t{ou [t tluuvt 1 u [ueN fl 1{t t143J 1

d
,,
,
o
oitt
t{ou [t tluuvt 1 u [ueN fl 1{t t143J 1
:t
In
o.
vu
fl
fl]fi
!!
1.]1.1
Fll0.ltJ :L
n
r : n r :tdrJ : v1u
tril6uu:'
ti'lurJ r rr
um
u
v
A 1.1
qY
LYIU
du
o.o }ldfllJ1fl
a
u,J
d,
'
o.b il0'lULtJgllfl?'l
od !
ts. ri':-r
r v fr'o r u6'r l : v {fr l u {o rr i a :r o n a r : fi fi a r do llr rhi'r,i'i r 6'x
U! 6'r o
*. rf ir 6'r s o v o1o r tJ fr rifr r r uTn : r n r : n r :ldil : vI u t rin a u vr:^ dlu d r t r u ra u
d
d
-X
il:luaut0unn{u
d
m.o .1?9lYi o tlfl51FllJ bddc(
YVtV
d
d
4
dq q |
6 o
4
4d
,a4
d
o LfllUlJQl1.J0r1{r:tAU{t1J0 --PDA vt}rtAU1!41J?UYilnA!01v QrU?U do Q11J lAU.itlJ0vl}J
rulrr r*'ur,iruoudnarr
xo l^aa uln:
uu
b ri\rvrfiu{ufio:rvrvunlualuo'rl4'r:rfiurrfio pon fitritriurrJflfiruv siruru nr arar{uii
J
u 4d
o
tll0ulillanqqt0ut0
,
d s
<Aon
v 4 d
Z
d
,
'
en umSarqiln:rufr"lriLunr:aninfrl6uuo t'tiu drnanopure llnn-u lnit frr]tlttJn ltavunorl
r,i'
rov!lv\unoirl frr.jrunr:*irrfio 4n#tlnrr,{ siruru oo !0
a a#rrdrduronrlladto{ NucleospinR Plant DNA Extraction kil qiru'lu 0d aluv'iuii
a u.r-rLrJirrrudrsuuo1prui6 Polymerase Chain (PCR) nr:n:']aur6irrYl:tra6iSuuo tuav
er
r
u
u
I
eir pcR product fi6'otnr:r,rrrirrirrrafirSuroufiorirnrirlr{aitn:'rvr,i'loreri6
OY
I
d o
a
14 S
u
4
S
d
6)
Direct DNA sequencing
a
1
-
I
a
<
i
v
Iou"[dnr:iun:rvr.irirriurua?o{[:LULqJ].roaauduro"Lurir?ru SSU ttav LSU iurriuTrj:n^u0ufrairl
*o*
rrr
-.--
alpha r,tio RPBb
b
n:?ar{Frnr:r;u.i6i'reirdrSuro (DNA sequencing) n1::?lJ:'t}.Jduduro
sequencing assembt.y) :rlvirulEarifiar.lrir:aruo^rSuuotugrudorynarnaloutd}J:urn;il
(DNA
BLAST
Jaua
6n.le. {?9lT- b t9[OlJqiln1ilUd
bddc(
t
d
ut
o ra5u:raluoryr:raulrfio pon fitrilriutlfrfi?uuqn#ln1ri druru do e1u 16ulrfiofifi
u
o
Vtdaa
lJUl
Fl
tAU,..l'lUfl! UU
di
.
v
Y
o
nAl.l e(o l]0 fl L}.l Fl5
6'u
u,dr r.
r,
ua"tuo r u o r vt r : udu r
ido
P
DA fi liliei
B
r
rJ
v sir ur u *l
fr fi r u
a
r u niuti
ad
tl\ou'tx.llafloo t0ut0
d
en
,
riq
er)
u 4 d
,
Y
rn5arqrJn:nifr'trTlunr:an-a6t6uLo utiu drnanopure
r !'
