CV - Research - The University of Texas at Austin

JEANNE C. STACHOWIAK, PH.D.
Assistant Professor
THE UNIVERSITY OF TEXAS AT AUSTIN
Department of Biomedical Engineering
Institute for Cellular and Molecular Biology
The University of Texas at Austin
jcstach@austin.utexas.edu
http://research.bme.utexas.edu/stachowiak/index.html
RESEARCH OBJECTIVES
The vision of my lab is to reverse engineer lipid membrane function as a route to understanding cellular physiology
and constructing clinically translatable materials and systems. The health of cells, tissues, and organisms relies on
communication of biochemical instructions across membrane interfaces. Perturbation of membrane structure and
organization play a role in diverse diseases. Through quantitative molecular-scale measurements and the design of
biomimetic materials, research in our laboratory aims to understand the physical basis of cellular membrane organization
and develop systems that engage in transmembrane communication with cells.
EDUCATION
THE UNIVERSITY OF CALIFORNIA, BERKELEY
Doctor of Philosophy, Mechanical Engineering, May 2008
Advisor: Professor Daniel A. Fletcher
THE UNIVERSITY OF CALIFORNIA, BERKELEY
Master or Science, Mechanical Engineering, May 2004
Advisor: Professor Arun Majumdar
THE UNIVERSITY OF TEXAS AT AUSTIN
Bachelor of Science, Mechanical Engineering, Highest Honors, May 2002
APPOINTMENTS
01/12 – present
ASSISTANT PROFESSOR, DEPARTMENT OF BIOMEDICAL ENGINEERING
INSTITUTE FOR CELLULAR AND MOLECULAR BIOLOGY
THE UNIVERSITY OF TEXAS AT AUSTIN
Principle investigator and head of an independent laboratory. Responsible for developing and
directing a program of original research in biomaterials and biophysics. Responsible for
mentoring graduate students, postdoctoral appointees, and undergraduate researchers; teaching at
the graduate and undergraduate levels; serving the scientific community at the local, national, and
international levels.
08/08 – 12/11
SENIOR MEMBER OF THE TECHNICAL STAFF
SANDIA NATIONAL LABORATORIES, LIVERMORE, CA
As an independent researcher, reconstructed lipid membranes from component parts and applied
advanced imaging tools and physical principles to observe and interpret their behavior. Led work
that revealed a new mechanism for lipid membrane bending that relies on spatial confinement of
protein binding to membranes. Initiated internal and external collaborations with biologists,
chemists, engineers, and theorists. Supervised students and postdoctoral researchers. Developed
successful original research proposals.
08/05 - 08/08
GRADUATE STUDENT RESEARCHER, Supervised by Professor Daniel Fletcher
DEPARTMENT OF BIOENGINEERING, UNIVERSITY OF CALIFORNIA, BERKELEY
Developed microfluidic systems for delivering drugs and mimicking cellular encapsulation in
lipid membranes.
Jeanne C. Stachowiak, Ph.D.
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Designed a piezo-electric driven microfluidic jet injector device capable of needle-free
transdermal drug delivery. Optimized injector performance using fluid-dynamic modeling
and performed in vitro and in vivo testing to demonstrate transdermal delivery.
Developed a microfluidic method for precisely encapsulating biochemical solutions within
lipid vesicles. Demonstrated universal solute encapsulation in monodisperse, unilamellar
vesicles formed by this method, creating new opportunities for biochemical reconstitution
and bio-inspired drug delivery.
07/04 - 09/05
MEMBER OF THE TECHNICAL STAFF
SANDIA NATIONAL LABORATORIES, LIVERMORE, CA
Designed and tested an autonomous microfluidic system capable of preparing bacterial cells and
spores collected from air for identification by capillary gel electrophoresis.
08/02 – 07/04
GRADUATE STUDENT RESEARCHER, Supervised by Professor Arun Majumdar
DEPARTMENT OF MECHANICAL ENGINEERING, UNIVERSITY OF CALIFORNIA, BERKELEY
Fabricated an array of micro-cantilever sensors using a multi-mask clean-room process and
chemical surface functionalization strategies.
Used sensor array to investigate DNA
hybridization mechanics and detect protein-protein interactions relevant to early cancer detection.
