MS-PAK NEWSLETTER
Transcription
MS-PAK NEWSLETTER
Volume 04 (May-August 2014) MS-PAK NEWSLETTER MS-PAK is a Public Service Initiative of the MS-PAK is a Public Service Initiative of the International Center for Chemical and Biological Sciences (ICCBS), International Center for Chemical and Biological Sciences (ICCBS) University of Karachi University of Karachi Golden Jubilee ICCBS 5 Decades of Excellence and leadership in science (1966-2016) Contents: 1. Team MS-PAK Newsletter 2. MS-PAK Member’s Profile 3. Welcome note from the desk of Coordinator 4. Mass Spectrometry Facilities at the ICCBS 5. Mass Spectrometric Books Available at the National Library of Chemical Sciences 6. Mass Spectrometry Links (Societies and Journals) 7. Latest Technology Advances in Mass Spectrometry 8. Upcoming Events Related to Mass Spectrometry 9. How Far We can Go with Mass Spectrometry 10. Mass Spectrometry Team in Pakistan Patron: Prof. Dr. Atta-ur-Rahman (FRS., N.I., H.I., S.I., T.I.) Editor-In-Chief: Prof. Dr. M. Iqbal Choudhary (H.I., S.I., T.I.) Editor & Coordinator : Dr. Syed Ghulam Musharraf Assistants to Editor & Coordinator: Qamar ul Arfeen and Dilshad Hussain 1 Volume 04 (May-August 2014) Profile of Team of MS-PAK Newsletter Prof. Dr. Atta-ur-Rahman (N.I., H.I., S.I., T.I.) Fellow of the Royal Society (FRS) Ph.D. (Cambridge) Sc.D. (Cambridge) D.Sc. (Sir Syed Engineering University, Karachi University, Gomal University) UNESCO Science Laureate Patron-in-Chief, ICCBS Prof. Dr. Atta-ur-Rahman has over 940 research publications in top international journals, 28 patents and 150 books written or edited, published and circulated internationally. He is also the Editor-in Chief of many European science journals. His research interests include synthesis of natural products, chemistry of bioactive secondary metabolites, development and use of modern NMR spectroscopic methods, search for new enzyme inhibitors and anti-oxidants as well as biotransformation of bioactive compounds. Prof. Dr. M. Iqbal Choudhary (H.I., S.I., T.I.) Distinguished National Professor Ph.D. (University of Karachi) Sc.D. (University of Karachi) Director, ICCBS Prof. Dr. M. Iqbal Choudhary has over 890 research publications in top international journals with cumulative impact factor of over 1600 and 7,500 citations, 36 national and international patents and 32 books published and circulated internationally. He is also the Editor-in-Chief of 4 European science journals and book series. His current research interests include search for new and effective enzyme inhibitors, antioxidant and antiangiogenic agents and anti-parasitic compounds. Dr. Syed Ghulam Musharraf Ph. D. (H.E.J. Research Institute of Chemistry, University of Karachi) Associate Professor E-mail:musharraf1977@ yahoo.com Telephone: 111-222-292 Ext:134 Office: Room HEJ-308 Dr. Musharraf has over 70 research publications in reputed international journals. His current research is based on the utilization of modern mass spectrometric approaches as a powerful analytical tool in (1) proteomics and metabolomics studies for the search of bio-signatures in lung, oral and breast cancers which are common cancers in Pakistan, (2) screening and optimization of algal oil in Pakistani algae for biodiesel production, (3) development of protonated / deprotonated fragmentation pathways and structurefragmentation relationship (SFR) of pharmacologically significant classes of natural products, (4) MALDI matrix development for the analysis of metabolites in biological fluids and (5) LC- and GC-MS method development for marker compound analysis in Pakistani agricultural products and commercially important plant extracts. 