A Randomized Controlled Clinical Study of Karanjadhya Ghrita in

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ISSN : 2249-5746
International Journal of Ayurvedic and Herbal Medicine 5:3 (2015) 1745–1753
Journal homepage: http://www.interscience.org.uk
A Randomized Controlled Clinical Study of Karanjadhya Ghrita in
the management of Dushta Vrana (chronic wounds).
Dr Neelam Choudhary1, Dr Prashant Soni2, Dr Madhusudan Swarnkar3
1
MD (Rasa Shastra & Bhaishajya Kalpana)
Affiliation - P.G. Department of Rasashastra & Bhaishajya Kalpana,
National Institute of Ayurveda, Jaipur 302002
Mail ID – drneelamchoudhary@rediffmail.com
2
MBBS, MBA
Affiliation – National Advisor (Quality Improvement), USAID ASSIST Project, University Research
Company, Delhi.
Email ID – drprashantsoni@yahoo.com
3MD (Community Medicine)
Affiliation – Associate Professor, Dept. of Community Medicine,
Jhalawar Medical College, Jhalawar.
Email ID – swarnkarmadhusudan@gmail.com
Corresponding author:
1. Dr Neelam Choudhary, MD (Rasa Shastra & Bhaishajya Kalpana)
Affiliation - P.G. Department of Rasashastra & Bhaishajya Kalpana,
National Institute of Ayurveda, Jaipur 302002
Mail ID – drneelamchoudhary@rediffmail.com
Abstract:
Background: Dushta Vrana (Chronic wounds) are frequently encountered problem in the present era
produced due to the complications of trauma or pathological injury and it causes long-term agony to the
patients. Chakradutt has mentioned the wound healing properties of Karanjadhya Ghrita. In the present study
Karanjadhya Ghrita was prepared and its wound healing properties were studied on various chronic wounds.
Methods:
Karanjadhya Ghrita was prepared using the standardization procedure the effects were studied on 40 patients
with chronic wounds (wounds more than 3 weeks old) selected from Outpatient and inpatient department of
department of Shalya Tantra, National Institute of Ayurveda, Jaipur. The patients were divided into two
groups – Group A (Karanjadhya Ghrita dressing) and Group B (Hydrogen peroxide and EUSOL and dry
Gauze bandage). The drug was applied topically and patients were studied daily for 30 days and results were
analyzed.
Results:
In Chronic Wound the size was decreased 69.23% which was statistically highly significant. There was
considerable improvement (> 80 %) in swelling and unhealthy margin. Pain, tenderness, discharge and
unhealthy granulation tissue showed moderate effect (between 60 to 70 percent) in the chronic wounds
Conclusion:
From the studies made from clinical cases, it was concluded that, the drug “Karanjadhya Ghrita” possess
sufficient efficacy in Vrana Shodhan and Vrana Ropana properties without producing any adverse effects.
International journal of ayurvedic & herbal medicine 5(3) May-June. 2015(1745-1753)
The drug also increase the rate of contraction produces healthier granulation tissue and rapid healing time.
Hence, this study conclude that application of Karanjadhya Ghrita is highly effective on chronic wounds.
Key Words: Karanjadhya Ghrita, Chronic Wounds, Wound healing
INTRODUCTION
Ayurveda is a science of life and is believed to exist as long as the origin of life on this earth as is mentioned
in Charak Samhita. Ever since the life originated, human being has been susceptible to injury, which made
him to think about healing from very early stage of development. The
destruction/break/rupture/discontinuity of body tissue/part of body, is called “Vrana”1. It may have features
of chronic in nature, foul smell, loss of normal colors, excessive discharge of pus and impure blood,
excessive pain, elevated from surface, irregular shape with cavities, everted base and margins of wound
(Charak Chikitsa 25/832, Madhav Nidan 42/73, Vaidyaksabdasindhu4).In Ayurveda, particularly Sushruta
has mentioned various types of vrana5 and their management6, which is of prime importance in any surgical
practice/procedure. Wound management is a significant and growing health burden on the community7.
Delayed wound healing and wound infection place a substantial financial burden on health care systems, as
a result of increasing dependency and increased hospital admissions. Chronic wounds also have a very large
social and quality of life impact on individuals and carers8. After injury, healing is a natural phenomenon
and continues in sequential manner until the formation as a healthy scar. Usually defense mechanisms of
body takes complete care in order to keep the scar clean. But at times, when infection is massive, surface
area of wound is very large and slough or necrotic tissue is too much, this auto cleansing mechanism
becomes inadequate9. According to Acharya Sushruta among the 60 measures of wound treatment, “Kalka”
(application of paste) is a measure indicated for non-healing wounds located in muscle and having slough.
