TUESDAY, 23 JUNE 2015 Time MEETING ROOM A11/311

Time
09:00 -16:00
TUESDAY, 23 JUNE 2015
MEETING ROOM A11/311
MEETING ROOM A11/327
W01 – Educational Workshop
W02 – Educational Workshop
Growth and imaging techniques for laboratory
Experimental biofilm infection in animals
and clinical biofilm research
Directors: Thomas Bjarnsholt, (Denmark)
Directors: Paul Stoodley, (USA) & Claus
Sternberg, (Denmark)
Biofilm laboratory growth systems - an overview
Paul Stoodley, (The Ohio State University,
Department of Microbial Infection and Immunity,
Columbus, OH, USA)
Working with clinical and animal specimen
Thomas Bjarnsholt, (University of Copenhagen,
Faculty of Health Sciences, Department of
International Health, Immunology & Microbiology,
Copenhagen, Denmark)
Biofilm flow cells - principles and assembly
(lecture and hands on)
Paul Stoodley, (USA)
Staining biofilms for imaging - live/dead, PNA
FISH (lecture and hands on mock demo)
Kasper Nørskov Kragh, (University of Copenhagen,
Department of Systems Biology, Copenhagen,
Denmark)
Rendering confocal biofilm images (Imaris lecture and hands on)
Claus Sternberg, (Technical University of
Denmark, Department of Systems Biology Lyngby,
Denmark)
Techniques for quantifying biofilm for confocal
images (COMSTAT - lecture and hands on)
Claus Sternberg,(Denmark)
Panel Discussion
Time
16:00 - 17:15
From in vitro to in vivo – what to consider
Thomas Bjarnsholt, (University of Copenhagen,
Faculty of Health Sciences, Department of
International Health, Immunology & Microbiology,
Copenhagen, Denmark)
Animal experiments in general – legislation and
experimental designs
Claus Moser, (Rigshospitalet, Department of
Clinical Microbiology, Copenhagen, Denmark)
Evaluation of tolerance and resistance to
antibiotics in animal experiments
Oana Ciofu, (ISIM, University of Copenhagen,
Faculty of Health and Medical Sciences,
Copenhagen, Denmark)
Evaluation of inflammation and immune
response in animal experiments
Peter Østrup Jensen, (Rigshospitalet, Department
of Clinical Microbiology, Copenhagen, Denmark)
Anti-biofilm vaccine studies in animals
Mark Shirtliff, (University of Maryland,
Department of Microbial Pathogenesis, Baltimore,
MD, USA)
Transcriptomics and Tn-Seq in animal models
Kendra Rumbaugh, (Texas Tech University Health
Sciences Center - TTUHSC, School of Medicine,
Department of Surgery - The Rumbaugh Lab,
Lubbock, TX, USA) and
Marvin Whiteley, (The University of Texas,
Department of Molecular Biosciences, Austin, TX,
USA)
Lung and chronic wound models
Claus Moser, (Denmark)
Peritoneal implant and filler models
Thomas Bjarnsholt, (Denmark)
Osteomyelitis and dual infection models
Mark Shirtliff, (USA)
Participants will learn the principles behind the use
of various experimental systems to grow and image
biofilms as well as techniques to image biofilms on
clinical and ex vivo animal samples. There will be
hands on demonstrations of how to assemble a
basic flow cell system, perform PNA FISH and live
/ dead viability staining and how to best present and
quantify your confocal images using Imaris and
COMSTAT imaging software. There will be plenty
of opportunity for interactive discussion between
participants and workshop faculty during the
workshop.