Summers 2001-2002
RESEARCH INTERN, SANDIA NATIONAL LABORATORIES, ALBUQUERQUE, NM
Determined the mechanical properties of explosive materials through ultrasonic testing.
01/01 - 05/02
UNDERGRADUATE RESEARCHER, Supervised by Professor David Bourell
DEPARTMENT OF MECHANICAL ENGINEERING, UNIVERSITY OF TEXAS AT AUSTIN
Investigated solid state interdiffusion of copper and silver for applications in rail-gun design
using computational and experimental results.
Summer 2000
STUDENT INTERN, FORD MOTOR COMPANY, STEERING SYSTEMS DIVISION, DEARBORN, MI
Designed and implemented a PC-based calibration system for Ford's electronically variable
hydraulic power assisted rack and pinion steering system.
REFEREED JOURNAL PUBLICATIONS
1. N. Momin, S. Lee, A. K. Gadok, D. J. Busch, G. D. Bachand, C. C. Hayden, J. C. Stachowiak, D. Y. Sasaki
"Designing Lipids for Selective Partitioning Into Liquid Ordered Membrane Domains" 2015, RSC Soft Matter, 11,
3241-3250.
2. C. W. Coyne, K. Patel, J. Heureaux, J. C. Stachowiak, D. A. Fletcher, A. P. Liu, “Lipid Bilayer Vesicle Generation
Using Microfluidic Jetting” 2014, Journal of Visualized Experiments, DOI: 10.3791/51510
3. J. C. Stachowiak, F. Brodsky, E. Miller “A Cost-Benefit Analysis of the Physical Mechanisms of Membrane
Curvature” 2013, Nature Cell Biology, 15, 1019-1027.
4. D. Y. Sasaki, N. Zawada, S. F. Gilmore, P. Narasimmaraj, M. A. Sanchez, J. C. Stachowiak, C. C. Hayden, H. L.
Wang, A. N. Parikh, A. P. Shreve, “Lipid Membrane Domains for the Selective Adsorption and Surface Patterning of
Conjugated Polyelectrolytes,” 2013, Langmuir, 29, 5214-5221.
5. C. S. Scheve, P. Gonzales, N. Momin, J. C. Stachowiak, “Protein-protein crowding resists membrane phase
separation,” 2013, Journal of the American Chemical Society, 135, 1185-1188.
6. J. C. Stachowiak*, E. M. Schmid, C. J. Ryan, H. S. Ann, D. Y. Sasaki, M. B. Sherman, P. L. Geissler, D. A. Fletcher,
C. C. Hayden, “Membrane bending by protein-protein crowding,” 2012, Nature Cell Biology, 9, 944-949.
*Corresponding author, Highlighted by Faculty of 1000, Nature Cell Biology News and Views, and Nature
Structural and Molecular Biology Research Highlights.
7. D. L. Richmond, E. M. Schmid, S. Martens, J. C. Stachowiak, N. Liska, D. A. Fletcher, “Forming giant vesicles with
controlled membrane composition, asymmetry, and contents,” 2011, PNAS, 108, 9431-9436.
8. F. J. Zendejas, R. J. Meagher, J. C. Stachowiak, C. C. Hayden, D. Y. Sasaki, “Orienting lipid domains in giant
vesicles using an electric field,” 2011, Chemical Communications, 47, 7320-7322.
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Jeanne C. Stachowiak, Ph.D.
9. J. C. Stachowiak, C. C. Hayden, D. Y. Sasaki, “Targeting proteins to liquid ordered domains in lipid membranes,”
2011, Langmuir, 27, 1457-1462.
10. J. C. Stachowiak*, C. C. Hayden, D. Y. Sasaki, “Steric confinement of proteins on lipid membranes can drive
curvature and tubulation,” 2010, PNAS, 107, 7781-7786. *Corresponding author
11. T. H. Li, J. C. Stachowiak, D. A. Fletcher, “Mixing Solutions in Inkjet Formed Vesicles,” 2009, Methods in
Enzymology, 465, 75-94.
12. J. C. Stachowiak, D. L. Richmond, T. H. Li, F. Brochard-Wyart, D. A. Fletcher, “Inkjet Formation of Unilamellar
Lipid Vesicles for Cell-like Encapsulation,” 2009, Lab on a Chip, 9, 2003-2009. Cover article, highlighted in
Chemical Biology
13. J. C. Stachowiak, T. H. Li, A. A. Arora, S. Mitragotri, D. A. Fletcher, “A Needle-free Jet Injector for Dynamically
Controlled Transdermal Drug Delivery,” 2009, Journal of Controlled Release, 135, 104-112.
