BT-W_Anti-Irritant for Objective Irritation - in

Natural Anti-Irritant
Product Name:
Bio-Relaxer
Bio-Tranquilizer
Natural Anti-Irritant Catalogue
1
B103, WOOLIMLIONS VALLEY 2nd B-LINE, SANGDAEWON-DONG, JUNGWON-GU, SEONGNAM,
GYENGGI-DO, SOUTH KOREA, 462-120
Tel:+82 2 573 8575
Email: szhen@ibst.kr
Merits of Natural Anti-Irritant
Natural Anti-Irritant
1. Objective irritation like rash and eczema
Bio-tranquilizer reduces objective irritation
Bio-tranquilizer is subdivided into (BT-W)water part and (BT-O)oil part
a. Multifunctional effects
Anti-inflammation
Anti-oxidation
2. Helps restore skin for better healthier looking
3. Is composed of Natural material/Ingredient
2
Core Technologies
•
Natural Herb Extraction (Specific Activity)
- Natural UV Protector
- Acne, Dandruff, Tinea Pedis
- Extraction of active ingredients from plants and the increased activity by bioconversion
•
Stable Formulation of Active Ingredients
- Stabilized active ingredients through electro-spinning, micro-fluidization,
3D conformational structure and etc
- Extracellular Matrix composition
- Vitamin C & proteases & etc.. were stabilized through BST’ s safety and stability
technology
3
Core Technologies
• Bioconversion
- BCX-A (Anti-acnes) was developed through bio-conversion of Chamaecyparis obtusa’s
leaf extract.
- Whitening agent (arbutin modifier) was developed through symbitic fermentation.
•
Cell culture
- ADSC (Adipose Derived Stem Cell) Filtrate, which contains many
different kinds of active human growth hormone such as EGF, HGF, basic FGF, VEGF
and etc.
- We named ADSC (Adipose Derived Stem Cell) Filtrate as “SMGF” (Stabilized Mega
Growth Factor)
• Human protein cloning
EGF was cloned to vector and isolated from Sacchromyces cerevisae
4
Contents
What is Natural Anti-Irritant?
What does Natural Anti-Irritant do?
How well does Natural Anti-Irritant perform?
5
What is Natural Anti-Irritation?
Bio-Tranquilier
6
What is Natural Anti-Irritant
• The use of functional cosmetics has been
increased substantially, and at the same time,
its side effect (irritation) is also growing
problems for functional cosmetics users.
• Stimulus can be evaluated as two types
– Subjective irritation
– Objective irritation
7
What is Bio-Tranquilizer?
8
What is Bio-Tranquilizer?
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
There are two types of Bio-Tranquilizer making it possible for the
manufacturers to pick the products depending on the manufacturers’ need.
Bio-Tranquilizer
For the application in water phase
Bio-Tranquilizer
For the application in oil phase
9
What is Bio-Tranquilizer?
Potato
Cordyceps
Centella asiatica
Ashitaba
Magnolia
Indicated Substance : Asiaticosides
18-beta glycyrrhetinic acid
Indicated Substance :
BT-W(cordycepin)
10
What is Bio-Tranquilizer?
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
Common name
INCI NAME
Scientific Name
Potato
Solanum tuberosum (potato) extract
Solanum tuberosum
The potato contains vitamins and minerals, as well as an assortment of
phytochemicals, such as carotenoids and natural phenols. The potato is
best known for its carbohydrate content (approximately 26 grams in a
medium potato). The predominant form of this carbohydrate is starch. It
offers protection against colon cancer, improves glucose tolerance and
insulin sensitivity, lowers plasma cholesterol and triglyceride
concentrations,
Anti-cancer, anti-aging, Lower triglyceride
11
What is
What
Bio-Tranquilizer?
is Bio-Relaxer
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
Common name
INCI NAME
Scientific Name
Cordyceps
CORDYCEPS MILITARIS MYCELIUM E
XTRACT
CORDYCEPS MILITARIS
Cordyceps is a genus of ascomycete fungi that includes about 400
identified species and many yet to be described. All Cordyceps species are
endosparasitoids, parasitic mainly on insects and other arthropods; a few
are parasitic on other fungi. The Cordyceps have a long history as
medicinal fungi.Some polysaccharide components and cordycepin have
some anticancer activity in preliminary in vitro and animal studies. It has
been used to protect the bone marrow and digestive systems of mice from
whole body irradiation
Anti-cancer, Hypoglycemic efficacy
12
What is Bio-Tranquilizer?
