time to the nearest 0.1
hours and 0.1 times means, the rotor life is extended as much as 10 times
longer than as conventional calculation.
The highest acceleration and RCF
100,000 rpm and RCF to 803,000 x g*
The highly efficient and compact drive unit can reach maximum acceleration
of 100,000 rpm in 5 minutes (CP-100WX). The motor spins in a vacuum, with
a unique wire-suspension design.
*Versus other similar ultracentrifuges in this class, as of October 2012.
Powerful imbalance protection
Samples need to be balanced within 5 mm by visual check only. Noncontact imbalance sensor always monitors vibration of the rotor and
drive shaft. In case of abnormal vibration, the sensor activates and stops
operation immediately.
Drive unit warranted for 10 years
Safety and environmental impact reduction
0° is maintained by electronic cooling
An electronic cooling system, based on a thermo-module maintains
samples at 0°, even at maximum acceleration (ambient temperatures
to 30°).
Energy savings
Power consumption is reduced by 10% (in-house comparison) through
the use of an IGBT inverter.
Exceptionally low noise – 53 dB or less
Based on EU requirements for mechanical and
electrical safety and electromagnetic compatibility
Brake control system for reverting motor electric
power to the power source side
Heating during deceleration is thus curbed.
Environment friendly product
About 80% of the centrifuge proper is composed of recyclable materials.
Ultracentrifuges
2
Easy operation
Microcomputer control functions
The liquid crystal screen simultaneously displays set values and the actual
operating conditions.
Color liquid crystal display and
touch-sensitive panel
Keypad input is enhanced by gentle “click” feedback to confirm correct
entry. Operation errors or faults are immediately indicated by means of an
alarm display.
Low table height easier rotor handling
The operating height of the work
top was lowered to 33½ in (85
cm), making the large-sized rotors
easier to load and unload.
Real-time control (RTC) simplifies
timer settings
Operators input desired start times directly and can input both start time and
operating profile in advance for unattended operation.
User name is shown on screen,
linked to user ID code
The user name can be shown through ID
code input. This function allows interactive
verification of users.
Powerful customization functions
These functions can be selected through the interactive screen display.
• Setting of date
• Print-out function (optional)
• User registration
• Setting of the zonal rotation speed
(2,000 – 3,000 rpm)
• Setting of time
• User time reservations
• Screen contrast adjustment
• Identification number of the
centrifuge unit
Biosafety option
In order to prevent from exhausting bio-hazardous sample
to a room, a HEPA filter can be assembled in a vacuum line
at option.
3
Operating parameters are automatically
computed and displayed, based on selected RCF
RCF max. and RCF avg. at the selected
rotational speed are automatically calculated
and displayed for a given rotor, reducing
bothersome calculations. The rpm and
operating profile are calculated simply by
setting the desired RCF.
User lockout
When sharing a centrifuge under joint
utilization or in RI facilities, the optional
lockout system limits utilization to registered
personnel only.
Data communication
Optional LAN board is available. You can link CP-WX series to your PC through ethernet or internet. Optional software “himac LogManager Ver. 3.1
(network edition)” can be installed in your PC and operating history and data of CP-WX series can be easily managed by your PC.
himac LogManager Ver. 3.1 for Windows® (Network Edition)
himac LogManager Ver. 3.1 for Windows® (Network Edition) is useful and convenient
software to manage real time operating log of himac CP-WX series, CS150NX, C150FNX,
CS22N and CR-GIII series. Maximum 16 units of the abovementioned centrifuges can
be registered to the software. As data communication between the centrifuge and PC is
done via LAN or Ethernet, so there is no distance limitation to establish a network. You
can easily establish the network configuration and relocation with commercially available
LAN devices. (Optional LAN board is required for the centrifuges.) It means you can
manage the operating log at the different location from the installation site of
the centrifuges.
- Optional log management software
This software is real-time log management software; recording period is selectable from 10
seconds to 5 minutes interval. Of course, the software supports U.S. FDA 21 CFR Part 11.
