poster sessions | sunday | august 2

Transcription

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POSTER SESSIONS | SUNDAY | AUGUST 2
Theme 01 – Exercise Physiology...............................................................01.001 – 01.019
Theme 02 – Renal Physiology..................................................................02.001 – 02.008
Theme 03 – Neurophysiology..................................................................03.001 – 03.020
Theme 04 – Cardiovascular Physiology.......................................................04.001 – 04.036
Theme 05 – Gastrointestinal Physiology.....................................................05.001 – 05.004
Theme 06 – Celular Physiology................................................................06.001 – 06.003
Theme 07 – Comparative Physiology.........................................................07.001 – 07.005
Theme 08 – Respiratory Physiology...........................................................08.001 – 08.015
Theme 09 – Endocrine & Metabolism.........................................................09.001 – 09.020
Theme 10 – Physiology Education.............................................................10.001 – 10.007
THEME 01 - EXERCISE PHYSIOLOGY
01.001 - EFEITOS DO EXERCÍCIO FÍSICO E DO LPS NO REPARO ÓSSEO
JONATAS EVANDRO NOGUEIRA1; LUIZ GUILHERME DE SIQUEIRA BRANCO2; JOÃO PAULO MARDEGAN
ISSA2
1.ESCOLA DE EDUCAÇÃO FÍSICA E ESPORTES DE RIBEIRÃO PRETO - USP, RIBEIRAO PRETO, SP,
BRASIL; 2.FACULDADE DE ODONTOLOGIA - USP, RIBEIRAO PRETO, SP, BRASIL.
O reparo ósseo é um processo que consiste na restauração dos tecidos lesados, determinado por
vários eventos biológicos para se obter a cura. Pode ser facilitado por meio de enxertos,
estimulação bioquímica e estimulação física; e retardado por outros agentes como a endotoxina
(LPS). O exercício físico (EF) exerce efeito benéfico para o osso, porém não é conhecido seu efeito
sobre a reparação óssea. Investigamos o efeito do EF sobre a ação do LPS no reparo ósseo, por
meio da densitometria óssea, análise histológica quantitativa do tecido ósseo neoformado e
marcadores imuno-histoquímicos, em animais sedentários e treinados. O EF consistiu no
treinamento na esteira durante 4 semanas. Após o treinamento, os ratos foram submetidos à
cirurgia para realização do defeito ósseo na tíbia direita e 24 horas após a cirurgia, o LPS foi
administrado na dose de 100 µg/kg ou 1 ml/kg de salina, i.p. Após um período de 10 dias da
cirurgia e sem EF, as tíbias direitas foram obtidas para análises. Os ratos treinados tiveram menor
peso corporal do que os ratos sedentários (P<0,001). O EF exerceu efeito positivo na reparação
óssea, aumentando a densidade mineral óssea (P<0,005), o conteúdo mineral ósseo (P<0,005), a
neoformação óssea (P<0,005), o colágeno tipo I (P<0,05) e a expressão de osteocalcina (P<0,05).
Esses parâmetros não foram afetados pela administração sistêmica de LPS. Os dados indicam que
o EF exerce um importante efeito osteogênico, que é mantido durante a inflamação sistêmica
aguda induzida por uma única dose de LPS.
01.002 - RESISTANCE EXERCISE INCREASES MAXIMUM STRENGTH AND INSULIN SENSITIVITY AND
REDUCES BLOOD GLUCOSE IN PINEALECTOMIZED RATS
MARIANA LOPES BENITES; LEONARDO RANIEL FIGUEIREDO; THAÍS SAORI TSOSURA; NATALIA
FRANCISCO SCARAMELE; MURILO CARETA GUIMARÃES; RENATO FELIPE PEREIRA; FERNANDO
YAMAMOTO CHIBA; DORIS HISSAKO SUMIDA
UNESP, ARACATUBA, SP, BRASIL.
Introduction: Insulin helps in protein syntesis, repair of damaged fibers and growth of skeletal
muscles after physical exercise. Insulin action may be improved by melatonin (ME). Individuals
exposed to light in nighttime inhibit the synthesis and secretion of ME by pineal gland. Objective:
It was evaluated the effect of pinealectomy and resistance exercise (RE) on the maximal strenght,
blood glucose and insulin sensitivity in rats. Methods: The animals were divided into 4 groups:
sedentary; exercited (in ladder for 8 weeks); sedentary pinealectomized (pinealectomy performed
at 40 days of life); pinealectomized exercited. All the animals were subjected to the inicial and
final maximal strenght test. The exercited animals performed maximal strenght test every 15
days. The training consisted of 3 weekly sessions of 9 climbs ladder with 60% of the maximum load
and 120 seconds of interval. After 8 weeks, blood samples were collected from fasted animals to
perform the insulin tolerance test. Results: The pinealectomy increased blood glucose levels of
sedentary animals (p<0,05). RE decreased blood glucose levels of pinealectomised animals
(p<0,05) and increased the maximum strenght and sensitivity to insulin in pinealectomized and
non pinealectomized animals (p<0,05). Conclusion: It was observed that, resistance exercise
practice was able to increase muscle strenght and insulin sensitivity in rats, with or without pienal
gland, and prevent hyperglyemia observed in pinealectomized animals.
01.003 - RECOVERY OF FORCE PRODUCTION IS RELATED TO CD14+CD16- PHENOTYPE AND
ANTICIPATED P47PHOX EXPRESSION IN BLOOD MONOCYTES OF INDIVIDUALS SUBMITTED TO
ECCENTRIC EXERCISE INDUCED MUSCLE DAMAGE
HENVER SIMIONATO BRUNETTA; VILMA SIMÕES PANZA; CAROLINA DE QUADROS CAMARGO; BRUNO
MONTEIRO DE MOURA; RAPHAEL LUIZ SAKUGAWA; AMANDA MARREIRO BARBOSA; THAYZ
RODRIGUES CHAGAS; MARINA VIEIRA DE OLIVEIRA; FERNANDO DIEFENTHAELER; EDSON LUIZ DA
SILVA; EVERSON ARAÚJO NUNES
FEDERAL UNIVERSITY OF SANTA CATARINA, FLORIANOPOLIS, SC, BRASIL.
This study evaluated the phenotype and expression of the p47 phox protein (a NADPH oxidase
subunit) in blood monocytes and its relation to the force production capacity during the recovery
from skeletal muscle damage induced by eccentric exercise. Fourteen male, aged 24±3 years,
body mass 72.3±10.8kg and body fat of 15.3±4.5% participated in the study. The muscle damage
protocol consisted of three sets of 20 repetitions with intervals of 2 min using the elbow flexor,
eccentric phase only, at a isokinetic dynamometer at 45°•s-1. Blood and peak torque were
collected and assayed immediately before (baseline (B)), 24, 48 and 72h after the damage
protocol. Part of the individuals (n=7) recovered more than 80% (Rec>80%) of the force generation
capacity (N•m) in 72h after the damage (B=65.9±10.9 vs. 72h=58.9±8.4). Another group (Rec<80%)
(n=7), did not reach 80% recovery (B=72.5±8.4 vs. 72h=50.1±8.9) and presented lower baseline
percentage of CD14+CD16- monocytes than the Rec>80% group (90.2±5.4% vs. 95.9±1.6%, p<0.05).
There was an increase in the expression of p47phox in CD14+ cells in the Rec>80% 24h after damage
(p<0.05). In the Rec<80%, this increment occurred at 48h and 72h, but only compared to the 24h.
The increased prevalence of CD14 +CD16- cells and the anticipated increase in p47 phox expression
seem to contribute to the early recovery of force generation capacity after eccentric damage.
