Thomas Raymond Unnasch - USF Health

Transcription

Thomas Raymond Unnasch
Distinguished USF Health Professor and State of Florida World Class Scholar
Global Health Infectious Disease Research Program
Department of Global Health
University of South Florida
3720 Spectrum Blvd., Suite 304
Tampa, FL 33612
Home: (813) 345-8336 Work: (813) 974-0507/7807 Fax: (813) 974-0992
E-mail: tunnasch@health.usf.edu
Education
1976- Massachusetts Institute of Technology: PhD under the direction of
1981
Dr. Gene M. Brown. Thesis entitled “The Purification and Properties
of Dihydropterin Oxidase from Drosophila melanogaster.”
1972- Rutgers University: A.B. with highest honors
1976
Major in microbiology. Thesis entitled “The Effect of Cholera
Enterotoxin on the Adenyl Cyclase of Escherichia coli.”
Employment
2012 present
Distinguished USF Health University Professor, State of Florida
World Class Scholar and Chair, Department of Global Health,
University of South Florida College of Public Health
20082012
University of South Florida: Professor and State of Florida World
Class Scholar, Global Health Infectious Disease Research, Department of
Global Health. Joint Appointment in Molecular Medicine
1997- University of Alabama, Birmingham: Professor, Division
2007
of Geographic Medicine, Department of Medicine, with joint
appointments in the Departments of Microbiology and Epidemiology
and International Health.
1
1989 1997
University of Alabama, Birmingham: Associate Professor, Division
of Geographic Medicine, Department of Medicine
1985- Case Western Reserve University: Assistant Professor, Division
1989
of Geographic Medicine, Department of Medicine
19821985
Harvard School of Public Health: Postdoctoral fellow in the
Department of Tropical Public Health (laboratory of Dr. Dyann F. Wirth)
Peer Review Panels and Editorial Boards
1986- Temporary member, Scientific Working Group, Onchocerciasis
1993
Chemotherapy Project, World Health Organization
1988- Editorial Board, Experimental Parasitology
2002
1990- Ad Hoc Reviewer, Filariasis Scientific Working Group, World
Present
Health Organization
1990- Ad Hoc Member, Bacteriology and Mycology Study Section, NIAID
1992
1993
Member, Review Committee, “Basic and Applied Research into
Tropical Diseases”, NIAID
1993
Member, Source Selection Committee, “Basic and Applied Research
into Tropical Diseases”, NIAID
1993- Member, TMP/AHR Study Section
2002
1995
Chairman, Site Visit Committee AID-NIAID Middle Eastern 1996 Research
Cooperative (MERC) Program
2003- Editorial Board, Molecular and Biochemical Parasitology
present
2
2004- Chair, Assay and Methods Development Study Section, National 2006
Institutes of Health
2005- Scientific Advisory Board, WIRM Initiative, Scripps Institute, La Jolla, 2006
CA
2006
Ad Hoc reviewer, Pathogenic Eucaryotes Study Section, National
Institutes of Health
2006
Reviewer, Pathways Program, National Institutes of Health
2006
Member, Drug Design Research Steering Committee, Special
Programme for Research and Training in Tropical Diseases, World
Health Organization
2007
External Reviewer, SBIR Program, United States Department of
Agriculture
2007- Editorial Board Advisor, PloS Neglected Tropical Diseases
present
2007- Member, Ecology of Infectious Diseases Proposal Review Panel, 2008
National Science Foundation
2009
Member “Topics in Microbiology” Study Section, NIAID
2009- Editorial Board, Journal of Tropical Medicine
2012
2010- Editor in Chief, Research Reports in Tropical Medicine
present
20102012
Member Working Group on Eukaryotic Pathogens and Disease
Vectors Target Selection, pathogens genomic sequencing initiative,
National Institutes of Health
2014
Chair, NIH BSTF55 Indo-US Collaboration Grant Study Section
2015
Chair, NIH PAR14-092 Bioengineering Research Partnership Study
Section
Manuscript Reviewer for
Acta Tropica
American Journal of Tropical Medicine and Hygiene
Annals of Epidemiology
Biochimica et Biophysica Acta
3
European Journal of Biochemistry
Experimental Parasitology
FASEB Journal
Filarial Journal
Genomics
Infection and Immunity
Insect Molecular Biology
International Journal for Parasitology
Journal of Biological Chemistry
Journal of Medical Entomology
Journal of Immunology
Journal of Infectious Diseases
Journal of Parasitology
The Lancet
Microbial Reviews
Memórias do Instituto Oswaldo Cruz
Molecular and Biochemical Parasitology
Molecular Phylogenetics and Evolution
Nature Protocols
Parasitology
Parasitology Today
PLOS ONE
PLOS Neglected Tropical Diseases
Transactions of the Royal Society of Tropical Medicine and Hygiene
Trends in Parasitology
Tropical Medicine and International Health
Invited Presentations
1986
1986
1987
1988
1988
1989
1989
1989
1989
1989
Invited speaker, University of Edinburgh, Edinburgh, Scotland
Invited speaker, Rockefeller Great Neglected Diseases Program
Meeting, Woods Hole Massachusetts
Invited speaker, McArthur Foundation Symposium on Parasitic
Diseases, Pleasant Town, New York
Invited speaker, Texas College of Osteopathic Medicine, Fort Worth
Texas
Invited speaker, Division of Infectious Diseases, University of
Alabama at Birmingham, Birmingham, Alabama
Invited speaker, O Now! Symposium on Onchocerciasis, University of
Leiden, Leiden, the Netherlands
Invited speaker, Bernhard Nocht Institute for Tropical Medicine,
Hamburg, Germany
Invited Speaker, University of Heidelberg, Heidelberg, Germany
Invited participant, WHO workshop “ DNA probes for Filariasis and
Onchocerciasis”, Jakarta, Indonesia
Invited speaker, 24th Joint Conference on Parasitic Diseases, The
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1991
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1991
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1993
1993
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1997
1998
US-Japan Cooperative Medical Science Program of the National
Institutes of Health, Madison Wisconsin
Invited speaker, Symposium: Nucleic Acid Based Diagnostics:
Applications of the Polymerase Chain Reaction” 39th Annual
Meeting of the American Society of Tropical Medicine and Hygiene,
New Orleans, Louisiana
External participant, Internal Technical Review Meeting of the
Onchocerciasis Control Programme in West Africa, Ouagadougou,
Burkina Faso
Invited speaker, Agouron Institute, San Diego, California
Invited participant, WHO workshop, “DNA probes for Diagnosis of
Human and Animal Onchocerca” Bouake, Cote d’Ivoire
External participant, Internal Technical Review Meeting of the
Onchocerciasis Control Programme in West Africa, Ouagadougou,
Burkina Faso
Lecturer, MBL Summer Course “Biology of Parasitism” Woods Hole,
Massachusetts
Invited speaker, 2nd annual ICTDR meeting of the National
Institutes of Health, Bethesda, Maryland
Invited participant, WHO workshop “DNA Probes and PCR for
Detection of Filarial Parasites in Vectors” Beverly, Massachusetts
Institutes of Health, Baltimore, Maryland
Lecturer, MBL Summer Course “Biology of Parasitism” Woods Hole,
Massachusetts
Invited Speaker and Rapporteur, WHO workshop “The Filarial
Genome Project” Beverly Massachusetts
Invited speaker, “Immunogenetics and Epidemiology of Aveolar
Echinococcosis”, Schloss Reisenburg, Gunzburg, Germany
Invited speaker, Fourth International Conference on Onchocerciasis,
Pan American Health Organization, Washington, D.C.
Invited Speaker, Institut Pierre Richet, OSTROM, Bouake Côte
d’Iviore
Institutes of Health, Cleveland, OH.
Invited Speaker, Centers for Disease Control, Atlanta, GA.
Invited Speaker, International Workshop on Molecular Epidemiology
and Evolutionary Genetics of Pathogenic Microorganisms, Centers
for Disease Control and Prevention and ORSTOM, Atlanta, GA.
Invited speaker and rapporteur, O. volvulus Diagnostic methods
workshop, World Health Organization, Geneva
Keynote speaker, American Society of Tropical Medicine and
Hygiene, 59th Annual Meeting
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1998
2000
2000
2002
2002
2003
2004
2004
2004
2004
2005
2005
2005
2006
2006
2006
2006
2006
2007
2007
2008
2008
2008
2008
2009
2009
2009
Invited speaker, Workshop on macrofilaricidal targets, World Health
Organization, Geneva
Noguchi Memorial Institute for Medical Research, Legon, Ghana
Invited speaker, Black Flies in the New Millennium, Brock University,
St. Catherine’s, Ontario, Canada
Invited speaker, University of Georgia, Athens, GA.
Invited speaker, McGill University, Montreal Canada
Invited Speaker, Filarial Research Summit, Baltimore Md.
Invited participant, Brugia malayi genome annotation jamboree, The
Institute for Genome Research, Rockville, MD.
Invited Speaker, University of South Alabama, Mobile, AL
Participant, Global 2000 Onchocerciasis Annual Review, Carter
Center, Atlanta, GA.
Presenter, IACO meeting, Carter Center, Atlanta, GA
Invited speaker, Annual Meeting of the American Thoracic Society, San
Diego, CA
Invited speaker, Centro De Biotecnología Genómica, Instituto Politécnico
Nacional, Reynosa, Mexico
Symposium speaker, 8th National Conference on West Nile virus,
American Society of Microbiology and Centers for Disease Control and
Prevention, San Francisco, CA.
Participant and Presenter, 10th International Congress on Onchocerciasis
in the Americas, Antigua, Guatemala
Participant, Eukaryotic Pathogens and Vectors Sequencing Workshop,
National Human Genome Research Institute
Seminar Speaker, University of Pennsylvania
in the Americas, Quito, Ecuador
Seminar Speaker, New England Biolabs, Ipswitch, MA
Scientific Advisory Board, WHO Multi-Disease Research Centre,
Ouagadougou, Burkina Faso
in the Americas, Antigua, Guatemala
Seminar Speaker, University of Georgia
in the Americas, Oaxaca, Mexico
6
2010
2011
2011
2012
2012
in the Americas, Quito, Ecuador
in the Americas, Bogota, Colombia
Seminar Speaker, Case Western Reserve University
Educational Activities
Didactic Teaching:
Prior to USF:
1978 &
1980
Teaching assistant, Biochemistry (course 7.05),
Massachusetts Institute of Technology
1981
Director, Biology teaching laboratory (course 7.011)
Massachusetts Institute of Technology
1990 &
1992
Course Director, Biology of Parasitism, Department of
Microbiology, University of Alabama at Birmingham
1994
Lecturer, Biology of Parasitism, Woods Hole, MA
1995- Lecturer, Tropical Infectious Diseases, Department of
2007
Epidemiology and Public Health, University of Alabama
at Birmingham (Arboviruses and onchocerciasis)
2003- Lecturer, Medical Microbiology, University of Alabama
2007
at Birmingham School of Medicine (Emerging viruses
and helminthic Infections).