The paste performs both functions of “Shodhana” (cleaning) and “Ropana” (healing) in Dushta Vrana10. A
close study of Ayurveda reveals that a number of plants were used to achieve the goal Vrana-Ropana.
Acharyas have given beautiful description about wound and wound healing. For good healing, the drug must
have three following properties:
1. Vrana Shodhana
3. Vrana Ropana
It is important to find a single and simple composition, which have these two properties viz.
Shodhan, and Vrana ropana properties. In the present study Karanjadhya Ghrita11, 12 is selected to study its
effects on chronic wounds that described in Chakrdutt Ratanprbha, Vranshoth Rogadhikar.
AIMS AND OBJECTIVES:
To evaluate the efficacy of Karanjadhya Ghrita in the management of chronic wounds.
MATERIALS AND METHODS The work was carried out in the department of Rasa Shastra & Bhesaj Kalpana, National Institute of
Ayurveda, Jaipur.
Drug Contents –
Karanjadhya Ghrita11, 12 contains:Nakta mala (leaves and tender fruits) , Maltipatra (leaves) , Patola (leaves,)Arista (leaves) ,
Haridra ,Daruharidra ,Madhuka, Tikta rohini ,Manjistha ,Candana ,Krisna sariva , Swet , Sariva ,Trivrit ,
Utpala, Ushira. Bee-wax, gau Ghrita.
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Method of Preparation –
The Karanjadhya Ghrita was prepared in the National Institute of Ayurveda Pharmacy under the guidance of
Dr V Nageshwar Rao, Associate Professor and Guide, Department of Rasa Shastra & Bhesaj Kalpana. For
the preparation of medicated oils/ghritas, following steps were done:First the Ghrita has to be subjected to MURCHANA13 and then followed by following steps to prepare the
Karanjadhya Ghrita.
Procedure:
a) Wash and grind fresh leaves of ingredients 1 to 3 of the formulation composition (Kalka dravya) in a wet
grinder and convert in paste form. And, all other Kalka dravyas were made into the powder form.
b) The paste and powder of Kalka drugs are mixed with water and bolus is prepared. Madhuchista keep
aside for addition during snehapāka. Murchita Ghrita was taken in a steel vessel and heated over Madhyam
agni till complete evaporation of moisture content, at this stage temperature gone up to 130° C. The bolus of
Kalka were added to the Ghrita after slight cooling, and constant stirring is carried.
c) Then four times quantity of water to ghrita is added to the above vessel.
d) It is kept over (Mandagni) fire and slowly stirred to mix well.
e) After 3 hrs. and 40 minutes heating it is allowed for self-cooling and a plate is covered to prevent from
any dust fall.
f) On the next day, heating is again continued and 2½ hrs. heating is carried on this day and constant stirring
is carried to avoid sticking of the Kalka drugs
g) On 3rd day, the heating process is continued till the ghirta becomes water free and gets the Laksanas of
Snehasiddhi. Stop heating when the kalka breaks down into pieces on attempting to form a varti and the
froth subsides, the vessel is taken out from the fire and Filter while hot (about 70 0) through a clean cloth.
Add small pieces of Siktha, in the warm-stage and filter through clean cloth and allow to cool.
h) Obtained Karanjadhya Ghrita is preserved in tightly closed glass jar to protect from light and moisture.
Clinical Study:Source of data: For the study 40 patients were randomly selected from the Out-patient and in-patient
departments of Departments of Shalya Tantra and Kaya Chikitsa, National Institute of Ayurveda, Jaipur.
Method of collection of data: Detailed clinical history and clinical examination was carried out using the
special Performa which was prepared before assessing the case and starting the proper treatment with trial
drugs.
Grouping: The patients were randomly divided into two groups, each group having 20 patients – Group A
(Karanjadhya Ghrita dressing) and Group B (Hydrogen peroxide and EUSOL and dry Gauze bandage).
After selection of patients, they were informed about the study and their written consent was obtained.
Further, the patients were made to understand that they are able to withdraw from the study any time without
prior notice.
Intervention: The Group A patients were asked to apply the Karanjadhya Ghrita locally on the wounds two
times a day for 30 days and compared with the Group B patients.
Duration of study: The duration of study was 30 days.
Follow up: All the out-patients were asked to follow-up at the interval of every 10 days in 30 days. All IPD
patients, who were included in study, were studied daily for 30 days.