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
Common name
INCI NAME
Scientific Name
Ashitaba
Angelica keiskei leaf/stem extract
Angelica keiskei
Angelica keiskei, more widely known under the Japanese name of Ashitaba.
In folk medicine it is claimed to be diuretic, tonic, to improve digestion, and,
when applied topically, to speed wound healing and prevent infection. It also
improves the digestion system and strengthens the immune system with its
symbiotic micro organism content to strike a natural balance.
Anti-oxidant and wound healing
13
What is Bio-Tranquilizer?
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
Common name
INCI NAME
Scientific Name
Centella (Gotu Kola )
Centella asiatica leaf extract,
Centella asiatica
The plant is one of the best means of regenerative therapy and has for
centuries been used in the medicine of China and India. According to the
ancient records of oriental herbalists, it has been called one of the “miracle
elixirs of life”. It is used as a stimulating and toning remedy, to improve
metabolism and in the treatment of bronchitis, bronchial asthma and
tuberculosis
Wound healing, stimulate metabolism
14
What is Bio-Tranquilizer?
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
Common name
INCI NAME
Scientific Name
Magnolia
Magnolia liliflora flower extract
Magnolia liliflora
Magnolia is a large genus of about 210 flowering plant species in the
subfamily Magnolioideae of the family Magnoliaceae. It is only recently that
studies have highlighted the amazing health benefits of two of Magnolia's
constituents, honokiol and magnolol. Honokiol has an anti-anxiety effect
which is complemented by the anti-depressant effect of magnolol. Honokiol
and magnolol are both powerful antioxidants (in vitro, up to 1000 times
more potent than alpha tocopherol at inhibiting lipid peroxidation).
Anti-oxidant
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What Does Bio-Tranquilizer Do?
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
Anti- itching
Anti-allergy
Anti-irritation
Antiinflammation
Bio tranquilizer
1. Relives the inflammation (caused by irritation) by inhibiting immune response.
2. Suppresses the cytokines caused by inflammation.
3. Cures atopic dermatitis by controlling the immune system skin.
16
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
How well does BioTranquilizer perform?
Test Data
Lab Data
17
Human Patch Test
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
• Test Method(Human Patch Test)
Test material
Bio Tranquilizer
1. Volunteers
10 volunteers
aged 25~55
2. Applied Quantity
0.01ml
3. Method
• The finn-chamber containing 0.1% of SLS + 0.3%
DW and 0.1% of SLS + 0.3% of Bio Tranquilizer
were applied to a upper arm of volunteers for 24
hours and then was removed the Bio tranquilizer.
• After 24 hours from removal of the finn-chamber ,
skin condition was evaluated after 24hours of
removal of the Bio tranquilizer.
Male
7
Female
3
Human Patch Test
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
After application
Material
Control
(Distilled water)
Score
Test 1
(SLS 1% + Distilled water 3%)
Test 2
(SLS1% + Bio Tranquilizer 3%)
++
+
±
-
++
+
±
-
++
+
±
-
1
-
-
-
-
O
-
-
-
-
-
O
-
2
-
-
-
-
O
-
-
-
-
-
-
O
3
-
-
-
-
O
-
-
-
-
-
O
-
4
-
-
-
-
O
-
-
-
-
-
O
-
5
-
-
-
-
O
-
-
-
-
O
-
-
6
-
-
-
-
O
-
-
-
-
-
-
O
7
-
-
-
-
O
-
-
-
-
-
O
-
8
-
-
-
-
O
-
-
-
-
O
-
-
9
-
-
-
-
O
-
-
-
-
-
O
-
10
-
-
-
-
O
-
-
-
-
-
O
-
Test
number
A. Sample
a. Control : Distilled water
b. Test 1 : SLS 1% + Distilled water 3%
c. Test 2 : SLS1% + Bio Tranquilizer 3%
B. Visual grade
a. “-” : Negative reaction
b. “ ±” : Doubtful reaction
c. “+” : Weak positive reaction
d. “++” : Strong positive reaction
19
Positive Patch Test
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
• Sodium Lauryl Sulfate(SLS) is anionic surfactant/skin irritant
and inflammatory material when contacted with skin.
