2012 Sample Requirements Pathology Laboratory Guide

Transcription

Sample Requirements
Pathology Laboratory Guide
2012
Sample Requirements Edition 1
General Information
Sample Requirements
Sample requirements are listed showing the recommended collection tube.
In some instances alternative tubes may be used, eg the full range of tubes is
available for paediatric samples. Contact the relevant department to discuss:
Clinical Biochemistry ext: 5703 / 5 (01473 703703 / 5),
Haematology ext:
5709 (01473 703709) or
Microbiology ext:
5736 (01473 703736).
The volumes indicated are to identify the correct tube only and do not imply
that more than one Monovette is required for a selection of tests. Although if
clotted blood is required in more than one department please send separate
samples. If in doubt about minimum volumes for given assays, please contact
the relevant department to discuss.
Brown top White top Pink top Orange top Yellow top Green top Clotted sample
Clotted sample – no separating gel
EDTA
Lithium heparin
Fluoride
Citrate – must be filled to line or the sample will be rejected –
do not combine short samples to make one full tube
Blue top EDTA
Purple top Sedivette ESR tube – m
ust be filled to line or the sample will
be rejected
Red top EDTA
The minimum labelling criteria is surname plus two of the following: forename
or initial, date of birth, hospital number or NHS number.
For all blood transfusion samples the minimum criteria is full name (surname
and first name), hospital number or NHS number, date of birth, date and time of
collection and the sample must be signed by the person who took the sample.
Failure to meet these labelling criteria will result in the specimen being rejected.
Samples for Maternal Serum Screen must include either the hospital number or
NHS number as one of the three patient identifiers.
Samples for leukaemia immunophenotyping / cell markers (BCR / ABL, JAK2, PNH
Screen, EMA dye test and FISH), Genetics / Chromosomes (HFGE and HFE) and
tissue typing (HLA and HLA B27) must contain four points of reference, eg full
name (surname and first name), hospital number or NHS number and date of
birth.
2
General Information Sample Requirements Edition 1
Order of Draw for S-Monovette Blood Collection System
1
White
2
Brown
3
Green
4
Purple
5
Orange
6
Red
7
Blue
8
Yellow
Serum
Serum
Gel
Coagulation
ESR
Li Heparin
EDTA
EDTA
K Blood
Transfusion
Fluoride
Point of Care Testing
POCT coordinator: Alison Czarnota, bleep 917, Ext 5229 (01473 703229)
Ipswich Hospital users:
Blood glucose meters: For replacement meters, QC logbooks and batteries
please bring complete blood glucose meter box to Clinical Biochemistry. Lost
meters may incur a small charge. QC solutions are obtained from the Pharmacy
Department.
POCT equipment training: Training is available for blood gas analysers, urine
test strip meters, pregnancy test kits, Coaguchek XS plus INR meters, DCA2000
HbA1c analysers and urinary drugs of abuse screening kits. Please call the
POCT coordinator for details. Blood glucose meter training session details are
on the intranet.
e-Learning: Online competency assessments are available for many of the
POCT devices in use. Please call the POCT coordinator for details.
External Quality Assurance schemes (EQA): We are inspected under ISO 15189
and ISO 22870 which require all POCT medical devices be enrolled on EQA
schemes. Samples are sent regularly and participation by all staff that test
patient samples is mandatory. For details please contact the POCT coordinator.
Blood gas analysers: Emergency Department blood gas analyser is available
for use by trained staff only. For training and barcodes please contact the
POCT coordinator.
Blood gas samples should be collected into electrolyte balanced heparinised
syringes. Samples MUST be free from air bubbles, capped and mixed for at
least a minute before analysis. Samples not analysed immediately should be
mixed for longer.
New equipment: Any department considering introducing new POCT
equipment must seek the advice of the POCT committee. For details please
contact the POCT coordinator.
Community users:
EQA schemes, training and advice for POCT medical devices is available. Please
call the POCT coordinator on 01473 703229 or email
alison.czarnota@ipswichhospital.nhs.uk
3
General Information
Haematology / Blood Transfusion & Clinical Biochemistry
Department of Haematology
Consultants:
Dr J A Ademokun
ext 5719 (01473 703719), bleep 160
Dr I M Chalmers
ext 5256 (01473 703256), bleep 125
Dr I N Whalley
ext 5256 (01473 703256), bleep 904
Head BMS Mr Nigel Brinkley
ext 5727 (01473 703727)
Secretary: ext 5718 (01473 703718)
fax 01473 703720
Haematology Enquiries:
ext 5709 / 10 (01473 703709 / 10)
Anticoagulant Monitoring Service Enquiries:
ext 5228 (01473 703228)
Blood Transfusion Enquiries:
ext 5726 (01473 703726)
Services:
Routine Haematology, Anticoagulant
Monitoring, Blood Transfusion and
Immunology.