The following functions are available:
• Digital Signature
• Audit Trail
• Encrypted Data Files etc.
Compatible OS:
Windows® XP, Windows Vista®, Windows® 7 Professional
Windows®, Windows® XP, Windows Vista® and Windows® 7 are registered trademarks of Microsoft
Corporation in the United States and other countries.
himac ASSIST
- Optional centrifugation
simulation software
himac ASSIST is simulation and calculation software of centrifugal condition and can
be installed in your Windows®-based PC. You can simulate whether the centrifugal
condition is appropriate before the centrifugation, also can simulate optimal centrifugal
condition of a sample, whose centrifugal condition is unknown, based on your
ultracentrifuge and rotor.
himac ASSIST has following functions:
• Calculations of K factor and pelleting time
• Calculation of the allowable rpm with high-density liquid
• Rate zonal simulation
• Isopycnic simulation
• Solvent concentration conversion
• Mutual conversion of molecular parameters
• Rotor database
Ultracentrifuges
4
Rotors
Self-locking rotor system
himac original “self-locking rotor system” is the most simple system to set the rotor onto the drive shaft.
You just simply place the rotor on the drive shaft and that’s all. The rotor is locked by centrifugal force
automatically. You do not need to fix the rotor by screw or push the button to lock or unlock the rotor like other
models available in the market.
S-cap series
himac original S-cap series offer simple and easy operation of the tube cap. This unique system
requires just three components and three steps to assemble the cap to the tube.
• No consumables, such as O-ring
• Easy cleaning and no maintenance
Three assembling steps:
1. Fill sample into the tube up to 80% volume and insert AL Stem into the tube by the tool.
2. Assemble Ti Ring on the tube and tighten AL Stem and Ti Ring by the tool.
3. Fill up the sample into the tube by an injector and close Setscrew.
P32ZT: Zonal centrifugation system
P32ZT
Zonal rotor
Part No. 9023160M
In zonal centrifugation, a density gradient is developed within the zonal rotor
running at low speed. Next, the samples to be separated are loaded through
the center of the zonal rotor and are separated at a preset high speed. After
separation, the gradient is unloaded and collected through the center by injecting
high-density fluid from the outside wall of the zonal rotor while the rotor is running.
The desired fractions are moved into the fraction collector via a spectrophotometer
using a flow cell which monitors and records the optical density of the fractions.
Separation Procedure
P32CT: Continuous flow centrifugation system
The Model P32CT Continuous Flow Rotor, designed for use with Hitachi preparative ultracentrifuges, enables you to perform highly
efficient continuous flow separation and purification of large volumes under high centrifugal force.
Product made to special specification
P32CT
Continuous flow rotor
Part No. 9022660M
940 ML core ass'y.
Part No. 348333A
Configuration of P32CT
5
For separating samples containing much sediment or performing separation after increasing the density gradient, the 940 ml
core ass'y increases the rotor capacity to 940 ml. The flow volume performance is about 35% less than that of standard cores.
System flow diagram
Rotor list
Type
Model
(automatic)
Max.
speed
Max. RCF
(rpm)
(x g)
Nominal
capacity
K-factor
Main
purpose
(ml x
number of
samples)
Main purpose
P100AT2*
9023113M
100,000
803,000
6.5 x 8
18
*
P90AT
9023053M
90,000
700,000
12 x 8
25
P80AT*
9023093M
80,000
615,000
12 x 8
27
P70AT2
9022753M
70,000
452,000
12 x 12
36
P70AT
9022623M
70,000
505,000
40 x 8
44
Separation of Norovirus in food products
Rapid separation of intermediate-to-large volume sample
P65A*
9022313M
65,000
370,000
12 x 10
48
Separation of cell organelles, Plasmid DNA and RNA
High-speed separation of intermediate volume sample
P50AT4
9024111M
50,000
316,000
6.5 x 44
P50AT2*
9022633M
50,000
*
*
Fixed
angle
P100AT2
Part No.