2 Volume 04 (May-August 2014) Welcome Note From the Desk of Coordinators MS-PAK is an initiative of the International Center for Chemical and Biological Sciences (ICCBS), University of Karachi to build national capacity in a versatile and emerging field of Mass Spectrometry. This field has been one of the most demanded areas in research world. Few of the most recent applications of Mass Spectrometry are mostly oriented towards biochemical problems, such as proteome, metabolome, high throughput drug discovery and metabolism. Other analytical applications are routinely applied in pollution control, food control, forensic science, natural products or process monitoring and many others. Over last one decade our nation has invested considerable amount of financial resources on the procurement of most modern Mass Spectrometric tools. However, many of these machines are not effectively utilized to say the least. This is certainly a gross national loss which financially constrained country like ours cannot afford. Through building national capacity in the effective use of Mass Spectrometry, technically and scientifically, we can contribute immensely in various disciplines. MS-PAK is a first step forward towards that direction. Please be our partner in this journey! 3 Volume 04 (May-August 2014) Mass Spectrometry Facilities at the ICCBS Mass Spectrometric Technique Available at ICCBS; GC-QQQ-MS/MS LC-ESI-MS/MS LC-QQ-MS 1. 2. 3. 4. 5. 6. 7. Electron Impact (EI) Chemical Ionization (CI) Fast Atomic Bombardment (FAB) Atmospheric Pressure Chemical Ionization (APCI) Electrospray Ionization (ESI) Matrix Assisted Laser Desorption Ionization (MALDI) Inductively Coupled Plasma (ICP) Mass Spectrometers Installed at ICCBS; 1. GC/MS/MS (QQQ) [Agilent 7000A] 2. MAT 95 XP [Thermo Finnigan] 3. JMS-HX110 [JEOL] 4. JMS-600H-OLD [JEOL] 5. JMS-600H-NEW [JEOL] 6. MAT 312 [Varian] 7. API 150 EX LCMS [AB SCIEX] 8. LC-QTOF MS/MS System [AB SCIEX] 9. MALDI TOF/TOF Ultraflex III [Bruker] 10. LC/MS/MS (QQQ) [Agilent] MALDI- TOF/TOF-MS JEOL JMS 600-H MAT 95 XP Latest Arrivals; LCT-Premier 1. 2. 3. 4. 5. LC-Tandem Quadrupole LCT- Premier QTOF- Micromass Quattro-Ultima ICP-MS JEOL JMS-HX110 Database Resources; QTOF-Micromass 1. Mascot Search Engine 2. Wiley Registry 9th/NIST Library (700,000 Mass Spectra) Quattro Ultima Sample Submission Link; http://www.iccs.edu/mass/samplesubmission.php 4 Volume 04 (May-August 2014) Mass Spectrometric Books Available at the National Library of Chemical Sciences S.No. 1. 2. 3. 4. 5. Title A Beginner's Guide to Mass Spectral interpretation Analysis of Essential Oils by Gas Chromatography and Mass Spectrometry Applications of Mass Spectrometry to Organic chemistry Applications of Mass Spectrometry to Organic Stereochemistry Applied Electrospray Mass Spectrometry Authors Published by Lee, Terrence A Wiley, (Chichester, New York) Masada, Yoshir Wiley, (New York ) Reed, Rowland Ivor Academic Press, (London, New York) Splitter, Janet S. VCH, (New York ) Ganguly, A. K. Gross, M. L, Pramanik, B. Waller, George R. Marcel Dekker, (New York) 6. Biochemical Applications of Mass Spectrometry 7. Chemical Ionization Mass Spectrometry Harrison, Alex. G CRC Press, (Boca Raton, Fla.) 8. Computational Methods for Mass Spectrometry Proteomics Computers in Mass Spectrometry Current Practice of GC-MS Dictionary of Mass Spectrometry Eight Peak Index of Mass Spectra Vol. 2 Eight Peak Index of Mass Spectra Vol. 3 Part 1 Eight Peak Index of Mass Spectra Vol. 3 Part 2 Electrospray and MALDI Mass Spectrometry