ZEOsphere® - Zeochem AG

Separation Technologies: our work – our life – our passion.
ZEOsphere®
Chromatography Gels
Separation Solutions
· Large Scale Batches
· Professional Support from Concept to Fullscale
· Customized Derivatization
ZEOsphere® - High Performance Separation
C-Gels
High Quality
Swiss Production
Zeochem AG, a manufacturer of
high quality spherical and irregular
silica gels and molecular sieves,
was established more than 190 years
ago and is still based at the original
location on Lake Zurich, Switzerland.
Zeochem is a subsidiary of the Swiss
CPH Chemie+Papier Holding AG.
SEM of ZEOsphere® 100 15 µm
With ZEOsphere®, a series of spherical
silica gels, we are the only Swiss
company offering these products.
Additionally, Zeochem is one of the
few worldwide fully back-integrated
silica producers, controlling the quality
of the product right from the high
purity water glass up to the fully
end-capped bonded high performance
gel, available in both normal and
reversed phase.
ZEOsphere® silica gels are produced
by a large batch scale emulsion
process providing reproducible
chromatographic separations of
high quality. The efficiency of liquid
chromatography relies on narrow
particle size distributions, very high
mechanical stability and a high specific
surface area to deliver consistent and
reliable results. We are constantly
working on improving this process
with one main goal:
Absolute uniformity between batches.
ZEOsphere® - Technical Information
Properties of ZEOsphere® compared to Competitors
ZEOsphere®
Competitor 1
Competitor 2
Specific surface area [m2/g]
Property
303
339
291
Pore volume [ml/g]
0.84
0.90
0.75
Pore size [Å]
110
107
103
d(50) [μm]
15.4
15.0
15.0
d(90) / d(10) [-]
1.61
1.58
1.66
Residual water content [wt%]
0.9
3.1
2.0
pH [-]
5.4
5.0
5.7
Electric conductivity [μS/cm]
3
15
3
Na[ppm]
31
34
110
Fe[ppm]
<1
2
<1
Al[ppm]
3
2
8
K[ppm]
5
3
4
Ca[ppm]
39
38
36
Mg[ppm]
7
8
8
Pb[ppm]
< 0.1
< 0.1
< 0.1
85
87
166
Total metal content [ppm]
Based on Properties of ZEOsphere® 100 15 µm
ZEOsphere® C18
Competitor 1
Competitor 2
d(50) [μm]
Property
15.4
15.4
14.1
d(90) / d(10) [-]
1.61
1.54
1.51
Carbon content [wt%]
20.3
19.9
15.5
Based on Properties of ZEOsphere® 100 C18 15 µm
Spherical Precision
SEM of ZEOsphere® 100 15 µm
The present product range offers pore
sizes from 70 to 100 Å and particle
sizes from 5 μm up to 40 μm. Other
speciality products can be manufactured according to customers‘ specific
requirements. Our surface modification
technology enables the bonding of all
existing silica gel products regardless
of shape, pore and particle size.
With our products there are no
headaches concerning the scale
of your process; the quality of
ZEOsphere® ensures seamless transitions from analytical, semi-prep and
prep to large scale industrial chromatography, as we are providing the same
silica specifications with appropriate
particle size distributions.
ZEOsphere® can be delivered in
2.5 l polyethylene bottles or 25 kg
resealable plastic drums.
Test Data
Antidepressants Separation
Insulin, Desamido Insulin Separation
180
65
160
ZEOsphere® C18
Competitor 1
45
120
UV Signal at 215 nm
UV Signal at 254 nm
140
ZEOsphere® C18
Competitor 1
55
100
80
60
35
25
15
40
5
20
-5
0
10
20
30
40
50
Time [min]
Test molecules: nortriptyline, amitriptyline, imipramine; mobile phase:
MeOH: 25 mM (NH4)H2PO4, 72:28 (w:w), pH=6.3; temperature: 25°C.
0
10
20
30
40
Time [min]
Test molecules: insulin, desamido insulin; mobile phase: EtOH: 50 mM (NH4)2SO4, pH 3.5,
28.3:71.7 (w:w); temperature: 25°C.
50
Raw Peptide Mixture
195
ZEOsphere® C18
Competitor 1
175
UV Signal at 220 nm
155
30
ZEOsphere® C18
Competitor 1
25
20
135
15
115
10
95
5
75
0
55
-5
15
35
20
15
-5
0
10
20
30
Time [min]
40
50
60
25
Time [min]
30
35
40
Test mixture: raw 3kDa peptide; buffer A: ACN : 50 mM NaH2PO4: H3PO4, 10.4:89.4:0.2
(w:w:w), buffer B: ACN : 50 mM NaH2PO4: H3PO4, 52.2:47.6:0.2 (w:w:w), gradient from
1% buffer B to 100% buffer B in 30 minutes; temperature: 25°C.
Advantages
Availability of standard modifications from stock
Large batch sizes (180 kg / batch)
High carbon load reproducibility
Monofunctional bonding technology
Standard particle sizes from 5 – 40 μm (further sizes available
upon request)
C18, C8, NH², CN, SH, Diol, SAX (further derivatizations available upon request)
The base material control combined with the proprietary bonding technique make
ZEOsphere® the material of choice for all separation processes where high resolution,
good peak shape, short elution time are demanded, even at elevated pH values.
Major Applications
Peptides
API
Small Proteins
Insulin
Natural Products
Antidepressants
Vitamins
Amines
Steroids
ZEOsphere® - Comprehensive Solutions
ZEOsphere® displays very uniform
perfectly spherical particles, which
provide an extremely pressure stable
silica gel, free of fines, allowing
reproducible column packing with
optimized backpressure. The low
metal ion content of ZEOsphere®
is achieved during our multistep
production process. Consequently,
metal ion solute interactions are
minimized providing symmetrical
peaks with little or no tailing.
The homogenous surface properties
ensure the highest performance
with even the most challenging
substrates.
Cost is a major driver for all pharmaceutical and chemical companies. The
purification stage influences the total
separation cost significantly. The efficiency and cost of the silica gel is in
the focus of today‘s R & D departments
and Zeochem offers premium products
with competitive prices, supporting
our customers by reducing their spend
and increasing their yields.
Technical Support
Zeochem prides itself in providing
a second to none service to our
customers. Our chemical specialists
and the technical sales team utilize
an in-house developed software
based modelling tool to design the
optimum process prior to upscaling
from the lab. Often a short discussion
with our team is sufficient to
provide a comprehensive solution
to our customer base.
www.zeochem.com
Zeochem AG
Seestrasse 108 · 8707 Uetikon am See · Switzerland
Phone +41 44 922 93 93 · Fax +41 44 920 20 93
info@zeochem.ch · www.zeochem.com
Zeochem L.L.C.
1600 West Hill Street · Louisville, KY 40210 · USA
Phone +1 502 634 7600 · Fax +1 502 634 8133
info@zeochem.com · www.zeochem.com
For your local agent or distributor please go to
www.zeochem.com
A Company of
Chemie + Papier Holding AG