How To Use A Microscope Properly! Check out the Tutorial on
Transcription
How To Use A Microscope Properly! Check out the Tutorial on
How To Use A Microscope Properly How To Use A Microscope Properly! Tricks Of The Trade That Were Designed To Make Your Life Easier! http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm Check out the Tutorial on LOOKING VS SEEING! Return to Mr. Lazaroff's Anatomy & Physiology, Forensics, or Biology First of all, since the microscope costs between $600 and $800, lets try to figure out a few ways to AVOID my having to bill YOU for the cost of replacing one! Part of the cost is due to the incredible advances made in light microscope structure over time, which doesn't even take electron microscopes into account! Click To Enlarge! Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top The Parts of a Typical Microscope Click on the parts for more info! Check out Histology World's Audio Microscope for definitions and pronunciations of the parts above. Be sure to also check out the Table of Contents for Audio Histology Slides, Histology Games, and more! Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top How to figure out the Magnification NOTE: The colors of the lenses vary from the old scopes to the new scopes in our school. If you are from a different school, please write in the colors and the magnifications in the middle portion of the table below. Older 'Scopes Your School's 'Scopes Write in the COLORS, and the MAGNIFICATIONS below! http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm How To Use A Microscope Properly Eyepiece Low Power 10 x Green = GO! High Power 10 x 100 x 10 x 43 x 430 x Yellow = Caution 10 x 97 x Never Red = Use This or 100 x STOP! Lens! Eyepiece Low Power Color = _______ Green = GO! Yellow = Caution Oil Lens Objective Magnification Lens Medium Power Color = _______ 970 x High Power or 1000 x Color = _______ Oil immersion lenses are typically used with bacterial cultures, which have been sterilized on the slide, and there is NO COVER SLIP. Use of this lens with a cover slip may DAMAGE THE LENS! Oil Lens 10 x Objective Lens Magnification 4x 40 x ALWAYS start out with this lens, focus and center before moving on! 10 x ______ x ______ x Focus (Fine focus knob only!) and center before moving on! 10 x ______ x ______ x Focus (Fine focus knob only!) and center before moving on! STOP! Make your diagrams now! 10 x ______ x Eyepiece Low Power Start out A special oil is used with this type of lens to refract the light. It is NOT meant for slides with a cover slip! DO NOT USE ALWAYS Medium Power 10 x Focus (Fine Yellow = Caution High Power 10 x Focus (Fine before mov Use Caution Oil Lens ______ x Color = _______ 10 x 10 x A special oil i DO NOT USE NOTE: Once again, some of the colors may be different on your Microscope. Be sure to note the correct Colors and Magnifications in the middle portion of the table above. To get a sense of the importance of size in microscopy, see the following on Cell Size & Scale: http://learn.genetics.utah.edu/content/begin/cells/scale/ (Just drag the bar right to increase, and left to decrease, the magnification. Note, also, the scale that appears in the upper left hand corner!) GENERAL PROCEDURES: Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top 1. Make sure all backpacks are out of the aisles before you get a microscope! Always carry the microscope with one hand on the Arm and one hand on the Base. Carry it close to your body. 2. Remove the cover, plug the microscope in, and place the excess cord on the table! If you let the excess cord dangle over the edge, your knee could get caught on it, and the next sound you hear will be a very expensive crash. I will bill you later! 3. Always start and end with Low Power! The Green means GO! -- “Go ahead and put the slide on the stage.” “Go ahead and use the Coarse focus knob.” “Go ahead and remove the slide from the stage.” “Go ahead and put the microscope away.” NOTE: Some of the colors may be different on your Microscope. Write the correct color next to the sentence above. 