01.004 - AEROBIC TRAINING PREVENTS IMPAIRMENT IN VASCULAR REACTIVITY IN RATS UNDER
HIGH RISK OF METABOLIC SYNDROME DEVELOPMENT
TAMIRIS MIKALOSKI BUENO GOMES; RAQUEL KINDLOVITS; RENATA FRAUCHES MEDEIROS; THAIANE
GADIOLI GAIQUE; THAIS BENTO-BERNARDES; NADIA ALICE VIEIRA MOTTA; FERNANDA CARLA
FERREIRA BRITO; KAREN JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA NÓBREGA
UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRASIL.
It’s not totally elucidated the mechanism through which the aerobic training could prevent the
clinical disease appearance. So the purpose was to investigate the mechanism of aerobic training
on vascular reactivity of rats under high risk of metabolic syndrome development. Wistar rats,
males, adults, were divided in Control group (C; drinking water), Fructose group (F; water with
10% fructose), both for 10 weeks; Control Training group (CT) and Fructose Training group (FT),
adding aerobic training on a treadmill in the last 8 weeks. Triacylglycerol and malondialdehyde
was higher only in F group and was similar between FT, CT, and C groups. NO was higher in trained
groups when compared to sedentary groups. In addition, the F group exhibited increased
vasoconstrictory reactivity to phenylephrine when compared to C and FT. The F group also
presented an impaired endothelial vasodilator response to acetylcholine compared to C and FT
vasodilatory response was similar to C and CT groups. What concerns about eNOS quantification
it was observed that F group had lower expression when compared to C group and the groups that
trained had higher values when compared to their sedentary controls, there was no difference
between CT and FT groups. In relation to eNOSp it was observed that the trained groups (P=0.03)
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had higher values when compared to sedentary groups. So aerobic exercise prevents damage in
vascular reactivity, increases eNOS and eNOSp expression in this model.
01.005 - THE PHYSICAL EXERCISE-INDUCED CUTANEOUS HEAT LOSS DEPENDS ON THE
NONTHERMAL ACTIVATION OF SYSTEMIC TRVP1 CHANNELS
ANA CANCADO KUNSTETTER; WILLIAM COUTINHO DAMASCENO; DANUSA DIAS SOARES; NILO
RESENDE VIANA LIMA; SAMUEL PENNA WANNER
UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL.
OBJECTIVE: The transient receptor potential channel - vanilloid subtype 1 (TRPV1) is a polymodal
channel activated by noxious heat, capsaicin, and protons. The activation of systemic TRPV1
channels triggers cutaneous heat loss during exercise; however, it has not been determined which
mode of TRPV1 activation is involved in this thermoregulatory response and, therefore, this was
the objective of the present study.
METHODS: The study was approved by a local ethics committee (protocol 076/2011). Male adult
Wistar rats were injected with one of the following three TRPV1 antagonists: AMG517 (60 g/kg
iv) or ABT-102 (3 mg/kg iv), which block all modes of TRPV1 activation, or A1165442 (3 mg/kg iv),
which does not block the proton mode of TRPV1 activation. The rats were then subjected to an
incremental-speed exercise on a treadmill under temperate conditions (24°C) and their brain and
tail skin temperatures were measured while they were running.
RESULTS: Compared with the control experiments, the acute injection of AMG 517 or ABT-102
increased the cutaneous heat loss threshold by ~1C, exaggerating the brain hyperthermia induced
by exercise. However, the injection of A1165442 did not change either the cutaneous heat loss
threshold or the exercise-induced increase in brain temperature.
CONCLUSION: The activation of systemic TRPV1 channels by a nonthermal stimulus increases
cutaneous heat loss, thereby avoiding exaggerated brain hyperthermia during exercise.
01.006 - REMOTE ISCHEMIC PRECONDITIONING IMPROVES RAPID ONSET VASODILATION TO
SINGLE SKELETAL MUSCLE CONTRACTION IN HUMANS
DANIEL ELIAS DE JESUS MANSUR 1; THALES COELHO BARBOSA1; THIAGO RIBEIRO LOPES2; LAURO
CASQUEIRO VIANNA3; ANTONIO CLAUDIO LUCAS DA NÓBREGA1; BRUNO MOREIRA SILVA2
1.FLUMINENSE FEDERAL UNIVERSITY, NITEROI, RJ, BRASIL; 2.FEDERAL UNIVERSITY OF SÃO PAULO,
SÃO PAULO, SP, BRASIL; 3.UNIVERSITY OF BRASILIA, BRASILIA, DF, BRASIL.
Remote ischemic preconditioning (RIPC) affects vascular function at rest and flow-mediated
dilation. We investigated the effect of RIPC on the hyperemic response after single skeletal muscle
contraction. Twelve men (25±1 years) underwent RIPC (3 cycles of 5 min occlusion/ 5 min
reperfusion of the legs; thigh cuffs inflated to 200 mmHg) and a control procedure (CT; 10 mmHg)
in random order on two visits. After each procedure, they performed brief (>1 s) static handgrip
contractions, in supine position with 2-min interval. Beat-to-beat blood pressure (BP), forearm
blood flow (FBF) and vascular conductance (FVC; FBF/BP) before (BL) and for 15 cardiac cycles
after each contraction were averaged from 3 contractions. Muscle contractions increased FBF and
FVC within 3 to 4 cardiac cycles (62% and 60% change from BL, respectively FBF and FVC). Peak
increase in FBF and FVC was similar between RIPC and CT (P > 0.05), but the following decay was
attenuated by RIPC, such that FBF was ~12% higher than CT between the 9th-15th cardiac cycles
(P < 0.05 vs. CT) and FVC was ~15% higher than CT between the 8th-15th cardiac cycles (P < 0.05
vs. CT). Our findings demonstrate that RIPC prolongs the vasodilator effect of a single contraction.
01.007 - EFEITO AGUDO DA ESTIMULAÇÃO TRANSCRANIANA DE CORRENTE CONTÍNUA NO
NÚMERO TOTAL DE REPETIÇÔES E PERCEPÇÃO DE FADIGA MUSCULAR
RODRIGO CUNHA DE MELLO PEDREIRO1; EDUARDO LATTARI 2; MARIA LÚCIA ANDRADE1; ALBERTO
SOUZA DE SÁ FILHO2; ANTÔNIO MARCOS MOURA2; SAMARA SEZANA COSTA1; GERALDO
ALBUQUERQUE MARANHÃO NETO1; SÉRGIO MACHADO2
1.UNIVERSIDADE SALGADO DE OLIVEIRA, CORDEIRO, RJ, BRASIL; 2.UNIVERSIDADE FEDERAL DO RIO
DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL.
O objetivo do estudo foi avaliar o efeito agudo da estimulação transcraniana de corrente continua
anódica (ETCC-a) sobre o volume total de repetições e percepção subjetiva do esforço em
indivíduos praticantes de treinamento força (TF). A amostra foi de 10 sujeitos treinados. Os
mesmos foram submetidos a exercício de flexão de cotovelo na barra com carga específica de
10RM, imediatamente após, responderam a escala OMNI-RES e foram estimuladas durante 20
minutos com um protocolo ETCC, dependendo da randomização. Após a ETCC, novamente foram
submetidos a realizar o mesmo exercício e responder a escala OMNI-RES. Foram três condições
experimentais, a ETCC catódica (ETCC-c), ETCC-a e ETCC placebo (ETCC-p). Houve um intervalo
de 48 a 72 horas entre as visitas. Uma interação de condições e tempo (F = 52,395; p≤0,001)
mostrou que repetições completadas após ETCC-a foram maiores em comparação com as outras
condições realizadas também após. Em relação à percepção de esforço, a ANOVA de duas entradas
para medidas repetidas mostrou uma interação entre condição e tempo (F = 28,445; p≤0,001),
onde o esforço percebido foi diminuído após a condição ETCC-a e aumentada após a condição
ETCC-c. Em suma, parece ser benéfico realizar uma sessão de 20 minutos ETCC-a para
potencializar o rendimento de indivíduos praticantes de TF, assim como reduzir a percepção de
fadiga.