Department of Clinical Biochemistry
Consultant:
Dr P Twomey
ext 5708 (01473 703708), bleep 910
Head BMS Mr Dean Braunig
ext 5704 (01473 703704)
Secretary: ext 5708 (01473 703708)
fax 01473 703259
Biochemistry Enquiries:
ext 5705 (01473 703705)
Services:
Routine Clinical Biochemistry and
Endocrinology.
Laboratory Hours
The laboratory operates a 24-hour service. Full routine and emergency services
operate between 9.00 am and 5.30 pm, Monday to Friday, with a partial service
to 1.00 am. Only emergency services operate between 1.00 am and 9.00 am and
at weekends.
Urgent Requests
Urgent requests requiring immediate analysis, all blood gases and all
crossmatch requests must be notified by telephone to the department or, after
5.30 pm, by bleep 905 for Haematology and bleep 906 for Biochemistry.
All Emergency Department (A&E) requests where there is a likelihood of
4 hour breach must be notified by telephone to the relevant department.
Semi-urgent Requests
Samples received in the department between 5.30 pm and 1.00 am will be
analysed and the results available electronically within 2 hours of receipt.
There is no need to bleep for each request.
All requests from 1.00 am to 9.00 am must be notified by bleep 905 for
Haematology and bleep 906 for Biochemistry, unless the results are not
required within 2 hours.
4
Blood samples should be sent via the air tube, station 5709 for Haematology,
station 5705 for Biochemistry. CSF must be delivered by hand.
Web Browser
The introduction of a web browser within the hospital gives access to all
pathology results, even to GP-requested work. The web browser should be
available to all satellite and other hospitals in the future.
A full audit record of all enquiries including enquirer ID is logged automatically.
Any abuse of this system will be dealt with under the Trust disciplinary policy.
For details of access to the web browser contact Mr Andrew Block, Pathology
Computer Manager on 01473 704403 or extension 6403 or alternatively, Miss
Marisa Van Anson on 01473 703721 or extension 5721.
Collection of Fasting Samples
Fasting for plasma glucose is a minimum of 8 hours.
For lipids it is a minimum of 12 hours.
Anticoagulant Monitoring Service (AMS)
The Anticoagulant Monitoring Service must receive an AMS referral for both
new and existing anticoagulant patients on discharge from hospital.
To refer inpatients use Lorenzo iCM (select patient from patient list, enter
order and then select ACR or AMSR).
To refer outpatients use an AMS referral form or its PDF equivalent and send
by post or fax to 01473 703720. New outpatients can make appointments to
see the anticoagulant nurse specialist.
Please note that underfilled INR samples cannot be tested and the AMS will
request a repeat sample.
Laboratory Phlebotomist Opening Hours:
The laboratory open hours are Monday – Friday 7.30 am – 5.45 pm.
Saturday phlebotomy by strict appointment only, 9.00 am – 11.30 am,
telephone 01473 703721 for appointments.
Phlebotomy Services to Wards
Ward Rounds: Weekdays
8.00 am – 12.00 noon
Saturdays (limited) 7.30 am – 10.30 am
Sundays (limited)
7.30 am – 10.00 am
The weekend phlebotomy service is for clinically essential requests only.
This guide is by no means comprehensive. If in doubt, please refer to the
comprehensive Laboratory Manual available on the hospital intranet at
Business Units Ô Clinical Support Services and Cancer Ô Pathology Ô
Pathology Handbook. If you are still in doubt, please telephone for advice.
5
General Information
Microbiology
Laboratory hours:
Monday to Friday: 9.00 am – 5.30 pm
Saturday: 9.00 am – 12.30 pm
General enquiries:
ext 5736 (01473 703736)
Clinical interpretation:Dr Richard Kent ext 5741 (01473 703741)
Dr S Ginwalla ext 5745 (01473 703745)
Head BMS Andrew Bendall ext 5747 (01473 703747)
The department welcomes enquiries. Please do not hesitate to contact us for
information, advice or visits.
Urgent samples
• Must be arranged by telephone both during the day and on-call
• Results will be telephoned
• Samples / Tests included:
–CSF
– Sputum / NBL
– NPA for RSV (children) tested as one batch out-of-hours only
–Urine
– Wound swabs / tissue / fluids
Note: G
ent / Vanc assays performed by Clinical Biochemistry
• Other tests after discussion with microbiology consultant.
• Medical advice is always available – contact switchboard if out-ofhours.
On-call Service (not resident)
Monday to Friday: 5.30 pm – 9.00 am
Saturday:
12.30 pm – 9.00 am
Sunday:
9.00 am – 9.00 am
Bleep 907 or contact switchboard for on-call staff if no reply. After midnight
on-call restricted to CSFs. Other samples by arrangement between patient’s
consultant and microbiology consultant only.
OCM Ward Ordering
• One sample per request please.
• Completed results are available on microbiology screen.
• Urgent interim results are phoned.
Transport
All samples must be labelled and accompanied with a completed request form
with brief relevant clinical details.