4. Place the slide on the microscope stage, with the specimen directly over the center of the glass circle on the stage (directly over the light). Then you have a 9 out of 10 chance of finding the specimen as soon as you look through the eyepiece! NOTE: If you wear glasses, take them off; if you see only your eyelashes, move closer. Be sure to close, or cover your other eye!! NOTE: If you see a dark line that goes part way accross the field of view, try turning the eyepiece. That dark line is a pointer that will be very valuable when you want to point out something to your lab partner, or your teacher! http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm It is NOT m How To Use A Microscope Properly 5. If, and ONLY if, you are on LOW POWER, lower the objective lens to the lowest point, then focus using first the coarse knob, then the fine focus knob. The specimen will be in focus when the LOW POWER objective is close to the lowest point, so start there and focus by slowly raising the lens. If you can’t get it at all into focus using the coarse knob, then switch to the fine focus knob. 6. Adjust the Diaphragm as you look through the Eyepiece, and you will see that MORE detail is visible when you allow in LESS light! Too much light will give the specimen a washed-out appearance. TRY IT OUT!! 7. Once you have found the specimen on Low Power (100x), unless specifically asked to draw it on low power, center the specimen in your field of view, then, without changing the focus knobs, switch it to High Power. If you don’t center the specimen you will lose it when you switch to High Power (Yellow). [See Above] NOTE: Some of the colors may be different on your Microscope. Write the correct color next to the sentence above. 8. Once you have it on High Power remember that you only use the fine focus knob! The Yellow means CAUTION! -“Caution, use only the fine focus knob.” “Caution, do not remove the slide when it is on High Power.” -- The High Power Objective (430x) is very close to the slide. Use of the coarse focus knob will scratch the lens, and crack the slide. More expensive sounds . . . 9. NEVER USE THE OIL LENS. Red Means STOP!! -- “Stop! Don’t use that lens!” -- It is an oil immersion lens. Without the oil to lubricate the lens, you will destroy it! More expensive sounds . . . Also, the oil is needed to help gather enough light to actually see through the lens! NOTE: Some of the colors may be different on your Microscope. Write the correct color next to the sentence above. Tips On Making Good Drawings: Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top 1. Don’t even think of starting your drawing unless you have a PENCIL! Drawings in PEN are UNACCEPTABLE! This is for two reasons: (a) You can erase pencil! (b) You can shade in areas more easily in pencil. 2. Each Drawing must be 1/2 page in size, and must include clear, proper labels! In the upper left hand corner of each circle include the specimen name as written on the slide label. In the upper right hand corner, include the magnification (100x or 430x). 3. Labels should start on the outside of the circle. The circle indicates the field of view as seen through the eyepiece. All arrows should end with the point touching the object to be labeled! 4. Animal cells should always include at least the following five labels: Cell membrane, Nuclear membrane, Nucleus, Chromatin, Cytoplasm. 5. Plant Cells should always include at least the following seven labels: Cell membrane, Cell wall, Nuclear membrane, Nucleus, Chromatin, Cytoplasm, Chloroplast (this last does not exist in certain plant cells). 6. Remember: This class is about Connections! I don’t want you to Look at the cells; I want you to SEE them! In order to do that, you MUST: Apply your knowledge of cell structure to your drawings! An unlabeled drawing is nothing more than scratches on a piece of paper! http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm How To Use A Microscope Properly Check out the Tutorial on LOOKING VS SEEING! How To Make A Wet Mount: Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top 1. Gather a thin slice/piece of whatever your specimen is. If your specimen is too thick, then the coverslip will wobble on top of the sample like a see-saw: 2. Place ONE drop of water directly over the specimen. If you put too much water over the specimen, then the coverslip will float on top of the water, making it harder to draw the specimens as they float past the field of view! 3. Place the coverslip at a 45 degree angle (approximately), with one edge touching the water drop, and let go. How To Stain a Slide: Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top 1. Place one drop of Methylene Blue stain on one edge of the coverslip, and the flat edge of a piece of paper towel on the other edge of the coverslip. The paper towel will draw the water out from under the coverslip, and the cohesion of the water (due to those perennial favorites - Hydrogen Bonds) will draw the stain under the coverslip. 2. As soon as the stain has covered the area containing the specimen you are finished. The stain does not need to be under the entire coverslip. If the stain does not cover the area needed, get a new piece of paper towel and add more stain until it does. 3. Be sure to wipe off the excess stain with a paper towel, so you don’t end up staining the objective lenses. 