01.008 - EFFECT OF ISCHEMIC PRECONDITIONING ON AEROBIC PERFORMANCE IN HIGH-LEVEL
RUNNERS: A RANDOMIZED AND PLACEBO/NOCEBO CONTROLLED STUDY
JEANN LUCCAS DE CASTRO SABINO-CARVALHO1; THIAGO RIBEIRO LOPES2; THIAGO HENRIQUE NUNES
FERREIRA1; TIAGO OBEID-FREITAS1; JOSE ERNESTO SUCCI1; ANTONIO CARLOS SILVA1; BRUNO
MOREIRA SILVA1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL; 2.CENTRO OLÍMPICO DE
TREINAMENTO E PESQUISA, SÃO PAULO, SP, BRASIL.
It has been reported that ischemic preconditioning (IPC; brief cycles of ischemia) enhances aerobic
performance. But, placebo/nocebo effects have not been adequately controlled. Moreover, no
study assessed high-level runners or used strict criteria to confirm the maximal oxygen uptake
(VO2max). Thus, we submitted 11 high-level runners to three interventions, in random order (IPC;
SHAM, fake ultrasound session; CT, resting control). Subjects were told that both IPC and SHAM
would improve performance compared to CT (i.e., we sought to induce a similar placebo, while
avoiding a nocebo effect). After each intervention, gas exchange, blood lactate [La] and rating of
perceived exertion (RPE) were measured during a maximal incremental test. Ten min later, a
supramaximal constant test was used to verify the VO2max. Most subjects believed at pre-test
that IPC and SHAM would improve performance (73 and 82%, respectively). Running oxygen cost
(P = 0.80), VO2max (P = 0.44), RPE (P = 0.42) and lactate threshold (P = 0.57) were similar among
interventions. Peak velocity in the maximal test (P = 0.28) and time to exhaustion in the
supramaximal (P = 0.34) were not altered. In summary, these preliminary results indicate that IPC
did not improve aerobic performance in high-level runners. Thus, the ergogenic effect reported
by previous studies in intermediate-level runners suggests that aerobic fitness may modulate the
IPC effect on aerobic performance.
01.009 - THE INCREASE IN GUT PERMEABILITY INDUCED BY EXERCISE DEPENDS ON THE
MAGNITUDE OF HYPERTHERMIA
ALEXANDRE SERVULO RIBEIRO HUDSON; ANNE DANIELI NASCIMENTO SOARES; CRISTIANO ANTÔNIO
MACHADO-MOREIRA; DANUSA DIAS SOARES; VALBERT NASCIMENTO CARDOSO; CÂNDIDO CELSO
COIMBRA; SAMUEL PENNA WANNER
Gut permeability increases as a consequence of exercise-induced hyperthermia, leading to
endotoxin leakage and an inflammatory response. In more severe cases, endotoxemia can cause
multiple-organ failure and death. Thus, we investigated whether the magnitude of hyperthermia
induced by prolonged exercise affects intestinal permeability. Twenty-nine male Wistar rats were
divided in four groups: rest at 24°C or constant-speed treadmill running (90 min, 15 m/min) at
13ºC (cold), 24°C (temperate) and or 31°C (warm). Thirty min prior to the experiments, the rats
received 0.1 ml of diethylenetriaminepentaacetic acid solution labeled with 18.5 MBq of 99mTc
by gavage. After the experiments, radioactivity was counted in blood samples and used as an
index of intestinal permeability. The abdominal temperature of the rats was not changed by
constant exercise at 13°C, but attained average values of 39.07 ± 0.35°C at the end of exercise
at 24°C. No changes in the intestinal permeability were observed between the exercise groups at
13°C and 24°C and the rest group at 24°C. The rats subjected to exercise at 31°C showed
abdominal temperature values that exceeded 40°C and remained high for a prolonged period. In
this group, intestinal permeability was higher than in the other three groups. Therefore, the
magnitude of hyperthermia affects exercise-induced intestinal permeability, which was only
observed when rats showed abdominal temperatures exceeding 40ºC.
01.010 - EFFECT OF DIFFERENT TYPES OF AEROBIC TRAINING ON PHYSICAL PERFORMANCE AND
THERMOREGULATORY RESPONSES IN RATS SUBJECTED TO AN INCREMENTAL EXERCISE IN THE
HEAT
FRANCISCO TEIXEIRA COELHO; FILIPE FERREIRA VAZ; CLETIANA GONÇALVES DA FONSECA;
WASHINGTON PIRES; DANUSA DIAS SOARES; SAMUEL PENNA WANNER
Aerobic training induces several adaptations in the thermoregulatory system, including a decrease
in the core body temperature threshold for cutaneous vasodilation during exercise, which may
improve human performance in a hot environment. This study investigated the effect of different
types of aerobic training conducted in a temperate environment on the performance and
thermoregulatory responses of rats during an exercise in the heat. Adult male Wistar rats (n = 32)
were divided into four groups: a control group (C) and three groups that were subjected to a 8week aerobic training, during which the physical overload was achieved by predominantly
increasing the exercise intensity (I), duration (D) or by increasing both (ID). After the training
protocol, the core and tail skin temperatures of the rats were measured during an incremental
treadmill running (initial speed of 10 m/min, which was increased by 1 m/min every 3 min until
volitional fatigue) in a hot environment (32°C). All groups subjected to the aerobic training
showed improved performance (I: 48±3 min; D: 47±4 min; ID: 46±2 min; p < 0.05) compared with
the C group (34±2 min). There were no differences in the core and tail temperatures among the
groups studied. These findings suggest that the three types of training increased aerobic
performance in the heat by mechanisms that were independent of thermoregulatory adaptations.
01.011 - CHRONIC AEROBIC EXERCISE RESTORE MICROVASCULAR FUNCTION IN RATS WITH
METABOLIC SYNDROME
ROBERTA FRANCO BARBOSA PEÇANHA; FLAVIA GARCIA DE OLIVEIRA; MARCUS VINICIUS MACHADO;
ELIETE DALLA CORTE FRANTZ; ALESSANDRA CHOQUETA DE TOLEDO ARRUDA; ANTONIO CLAUDIO
LUCAS DA NÓBREGA
UFF, NITEROI, RJ, BRASIL.
Introduction: Metabolic syndrome (MS) is related to systemic functional microvascular alterations
including a significant reduction in microvessel density.
Aim: The aim of the study was to investigate the effects of exercise training on functional capillary
density in the skeletal muscle and skin of rats with MS.
Methods: Male Wistar-Kyoto rats were fed a standard commercial diet (CON) or high-fat diet (HFD)
for 32 weeks. Animals receiving the HFD were randomly divided into sedentary (HFD+SED) and
training groups (HFD+TR) at the 20th week. After 12 weeks of aerobic treadmill training, the
maximal oxygen uptake (VO2max); hemodynamic, biochemical and anthropometric parameters;
and functional capillary density were assessed.
Results: Exercise training increased the VO2max (p<0.05) and exercise tolerance (p<0.05)
compared with the HFD+SED and CON. The HFD+TR also reduced visceral fat (p<0.001) and systolic
blood pressure (127±2 vs. 150±2 mmHg, p<0.001). Moreover, exercise improved glucose tolerance,
as evaluated by an intraperitoneal glucose tolerance test (p<0.05), fasting plasma glucose
(p<0.001) and fasting plasma insulin levels (p<0.05) compared with HFD+SED. Exercise training
also increased the number of spontaneously perfused capillaries in the skeletal muscle (252±9 vs.