' X
rov\rv\uFtoSv! vlzurunt:erttljo
d
,
d
lrndr:
s
lnil frtliJttJfl lLavua0n
I
o
vlnalflrv alu?u oo !o
< ariort'rduroenuaGro{ NucleospinR Pl.ant DNA Extraction kil dru']u 6., alufliuii
a rfrltJilrrudrsuuoToui6 Polymerase Chain (pcn) nr:n:rovrrirn'lrr:ao'u6uuo rtns
3
ril
pCR
product fi6'o.lnr:urrird'urr.ladrsurorfior,irnriFr:{aita:lvrileaiB Direct DNA sequencing
lnuldnr:irn:rvr,{rirnilrlarorl:h1clloadrduuoluLBL?ru SSU uav LSU irlrriulr.l:m'utt^ufiairl t rr
- o - alpha Y6o RPBb
b
fir
:? q ifi
Fr
nr
:
rir n-r 6 r5u ro ( DNA sequencing)
rE u r
nr
:r r o' rd u to
::?u
(
DNA
sequencing assembly) :rurituliu,.lfiarrirarudrduro"Lugru{oqaarnaloruldTrl:run::r BLAST
m.mflgtvtd m tdouu'utnrJ teddc(
o
X
a
X
!q,c,
Ytdaa
.iJUlO tal,Jr.JlUPlllU fl
A1{ elo
b rflrvreu{rfio:ryvualuaru0'r14r:ua^urrfio
o
do
j'!o
X
Q'ru tau.it1j011r-l
}.160 LIJFIT
'rvvvt
4'
d o
o,
rad
rm5uila1u0'r141:rau{r10 --PDA vrt}.rLau1!{1j?uv11nfilf]'ru a1u'lu
pDA
fitritriurrJfl^firuv Eiruru u arufi'u{
dd
"
tv!0u11.r1aflflfrt0ut0
'
o
en
r,i,
tOv!
Lv\
1.J
o0
:
ad
Y
'
drnanopure rJrn6r
rnSerrqln:ajfitr?Lunr:arirr6rsuro t'tiu
X
1^'l ?1 r.J 1
'
u fl ''r :?r'r
tfl
u
0
yt
vl n a
ad
!
d
fr'r
v
o
a'r u?
Init firliluJfl tLavuaoa
u oo !o
o
ad
u
6
a anr"odrduronl:l16to{ NucleospinR Plant DNA Extraction kil siluru b alul\uq
a urilr.JilrrudrSurolouiB Polymerase chain (PCR) nr:m:xaur6irei!tradtduto
Av
u
o
4
d
ff
a
o
"i
utav
ad
a{ PCR product frn'osnr:urrirpruuraotoutomolirnl:n:{a'ttn:rvutouio Direct DNA sequencing
Touhinr:irn:rvr,{rird'r:ruarosl:I!I,u:roa6uduro"Lulitxil sSU uav LSU irlrirTrl:n^u6ufiaYrs TFF
- o - alpha v6o RPBb
b n :'t o ud n n r : rB u r rir n-u o' rd u ro ( DNA sequencing) fl I T :'l u : ? tt 6 ud u uo ( DNA
sequencing assembLy) :rrrlr&ru.liurrfiauiYr:aruo'rduuotugrudoryaarna1erutd1rJ:urn:r BLAST
el rjrarua'r6'lo'udulofit6'ifiaurir'iloHa"Lu:vn-Lralnaurairrrfluurzuuqnrfrriierurnr:
(Phy[ogenetic tree)TouldTrJ:iun:r Bioedit, Se
d
- Al utav PAUP
drarurir6'16 udurofikiufiarrirrioqalu:vo'!arnalrairsttluutluqfrtfr{iirrrurnr:
(Phylogenetic tree)IeruldTr-l:urn:t Bioedit, Se
a tot'outuurur
m.d.i?oYlJ
-
A[ tlnv PAUP
toddc{
o rjrarua'rq-uGrdurofiteirfiurrirdo4n"[u:vd'r.la1na].Jra;'rrr{Jurre.ru4fitfrlii'nrurnr:
(Phylogenetic tree)Ierult1r]:urn:r Bioedit, Se - A|. ttav PAUPen.a l:vrirtpr^ou riuaruu bddd
a tdouuqunlnil teddct
6)
Y
ci'
Y
u
d
u
Aqv
u d d
o ilrararirnl0i0uroviLnrvtulfllt0Uatu:ua!arnal.rra:1.itllutLilu01.ttt{'l?qlurfl'l:
UU
(Phylogenetic tree)Inatd1rj:rrnll Bioedit, Se - Al ttau PAUP
b Aqlr{nnt:rJqr:-frlrulu:ltt!1J:rU{rutlanr:ilfllGlrunaoet:vUv!?ar d t6ou siruru
en.d.:?9rvlr
o
o
o
QlJl.l
Jaa
sr.b {?911i
b tglouilquluu
o rn!urorur:rdarrdo
teddc(
pDA d1:^it6iurln^fi'ruvv:n#rlorr,i
u:Tqn{"[utrnrirvir:tv'rrvra''al
rqlarirriordo (tissue culture fl.ask) siruru eio r,'Jararififiturn l.ed ql'l:'l.iLclustmn:
te rflryrasu{T'tuyran{"tuo'ty1:rflu.ird,
pon firL::nou}ut?od1uYLtfl1vtf,'u{t'ilariudoreio
d
A1UlU clo Vifi'|fifl
rmiu:roln:e{fi"LrTlun1:rv,{1ur#u.irdo:r rriu
siruru too to / X,lsnti
r.jrunr:sirrdovrn#rJnrr.i
lttu
en
d
d
n
r : rfi
I
!