Samples, except blood cultures and non-repeatable samples eg CSF, may be
sent by air tube to Microbiology.
6
Out-of-hours blood cultures should be placed in the incubator in the blood
bank room immediately after collection. All significant blood culture results
are phoned.
Non-repeatable samples should be delivered by hand to the laboratory.
All samples which are thought to contain high risk pathogens eg HIV, Hep B / C
and M tuberculosis must be labelled ‘Danger of Infection’ together with the
request form.
7
General Information
Histology and Cytology Specimen Requirements
Routine Histology – Please refer to Pathology Handbook for
comprehensive information. (Business Units Ô Clinical Support Services and
Cancer Ô Pathology Ô Pathology Handbook)
Routine histology specimens must be sent in formalin fixative in a container
appropriate to the size of the sample.
Frozen Sections
Frozen sections should be booked before the surgery is planned, this ensures
the relevant staff are available to process and analyse the sample. The sample
should be sent unfixed and handed to a member of the Histology staff.
Muscle Biopsies
Sample collection kit available, by request, from the Histology department.
Please allow 24 hours for preparation.
Nerve Biopsies
Sample collection kit available, by request, from the Histology department.
Please allow 24 hours for preparation.
Cytology
Gynae Cytology: Use Cytyc liquid-based cytology vial.
Non-gynae Cytology: Unfixed samples in a named universal with the exception
of urine samples that must be collected in a urine cytology container available
from the laboratory.
Post Vasectomy Semen Samples: Use semen analysis pack.
Semen Samples for Fertility Assessment:
Please ensure patient receives semen analysis pack which contains:
• Patient information leaflet
• Request form
• Sample collection pot
• Patient questionnaire
8
Clinical Biochemistry Sample Requirements Edition 1
Clinical Biochemistry Department
Tel: 5703 / 5 (01473 703703 or 703705)
Profiles:
U/E (E) [Urea, Na, K and creatinine]
Blood 7.5 ml Brown Top
LFT (L) [ALP, TP, Alb, Glob, ALT and bilirubin]
Bone (B) [Ca, PO4, ALP, TP, Alb and Glob]
Lipids (LIP) [Cholesterol, Triglycerides and HDL]
TFT (T) [FT4 and TSH]
TEST
SAMPLE
Acyl Carnitine
2 blood spots (Guthrie Card)
Adrenocorticotrophic Hormone (ACTH)
Blood 2 x 3.4 ml Pink Top
Send to laboratory immediately on ice.
Alanine Transaminase (ALT)
Alcohol (Ethanol)
Postmortem / clinical samples only.
Albumin (ALB)
Aldosterone
Blood 3.4 ml Pink Top.
Separate EDTA (Pink Top) sample
required for this assay.
Usually performed with Renin assay.
Alkaline Phosphatase (ALP)
ALP Electrophoresis (ALPE)
Alpha-1 Antitrypsin (A1AT)
Alpha Foetoprotein (AFP)
Aluminium (AL)
Blood 9.0 ml. Special tube required.
Contact laboratory.
Amiodarone (AMIO)
Amino Acids (AA)
Blood 2.0 ml Orange Top
Ammonia (AMM)
Blood 2.0 ml Orange Top.
Contact laboratory BEFORE sample
collection and transfer sample to
laboratory within 30 mins on ice.
Amylase (AMY)
Androstenedione (AND)
Angiotensin Converting Enzyme (ACE)
9
Clinical Biochemistry
Anion Gap
Aspartate Transaminase (AST)
Anti-Mullerian Hormone (AMH)
Send to laboratory immediately.
Aspirin (Salicylate)
B12
Beta-2 Microglobulin (B2M)
BHCG
Bicarbonate (Serum) (CO2)
Bile Acids (BILE)
Bilirubin (Conjugated) (BIC)
Bilirubin (Total) (BIT)
Bilirubin (Unconjugated) (BIU)
Bismuth
Blood 3.4 ml Pink Top
Blood Gas
Collect 0.3 ml of arterial blood into
commercial syringe or capillary with
no air bubbles present. Blood gases
must only be collected by trained
personnel. Blood gas samples can be
sent to the laboratory via the air tube
system.
Bone Alkaline Phosphatase (ALPB)
C2H5OH
CA12-5
CA15-3
CA19-9
Cadmium
Caeruloplasmin (CP)
Calcitonin
Send to lab immediately on ice.
Calcium (CA)
Calcium (adjusted for albumin)
10
Carbamazepine (CAR) [CBZ]
(immediately prior to dose)
Carbon Dioxide Serum (CO2)
Carboxyhaemoglobin (COHB)
Carcino-embryonic Antigen (CEA)
Carotene
Protect from light.
Chloride (CL)
Cholesterol (CH)
Cholesterol (HDL)
Cholesterol (LDL)
Cholinesterase
(organo-phosphate poisoning)
Cholinesterase Phenotype
(dibucaine number etc)
Chromium
Blood 9.0 ml. Special tube required
Contact laboratory.