207±9 capillaries/mm2) compared with HFD+SED.
Conclusion: Exercise training reversed skeletal muscle and skin capillary rarefaction in our
experimental model of MS and obesity.
Key words: Metabolic syndrome; Exercise; Microvascular density
01.012 - AEROBIC EXERCISE MODULATION OF MENTAL STRESS-INDUCED RESPONSES IN
CULTURED ENDOTHELIAL PROGENITOR CELLS FROM HEALTHY AND METABOLIC SYNDROME
SUBJECTS
HELENA NALY MIGUENS ROCHA1; NATÁLIA GALITO ROCHA1; ALLAN ROBSON KLUSER SALES1; BRUNO
MOREIRA SILVA2; ALINE ARAÚJO RABELO1; ANTONIO CLAUDIO LUCAS DA NÓBREGA1
1.UNIVERSIDADE FEDERAL FLUMINENSE, NITEROI, RJ, BRASIL; 2.UNIVERSIDADE FEDERAL DE SÃO
PAULO, SÃO PAULO, SP, BRASIL.
Studies have shown that exercise acutely prevents the reduction in flow-mediated dilation induced
by mental stress in metabolic syndrome (MetS). We investigated whether exercise protects from
the deleterious effect of mental stress on cultured endothelial progenitor cells (EPC) in healthy
subjects and those with MetS. Ten healthy subjects (aged 31±2) and ten subjects with MetS (aged
36±2) underwent a mental stress test, followed by either 40 min of leg cycling or rest across two
randomized sessions: mental stress+non-exercise control (MS) and mental stress+exercise
(MS+EXE). Stroop Color-Word test was used to elicit mental stress. Blood samples were drawn at
baseline and following sessions to isolate mononuclear cells. They were cultured in fibronectincoated plates for seven days, and EPC were identified by immunofluorescence (acLDL+/UEA-1
Lectin+). All subjects presented similar increases in mean blood pressure and heart rate during
mental stress test (P<0.01) in both the MS and MS+EXE. EPC number was not different between
groups at baseline in both sessions (P>0.05). EPC response to MS and MS+EXE was increased in
healthy subjects, whereas it was decreased in subjects with MetS (P<0.04). In healthy subjects,
the EPC response to MS+EXE was greater than the response to MS alone (P=0.03). An exercise
session increased EPC in healthy subjects but did not prevent the EPC reduction induced by mental
stress among subjects with MetS.
01.013 - HEPATIC RENIN–ANGIOTENSIN SYSTEM IS MODULATED BY EXERCISE TRAINING IN
FRUCTOSE-FED RATS
JULIANA BITTENCOURT SILVEIRA DE LIMA; ISABELE GOMES GIORE; ELIETE DALLA CORTE FRANTZ;
RENATA FRAUCHES MEDEIROS; THAIS BENTO-BERNARDES; THAIANE GADIOLI GAIQUE; CAROLINE
FERNANDES SANTOS; KAREN JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA NÓBREGA
Introduction: The renin-angiotensin system (RAS) has been implicated in the development of
nonalcoholic fatty liver disease (NAFLD). We investigated whether the hepatic RAS is modulated
by exercise training and if this modulation improves NAFLD.
Methods: Male Wistar rats were divided into (n = 8 each) control (CT), exercise control (CT-Ex),
high fructose (HFr) and exercise high fructose (HFr-Ex; fructose in drinking water) groups. After
two weeks, CT-Ex and HFr-Ex rats were assigned to a treadmill training protocol at moderateintensity for 8 weeks (60 min/day, 4 days/week). We assessed body mass, lipid metabolism,
hepatic histopathology and the expression profile of components of the RAS. The differences
between the groups were tested by ANOVA and the post hoc Holm–Sidak test.
Results: Neither fructose overload nor exercise training influenced body mass gain. Exercise
training was effective in preventing hepatic steatosis as well as local and systemic triglyceride
accumulation. The HFr rats displayed increased hepatic ACE protein expression which was
attenuated by exercise training. Exercise training restored the ACE2/Mas receptor axis and
decreased the ACE/ACE2 ratio and the AT1R/Mas receptor ratio towards normal levels in liver.
Conclusion: Exercise training may favor the counter-regulatory ACE2/Mas receptor axis over the
classical RAS (ACE/AT1R axis), which could be responsible for the prevention of NAFLD.
Key words: Renin-angiotensin system (RAS); Exercise training; Fructose; Non-alcoholic fatty liver
disease (NAFLD).
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01.014 - ROLE OF AEROBIC TRAINING ON LEFT VENTRICULAR HYPERTROPHY IN A HIGH
FRUCTOSE EXPERIMENTAL MODEL
RAQUEL KINDLOVITS; TAMIRIS MIKALOSKI BUENO GOMES; RENATA FRAUCHES MEDEIROS; KAREN
JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA NÓBREGA
The high fructose intake is associated with ventricular hypertrophy, while training is responsible
for physiological hypertrophy. However, it is not known if training can prevent this alteration
caused by high fructose diet. Thus, the purpose of this study was to investigate the effect of
aerobic training on ventricular hypertrophy alterations caused by a high fructose intake. Wistar
rats, males, adults, were divided in Control group (C; drinking water), Fructose group (F; water
with 10% fructose), both for 10 weeks; Control Training group (CT) and Fructose Training group
(FT), adding aerobic training, in the last 8 weeks. At the end of treatment metabolic variables,
body lipid, left ventricle (LV) weigh and PTP1B protein expression in LV were analyzed.
Triglycerides (TG), isoprostane and insulin were higher only in F group and were similar between
others groups. This same pattern was observed in relation to lipid body content. In addition, CT,
F and FT groups presented higher LV weight when compared to C group. The maximal aerobic
capacity was lower in F group compared to others. The PTP1B protein expression, responsible for
inhibit insulin signaling pathway, had tendency to be higher (47%) on F group, when compared to
C group, however C and FT groups demonstrated similar values. In conclusion, though aerobic
training increased LV weight, it was able to prevent alterations in body lipid content, maximal
aerobic capacity, insulin, TG, oxidative stress and in PTP1B expression.
01.015 - EFFECTS OF ACUTE SUPPLEMENTATION WITH GLUTAMINE ON PHYSICAL PERFORMANCE
IN A PROLONGED EXERCISE IN THE HEAT
DIOGO ANTONIO SOARES PACHECO1; DANUSA DIAS SOARES2; IULA LAMOUNIER LUCCA1; MARCELO
TEIXEIRA DE ANDRADE1; ALEXANDRE SERVULO RIBEIRO HUDSON1; YGOR ANTONIO TINOCO
MARTINS1; WESLLEY MOURA SANTOS1; NICOLAS HENRIQUE SANTOS BARBOSA1; CRISTIANO ANTÔNIO
MACHADO-MOREIRA3; CÂNDIDO CELSO COIMBRA1; SAMUEL PENNA WANNER1
1.FEDERAL UNIVERSITY OF MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE
FEDERAL DE MINAS GERAIS, MINAS GERAIS, MG, BRASIL; 3.FEDERAL UNIVERSITY OF JUIZ DE FORA,
GOVERNADOR VALADARES, MG, BRASIL.
This study evaluated the effects of acute supplementation with glutamine (GLN) on physical
performance in a prolonged exercise in the heat. Nine healthy subjects participated in the study.