• Confirm the efficacy of BT-W
1. Distilled water
2. SLS 1% + Distilled water 3%
3. SLS 1% + BT-W 3%
• BT-W decreased irritation caused by SLS.
20
Positive Patch Test 2
•
Mehtod
–
–
–
Irritation was induced by attaching 0.5% SLS using finn-chamber for 24 hours.
0.5% SLS and finn-chamber were removed after 24 hours, and the product with natural anti-irritant
was applied twice a day(morning and night).
skin reaction was relieved after 7 days
7일 차
Control
1. 0.5% SLS
1.
2.
3.
4.
Control : Basic Lotion
BT-W 2%
BT-O 2%
BT-W + BT-O 2%
21
Human Patch Test
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
After application
Materi
al
Test 1
(Control : Basic Lotion)
Test 2
(BT-W 2%)
Test 3
(BT-O 2%)
Test 4
(BT-W 2% + BT-O 2%)
Score
Test
number
+++
++
+
±
-
+++
++
+
±
-
+++
++
+
±
-
+++
++
+
±
-
1
O
-
-
-
-
-
-
-
O
-
-
-
-
O
-
-
-
-
-
O
2
O
-
-
-
-
-
-
-
O
-
-
-
-
-
O
-
-
-
O
-
3
O
-
-
-
-
-
-
-
O
-
-
-
-
O
-
-
-
-
-
O
4
O
-
-
-
-
-
-
O
-
-
-
-
-
O
-
-
-
-
-
O
5
O
-
-
-
-
-
-
-
O
-
-
-
-
O
-
-
-
-
-
O
6
O
-
-
-
-
-
-
-
O
-
-
-
-
-
O
-
-
-
O
-
7
O
-
-
-
-
-
-
O
-
-
-
-
-
O
-
-
-
-
-
O
8
O
-
-
-
-
-
-
-
-
O
-
-
O
-
-
-
-
-
-
O
9
O
-
-
-
-
-
-
-
O
-
-
-
-
O
-
-
-
-
O
-
10
O
-
-
-
-
-
-
-
O
-
-
-
-
O
-
-
-
-
-
O
A. Sample
a. Test 1 :
b. Test 2 :
c. Test 3 :
d. Test 4 :
Basic Lotion
Basic Lotion + BT-W 2%
Basic Lotion + BT-O 2%
Basic Lotion + BT-W 2% + BT-O 2%
B. Visual grade
a. “-” : Negative reaction
b. “ ±” : Doubtful or slight reaction and erythema
c. “+” : Erythema + Induration
d. “++” : Erythema + Induration + Vesicle
e. “+++” : Erythema + Induration + Bullae
22
Sensory Skin Irritation Test Evaluation
Evaluation method of International Contact Dermatitis Research Group (ICDRG)
Score
±
+
++
+++
0
0.5
1
2
3
After application
Materi
al
Dermore
action
-
Test 1
(Control : Basic Lotion)
Test 2
(BT-W 2%)
Test 3
(BT-O 2%)
Test 4
(BT-W 2% + BT-O 2%)
+++
++
+
±
-
+++
++
+
±
-
+++
++
+
±
-
+++
++
+
±
-
10
-
-
-
-
-
-
2
7
1
-
-
1
7
2
*
-
-
3
-
Mean
Score
10
0.55
0.45
0.15
Grade
Strong irritation
No irritation
No irritation
No irritation
23
Anti-Inflammation
IL-6
Natural Anti-Irritant
Product Name:
LPS (Lipopolysaccharide) in HMC-1(Human mast cell line) .
12
IL-6 production (ng/ml)
•
Bio-Tranquilizer
10
8
6
4
2
0
LPS
LPS+BT-W 0.25%
LPS+BT-W 0.5%
Sample
LPS+BT-W 1%
LPS+BT-W 1.5%
IL-6 production started to decrease dramatically from 1% of BT-W.