Cover article,
highlighted by editor
14. J. C. Stachowiak, D. L. Richmond, T. H. Li, A. P. Liu, S. H. Parekh, D. A. Fletcher, “Unilamellar Vesicle Formation
and Encapsulation by Microfluidic Jetting,” 2008, PNAS, 105, 4697-4702. Highlighted in Lab on a Chip
15. M. Yue, J. C. Stachowiak, H. Lin, R. Datar, R. Cote, A. Majumdar, “Label-free Protein Recognition 2D Array Using
Nanomechanical Sensors,” 2008, Nano Letters, 8, 520-524.
16. J. C. Stachowiak, M. G. von Muhlen, T. H. Li, L. Jalilian, S. H. Parekh, D. A. Fletcher, “Piezoelectric Control of
Needle-Free Transdermal Drug Delivery,” 2007, Journal of Controlled Release, 124, 88-97.
17. J. C. Stachowiak, et al., “Autonomous Microfluidic Sample Preparation System for Protein Profile-Based Detection of
Aerosolized Bacterial Cells and Spores,” 2007, Analytical Chemistry, 79, 5763-5770.
18. J. C. Stachowiak, M. Yue, K. Castelino, A. Chakraborty, A. Majumdar, “Chemomechanics of Surface Stresses
Induced by DNA Hybridization,” 2006, Langmuir, 22, 263-268.
19. M. Yue, J. C. Stachowiak, A. Majumdar, “A 2-D microcantilever array for multiplexed biomolecular analysis,” 2004,
Mechanics and Chemistry of Biosystems, 1, 211-220.
HONORS
2014
2011
2008
2005
2005
2002
2002
2002
2002
1998
National Science Foundation CAREER Award
Cornell University Distinguished Honor for Faculty Mentoring of Co-op Students
Soroptimist Founder Region Dissertation Fellowship Award
Sandia National Laboratories, Award for Individual Employee Excellence
Sandia National Laboratories, Team Excellence Recognition Award
National Science Foundation Graduate Research Fellow
ARCS Foundation Graduate Research Fellow
US Department of Defense Graduate Research Fellowship Award
Bachelors of Science in Mechanical Engineering with highest honors (Ranked 1st in class)
Rockwall High School Valedictorian
CONFERENCE PRESENTATIONS
1. J. C. Stachowiak, “Membrane bending by protein-protein crowding,” Annual Meeting of the American Physical
Society, March 2014, Invited presentation.
2. A. K. Gadok, J. C. Stachowiak, “Incorporating Intercellular Junction Proteins in Membrane Vesicles to Enable
Biochemical Communication with Cells,” Annual Meeting of the Biomedical Engineering Society, September 2013.
3. C. S. Scheve, J. C. Stachowiak, “Steric pressure between proteins opposes membrane phase separation,” Annual
meeting of the Biophysical Society, February 2013.
4. J. C. Stachowiak, “Biomechanics of membrane bending,” Southwestern Biomedical Engineering Conference, May
2012.
5. J. C. Stachowiak, E. M. Schmid, C. J. Ryan, H. S. Ann, D. Y. Sasaki, P. L. Geissler, D. A. Fletcher, C. C. Hayden,
“Protein-protein crowding as a driving force for membrane bending during clathrin-mediated endocytosis,”
Biophysical Society Annual Meeting, February 2012.
6. J. C. Stachowiak, E. M. Schmid, C. J. Ryan, H. S. Ann, D. Y. Sasaki, P. L. Geissler, D. A. Fletcher, C. C. Hayden,
“Epsin1 bends membranes by molecular crowding,” Gordon Research Conference on Molecular Membrane Biology,
July 2011.
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Jeanne C. Stachowiak, Ph.D.
7. J. C. Stachowiak, C. C. Hayden, M. A. A. Sanchez, J. Wang, D. Y. Sasaki, “Selective protein affinity and structure
transformation of domains in lipid membranes,” American Chemical Society National Meeting, March 2011.