On the first visit to the laboratory, a maximum progressive test was conducted to measure the
maximum power output (PMAX; 210 ± 30 W) attained by the volunteers, followed by a
familiarization exercise trial in the heat. In the experimental trials, volunteers were
supplemented with GLN, glycine (GLY) or a placebo drink (PLA) and then exercised until volitional
fatigue (started cycling at 50% of PMAX, which was increased by 1% every 8 min) in a hot
environment (34 C; 63% RH). The supplementation with GLN induced no ergogenic effect on
prolonged exercise in the heat (PLA: 68.4 ± 6.7 min vs. GLY: 68.4 ± 5.6 min vs. GLN: 69.6 ± 5.0
min; p = 0.916), although some volunteers displayed marked improvements in performance
(maximum increase of 30%) and others displayed marked impairments (maximum reduction of
19%). In addition, the change in performance caused by GLN showed a negative correlation with
performance in the PLA trial (r = -0.79; p < 0.01). Our data indicate that acute supplementation
with GLN did not influence physical performance during cycling in the heat; although a great
individual variability was observed. Moreover, we provide preliminary evidence that
supplementation with GLN may induce ergogenic effects in individuals who have low performance
in the heat.
01.016 - CARDIOPULMONARY AFFERENTS MODULATE HEART RATE RESPONSIVENESS TO MUSCLE
METABOREFLEX ACTIVATION IN HUMANS
MAYARA C SOUZA1; JAMES P FISHER2; LAURO CASQUEIRO VIANNA1
1.UNIVERSITY OF BRASÍLIA, BRASILIA, DF, BRASIL; 2.UNIVERSITY OF BIRMINGHAM, BIRMINGHAM,
REINO UNIDO.
Human studies have shown that muscle metaboreflex activation-mediated increases in heart rate
are principally attributable to increased cardiac sympathetic activity. The loading of
cardiopulmonary baroreceptors, associated with the supine posture, also provides
neuromodulatory signals to the brainstem and elicits cardiac sympathoinhibition. We sought to
determine whether cardiopulmonary loading modulates the HR response to muscle metaboreflex
activation in humans. In 14 healthy men (20±1 yrs) oscillometric blood pressure (Microlife 3BTOA) and heart rate (HR; Polar RS800) were measured during post-exercise ischemia (PEI; muscle
metaboreflex activation) with concurrent cardiopulmonary loading (supine posture) or unloading
(seated posture). At rest, the shift from supine to seated posture increased HR from 64±2 to 69±2
beats.min-1 (P<0.05), whereas blood pressure decreased from 90±2 to 84±2 mmHg (P<0.05). PEI
provoked a robust increase in blood pressure from rest (∆36±3 mmHg, P<0.05), which was not
affected by body posture. During PEI with cardiopulmonary unloading, HR was elevated from rest
(∆9±3 beats.min-1, P<0.05), but blunted with cardiopulmonary loading (∆0.9±2 beats.min-1,
P>0.05 vs rest). Thus HR responsiveness to muscle metaboreflex activation is importantly
modulated by afferent input from cardiopulmonary baroreceptors, suggesting that the
cardiopulmonary baroreflex and muscle metaboreflex interact to control cardiac autonomic
activity.
01.017 - EFFECTS OF ACUTE SUPPLEMENTATION WITH GLUTAMINE ON A VIGILANCE TASK
PERFORMANCE AFTER A PROLONGED EXERCISE IN THE HEAT
DIOGO ANTONIO SOARES PACHECO1; DANUSA DIAS SOARES2; RÚBIO SABINO BRUZZI1; IULA
LAMOUNIER LUCCA1; MARCELO TEIXEIRA DE ANDRADE1; DOUGLAS SILVA BERNARDES1; CHRISTIANO
EDUARDO VENEROSO3; CRISTIANO ANTÔNIO MACHADO-MOREIRA4; CÂNDIDO CELSO COIMBRA1;
SAMUEL PENNA WANNER1
1.UNIVERSIDA FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE
FEDERAL DE MINAS GERAIS, MINAS GERAIS, MG, BRASIL; 3.UNIVERSIDADE FEDERAL DO MARANHÃO,
SÃO LUÍS, MG, BRASIL; 4.UNIVERSIDADE FEDERAL DE JUIZ DE FORA, JUIZ DE FORA, MG, BRASIL.
This study evaluated the effects of acute supplementation with glutamine (GLN) on a vigilance
task performance after a prolonged exercise in the heat. Nine healthy subjects participated in
the study. On the first visit to the laboratory, they performed a maximal progressive exercise test
to measure the maximum power output (PMAX; 210 ± 30 W), followed by a familiarization exercise
trial and a psychomotor vigilance test (PVT). In the experimental trials, the volunteers initially
performed the PVT and then ingested a drink solution containing GLN or glycine (GLY) or placebo
drink. One hour later, the volunteers exercised until volitional fatigue (started cycling at 50% of
PMAX, which was increased by 1% every 8 min) in the heat (34 C; 63% RH) and then performed a
second PVT. This task lasts 10 min and consists of pressing the mouse button as fast as possible in
response to visual stimuli. We assessed reaction time, false alarm and lapse of attention of the
volunteers. During the exercise trials, rectal temperature (TRET) and heart rate (HR) were
recorded. The fatiguing exercise in the heat and the acute supplementation with GLN or GLY did
not affect any of the parameters related to vigilance performance in PVT. Aside from this
observation, there were no differences in TRET and HR among the three experimental trials. Our
data indicate that prolonged exercise in the heat and acute supplementation with GLN did not
affect the performance on a vigilance task.
01.018 - TREINAMENTO FÍSICO AERÓBICO E RESISTIDO, PRÉVIOS AO INFARTO DO MIOCÁRDIO,
PREVINEM ALTERAÇÕES AUTONÔMICAS E INFLAMATÓRIAS EM RATOS
CATARINA DE ANDRADE BARBOZA1; NICOLLE MARTINS CARROZZI1; LUIZA MELANIE SILVA1; JULIANA
CRISTINA OLIVEIRA1; LEANDRO YANASE ROCHA1; DANIELE JARDIM FERIANI 1; MARCOS ELIAS
ABSSAMRA1; CRISTIANO MOSTARDA2; Mª CLAUDIA IRIGOYEN3; BRUNO RODRIGUES4
1.UNIVERSIDADE SAO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DO
MARANHÃO, SAO LUIS, MA, BRASIL; 3.INCOR-INSTITUTO DO CORACAO FMUSP, SAO PAULO, SP,
BRASIL; 4.UNICAMP UNIVERSIDADE DE CAMPINAS, SAO PAULO, SP, BRASIL.
O objetivo desse estudo foi comparar os efeitos do treinamento físico aeróbico (TA) e resistido
(TR), realizados previamente ao infarto do miocárdio (IM), em animais. Ratos Wistar foram
divididos em: controle (C), sedentário + IM (SI), TA + IM (TAI) e TR + IM (TRI). Após 2 meses de
treinamento/acompanhamento os animais foram submetidos ao IM e às avaliações autonômicas e
inflamatórias do ventrículo esquerdo em ratos. A área de IM não foi modificada pelo treinamento
prévio. A sensibilidade barorreflexa foi melhorada apenas no grupo TAI. Os grupos TAI (53±4 bpm)
e TRI (40±4 bpm) apresentaram semelhante redução do Tônus Simpático (TS) em comparação ao
grupo SI (117±6 bpm); no entanto, o Tônus Vagal (TV) foi aumentado somente nos animais TAI
(52±4 bpm), se comparados com os grupos SI (23±5 bpm) e TRI (21±3 bpm). Já a FC intrínseca,
reduzida no grupo SI, foi normalizada por ambos tipos de treinamento. As concentrações de
citocinas no ventrículo esquerdo IFN-γ, IL-6 e TNF-α, aumentadas no grupo SI (34,1±2; 143,7±12 e
35,3±2 pg/µg prot), foram reduzidas nos grupos TAI (20,1±3; 76,0±12 e 23,6±3 pg/µg prot) e TRI
(17,7±0,8; 68,7±7 e 20,6±3 pg/µg prot). Adicionalmente, a queda do TS foi correlacionada com a
redução de TNF-α (r=0,74; P=0,0001). Dessa forma, é possível inferir que, independentemente do
tipo, o treinamento físico pode ser uma ferramenta eficaz na prevenção das complicações
funcionais e teciduais oriundas da isquemia miocárdica.