-
:
? 1r
ddu
:
? :r fr'x o a
a.tvl0 t?\"ij:1J: ttau!:v0?!n:1u0
q
4
r
r
r
mi
u
l
6'r o ai
xtri
,r'',r*o rJrndu
vt"
vr
frrltlurlm u{lrfiu uavfi
u o r n 6": o d r l
Sutrio r n r-l rl n l n r llu
t
u d
u
X
a riufrnnrilrirurravcioryaroruflo:ruvuaiuriavarar,ir'uq
Tnr n xlud'r r.r{n u,,r t :ui utn v | : v q r u 6BcIu d sir ur u o o fi r a fi'ud
di
frnrorn6'rodrrfrutriornr-lr
qq
Ja
m.c, {?9lYl s,
tq0Un:nfl1nil bddc(
o rniurorv'.ltro''rord, PDA dtili?iurrjfrflruvvrnd'r-lnrri rJ::qa.i"Lurrprr{ry:-lrurrvrdul
i
"&
uclarirfio16o (tissue cutture flask) druru xo rdararir,r^firurn bd Frr:''r.lrqlurlirn:
YYv.YY
lcr
siru'ru
xo
rvrrv16ur:'rvrvraa{'[uorur:tfiu{tfio PDA fir::oodlut':nr{rrairrmrvuduruqlaeirflo16o
9U
v'larari
d
en
aAq*t
,
X
X
Y
rirunr:*irrflouna-r.Jprrv{
siruru
9l
bo
t
u <
%rl
t
,
d
t
d
j
'
rtu
druvra r.lrnEr frrJiluln rtrrtu
un5uror.Jn:nifi"LrIlunr:rl^nvrdu{rilo:r
j
rravvr
X\)a:1i
e
r i
&
X u,
d uuvlnnlv!fl'ruLtnvt0Har0{r%0:'rilvralrnaualu?\uq ilvlrJQrn0r?0u'r.iljuL}Jarn!r
n r n x"tu r-r u {n ln t :q:^ tta v | : v a ? ! 6B riu ti sir ur u o o a r o vilu {
Ja
m.d tl?9lYl d tnOUA{U1nil bddct
t
t
v
X
a ri
o lny*nnrrrirurravrio4aro{rfro:rrvrattriavfi'ravVuq frnuarnrri0dr{frutfiornrJr
n r n r r"Lu q-r u {n nnr t : qB * a v | : v o r r 6B r]u ri r u r u o o a r a v[u ii
b r,1uyr?u{or.rn rraua'nrniurronar:l:vnor.lnt:tfiuu rfiokirj:vn0!nl:nJ^uuvriqfio
Guide Book tor:rvrvta Lcilfi to siru'tu ord fl'tuv\ilid
T
sf
1
9
Jdu
sn.c(
o
{?flYl
G{
tflOUnUUlUU tedd6(
rrayd'flrrtBu].Jr0na1:l:un0!nrrLn^uu rfiotdrJ:vnolnr:tfluurau-lfio
Guide Book ros:rilura rcilrfi b qiru'ru oa aravt'uii
1,11.1vl?u?io:.la
q
b a?lzunnr:lfrrio^lrutu?lrru!:rrJ{rur.Jnn'r:ilXl-orrunaoo:uuv[1al d tdou
o
QUIJ
*.
a.
sirurt,t
oo
r.Jfl l-m
tru
o^u''1 n rufitd'Yllolull
1
u
rruyrJfrlTrru
frYr{'x6'ortrin:vvirnr:lrr 1 o'utflunr:t-nrionnr,tlru :vtrluu viorir#ltotvt't{:1tnrr
ilU
d. ulnvr;flEjfrurorfr"jr6'xvr*od"turnr:rirfintourotfrird'rrrfroFrx'u.rtAuu'ruo'utfrprqrnnr:n:vrirrlo.lhi
'---''O
------!-'!
vggaVyuadra
: u a 1 {,.1 : 1t Q't.i
9t
0.1
u. ri ro::r
fij
r.i'l
rfi ulr
:
1.1 ?.t
nj
fi lJ 0
! n I }J n ? 1 il t1J u a :.1
uri ro:
:l
ufl
ull nl :To u ti
ur.i
ruour
nr
:
tfl un r :
vt
or
{:-u'tx
0(,N
tl
I .tadY,
^
(uxallilruql:r
ttuxou)
u
v
v
Y A-
14?14U1lQ',llrUrvl,\1aE
!