1995- US mentor, NIH Minority International Research Training present
Program, University of Alabama at Birmingham
2003- Member, Department of Medicine Education Committee
2007
University of Alabama at Birmingham
2006- Principal Investigator, Bioterrorism and Emerging
2007
Infectious Diseases Training Grant, University of
Alabama at Birmingham
At USF:
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Course Director:
2009
PHC 6907 Independent Study
PHC 6945 Supervised Field Experience
PHC 6971 Thesis
PHC 7910 Directed Research
2010
PHC 6907 Independent Study
PHC 6934-901 Laboratory Techniques in Public Health
PHC 6971 Thesis
PHC 7908 Specialized Study
2011
PHC 6934-901 Laboratory Techniques in Public Health
PHC 6971 Thesis
PHC 6977 Special Project
2012
PHC 6934 Tools of Research: Laboratory Rotations
PHC 7931-001 Advanced Interdisciplinary Seminar: Current topics
in Global Health Infectious Disease Research
PHC 7980 Dissertation
2013
PHC 6934 Tools of Research: Laboratory Rotations
PHC 7931-001 Advanced Interdisciplinary Seminar: Current topics
in Global Health Infectious Disease Research
Guest lecturer for:
2009:
PHC 6512 Vectors of human Disease
2010:
PHC 6510 Emerging and Infectious Diseases
BMS 6041 Medical Sciences 5
2011:
PHC 6510 Emerging and Infectious Diseases
GMS 6103 Foundations in Medical Microbiology and Immunology
2012
GMS 6103 Foundations in Medical Microbiology and Immunology
8
PhD level and post-graduate laboratory based training
Pre-Doctoral/Postdoctoral Trainees in Thomas R. Unnasch Laboratory
1. Klaus Erttmann, Postdoctoral fellow, 1986-1989
Strain and Species Specific DNA probes for O. volvulus
Present position: Bernhardt Nocht Institute, Hamburg, Germany
2. Michael Kron, Postdoctoral fellow, 1987-1989
Analysis of structure of an aspartyl tRNA synthetase gene from O. volvulus
Present position: Professor, Medical College of Wisconsin
3. Peter Zimmerman, Graduate Student 1989-2003 (Ph.D granted 2003)
Evolution of a repeated sequence family in O. volvulus
Present position: Professor, Case Western University
4. James Tang, PhD, Postdoctoral fellow, 1993-1996
Vector-parasite transmission complexes in human onchocerciasis
Present Position: Professor, Division of Infectious Diseases, University of Alabama at
Birmingham
5. Elise Keddie, Graduate Student (Ph.D granted 1997)
Mitochondrial structure in Onchocerca volvulus
Present Position: Manager, Zero Emission Vehicle Implementation, California Air
Resources Board
6. Nancy Maher, Graduate Student (Ph.D granted 2000)
Molecular Epidemiology of Pneumocystis carinii
Present position: Program Manager, VA non-profit office, Veterans Health
Administration
7. Daniel Boakye, Postdoctoral Fellow, 1996-1999
Microsatellites to detect hybridization between O. volvulus strains
Present position: Head, Entomology Unit, African Programme for Onchocerciasis
Control
8. Joon Hak Lee, Ph.D Postdoctoral fellow, 1998-2000
Ecology of Encephalomyelitis Virus Infection in the Southeastern USA
Present Position: Staff Scientist, Sullivan County Department of Health, New York
9. Tarig Higazi, Ph.D, Postdoctoral fellow, 1998-2006
Promoter Analysis of B. malayi
Present Position: Associate Professor, Ohio University, Zanesville
9
10. Limin Shu, Ph.D, Postdoctoral fellow, 2001-2004
Tansfection and Immortalization of filarial nematodes
Present Position: Research Associate Professor, Rutgers University
11. Sunny Yung, BS, MD-Ph.D Student (Ph.D granted 2002)
Plastid targeting in Toxoplasma gondii
Present position: Research fellow, National Institutes of Health
12. Tonya Korves, BS, MS MPH student 2003-2005 (MPH granted 2005)
Ecology of Eastern encephalomyelitis Virus in the Southeastern USA
Present position: Professor, St. Louis Community College at Wildwood
13. Gregory White, Graduate Student 2006-2011(Ph.D granted 2011)
Ecology of Eastern Equine Encephalitis Virus in the Southeastern USA
Present position: Scientific Director, Cochilla Valley Mosquito Control District.
14. Christy Ottendorfer, Ph.D postdoctoral fellow 2008-2010
Ecology of Encephalitis Viruses in Florida
Present Position: Microbiologist, Centers for Disease Control and Prevention, Atlanta,
GA
15. Chitra Chauhan, Ph.D postdoctoral fellow, 2008-2010 (deceased)
Transfection and analysis of transcription in Brugia malayi
16. Shulin Xu, DVM, postdoctoral fellow 2008-2011
Mapping Protein Interactions between Filaria and its Wolbachia Endosymbiont
Present position: Post-doctoral fellow, University of South Florida
17. Canhui Liu, Ph.D postdoctoral fellow 2006-present
Transfection and analysis of transcription in Brugia malayi
18. Nathan Burkett-Cadena, Ph.D Postdoctoral Fellow, 2010-2013
Development of an improved black trap for onchocerciasis monitoring and control.
Present position: Assistant Professor, Department of Entomology, University of Florida
19. Patrick Vander Kelen, Ph.D Student 2009-2012
Spatial modeling of Eastern Equine Encephalitis Virus foci in Florida. Present Position:
Staff Scientist, Oregon Department of Health
20. Andrea Bingham, Ph.D Student, 2010-2014
Mechanisms of over-wintering and early season amplification of Eastern Equine
Encephalitis Virus. Present position: Vector-Borne Disease Surveillance Coordinator,
Florida Department of Health
21. Amruta Mashlikar, PhD Student 2012-present
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Ecdysteroid signaling in human filarial parasites
Masters of Science in Public Health Students in Thomas R. Unnasch Laboratory
1. Jessie Dyer 2008-2010. MSPH granted 2010. Thesis entitled “Characterization of
unknown viruses from Florida”
2. Michelle Bailey 2009-2010. MSPH granted 2010. Thesis entitled “Core Promoter
Function in B. malayi”
3. Andrea Bingham 2009-2011. MSPH granted 2011. Thesis entitled “Development of
an ELISA for Eastern Equine Encephalitis Virus that can Differentiate Infected from
Vaccinated Horses”
4. Tracy Enright 2009-2011. MSPH granted 2011. Thesis entitled “Analysis of Ecdysone
response elements in the genome of Brugia malayi”
5. Alex Talsma 2011-2013 MSPH granted 2013. Thesis entitled “Development of a
confirmatory assay to detect Onchocerca volvulus larvae in pools of blackflies”
6. Shanna Boclen 2012-2014 MSPH granted 2014. Thesis entitled “Molecular
Evidence for Vector Implication of Onchocerca lupi in Los Angeles County, CA”
7. Monica Lanzaro 2013-2015 MSOH granted 2015.Thesis entitled “Gene Expression
Analysis Of Upregulated Genes By 20-OH Ecdysone in Brugia malayi
Short-term trainees trained in USA from the following countries:
Brazil
Burkina Faso
Cameroon
Canada
Colombia
Cote d’Ivoire
Ecuador
Ethiopia
Guatemala
Ghana
Mexico
Nigeria
Venezuela
Sudan
Uganda
Supervision of Overseas Research Studies by USA Students in:
Burkina Faso
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Cote d’Ivoire
Ghana
Guatemala
Uganda
Administrative Experience:
2012 – present
Chair, Department of Global Health, USF
2009 - 2012 Co-Director, Center for Biological Defense, USF
2008 – present
Director and Principal Investigator, BSL3 facility, IDRB
University Service:
2011 – 2012 COPH Research Advisory Committee
2012
COPH Appointment, promotion and tenure (APT)
committee
2010 – 2012 Global Health APT committee
2012
Chair, Global Health APT committee
2012
Chair, Global Health PhD application review committee
External/International Service:
1993- Member, Program Committee, American Society of Tropical
1996
Medicine and Hygiene
1987- Member, Young Investigator Award Committee, American Society of
1993
Tropical Medicine and Hygiene
19922000
Temporary Technical Advisor, Onchocerciasis Control Programme in
West Africa, Ouagadougou, Burkina Faso
2001- Technical Advisor, Onchocerciasis Elimination Program in the
present
Americas, Guatemala City, Guatemala
2001present
Technical Advisor, Molecular Monitoring, River Blindness Program,
The Carter Center, Atlanta, GA
2008- Technical Advisor, African Programme for Onchocerciasis Control,
present
Ouagadougou, Burkina Faso
2008- Member, Ugandan Onchocerciasis Elimination Committee, Ugandan 2011
Ministry of Health
2011
present
Chair, Ugandan Onchocerciasis Elimination Expert Advisory
Committee, Uganda Ministry of Health
2011
Member, Board of Directors, Florida Mosquito Control Association
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present
2012 present
Member, Mectizan Expert Committee, Mecitzan Donation Program,
Atlanta, GA
2012 present
Member, Guidelines Development Group, World Health Organization
Geneva
2013 - Member, Technical Coordinating Committee (TCC) African
2015
Programme for Onchocerciasis Control
2014- Chair, Transition Task Force, (TTF) African Programme for
2015
Onchocerciasis Control
2014- Member, Ethiopian Onchocerciasis Elimination Expert Advisory
present
Committee, Ethiopian Ministry of Health
Honors and Awards:
1975
1976
1982
1982
2007
2010
2012
2014
Henry Rutgers Scholar
Recipient, Theobold Smith Society Award
Recipient, individual NRSA fellowship
Alternate, Helen Hay Whitney Fellowship
State of Florida World Class Scholar
Outstanding Research Achievement Award, University of South Florida
Distinguished USF Health Professor, University of South Florida
Fellow, American Society of Tropical Medicine and Hygiene
Funding History
Past Grant Support:
1. WHO # 860275 “Development of a DNA Based Assay to Distinguish Forest and
Savannah Strains of O. volvulus. 1/1/87-6/30/90 $110,036.
2. WHO # 870068 “Characterization of a Gene for a Potentially Protective Antigen of
Onchocerca volvulus.” 7/1/87-6/30/89 $73,750.
3. Edna McConnell Clark Foundation # 05189 “Characterization and Molecular Cloning
of Potentially Protective Antigens of O. volvulus” (Listed as Co-P.I.) 3/1/89-2/28/91
$198,000.
4. WHO # 890307 “Refinement of a DNA Probe Based Assay to Distinguish Forest
and Savannah Forms of Onchocerca volvulus.” 7/1/90-12/31/92 $110,013.
5. Edna McConnell Clark Foundation “Potentially Protective Peptide Antigens of O.
volvulus” 4/1/90-9/30/93 $390,000.
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6. NIH Program Project “Molecular Parasitology and Parasitic Disease.” David O.
Freedman, Program Director. P.I. on Sub-Project titled: “Molecular Cloning of Disease
Associated Antigens” 3/1/91-12/31/95 $383,780.
7. NIH 1R29AI29693 “Identification of O. volvulus Protective Epitopes.” 8/1/89-7/31/95
$349,995.
8. WHO #920288 “Analysis of a Repeated Sequence Family to Study the Relationship
Between Onchocerca volvulus Strains” 1/1/93-6/30/96 $71,196.
9. Edna McConnell Clark Foundation “Steroid Receptors as Vaccine Candidates
Against Onchocerca volvulus. 4/1/93-3/31/96 $225,000.
10. NIH 1RO1AI33008 “Vector-Parasite Complexes in Onchocerciasis” (TR Unnasch,
PI) 5/1/93-4/30/97 $327,539.