AULA AUDITORIUM
Opening Ceremony
Chairs: Veronika Holá,(Czech Republic) & Filip Ruzicka, (Czech Republic)
Welcome
Veronika Holá,(EUROBIOFILMS2015 Congress President)
Welcome
Thomas Bjarnsholt, (Chairman of ESGB)
Thematic issues of P&D
Tom Coenye, (Ghent University, Laboratory of Pharmaceutical Microbiology, Ghent, Belgium)
17:15 -
Opening Lecture
The Pel polysaccharide is a positively charged exopolysaccharide that cross-links extracellular DNA
in the Pseudomonas aeruginosa biofilm matrix
Matthew R Parsek, (University of Washington, Department of Microbiology, Seattle, WA, USA)
Opening Reception
Time
09:00 - 10:00
WEDNESDAY, 24 JUNE 2015
MEETING ROOM A11/114
KL01 - Keynote Lectures
Chairs: Thomas Bjarnsholt, (Denmark) & Veronika Holá,(Czech Republic)
Insights into the function of microbial dark matters in environmental biofilms
Per Halkjær Nielsen, (Aalborg University, Department of Chemistry and Bioscience - Section for Biotech,
Aalborg, Denmark)
10:00 - 10:30
Time
10:30 - 12:30
12:30 - 13:00
13:00 - 15:00
15:00 - 15:30
Time
15:30 - 17:30
19:30 -
Title to be determined
Andrej Trampuz (Charité - University Medicine Berlin, Center for Musculoskeletal Surgery, Berlin,
Germany)
Coffee Break
MEETING ROOM A11/205
MEETING ROOM A11/114
S01 - Methods of detection & diagnosis of
S02 - Mixed species fungal & bacterial biofilm
biofilm infections
communities
Chairs: Luanne Hall-Stoodley,(USA) & Thomas
Chairs: Bastiaan Krom, (The Netherlands) &
Thomas Bjarnsholt, (Denmark)
Thomas Neu, (Germany)
Methods of detection and diagnosis of biofilm
infections
Luanne Hall-Stoodley,( The Ohio State University
College of Medicine, Department of Microbial
Infection and Immunity, Columbus, OH, USA)
Fungus-bacterium interactions in the healthy
oral cavity
Bastiaan Krom, (Academic Centre for Dentistry
Amsterdam (ACTA), Department of Preventive
Dentistry, Amsterdam, The Netherlands)
Title to be determined
Craig Williams, (University of West Scotland &
University Hospital Crosshouse, Institute of
Healthcare Associated Infection, Paisley, UK)
Lunch Break
Poster Session I
Coffee Break
MEETING ROOM A11/205
S03 - Treatment of biofilm infections
Chairs: Antonio Oliver,(Spain) & Craig Willimas,
(UK)
Lectin-barcoding of complex biofilms
Thomas Neu, (Helmholtz Center for Environmental
Research - UFZ, Department River Ecology,
Magdeburg, Germany)
Differential effect of antimicrobial drug
combinations on Pseudomonas aeruginosa and
Aspergillus fumigatus mixed microbial biofilm
Jose Vazquez,(Georgia Regents University,
Department of Infectious Diseases, Augusta, GA,
USA)
Social interactions in staphylococcal biofilms:
metabolic changes in mixed S. aureus - S.
epidermidis biofilms and the role of QS in
antibiotic tolerance
Tom Coenye, (Ghent University, Laboratory of
Pharmaceutical Microbiology, Ghent, Belgium)
MEETING ROOM A11/114
S04 - Social interactions in biofilms
Chairs: Tom Coenye, (Belgium) & Steve Diggle,
(UK)
Diagnosis and treatment of fungal biofilm
The biofilm polysaccharide PSL is a social but
infection
non-cheatable trait in Pseudomonas aeruginosa
Craig Williams, (University of West Scotland &
Steve Diggle, (University of Nottingham, School of
University Hospital Crosshouse, Institute of
Life Sciences, Nottingham, UK)
Healthcare Associated Infection, Paisley, UK)
Organ and Children Choir Concert at the Petrov Cathedral
A guided tour through the Petrov Cathedral will follow just after the concert
Time
09:00 - 10:00
THURSDAY, 25 JUNE 2015
MEETING ROOM A11/114
KL02 - Keynote Lectures
Chairs: Veronika Holá,(Czech Republic) & Thomas Bjarnsholt, (Denmark)
Biofilm slime by design: viscoelastic yielding as a virulence factor for survival in chronic infections
Paul Stoodley, (The Ohio State University, Department of Microbial Infection and Immunity, Columbus,
OH, USA)
10:00 - 10:30
Time
10:30 - 12:30
12:30 - 13:00
13:00 - 15:00
15:00 - 15:30
Time
15:30 - 17:30
Looking for new perspectives to fight bacterial biofilm infections - identification and use of biofilmspecific functions