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Inclusion Criteria: All types of chronic wounds (wounds more than 3 weeks old) patients including
lacerated wound ,Post-operative wounds ,Fissure, Burn, Pressure Sores, Trophic ulcer, Varicose ulcer were
selected.
Exclusion Criteria: Diabetic patients, malignant ulcers, HIV and TB patients, patients suffering from
hepatitis and patients with signs of gangrene.
Assessment Criteria: The wounds were assessed using the following parameters.
1. Symptom Rating Scale:
0-No Sign & symptoms
1-Mild Sign & symptoms
2-Moderate Sign & symptoms
3- Severe Sign & symptoms
2. Assessment of Size:
0 - No discontinuity of skin/mucous membrane,
1 - 1/4 of previous area & depth of the wound.
3. Assessment of Pain:
0 - No pain,
1 - Localized feeling of pain during movement only but no feeling during rest.
2 - Localized feeling of pain even during rest but not disturbing the sleep.
3 - Localized continuous feeling of pain, radiating & not relieved by rest.
4. Assessment of Tenderness:
0 - Tolerance to pressure,
1 - Little response on sudden pressure.
2 - Wincing effect on super slight touch.
3 - Resists to touch & rigidity.
5. Assessment of Burning:
0 – No burning,
1 - Little, localized & some time feeling of burning sensation.
2 - More localized & often burning sensation which does not disturbed sleep.
3 - Continuous burning sensation with disturbed sleep.
6. Assessment of Itching
0 - No itching,
1 - Slight, Localized itching sensation which is relieved by rest.
2 - More, Localized & often itched but not disturbs sleep.
3 - Continuous itching with disturbed sleep.
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7. Assessment of Color:
0 – Normal pigmentation & of skin,
1 - Slight red.
2 - Reddish black.
3 - Pale yellow! Blackish / Bluish
8. Assessment of Margin & Surface:
0 – Adheres margin,
1 - Smooth, even & regular.
2 - Rough, regular & inflamed.
3 - Rough, irregular & angry look.
9. Assessment of Base/Floor:
0 - Smooth, regular & with healthy granulation tissue,
1 - Smooth, irregular, slight discharge, less granulation tissue, needs dressing & soft scar.
2 - Rough, regular wet with more discharge, needs dressing & having firm scar.
3 - Rough, irregular with profuse discharge, needs frequent dressing & having hard scars.
10. Assessment of Smell:
0 - No smell,
1- Bad smell
2 - Tolerable unpleasant
3 - Foul smell which is intolerable.
11. Assessment of Swelling:
0 – Absent,
1 - Slight red, tender & hot with painful movement & without indurations.
2 - More red, having painful movement, with more local temperature & with indurations.
3 – Angry look, hot, resist to touch & with more indurations.
12. Assessment of Discharge:
0 - No discharge / dry dressing,
1 - Scanty occasional discharge & little wet on dressing.
2 - Often discharge & with blood on dressing
3 - Profuse, continuous discharge which needs frequent dressing.
13. Assessment of unhealthy Granulation Tissue:
0 - Healthy granulation tissue,
1 - Smooth less & irregular granulation base covered with slight discharge.
2 - Little unhealthier granulation tissue & discharge which needs dressing.
3 - Rich unhealthy granulation tissue with profuse discharge, & needs frequent dressing.
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STATISTICAL ANALYSIS:
All information which are based on various parameter was gathered and statistical was carried out in terms
of mean (X), standard deviation (S.D.), standard error (S.E.), paired test (t-value) and finally result were
incorporated in term of probability (p) as – p < 0.05 - Insignificant p < 0.01 - Significant p < 0.001- Highly
Significant.
RESULTS:
Based on the statistical analysis, the effects of the drug on various parameters were studied and following
results were obtained.
