Kem-En-Tec Nordic A/S

NEWSLETTER 1. 2016
Kem-En-Tec Nordic
COMPETENT BIOSCIENCE SINCE 1987
Credit: Stephen Dixon
Liquid automation
- Easy & Fast
page 6
Glucan labelling
- Dramatically increased FLR
and MS sensitivity page 6
Mirus Transfection reagents
- Efficient & Non-Toxic!
page 7
WB Imager
- German quality way
below market price page 3
Nordic
NEW TMB substrate
- Now OD >4.0 possible! page 4
DNA removal from RNA
- One-step page 5
Contact info:
Finland
+358 9594822
Sweden/
Norway +46 18 149000
Denmark +45 3927 1777
info@kem-en-tec.com
All prices are valid until
30. June 2016.
Local currency exclusive
VAT and Ex Works.
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Copenhagen LabMed
Lokom
October 7-9, 2014
“We find the unit extremely
easy to operate, convenient
to work with and delivering
a bright picture in a very
short time”
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Multi-Rotators – Two sizes
What’s on display?
Capillary
electrophoresis
Delivered with
platform
that fit– all
Fragment
Analyzer
the
diameter tubes
up to
15 for
mmyour
Ø–
perfect
tool
Platforms available
mm holes.
DNA andwith
RNA 30
analysis
DESCRIPTION
CAT # NEW
Learn tips and tricks
from our product
specialists
“The Celvin® S provides
Prof Ivan Roitt, Middlesex University of London
CHOICE OF MOVEMENT:
Easy switch
between Vert
ical/
Horizontal
rocking
LIST PRICE DKK
BS-010117.AAG-04
Molecular biology
Revolutionary NEW
Visit
our ®booth
WB imager
- Celvin
S
high quality images as well
Attend
a demonstration
as efficiently
designed hardand software”
and have
a chance to
Prof. Dr. Christina Strube, PhD University of
win
prices
Veterinary
Medicine, Hannover, Germany
See last page for
information regarding
address and tickets
CAMPAIGN PRICE
MULTI BIO RS-24
5.310,-
3.985,- DKK
5.110,- SEK
5.110,- NOK
535,- €
MULTI See
RS-60
the newestBS-010118-AAI-04
clever
gadgets for visualization
of DNA
7.615,-
5.715,- DKK
7.330,- SEK
7.330,- NOK
765,- €
Laboratory Centrifuge
The world’s
most efficient
polymerases
Liquid handling
Experience the Kapa
Biosystems solutions
first hand
DESCRIPTION
CAT # ElectrophoresisBS-010208-AAA
LMC-3000
and Fractionation
• MW protein fractionator GelFree8100 – An ideal pre MS analysis
tool
• RunBlue SDS-PAGE – Precast gels with
superior resolution and so strong
that they show no tears!
• InstantBlue – The 15 min Coomassie –
Quick and Easy gel staining
LIST PRICE DKK
12.750,-
F forc
ON/OF
braking
CAMPAIGN PRICE
9.565,- DKK
12.265,- SEK
12.265,- NOK
1.275,- €
DESCRIPTION
MICROSPIN 12
2
Multilabel reader with
monochromator- great
CAT # introduction discounts
BS-010213-AAI
79.995,- DKK
102.550,- SEK Liquid handling
104.050,- NOK CyBi®-FeliX
10.670,-€
Compact pipetting platform
Easy, compact and sensitive
WB imaging
Small yet sensitive
Cold
R
oom
or
Rotor f ns
operation
colum
ELISA
(4°C)
• Crocodile ELISA mini workstation
NEWSPIN
– Run your ELISA for you
• Plate washers 8 and 96 channel
For
2,0/1,5
ml tubes and 0,2 ml
– Also
goodml
fortubes,
Plate 0,5
coating
up to &
12.400
xg
•tubes
ELISAand
Readers
automation
–
LED based solutions
• ELISA Reagents – Ready to use
LIST PRICE DKK
CAMPAIGN PRICE
and safe
8.880,-
6.660,- DKK
8.540,- SEK 8.540,- NOK
With its compact size, Celvin S finds room in
every lab.
Lab
instruments
See and try the
hot new laboratory instruments
from BioSan
888,- €
Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777
for fully automated single- to
multichannel liquid handling
Simply place your WB or microplate on top,
close lid and operate by touch screen !