Experimental Mass Spectrometry Fundamentals of Contemporary Mass Spectrometry GC/MS Handbook of Analytical Derivatization Reactions Handbook of GC/MS Indolalkaloide. Inductively Coupled Plasma Mass Spectrometry Inorganic Mass Spectrometry Interpretation of Mass Spectra Eidhammer, Ingvar. Chapman, J. R. Niessen, W. M. A. Mallet, A. I - John Wiley & Sons, (Chichester, England, Hoboken, NJ ) Academic Press, (London, NewYork ) M. Dekker, (New York) Wiley, (Chichester, Hoboken, NJ ) Mass Spectrometry Data Center Mass Spectrometry Data Center - Mass Spectrometry Data Center Cole, Richard B Wiley, (Hoboken, N.J.) Russell, David H Dass, Chhabil Plenum Press, (New York ) Wiley-Interscience, McMaster, Christopher. Knapp, Daniel R. Wiley, (New York ) Wiley, (New York ) Hubschmann, H.J. Hesse, Manfred Taylor, Howard E. Wiley-VCH, (Weinheim) Verlag Chemie (Weinheim/Bergst) Academic Press, (San Diego) Becker, Johanna Sabine. McLafferty, Fred W. John Wiley & Sons University Science Books, (Calif.) 9. 10. 11. 12. 13. 14. 15. 16. 17. 18. 19. 20. 21. 22. 23. 24. Wiley, (New York ) 5 Volume 04 (May-August 2014) 25. 26. 27. 28. 29. 30. 31. 32. 33. 34. 35. 36. 37. 38. 39. 40. 41. 42. 43. 44. 45. 46. 47. 48. 49. 50. 51. Interpretation of Mass Spectra of Organic Compounds Interpreting Protein Mass Spectra Introduction to Organic Mass Spectrometry Introduction to Mass Spectrometry IUPAC Mass Spectrometry of Natural Products-11 (Vol. 50 Issue 3) LC/MS Liquid chromatography-mass spectrometry Liquid Chromatography Mass Spectrometry Mass and Abundance Tables for Use in Mass Spectrometry Mass Spectrometry Mass Spectrometry Basics Mass Spectrometry for Biotechnology Mass Spectrometry for Chemists and Biochemists Mass Spectrometry in Medicinal Chemistry Mass Spectrometry in the Biological Sciences Mass spectrometry of Inorganic and Organometallic Compounds Mass Spectrometry of Organic Compounds Mass Spectrometry Mass Spectrometry/Mass Spectrometry Mass Spectrometry: A Specialist Periodical Report Vol. 3 Mass Spectrometry: A Specialist Periodical Report Vol. 4 Mass Spectrometry: A Specialist Periodical Report Vol. 5 Mass Spectrometry: A Specialist Periodical Report Vol. 6 Mass Spectrometry: A Specialist Periodical Report Vol. 7 Mass Spectrometry: A Specialist Periodical Report Vol. 8 Mass Spectrometry: A Specialist Periodical Report Vol. 9 Mass Spectrometry: A Specialist Periodical Foster, Norman G. Academic Press, (New York,) Snyder, A. Peter, Anees A. Siddiqui Watson, J. Throck. B. Sklarz Hoffmann, Edmond de. Johnstone, R. A. W Siuzdak, Gary. Johnstone, R. A. W. Oxford University, (New York ) CBS Publishers, Distributors (India) Lippincott-Raven, (Philadelphia) Germany (Bulgarian Academy of Sciences) John Wiley, (Hoboken, N.J.) J. Wiley, (New York) M. Dekker, (New York) Elsevier Pub. Co., (Amsterdam, | New York) J. Wiley, (West Sussex, England) CRC Press, (Boca Raton) Academic Press, (San Diego, Calif.) Cambridge University Press Klaus T. Wanner Burlingame, A. L. Wiley-VCH Verlag GmbH & Co. Humana Press, (Totowa, N.J.) Spalding, T. R. Elsevier Scientific Pub. Co., (Amsterdam, New York,) Holden-Day (San Francisco) John Wiley & Sons, (New York ) VCH Publishers, (New York, N.Y.) The Chemical Society Burlington House (London) The Chemical Society Burlington House (London) The Chemical Society Burlington House (London) The Chemical Society Burlington House (London) The Chemical Society Burlington House (London) The Chemical Society Burlington House (London) The Chemical Society Burlington House (London) The Chemical Societry (London) McMaster, Marvin C. Ardrey, R. E. Niessen, W. M. A. Williams, A. E Djerassi, Carl Barker, James, Glish, Gary L. R.A.W. Johnstone R.A.W. Johnstone R.A.W. Johnstone R.A.W. Johnstone R.A.W. Johnstone M.E. Rose M.E. Rose D.H. Williams 6 Volume 04 (May-August 2014) 52. 