Table 1 showing Effect on cardinal sign and symptoms of Group A patients (Karanjadhya Ghrita):
Cardinal
symptoms
Mean
Score (AT)
Percentage
Relief
Size
Mean
Score
(BT)
0.9
0.28
69.23
Pain
1.9
0.76
Tenderness
1.75
Burning
sensation
Swelling
SD
SE
T
P
0.99
0.22
4.04
<0.001
60
1.18
0.26
7.21
<0.001
0.61
65
0.99
0.22
7.87
<0.001
1.5
0.98
35
0.81
0.18
8.32
<0.001
0.35
0.05
84.61
0.57
0.13
2.74
<0.001
1.1
0.35
68.42
0.77
0.17
6.4
<0.001
0.75
0.3
80
0.62
0.14
5.39
<0.001
0.8
0.27
66.66
0.6
0.13
5.96
<0.001
0.45
0.23
50
0.67
0.15
3.01
<0.001
Discharge
1.5
0.47
68.42
0.87
0.19
7.75
<0.001
Unhealthy
granulation
tissue
Itching
0.1
0.04
64.28
0.44
0.09
1.03
>0.05
0.85
0.51
40
0.65
0.15
5.81
<0.001
Color
(Abnormal)
Margin
(Unhealthy)
Base
(Abnormal)
Smell
BT = No of Patients before Treatment, AT = No of Patients after Treatment, SD =Standard Deviation, SE =
Standard Error of Mean, T = Paired T Test, P = Probability Value
Result: In Chronic Wound the size was decreased 69.23% which was statistically highly significant. There
was considerable improvement (> 80 %) in swelling and unhealthy margin. Pain, tenderness, discharge and
unhealthy granulation tissue showed moderate effect (between 60 to 70 percent) in the chronic wounds.
1750
Table 2 showing Effect on cardinal sign and symptoms of Control group (Group B)
Cardinal
symptoms
Size
Mean Score
(BT)
0.87
Mean Score
(AT)
0.33
Pain
1.8
0.76
Tenderness
1.99
Burning
sensation
Swelling
Percentage
Relief
62.31
SD
SE
T
P
0.98
0.12
4.06
<0.001
58
1.23
0.28
6.23
<0.001
0.71
64.1
0.87
0.24
7.87
<0.001
1.09
0.73
33.25
0.86
0.16
7.32
<0.001
0.43
0.08
82.13
0.76
0.11
2.32
<0.001
Color
(Abnormal)
Margin
(Unhealthy)
Base (Abnormal)
1.24
0.42
66.23
0.97
0.19
5.9
<0.001
0.76
0.16
78.6
0.66
0.14
5.12
<0.001
0.87
0.31
64.1
0.58
0.12
6.23
<0.001
Smell
0.45
0.25
45
0.67
0.14
2.99
0.422
Discharge
1.6
0.68
57.8
0.89
0.16
6.57
<0.001
Unhealthy
granulation
tissue
Itching
0.1
0.05
53.2
0.54
0.06
1.01
>0.05
0.82
0.52
36.9
0.67
0.14
5.86
0.641
Table 3: table showing improvement in various types of the wounds
Diagnosis
Lacerated
wound.
Post-Operative
wounds
Fissure
Burn
Pressure sores
Trophic ulcer
Varicose ulcer
Other (wart
etc.)
Cured
Cured Markedly
Patients %
improved
Patients
-
Markedly Improved Improved Unchanged Unchanged
improved Patients
%
Patients
%
%
-
2
66.67
1
33.33
-
-
-
-
8
1
-
72.72
50
-
3
-
27.28
-
1
2
1
50
100
100
1
100
Overall Result of Therapy in Chronic Wound Group: It has been concluded that 72.72% of treated cases
of fissure were cured, followed by 66.67% of cases of post- operative wound were cured. Similarly some
1751
cases (50%) of Pressure sores were also reported cured; all the patients of varicose wound were unchanged
after treatment.
DISCUSSION:
Wound healing is a normal physiological event which outsets immediately after injury till the formation of
healthy scar. Karanjadhya Ghrita contains 16 ingredients including Go-ghrita. “Vrana Ropana” property of
Karanjadhya ghrita is primarily due to Haridra, Nimba, Karanja and Yashthimadhu and “Vrana Shodhana”
property is due to Anantmool, Raktchandan, Patola and Jati. “Vrana Pachan” property is mainly attributed to
Haridra, Patola, Ushira and Krishana Sariva14. Go-ghrita is used in most ayurvedic formulations. Cow
ghee’s regenerative properties are also useful for healing wounds and promoting the growth of healthy cells.
This wound healing ability has also been clinically proven15, 16.
Mode of Action: The mode of action (karmukta) of Karanjadhya Ghrita along with its properties is given
below –
Table showing the properties of Karanjadhya Ghrita
S.No. Ingredients
Karmukta
Karanj and other
Vedanasthapaka, Sothahara, Dipana, Dahahara, Vrana Shodhaka,
1
drugs
vrana ropaka.
Putihara, Krimighna, Kandughna, Shoolhara and Ama
Pachaka.
Go ghrita
Agnidipaka, Varnya, Dhatuvardhaka, Shothahara,
2
Jivaniya, Balya, Vishaghna,Vrana Ropaka, Prasadak,
Dhatuvardhaka, Prinana and Vranya.