8. E. M. Schmid, D. L. Richmond, J. C. Stachowiak, D. A. Fletcher, “Bending membranes with proteins: Lessons from
cellular reconstitution,” American Chemical Society National Meeting, March 2011.
9. F. J. Zendejas, R. Meagher, J. C. Stachowiak, C. C. Hayden, J. Wang, D. Y. Sasaki, “Orientation of lipid domains in
giant vesicles by electric field,” American Chemical Society National Meeting, March 2011.
10. J. Stachowiak, E. M. Schmid, M. B. Sherman, D. A. Fletcher, C. C. Hayden, D. Y. Sasaki, “Confinement of protein
binding to lipid domains as a tool for directed assembly of 3-D architectures,” ACS Pacifichem, December 2010.
11. C. C. Hayden, J. C. Stachowiak, J. S. Hwang, E. A. Abate, M. S. Kent; D. Y. Sasaki, “Directed formation of lipid
micro-domains functioning as high affinity His-tagged protein binding sites,” ACS Pacifichem, December 2010.
12. D. Y. Sasaki, H. Liu, A. Carroll-Portillo, G. D. Bachand, C. C. Hayden, J. C. Stachowiak, “Inverted microtubulekinesin activity in the formation of lipid nanotubular structures,” ACS Pacifichem, December 2010.
13. J. C. Stachowiak, C. C. Hayden, D. Y. Sasaki, “Recognition-driven Actuation of Lipid Domains, Nano-tubules, and
Self-assembled Networks,” Materials Research Society National Meeting, April 2010.
14. J. C. Stachowiak, C. C. Hayden, D. Y. Sasaki, “Formation of Lipid Membrane Domains and Tubules Using
Reversible Metal and Protein Affinity,” American Chemical Society National Meeting, San Francisco, March 2010.
15. D. Y. Sasaki, H. Liu, A. Carroll-Portillo, G. D. Bachand, C. C. Hayden, J. C. Stachowiak, “Formation of Lipid
Nanotubular Networks Via Surface Affinity and Pulling of Giant Vesicles with Gliding Microtubules,” American
Chemical Society National Meeting, San Francisco, March 2010.
16. J. C. Stachowiak, C. C. Hayden, D. Y. Sasaki, “Steric Confinement of Proteins in Lipid Domains Can Drive
Membrane Curvature and Tubulation,” Biophysical Society Annual Meeting, San Francisco, February 2010.
17. D. L. Richmond, E. M. Schmid, S. Martens, J. C. Stachowiak, N. Liska, D. A. Fletcher, “Engineering Vesicle
Membranes for Cellular Reconstitutions,” Biophysical Society Annual Meeting, San Francisco, February 2010.
18. J. C. Stachowiak, T. H. Li, D. L. Richmond, D. A. Fletcher, “Microfluidic Assembly of Cell-like Systems in Giant
Unilamellar Lipid Vesicles,” MicroTAS Annual Meeting, San Diego, October 2008.
19. J. C. Stachowiak, T. H. Li, D. L. Richmond, A.P. Liu, S. H. Parekh, D. A. Fletcher, “High Reynolds Number
Microfluidics for Drug Delivery,” MicroTAS Annual Meeting, Paris, October 2007.
20. J. C. Stachowiak, T. H. Li, D. L. Richmond, A.P. Liu, S. H. Parekh, D. A. Fletcher, “Encapsulation of Solutes in
Giant Lipid Vesicles by Microfluidic Jetting,” Biomedical Engineering Society Annual Meeting, Los Angeles,
October 2007.
21. J. C. Stachowiak, T. H. Li, J. Rasooly, W. A. Lam, D. A. Fletcher, “Development of a Piezoelectric Microjet Injector
for Transdermal Drug Delivery,” MicroTAS Annual Meeting. Tokyo, November 2006.
22. J. C. Stachowiak, J. Rasooly, D. A. Fletcher, “Design of a Piezoelectric Microjet for Needleless Drug Delivery,”
ASME Summer Bioengineering Conference, Amelia Island, June 2006.
23. J.C. Stachowiak, E.E. Fischer, P. Caton, et. al., “Automated sample preparation system for rapid biological threat
detection,” ASME Annual Meeting, Orlando, November, 2005.
24. M. Yue, J. C. Stachowiak, A. Chakraborty, A. Majumdar, “Nanomechanical Sensor Array for Detection of
Biomolecular Bindings,” ASME Integrated Nanosystems Conference, Pasadena, September, 2004.