53. 54. 55. 56. 57. 58. 59. 60. 61. 62. 63. 64. 65. 66. 67. 68. 69. 70. 71. 72. 73. 74. Report Mass Spectroscopy Massenspektrometrie Metastable Ions. Methods in Enzymology : Mass Spectrometry Vol. 193 Modern Mass Spectrometry: Topics in Current Chemistry Vol. 225 Modren Methods Of Plant Analysis: Gas Chromatography/Mass Spectrometry Vol.3 Practical Organic Mass Spectrometry Principles and Practice of Biological Mass Spectrometry Quadrupole Storage Mass Spectrometry Quantitative Applications of Mass Spectrometry Quantitative Proteomics by Mass Spectrometry Quantitative Mass Spectrometry Quantitative Mass Spectrometry in Life Sciences Structure Elucidation of Natural Products by Mass Spectrometry Tables for Use in High Resolution Mass Spectrometry Techniques of Combined Gas Chromatography/Mass Spectrometry Textbook for Mass Spectrometry The Important Peak Index of the Registry of Mass Spectral Data Vol. 1 The Important Peak Index of the Registry of Mass Spectral Data Vol. 2 The Important Peak Index of the Registry of Mass Spectral Data Vol. 3 The Mass Spectra of Organic Molecules Trace Quantitative Analysis by Mass Spectrometry Using Mass Spectrometry for Drug Metabolism Studies Barber, R. C. Budzikiewicz, Herbert. Cooks, R. G. James A. McCloskey Cambridge University Press Verlag Chemie Elsevier Scientific Pub. Co. Academic Press, Inc. (New York) Christoph A. Schalley Springer-Verlag (New York) H.F. Linskens Springer Private Limited (New Delhi) Chapman, J. R. Dass, Chhabil. Wiley, (Chichester) John Wiley, (New York) Hughes, Richard J. Lavagnini, Irma. Wiley, (New York ) Wiley, (Chichester) Sechi, Salvatore, Humana Press, (Totowa, N.J.) Millard, Brian J. Leenheer, A. P. de. Heyden, (London) Elsevier Scientific Pub. Co. Djerassi, Carl Holden-Day, (San Francisco,) Cleaver, Raymond Leslie McFadden, William H. Heyden in co-operation with Sadtler Research Laboratories Wiley (New York,) Fred W. Mclafferty Jeol Ltd MS Section, Training Center John Wiley & Sons, Inc (New York) Fred W. Mclafferty John Wiley & Sons, Inc (New York) Fred W. Mclafferty John Wiley & Sons, Inc (New York) Saunders, R. A. Basic, Cecilia. Elsevier Pub. Co. John Wiley & Sons Korfmacher, Walter A. CRC Press, (Boca Raton, FL) Note: You can also search your required mass spectrometry book by new advanced Online Public Access Catalog (OPAC) of ICCBS Libraries (library.iccs.edu) 7 Volume 04 (May-August 2014) Mass Spectrometry Links Mass Spectrometry Journals Mass Spectrometry Reviews Journal of the American Society for Mass Spectrometry Rapid Communications in Mass Spectrometry Journal of Mass Spectrometry European Journal of Mass Spectrometry International Journal of Mass Spectrometry Advances in Mass Spectrometry Biological Mass Spectrometry Biomedical & Environmental Mass Spectrometry Biomedical Mass Spectroscopy Organic Mass Spectrometry Mass Spectroscopy Societies American Society for Mass Spectrometry (ASMS) Argentinean Society for Mass Spectrometry (SAEM) Australian and New Zealand Society for Mass Spectrometry (ANZSMS) Brazilian Mass Spectrometry Society (BrMASS) British Mass Spectrometry Society (BMSS) Canadian Society for Mass Spectrometry (CSMS) Chinese Mass Spectrometry Society Dutch Society for Mass Spectrometry (NVMS) European Society for Mass Spectrometry (ESMS) French Society of Mass Spectrometry (SFSM) German Mass Spectrometry Society (DGMS) Indian