4. You are now ready to place the slide on the microscope stage. Be sure to follow all the instructions on the previous pages as to how to use the microscope. 5. When you have completed your drawings, be sure to wash and dry both the slide and the coverslip and return them to the correct places! 6. All slides must be put away in the proper trays! Students will not leave until all materials have been put way properly. You are a team! Final Note: NOTE: These procedures will remain the same, regardless of the type of stain, or the addition of a hypertonic/hypotonic solution to your specimen. REMEMBER: Be careful with the equipment, and be sure to leave the lab in the same condition it was in when you arrived. HAVE FUN! Check out the Tutorial on LOOKING VS SEEING! Microscope Parts Magnification General Procedures Drawing Tips Making a Wet Mount Staining a Slide Final Note Top Return to Mr. Lazaroff's Anatomy & Physiology, Forensics, Biology, or the SCIENCE DEPARTMENT page http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope.htm Microscope Magnification Table Microscope Magnification Table Your School's 'Scopes Older 'Scopes Eyepiece Low Power 10 x Green = GO! High Power 100 x 10 x 43 x 430 x Yellow = Caution 10 x 97 x Never Red = Use This or 100 x STOP! Lens! Eyepiece Low Power Color = _______ Green = GO! Yellow = Caution Oil Lens Objective Magnification Lens 10 x Medium Power Color = _______ 970 x High Power or 1000 x Color = _______ Oil immersion lenses are typically used with bacterial cultures, which have been sterilized on the slide, and there is NO COVER SLIP. Use of this lens with a cover slip may DAMAGE THE LENS! Newer 'Scopes Write in the COLORS, and the MAGNIFICATIONS below! Oil Lens Color = _______ 10 x Objective Lens 4x Magnification 40 x ALWAYS start out with this lens, focus and center before moving on! 10 x ______ x ______ x Focus (Fine focus knob only!) and center before moving on! 10 x ______ x ______ x Focus (Fine focus knob only!) and center before moving on! STOP! Make your diagrams now! 10 x ______ x ______ x A special oil is used with this type of lens to refract the light. It is NOT meant for slides with a cover slip! DO NOT USE Low Power Start out Medium Power Yellow = Caution High Power Use Caution Oil Lens Eyepiece Objective Lens Magnification 10 x 4x 40 x ALWAYS start out with this lens, focus and center before moving on! 10 x 10 x 100 x Focus (Fine focus knob only!) and center before moving on! 10 x 40 x 400 x Focus (Fine focus knob only!) and center before moving on! STOP! Make your diagrams now! 10 x 100 x 1000 x A special oil is used with this type of lens to refract the light. DO NOT USE It is NOT meant for slides with a cover slip! http://shs.westport.k12.ct.us/mjvl/biology/microscope/microscope_table.htm Looking vs. Seeing: A Tutorial on Getting the MOST out of your Microscope Viewing! Looking vs. Seeing A 15 Minute Tutorial on Getting the MOST out of your Microscope Viewing! Check out Histology World's Table of Contents for Audio Histology Slides, Histology Games, and more! Return to: How to Use a Microscope To get a sense of the importance of size in microscopy, see the following on Cell Size & Scale: http://learn.genetics.utah.edu/content/begin/cells/scale/ (Just drag the bar right to increase, and left to decrease, the magnification. Note, also, the scale that appears in the upper left hand corner!) Define Each Term: How are they DIFFERENT? Looking: Light passes through the lens of the eye, and the vitreous humour, hits the retina, which stimulates rods (B/W) & cones (color) to send images are sent along the optic nerve . . . Seeing: The brain actually processes the information, based upon prior knowledge, and assigns meaning to the images received. Parts of the Brain Involved: When we LOOK, light hitting the Retina at the back of the eye sends a message along the Optic Nerve also known as the 2nd Cranial Nerve (N II) passes through the Optic Chiasm, by which the Sensory Data crosses over from Right to Left, and vice versa, then along the Optic Tract to the Lateral Geniculate Nucleus of the Thalamus (Diencephalon) to the Primary Visual Cortex of the Occipital Lobe. The Sensory Data is then PROCESSED, allowing us to SEE! Why are we Talking about this: I have noticed many of my students over the years giving me drawings made while looking under the microscope that in no way resemble the actual tissues observed under the microscope. These drwings almost always either have no labels, or the labels are so