Celvin™ S can even image microtiter plates.
High speed mini-Centrifuge
New Tristar² S
Campaign price!
ed
Upgrad ed
Low noise bench top centrifuge for operation with microtiter plates, tubes
up to 50 ml and Gel Cards for blood group serology. Choice of rotors sold
separately also with 25 % discount. Ask for quote.
CyBi®-SELMA
Semi-automatic 96- or
384-fold pipettor
The high sensitivty is obtained by keeping
the distance from sample to detector short in
combination with a high sensitivity camera.
Diagnostics-
and research
Contact
product specialist Peter Andersen for
more
kitsinfo pba@kem-en-tec.com
• ELISA
• PCR
• NGS kits
Research
Sensitive
& High
reagents for
resolution
16-bit
camera
• Glycobiology,
• Cell
biology
4,2
Mpixel
• Flow cytometry
Binning
up to 6x6
Multilabelreader
Sense: Microplate reader
with touchscreen
– it has
Touchscreen
never been
easier
display
Place your
WB or microplate here
Most of the news
will also be shown at
Lab14 in Norway, see
last page for moreLid locks
information
during
aquisition
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
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PCR
D Decontamination kit
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Copenhagen
October 7-9, 2014
A
NEW
Lokom
Heat&Run
gDNA removal kit
™
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Irreversibly heat labile enzymes for removal
”Nu har jeg prøvet InstantBlue og det er da
DNA
contaminants – at low temperature
• Conveniently remove DNA contaminations fromof
PCR
mastermixes
InstantBlue – The 15 Minute
Coomassie stain
What’s on display?
bare et fantastisk produkt ;0) Det skal vi helt
sikkert have •
indført
her ibackground:
laboratoriet – sender
Reduce
improve target detection
en bestilling afsted med det samme ;0)”
Jytte Bryde
Rigshospitalet
• NoClausen,
negative
effect on
PCR sensitivity
• Efficient for ordinary PCR and probe based qPCR mixes
Capillary
electrophoresis
Fragment F
Analyzer
REE – the
perfect tool for your
samanalysis
ple
DNA and RNA
Molecular biology
See the newest clever
gadgets for visualization
of DNA
Experience the Kapa
Biosystems solutions
first hand
Product Description
CAT # DESCRIPTION
5280A
TMB X-tnd
• MW protein fractionator GelFree8100 – An ideal pre MS analysis
tool
• RunBlue SDS-PAGE – Precast gels with
superior resolution and so strong
that they show no tears!
• InstantBlue – The 15 min Coomassie –
Quick and Easy gel staining
New Tristar² S
VOLUME
DKK
SEK
1 L Kit Contents:
2.230,- 2.860,-
NOK
2.860,-
€
305,-
• dsDNase (100/500 reactions)
• DTT (Inactivation Aid)
Decontamination Protocol
See last page for
information regarding
address and tickets
MIX
JUST
NOK See and€ try the
hot new laborainstruments
2.732,- tory
288,from BioSan
9.608,- 1.013,-
Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777
HEAT
CyBi®-FeliX
Compact pipetting platform
for fully automated single- to
multichannel Incubate
liquid handling
20 minutes, 60˚C
Quality Control: The kit is tested for absence of RNase.
10 minutes, 37˚C
5 minutes, 58˚C
Research
reagents for
IMAGINE
• ELISA Readers & automation –
DKK
SEK
LED based solutions
• 2.660,-
ELISA Reagents 2.128,– Ready to use
2.732,- and safe
9.365,- 7.492,- 9.608,-
Liquid handling
Incubate
The Heat&Run kit is suited for gDNA
20 minutes, 37˚C
removal of RNA from any source.
ow!
Storage Conditions:
at -20°C.
Try it nStore
er 20 %
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Amintra™ Ni NTA
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Sample Material:
discouThe kitpcan
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affinity resin
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contaminating
DNA
both
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qPCR mixes.
some
to SYBR based qPCR mixes.
listAlso
For Purification of HIS tagged proteins.
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f June
ELISA offered
The very best quality
th o
30The
Quality Control:
kit is tested for absence of RNase.
Crocodile ELISA mini workstation
at the very best• price!
