Ampicillin Sodium Salt Cell Culture Tested Product Code: TC021

Ampicillin Sodium Salt
Cell Culture Tested
Product Code: TC021
Product Description:
Quality Control:
Molecular Weight: 371.39
Molecular Formula: C16H18N3NaO4S
CAS Number: 69-52-3
Synonyms: D(-) α-Aminobenzylpenicillin.Na
Appearance
White crystalline powder, hygroscopic
Ampicillin belongs to the penicillin group of β-lactam
antibiotics. It differs from penicillin by the presence of an
amino group. It inhibits bacterial cell-wall synthesis
(peptidoglycan
cross-linking)
by
inactivating
transpeptidases on the inner surface of bacterial cell
membrane. This broad spectrum antibiotic is effective
against Gram-positive and Gram-negative bacteria.
pH
8.00-10.00
In cell culture, ampicillin is used as an antibacterial agent
and as a selective agent for transformed bacteria. Many
common vectors carry genes encoding resistance to
ampicillin and are identified by the ability of the host
bacteria to grow in the presence of this antibiotic.
Resistance is mediated by cleavage of β -lactam ring of
ampicillin by β -lactamase.
Directions:
In cell culture applications ampicillin sodium salt is
generally used at a concentration of 100mg/L for
antibacterial use.
Preparation instructions:
Ampicillin sodium salt is soluble in water (50mg/ml).
Stock solution cannot be autoclaved. It should be
sterilized by filtering through a sterile membrane filter
with a porosity of 0.22 micron or less.
Solubility
100mg soluble in 1mL of water
Water (K.F.)
≤ 2%
Specific rotation [alpha 20/D]
+258° to +287° (c = 0.25% in 0.4% potassium hydrogen
pthalate)
Melting point
~215°C (dec.)
Assay
91.00-100.5%
Antibiotic Sensitivity Test
Complies
Cell Culture Test
Passes
Storage and Shelf Life:
Stability of ampicillin solution is dependent on
temperature and pH. Ampicillin in solution is not very
stable at pH above 7. The optimal pH of the stock solution
should be lower than or at pH 7. In addition, the identity
of the buffer can affect the solution stability.
Store ampicillin powder at 2-8ºC away from bright light.
Stock solution is stable for 3 days at 37°C and for
approximately 2-3 weeks at 2-8°C.
For long term storage, it is recommended to aliquot the
stock solution in sterile containers and store at -20°C.
Shelf life is 36 months.
Use before expiry date given on the product label.
Please refer disclaimer overleaf
Revision No.: 1 / 2013
Disclaimer:
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and other
related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best of
our knowledge true and accurate. HiMedia™ Laboratories Pvt. Ltd reserves the right to make changes to specifications and information related to
the products at any time. Products are not intended for human or animal diagnostic or therapeutic use but for laboratory, research or further
manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed
or implied, and no liability is accepted for infringement of any patents.
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