ill-conceived as to be of little value. The usual response from students when I point these things out is "That's what I saw." Given our definition of seeing above, it is clear that far from seeing, these students were only looking. Had the students actually been seeing the specimen, not only would their drawing have been more accurate, but all of the visible eukaryotic cellular structures would have been properly labeled (The structures that follow depend upon the specimen being observed: cell wall and/or cell membrane, cytoplasm, nuclear membrane, nucleus, nucleolus, flagellum, cilia). NOTE: Even though students may understand that they are looking at cells, it is VERY common to get drawings that fail to have cell membrane boundaries, and which focus only on the nuclei, which are usually mislabeled as cells, and usually just drawn as a bunch of dots. After completing this tutorial, you should be able to focus your prior knowledge about cells and tissues upon what you are looking at, and you will most likely then be seeing it for the first time. Your drawings will not only be more accurate representations of the specimens, but they will also contain more accurate labels. Am unlabeled drawing is little more than random scribbles on a piece of paper. Labels indicate that you actually were seeing the cells. Before we continue w/ looking and seeing, we need to emphasize the issue with an analogy:: Listening vs. Hearing. There is another page at the end of the following tutorial that compares microscope photos (photomicrographs) with the drawings that you should be able to make from them. Listening vs. Hearing Why are We Talking About Listening & Hearing Now? One of the best ways to appreciate the difference between Looking and Seeing is to step back and compare Listening vs. Hearing. The best way to truly understand the difference is to listen to a piece of music twice: the first time without preparation, and the second time using the new knowledge you are about to receive, which will help your brain to interpret the music differently! Define Each Term: How are they DIFFERENT? http://shs.westport.k12.ct.us/mjvl/biology/microscope/looksee.htm Looking vs. Seeing: A Tutorial on Getting the MOST out of your Microscope Viewing! Listening: Compressed waves of air (sound waves) hit the typanic membrane (ear drum), the vibration is amplified through the auditory ossicles (the bones of the inner ear), and the vibrations cause compressions in the fluid within the cochlea, causing the hair cells to and images are sent along the vestibulocochlear nerve . . . Good Vibrations . . . Better Vibrations . . . Hearing: The brain actually processes the information, based upon prior knowledge, and assigns meaning to the sounds received. Parts of the Brain Involved: When we LISTEN, compression waves in the Cochlear Fluid stimulates Hair Cells sends a message along the The Cochlear Branch joins the Vestibulocochlear Nerve, also known as the the Eigth Cranial Nerve (N VIII) travels through the Vestibular and Cochlear Nuclei of the Pons and the Medulla Oblongata, and then travel through the Medial Geniculate Nucleus of the Thalamus to the Auditory Cortex of the Temporal Lobe. The Sensory Data is then PROCESSED, allowing us to HEAR! What Better Example for Our Analogy than MUSIC! A Crash Course in Music: The basic elements of music: Melody = the succession of notes played one after another (the horizontal aspect of the music) Harmony = the sound when two or more notes are played simultaneously (the vertical aspect of the music) Rhythm = the number of beat per measure (4/4, 3/4, 6/8, etc.), as well as the pattern of beats used in the measure (4 equal beats, 1 beat then 2 faster beats then 2 beats like the first) Tempo = the speed at which the music moves (i.e., the time a certain beat in the music is held), from adagio (very slow) to allegro (very fast) Timbre = the specific sound of the instrument, from the wooden sound of a clarinet to the brassy sound of a trombone Dynamic Level = the volume of the music, from pianissimo ( pp = very soft) to fortissimmo (ff = very loud): ppp . . . pp . . . p . . . mp . . . mf . . . f . . . ff . . . fff Now Let's Use This To Listen to a Piece of Music! Listen to this Movement from a Schubert Piano Sonata: Try to identify any patterns in the music as you listen. Try to identify the number of beats in the music (Most Rock & Roll is built on 4 beats -- it is in 4/4 time, hence the "boom, thwack, boom, thwack" alternation of bass drum on the 1st and third beats, and snare/symbols on the 2nd and fourth beats, "one, two, three, four." Most Jazz, on the other hand, emphasizes the 