11. WHO #950453 “Strain Variation in Pathogenetic Autoantigen Genes in Onchocerca
volvulus. (TR Unnasch, PI) 1/1/96-12/31/97 $57,574.
12. Edna McConnell Clark Foundation “Molting regulatory and biochemical activities as
vaccine targets against Onchocerca volvulus. 4/1/96-12/31/97 (TR Unnasch, PI)
$80,000
13. NIH R01 AI 35938 “Cytokine based therapy of Onchocerca antigen induced keratitis
in the mouse” (E. Pearlman, PI, TR Unnasch, co-investigator) 4/1/94-3/31/98 $67,205
(TR Unnasch’s subproject)
14. WHO 960040 “Prolyl 4-hydroxylase as a Chemotherapeutic Target against
Onchocerca volvulus” (TR Unnasch, PI) 8/1/96-7/31/98 $134,988
15. NIH 1 R21 AI39245-01 “Molecular Epidemiology of Onchocerciasis in Nigeria”
9/23/96-9/22/98 (TR Unnasch, PI) $116,302.
16. Edna McConnell Clark Foundation “Use of Pantropic retroviruses to develop an
immortalized cell line from Onchocerca volvulus.” (TR Unnasch, PI) 01/01/98 - 12/31/99
$119,000
17. WHO #970476 “Microsatellite Loci to Detect Hybridization between Onchocerca
volvulus strains” (TR Unnasch PI) 11/1/98 - 01/06/01 $38,541
18. WHO “Characterization of Angiogenic Proteins from Onchocerca volvulus.” (TR
Unnasch PI) 01/01/00 = 12/31/01 $153,513
19. WHO #991105 “Survey of genetic polymorphisms in Loa loa associated with
adverse reactions in ivermectin.” (TR Unnasch PI) 01/01/01 - 12/31/01 $ 12,338
20. Onchocerciasis Control Programme “Research of DNA Probes for Onchocerca spp.
and Validation of Species Identification Performed by the OCP. (TR Unnasch PI) 1/1/9312/31/02 $235,000.
21. NIAID 1 R01 AI54586 “Environmental and Molecular Epidemiology of P. carinii” (TR
Unnasch Co-PI) 8/15/98 - 4/30/03 $165,530
22. Case Western Reserve University\WHO “Role of chemokines in the Pathogenesis of
Onchocercal Dermatitis” (TR Unnasch PI) 01/01/99 - 12/31/03 $8,505
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23. Tennessee Valley Authority #98RE4-233022 “Pool screen PCR for detecting
encephylamyelitis viruses in mosquitoes in the Tennessee River Valley Authority area.”
1/01/00 - 12/01/04 $325,000
24. Case Western Reserve University\WHO “Role of chemokines in the Pathogenesis of
Onchocercal Dermatitis” (E. Pearlman, PI) 01/01/05 - 12/31/05 $7,500
25. NIAID 1R01TW/ES01560 Ecology of Encephalitis virus in the Southeastern USA
10/1/00-6/30/05 $1,350,868
26. NIAID/NIH 5 T32 AI055438 Agents of International Health and Bioterrorism Concern
09/01/2003-12/31/2007 $1,234,050
27. NIAID 1R01CI00226 Ecology of West Nile Virus in the Southeastern USA 9/27/043/31/08 $525,000
28. NIAID 2R01AI48562 “Transfection and Analysis of Transcription in B. malayi”
1/1/01-1/31/10 $1,825,000
29. Department of Defense (FBRC through USF CBD) “Development of Rapid Methods
for Improving PH Lab Detection of Emerging Arboviruses” 8/4/10 – 8/31/12 $ 107,480.
30. Carter Center/Global 2000 Program “PCR pool screening to monitor progress
towards onchocerciasis elimination” 1/1/01-8/31/12 $242,000
31. NIH/NIAID 2 R01 AI049724-05 “Ecology of Encephalitis Viruses in the USA” 3/1/062/28/13 $2,263,765
32. NIH/NIAID R01AI072465-01A1”Mapping Protein Interactions between Filaria and
its Wolbachia Endosymbiont” 5/1/08 - 4/30/13 $1,608,389.
33. Department of Defense (FBRC through USF CBD) “Ligands Expressing
Bacteriophage as Anti-Viral Agents” 8/17/11 – 7/24/13 $76,468.
Active Grant Support:
1. NIH Fogerty International Center #R01TW008508 “Spatial modeling of
onchocerciasis foci in Africa by remote sensing” 08/15/09 – 11/30/15 $1,572,200.
2. Bill & Melinda Gates Foundation #OPP1017870 “Development of an improved black
fly trap for onchocerciasis monitoring and control” 09/30/10 – 12/31/15 $2,142,862
3. NIH/NIAID R56AI01372-01A1 “Molecular Mechanisms of Filarial Endosymbiosis”
(co-PI) 06/15/13 – 05/31/15 $124,374
4. NIH/NIAID 1R21AI103302-01 “Ecdysteroid Signaling in Filarial Parasites” 08/15/13 –
07/31/15 $217,647
5. NIH/NIAID 1R56AI101072-01 “Delineating EEEV Over-Wintering and Early Season
Amplification Mechanisms” 08/01/13 – 07/31/15 $343,255
6. The Carter Center, Inc. “Onchocerca volvulus Parasite Detection” 09/01/13 –
08/31/15 $139,876
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Publications
Peer Reviewed Research Articles:
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Burkett-Cadena, N.D., A.M. Bingham, B. Hunt, G. Morse, and T.R. Unnasch, Ecology of Culiseta
melanura and Other Mosquitoes (Diptera: Culicidae) from Walton County, Florida During Winter
Period 2013-2014. Journal of Medical Entomology, 2015. in press:
Hassan, H.K., S. Bolcen, J. Kubofcik, T.B. Nutman, M.L. Eberhard, K. Middleton, J.W. Wekesa,
G. Ruedas, K.J. Nelson, R. Dubielzig, M. De Lombaert, B. Silverman, J.J. Schorling, P.H. Adler,
T.R. Unnasch, and E.S. Beeler, Isolation of Onchocerca lupi in dogs and black flies, California,
USA. Emerging Infectious Diseases, 2015. 21:789-796.
Liu, C., D. Voronin, C.B. Poole, S. Bachu, M.B. Rogers, J. Jin, E. Ghedin, S. Lustigman, L.A.
McReynolds, and T.R. Unnasch, Functional Analysis of MicroRNA activity in Brugia malayi.
International Journal for Parasitology, 2015. in press:
McGaha, T.W., R.M. Young, N. Burkett-Cadena, J.P. Iburg, J.M. Beau, S. Hassan, C.R. Katholi,
E.W. Cupp, B.J. Baker, T.R. Unnasch, and R. Noblet, Identification Of Communal Oviposition
Pheromones From The Black Fly Simulium vittatum. PLOS One, 2015. 10:e118904.
Young, R.M., N.D. Burkett-Cadena, T.W. McGaha, M.A. Rodriguez-Perez, L.D. Toé, M.A.
Adeleke, M. Sanfo, T. Soungalo, C.R. Katholi, R. Noblet, H. Fadamiro, J.L. Torres-Estrada, M.C.
Salinas-Carmona, B. Baker, T.R. Unnasch, and E.W. Cupp, Identification of Human
Semiochemicals Attractive to the Major Vectors of Onchocerciasis. PLOS Neglected Tropical
Diseases, 2015. 9:e3450.
Bingham, A., N. Burkett-Cadena, H.K. Hassan, C.J.W. McClure, and T.R. Unnasch, Field
investigations of winter transmission of Eastern Equine Encephalitis virus in Florida. American
Journal of Tropical Medicine and Hygiene, 2014. in press:
Burkett-Cadena, N., A. Bingham, C. Porterfield, and T.R. Unnasch, Innate preference or
opportunism: Mosquitoes feeding on birds of prey at the Southeastern Raptor Center. Journal of
Vector Ecology, 2014. 39:21-31.
Burkett-Cadena, N. and T.R. Unnasch, Conspiring invasive species? The case of Tiger
Mosquitoes and Apple Snails. Wingbeats, 2014. Spring:4-9.
Burkett-Cadena, N.D., A.M. Bingham, and T.R. Unnasch, Sex-based avian host use by arbovirus
vectors. Royal Society Open Science, 2014. 1:140262.
Jacob, B., R.J. Novak , L. Toe, M.S. Sanfo, S. Caliskhan, A. Pare, M. Noma, L. Yameogo, and T.
Unnasch, Unbiasing a Stochastic Endmember Interpolator Using ENVI Object-Based Classifiers
and Boolean Statistics for Forecasting Canopied Simulium damnosum s.L. Larval Habitats in
Burkina Faso. Geophysics and Remote Sensing, 2014. in press:
Jacob, B.G., R.J. Novak, L. Toe, M.S. Sanfo, R. Tibgueria, A. Pare, M. Noma, D. Griffith, and
T.R. Unnasch, Denoising a box-jenkins autoregressive-integrated moving average model
employing automated bandwidth selection procedures and pre-whitened Euclidean-based
quadratic surrogates in PROC ARIMA for optimizing asymptotic expansions and simulations of
onchocerciasis endemic transmission zones in Burkina Faso. Journal of Public Health and
Epidemiology, 2014. in pres:
Jacob , B.G., R.J. Novak , L.D. Toe, M.S. Sanfo, S. Caliskan, J. Hasemann, M. Noma, L.
Yameogo, and T.R. Unnasch, Deterministic variographic error optimization techniques in ArcGIS
Geostatistical Analyst® employing surrogate probabilistic Gaussian-based metamodels for
forecasting Similium damnosum s.l. riverine larval habitats Trends in GIS, 2014. in press:
Katabarwa, M., T. Lakwo, P. Habomugisha, S. Agunyo, E. Byamukama, D. Oguttu, R.
Ndyomugyenyi, E. Tukesiga, G.O. Ochieng, F. Abwaimo, A. Onapa, D.W. Lwamafa, F. Walsh,
T.R. Unnasch, and F.O. Richards, Transmission of Onchocerca volvulus by Simulium neavei in
Mount Elgon focus of Eastern Uganda has been interrupted. American Journal of Tropical
Medicine and Hygiene, 2014. 90:1159-66.
Lovato, R., A. Guevara, R. Guderian, R. Proano, T. Unnasch, H. Criollo, H.K. Hassan, and C.D.
MacKenzie, Interruption of Infection Transmission in the Onchocerciasis Focus of Ecuador
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Leading to the Cessation of Ivermectin Distribution. PLOS Neglected Tropical Diseases, 2014. 8:
e2821.
Lustigman, S., E. Melnikow, S.B. Anand, A. Contreras, V. Nandi, J. Liu, A. Bell, T.R. Unnasch,
M.B. Rogers, and E. Ghedin, Potential involvement of Brugia malayi cysteine proteases in the
maintenance of the endosymbiotic relationship with Wolbachia. International Journal for
Parasitology: Drugs and Drug Resistance, 2014. 4:267-277.
Oguttu, D., E. Byamukama, C.R. Katholi, P. Habomugisha, C. Nahabwe, M. Ngabirano, H.K.
Hassan, T. Lakwo, M. Katabarwa, F.O. Richards, and T.R. Unnasch, Serosurveillance to Monitor
Onchocerciasis Elimination: The Ugandan Experience. American Journal of Tropical Medicine
and Hygiene, 2014. 90:339-345.