24
NO Assay
Natural Anti-Irritant
Product Name:
Griess assay method
Using RAW 264.7 cell by LPS(Lipopolysaccharide) was used to stimulate RAW
264.7 cell.
100
90
80
Nitric Oxide(µ M)
•
•
Bio-Tranquilizer
70
60
50
40
30
20
10
0
LPS
LPS+BT-W 0.25%
LPS+BT-W 0.5%
LPS+BT-W 1%
LPS+BT-W 1.5%
Sample
NO expression stimulated by LPS decreased remarkably
from 1~1.5% of BT-W concentration in RAW 264.7 cell.
25
Beta-Hexosaminidase Assay
Natural Anti-Irritant
Product Name:
•
•
Bio-Tranquilizer
(PPDA was used to as an allergen in RAW264.7)
Inhibition of β-Hexosaminidase release was respectively as 100, 84.5, 57.0, 43.6,
32.1% in 0, 0.5, 1, 1.5, 2% of Bio Tranquilizer and it was decreased in a
concentration-dependent manner.
β-Hexosaminidase release(%)
100
80
60
40
20
0
0
0.5
1
BT-W Concentration(%)
BTW can be used as natural anti-histamine.
1.5
2
26
Antioxidative Effect
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
• DPPH Assay of Bio Tranquilizer
100
90
DPPH inhibition(%)
80
70
60
50
40
30
20
10
0
0
0.1
0.5
1
2
BT-W concentration (%)
• Bio tranquilizer showed anti-oxidant activity by concentration
dependent manner.
27
Safety-MTT Assay
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
• Cell viability
120
Cell viability(%)
100
80
60
40
20
0
0
0.5
1
2.5
5
BT-W Concentration(%)
7.5
10
Raw264.7 cell survival rate was not rarely changed even 10% of BT-W was
added in the rwa264.7 cell.
28
Physical Stability
Natural Anti-Irritant
Product Name:
Bio-Tranquilizer
•
Physical stability of Bio-Tranquilizer was evaluated
for 6 weeks. (4°C, room temperature, 45°C)
Result
Time(weeks)
Appearance
color
pH
1
Liquid
Light Brown
4.5
2
Liquid
Light Brown
4.5
4
Liquid
Light Brown
4.5
6
Liquid
Light Brown
4.5
BT-W
Before
4°C
Room
Temperature
BT-O
45°C
Before
4°C
Room
Temperature
No effects in
1. Quality
2. Color
3. pH
45°C
29
Skin Irritation
Natural Anti-Irritant
Product Name:
Bio-relaxer
Bio-tranquilizer
30
Market Size
Natural Anti-Irritant
Product Name:
Bio-relaxer
Bio-tranquilizer
Market size of Hypoallergenic CM(cosmetics market) and its forecast.
Atopic Market Forecast
y = 0.2832x + 7.7253
R² = 0.9998
Amount ( Billion in USD)
12
10
8
6
4
2
0
2012
2013
2014
2015
2016
Year
2017
2018
2019
2020
Source : Korea Institute of Oriental Medicine
Currently it is forecasted that the size of Atopic market is increasing at the rate of
approximately USD 0.28 billion/year from 2012 to 2020.
31
Improved
BT-W
( Lot15E01)
Improved BT-W
(Lot15E01)
 Refinement process improvement
 Same components with the existing BT-W
 Improved color
 Improvement of the smell of raw material (natural
substance)
 Improved abirritation effect
Experimental methods
 Cause irritation: Attach nonwoven fabric (2cmx2cm)
where 0.5g of 20% Lactic acid was dropped to Y zone,
and remove it after 2 minutes in order to cause irritation.
 Abirritation evaluation: Drop BT-W (Lot15E01) and
the product of B company respectively, by 0.5g onto the
same non-woven fabric (2cmx2cm), and evaluate
abirritation by attaching it to the left and right side of
the Y zone irritated by lactic acid.
Experimental results (Irritation evaluation)
Comparison
Irritation rating
-
±
BT-W
8
2
Product of
B company
7
1
(Lot15E01)
Control
group
+
++
1
1
6
+++
4