– Run your ELISA for you
Lab
• Plate washers 8 and 96 channel
instruments
– Also
good for Plate coating
VOLUME
LIST
PRICE
CAMPAIGN PRICE
Multilabel reader with
monochromatorgreat
ANN0025-53
Amintra™
Ni-NTA Resin
25 ml
introduction discounts
ANN0100-53
Amintra™ Ni-NTA Resin
100 ml
4
and have a chance to
The described•protocol
is Transcription
intended
for removal
Reverse
in
win prices
same tube
The Heat&Run gDNA•removal
kitbackground:
from ArcticZymes
Reduce
improveis target
detection
designed for removal of contaminating gDNA from RNA
of genomic DNA from RNA preps prior to reverse
available
• No negative
on on
PCR
sensitivity
• Minimize
pipetting
stepstreatment,
prior to reverse transcription.
The kit effect
is based
the
transcription. After
completion
of DNase
and reducing hands-on time
The
decontamination
protocol
is
designed
for
removing
recombinant heat labile
dsDNasefor
(HL-dsDNase)
from
reverse transcription reagents can be added directly to
• Efficient
ordinary PCR
and probe
based
qPCR
• sample
All in 15inmin.
in both research and diagnostics. Taq polymerases are
contaminating
from
20
µlmixes
reactions,
but can be scaledthe purified RNA
ArcticZymes,
which is DNA
irreversibly
inactivated
at low
a same-tube procedure.
frequently contaminated with E. coli DNA, which might temperatures.
up or down
by
adjusting
the
volume
of
the
kit
contents.
•
one
tube
chemistry
•
one
tube
chemistry
This enables an inactivation step including
reduced sensitivity
and false positives
when small heating to moderate temperatures only (58˚C), which is
CAT # DESCRIPTION
VOLUMEcauseDKK
SEK
NOK
€
amounts
of bacterial DNA are targeted.
Other sources of gentle enough to preserve both quality and quantity of all
ISB1L-53 InstantBlue stain 1 L
1.510,- 1.940,- 1.940,- 205,- contamination might be dNTP’s, buffer components and present RNA. Reverse Transcription can be performed in the
primers/probes, as well as DNA introduced during handling.
same tube, thereby minimizingMix
pipetting
steps
and reducing
dsDNase,
primers
and master mix
2x MM
hands-on time.
Primers and probe
The PCR Decontamination Kit from ArcticZymes offers an
0.5 µl dsDNase
Mix RNA with kit contents
easy and affordable solution for removal of contaminatingKit Contents:
0.5 µl DTT
8-50 µl RNA
DNA. It utilizes a dsDNase to remove contaminating
Water
0.1x
volume Reaction Buffer
• 10X reaction Buffer
Liquid
handling
y
tl
n
1 µl HL-dsDNase
Convenie
®
DNA. The double-strand specific property allows
CyBi
-SELMA
• HL-dsDNase
remove DNA
Semi-automatic 96- or
decontamination
toELISA’s
occur with primers and probe present.
Works in all colorimetric
HRP labelled
s
n
o
ti
384-fold pipettor
a
in
contam
dsDNase
is irreversibly
inactivated
by heating to
Storage Conditions: Store at -20˚C.
NEW TMB X-tnd™ -Also,
For stable
results
– Even at OD’s
>4
from PCR
60°C in presence of DTT, ensuring that any template
added after inactivation remains safe from digestion.
mastermixes
Sample Material:
Electrophoresis
and Fractionation
DESCRIPTION
Learn tips and
tricks
ove
Rem
from our cproduct
ontaminating
specialists gDNA from
RNA
Heat & Run
Attend
a
demonstration
gDNA
Removal
Heat&Run
Protocol
TMB ELISA substrates
– NEW substrate for High OD’s
The world’s
most efficient
polymerases
CAT # “everybody really
Ready to use staining
reagent for all SDS-PAGE
liked instant blue, its
gels. Simply add to the
an amazing product”
gel, wait 15 min while
protein bands appear and
Pavel, BMB SDU
you’re done staining the
PCR
is
a
sensitive
method
for detecting presence of DNA
gel. As Easy as can be.