Rodríguez-Pérez, M.A., M.A. Adeleke, I.C. Rodríguez-Luna, E.W. Cupp, and T.R. Unnasch,
Evaluation of a community-based trapping program to collect Simulium ochraceum sensu lato for
verification of onchocerciasis elimination. PLOS Neglected Tropical Diseases, 2014. 8:e3249.
Toé, L.D., L. Koala, N.D. Burkett-Cadena, B.M. Traoré, M. Sanfo, S.R. Kambiré, E.W. Cupp, S.
Traoré, L. Yameogo, D. Boakye, M.A. Rodríguez-Pérez, and T.R. Unnasch, Optimization of the
Esperanza Window Trap for the collection of the African onchocerciasis vector Simulium
damnosum sensu lato. Acta Tropica, 2014. 137:39-43.
Vander Kelen, P., J.A. Downs, T. Unnasch, and L. Stark, A risk index model for predicting eastern
equine encephalitis virus transmission to horses in Florida. Applied Geography, 2014. 48:79-86.
Estep, L.K., C.J. McClure, P. Vander Kelen, N.D. Burkett-Cadena, S. Sickerman, J. Hernandez,
J. Jinright, B. Hunt, J. Lusk, V. Hoover, K. Armstrong, L.M. Stark, G.E. Hill, and T.R. Unnasch,
Risk of exposure to eastern equine encephalomyelitis virus increases with the density of northern
cardinals. PLOS One, 2013. 8:e57879.
Golden, A., C. Steel, L. Yokobe, E. Jackson, R. Barney, J. Kubofcik, R. Peck, T.R. Unnasch, T.B.
Nutman, T. de Los Santos, and G.J. Domingo, Extended Result Reading Window in Lateral Flow
Tests Detecting Exposure to Onchocerca volvulus: A New Technology to Improve
Epidemiological Surveillance Tools. PLOS One, 2013. 8:e69231.
Higazi, T.B. and T.R. Unnasch, Biolistic transformation of Brugia malayi. Methods in Molecular
Biology, 2013. 940:103-15.
Higazi, T.B., I.M.A. Zarroug, H.A. Mohamed, W.A. ElMubark, T.C.M. Deran, N. Aziz, M.
Katabarwa, H.K. Hassan, T.R. Unnasch, C.D. Mackenzie, F. Richards, and K. Hashim,
Interruption of Onchocerciasis Transmission in the Abu Hamed Focus, Sudan. American Journal
of Tropical Medicine and Hygiene, 2013. 89:51-57.
Jacob, B.G., R.J. Novak , L. Toe, M. Sanfo, D.A. Griffith, T.L. Lakwo, P. Habomugisha, M.N.
Katabarwa, and T.R. Unnasch, Validation of a remote sensing model to identify Simulium
damnosum s.l. breeding sites in sub-Saharan Africa. PLOS Neglected Tropical Diseases, 2013.
7:e2342.
Jacob , B.G., R.J. Novak , L. Toe, M. Sanfo, L. Yameogo, S. Caliskan, R. Tingueria, A. Pare, and
T.R. Unnasch, Definability of combinatorial functions and their linear recurrence relationships
within a polylogarithmic triangularizable matrix employing surjective bilipschitz functions and
other isomorphisms of metric spaces for forecasting seasonal endemic onchocerciasis
transmission zones. Journal of Pure and Applied Statistics, 2013. 2:401-460.
Jacob , B.G., R.J. Novak , L. Toe, M.S. Sanfo, S. Caliskan, R. Tingueria, A. Pare, L. Yameogo, D.
Griffith, and T.R. Unnasch, Finite-Difference Derivatives of a First-Order Integral Approximation
Quantized with a Default Dual Quasi-Newton Optimizer and a Pseudo-Lipschitzian Property for
Predictive Mapping Spatially Inhomogeneous Simulium damnosum s.l. Explanatory Covariates.
Journal of Statistics: Advances in Theory and Applications, 2013. 10:1-136.
Katabarwa, M.N., T. Lakwo, P. Habomugisha, S. Agunyo, E. Byamukama, D. Oguttu, E.
Tukesiga, D. Unoba, P. Dramuke, A. Onapa, E.M. Tukahebwa, D. Lwamafa, F. Walsh, and T.R.
Unnasch, Transmission of Onchocerca volvulus Continues in Nyagak-Bondo Focus of
Northwestern Uganda after 18 Years of a Single Dose of Annual Treatment with Ivermectin.
American Journal of Tropical Medicine and Hygiene, 2013. 89:293-300.
Lakwo, T.L., R. Garms, T. Rubaale, M. Katabarwa, F. Walsh, P. Habomugisha, D. Oguttu, T.
Unnasch, H. Namanya, E. Tukesiga, J. Katamanywa, J. Bamuhiiga, E. Byamukama, S. Agunyo,
and F. Richards, The disappearance of onchocerciasis from the Itwara focus, Western Uganda
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after elimination of the vector Simulium neavei and 19 years of annual ivermectin treatments.
Acta Tropica, 2013. 126:218-221.
Melnikow, E., S. Xu, J. Liu, A.J. Bell, E. Ghedin, T.R. Unnasch, and S. Lustigman, A potential role
for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and
cytoskeleton in maintenance of endosymbiosis. PLOS Neglected Tropical Diseases, 2013.
7:e2151.
Rodríguez-Pérez, M., A. Domínguez-Vázquez, T.R. Unnasch, H.K. Hassan, J.I. ArredondoJiménez, M.E. Orozco-Algarra, K.B. Rodríguez-Morales, I.C. Rodríguez-Luna, and F.G. PradoVelasco, Interruption of transmission of Onchocerca volvulus in the Southern Chiapas focus,
México. PLOS Neglected Tropical Diseases, 2013. 7:e2133.
Rodríguez-Pérez, M.A., M.A. Adeleke, N.D. Burkett-Cadena, J.A. Garza-Hernández, F. ReyesVillanueva, E.W. Cupp, L. Toé, M.C. Salinas-Carmona, A.D. Rodríguez-Ramírez, C.R. Katholi,
and T.R. Unnasch, Development of a novel trap for the collection of black flies of the Simulium
ochraceum complex. PLOS One, 2013. 8:e76814.
Barrera, R., A. Bingham, H.K. Hassan, M. Amador, A.J. Mackay, and T.R. Unnasch, Vertebrate
Hosts of Aedes aegypti and Aedes mediovittatus (Diptera: Culicidae) in Rural Puerto Rico.
Journal of Medical Entomology, 2012. 49:917-921.
Bingham, A., S.P. Graham, N. Burkett-Cadena, G.S. White, and T.R. Unnasch, Detection of
Eastern Equine Encephalomyelitis virus RNA in North American snakes. American Journal of
Tropical Medicine and Hygiene, 2012. 87:1140-1144.
Burkett-Cadena, N., H.K. Hassan, M.D. Eubanks, E.W. Cupp, and T.R. Unnasch, Winter severity
predicts the timing of host shifts in the mosquito Culex erraticus. Biology Letters, 2012
Estep, L.K., C.J. McClure, N.D. Burkett-Cadena, H.K. Hassan, T.R. Unnasch, and G.E. Hill,
Developing models for the forage ratios of Culiseta melanura and Culex erraticus based on
characteristics of avian hosts. Journal of Medical Entomology, 2012. 49:378-387.
Gopal, H., H.K. Hassan, M.A. Rodríguez-Pérez, L.D. Toé, S. Lustigman, and T.R. Unnasch,
Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR Pool
Screening of Vector Black Flies. PLOS Neglected Tropical Diseases, 2012. 6:e1712.
Graham, S.P., T. Chapman, H.K. Hassan, G. White, C. Guyer, and T.R. Unnasch,
Serosurveillance of eastern equine encephalitis virus in amphibians and reptiles from Alabama,
U.S.A. American Journal of Tropical Medicine and Hygiene, 2012. 86:540-544.
Jacob, B.G., R.J. Novak, L. Toe, M.S. Sanfo, A. Afriyie, M.A. Ibrahim, D.A. Griffith, E.
Mutabakaru, and T.R. Unnasch, Quasi-likelihood techniques in a logistic regression equation for
identifying Simulium damnosum s.l. larval habitats intra-cluster covariates in Togo. Geospatial
Information Science, 2012. 15:1-17.
Katabarwa, M., F. Walsh, P. Habomugisha, T. Lakwo, S. Agunyo, D. Oguttu, T.R. Unnasch, D.
Unoba, E. Byamukama, E. Tukesiga, R. Ndyomugyenyi, and F.O. Richards, Transmission of
onchocerciasis in Wadelai focus of Northwestern Uganda has been interrupted and the disease
eliminated. Journal of Parasitology Research, 2012. 2012:748540.
Liu, C., T. Enright, G. Tzertzinis, and T.R. Unnasch, Identification of genes containing ecdysone
response elements in the genome of Brugia malayi. Molecular and Biochemical Parasitology,
2012. 186:38-43.
Vander Kelen, P.T., J.A. Downs, N. Burkett-Cadena, C.L. Ottendorfer, K.L. Hill, S. Sickerman, J.
Hernandez, J. Jinright, B. Hunt, J. Lusk, V. Hoover, K. Armstrong, R.S. Unnasch, L.M. Stark, and
T.R. Unnasch, Habitat associations of Eastern Equine Encephalitis Transmission in Walton
County Florida. Journal of Medical Entomology, 2012. 49:746-756.
Vander Kelen, P.T., J.A. Downs, L.M. Stark, R.W. Loraamm, J.H. Anderson, and T.R. Unnasch,
Spatial epidemiology of eastern equine encephalitis in Florida. International Journal of Health
Geographics, 2012. 11:47.
Vaughan, J.A., J.O. Mehus, C.A. Brewer, D.K. Kvasager, S. Bauer, J.L. Vaughan, H.K. Hassan,
T.R. Unnasch, and J.A. Bell, Theoretical potential of passerine filariasis to enhnce the enzootic
transmission of West Nile Virus. Journal of Medical Entomology, 2012. 49:1430-1441.
Bailey, M., C. Chauhan, C. Liu, and T.R. Unnasch, The Role of Polymorphisms in the Spliced
Leader Addition Domain in Determining Promoter Activity in Brugia malayi. Molecular and
Biochemical Parasitology, 2011. 176:37-41.
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Burkett-Cadena, N., G.S. White, M.D. Eubanks, and T.R. Unnasch, Winter Biology of Wetland
Mosquitoes as a Focus of Eastern Equine Encephalomyelitis Virus Transmission in Alabama,
USA. Journal of Medical Entomology, 2011. 48:967-973.
Burkett-Cadena, N.D., C.J. McClure, R.A. Ligon, S.P. Graham, C.G. Guyer, G.E. Hill, S.S.
Ditchkoff, M.D. Eubanks, H.K. Hassan, and T.R. Unnasch, Host reproductive phenology drives
seasonal patterns of host use in mosquitoes. PLOS One, 2011. 7:e17681.
Estep, L.K., C.J.W. McClure, N. Burkett-Cadena, Hassan, H.K., T.L. Hicks, T.R. Unnasch, and H.
G.E, A multi-year study of mosquito feeding patterns on avian hosts in a southeastern focus of
eastern equine encephalitis virus. American Journal of Tropical Medicine and Hygiene, 2011.
