e table S1. Supplemental material Yang et al.,
Transcription
e table S1. Supplemental material Yang et al.,
Supplemental material Yang et al., http://www.jem.org/cgi/content/full/jem.20140283/DC1 Table S1. Primers for real-time PCR analysis and Hrd1 mutant mice genotyping The Jour nal of Exper imental Medicine Gene name Hrd1 BLIMP1 MHC-I MHC-II CIITA -Actin Genotyping Hrd1 targeted mutation JEM Primer sequences Forward, 5-CTGGGTATCCTGGACTTCCTC-3; and reverse, 5-AAGCACCATGGTCATCAGAA-3 Forward, 5-ATGGAGGACGCTGATATGC-3; and reverse, 5-CCTTACTTACCACGCCAATAAC-3 Forward, 5-AAGTGTCTGATGTTCCCTGTG-3; and reverse, 5-ATGTCCCTCAGTGTTTGGC-3 Forward, 5-GAGC ATCCCAGCCTGAAGA-3; and reverse, 5-CGATGCCG CT CAACATCTT-3 Forward, 5-CACCCCCAGATGTGTATGTGCT-3; and reverse, 5-ACGAGGTTTCCCAGTCCAGAA-3 Forward, 5-TGGCGCTTTTGACTCAGGAT-3; and reverse, 5-GGGATGTTTGCTCCAACCAA-3 Forward, 5-ACACGTCTCCTGGGTGATCTAC-3; and reverse, 5-CTTCTGTGCAGCTGGTATTTGG-3 S1