Society for Mass Spectrometry (ISMAS) International Mass Spectrometry Foundation (IMSF) Mass Spectrometry Society of Japan (MSSJ) Russian Mass Spectrometry Society Singapore Society for Mass Spectrometry Spanish Society for Mass Spectrometry (SEEM) Swedish Mass Spectrometry Society (SMSS) Swiss Group for Mass Spectrometry (SGMS) Latest Technology Developments in Mass Spectrometry LC-IT-TOF-MS: The LCMS-IT-TOF is a new type of mass spectrometer that combines QIT (ion trap) and TOF (time-of-flight) technologies; high Resolution (>12,000) and with capability to perform MSn (up to MS10). MALDI-QIT-TOF-MS: The unique combination of MALDI and QIT allows generation of ions using a number of different matrices; switching between positive and negative ionization modes in seconds; simple high resolution precursor ion selection for MSn experiments. Incorporating a TOF analyzer promotes high resolution and high mass accuracy for all ions. Imaging Mass Microscope: A combination of an optical microscope which allows the observation of high-resolution morphological images, with a mass spectrometer which identifies and visualizes the distribution of specific molecules; superimposing the two images obtained based on these very different principles, has created a significant new research tool, the imaging mass microscope. The accurate and high resolution mass images from the iMScope will drive your research to the next level. LTQ-Orbitrap Hybrid MS: High mass accuracy lowers false positive rates. Parallel acquisition mode optimizes productivity. High MS and MS/MS sensitivity, wide dynamic range and fast cycle times increase protein identification, leads to high confidence de novo sequencing results. To enhance the flexibility of fragmentation experiments for advanced proteomics and small molecule research, the instrument features an HCD collision cell. 8 Volume 04 (May-August 2014) Upcoming Events Related to Mass Spectrometry “Clinical Applications of Mass Spectrometry” 29-30 October 2014 (Barcelona, Spain) With the ability to measure multiple analytes with high sensitivity, often faster and more cheaply than other methods, mass spectrometry is becoming an attractive method of analysis for the clinic. Featuring an array of leading researchers and clinicians, SELECTBIO’s Clinical Applications of Mass Spectrometry conference aims to provide you with an insight into the latest developments in this area. As the analytical power of mass spec is realized, the range of applications using this technology continues to expand. Focus at this meeting will be given to both traditional & emerging uses of MS in the clinic. Hot topics to be covered include developments of MS in applications ranging from vitamin D detection to newborn blood spot analysis. Attending this event will provide you with excellent opportunities for networking with like minded peers, helping you to build new relationships and optimize your workflow. Running alongside the conference will be an exhibition covering the latest technological advances and associated services from leading solution providers within this field. Registered delegates will also have access to the co-located Food Analysis Congress, ensuring a cost effective trip. Poster Submission Deadline: 13 October 2014 Web URL: http://selectbiosciences.com/conferences/index.aspx?conf=CAMS2014 9 Volume 04 (May-August 2014) How Far We Can Go With Mass Spectrometry!!! 