Chromogranin A
Contact laboratory before collection.
Chromogranin B
Contact laboratory before collection.
CK (Total)
CO2 (Serum)
Cobalt
Blood 9.0 ml. Special tube required
Contact laboratory.
Copper (CU)
Cortisol (COR)
Must state time of collection.
C Peptide
C Reactive Protein (CRP)
Creatine Kinase (CK)
Creatinine (CR)
Cryoglobulin (CRY)
Deliver to laboratory immediately.
11
Cyclosporin (CYC)
1-25 Dihydroxy Vitamin D (1-25-di-OH
cholecalciferol) [125D]
11 Deoxycortisol
Contact laboratory.
Dehydroepiandrosterone Sulphate (DHEAS)
Dibucaine
Digoxin (DIG)
collected 6 hr post dose.
Disopyramide
E2 (Oestradiol)
Ethosuximide
Fat Soluble Vitamins (FSV)
Ferritin
FK 506 (Tacrolimus) [TAC]
Flecainide
Folate
Follicle Stimulating Hormone (FSH)
State date of last menstrual period
if known.
Free Androgen Index (FAI)
Free Light Chains (FLC)
Free T3 (FT3)
Free T4 (T)
Galactosidase
Send to laboratory before 12 noon
Mon – Weds only.
Gamma GT (GGT)
Gamma Glutamyl Transferase (GGT)
Gastrin
Contact laboratory BEFORE
sample collection.
Gentamicin
Record time of collection on request form.
12
GFR
Globulin (GLOB)
Glucagon
sample collection.
Glucose (G)
Blood 2.7 ml Yellow Top
Glucose Tolerance Test (GTT)
Contact laboratory.
Glycosylated Hb (HbA1c)
Growth Hormone (GH)
Gut Hormones
sample collection.
Haematinics
Haemoglobin A1c (HbA1c)
Haptoglobin (HAP)
βHCG (Tumour Marker) [HCGB]
Hexosaminidase
Mon – Weds only.
Homocysteine (Total) –
for investigation of arteriosclerosis risk
17 Hydroxyprogesterone (OHP)
Immunoglobulin A (IgA)
Immunoglobulin G (IgG)
Immunoglobulin G
(Subclasses 1,2,3,4) [IgGSub]
Immunoglobulin M (IgM)
Immunoglobulins (IgS)
Inhibin A & B
Insulin
Blood 7.5 ml Brown Top and Blood
2.7 ml Yellow Top for glucose assay.
Insulin-like Growth Factor 1 (IGF1)
Iron
13
Lactate
Blood 2.7 ml Yellow Top
Lactate Dehydrogenase (LD)
Lamotrigine
Lead (PB)
Lipase
Lutenising Hormone (LH)
if known.
Lithium (LI)
Magnesium (MG)
Maternal Serum Screen
Blood 7.5ml Brown Top
Samples must include either the
hospital number or NHS number as one
of the three patient identifiers.
Methaemalbumin
or 3.4 ml Pink Top freshly collected
Mercury (Blood)
Contact laboratory
Methaemoglobin
Micronutrient Screen
Blood 9.0.ml Orange Top *2
and Blood 7.5 ml Brown Top *2
Protect from light.
Myeloma Screen
Neurotensin
sample collection.
Oestradiol (17 Beta) [E2]
Osmolality (OSM)
Pancreatic Polypeptide
sample collection.
Paracetamol (PAR)
Blood 7.5 ml Brown Top collected
> 4 hr post ingestion.
Parathormone (PTH)
Send to laboratory within 8 hours
together with 7.5 ml Brown Top.
Paraprotein (PP)
Phenobarbitone (PHB)
– collect any time
14
Phenytoin (PHE)
Phosphate (PO4)
Phytanic Acid
Plasma Potassium (KP)
Porphyrins
For screening for porphyria send
random urine, random faeces &
EDTA blood at the same time.
Protect from light.
Potassium (K)
Potassium (Plasma)
Pro BNP
Procollagen Extension Peptide (P1NP)
Procollagen IIIP (PCP3) [PIIINP]
Blood 9.0 ml White Top Non-gel tube
Progesterone (PRG)
if known.
Prolactin (PRL)
Prostate Specific Antigen (PSA)
Protein (P)
Protein Electrophoresis (PE)
PTH
Renin
Send to laboratory within 8 hours
together with 7.5 ml Brown Top.
required for this assay. Usually
performed with Aldosterone assay.
Salicylate
Sex Hormone Binding Globulin (SHBG)
Sirolimus (Rapamycin) [SIR]
Sodium (NA)
Tacrolimus (FK506) [TAC]
15
Testosterone (TES)
Theophylline (THE)
– collect prior to dose
Thio Purine Methyl Transferase (TPMT)
required for this assay. TPMT assay must
now be received in the laboratory on
Monday – Wednesday only.