(outsidetubecavity)
303,000
40 x 12
32
(outsidetubecavity)
Separation of microscopic particles and lipoprotein
Separation of cell organelles, Plasmid DNA and RNA
Rapid separation of intermediate volume sample
Separation of lipoprotein
70
Separation of Norovirus in food products
Rapid separation of intermediate-to-large volume sample
P50A3
9023140M
50,000
252,000
1.5 x 24
33
Rapid pelletting micro volume samples
P45AT*
9022643M
45,000
235,000
94 x 6
130
Rapid separation of large volume samples
P42AT
9022651M
42,000
223,000
0.23 x 72
12
Rapid separation of lipoprotein for examination
(for micro volume polyspecimen)
P32AT*
9023150M
32,000
111,000
12 x 32
186
Simultaneous processing of polyspecimen
P27A
9023121M
27,000
106,000
160 x 6
352
Separation of large volume samples
(pretreatment for manufacturing vaccines)
Separation of large volume samples
P19A
9022221M
19,000
55,100
230 x 6
754
P90NT*
9023073M
90,000
646,000
5x8
10
P65NT*
9024153M
65,000
402,000
12 x 10
23
P65NT2*
9023103M
65,000
431,000
5 x 18
15
Processing of many tubes of plasmid DNA (5 ml x 18 tubes)
*
P100VT
9023063M
100,000
700,000
5x8
6
Rapid separation of plasmid DNA
P65VT2*
9023023M
65,000
416,000
5 x 16
10
P65VT3*
9023033M
65,000
402,000
12 x 10
13
Separation of plasmid DNA etc.
by the sedimentation equilibrium centrifugation
P50VT2*
9023083M
50,000
243,000
40 x 8
36
Density gradient centrifugation of large volume samples
P65ST
9022391M
65,000
419,000
5x3
48
Density gradient centrifugation of protein, DNA and RNA, etc.
P56ST
9022551M
56,000
409,000
4x6
54
P55ST2
9022771M
55,000
366,000
5x6
50
Density gradient centrifugation of micro volume samples
Sucrose density gradient centrifugation of protein, DNA
P40ST
9022371M
40,000
284,000
13 x 6
139
P28S
9022881M
28,000
141,000
40 x 6
252
Zonal
P32ZT
P32ZT
9023160M
32,000
102,000
1,690 ml
363
Large-scale purification of protein and virus particles by
density gradient centrifugation
Continuous
flow
P32CT
P32CT
9022660M
32,000
102,000
430 ml
42
Continuous concentration of large virus particles
Neo
angle
P90NT
Vertical
P100VT
Swinging
bucket
P28S
Notes: 1. Automatic life management rotors, CP-α, CP-MX and CP-WX series-compatible only
(automatic RLM system).
2. * They can be purchased from "Rotor Guide" on the website.
(http://centrifuges.hitachi-koki.com/rotor/)
3. All the rotors above are also compatible with the CP-α, CP-MX and CP-WX, series.
4. Rotors with model names that include the letter T are made of titanium alloy.
5. When model names do not include the letter T, rotors are made of aluminum alloy.
6. Buckets for swinging bucket rotors are made of titanium alloy.
Rapid separation of Plasmid DNA and RNA
Density gradient centrifugation of intermediate volume samples
Sucrose density gradient centrifugation of virus particles
7. When using the zonal rotor P32ZT, the RPZ-S zonal rotor seal attachment ass'y.,
Part No. 90130600, is required (order separately). Please refer to p. 5.
8. When using a seal tube, please place a separate order for tube sealer and tube rack.
9. ● indicates a made-to-order rotor.
10. Capacity in the above table shows nominal capacity of tubes. Actual capacity in actual use
may be smaller than the nominal capacity depending on rotor structure, tube shape, etc.
11. As for P32CT, refer to p. 5.
12. The P28S rotor can also be used for 16 ml x 6 pcs., with optional 16 Ti bucket ass'y.
(Part No. 347607A, order separately).