1. Imaging Mass Spectrometry in Clinic Optical microscopes cannot determine which molecules are localized in the region of interest. On the other hand, the positional information of molecules is lost in mass spectrometric analysis. Imaging mass spectrometry directly detects both natural and synthetic molecules in tissue sections and measures mass spectra, while retaining their positional information associated with the tissue section. Then, two-dimensional distributions of specific molecules are visualized by combining the positional information of each mass spectrum and the signal intensity for specific ions in the mass spectrum (MS imaging). From a clinical perspective, MS imaging – unlike most imaging methods – removes the need for target-specific labeling whilst measuring the required broad spectrum of endogenous and exogenous analytes in tissues within the histological context. The highly multiplexed analyses offered by MS imaging enables not only diagnostic (targeted) assays but also the potential for biomedical discovery applications. Multi-class analysis capability (from proteins and peptides to drugs, lipids and beyond) is a further strength of MS imaging. The practical simplicity of MS imaging and its ability to gain reliable information, even from the smallest tissue samples, means that it has the potential to complement traditional histopathologic evaluation for assisting in diagnosis, risk assessment, or response prediction to therapy. 10 Volume 04 (May-August 2014) MassSpectrometry SpectrometryininSpace Space 2.2.Mass The Ion and Neutral Mass Spectrometer (INMS) is intended to measure positive ion and neutral species composition and structure in the upper atmosphere of Titan and magnetosphere of Saturn, and to measure the positive ion and neutral environments of Saturn's icy satellites and rings. Main objectives of INMS are to measure ion and neutral species composition and structure in the upper atmosphere of Titan; to study Titan chemistry; to investigate(INMS) the interaction of to Titan upperpositive atmosphere with the Theatmospheric Ion and Neutral Mass Spectrometer is intended measure ion and neutral magnetosphere and solarand windstructure and to measure ion and neutral species compositions during ring species composition in the upper atmosphere of Titan and magnetosphere of plane crossings and icy satellite flybys. Saturn, and to measure the positive ion and neutral environments of Saturn's icy satellites and rings. INMS Sensing Instruments is a closed source (neutrals only) and open source (ion and neutrals) withMain a Lens System of andINMS Quadrupole Mass Analyzer Detector (1-99 amu). and structure in the objectives are to measure ion and and neutral species composition upper atmosphere of Titan, to study Titan atmospheric chemistry, to investigate the interaction of The Ion and Neutral Mass Spectrometer Subsystem (INMS) will be used to study the neutral gases Titan upper atmosphere with the magnetosphere and solar wind and to measure ion and neutral and positive ions in the atmospheres of Saturn and Titan and gases in the vicinities of the Saturnian species compositions during ring plane crossings and icy satellite flybys. rings and the icy satellites. It will also study the magnetosphere of Saturn. INMS Sensing Instruments is a closed source (neutrals only) and open source (ion and neutrals) The major functional components of the INMS Subsystem are an open ion source, a closed ion with a Lens System and Quadrupole Mass Analyzer and Detector (1-99 amu). source, a Quadrupole deflector and lens system, a Quadrupole mass analyzer, and a dual detector system. theseSpectrometer components,Subsystem click on their names.will be used to study the neutral The For Ion information and Neutral on Mass (INMS) gases and positive ions in the atmospheres of Saturn and Titan and gases in the vicinities of the Saturnian rings and the icy satellites. It will also study the magnetosphere of Saturn. The major functional components of the INMS Subsystem are an open ion source, a closed ion source, a Quadrupole deflector and lens system, a Quadrupole mass analyzer, and a dual 11 Volume 04 (May-August 2014) MS-PAK User Group Member’s Profile S.No. Name Degree Affiliation Research Interest Email 1 Aisha Khalid M.Sc HEJRIC, UoK Apply MALDI technique to study enzyme kinetics snoopy.ash@hotmail.com 2 Amna Siddiqui M.Sc HEJRIC, UoK Working on the metabolomics of serum and plasma jabbar_siddiqui08@yahoo.com 3 Arslan Ali M.Sc HEJRIC , UoK LC-MS/MS and MALDI-TOF analysis of natural product arslanali1986@gmail.com 4 Ayesha Iqbal B.S. PCMD, UoK Working on proteomics in mass spectrometry ayesha.26@live.com 5 Dilshad Hussain M.Phil BZU Multan Fabrication of hydrophobic materials and their application in proteomics shad3233@yahoo.com 6 Fahmida Jabeen M.Phil BZU Multan Analysis of peptides and proteins by self-fabricated hydrophillic materials fahmida_09_07@hotmail.com 7 Fatima Batool M.Phil BZU Multan Analysis of biological fluids using MALDI matrices fatima_biochem@yahoo.com 8 Kausar Shah B.S. HEJRIC UoK LC-MS/MS and MALDI-TOF analysis of natural product syed_jarrar2012@hotmail.Com 9 Madiha Goher M.Sc HEJRIC UoK Fragmentation patern studies of natural products mgoher2008@yahoo.com 10 Mahwish Saleem M.Sc PCMD, UoK Peptide profiling mahwishsaleem50@yahoo.com 11 Muhammad Arif Ahmed M.Sc HEJRIC UoK GC-MS analysis of microalgal oil for biodiesel production arif2184@gmail.com 12 Muhammad Imran M.Sc BZU Multan Interpretation of data using MS and MS/MS for complex biological samples - 13 Muhammad Irfan Akram M.Sc HEJRIC UoK Metabolite profiling of aromatic rice variation irfee_ku@yahoo.com 14 Muhammad Salman Bhatti BS HEJRIC UoK Working on MALDI-MS for analysis of proteins and enzymes dr.salmanbhatti@gmail.com 15 Naghma Hashmi Ph.D HEJRIC, Uok Bioequivalence study of drugs by LC-MS naghmahashmi@yahoo.com 16 Najia Shahid BS HEJRIC UoK Development of New MALDI matrices for the analysis of metabolites najias_2@yahoo.com 17 Naureen Siddiqui M.Sc HEJRIC UoK Compounds have been synthesized and confirmed by mass spectyroscopy naureen_siddiqui22@yahoo.com 18 Nayab Kanwal M.Sc HEJRIC UoK Chemical fingerprinting and standardization of some medicinal plants nayab_kan@yahoo.com 19 Qamar ul Arfeen M.Sc HEJRIC UoK Characterization of degradation products and metabolites of steroidal drugs quaish_85@yahoo.com 20 Sajda Ashraf M.Sc HEJRIC UoK Characterization of isolated compounds from plants sajda.ashraf@yahoo.com 21 Shaheen Shaukat M.Sc ICCBS, UoK Working as a operator in mass spectrometry lab shaheen_shaukat@yahoo.com 22 Shumaila Mazhar M.Sc HEJRIC UoK Metabolite profiling in order to find out biomarker compound for lung cancer shumaila_chm93@yahoo.com 23 Syeda Ambreen Fatima M.Sc HEJRIC UoK Analysis of environmental samples by mass spectrometry chemically_genius@hotmail.com 24 Zareena Bibi M.Sc HEJRIC UoK Fragmentation patern studies of natural products zarina_ku@yahoo.com 25 Faraz ul Haq M.Sc HEJRIC UoK Analysis of natural products faraz_uhq1@hotmail.com 26 Ina Faraz M.Sc HEJRIC UoK Analysis of natural products ina_faraz@hotmail.com 27 Fareeha M.Sc HEJRIC UoK Analysis of natural products Fariesidd2@gmail.com 28 Rabia M.Sc HEJRIC UoK Analysis of natural products Biatariq-21@hotmail.com 29 Sabiha M.Sc HEJRIC UoK Analysis of natural products lubabakhan@gmail.com Volume 04 (May-August 2014) 13