84:718-726.
Gray, K.M., N.D. Burkett-Cadena, M.D. Eubanks, and T.R. Unnasch, Crepuscular flight activity of
Culex erraticus (Diptera: Culicidae). Journal of Medical Entomology, 2011. 48:167-72.
Higazi, T., I. Zarroug, M. Hanan, M. Wigdan, D. Tong, N. Aziz, A., M. Katabarwa, H.K. Hassan,
T.R. Unnasch, C.D. Mackenzie, and F. Richards, PCR pool screening used to compare the
prevalence of infective black flies in two different Northern Sudan onchocerciasis foci American
Journal of Tropical Medicine and Hygiene, 2011. 84:753-756.
Liu, C., P. Vander Kelen, E. Ghedin, S. Lustigman, and T.R. Unnasch, Analysis of Transcriptional
Regulation of Tetracycline Responsive Genes in Brugia malayi. Molecular and Biochemical
Parasitology, 2011. 180:106-111.
Melnikow, E., S. Xu, J. Liu, L. Li, Y. Oskov, E. Ghedin, T.R. Unnasch, and S. Lustigman,
Interaction of a Wolbachia WSP-like protein with a nuclear-encoded protein of Brugia malayi.
International Journal for Parasitology, 2011. 41:1053-1061.
Oliveira, A., C.R. Katholi, N.D. Burkett-Cadena, H.K. Hassan, S. Kristensen, and T.R. Unnasch,
Temporal Analysis of Feeding Patterns of Culex erraticus in Central Alabama. Vector Borne and
Zoonotic Diseases, 2011. 11:413-421.
White, G., C. Ottendorfer, S. Graham, and T.R. Unnasch, Competency of Reptiles and
Amphibians for Eastern Equine Encephalitis Virus. American Journal of Tropical Medicine and
Hygiene, 2011. 85:421-425.
White, G.S., B.E. Pickett, E.J. Lefkowitz, C.L. Ottendorfer, L.M. Stark, and T.R. Unnasch,
Phylogenetic Analysis of Eastern Equine Encephalitis Virus Isolates from Florida. American
Xu, S., C. Liu, G. Tzertzinis, E. Ghedin, C.C. Evans, R. Kaplan, and T.R. Unnasch, In vivo
transfection of developmentally competent Brugia malayi infective larvae. International Journal for
Parasitology, 2011. 41:355-362.
Burkett-Cadena, N.D., R.A. Ligon, M. Liu, H.K. Hassan, G.E. Hill, M.D. Eubanks, and T.R.
Unnasch, Vector-host interactions in avian nests: do mosquitoes prefer nestlings over adults?
Estep, L., N.D. Burkett-Cadena, G.E. Hill, R.S. Unnasch, and T.R. Unnasch, Estimation of
dispersal distances of Culex erraticus in a focus of Eastern equine encephalitis virus in the
southeastern United States. Journal of Medical Entomology, 2010. 47:977-986.
Hill, G.E., L. Sieferman, M. Liu, H. Hassan, and T.R. Unnasch, The Effects of West Nile Virus on
the Reproductive Success and Overwinter Survival of Eastern Bluebirds in Alabama. Vector
Borne and Zoonotic Diseases, 2010. 10:159-163.
Jacob, B.G., N.D. Burkett-Cadena, J. Luvall, S. Parack, C.J.W. McClure, E. L., G.E. Hill, E.W.
Cupp, R.J. Novak , and T.R. Unnasch, Developing GIS-Based Eastern Equine Encephalitis
Vector-host Models in Tuskegee, Alabama International Journal of Health Geographics, 2010.
9:12.
Liu, C., C. Chauhan, and T.R. Unnasch, The role of local secondary structure in the function of
the trans splicing motif of Brugia malayi. Molecular and Biochemical Parasitology, 2010. 169:115119.
Liu, C., A. Oliveira, C. Chauhan, E. Ghedin, and T.R. Unnasch, Functional Analysis of Putative
Operons in Brugia malayi. International Journal for Parasitology, 2010. 40:63-71.
Rodriguez-Perez, M.A., T.R. Unnasch, A. Dominguez-Vasquez, A.L. Morales-Castro, F.
Richards, G.P. Pena-Flores, E. Orozco-Algarra, and G. Prado-Velasco, Lack of active
Onchocerca volvulus transmission in the Northern Chiapas focus of Mexico. American Journal of
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Rodriguez-Perez, M.A., T.R. Unnasch, A. Dominguez-Vazquez, A.L. Morales-Castro, G.P. PenaFlores, M.E. Algarra-Orozco, J.I. Arredondo-Jimenez, F. Richards, M.A. Vasquez-Rodriguez, and
V.G. Rendon, Interruption of Transmission of Onchocerca volvulus in the Oaxaca Focus, Mexico.
Tzertzinis, G., A.L. Egana, S.R. Palli, M. Robinson-Rechavi, C.R. Gissendanner, C. Liu, T.R.
Unnasch, and C.V. Maina, Molecular evidence for a functional ecdysone signaling system in
Brugia malayi. PLOS Neglected Tropical Diseases, 2010. 4:e625.
Ghedin, E., T. Hailemariam, J. DePasse, X. Zhang, Y. Oksov, T.R. Unnasch, and S. Lustigman,
Brugia malayi gene expression in response to targeted elimination of Wolbachia endosymbiont.
PLOS Neglected Tropical Diseases, 2009. 3:e525.
Graham, S.P., H.K. Hassan, N.D. Burkett-Cadena, C. Guyer, and T.R. Unnasch, Nestedness of
Ectoparasite-Vertebrate Host Networks. PLOS One, 2009. 4:e7873.
Ligon, R.A., N.D. Burkett-Cadena, M. Liu, G.E. Hill, H. Hassan, and T.R. Unnasch, Assessing
Mosquito Feeding Patterns on Nestling and Brooding Adult Birds Using Microsatellite Markers.
Liu, C., C. Chauhan, C.R. Katholi, and T.R. Unnasch, The splice leader addition domain
represents an essential conserved motif for heterologous gene expression in B. malayi. Molecular
and Biochemical Parasitology, 2009. 166:15-21.
O'Conner, L., J.B. Gingrich, T. Unnasch, and H.K. Hassan, Gonotrophic age structure of
mosquitoes in the Culex pipiens complex (Diptera: Culicidae) and possible influences on host
meal selection. Journal of Parasitology and Vector Biology, 2009. 13:25-30.
Ottendorfer, C.L., J.H. Ambrose, G.S. White, T.R. Unnasch, and L.M. Stark, Isolation of genotype
V St. Louis encephalitis virus in Florida. Emerging Infectious Diseases, 2009. 15:604-6.
Ulloa, A., H.H. Ferguson, J.D. Mendez-Sanchez, R. Danis-Lozano, M. Casas-Martinez, J.G.
Bond, J.C. Garcia-Zebadua, A. Orozco-Bonilla, J.A. Juarez-Ordaz, J.A. Farfan-Ale, J.E. GarciaRejon, E.P. Rosado-Paredes, E. Edwards, N. Komar, H.K. Hassan, T.R. Unnasch, and M.A.
Rodriguez-Perez, West Nile Virus Activity in Mosquitoes and Domestic Animals in Chiapas,
Mexico. Vector Borne and Zoonotic Diseases, 2009. 9:555-60.
Burkett-Cadena, N.D., M.D. Eubanks, and T.R. Unnasch, Preference of female mosquitoes for
natural and artificial resting sites. Journal of the American Mosquito Control Association, 2008.
24:228-235.
Burkett-Cadena, N.D., S.P. Graham, H.K. Hassan, C. Guyer, M.D. Eubanks, C.R. Katholi, and
T.R. Unnasch, Blood feeding patterns of potential arbovirus vectors of the genus Culex targeting
ectothermic hosts. American Journal of Tropical Medicine and Hygiene, 2008. 79:809-15.
de Oliveira, A.D., C.R. Katholi, and T.R. Unnasch, Characterization of the promoter of the Brugia
malayi 12kDa small subunit ribosomal protein (RPS12) gene. International Journal for
Parasitology, 2008. 38:1111-1119.
Gu, W., T.R. Unnasch, C.R. Katholi, R. Lampman, and R.J. Novak, Fundamental issues in
mosquito surveillance for arboviral transmission. Transactions of the Royal Society of Tropical
Rodriguez-Perez, M.A., C. Lizarazo-Ortega, H.K. Hassan, A. Dominguez-Vasquez, J. MendezGalvan, P. Lugo-Moreno, M. Sauerbrey, F. Richards, Jr., and T.R. Unnasch, Evidence for
suppression of Onchocerca volvulus transmission in the Oaxaca focus in Mexico. American
Rodriguez-Perez, M.A., M.A. Lutzow-Steiner, A. Segura-Cabrera, C. Lizarazo-Ortega, A.
Dominguez-Vazquez, M. Sauerbrey, F. Richards, Jr., T.R. Unnasch, H.K. Hassan, and R.
Hernandez-Hernandez, Rapid suppression of Onchocerca volvulus transmission in two
communities of the Southern Chiapas focus, Mexico, achieved by quarterly treatments with
Mectizan. American Journal of Tropical Medicine and Hygiene, 2008. 79:239-44.
Cupp, E.W., H.K. Hassan, X. Yue, W.K. Oldland, B.M. Lilley, and T.R. Unnasch, West Nile Virus
Infection in Mosquitoes in the Mid-South USA, 2002-2005. Journal of Medical Entomology, 2007.
44:117-125.
de Oliveira, A., T.R. Unnasch, K. Crothers, S. Eiser, P. Zucchic, J. Moir, C.B. Beard, G.G.
Lawrence, and L. Huang, Performance of a Molecular Viability Assay for the Diagnosis of
Pneumocystis Pneumonia in HIV-Infected Patients. Diagnostic Microbiology and Infectious
Disease, 2007. 57:169-176.
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Dennett, J.A., A. Bala, T. Wuithiranyagool, Y. Randle, C.B. Sargent, H. Guzman, M. Siirin, H.K.
Hassan, M. Reyna-Nava, T.R. Unnasch, R.B. Tesh, R.E. Parsons, and R. Bueno, Jr.,
Associations between two mosquito populations and West Nile virus in Harris County, Texas,
2003-06. Journal of the American Mosquito Control Association, 2007. 23:264-75.
Ghedin, E., S. Wang, D. Spiro, E. Caler, Q. Zhao, J. Crabtree, J.E. Allen, A.L. Delcher, D.B.
Guiliano, D. Miranda-Saavedra, S.V. Angiuoli, T. Creasy, P. Amedeo, B. Haas, N.M. El-Sayed,
J.R. Wortman, T. Feldblyum, L. Tallon, M. Schatz, M. Shumway, H. Koo, S.L. Salzberg, S.
Schobel, M. Pertea, M. Pop, O. White, G.J. Barton, C.K. Carlow, M.J. Crawford, J. Daub, M.W.
Dimmic, C.F. Estes, J.M. Foster, M. Ganatra, W.F. Gregory, N.M. Johnson, J. Jin, R. Komuniecki,
I. Korf, S. Kumar, S. Laney, B.W. Li, W. Li, T.H. Lindblom, S. Lustigman, D. Ma, C.V. Maina, D.M.
Martin, J.P. McCarter, L. McReynolds, M. Mitreva, T.B. Nutman, J. Parkinson, J.M. PeregrinAlvarez, C. Poole, Q. Ren, L. Saunders, A.E. Sluder, K. Smith, M. Stanke, T.R. Unnasch, J.