Unique rotors
P50A3
The P50A3 fixed angle rotor can separate
samples contains in twenty-four 1.5 ml
microtubes at a time. It is suitable for speedy
pelleting of minimal volume samples in nanoorder. Fluid annulus prevents the sample leakage
even if the sample overflows from a tube in the
rotor during the rotor is spinning. Applicable
microtube is "himac 1.5 ml microtube" (S308892A,
300pcs/box) only.
P32AT
The P32AT fixed angle rotor can separate samples
contained in thirty-two 12 ml tubes at the same RCF at
a time. It is suitable for separation of many samples in
nano-order at 100,000 x g or higher RCF. Fluid annulus
prevents the sample leakage even if the sample
overflows from a tube in the rotor during the rotor is
spinning. Tube cavity angle 35° in two layers provide the
same RCF to all tubes (32 tubes) for efficient pelleting.
Ultracentrifuges
6
Specifications
Model
Max. speed (rpm)
Max. RCF(xg)
CP100WX
CP90WX
CP80WX
100,000
90,000
80,000
700,000 (P90AT rotor)
615,000 (P80AT rotor)
803,000 (P100AT2 rotor)
Speed control accuracy (rpm)
Set speed (rpm)
±10 (1,000 to maximum speed)
1,000 to maximum speed in increments of 100
Displaying and setting RCF
Applicable (RCF max. or RCF average)
ω 2 T run
Applicable
Drive unit warranty
10 years
Rotor temperature control accuracy (°C)
±0.5 (set temperature is from 0 to +40)
Cooling method
Thermo-module cooling (CFC/HCFC/HFC-free)
Operation panel
Color liquid crystal display (256 color) and keyboard
Touch-sensitive panel (run screen only)
Rotor life management
Data communication /
Log Management system
Vacuum system
RLM rotor: automatic management
Rotor with optical adaptor: management by registration system
LAN board (option), himac Log Manager Ver. 3.1* (optional software)
Compatible OS: Windows® XP, Windows Vista®, Windows® 7
Oil-rotary vacuum pump with moisture removal function and oil diffusion pump
HEPA filter
Option
Operational noise (dB(A))
53
Heat radiation into the room
Dimensions (w x h x d)
1 kW or less
31 x 27 x 39 in (790 x 690 x 1000 mm);
depth with safety cover: 35 in (890 mm); height to table: 33½ in (850 mm)
8¾ ft2 (35½ x 35½ in) [0.81 m2 (900 x 900 mm)]
Floor area
Weight
882 lbs (400 kg)
Power requirements
Single phase, 50/60 Hz,
AC 200/208/220 V ±10%, 20 A max. (normally 8A)
AC 230/240 V ±10%, 16 A max. (normally 7A)
CE marking qualified
EN 61010-2-020, EN61326-1, EN61000-3-2*, EN 61000-3-3*
Standard
*These standards are applicable to the product when AC230/240V is selected.
Note: Windows®, Windows® XP, Windows Vista® and Windows® 7 are registered trademark of Microsoft Corporation in the United States and other countries.
1-8-12 Shiba-Kouen, Minato
Tokyo 105-0011 JAPAN
TEL : +81-3-5733-0206
FAX : +81-3-5733-0207
URL : http://centrifuges.hitachi-koki.com
All specifications are subject to change without advanced notice.
Actual color may vary from the color of the photos on this catalogue, due to printing conditions.
Due to safety reasons, installation environments, operating environments and conditions may be restricted.
• Unless specially mentioned, products and/or operation panel of the photos are standard specification.
• For further information, please contact your nearest Hitachi Koki’s representative.
•
•
HLI-7B
2013.07
•
Sales and Support in the US and CA by:
NuAire, Inc. | 2100 Fernbrook Lane | Plymouth, MN 55447
tf: 800.328.3352 | ph: 763.553.1270 | fx: 763.553.0459
Learn more at nuaire.com/hitachi