INVITED PRESENTATIONS
Annual Meeting of the Biophysical Society of the USA, Baltimore, Maryland, February, 2015
Annual Meeting of the Biophysical Society of Japan, Invited Speaker, September 2014
Duke University, Departments of Cell Biology and Molecular, Genetics and Microbiology, Seminar Series, Spring 2014
Lakeway Men’s Breakfast Club, Austin, TX, Speaker Series, Spring 2014
University of Southern Illinois, Departments of Chemistry and Biochemistry, Seminar Series, Spring 2014
University of Texas at Austin, Center for Systems and Synthetic Biology, Seminar Series, Fall 2013
University of Texas at Austin, Institute for Cellular and Molecular Biology, Seminar Series, Fall 2012
University of Texas Health Sciences Center, San Antonio, Department of Biochemistry, Seminar Series, Spring 2012
University of Texas at Austin, Department of Physics, Center for Nonlinear Dynamics, Seminar Series, Spring 2012
University of California, Berkeley, Department of Bioengineering, Seminar Series, Fall 2011
University of Pennsylvania, Department of Bioengineering, Seminar Series, Spring 2011
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Jeanne C. Stachowiak, Ph.D.
Lawrence Berkeley Laboratory, Physical Biosciences, Seminar Series, Spring 2011
Georgia Institute of Technology, Department of Mechanical Engineering, Seminar Series, Spring 2011.
Boston University, Department of Bioengineering, Seminar Series, Spring 2011.
University of Illinois Urbana-Champaign, Department of Mechanical Engineering, Seminar Series, Spring 2011
Tufts University, Department of Chemical and Biological Engineering, Seminar Series, Spring 2011
University of Maryland, Departments of Bioengineering and Chemical Engineering, Seminar Series, Spring 2011
Institute for Bioscience and Biotechnology at the University of Maryland, Seminar Series, Spring 2011
University of Texas at Austin, Department of Biomedical Engineering, Seminar Series, Spring 2011
“Bio-based Future” workshop at UC Berkeley Synthetic Biology Institute, 2011
Research Presentation for Dr. Eric Moore, Division Chief, Basic and Supporting Science Division, DTRA, 2010
Lawrence Berkeley National Laboratories, Dr. Cynthia McMurray’s Laboratory, 2010
Research Presentation for Albert Romig, Chief Operations Officer, Sandia National Laboratories, 2010
Sandia National Laboratories Bioscience External Advisory Panel Symposium, 2009
Stanford University, Professor Nicholas Melosh’s and Steven Boxer’s Laboratories, 2008
Palo Alto Research Center, Palo Alto, CA, Research Seminar, 2008
SRI International, Menlo Park, CA, Research Seminar, 2008
University of California, San Francisco, NIH Nano Medicine Center Symposium, PI: Wendell Lim, 2007
University of California, Berkeley, Professor Susan Muller’s Laboratory, 2006
University of California, Berkeley Sensor and Actuator Center, Seminar Series, 2006
PATENTS
J. C. Stachowiak, D. A. Fletcher, T. H. Li, S. Parekh, A. P. Liu, D. L. Richmond, E. Schmid, “Forming an Artificial Cell
with Controlled Membrane Composition, Asymmetry, and Contents,” US Provisional Patent Pending.
TEACHING EXPERIENCE
Fall 2013
Spring 2013
Fall 2012
2010
Course Instructor, BME 314: Engineering Foundation of BME, The University of Texas at Austin.
Course Instructor, BME 314: Engineering Foundation of BME, The University of Texas at Austin.
Course Instructor, BME 382J: Molecular Biophysics, The University of Texas at Austin.
Teaching Assistant, Cell Physiology Course, Marine Biological Laboratory, Woods Hole, MA.
