Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp

Aromatic Hydrocarbon Utilization Ability of Chromohalobacter sp.
Kıymet GÜVEN1, Sevim Feyza ERDOĞMUŞ2, Safiye Elif KORCAN3,*, Muhsin KONUK4,
Mehmet Burçin MUTLU1
1
2
Anadolu University, Faculty of Science, biology department, 26100-Eskişehir, Turkey.
Department of Laboratory and Veterinary Health, Technical Vocational School of Higher
Education of Bayat, Afyon Kocatepe University, 03780, Afyonkarahisar, Turkey.
3
Afyon Kocatepe University, Faculty of Science and Literatures, Biology Department, 03200-
Afyonkarahisar, Turkey.
4
Üsküdar University, Faculty of engineering and Natural Sciences, Molecular Biology and
Genetics department, 34662-Istanbul, Turkey.
Abstract
This study aimed to reveal the ability of utilizing different aromatic hydrocarbons (phydroxybenzoic acid, naphthalene, phenanthrene, pyrene) by a halotolerant bacterial strain
Chromohalobacter sp. under saline conditions. The aromatic hydrocarbon degradation pathways
were identified. PCR amplification was carried to define the gene zones which codify dioxygenases
of the isolates. Possible gene zones of catechol 1,2 dioxygenase and protocatechuate 3,4
dioxygenase were determined. According to PCR amplification and enzyme test results
Chromohalobacter sp. utilizes aromatic hydrocarbons by the ortho cleavage of the β-ketoadipate
pathway. In this study, it was concluded that this isolate could be used in bioremediation studies of
the saline environments contaminated with aromatic hydrocarbons.
Key words: Chromohalobacter sp., catechol 1,2 dioxygenase, protocatechuate 3,4 dioxygenase.