European Multi Lake Survey Summer 2015 CyanoCOST/ NETLAKE

European Multi Lake Survey
Summer 2015
CyanoCOST/ NETLAKE Training School
May 2015
Organization: Evanthia Mantzouki, Bastiaan Ibelings
Participants: CyanoCOST & NETLAKE
Sevilla, CYANOCOST
19/02/2015
Aims of European Multi Lake Survey
1. Quantify the relative role of nutrients (N vs. P) and
temperature as drivers of cyanoblooms (mode and type
interaction)
2. Assess the function of trophic state, basin type, functional
traits of dominant taxa
3. Risk analysis , toxin profiles of all lakes
27 Countries
(54% Inclusiveness)
55 Participants
≈
100s lakes
PROTOCOLS
• EPA – National Lakes Assessment 2012
• GLEON – TTG Spring Blitz 2013
LAKE SELECTION (NLA Criteria)
≥ 1 ha in total surface area
≥ 1000 square meters of open water
≥ 1 meter in depth
• Not saline (due to salt water intrusion or tidal influence)
• Not used for aquaculture
#
Site ID
Lake Name
Country/ Region
Stratification
Regime
Trophic
State
Latitude
Climate
Lake
Size
Sediment
Type
Contact
Meteorological data (nearest station)
Wind speed
Air Temperature (°C)
Precipitation (daily mm per year)
2. Scum
Chlorophyll a
Toxins
Phytoplankton
1. Historical or Deepest Point
T, C profiles
Chl fluorescence / Secchi depth
DO, pH, PAR
P, N
Phytoplankton (microscopy, flowcytometry)
Micro-, Mesozooplankton
Microbes, community composition (preserve for future)
Toxins
Field Scenario
1. Prepare equipment and supplies for sampling. Calibrate sensors and multi probe meter
2. Lake sampling (Sampling day chosen by each participant, capturing the seasonal maximum)
Meteorological data: Wind speed, air temperature and precipitation
Profiles of temperature, Chl Fluorescence, DO and pH in Index site
Secchi depth
3. Determine depth of sampling (3 depths)
Sampling at 3 discreet depths: Chlorophyll a, nutrients, phytoplankton, zooplankton,
microbes- community composition, toxins
4. Inspect periphery and spot Scum
Sampling surface: Chlorophyll a, phytoplankton, toxins
5. Ensure all samples are labelled and dated correctly
6. Sample preparation
7. Sample analysis
8. Shipping
CYANOCOST and NETLAKE Training School 2015
From A to Z: Methods for a successful Multi-lake survey on cyanobacterial blooms
Evian Les Bains, France
10-14/May / 2015
Tentative programme
• Webinar (April) – Feedback, Protocols, EMLS Preparation needs
• Training School
• 1st day:
•
•
•
•
•
Phytoplankton (B. Ibelings, University of Geneva)
Phyto- Cyano- Bloom problems (H. Paerl, University of N.Carolina)
Multi lake surveys (C. Carey, Virginia Tech)
Toxins (Miguel Lurling, University of Wageningen)
Database management (L. de Senerpont Domis, Netherlands Institute of
Ecology)
• Equipment and calibration (M. Tercier, University of Geneva)
• 2nd day:
• A, B, C teams – Field protocols, Sample handling, Microscopy
• 3nd day:
• A, B, C teams - Field protocols, Sample handling, Microscopy
• Introduction to R, Statistical Analysis (C. Carey)
Tentative programme
• European Multi lake survey – Summer 2015
• Sampling analysis in each group seperately
• Microscopy (phyto- & cyano- abundance, biomass,
biovolume)
• Chlorophyll a
• Shipment to dedicated labs:
• Toxins - LEAS (Athens)
• Flowcytometry – HOPEFULLY! Institute F.A. Forel (Geneva)
• Nutrients - ? Christoffersen University of Copenhagen…
Name
Victoria Kremser
Petar Žutinić
Maria Antoniou
Trine Warming Perlt
Kersti Kangro
Kerstin Häggqvist
Luke Brient
Hans Peter Grossart
Anastasia Hiskia
Andrea Torokne
Victor C Perello
Paola Buscarinu
Jolanta Jekabsone
Judita Koreiviene
Boris Aleksovskian
Petra Visser
Justyna Kobos
Magdalena Toporowska
Joao Morais
Zsolt Torok
Tamara Dulic
Hana Nemova
Tina Elersek
Ana Garcia Murcia
Carmen Cillero Castro
William Colom Montero
Meryem Beglioglu
Jessica Richardson
Christine Edwards
Training School Participant List
Email address
victoria.kremser@student.uibk.ac.at
petar.zutinic@gmail.com
maria.antoniou@cut.ac.cy
twperlt@bio.ku.dk
kiti@ut.ee
khaggqvi@abo.fi
luc.brient@univ-rennes1.fr
hgrossart@igb-berlin.de
hiskia@chem.demokritos.gr
torokandrea53@gmail.com
victorc.perello@dkit.ie
paola.buscarinu@enas.sardegna.it
jolanta.jekabs@gmail.com
judita.koreiviene@delfi.lt
borisaleksovskian@yahoo.com
p.m.visser@uva.nl
justyna.kobos@ug.edu.pl
magdalena.toporowska@up.lublin.pl
joaopmorais@gmail.com
zsolt.torok@ddni.ro
waterlifea@gmail.com
hana.nemova@uvzsr.sk
tina.elersek@nib.si
ana.garcia@urs.com
carmen.cillero.castro@gmail.com
william.colom@ebc.uu.se
meryem@metu.edu.tr
jerich15@ceh.ac.uk
c.edwards@rgu.ac.uk
Country
Austria
Croatia
Cyprus
Denmark
Estonia (Tartu)
Finland
France
Germany
Greece (Athens)
Hungary
Ireland (Dundalk)
Italy
Latvia (Riga)
Lithuania
Macedonia
Netherlands (Amsterdam)
Poland (Gdansk)
Poland (Lublin)
Portugal (Porto)
Romania (Tulcea)
Serbia (Novi Sad)
Slovakia
Slovenia
Spain (Barcelona)
Spain (A Coruna)
Sweden
Turkey (Ankara)
UK (Lancaster)
UK (Aberdeen)
Action
CyanoCOST
NETLAKE
CyanoCOST
CyanoCOST
NETLAKE
NETLAKE
CyanoCOST
NETLAKE
CyanoCOST
CyanoCOST
NETLAKE
CyanoCOST
NETLAKE
CyanoCOST
CyanoCOST
CyanoCOST
CyanoCOST
CyanoCOST
CyanoCOST
Neither
CyanoCOST
CyanoCOST
CyanoCOST
CyanoCOST
Neither
NETLAKE
NETLAKE
CyanoCOST
CyanoCOST
Suggestions??