Ware, A.D. Wei, G. Weil, D.J. Williams, Y. Zhang, S.A. Williams, C. Fraser-Liggett, B. Slatko,
M.L. Blaxter, and A.L. Scott, Draft genome of the filarial nematode parasite Brugia malayi.
Science, 2007. 317:1756-60.
Lindblade, K.A., B. Arana, G. Zea-Flores, N. Rizzo, C.H. Porter, A. Dominguez, N. Cruz-Ortiz,
T.R. Unnasch, G.A. Punkosdy, J. Richards, M. Sauerbrey, J. Castro, E. Catu, O. Oliva, and F.O.
Richards, Jr., Elimination of Onchocerca volvulus transmission in the Santa Rosa focus of
Guatemala. American Journal of Tropical Medicine and Hygiene, 2007. 77:334-41.
Liu, C., A. de Oliveira, T.B. Higazi, E. Ghedin, J. DePasse, and T.R. Unnasch, Sequences
necessary for trans-splicing in transiently transfected Brugia malayi. Molecular and Biochemical
Parasitology, 2007. 156:62-73.
Patrican, L.A., L.E. Hackett, J.E. Briggs, J.W. McGowan, T.R. Unnasch, and J.H. Lee, Hostfeeding patterns of Culex mosquitoes in relation to trap habitat. Emerging Infectious Diseases,
2007. 13:1921-3.
Savage, H.M., D. Aggarwal, C.S. Apperson, C.R. Katholi, E. Gordon, H.K. Hassan, M. Anderson,
D. Charnetzky, L. McMillen, E.A. Unnasch, and T.R. Unnasch, Host choice and West Nile virus
Infection rates in blood fed mosquitoes, including members of the Culex pipiens complex, from
Memphis and Shelby County, Tennessee 2002-2003. Vector Borne and Zoonotic Diseases,
2007. 7:365-386.
Eng, J.K., W.J. Blackhall, M.Y. Osei-Atweneboana, C. Bourguinat, D. Galazzo, R.N. Beech, T.R.
Unnasch, K. Awadzi, G.W. Lubega, and R.K. Prichard, Ivermectin selection on beta-tubulin:
evidence in Onchocerca volvulus and Haemonchus contortus. Molecular and Biochemical
Parasitology, 2006. 150:229-35.
Katholi, C.R. and T.R. Unnasch, Important experimental parameters for determining infection
rates in arthropod vectors using pool screening approaches. American Journal of Tropical
Richards, S.L., L. Ponnusammy, T.R. Unnasch, H.K. Hassan, and C.A. Apperson, Host-feeding
Patterns of Aedes albopictus (Skuse) (Diptera: Culicidae) in relation to the availability of human
and domestic animals in suburban landscapes of central North Carolina with notes on blood meal
hosts of sympatric mosquito species. Journal of Medical Entomology, 2006. 43:543-551.
Rodríguez-Pérez, M.A., C.R. Katholi, H.K. Hassan, and T.R. Unnasch, A large-scale entomologic
assessment of Onchocerca volvulus transmission by pool-screen PCR in Mexico. American
Rodríguez-Pérez, M.A., C.A. Núñez-González, C. Lizarazo-Ortega, A. Sánchez-Varela, M.C.
Wooten, and T.R. Unnasch, Analysis of genetic variation in the ribosomal DNA internal
transcribed spacer and the NADH dehydrogenase subunit 4 mitochondrial genes of the
onchocerciasis vector Simulium ochraceum sensu lato. Journal of Medical Entomology, 2006.
43:701-706.
Unnasch, R.S., T. Sprenger, C.R. Katholi, E.W. Cupp, G.E. Hill, and T.R. Unnasch, A Dynamic
Transmission Model of Eastern Equine Encephalomyelitis Virus. Ecological Modelling, 2006.
192:425-440.
Higazi, T.B., A. DeOliveira, C.R. Katholi, L. Shu, J. Barchue, M. Lisanby, and T.R. Unnasch,
Identification of elements essential for transcription in Brugia malayi promoters. Journal of
Molecular Biology, 2005. 353:1-13.
21
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109.
Higazi, T.B., A. Filiano, C. Katholi, K.Y. Dadzie, J.H. Remme, and T.R. Unnasch, Wolbachia
endosymbiont levels in severe and mild strains of Onchocerca volvulus. Molecular and
Ramirez-Ramirez, A., G. Sanchez-Tejeda, J. Mendez-Galvan, T.R. Unnasch, and A. MonroyOstria, Molecular studies of Onchocerca volvulus isolates from Mexico. Infect Genet Evol, 2005.
22:22.
Adjami, A.G., T. L, Y. Bissan, S. Bugri, L. Yaméogo, M. Kone, C.R. Katholi, and T.R. Unnasch,
The Current Status of Onchocerciasis in the Forest/Savanna Transition Zone of Côte d’ Ivoire.
Parasitology, 2004. 128:407-414.
Apperson, C.S., H.K. Hassan, B.A. Harrison, S.E. Aspen, H.M. Savage, A. Farajollahi, W. Crans,
T.J. Daniels, R.C. Falco, M. Benedict, M. Anderson, L. McMillen, and T.R. Unnasch, Host feeding
patterns of probable vector mosquitoes of West Nile Virus in the eastern United States. Vector
Borne and Zoonotic Diseases, 2004. 4:71-82.
Cupp, E.W., K.J. Tennessen, W.K. Oldland, H.K. Hassan, G.E. Hill, C.R. Katholi, and T.R.
Unnasch, Mosquito and arbovirus activity during 1997-2002 in a wetland in northeastern
Mississippi. Journal of Medical Entomology, 2004. 41:495-501.
Cupp, E.W., D. Zhang, X. Yue, M.S. Cupp, C. Guyer, T. Korves, and T.R. Unnasch, Identification
of reptilian and amphibian bloodmeals from mosquitoes in an Eastern Equine Encephalomyelitis
virus focus in central Alabama. American Journal of Tropical Medicine and Hygiene, 2004.
71:272-276.
Higazi, T.B., A.D. Klion, M. Boussinesq, and T.R. Unnasch, Genetic Heterogeneity in Loa loa
parasites from Southern Cameroon: A Preliminary Study. Filaria Journal, 2004. 3:4.
Higazi, T.B., L. Shu, and T.R. Unnasch, Development and transfection of short term primary cell
cultures from Brugia malayi. Molecular and Biochemical Parasitology, 2004. 137:345-348.
Higazi, T.B. and T.R. Unnasch, Intron encoded sequences necessary for trans splicing in
transiently transfected Brugia malayi. Molecular and Biochemical Parasitology, 2004. 137:181184.
Rodríguez-Pérez, M.A., B.G. Lilley, A. Domínguez-Vázquez, R. Segura-Arenas, C. LizarazoOrtega, A. Mendoza-Herrera, F. Reyes-Villanueva, and T.R. Unnasch, Polymerase Chain
Reaction monitoring of transmission of Onchocerca volvulus in two endemic states in Mexico.
Cupp, E.W., K. Klingler, H.K. Hassan, L.M. Viguers, and T.R. Unnasch, Transmission of eastern
equine encephalomyelitis virus in central Alabama. American Journal of Tropical Medicine and
Hygiene, 2003. 68:495-500.
Davis, C.T., D.W.C. Beasley, H. Guzman, P. Raj, M. D’Anton, R.J. Novak, T.R. Unnasch, R.B.
Tesh, and A.D.T. Barrett, Genetic variation among temporally and geographically distinct West
Nile virus isolates collected in the United States during 2001 and 2002. Emerging Infectious
Diseases, 2003. 9:1423-1429.
Guevara, A.G., J.C. Vieira, B.G. Lilley, A. López, N. Vieira, J. Rumbea, R. Collins, and T.R.
Unnasch, Entomolological Evaluation by Pool Screen Polymerase Chain Reaction of Onchocerca
volvulus Transmission in Ecuador Following Mass Mectizan Distribution. American Journal of
Hassan, H.K., E.W. Cupp, G.E. Hill, C.R. Katholi, K. Klingler, and T.R. Unnasch, Avian host
preference by vectors of Eastern Equine Encephalomyelitis virus. American Journal of Tropical
Higazi, T.B., E. Pearlman, D.R. Whikehart, and T.R. Unnasch, Angiogenic activity of an
Onchocerca volvulus Ancylostoma secreted protein homologue. Molecular and Biochemical
Huang, L., K. Crothers, A. DeOliveira, S. Eiser, P. Zucchi, C.B. Beard, and T.R. Unnasch,
Application of an mRNA-based molecular viability assay to oropharyngeal washes for the
diagnosis of Pneumocystis pneumonia in HIV-infected patients. A pilot study. J Eukaryot
Microbiol., 2003. 50:618-20.
Shu, L., C. Katholi, T. Higazi, and T.R. Unnasch, Analysis of the Brugia malayi HSP70 promoter
using a homologous transient transfection system. Molecular and Biochemical Parasitology,
2003. 128:67-75.
22
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Yung, S.C., T.R. Unnasch, and N. Lang-Unnasch, Cis and trans factors involved in apicoplast
targeting of Toxoplasma gondii. Journal of Parasitology, 2003. 89:767-776.
Apperson, C.S., B.A. Harrison, T.R. Unnasch, H.K. Hassan, W.S. Irby, H.M. Savage, S.E. Aspen,
D.W. Watson, L.M. Rueda, B.R. Engber, and R.S. Nasci, Host-feeding habits of Culex and other
mosquitoes (Diptera: Culicidae) in the borough of Queens in New York City, with characters and
techniques for identification of Culex mosquitoes. Journal of Medical Entomology, 2002. 39:777785.
Higazi, T.B., A. Merriweather, L. Shu, R. Davis, and T.R. Unnasch, Brugia malayi: Transient
transfection by microinjection and particle bombardment. Experimental Parasitology, 2002.
100:95-102.
Lee, J.H., H. Hassan, G. Hill, E.W. Cupp, T.B. Higazi, C.J. Mitchell, M.S. Godsey, and T.R.
Unnasch, Identification of mosquito avian-derived blood meals by polymerase chain reactionheteroduplex analysis. American Journal of Tropical Medicine and Hygiene, 2002. 66:599-604.
Lee, J.H., K. Tennessen, B.H. Lilley, and T.R. Unnasch, Simultaneous Detection of Three
Mosquito-borne Encephalitis Viruses (Eastern Equine, LaCrosse and St. Louis) Using a Single
Tube Multiplex Reverse Transcription-PCR Assay. Journal of the American Control Association,
2002. 18:26-31.
Maher, N., H.K. Dillon, A. Awooda, J.H. Lee, S.H. Vermund, and T.R. Unnasch, A Comparison of
two Surface Sample Collection Devices for Use in Polymerase Chain Reaction Based Detection
of Pneumocystis carinii in House Dust. Applied Occupational and Environmental Hygiene, 2002.
17:416-423.
Oyibo, W.A., F.A. Fagbenro-Beyioku, A. Merriweather, and T.R. Unnasch, Molecular
characterization of Onchocerca volvulus in a middlebelt state of Nigeria. African Journal of
Science and Technology, 2002. 3:22-24.