Thyroglobulin
Thyroid Peroxidase Antibodies (TPO)
Thyroid Stimulating Hormone (TSH)
Thyroxine (Free) [FT4]
Total CO2 (Serum) [CO2]
Transferrin
Triglyceride (TG)
Fasting Only.
Triiodothyronine (Total T3) [T3]
Troponin I (TNI)
Tryptase
Contact laboratory BEFORE sample
collection.
TSH Receptor Antibody (TRAB)
Urea (UR)
Uric Acid (UA)
Valproate
Vancomycin
Record time of collection on request
form.
Vasoactive Intestinal Peptide (VIP)
sample collection.
Very Long Chain Fatty Acids (VLCFA)
Vitamin A
Protect from light.
Vitamin B1 (Thiamine)
Vitamin B6 (Pyridoxine)
16
Vitamin B Screen
2 x Blood 9.0 ml Orange Top
Vitamin D (25 Hydroxy Cholecalciferol)
Vitamin E
– protect from light
White Cell Enzymes (WCE)
2 x Blood 3.4 ml Pink Top
Mon – Weds only.
Zinc (ZN)
Urine Specimens – collection and preservation
Test
Collection
Preservative Notes
Amino acids
Random
Nil
Amylase
24 hr
Nil
Bence-Jones Protein
Random
Nil
Bile Pigments / Salts
Random
Nil
Calcium
24 hr
Hydrochloric acid
Send to laboratory
immediately.
Catecholamines
(CATD)
24 hr
Nil
Not routinely
available,
Metanephrine
assay is more
sensitive.
Please contact the
laboratory if
catecholamines
required.
Citrate
24 hr
Hydrochloric acid
Unsuitable for
preservation on
receipt.
Copper
Random or 24 hr
Nil
Cortisol
24 hr
Nil
Creatinine
24 hr
Nil
Qualitative Cystine
Random or 24 hr
Nil
Acidified collection
acceptable.
17
Quantitative Cystine
Random or 24 hr
Nil
Drugs of abuse
Random
Nil
Electrolytes (Na / K)
Random or 24 hr
Nil
Glucose
Random
Nil
Glycosaminoglycans
Random
Nil
Haemoglobin
Random
Nil
Send to laboratory
immediately.
5HIAA – quantitative
24 hr
Glacial acetic acid
Unsuitable for
preservation on
receipt. See dietary
restrictions.
Hydroxyproline
24 hr
Nil
Send to laboratory
immediately.
Magnesium
24 hr
Hydrochloric acid
Melanin
Random
Nil
Mercury
Random or 24 hr
Nil
Send to laboratory
immediately.
Metanephrine
24 hr
Glacial acetic acid
Unsuitable for
preservation on
receipt. See dietary
restrictions.
Methylmalonate
Creatinine Ratio
Random
Nil
Send to laboratory
immediately.
Microalbumin
Random
Nil
Mucopolysaccharides Random
Nil
Myoglobin
Random
Nil
N-Telopeptide Xlinks
Random
Nil
Organic acids
Random
Nil
Osmolality
Random
Nil
Oxalate
24 hr
Hydrochloric acid
PH
Random
Nil
Phosphate
24 hr
Hydrochloric acid
Porphobilinogen
Random
Nil
18
Send to laboratory
immediately.
Send to laboratory
immediately.
Unsuitable for
preservation on
receipt.
Protect from light.
Porphyrin – screen
Random
Nil
Protect from light.
Porphyrins – 24 hr
24 hr
Nil
Protect from light.
Protein and / or
electrophoresis
Random or 24 hr
Nil
Reducing substances
Random
Nil
Steroid profile
24 hr
Nil
Sulphonylurea
Random
Nil
Total protein /
Creatinine ratio
Random
Nil
Urate
24 hr
Nil
Urea
Random or 24 hr
Nil
Urobilin
Random
Nil
VMA – Paediatric
requests only (< 18
years old). Adults
change to
Metanephrine
(METD).
24 hr
Hydrochloric acid
Unsuitable for
preservation on
receipt.
VMA
Fresh random
urine less than 4
hours old on
paediatric samples
only (< 18 years
old)
Nil
Send to laboratory
immediately.
Acidified collection
NOT acceptable.
Dietary Restrictions
Indoles are present in a number of foods and can give a false positive 5HIAA.
Suggest repeat urine collection avoiding the following foods for four days
before the collection and during the collection: bananas, avocados, red plums,
aubergines, pineapples, tomatoes, walnuts, blackcurrants, redcurrants and
chocolate.
Foodstuffs rich in capsaicin causes interference with the metanephrine assay so
must be avoided for four days before collection starts, and during collection.
These include: chilli peppers, chilli powder, curry powder and capsaicin paste.
If a test is not listed above, please contact the laboratory before starting the
collection.
If a combination of tests is requested requiring different preservatives, please
contact the laboratory for advice.
19
Haematology
Haematology Department – Tel: 5709 (01473 703709)
TEST
SAMPLE
Acanthocytes
ACRA (Acetyl Choline Receptor Antibody)
Activated Protein C Resistance
See prothrombotic screen.