Jean-Marc Ghigo, (Pasteur Institute, Department of Genetics of Biofilms, Paris, France)
Coffee Break
MEETING ROOM A11/132
MEETING ROOM A11/114
S05 - Host responses to biofilms
S06 - Biomechanics in biofilm and infections
Chairs: Mark Shirtliff, (USA) & Niels Høiby,
Chairs: Phil Stewart, (USA) & Henk Busscher,
(Denmark)
(The Netherlands)
The host response as an accomplice to mono and
multi-species biofilm infections
Mark Shirtliff, (University of Maryland,
Department of Microbial Pathogenesis, Baltimore,
MD, USA)
Mechanics of biofilm detachment in flowing
fluids
Phil Stewart, (Montana State University, Center for
Biofilm Engineering, Bozeman, MT, USA)
A taste for biofilms: how phagocytic cells
recognise biofilms
Gertrud M Hänsch, (University of Heidelberg,
Department of Immunology, Heidelberg, Germany)
From bacterial vibration spectroscopy on
adhering bacteria to visco-elasticity of biofilms
Henk Busscher, (The Kolff Institute at the
University Medical Center Groningen (UMCG),
Department of Biomedical Engineering, Groningen,
The Netherlands)
Lunch Break
Poster Session II
Coffee Break
MEETING ROOM A11/132
S07 - Resistance in biofilms & novel approaches
to treatment
Chairs: Gianfranco Donelli,(Italy) & Helena
Bujdakova, (Slovak Republic)
MEETING ROOM A11/114
S08 - Modelling of biofilms & regulation of
biofilm development
Chairs: David Stopar, (Slovenia) & Kendra
Rumbaugh, (USA)
Rationally designing nanomedicines for the
eradication of pulmonary biofilm infections
Katrien Forier,( Ghent University, Department of
Clinical Chemistry, Microbiology and Immunology,
Ghent, Belgium)
Gradual transition into a floating biofilm
David Stopar, (University of Ljubljana, Department
of Food Science and Technology, Ljubljana,
Slovenia)
Photodynamic inactivation - a possible
alternative in eradication of resistant microbial
biofilms
Helena Bujdakova, (Comenius University In
Bratislava, Faculty of Natural Sciences
Department of Microbiology and Virology,
Bratislava, Slovak Republic)
Modeling polymicrobial wound infections
Kendra Rumbaugh, (Texas Tech University Health
Sciences Center - TTUHSC, School of Medicine,
Department of Surgery - The Rumbaugh Lab,
Lubbock, TX, USA)
Time
09:00 - 11:00
FRIDAY, 26 JUNE 2015
MEETING ROOM A11/132
MEETING ROOM A11/114
S09 - Probiotics and new natural & synthetic
S10 - Horizontal gene transfer, evolution &
anti-biofilm compounds
heterogeneity in microbial biofilms
Chairs: Christine Imbert, (France) & Claudia
Chairs: Alex O'Neill, (UK) & Mette Burmølle,
Vuotto, (Italy)
(Denmark)
Probiotics and new natural and synthetic antibiofilm compounds
Elizabeth Presterl, (Medical University of Vienna,
Department of Internal Medicine I, Division of
Infectious Diseases and Tropical Medicine, Vienna,
Austria)
11:00 - 11:30
Time
11:30 - 13:30
Accelerated evolution of antibiotic resistance in
staphylococcal biofilms
Alex O'Neill, (University of Leeds, School of
Molecular and Cellular Biology (SMCB), Leeds,
UK)
Probiotics: a potential tool to counteract biofilm- Do plasmids drive their host into the biofilm
based infections
lifestyle and why would they?
Claudia Vuotto, (IRCCS Fondazione Santa Lucia,
Mette Burmølle, (University of Copenhagen,
Microbial Biofilm Laboratory, Rome, Italy)
Department of Biology, Copenhagen, Denmark)
Coffee Break
MEETING ROOM A11/132
S11 - Environmental biofilms and human health
Chairs: Andrew McBain, (UK) & Martin Rulik, (Czech Republic)
Microbicide-exposure and antimicrobial resistance in biofilms in the domestic environment
Andrew McBain, (University of Manchester, Manchester Pharmacy School, Manchester, UK)
Bacterial - Trojan Horse interactions: the breakthrough of the new approaches to their study
Katarina Trnková, (University of Matej Bel, Faculty of Natural Sciences, Department of The Environment,
Banská Bystrica, Slovak Republic)
13:30 -
Microfluidic high-throughput platform for biofilm analysis
Tereza Hájková, (BioTech Europe, Prague, Czech Republic)
Closing Ceremony
Poster Awards