01.019 - MODERATE HEAT STRESS INDUCES POST-EXERCISE HYPOTENSION AND INCREASES
PARASYMPATHETIC RESPONSE IN SHR RATS
MICHELE MACEDO MORAES; WASHINGTON PIRES; VALÉRIA ANDRADE PINTO; PATRÍCIA CONCEIÇÃO
ROCHA RABELO; SAMUEL PENNA WANNER; DANUSA DIAS SOARES
UFMG, BELO HORIZONTE, MG, BRASIL.
In spontaneously hypertensive rats (SHR), post-exercise hypotension is mediated by reductions in
peripheral resistance and sympathetic activity. As heat exposure increases sympathetic activity
and MAP in response to increases in core temperature (Tc), we investigated if a single bout of
exercise in warm (W) reduces MAP. 7 SHR (295±7g; 17,2±0,9wk) and 7 Wistar (WIS) (307,1±12,4g;
15±0,5wk) had a carotid catheter and a temperature sensor implanted. The rats were submitted
to a treadmill exercise (50% Vmax for 30 min, 5% inclination) in temperate (T) (23.5°C) and W
(30.3°C) environment. Pre and post exercise, the animals were maintained 2h at rest in the same
environment as during exercise. Spectral power components were evaluated to heart rate (HR)
and blood pressure (BP). MAP post-exercise reduced only for SHR (P<0.05) (T pre-exercise: 167 ±
4; post: 159 ± 4/ W pre: 154±7; post: 147±6mm Hg). In SHR, exercise in W reduced HR (pre:
320±12; post: 294±8bpm) and increased HF of HR (T pre: 4±1; post: 6±1/ W pre : 11±2; post: 11±1).
BP VLF component was reduced by the exercise in T (pre: 31±4/ post: 23±3). Tc was higher in W
during exercise for WIS and SHR, but not in pre or post-exercise (stabilized after 30 min of postexercise). Tail skin temperature was higher in W than in TN. In SHR, post-exercise hypotension
occurs even under moderate heat stress, which can be explained, at least in part, by increases in
parasympathetic response.
THEME 02 – RENAL PHYSIOLOGY
02.001 - CLONING OF NA+/K+-ATPASE SUBUNITS GENES FROM THE RODENT CERRADOMYS
GOYTACA
MARCOS FELIPE MARTINS GOMES; GEÓRGIA DA SILVA FELTRAN; CAROLINA AUGUSTA DE BARROS
SILVA; JACKSON DE SOUZA MENEZES; JOSÉ LUCIANO NEPOMUCENO SILVA; RODRIGO NUNES DA
FONSECA
UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, MACAÉ, RJ, BRASIL.
The Na+/K+-ATPase is a P type ATPase and acts as an electrogenic pump that promotes cellular
extrusion of 3 Na+ against the entry of 2 K+, using the energy released by the hydrolysis of ATP.
C.goytaca is a rodent species recently discovered in the Jurubatiba National Park Sand Bank (north
of Rio de Janeiro state), an environment where fresh water is usually scarce. The aim of this study
was the characterization, initially by cloning and sequencing, of α, β and γ subunit genes of renal
Na+/K+-ATPase from C.goytaca. After deep anesthesia and sedation (ketamine and xylazine),
renal excision was performed for total RNA extraction. 1 µg of total RNA was reversed transcribed
in cDNA and RT-PCR reactions were performed with primers sets designed based on multiple
alignments of the 3 Na+/K+-ATPase subunits genes from different species of rodents. RT-PCR
amplified products were ligated into pGEM-T-Easy vector and the ligation reaction was used to
transform competent E. coli. Colonies carrying vectors with inserts of expected size had their
plasmid DNA purified and sequenced. Sequences of fragments of approximately 1500, 950 and
210bp were obtained for subunits α, β, and γ genes, respectively. The gene fragment encoding
the first half of α subunit shares 99% identity with the orthologue from Rattus norvegicus. Other
sequences are presently under analysis. Conclude that the C.goytaca expresses the 3 Na+/K+ATPase subunits in the kidney and that these genes shares high similarity with other rodents
orthologues.
02.002 - RENAL DAMAGE INDUCED BY ADRIAMYCIN: INFLUENCE OF PHYSICAL PRECONDITIONING
CAMILA DE MATTOS FALEIROS; HELOÍSA D COLETTA FRANSCESCATO; MARCELO PAPOTI; LUCAS
CHAVES; CLEONICE GIOVANINI; ROBERTO SILVA COSTA; TEREZILA MACHADO COIMBRA
UNIVERSIDADE DE SÃO PAULO, RIBEIRAO PRETO, SP, BRASIL.
Adriamycin (ADR) nephropathy is an experimental model for evaluation of the mechanisms
involved in progressive renal disease. A single dose of ADR in rats induces progressive proteinuria
and renal structural changes that lead to glomerular and tubulointerstitial lesions. The aim of this
study was to evaluate the influence of physical preconditioning in the renal structure and function
in rats treated with ADR. Male Wistar rats (n=27) previously submitted or not to physical training
in a treadmill running (4 weeks) were injected with ADR (2.5 mg/kg) (PhT+ADR and ADR groups)
or saline (PhT+SAL and SAL groups). Urine samples were collected 7 and 30 days after injections
to quantify albumin excretion, and blood samples 60 days after treatment to evaluate plasma
creatinine levels (Pcreat). The kidneys were removed for morphometric and immunohistochemical
analysis. There was a progressive rise in albuminuria in AD-injected rats on the 7th and 30th days,
but with less intensity in PhT+ADR (p<0.05). The Pcreat (mg%) increased in ADR group (1.43±0.45)
but not in the others groups (PhT+ADR=0.78±0.36; PhT+SAL=0.83±0.12; SAL= 0.90±0.04). The
increased vimentin expression and relative interstitial area in renal cortex in ADR group were
attenuated in PhT+ADR group (p<0.05). Physical preconditioning can attenuate the renal lesions
provoked by ADR. This effect was associated with attenuation of albuminuria and relative
interstitial area increases and with maintenance of Pcreat and cortical expression of vimentin.
02.003 - RESTRIÇÃO PROTEICA DURANTE A PUBERDADE INDUZ AUMENTO DA PRESSÃO ARTERIAL
A LONGO TERMO SEM AFETAR FUNÇÃO RENAL
LETICIA DINIZ CREPALDI
UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL.
A síndrome cardiometabólica tem alcançado proporções epidêmicas. Estudos demonstram que a
desnutrição, em fases críticas do desenvolvimento, é um fator importante para o desenvolvimento
dessa síndrome na vida adulta. Este trabalho teve como objetivo analisar parâmetros da função
renal e pressão arterial de ratos Wistar que receberam dieta hipoproteica durante a puberdade.