2nd and fourth beats in4/4 time, "one, two, three, four."); this leads to frustration when performing in front of audiences unaccustomed to Jazz, as such an audience will clap on one and three, while the band will emphasize two and four! Listen for any changes in dynamic level, tempo, or timbre. Click HERE to hear a MIDI file of the 3rd Movement, a Minuet, from Schubert's Piano Sonata # 18 in G major, "Fantasia," D. 894, Opus 78 Franz Peter Schubert 1797 - 1828 DON'T CLICK ON THE LINK BELOW UNTIL AFTER YOU LISTEN ALL THE WAY THROUGH THE MINUET! Now that you have listened to the minuet, click on the link below to learn about the FORM of a Minuet. After learning the FORM you need to listen to the music ONE MORE TIME! The difference this time is that, having armed yourself with knowledge about musical form, you will actually HEAR the music for the first time!!! Move on to The Form of a Minuet . . . or Return to How to Use a Microscope http://shs.westport.k12.ct.us/mjvl/biology/microscope/looksee.htm The Form of a Minuet The Form of a Minuet Return to: How to Use a Microscope or Looking vs. Seeing Of the Things I Asked You to Listen For: The minuet is a form of Dance music, albeit of a different century (the 18th), and it is full of many repeats. The basic form is called an ABA form, but there are many repeats within that basic patten The minuet is somewhat akin to a form of music that followed called the waltz, in that it is written in 3/4 time, with three quarter notes (the beats) per measure (although the rhythm may vary, the number of beats in each measure remains 3 throughout. Although the dynamic level (or volume) may have changed between sections, and the timbre changed with each change of instruments, the tempo (or pace) of the music remained constant throughout. The Actual Form of a Minuet From Schubert's Time: [:A:] [:Avar A1:] [:B:] [:B var B1:] [A] [Avar A1]] The A & B sections: are based upon different melodies, harmonies, and instruments. [:A:] = [A A] Avar The "A" Section will be repeated = a variaton basesd upon a theme or themes from the "A" Section A1 = a repeat of the "A" Section, but with some modifications, usually at the end [:Avar A1:] = [Avar A1 Avar A1] [:A:] [:Avar A1:] = [A A Avar A1 Avar A1] [:B:] = [B B] Bvar The "A" Section will be repeated = a variaton basesd upon a theme or themes from the "B" Section B1 = a repeat of the "B" Section, but with some modifications, usually at the end [:Bvar B1:] = [Bar B1 Bvar B1] [:B:] [:Bvar B1:] = [B B Bvar B1 Bvar B1] Written: [:A:] [:Avar A1:] [:B:] [:B var B1:] [A] [Avar A1]] Heard: A A Avar A1 Avar A1 B B B var B1 Bvar B 1 A Avar A1 This time when you listen, look at the pattern and try to figure out not only the basic ABA form, but also each of the 15 landmarks above. http://shs.westport.k12.ct.us/mjvl/biology/microscope/minuet.htm The Form of a Minuet Now listen to the minuet ONE MORE TIME! Click HERE to hear a MIDI file of the 3rd Movement, a Minuet, from Schubert's Piano Sonata # 18 in G major, "Fantasia," D. 894, Opus 78 Franz Peter Schubert 1797 - 1828 Did You Hear It? Did you figure out where each of the 15 landmarks were? If You Didn't Hear it . . . . . . then you need to listen to the music one more time . . . If You Want to Test Your New Knowledge of Musical Form On Another Minuet -This One's Not So Easy -- Find Out If You Can Hear The Structure of This One . . . Click HERE to hear a MIDI file of the 3rd Movement, a Minuet, from Mozart's Symphony # 41 in C Major, K. 551, "The Jupiter Symphony," 1788 Wolfgang Amadeus Mozart 1756 - 1791 If that Minuet was too hard, try this easier one . . . Click HERE to hear a MIDI file of the 3rd Movement, a Minuet, from Mozart's Symphony # 39 in Eb Major, K. 543, 1788 Wolfgang Amadeus Mozart 1756 - 1791 For the Curious: For the curious, can you listen for (and hopefully hear) the structure of a simpler minuet, this one is written in a style and form much more akin to those written in Johann Sebastian Bach's day (1685 - 1750) [:A:] [B Bvar] [:A:] I wrote this minuet for my wife, for Mother's Day in 1997. THE NEXT STEP: Looking vs. Seeing Part II - Using Actual Microscope Images! Return to: How to Use a Microscope or Looking vs. Seeing http://shs.westport.k12.ct.us/mjvl/biology/microscope/minuet.htm Looking vs. Seeing Part II - Using Actual Microscope Images! Looking vs. Seeing Part II Using Actual Microscope Images Return to: How to Use a Microscope or Looking vs. Seeing or The form of a Minuet Check out Histology World's Table of Contents for Audio Histology Slides, Histology Games, and more! To get a sense of the importance of size in microscopy, see the following