PCR decontamination
Product Description
Visit our booth
Diagnosticsand research
kits
Add template and run qPCR
• Glycobiology,
• Cell biology
• Flow cytometry
Distribute decontaminated master mix
Add template (to 20 µl total volume)
Run qPCR
Multilabelreader
Sense: Microplate reader
with touchscreen – it has
never been easier
Most of the news
will also be shown at
Lab14 in Norway, see
last page for more
information
RUN
Run RT of your choice
in the same tube
• ELISA
www.arcticzymes.com • contact@arcticzymes.com • ph: +47 77 648 900
• PCR
Sykehusveien 23, N-9019, Tromsø, Norway
• NGS kits
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
www.arcticzymes.com • contact@arcticzymes.com • ph: +47 77 648 900
5
Copenhagen
LabMed
CyBi -FeliX. Your Automated
October 7-9, 2014
®
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Application
Starts
Here
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Lokomotivvæ
CUSTOMER TESTIMONIAL:
• Precise 96 or 384 channel pipetting
• Single wells, rows and columns
• Easy interchangable heads
• Multilayer platforms - Very small footprint
”12 positions on
the 2 decks – and
it has same size
as an old PC!”
What’s on display?
”We are working with
different 96 heads and the
Choice head - changing the
pipetting head takes only
5 seconds!”
Capillary
electrophoresis
The world’s
most efficient
polymerases
MS
Extracted MS peak area of G0F
3.0E+5
Experience the Kapa
Biosystems
solutions
2.5E+5
first hand
transfecting prostate cancer cell
line PC-3 and
22Rv1 foraoverAttend
demonstration
expressing my gene of interests.
and have a chance to
We got around 60-75%
transfection efficiency.
Survival
win prices
was good (85%) depending on
the vector
use.”page for
Seeyoulast
1.5E+5
Key features:
• Dramatically increased FLR and MS sensitivity when compared to 2-AB
• Ideal for FLR screening using a 5 minute gradient
• Higher resolution can be obtained using a 60 min gradient
• Manual labelling and clean up method using the GlykoPrep platform available immediately
• Performance by MS
1.0E+5
5.0E+4
2A
e
oc
Pr
Co
m
pe
ain
ti t
am
or
id
X
S
IM
B
0.0E+0
Electrophoresis
and Fractionation
• MW protein fractionator Gel-
FLR and
MS Response
Comparison.
Free8100
– An
ideal pre MS analysis
Response of Competitor X, Procainamide and 2-AB labeled G0F.
IMS
IMS
100 x6
IMS
tool
• RunBlue SDS-PAGE – Precast gels with
superior resolution and so strong
that they show no tears!
• InstantBlue – The 15 min Coomassie –
Quick and Easy gel staining
ELISA
IMS
• Crocodile ELISA mini workstation
– Run your ELISA for you
• Plate washers 8 and 96 channel
– Also good for Plate coating
• ELISA Readers & automation –
LED based solutions
• ELISA Reagents – Ready to use
and safe
IMS
Lab
instruments
IMS
–H 2 0
600
650
700
750
800
850
900
950
1000
1050 1100
1150
1200
1250
1300
1350
1400
1450
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1550
1600
1650
1700 1750
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IMS
550
1850
1900
1950
2000
2050
2100
2150
IMS
500
IMS
450
IMS
400
IMS
350
IMS
300
IMS
250
IMS
200
IMS
150
IMS
100
IMS
IMS
–H 2 0
50
IMS
IMS
IMS
Multilabel reader with
monochromator- great
introduction discounts
IMS
IMS
–H 2 0
0
IMS
New Tristar² S
2200 2250
2300
2350
2400
2450
2500
m/z
2550
2600
See and try the
hot new laboratory instruments
from BioSan
2650
CID MS/MS of IMS-labeled A2F
6
information regarding
address and tickets
”What I like most about the TransIT
X2 is that, it is very easy to work with
TransIT X2. Furthermore we are more
than satisfied with the fast delivery and
excellent customer care.”
Liquid handling
Try our new TransIT-X2 solution, a
polymeric system for efficient delivery
of plasmid DNA, si-RNA and mi-RNA
A Novel Instant Glucan
Labelling Reagent for MS
2.0E+5
Learn tips and tricks
from our product
specialists
”I have used
TransIT X2 for
CUSTOMER TESTIMONIAL:
”If you forget to put eg a
reservoir on the deck – just
open Felix – fix issue - and
continue the program. Easy.”
”Felix is extremely flexible –
we are doing DNA purification
and transfers for PCR, working
with 600ul buffer and 2 µl DNA
during the same process!”
See the newest clever
gadgets for visualization
of DNA
Visit our booth
Eva Gertman,
Carlsberg Research
Laboratory
Fragment Analyzer – the
perfect tool for your
DNA and RNA analysis
Molecular biology
Are you
transfecting
cells?