Adrenal Antibodies
ANA
ANCA
ANCA MPO
ANCA PR3
Antenatal Blood Group Serology
Blood 4.5 ml Blue Top
Antenatal Haemoglobinopathy Screen (FOQ)
Antenatal Infectious Disease Serology
Blood 7.5 ml Red Top
Anti B2 glycoprotein Ab
Blood 7.5 ml Red Top
Anti Cardiolipin
Anti CCP Antibodies
Anti Choline Receptor Antibodies
Anti Endomysial Antibodies
Anti FXa assay
See Heparin assay
Anti GBM
Anti GPC
Anti LKM
Anti M2
Anti Mitochondrial
Anti Neuromyelitis Optica Antibodies
Anti Neuronal antibodies
Anti Neutrophil Cytoplasmic Antibodies
Anti NMDA Receptor Antibodies
Anti Nuclear Antibodies
Anti Nuclear HEp-2 Antibodies
Anti PDH
20
Haematology Sample Requirements Edition 1
Anti Pemphigoid Antibodies
Anti Pemphigus Antibodies
Anti Phospholipid Antibodies
Blood 7.5 ml Brown Top +
2 x 3.0 ml Green Top
Anti Smooth Muscle
Anti TTG
Antibody Screen (Immunology / Haematology) Blood 7.5 ml Brown Top
Antibody Screen (Transfusion)
Blood 4.5 ml Blue Top – see blood
transfusion labelling criteria inside
front cover.
Antithrombin
APCR
APTT
Blood 3.0 ml Green Top
APTT Ratio
Autoantibodies
Auto Immune Antibodies
Basement Membrane Antibody
B Cell Sub Sets
Deliver to Haematology by 1 pm.
Basophils
See Full Blood Count.
BCR / ABL
Beta 2 glycoprotein (anti b2gp)
Blood Group
C1 Esterase
C1 Inactivator
C3C4
Cardiolipin Antibodies
CBC
CCP Antibodies
CD3 / CD4 / CD8
See T cell Subsets.
Cell Markers
Centromere Antibody
See ENA Antibody Screen.
21
Haematology
Chromosomes
Do not take after 12 noon Friday.
Clotting Screen
Coeliac Screen (TTG)
Cold Agglutinins
Blood 4.5 ml Blue Top – keep sample
warm and deliver to Blood Transfusion
immediately. See blood transfusion
labelling criteria inside front cover.
Complement C1 Inhibitor
Complement C3
Complement C4
Complement CH50
Coombs Test
or 4.5 ml Blue Top
Crithidia Luciliae Antibodies
Crossmatch
front cover.
Cystic Fibrosis Screen
DCT
or 4.5 ml Blue Top
D-Dimer
Differential Count
Differential Leukocyte Count (DLC)
DNA Antibodies
DNA Studies for Gene Analysis
DRVVT
ELA2 Mutations
EMA Dye Test for Hereditary Spherocytosis
ENA Antibody Screen
End Plate Antibodies
Eosinophils
EPO
Erythropoetin Antibody
Erythropoetin Assay
ESR
22
Exon 12
Factor II (Factor II:C)
2 x Blood 3.0 ml Green Top
Factor II (G20210A) mutation
Factor IX (Factor IX:C)
Factor V (Factor V:C)
Factor V Leiden
Factor VII (Factor VII:C)
Factor VIII (Factor VIII:C)
Factor VIII Inhibitor
Factor VIII Profile
Factor VIII Related Antigen
Factor X (Factor X:C)
Factor XI (Factor XI:C)
Factor XII (Factor XII:C)
Factor XIII Screen
FBC
Fibrinogen
Fluorescent In Situ Hybridisation (FISH)
Fragile X Chromosome
Blood 3.4 ml Pink Top or
Blood 9.0ml Orange Top
Blood 4.5 ml Orange Top +
Free Protein S
Full Blood Count
G6PD
GAD Antibodies
Gastric Parietal Cell Antibody
GBM Antibodies
GF
Glandular Fever Screen
Gliaden Antibodies
GM1 (ganglioside) Antibodies
Granulocyte Immunology
Contact Haematology
23
Haematology
Group and Screen
front cover.
Ham’s Test
See PNH screen.
Haemochromatosis Gene
Haemoglobin A2
Haemoglobin Electrophoresis
Haemoglobin F
Haemoglobin H Bodies
Haemoglobin S%
Haemolysin Screen
Blood 3.4 ml Pink Top +
Haemosiderin
Urine.
Hb
HbA2
HbF
Hct
Heparin (Anti FXa) Assay
2 x Blood 3.0 ml Green Top – for LMWH
(eg Clexane®) take 3 – 4 hours post
injection.
Heparin-induced Thrombocytopaenia
Contact Haematology
HFE Gene Status
Histone Antibodies
HIT
Contact Haematology
HLA Antibodies
HLA B27
or 10 ml Green Top
Do not take after 12 noon Friday.