Higazi, T.B., C.R. Katholi, B.M. Mahmoud, O.Z. Baraka, M.M. Mukhtar, Y. Al Qubati, and T.R.
Unnasch, Onchocerca volvulus: Genetic Diversity of Parasite Isolates from Sudan. Experimental
Maher, N., H.K. Dillon, S.H. Vermund, and T.R. Unnasch, Magnetic bead capture eliminates PCR
inhibitors in samples collected from the airborne environment, permitting detection of
Pneumocystis carinii DNA. Applied and Environmental Microbiology, 2001. 67:449-452.
Maher, N.H., S.H. Vermund, D.A. Welsh, H.K. Dillon, A. Awooda, and T.R. Unnasch,
Development and Characterization of a Molecular Viability Assay for Pneumocystis carinii f. sp.
hominis. Journal of Infectious Diseases, 2001. 183:1825-1827.
Merriweather, A., V. Guenzler, M. Brenner, and T.R. Unnasch, Characterization and expression
of enzymatically active recombinant filarial prolyl 4-hydroxylase. Molecular and Biochemical
Parasitology, 2001. 116:185-97.
Yung, S., T.R. Unnasch, and N. Lang-Unnasch, Analysis of apicoplast targeting and transit
peptide processing in Toxoplasma gondii by deletional and insertional mutagenesis. Molecular
Higazi, T.B., D.A. Boakye, M.D. Wilson, B.M. Mahmoud, O.Z. Baraka, M.M. Mukhtar, and T.R.
Unnasch, Cytotaxonomic and Molecular Analysis of (Simulium (Edwardsellum) damnosum
Theobald sensu lato from Abu Hamed, Sudan. Journal of Medical Entomology, 2000. 37:547-553.
Maher, N., S. Vermund, M. Lasbury, C.H. Lee, M. Bartlett, and T.R. Unnasch, Development and
evaluation of a molecular viability assay for Pneumocystis carinii. Journal of Clinical Microbiology,
2000. 38:1947-1952.
Simon, C., J. Tang, S. Dalwadi, G. Staley, J. Deniega, and T.R. Unnasch, Allochronic speciation
suggested by restricted gene flow between 13-year cicadas and "17-year cicadas with 13-year
life cycles". Evolution, 2000. 54:1326-1336.
Tawe, W., E. Pearlman, T.R. Unnasch, and S. Lustigman, Angiogenic activity of Onchocerca
volvulus recombinant proteins similar to vespid venom antigen 5. Molecular and Biochemical
Toe, L., A.G. Adjami, B.A. Boatin, C. Back, E.S. Alley, N. Dembele, P.G. Brika, E. Pearlman, and
T.R. Unnasch, Topical application of diethylcarbamazine to detect onchocerciasis recrudescence
in west Africa. Transactions of the Royal Society of Tropical Medicine and Hygiene, 2000.
94:519-25.
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141.
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143.
144.
Boakye, D., J.M. Tang, P. Truc, A. Merriweather, and T.R. Unnasch, Identification of blood meals
in hematophagous diptera by polymerase chain reaction amplification and heteroduplex analysis.
Medical and Veterinary Entomology, 1999. 13:282-287.
Graham, S.P., Y. Wu, K. Henkle-Duehrsen, S. Lustigman, T.R. Unnasch, G. Braun, S.A.
Willimas, J. McCarthy, A.J. Trees, and A.E. Bianco, Patterns of Onchocerca volvulus recombinant
antigen recognition in a bovine model of onchocerciasis. Parasitology, 1999. 119:603-612.
Keddie, E.M., T. Higazi, D. Boakye, A. Merriweather, M.C. Wooten, and T.R. Unnasch,
Onchocerca volvulus: Limited heterogeneity in the nuclear and mitochondrial genomes.
Experimental Parasitology, 1999. 93:198-206.
Ogunrinade, A., D. Boakye, A. Merriweather, and T.R. Unnasch, Distribution of the blinding and
non-blinding strains of Onchocerca volvulus in Nigeria. Journal of Infectious Diseases, 1999.
179:1577-1579.
Pearlman, E., L. Toe, B.A. Boatin, A.A. Gilles, A.W. Higgins, and T.R. Unnasch, Eotaxin
expression in Onchocerca volvulus-induced dermatitis after topical application of
diethylcarbamazine. Journal of Infectious Diseases, 1999. 180:1394-1397.
Rodríguez-Pérez, M.A., R. Danis-Lozano, M.H. Rodríguez, T.R. Unnasch, and J.E. Bradley,
Detection of Onchocerca volvulus infection in Simulium ochraceum sensu lato: Comparison of a
PCR assay and fly dissection in a Mexican hypoendemic community. Parasitology, 1999.
119:613-619.
Unnasch, T.R., J. Bradley, J. Beauchamp, R. Tuan, and M.W. Kennedy, Characterization of a
putative nuclear receptor from Onchocerca volvulus. Molecular and Biochemical Parasitology,
1999. 104:259-269.
Yameogo, L., L. Toe, J.M. Hougard, B.A. Boatin, and T.R. Unnasch, Pool screen polymerase
chain reaction for estimating the prevalence of Onchocerca volvulus infection in Simulium
damnosum sensu lato: results of a field trial in an area subject to successful vector control.
Keddie, E.M., T. Higazi, and T.R. Unnasch, The mitochondrial genome of Onchocerca volvulus:
Sequence, structure and phylogenetic analysis. Molecular and Biochemical Parasitology, 1998.
95:111-127.
Tang, J., J.K. Moulton, K. Pruess, E.W. Cupp, and T.R. Unnasch, Genetic variation in North
American black flies in the subgenus Psilopelmia (Diptera:Simuliidae). Canadian Journal of
Zoology, 1998. 76:205-211.
Toe, L., B.A. Boatin, A. Adjami, C. Back, A. Merriweather, and T.R. Unnasch, Detection Of
Onchocerca volvulus Infection By O-150 Polymerase Chain Reaction Analysis Of Skin Scratches.
Journal of Infectious Diseases, 1998. 178:282-285.
Pearlman, E., E. Diaconu, F.E. Hazlett, A. Merriweather, and T.R. Unnasch, Identification of an
epitope of a recombinant Onchocerca volvulus protein that induces corneal pathology. Molecular
Tang, J. and T.R. Unnasch, Heteroduplex analysis in medical entomology: A rapid and sensitive
sequence-based tool for population and phylogenetic studies. Parasitology Today, 1997. 13:271274.
Toe, L., C. Back, A.G. Adjami, J.M. Tang, and T.R. Unnasch, Onchocerca volvulus: Comparison
of field collection methods for the preservation of parasite and vector samples for PCR analysis.
Bulletin of the World Health Organization, 1997. 75:443-447.
Toe, L., J. Tang, C. Back, C.R. Katholi, and T.R. Unnasch, Vector-Parasite transmission
complexes for onchocerciasis in West Africa. The Lancet, 1997. 349:163-166.
Chakravarti, B., B.M. Greene, S.K. Dey, J.S. Parker, T.R. Unnasch, T.A. Herring, A. Gbakima,
M.L. Eberhard, and D.N. Chakravarti, Onchocerca volvulus: Expression and Purification of
Recombinant Antigen RAL-2 - Studies on Immunogenicity and Pathogenicity. Biochemical
Archives, 1996. 12:55-69.
Katholi, C.R., A. Merriweather, and T.R. Unnasch, An Analysis of Variance Type Test for
Comparing Clusters of DNA Sequences Based on Randomization Test Methodologies. Journal of
Applied Statistics, 1996. 24:371-381.
Merriweather, A. and T.R. Unnasch, Onchocerca volvulus: Development of a species specific
polymerase chain reaction assay. Experimental Parasitology, 1996. 83:164-166.
24
145.
Tang, J., K. Pruess, E.W. Cupp, and T.R. Unnasch, Molecular phylogeny and typing of blackflies
(Diptera:Simuliidae) that serve as vectors of human or bovine onchocerciasis. Medical and
Veterinary Entomology, 1996. 10:228-234.
146.
Tang, J., L. Toe, C. Back, and T.R. Unnasch, Intra-specific Heterogeneity of the rDNA Internal
Transcribed Spacer in the Simulium damnosum (Diptera:Simuliidae) Complex. Molecular Biology
and Evolution, 1996. 13:244-252.
147.
Tang, J., K. Pruess, and T.R. Unnasch, Genotyping North American black flies by means of
mitochondrial ribosomal RNA sequences. Canadian Journal of Zoology, 1996
. 74:39-46.
148.
Yates, R.A., R.S. Tuan, K.J. Shepley, and T.R. Unnasch, Characterization of genes encoding
members of the nuclear hormone receptor superfamily from Onchocerca volvulus. Molecular and
149.
Katholi, C.R., L. Toe, A. Merriweather, and T.R. Unnasch, Determining the prevalence of
Onchocerca volvulus infection in vector populations by polymerase chain reaction screening of
pools of black flies. Journal of Infectious Diseases, 1995. 172:1414-7.
150.
Tang, J. and T.R. Unnasch, Discriminating PCR artifacts using DHDA (directed heteroduplex
analysis). BioTechniques, 1995. 19:902-905.
151.
Tang, J., L. Toe, C. Back, and T.R. Unnasch, Mitochondrial alleles of Simulium damnosum sensu
lato infected with Onchocerca volvulus. International Journal for Parasitology, 1995. 25:1251-4.
152.
Zimmerman, P.A., M. Shapiro, J. Tang, T.B. Nutman, and T.R. Unnasch, Optimizing probe
selection in directed heteroduplex analysis using HDProbe 1.1. BioTechniques, 1995. 19:972977.
153.
Tang, J., L. Toe, C. Back, P.A. Zimmerman, K. Pruess, and T.R. Unnasch, The Simulium
damnosum species complex: phylogenetic analysis and molecular identification based upon
mitochondrially encoded gene sequences. Insect Molecular Biology, 1995. 4:79-88.
154.
Raghavan, N., D.O. Freedman, P.C. Fitzgerald, T.R. Unnasch, E.A. Ottesen, and T.B. Nutman,
Cloning and characterization of a potentially protective chitinase-like recombinant antigen from
Wuchereria bancrofti. Infection and Immunity, 1994. 62:1901-1908.
155.
Toe, L., A. Merriweather, and T.R. Unnasch, DNA probe based classification of Simulium
damnosum s.l. borne and human derived filarial parasites in the Onchocerciasis Control
Programme area. American Journal of Tropical Medicine and Hygiene, 1994. 51:676-683.
156.
LeBlanc, C., C.R. Katholi, T.R. Unnasch, and S.I. Hruska. DNA Sequence Analysis using
Hierarchical ART-based Classification Networks. in Proceedings of the 2nd International
Conference on Intelligent Systems in Molecular Biology. 1994. Stanford University.
157.
Rokeach, L.A., P.A. Zimmerman, and T.R. Unnasch, Epitopes of the Onchocerca volvulus RAL-1
antigen, a member of the calreticulin family of proteins, recognized by sera from patients with
onchocerciasis. Infection and Immunity, 1994. 62:3696-3704.
158.
Pearlman, E., J.W. Kazura, D.S. Bardenstein, T.R. Unnasch, F. Perler, E. Diaconu, F.E. Hazlett,
and J.H. Lass, Induction of Stromal Keratitis by Recombinant Onchocerca volvulus proteins
Ov33, RAL2 and RAL6, in Advances in Ocular Immunology, R.B. Nussenblatt, et al., Editors.