Kem-En-Tec Nordic · Finland +358 9594822 · Sweden/Norway +46 18 149000 · Denmark +45 3927 1777
TransIT-X2® System Enables Superior Gene
Expression v.s. Lipofectamine® 2000 in a
Variety of Cell Types
• Efficient–Exceptional Broad
Spectrum Transfection
• Versatile–Cutting Edge Delivery of
Plasmid DNA and siRNA
• Novel Technology-Non-Liposomal,
Polymeric Delivery
•Perfect for CRISPR/Cas9
transfection
s on all Mirus
Free sample
reagents
Transfection
Diagnosticsct our local
free to conta
and
Feelresearch
n
alists who ca
kits
product speci
it
e the right k
elp you decid
•h
ELISA
lication.
• PCR
for your app
• NGS kits
CyBi®-SELMA
Semi-automatic 96- or
384-fold pipettor
Martuza Sarwar,
Lund University,
Experimental
Cancer Research
TransIT-X2® Dynamic
Delivery
System
Liquid
handling
®
‡
CyBi
-FeliX
HUVEC
A549‡
Compact
pipetting
platform ‡
Immortalized
Keratinocytes
AsPC-1
for
fully
automated
TM
‡
FreeStyle 293-F single- to
BHK-21
multichannel liquid
handling
BJ‡
MDA-MB-468‡
MDCK‡
BT-20
NHDF‡
Caco-2
AU565
C2C12Research NIH-3T3
BxPC-3
‡
‡
L929
CFPAC-1
COS-7
reagents for
‡
‡
LNCaP
CHO-K1
HeLa
• Glycobiology,
Lipofectamine®
DI-TNC1
HCT
116
• Cell biology PC-12
Most of the news
2000
RAW 264.7
DU-145• Flow cytometry
MCF-7
will
also
be
shown
at
T47D‡
Hep G2‡
MDA-MB-231
Keratinocytes‡
Lab14
see
HCC1143‡
PC-3 in Norway,
MDA-MB-453
HCC38
last page for more
Neuro-2a
HEK 293
SH-SY5Y
information
HMEC (epithelial)‡
Multilabelreader
SK-N-MC
8
28
Sense: Microplate reader
with touchscreen – it has
never been easier
5
‡ Cell types with >2-fold luciferase expression in head-to-head comparisons.
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
7
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JUST
IMAGINE
JUST
Meet us!
THE POSSIBILITIES
WITH AUTOMATED
IMAGINE
CRISPR ANALYSIS
THE POSSIBILITIES
WITH AUTOMATED
Exhibitions/­
Conferences
in 2016
DENMARK
June 14–17
Odense. Nordic Congress in
Clinical Biochemistry 2016
CRISPR ANALYSIS
September 27-29
Copenhagen
SWEDEN
‘’The Fragment Analyzer
allows us for fast, reproducible
Fragment Analyzer is the only automated instrument for the analysis
. Accelerate
your scientific discovery
of CRISPR/Cas9 gene-editing events
and
high throughput
quality
using a streamlined process for easy identification of both individual and pooled
control of NGS libraries’’
gene mutations.
™
Anna Koza, DTU Biosustain
More at AATI-US.COM
Fragment Analyzer™ is the only automated instrument for the analysis
of CRISPR/Cas9 gene-editing events. Accelerate your scientific discovery
using a streamlined process for easy identification of both individual and pooled
gene mutations.
Maj 23-27
Helsingborg. Infektionsveckan
och Mikrobiologiskt vårmöte på
Helsingborg Arena
Juni 1-2
Vårmöte KITM, Umeå Folkets Hus
FINLAND
August 24-25
14th Finnish Microbial
Pathogenesis Days.
Haartmaninkatu 3, 00290 Helsinki
Automating genomic discovery
September 1-2
26th BioCity Symposium
Mauno Conference Center, BioCity
20520 Turku
October 6-7
Laboratory Medicine Marina
Congress Center, 00160 Helsinki
Use it also for: Capillary electrophoresis of any nucleic acid
More at AATI-US.COM
Contact product specialist Klaus Tangsgaard for more info. kn@kem-en-tec.com
8
Kem-En-Tec Nordic · info@kem-en-tec.com · www.kem-en-tec-nordic.com
Automating genomic discovery