Os animais foram separados em dois grupos: G-NP (dieta normoproteica - n= 9) e G-HP (dieta
hipoproteica - 4% de proteínas - n= 9). A dieta foi administrada dos 30 aos 60 dias de vida. Aos
120 dias, foi feito registro da pressão arterial, coleta de sangue, medida da ingestão hídrica e
urina de 24 h, análises da proteinúria e da taxa de filtração glomerular (TFG). Foi observado
redução significativa no peso corporal (G-NP=355,38 ± 21,77 e G-HP = 329,65 ± 21,10g - p=0,028),
no comprimento nasoanal (G-NP = 23,69 ± 0,45 e G-HP =23,04 ± 0,66cm - p=0,025) e aumento
significativo na pressão arterial média (G-NP = 95,7 ± 7,2 e G-HP = 108,3 ±8,1mmHg - p=0,01) no
G-HP. No entanto, não houve alteração nem na TFG (G-NP=0,4 ± 0,10 e G-HP=0,4 ±
0,06mL/min/100g - p=0,89) nem na proteinúria (G-NP =7,04 ± 2,03 e G-HP=7,67 ± 1,76mg/24hrs p=0,48). Concluindo, animais que receberam dieta hipoproteica, durante a puberdade,
apresentaram aumento da pressão arterial na vida adulta sem alteração na taxa de filtração
glomerular e proteinúria.
02.004 - RENAL EFFECTS OF DIABETES MELLITUS INDUCED DURING PREGNANCY AND
MAINTAINED IN THE POSTPARTUM PERIOD IN WISTAR RATS
NATHANE FRANÇA SILVA; NATANY GARCIA REIS; PÂMELLA FRANCIS SANTOS; ANA PAULA COELHO
BALBI
FEDERAL UNIVERSITY OF UBERLÂNDIA (UFU), UBERLÂNDIA, MG, BRASIL.
4
Objective: Evaluate the effects of diabetes mellitus (DM) induced in Wistar rats during pregnancy
and maintained by 50 days in the postpartum period on structural and renal function. Material
and methods: There were four groups (G): G1 (non-pregnant controls rats), G2 (non-pregnant
diabetic rats), G3 (control mothers) and G4 (diabetic mothers). Results: systolic blood pressure
were not different among the groups, but there was a tendency to reduction in glomerular
filtration rate from G4 when compared to the other G. Morphometric analysis showed a reduction
of total renal sectional area from G4 and an increase in G3. The glomerular area and the capsular
space did not differ between G studied, but there was an increase in glomerular tuft area in G3
and G4. G2 and G4 showed higher percentage of collagen cortical. Regarding PCNA
immunoreactivity, G3 showed higher number of glomerular proliferating cells while in G4 it was
lower, when compared with their respective controls. Further, G4 showed higher number of
proliferating cells in the tubulointerstitial (TBI) compartment. Glomerular and TBI expression of
α-SMA were increased in G4 compared to other G. Immunoreaction for glomerular p-p38
expression showed a reduction in G4, while p-JNK expression was higher in both the glomeruli and
TBI compartment in G4 compared to other G. Conclusion: DM resulted in renal impairment on the
postpartum period and these alterations may be related to changes in the expression of MAPK.
02.005 - ACUTE MICROCISTIN-LR EXPOSURE INDUCE CHANGES IN MICE RENAL FUNCTION AND
PODOCIN MRNA EXPRESSION
GEÓRGIA DA SILVA FELTRAN; RODRIGO NUNES DA FONSECA; NATALIA FEITOSA MARTINS; RAQUEL
MORAES SOARES; SANDRA MARIA FELICIANO DE OLIVEIRA E AZEVEDO; JACKSON DE SOUZA MENEZES
., ., RJ, BRASIL.
Cyanobacterias are photosynthetic prokaryotes potentially producing toxins, which can cause
severe physiological damage. The aim this work was analyze changes in renal function of mice
intoxicated by microcystin-LR. C57BL6 mice were divided into control (CTRL) and 35 ug/kg of
microcystin-LR IP (MYCST35). After deep anesthesia and sedation, blood sample was collected (for
plasma isolation) and the organs was excised. The renal function was assed using clearance
methodology. The organs index were calculated by the following ratio: organ weight/body weight.
The kidneys were excised for RNA extraction, using TRIZOL reagent, and cDNA was synthesized.
The podocin relative mRNA expression was determined by qRT-PCR technique. Statistical analyzes
were performed using the t test and the differences were considered significant when p<0.05. In
MCYST35 group it was observed higher glucose fractional excretion (%) and clearance (ml/min/kg)
(0.068±0.0086 and 0.0043±0.0079) and lower GFR (ml/min/kg) (0.0059±0.0015) compared to
control (0.0230.011, 0.0000160.0000039 and 0.0170.0042, n=4-11). The podocin relative
expression was lower in MCYST35 (0.25 ± 0.05) compared to control (1.00.0, n=3). The right
kidney, liver and lung index was higher in MCYST35 (0.0068±0.00028, 0.055±0.0012 and
0.0110.0013) compared to control (0.00590.00015, 0.0470.0040 and 0.00690.00015, n=6). In
this work it was observed that acute microcistin-LR intoxication induce changes in renal function
and organs index.
02.006 - EVALUATION OF REPRODUCTIVE BEHAVIOR AND GROWTH OF RODENT CERRADOMYS
GOYTACA
CAROLINA AUGUSTA BARROS; RODRIGO NUNES DA FONSECA; MARCOS FELIPE MARTINS GOMES;
JOSUE MARCELO ALMEIDA; JACKSON DE SOUZA MENEZES
UFRJ, MACAÉ, RJ, BRASIL.
Cerradomys goytaca is the lasted mammal discovered in the Sandbank Jurubatiba National Park
(North of Rio de Janeiro State). The reproduction of this rodent in vivarium is the initial steps to
evaluate physiological adaptations to survive in sandbank environment. The animals were fed with
regular rat/mouse chow and water ad libitum. They were kept in daily 12-hour cycle of light/dark
and temperature of 23 ± 1 ° C and relative humidity of 60%. The offspring were monitored weekly
from seventh to twenty first week after delivery by measuring the weight (g), naso-anal and caudal
length (cm). The gestational age was 28 to 32 weeks and weaning occurred in the sixth week after
delivery. The mean number of offspring was 4.3±0.3. The rate of newborn mortality was about
20%. One week after weaning, the weight (g) of females was 42.0±2.7 and males was 34.0±1.0.
The males gain of weight (g) were 41.0±6.0 and females were 38.6±3,1 (p>0.05) in 14 weeks after
weaning. The body males longitudinal growth and caudal growth (cm in 14 weeks after weaning)
were respectively 7.35±1.11, 3.55±0.45 and females were, respectively 6.38 ± 0.72, 4.18±0.52
(p>0.05). It was not observed a significant correlation between room humidity / pregnancy and
female age / maternal cannibalism. It was observed a moderate negative correlation between
room temperature and pregnancy. Cerradomys goytaca rodents are able to reproduce and survive
in animal vivarium.
02.007 - EVALUATION OF THE H2O TRANSPORT MEDIATED BY HUMAN UREA TRANSPORTERS A1,
A2, A3 AND B HETEROLOGOUSLY EXPRESSED IN RANA CATESBEIANA OOCYTES
JESSICA KABUTOMORI
UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL.
