Presentation of the portable luminometer
Transcription
Presentation of the portable luminometer
Portable device for personal ecology and medicine Ivan A Denisov Lead engineer of MOLPIT +7 (960) 769-07-25 denisov@molpit.com The problem A growing need in bioassay technologies for: ● ● ● rapid detection of pollutants in water; detection of poor quality products; providing schools and universities with modern lab activities. Limitations of the current bioassay methods: ● chemical analysis doesn’t show toxicity effects on biological system. ● living organisms tests are expensive, give high measurement error and hard to use; ● the cost of sensitive enough luminometers is too high; ● the lack of affordable for consumer diagnostic devices. 2 Problem solving Compact diagnostic device Special Component Software Enzyme biomodule Modern solid-state detectors Microelectronics for digital processing of signals 3 Solution: enzyme biotests Bioluminescent Enzyme System Technology (BEST) allows to carry out express bioassays of water and physiological fluid for pollutants Advantages of bioluminescent test: ● Broad screening – 25,000 toxins ● Rapid/emergency screening adaptability ● Focused toxin-class identification systems ● ● The method is compatible with standard laboratory equipment Shelf-life of reagents up to one year 4 Solution: LumiShot luminometer Portable luminometer for high school and university practical course. This cuvettebased device is suitable for routine bioassay in bioluminescent research. metal cover сuvette sample compartment detection module сuvettes body cover hinge system electronics units device body miniUSB-port hole for fixation cuvettes and device support 5 Market segments education and research environmental monitoring bioassay of food products 6 The market Luciferase enzymes and other enzymes $100M. Chemical and biological agent detection $640M Schools Middle class families USA Europe Revenue Forecasts Asia Russia ~53 000 ~5,4 M Analytical devices market * Frost & Sullivan, 2010 7 Sensitivity, moles of luciferase Comparison with competitors 1E-21 Promega GloMax 20/20 1E-18 1E-15 Nauka BLM 3606m1 Berthold Tristar Nauka BLM 8802mpt LumiShot Berthold Lumat LB 9507 Berthold Junior LB 9509 1E-12 Higiena SystemSure III MSU Lum1 1E-9 Biotox-10M $850 $2,000 $3,500 $5,000 Cost $10,000 $100,000 8 The product distribution plan Internet-shop LumiShot Distributor of school equipment Reagents Schools and universities 9 R&D pipeline April-May Trial sales and presales June-July Bug fixes & development of production roadmap August-September Start of mass production October-December Sales & support LumiShot 10 The team Elena N. Esimbekova h.i.=8, publications 50+ Senior researcher Prof. Valentina A. Kratasyuk, Dr.Sc., h.i.=10, publications 200+ Scientific director Nadezhda V. Rimazkaya Oleg S. Sutormin Laboratory engineer Laboratory engineer Galina V. Zhukova Laboratory engineer Albert M. Oyun CEO Viktoria I. Lonshakova-Mukina Researcher Prof. Peter I. Belobrov Dr.Sc., h.i.=7, publications 120+ Scientific consultant Kirill A. Lukyanenko Researcher Ivan A. Denisov h.i.=1, publications 7 Lead and software engineer Anton S. Yakimov Design engineer Vladimir V. Sorokin Electronics engineer 11 Intellectual property Patents: Know-How: 1. Method of ''Enzimolyum'' fabrication 1. Unique collection of luminous bacteria 2. Water pollution bioassay 2. Extensive database of bioassays 3. Portable luminometer 3. Proprietary manufacturing process Specifications: 1. Preparation of ''Enzimolyum'' #003534 4. Original software 5. Original signal amplifier Trade Mark: 1. Trade mark Enzimolyum™ 12 Sales plan LumiShot ($850) schools, universities, research laboratories Сustomers Sales Profit 2015 $25k $10k 2016–2017 $420k $170k 2018–2020 $1.5m $0.5m 13 Requested funding Start of LumiShot mass production – 60 000 $ Third party services (5%) Production (50%) Expendable materials (5%) Wages fund (40%) 14