STUDENTS AND RESEARCHERS MENTORED
Dr. David Busch, Postdoctoral Scholar, The University of Texas at Austin (current)
Dr. Silvia Ferrati, Postdoctoral Scholar, The University of Texas at Austin (current, with Dr. Hugh Smyth)
Mr. Ryan Woodall, Graduate Student Researcher, The University of Texas at Austin (current)
Ms. Chi (Aimee) Zhao, Graduate Student Researcher, The University of Texas at Austin (current)
Mr. Zachary Imam, Graduate Student Researcher, The University of Texas at Austin (current)
Mr. Wilton Snead, Graduate Student Researcher, The University of Texas at Austin (current)
Ms. Avinash Gadok, Graduate Student Researcher, The University of Texas at Austin (current)
Mr. Brian Li, Undergraduate Student Researcher, The University of Texas at Austin (current)
Mr. Saad Jafri, Undergraduate Student Researcher, The University of Texas at Austin (current)
Mr. Connor Vershel, Undergraduate Student Researcher, The University of Texas at Austin (current)
Ms. Tu Cao, Undergraduate Student Researcher, The University of Texas at Austin (current)
Ms. Laura Kenyon, Undergraduate Student Researcher, The University of Texas at Austin (current)
Mr. Stefan Bordovsky, Undergraduate Student Researcher, The University of Texas at Austin (current)
Mr. Aaron Alpar, Undergraduate Student Researcher, The University of Texas at Austin (current)
Mr. Hisham Ali, Undergraduate Student Researcher, The University of Texas at Austin (current)
Ms. Christine Smid, Graduate Student Researcher, The University of Texas at Austin, M.S. (2012-2013)
Ms. Katherine Ha, Undergraduate Student Researcher, The University of Texas at Austin (2014)
Ms. Mansi Raythatha, Undergraduate Student Researcher, The University of Texas at Austin (2013-2014)
Mr. Isac Lee, Undergraduate Student Researcher, The University of Texas at Austin (2012-2014)
Ms. Noor Momin, Undergraduate Student Researcher, The University of Texas at Austin (2012-2015)
Mr. Justin Hauser, Undergraduate Student Researcher, The University of Texas at Austin (2012-2015)
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Jeanne C. Stachowiak, Ph.D.
Ms. Jerin Jose, Undergraduate Student Researcher, The University of Texas at Austin (2012-2013)
Mr. Jacob Sacks, Undergraduate Student Researcher, The University of Texas at Austin (2012-2013)
Mr. Ansel George, Undergraduate Student Researcher, The University of Texas at Austin (2012-2013)
Mr. Paul Gonzales, Undergraduate Student Researcher, The University of Texas at Austin (2012-2013)
Mr. Daniel Nilson, Undergraduate Summer Intern for The University of California, Davis (2011)
Dr. Frank Zendejas, Sandia Postdoctoral Appointee (2010-2011)
Dr. Dianna Maar, Sandia Postdoctoral Appointee (2010-2011)
Ms. Julia Wang, Undergraduate Co-op Student from Cornell University (2010)
Ms. Prihatha Narasimmaraj, Undergraduate Summer Intern from Princeton University (2010)
Ms. Mari Sanchez, Undergraduate Summer Intern from the University of California, San Diego (2010)
Mr. William Shaffenburg, Undergraduate Summer Intern (2009)
Mr. Gerard Whelan, Undergraduate Intern (2008-2009)
Mr. Thomas Li, Undergraduate and Masters Student Researcher, University of California, Berkeley (2006-2009)
Ms. Julia Rasooly, Undergraduate Student Researcher, University of California, Berkeley (2005-2006)
PROFESSIONAL MEMBERSHIP AND SERVICE
Presentation on Biomedical Engineering Careers at LASA High School, Austin, TX, Spring 2014
Faculty Presenter at WE@UT summer camp for high school women as prospective STEM undergraduates, Summer 2013
Faculty Speaker at UT Biomedical Engineering Graduate Women’s Organization Meeting, Summer 2013
Faculty judge for UT BME Undergraduate Research Poster Competition, Spring 2012, 2013
Faculty host for visit of prospective minority students from UT Pan American, Spring 2013
Laboratory exhibitor at “Explore UT” community outreach event, Spring 2013
Biomedical Engineering Society, Member and Session Chair at the 2012 Annual Meeting in Atlanta, GA
Biophysical Society, Member and Session Chair at the 2009 Annual Meeting in San Francisco, and 2013 Annual Meeting
in Philadelphia.
American Chemical Society, Member
Materials Research Society, Member
University of Texas at Austin MITE Summer Program for prospective minority engineering students
Sandia Women’s Committee, Speaker at annual event recognizing math and science achievement of high school women
Berkeley Engineering Techbridge alumni mentor for local high school students
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