HLA Class I / HLA Class II
Blood 10 ml Green Top
HLA DR2
HLA Typing
or 10 ml Green Top
3 x Blood 10 ml Green Top +
HNF genes (specify)
IgA t-Transglutaminase Abs
IgE
24
IM Screen
Immunophenotyping
Samples must be in laboratory by
12 noon Friday.
Infectious Mononucleosis Screen
Inhibitor Screen
INR
Intrinsic Factor Antibodies
Islet Cell Antibodies
JAK 2
Jo-1 Antibody
Kleihauer
LE Cells
Live Kidney Donor
4 x Blood 10 ml Green Top
Live Kidney Recipient
Liver Antibodies
LKM Antibodies
Lupus Anticoagulant
2 x Blood 3.0 ml Green Top +
Lupus Screen
Lymphocytes
M2 Antibody
MAB Antibodies
Mag Antibody
Malarial Parasites
Blood 3.4 ml Pink Top – Detail
country visited and prophylaxis.
MCH / MCHC / MCV
Methotrexate
Monocytes
Monospot
MPO Antibodies
MTX
Musk Antibodies
25
Haematology
NBT Test
Contact Haematology.
Neurology DNA tests
Neuronal Antibodies
Neutrophil Antibodies
Neutrophil Function Test
Neutrophils
NMO Antibodies
NRBC
Osmotic Fragility
Ovarian Antibodies
Paul Bunnell
PCK2 gene
P50
PDH Antibodies
Pemphigoid Antibodies
Pemphigus Antibodies
Phospholipid Antibodies
See Lupus Anticoagulant.
Plasma Viscosity (PV)
Platelet Aggregation
Platelet Antibodies
4 x Blood 3.4 ml Pink Top +
Platelet Function Tests
Platelets
PNH Screen
2 x Blood 3.4 ml Pink Top +
Blood 3.4 ml Pink Top from control.
Deliver to Haematology before 12
noon.
PR3 Antibodies
Protein C
Protein S Free
Prothrombin Gene Mutation
Prothrombin Time
26
Haematology Prothrombotic Screen or Profile
PTS
Pyruvate Kinase
RAST
RBC
RDW
Red Cell Enzymes
Reticulocytes
Rheumatoid Factor / RF
RIPA
Ristocetin Induced Platelet Aggregation
RNP / Sm Antibody
Ro La Antibodies
Salivary Duct Antibodies
Scl-70 Antibody
Sickle Screen
Sickle Solubility Test
Skin ICS Antibody
Sm Antibody
Smooth Muscle Antibodies
Solid Organ GVHD
Specific IgE
SSA (Ro) / SSA (La) Antibody
See ENA Antibody Screen
SSB (La) Antibody
Stem Cell Confirmatory Type
Striated Muscle Antibodies
T Cell Subsets
Thalassaemia Screen
Thick Film for Malaria Parasites
Thrombin Time
27
Haematology
Thrombophilia Screen
Tissue Transglutaminase Antibodies (TTG)
Tissue Typing
Total IgE
Trial SHNL
TTG
U1-snRNP Antibody
Vasculitis Screen
Voltage Gated Ca++ Channel Antibodies
Voltage Gated K+ Channel Antibodies
Von Willebrand Disease (VWD) Screen
See Factor VIII Profile
vWF Activity / vWF Antigen
See Factor VIII Profile
vWF Multimers
WBC
ZAP 70
28
Microbiology Sample Requirements Edition 1
Microbiology Department – Tel: 5736 (01473 703736)
TEST
SAMPLE
Adenovirus CFT
Adenovirus DNA
Alpha virus
Amikacin Assay
Amoebic FAT
Anti Fungal Assays
Anti Staph Ab
ASO Titre
Aspergillus pptn
Avian pptn
Bartonella Ab
Bordetella pertussis Ab
Borrelia (Lyme)
Brucella Ab
Campylobacter Ab
Candida Ag
Cat Scratch Ab (Bartonella)
Chlamydia pneumoniae Ab
CMV IgG / IgM
CMV PCR
Colistin assay
Corynebacterium diphtheriae Immunity
Co-trimoxazole Assay
Coxiella burnettii
Cryptococcus Ag
Dengue Ab
EBV DNA Viral Load
EBV Serology
29
Microbiology
Efavirenz Assay
Enterovirus PCR
Enterovirus CFT
Escherichia coli 0157 Serology
Farmers Lung pptns
Filaria Ab
Flavivirus Ab
Flucytosine
H. influenzae b immunity
Haemorrhagic Fever Viruses –
Serol / PCR / Culture
Contact Microbiology before
sample collection.
Helicobacter pylori Ab
Hep A IgM / HAV Tot
Hep B Ag Confirm
Hep B core Ab
Hep B Surface Ab (anti-HBs)
Hep B Virus DNA
Hep C Ab
Hep C PCR
Hep C Viral Load / Genome
Hep D Virus Ab / Ag
Hep E Ab / Ag
Histoplasma Ab
HIV Ab / Ag
HIV Proviral DNA
HIV Viral Load / RNA
H. Pylori ab
Please contact Microbiology.