on Cell Size & Scale: http://learn.genetics.utah.edu/content/begin/cells/scale/ (Just drag the bar right to increase, and left to decrease, the magnification. Note, also, the scale that appears in the upper left hand corner!) Resolution: In your study of cells you may very well come across images similar to those below. Some things to consider: These cells are in a level of detail that FAR EXCEEDS the magnification possible with a Compound Light Microscope. In order to get the kind of resolution illustrated below, you would need to use an Electron Microscope. As such, very few of the structures illustrated below are visible with a Compound Light Microscope. Animal Cell Plant Cell Bacterium More Information More Information More Information All Images are from an INTRODUCTION TO CELL AND VIRUS STRUCTURE created by Molecular Expressions, a wonderful website created by faculty at Florida State University Prior Knowledge: We will be using this to determine what we are LOOKING at, so that we might actually SEE it! What is actually visible with a Compound Light Microscope will act as the prior knowledge that will allow us to comprehend what we are viewing through our eyepiece. This just leaves one question: What is visible with a Compound Light Microscope? Animal Cell Plant Cell Cell Membrane Cell Wall Cytoplasm Cell Membrane Nuclear Membrane Cytoplasm Nucleus Nuclear Membrane Chromatin (Its in there, even if you can't see the detail!) Chromatin Nucleus Bacterium It's SO SMALL, all you will see is The Cell Envelope (Composed of, from the inside/out a cell membrane, a cell wall, and in some species, a capsule.) Chloroplast Drawing Tips: Before you start to practice your drawings, you should refer to the TIPS ON MAKING GOOD DRAWINGS, which can be found on my HOW TO USE A MICROSCOPE PROPERLY page. In addition to tips on labeling, you must, above all, remember to: Apply your knowledge of cell structure to your drawings! An unlabeled drawing is nothing more than scratches on a piece of paper! Drawing Cell Images From Light Microscopes: C O M I N G SOON! 1. Practice first by making pencil drawings of the following images. 2. Include the appropriate labels on your drawings. 3. Once you have completed steps 1 & 2 above, you should check out the labeled drawings below to see how those drawing should appear. How Your Drawings should Appear: O.K. Folks! You are Now Ready to Try to Draw On Your Own, Using Real Microscopes & Slides! HAVE FUN! Return to: How to Use a Microscope or Looking vs. Seeing or The form of a Minuet http://shs.westport.k12.ct.us/mjvl/biology/microscope/looksee2.htm NSTA Conference Presentations (Present & Past) NSTA Conference Presentations http://shs.westport.k12.ct.us/mjvl/lazaroff/nsta.htm Michael Lazaroff Anatomy & Physiology, Forensics, Biology Staples High School 70 North Avenue, Westport, CT 06880 To Facilitate the Sharing of Materials from my NSTA Presentations, I have placed all the relevant files here. NOTE: I have been gradually changing the format of my handouts, which hopefully better reflects what it was like attending my presentations. Should you have any comments or suggestions, please feel free to share them here. 2011 - San Francisco - March 10th to March 13th, 2011 NOTE: All of these files were posted on the NSTA site How to Find a Specimen Quickly Under a Microscope Tired of helping frustrated students find microscope specimens? Here is a time-tested method that will have them finding and drawing, accurately, within seconds! Saturday, March 12 8:30–9:00 AM Marriott San Francisco Marquis, Golden Gate Salon C3 How to Find a Specimen Quickly Under a Microscope (Includes Links to web pages I built on how to use a microscope.) Dissection: How to Make the Most of It End your year with dissection. Learn strategies to create a safe environment, engage all your students, and eliminate the need for alternative assignments. Saturday, March 12 12:30–1:30 PM Marriott San Francisco Marquis, Sierra A Dissection - How to Make the Most of It! (Includes Links to all three dissection labs: Earthworm, Crayfish, & Bullfrog.) I am hoping to see you in San Francisco in 2011! 