1994, Elsevier: Amsterdam. p. 289-292.
159.
Zhou, Y., E. Dziak, T.R. Unnasch, and M. Opas, Major retinal cell components recognized by
onchocerciasis sera are associated with the cell surface and nucleoli. Investigative Ophthamology
and Vision Science, 1994. 35:1089-1099.
160.
Wilson, W.R., R.S. Tuan, K.J. Shepley, D.O. Freedman, B.M. Greene, K. Awadzi, and T.R.
Unnasch, The Onchocerca volvulus homologue of the multifunctional polypeptide protein disulfide
isomerase. Molecular and Biochemical Parasitology, 1994. 68:103-117.
161.
Zimmerman, P.A., C.R. Katholi, M.C. Wooten, N. Lang-Unnasch, and T.R. Unnasch, Recent
evolutionary history of American Onchocerca volvulus, based on analysis of a tandemly repeated
DNA sequence family. Molecular Biology and Evolution, 1994. 11:384-392.
162.
Freedman, D.O., T.R. Unnasch, A. Merriweather, and K. Awadzi, Truly infection free persons are
rare in areas hyperendemic for African onchocerciasis. Journal of Infectious Diseases, 1994.
170:1054-1055.
163.
Zimmerman, P.A., L. Toe, and T.R. Unnasch, Design of Onchocerca DNA probes based upon
analysis of a repeated sequence family. Molecular and Biochemical Parasitology, 1993. 58:259269.
25
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177.
178.
179.
180.
181.
182.
Bradley, J.E., R.S. Tuan, K.J. Shepley, T.I.M. Tree, R.M. Maizels, R. Helm, W.F. Gregory, and
T.R. Unnasch, Onchocerca volvulus: Characterization of an immunodominant antigen present in
adult and larval parasites. Experimental Parasitology, 1993. 77:414-424.
Kron, M.A., K. Erttmann, B.M. Greene, and T. Unnasch, Characterization of a possible tRNA
synthetase gene from Onchocerca volvulus. Molecular and Biochemical Parasitology, 1992.
52:289-92.
Zimmerman, P.A., K.Y. Dadzie, G. DeSole, J. Remme, E.S. Alley, and T.R. Unnasch,
Onchocerca volvulus DNA probe classification correlates with epidemiological patterns of
blindness. Journal of Infectious Diseases, 1992. 165:964-968.
Unnasch, T.R., C.R. Katholi, and L.M. Coate, Onchocerca volvulus: Frequency of codon usage.
Experimental Parasitology, 1992. 75:457-459.
Rokeach, L.A., J.A. Haselby, J.F. Meilof, R.J.T. Smeenk, T.R. Unnasch, B.M. Greene, and S.O.
Hoch, Characterization of the Autoantigen Calreticulin. Journal of Immunology, 1991. 147:30313039.
Meredith, S.E.O., G. Lando, A.A. Gbakima, P.A. Zimmerman, and T.R. Unnasch, Onchocerca
volvulus: Application of the Polymerase Chain Reaction to Identification and Strain Differentiation
of the Parasite. Experimental Parasitology, 1991. 73:335-344.
Zimmerman, P.A., M.L. Spell, J. Rawls, and T.R. Unnasch, Transformation of DNA sequence
data into geometric symbols. Biotechniques, 1991. 11:50-52.
Erttmann, K.D., S.E.O. Meredith, B.M. Greene, and T.R. Unnasch, Isolation and characterization
of form specific DNA sequences of Onchocerca volvulus. Acta Leidensia, 1990. 59:253-260.
Joseph, J.T., S.M. Aldritt, T.R. Unnasch, O. Puijalon, and D.F. Wirth, Characterization of a
conserved extra chromosomal element isolated from the avian malaria parasite Plasmodium
gallinaceum. Molecular and Cellular Biochemistry, 1989. 9:3621-3629.
Meredith, S.E.O., T.R. Unnasch, M. Karam, W.F. Piessens, and D.F. Wirth, Cloning and
characterization of an Onchocerca volvulus specific DNA sequence. Molecular and Biochemical
Gallin, M.Y., M. Tan, M.A. Kron, D. Rechnitzer, B.M. Greene, H.S. Newland, A.T. White, H.R.
Taylor, and T.R. Unnasch, Onchocerca volvulus recombinant antigen: Physical characterization
and clinical correlates with serum reactivity. Journal of Infectious Diseases, 1989. 160:521-529.
Waters, A.P., T.R. Unnasch, D.F. Wirth, and T.F. McCutchan, Sequence of a small ribosomal
RNA gene from Plasmodium lophurae. Nucleic Acids Research, 1989. 17:1763.
Unnasch, T.R., M.Y. Gallin, P.T. Soboslay, K.D. Erttmann, and B.M. Greene, Isolation and
characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective
larvae. Journal of Clinical Investigation, 1988. 82:262-269.
Erttmann, K.D., T.R. Unnasch, B.M. Greene, E.J. Albiez, J. Boateng, A.M. Denke, J.J. Ferraroni,
M. Karam, H. Schulz-Key, and P.N. Williams, A DNA sequence specific for forest form
Onchocerca volvulus. Nature, 1987. 327:415-417.
Shah, J., L. Lamontaigne, T.R. Unnasch, D.F. Wirth, and W.F. Piessens, Characterization of a
ribosomal DNA clone from Brugia malayi. Molecular and Biochemical Parasitology, 1986. 19:6775.
Unnasch, T.R., M. McLafferty, and D.F. Wirth, The primary structure of the rRNA insertions of
Plasmodium lophurae. Molecular and Biochemical Parasitology, 1985. 16:149-161.
Unnasch, T.R. and D.F. Wirth, The avian malaria Plasmodium lophurae has a small number of
heterogeneous ribosomal RNA genes. Nucleic Acids Research, 1983. 11:8443-8459.
Unnasch, T.R. and D.F. Wirth, The cloned rRNA genes of Plasmodium lophurae: A novel rDNA
structure. Nucleic Acids Research, 1983. 11:8461-8472.
Unnasch, T.R. and G.M. Brown, Purification and properties of dihydropterin oxidase from
Drosophila melanogaster. Journal of Biological Chemistry, 1982. 257:14211-14216.
Book Chapters, Reviews and Editorials:
183.
184.
Evans, D., T.R. Unnasch, and F.O. Richards, Co-implementation of onchocerciasis and lymphatic
filariasis elimination in Africa: The time is now. The Lancet, 2015. 385:2151-2152.
Rodrıguez-Perez, M.A., E.E. Lara-Ramırez, O. Real-Najarro, and T.R. Unnasch, A Roadmap
Followed: The Path Towards the Elimination of Onchocerciasis in Latin America, in Neglected
26
185.
186.
187.
188.
189.
190.
191.
192.
193.
194.
195.
196.
197.
198.
199.
200.
201.
202.
203.
Tropical Diseases - Latin America and the Caribbean, C. Franco-Paredes and J.I. SantosPreciado, Editors. 2015, Springer-Verlag: Vienna. p. 156-173.
Gargano, L.M., J. Engel, G.C. Gray, K. Howell, J. T.F., W.K. Milhous, J.G. Morris, D.G. Mead, C.
Rubin, T.R. Unnasch, C.W. Woods, and J.M. Hughes, Arbovirus diseases, southeastern United
States. Emerging Infectious Diseases, 2013. 19:
Lok, J.B. and T.R. Unnasch, Transgenesis in animal parasitic nematodes: Strongyloides spp. and
Brugia spp., in WormBook, T.C.e.R. Community, Editor. 2013.
Cupp, E.W., C.D. Mackenzie, and T.R. Unnasch, The importance of ivermectin to human
onchocerciasis: past, present and future. Research and Reports in Tropical Medicine, 2011.
2:81-92.
Rodríguez-Pérez, M.A., U. T., and R.-N. O., Assessment and monitoring of onchocerciasis in
Latin America. Advances in Parasitology, 2011. 77:177-197.
Klempner, M.S., T.R. Unnasch, and L.T. Hu, Taking a bite out of vector-transmitted infectious
diseases. New England Journal of Medicine, 2007. 356:2567-9.
Unnasch, R.S., E.W. Cupp, and T.R. Unnasch, Host selection and its role in transmission of
arboviral encephalitides, in Disease Ecology: Community Structure and Pathogen Dynamics, S.K.
Collinge and C. Ray, Editors. 2005, Oxford University Press: Oxford, UK. p. 73-89.
Unnasch, T.R., Onchocerca volvulus, in Encyclopedia of Diagnostic Genomics and
Proteonomics, J.a.P.M. Fuchs, Editor. 2005, Marcel Dekker, Inc.: New York, NY. p. 943-946.
Beard, C.B., P. Roux, G. Nevez, P.M. Hauser, J.A. Kovacs, T.R. Unnasch, and B. Lundgren,
Strain Typing Methods and Molecular Epidemiology of Pneumocystis Pneumonia. Emerging
Infectious Diseases, 2004. 10:1729-1735.
Unnasch, T.R., River Blindness. The Lancet, 2002. 360:182-183.
Williams, S.A., S.J. Laney, M. Lizotte-Waniewski, L.A. Bierwert, and T.R. Unnasch, The river
blindness genome project. Trends in Parasitology, 2002. 18:86-90.
Unnasch, T.R. and S.A. Williams, The genomes of Onchocerca volvulus. International Journal for
Parasitology, 2000. 30:543-552.
Bradley, J.E. and T.R. Unnasch, Molecular approaches to the diagnosis of onchocerciasis, in
Advances in Parasitology, J.R. Baker, R. Muller, and D. Rollinson, Editors. 1996, Academic
Press: New York. p. 57-106.
Unnasch, T.R., The 0-150 PCR: A New Breed of Test. Mectizan Program Notes, 1996. 15:2-3.
Unnasch, T.R. and S.E.O. Meredith, The use of degenerate primers in conjunction with strain and
species oligonucleotides to classify Onchocerca volvulus, in Species Diagnostic Protocols: PCR
and other nucleic acid methods, J.P. Clapp, Editor. 1996, Humana Press: Totowa, NJ. p. 293303.
Unnasch, T.R. and P.A. Zimmerman, Systematics: Questions and Approaches for the Molecular
Parasitologist, in Molecular Approaches to Parasitology, J.C. Boothroyd and R. Komuniecki,
Editors. 1995, Wiley-Liss: New York. p. 5-20.
Unnasch, T.R., The Filarial Genome Project. Parasitology Today, 1994. 10:415-416.
Unnasch, T.R., DNA probes to identify Onchocerca volvulus. Parasitology Today, 1987. 3:377378.
Greene, B.M. and T.R. Unnasch, Molecular biological approaches to research in onchocerciasis.
Journal of Infectious Diseases, 1986. 154:1024-1026.
Brown, G.M., G.G. Krivi, C.L. Fan, and T.R. Unnasch, The biosynthesis of pteridines in
Drosophila melanogaster, in The Chemistry and Biology of Pteridines, R. Kisliuk and G.M. Brown,
Editors. 1979. p. 81-86.
Thesis:
204.
Unnasch, T.R., Purification and Properties of dihydropterin oxidase from Drosophila
melanogaster. 1981, Massachusetts Institute of Technology.
27

Thomas Raymond Unnasch - USF Health

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