HSV IgG
HTLV 1 & 2 Genome
HTLV 1 & 2 Serology
Human papilloma virus (HPV) PCR
Cervical swab-smear / biopsy.
30
Hydatid Ab
Immunoglobulins for pairing with CSF
Influenza A / B CFT
Itraconazole Levels
Legionella Ab
Legionella Ag (within 7 days of onset only)
Urine (plain bottle).
Leishmania Ab
Leptospirosis Ab
Lopinavir (Kaletra) Assay
Blood 9.0ml Orange Top
Lyme Ab
Measles IgG
Measles PCR
Mumps Ab
Mycoplasma IgM
N. meningitidis Ab (convalescent)
N. meningitidis DNA (acute)
Nevirapine Level
Oligoclonal Bands
Parainfluenza CFT
Parvovirus (Erythrovirus) IgG / IgM
Polyoma BK virus DNA
Polyoma JC virus DNA
Pneumococcal Ab
Procalcitonin Assay (by special arrangement)
Psittacosis Ab
Q Fever
Rabies Serology
Resistant Screening and Viral Load
Blood 2 x Blood 4.5 ml Blue Top
Respiratory Serology
Rickettsia serology
RSV CFT
31
Microbiology
Rubella IgG
Rubella IgM
Schistosomal Ab
Spotted Fever (group)
Streptomycin Assay
Strongyloides Ab
Syphilis (TPA)
TB T-spot
2 x Blood 7.5 ml Orange Top.
Contact Microbiology before
collection.
Teicoplanin Assay
Tetanus Immunity
Tobramycin Assay
Toxocara Ab
Toxoplasma Ab
Transplant screen (Renal Unit)
Treponema Pallidum Antibodies (TPA)
Trichinella Ab
Trimethoprim Assay
Tropheryma whipplei (PCR)
CSF sample. Contact laboratory.
Trypanosomal
Varicella Zoster Virus (VZW) IgG & IgM
VDRL
VZV IgG
VZV IgM
Yersinia Serology
32
Urine Specimens
Urine containers are provided. These contain boric acid as a preservative. Urine
containers should be filled to the mark. The specimen will remain useful for up
to 48 hours at room temperature.
Small volumes of urine should be collected into plain universals and should
arrive at the laboratory within 4 hours or within 24 hours if refrigerated.
Collection of Mid-stream Urine
Instructions to the patients should be as follows:
1 Carefully wash the external genitalia and dry with a clean towel.
2Unscrew the top of the sterile container, avoiding touching the inside of
the bottle or lid.
3 Start passing urine, allowing the first part to flow into the pan.
4Collect the next part of the specimen straight into the container (women
should separate the labia with the fingers of the hand which is not holding
the sterile container). The bottle should be filled.
5Screw the lid on the container firmly. Wipe and dry the outside of the
container and write your name and the date on the label.
Collection of Urine from Infants
1 Bag Urine
External genitalia should be washed and dried before collection. Specimen
should be decanted into a sterile plain universal.
2 Supra Pubic Aspirate
These should be decanted into a sterile plain universal – these are treated
as sterile fluids and should be sent to the laboratory immediately.
Indwelling Catheter Samples
Urine should be aspirated with needle and syringe from the catheter sampling
port after disinfection of the port with alcohol.
Urine for Suspected Tuberculosis
At least 3 full early morning urine samples should be collected into large
containers (do not use small boric acid pots).
33
General Information
Sample Requirements Booklet 2012
I trust you find the booklet of sample requirements for the pathology
laboratories helpful. We intend to update it each year – please destroy any
copies from previous years.
Further copies can be obtained from Pathology reception or by completing
and returning the form at the end of this booklet.
Thank you to everyone who has contributed suggestions for improvements
to this booklet. Please continue to send suggestions by completing the form
opposite.
Please return the form to:
Stephen Gee
Clinical Biochemistry Department
Post Bag C363
Ipswich Hospital
Heath Road
Ipswich
IP4 5PD
Tel: 01473 703708
Email: stephen.gee@ipswichhospital.nhs.uk
34
Feedback on Sample Requirements Booklet 2012
I make the following suggestions for improving the booklet:
Comments:
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Name:
additional copies sent to:
Kindly sponsored by Sarstedt Ltd.
Produced by:
Pathology Laboratory
The Ipswich Hospital NHS Trust
Heath Road, Ipswich, Suffolk IP4 5PD
Direct tel: 01473 703721
Hospital switchboard: 01473 712233
www.ipswichhospital.nhs.uk
January 2012
© The Ipswich Hospital NHS Trust, 2006-2012. All rights reserved.
Not to be reproduced in whole, or in part, without the permission of the copyright owner.
DPS ref: 13197-11.indd

2012 Sample Requirements Pathology Laboratory Guide

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