2010 - Philadelphia NOTE: All of these files were posted on the NSTA site, under NSTA Communities Bringing History, Art, and Literature into the Biology Classroom Make your teaching interdisciplinary. Bring in history, art, literature, and your course will come alive, and you will bring in students who previously hated science! History, Art, Literature & Biology Handout Teaching Evolution: Meeting the Challenge of S0-Called Iintelligent Design Rather than skirt the issue, meet it head on! How to use the problems with intelligent design to strengthen your teaching of evolution! Evolution Handout Evolution Powerpoint (This is a work in progress . . .) Exploring Body Systems Conceptually: How to Link Every Biology Unit to Human Body Systems Time for body systems? Every unit can be reinforced by linking to the structure and function of body systems, making dissection a true culminating activity. Body Systems Handout Body Systems Powerpoint (This will be available later.) The Dead T-Shirt Contest! Featured in the NSTA Blog, "CSI Philadelpha" http://nstacommunities.org/blog/2010/03/20/csi-philadelphia/ Participants will determine the cause, mechanism, and manner of death in an activity in which the students act as both victims and forensic pathologists. Dead T-Shirt Handout Dead T-Shirt Files Dead T-Shirt Activity Dead T-Shirt Answers (NOTE: You can use these to create your own versions of these shirts. Should you come up with your own designs, please share them, and I will be happy to post them here for others to use!) 2009 - New Orleans http://shs.westport.k12.ct.us/mjvl/lazaroff/nsta.htm NSTA Conference Presentations (Present & Past) The Ideal Mate Project: Authentic assessment in the construction and interpretation of Student’s own family pedigree! Students collect family phenotypes, construct their family’s pedigree (even the adopted), determine their genotypes, and use them to predict possible kids with their ideal mate! Ideal Mate Handout Frog’s Blood vs. Human Blood: Comparing RBC as a means to understand Cellular Respiration and SA/V Students compare RBC under the microscope and discover connections to SA/V, Cellular Respiration, Endothermic & Ectothermic metabolisms, and the evolution of body systems. Frog's Blood Handout The Dead T-Shirt Contest! See in the NSTA Blog from 2010, "CSI Philadelpha" - http://nstacommunities.org/blog/2010 /03/20/csi-philadelphia/ Participants will determine the cause, mechanism, and manner of death in an activity in which the students act as both victims and forensic pathologists. Dead T-Shirt Handout Dead T-Shirt Files Dead T-Shirt Activity Dead T-Shirt Answers (NOTE: You can use these to create your own versions of these shirts. Should you come up with your own designs, please share them, and I will be happy to post them here for others to use!) 2008 - Boston The Student Construction of Schematics to Illuminate Complex Anatomical Issues. Learn how to have students construct larger-than-life schematics of the body systems, illustrating anatomical connections & their physiological implications, and greatly increase your students' understanding. Schematic Instructions (This will be available later.) The Final Project (a.k.a. The Final Mandala) The Dead T-Shirt Contest! See in the NSTA Blog from 2010, "CSI Philadelpha" - http://nstacommunities.org/blog/2010 /03/20/csi-philadelphia/ Participants will determine the cause, mechanism, and manner of death in an activity in which the students act as both victims and forensic pathologists. Dead T-Shirt Handout Dead T-Shirt Files Dead T-Shirt Activity Dead T-Shirt Answers (NOTE: You can use these to create your own versions of these shirts. Should you come up with your own designs, please share them, and I will be happy to post them here for others to use!) It was a pleasure making presentations these past few years. If you have any questions regarding any of the items above, or any other items, or if you have items you want to share, please feel free to write or call, and I will do my best to contact you as soon as possible. Sincerely, - Michael J. V. Lazaroff Author of THE COMPLETE IDIOT'S GUIDE TO ANATOMY AND PHYSIOLOGY 1996 Access Excellence Fellow - http://www.accessexcellence.org Anatomy & Physiology Teacher, Forensics Teacher, Biology Teacher Staples High School 70 North Avenue Westport, CT 06880 Voice Mail: (203) 341-1415 School E Mail: Michael_Lazaroff@westport.k12.ct.us Personal E-mail: mjvlazaroff@gmail.com Web Pages (all designed, built, and maintained by myself): http://shs.westport.k12.ct.us/mjvl/lazaroff/lazaroff.htm (My Home Page) http://shs.westport.k12.ct.us/mjvl/anatomy/a&p-home.htm (My Anatomy Page) http://shs.westport.k12.ct.us/mjvl/biology/biohome.htm (My Biology Page) http://shs.westport.k12.ct.us/forensics/ (The Forensics Page, made with Mr. Rollison) http://shs.westport.k12.ct.us/mjvl/default.htm (Science Department Page) "It is not the strongest of the species that survive, nor the most intelligent, but the one most responsive to change." - Clarence Darrow (from the Scopes Trial, "The State of Tennessee vs. John Scopes," in 1925) http://shs.westport.k12.ct.us/mjvl/lazaroff/nsta.htm