In order to achieve the main goal of wound healing, it is necessary to remove maximum debridement at the
site of Vrana (Vrana Shodhan). By virtue of Lekhana Putihara, Dahahara, Kandughana and Vrana Ropana
properties of Karanjadhya Ghrita contents, the sthaniya Dhatu Dushti is ceased. The second step in the path
of healing is Vrana Pachan and is due to Ama Pachaka properties of the contents. The third step of healing is
to enhance (Vrana Ropan) and for this purpose, Go-ghrita is made easy way. Lipophilic action of ghee easily
facilitate transportation to a target organ and final delivery, inside the cell, because cell membrane also
contains lipid. This lipophilic nature of ghee facilitates entry of the formulation into the cell and its delivery
to the mitochondria, microsome and nuclear membrane .According to Ayurveda, Go-ghrita is beneficial for
Rasa Dhatu and Rakta Dhatu. It possess Sheta Guna as well. With all these properties, Ghrita enhance the
Rasagni and Raktagini and thus increase the Ropana Karma17.
CONCLUSION:
Observation, analysis and results of the present study can be concluded as follow –
 Topical application of Karajadhya Ghrita is found efficacious in the wound healing. It possesses
sufficient efficacy in “Vrana Shodan and Ropana” without producing any adverse effects.
 Analysis of types of wounds showed that 72.72% of treated cases of fissure were cured, followed by
66.67% of cases of post- operative wound were cured. Similarly some cases (50%) of Pressure sores
were also reported cured.
 The size was decreased 69.23% which was statistically highly significant.
 There was considerable improvement (> 80 %) in swelling and unhealthy margin.
 Pain, tenderness, discharge and unhealthy granulation tissue showed moderate effect (between 60 to
70 percent) in the chronic wounds.
Hence, from the present study, it can be speculated that Karanjadhya Ghrita possess sufficient efficacy in
Vrana Shodhan and Vrana Ropana without producing any adverse effects.
1752
ACKNOWLEDGEMENT: I acknowledge National Institute of Ayurveda for allowing me to work and
perform this study. I thank my Guide Dr V Nageshwar Rao and HOD – Dr Laxmikant Dwivedi for
providing me directions for preparing the Ghrita and guiding me for the clinical study.
Conflicts of interest: None declared.
Source of support: Nil
References:
1. Sushruta
“Sushrut
Samhita”
(1988)
Commentory
by
Dalhanacharya
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sangraha.Chaukhambha Orientalia Varanasi (2002) Sutra sthaana, Chapter-19.
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Chaukhamba Subharti Prakashan, Varanasi, reprinted, Charak Chikitsa, 25/24, 25, 83; 2003.p.84041, 853.
3. Shastri Sudarshan Madhav Nidana madhukosha-vidyotini, Hindi commentary, Uttaradha,
Chukhamba Sanskrit Sansthan, Varanasi, Ch 42 (sharir vrana nidana); p 102.
4. Vadiyaksabdasindhu, by Umesh Chandra Gupta, Chaukhamba Orientalia, Varanasi, 5th edition;
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5. Sushruta, Sushruta Samhita, Ayurveda Tatwa Sandeepika Hindi Commentry, edited by Kaviraj
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8. Williams JZ, Barbul A (2003) Nutrition and wound healing. Surg Clin North Am 83: 571-596.
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42/ 81-83, published by Swami Jayaramdas Ramprakash Trust, Jaipur, Edition 1, 1993.
12. Sushruta Samhita Chikitsa Sthana, Ayurveda Tatwa Sandeepika Hindi Commentry, edited by
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Reprint 2009.
13. Bhaisajya Ratnavali, Govinddas, Hindi Commentry by Ambika Dutt Shastri, Chapter Jwaradhikara
5/1285, Chaukhamba Sanskrit Sansthan, Varanasi, Edition 15th 2002.
14. Prasad V, Dorle AK (2006) Evaluation of ghee based formulation for wound healing. J
Ethnopharmacol 107: 38-47.
15. Demling RH (2000) Oxandrolone, an anabolic steroid, enhances the healing of a cutaneous wound in
the rat. Adv Skin Wound Care 8: 97-102.
16. Database of Medicinal plants; Vol II, p 292; Vol III p 332, 561; Vol I p 152, 289, 394; Vol V, p 269.
17. Hiremath SG. A Text Book of Bhaishajya Kalpana. 2nd revised ed. Part 1, Sneha Kalpana, Chapter
22, Varanasi: Chaukhamba Orientalia, 2005; 243.
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A Randomized Controlled Clinical Study of Karanjadhya Ghrita in

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