Typhidot Brochure - Reszon Diagnostics International
Transcription
Typhidot Brochure - Reszon Diagnostics International
TYPHOID FEVER DIAGNOSTIC Assay Procedure Typhoid fever is an infectious disease caused by bacterium, Salmonella typhi. It continues to be a major health problem especially in the Asia Pacific region, the Indian subcontinent, Central Asia, Africa and South America. Definitive clinical diagnosis of typhoid is unreliable because typhoid fever symptoms mimic other diseases with fever that are common in this part of the world. Clinical presentation very tremendously among patients and cover a wide spectrume, hence the need for a good laboratory test. Step 1 Add patient serum sample to pre-dotted antigen strip. Wash away unbound patient antibody. Step 2 Add HRP conjugated secondry antibody. Wash away unbond secondry antibody. Current methods to diagnose typhoid are by isolation of S. typhi from body fluids and by detection of antibody using the Widal test. Both methods are time-consuming with variable sensitivity. The culture method has a sensitivity of 48-78% but among the pediatric age group the sensitivity could be lower. Although the Widal test is widely used, its many limitations lead to difficulties in its interpretation especially in areas of high endemicity. Step 3 Add substatre and read results. We produce two types of diagnostics test kits for typhoid fever - Typhidot and Typhidot Rapid test kits. Typhidot is using a more sensitive Enzyme Immunoblot Assay; while Typhidot Rapid test kits are using a faster Immunochromatography Assay. Interpretation Of Result : Typhidot Typhidot is a revolutionary methos of diagnosis. Invented in Malaysia, it is the first known qualitative antibody detection test design for rapid diagnosis of typhoid fever. Using the Dot EIA technique, presence of IgM & IgG antibodies against a specific antigen on the outer membrane of S. typhi are detected. Typhidot has been evaluated by many endemic areas including Malaysia, India, Indonesia, Pakistan and Philippines. A control clinical field trial among febrile children admitted to a hospital in an endemic area showed that Typhidot when compared to culture is sensitive (95%) and did not show any cross-reactions. RAPID, SENSITIVE AND SPECIFIC!! Detects BOTH IgG and IgM - stages of infection can be determined simultaneously!! Please refer to product insert for detailed instruction. Features : Platform Format Detection : Enzyme Immunoassay : Dot Blot Strip : Human IgG and IgM against specific S. typhi 50kD OMP antigen Specimen : Serum Specimen Volume : 5 µl (1 hour method) 20 µl (3 hours method) Shelf Life : 12 months Assay Time : 1 or 3 Hours NO aaditional sample preparation required!! 95% sensitivity and DID NOT SHOW CROSS DETECTION RTF-ED0101 Typhidot 1 hour Salmonella typhi 28 T/ Kit RTF-ED0102 Typhidot 3 hours Salmonella typhi 28 T/ Kit Typhidot Rapid Test Kits Typhidot Rapid IgM Assay Procedure Step 1 Add serum/ plasma/ whole blood to square well. For whole blood this is followed by addition of one drop buffer Step 2 Up to area A, add 3 drops buffer to oval well. The test kits are designed for the qualitative detection and differentiation of specific IgM or IgG/ IgM antibodies against specific S. typhi antigen. Step 3 Pull clear plastic tab and add one drop buffer to square well FAST!! Results within 15 minutes Interpretation Of Result : Results are EASY to INTERPRET - test line appears means POSITIVE RESULTS MINIMAL sample volume is required Features : Platform Format Detection Specimen Specimen Volume Shelf Life Assay Time Typhidot Rapid IgG & IgM (combo) Assay Procedure : Immunochromatography assay : Cassette : Human IgM or IgG/IgM against S.typhi OMP antigen : Serum/ Plasma or Whole blood : 30-35 µl : 18 months : 15 minutes TYPHIDOT RAPID IgM IgG T Step 1 Add serum/ plasma to each sample well. T Step 2 Up to area C, add one drop buffer to each well. Read result within 15-20 minutes. TYPHIDOT RAPID IgM IgG T T Interpretation Of Result : Positive for IgG & IgM Positive for IgG IgM IgG C T C T C T IgM IgG C T C T Negative C T Invalid IgM IgG IgM IgG IgM IgG C T Positive for IgM IgM IgG C T C T C T C T C T Please refer to product insert for detailed instruction. Ordering Information : RTF-RD0101 Typhidot Rapid IgM Salmonella typhi 25 T/ Kit RTF-RD0202 Typhidot Rapid IgG IgM (combo) Salmonella typhi 25 T/ Kit Reszon Diagnostics International Sdn. Bhd. 917901-W Revongen Corporation Center, No.12A, Jalan TP5, Taman Perindustrian UEP, 47600 Subang Jaya, Selangor Darul Ehsan, Malaysia. T : +6 03 8025 1603 F : +6 03 8025 1637 E : info@reszonics.com W: www.reszonics.com