Urea contributes to the inner medullary osmolality gradient, important for regulating H 2O
excretion under the influence of antidiuretic hormone. Mammalian urea transporters (UTs,SLC14
family) facilitate diffusion of urea across the plasma membrane:UTA1 is expressed on the apical
membrane and UTA3 on the basolateral membrane in the inner medulla, UTA2 in the thin
descending limb in the outer and inner medulla, and UTB in the descending vasa recta. Although
it is clear that human (h) UTs plays a critical role in the urinary concentrating mechanisms, there
is some debate as to whether hUTs also facilitate H 2O transport in the kidney. Previously, it was
shown that hUT-B can function as a H2O channel. This study explores the osmotic water
permeability (Pf) of hUTA1, A2, A3, and B heterologously expressed in Rana catesbeiana oocytes
using video microscopy to monitor cell swelling as we reduce the extracellular osmolality. Before
functionally characterizing the hUTs, we performed western blotting experiments of biotinylated
samples to confirm that the UTs were inserted into the plasma membrane. Our Pf values for UTA2
(0.0039+/-0.001,n=18), UTA3 (0.0035+/-0.0008,n=15) and UTB (0.0022+/-0.0007,n=8) expressing
oocytes were all significantly greater than that of control H2O-injected (0.0005+/-0.00006,n=24)
oocytes, while UTA1 expression had no effect on the oocyte P f. Thus, our data confirm that UTB
conducts H2O, similar to the observation seen by others using Xenopus oocytes, and indicate for
the first time that UTA2 and UTA3-expressing Rana oocytes have significant H2O permeability.
02.008 - EFFECT OF PHYSICAL TRAINING IN THE EVOLUTION OF ACUTE RENAL INJURY IN RATS:
ROLE OF ENDOTHELIAL CELLS
HELOISA DELLA COLETTA FRANCESCATO; CAMILA DE MATTOS FALEIROS; MARCELO PAPOTI; LUCAS
CHAVES; ROBERTO SILVA COSTA; TEREZILA MACHADO COIMBRA
UNIVERSITY OF SAO PAULO, RIBEIRAO PRETO, SP, BRASIL.
Endothelial dysfunction occurs in acute renal failure, with increased expression of antiangiogenic
factors and loss of angiogenic factors such as vascular endothelial growth factor (VEGF),
contributing to the establishment and progression of renal injury. This study evaluated the effect
of physical training in acute kidney injury (administration of cisplatin, CP) on the regeneration of
the endothelium as well in the inflammatory process. Male Wistar rats (n=12) previously submitted
or not to physical training in a treadmill running (4 weeks) were injected with CP (5 mg/kg)
(PhT+CP and CP groups, respectively) or saline (Control group). Blood and urine samples were
collected 5 days after injections to evaluate plasma creatinine levels (Pcreat;mg%) and sodium
fractional excretion (FENa+;%), and kidneys were removed for immunohistochemical studies
(macrophages and VEGF expressions). CP-treated rats presented increased Pcreat, FENa+, and
number of macrophages (ED1+ cells/100mm2) in the renal outer medulla, which were attenuated
in PhT+CP group. There was a greater immunostaining (evaluated by score) for VEGF in renal outer
medulla in PhT+CP group compared to C and CP groups. We found a negative correlation between
macrophage infiltration and VEGF expression (R=0.786;p=0.0015). The effect of physical training
in renal lesion provoked by CP was associated with less inflammatory process and increased VEGF
expression, contributing to decrease of renal damage.
THEME 03 – NEUROPHYSIOLOGY
03.001 - MUSCIMOL INJECTED INTO THE LATERAL PARABRACHIAL NUCLEUS INCREASES
POTASSIUM CHLORIDE INTAKE IN THE RAT
JOAO CARLOS CALLERA1; LAURIVAL ANTONIO DE LUCA JR2; JOSÉ VANDERLEI MENANI2
1.DEPT. OF BASIC SCIENCES, SCHOOL OF DENTISTRY, UNIVERSIDADE ESTADUAL PAULISTA (UNESP),
ARACATUBA, SP, BRASIL; 2.DEPT. OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY,
UNESP, ARARAQUARA, SP, BRASIL.
Muscimol, GABAA receptor agonist, into the lateral parabrachial nucleus (LPBN) results in
ingestion of water and different mineral solutions containing sodium (0.3 M NaCl or 0.3 M
NaHCO3). In the present study, we investigated if muscimol into the LPBN would modify the
ingestion of other mineral solution (potassium chloride, KCl) in normohydrated, in cell-dehydrated
rats by an intragastric load of NaCl (IG 2 M NaCl) or in rats with sodium depletion (treatment with
the diuretic furosemide subcutaneously + sodium deficient food for 24 h). Deionized water and
0.3 M KCl intake was measured at every 30 min during 240 min, starting 15 min after injections of
muscimol (0.5 nmol/0.2 ml) or saline into the LPBN. Muscimol into the LPBN increased 0.3 M KCl
intake in normohydrated rats (5.1 ± 1.4, vs. saline: 0.1 ± 0.1 ml/240 min), in rats treated with IG
2 M NaCl (20.6 ± 2.7, vs. saline: 1.0 ± 0.6 ml/240 min) and in sodium-depleted rats (8.3 ± 2.9, vs.
saline: 0.5 ± 0.1 ml/240 min). Muscimol into the LPBN also increased water intake in
normohydrated rats and in sodium-depleted rats, however, muscimol reduced water intake in the
first 150 min of test in rats treated with IG 2 M NaCl. These data show that besides sodium and
water intake, muscimol also increase 0.3 M KCl intake in normohydrated, cell-dehydrated and
sodium depleted rats, suggesting that the activation of GABAA receptors in the LPBN releases the
ingestion of different mineral solutions.
03.002 - REABILITAÇÃO NEUROMUSCULAR ATRAVÉS DE MUSICOTERAPIA
NATHALYA DE CARVALHO AVELINO1; MARLY CHAGAS OLIVEIRA PINTO2; OVIDIU CONSTANTIN
BALTATU1; LUCIANA APARECIDA CAMPOS1
1.CAMILO CASTELO BRANCO UNIVERSITY (UNICASTELO), SAO JOSE DOS CAMPOS, SP, BRASIL;
2.2CONSERVATÓRIO BRASILEIRO DE MÚSICA - CENTRO UNIVERSITÁRIO, RIO DE JANEIRO, RJ,
BRASIL.
Introdução: Reabilitação neuromuscular com exercícios específicos com musicoterapia induzem
treinamento de feedback multissensorial através da ativação do acoplamento auditório sensório
motor. O objetivo deste estudo foi investigar os efeitos da musicoterapia na reabilitação
neuromuscular de pacientes com trauma musculoesquelético no pós-operatório.
Material e Métodos: Pacientes pós-operatório foram divididos em um grupo controle apenas com
fisioterapia e um grupo submetido a fisioterapia e musicoterapia combinados com exercícios
específicos. Força, amplitude de movimento de extensão e flexão de punho foram avaliadas por
dinamômetro Kratos, e movimento angular por goniômetro durante 5 dias de reabilitação.
Resultados: A força aumentou significativamente entre os dias 1 e 5 (13.0 ± 2.6 vs 23.0 ± 2.6 kgf,
respectivamente) e dias 2 e 5 (16.0 ± 3.1 vs 23.0 ± 2.6 kgf, respectivamente) somente no grupo
com musicoterapia.
A amplitude de flexão aumentou significativamente a partir do segundo dia de musicoterapia
(41.0° ± 5.0), enquanto apenas no dia 5 (34.5° ± 8.2) no grupo controle. No final da reabilitação
o grupo com musicoterapia havia superado o grupo controle quanto a amplitude de flexão (51° ±
9.2 vs 34.5° ± 8.2).
A amplitude de extensão aumentou de forma significativa a partir do quarto dia de musicoterapia
(42.0° ± 7.8), enquanto que no grupo controle este aumento ocorreu apenas no quinto dia (64.5°
± 7.8).
Conclusoes: O treinamento motor com suporte da música pode representar uma estratégia
terapêutica eficaz para a reabilitação neuromuscular pós-operatória.
03.003 - TREATMENT WITH L-DOPA DOES NOT RESTORE RESPIRATORY IMPAIRMENT IN AN
EXPERIMENTAL MODEL OF PARKINSON'S DISEASE
ARIADINY DE LIMA CAETANO; MARINA TUPPY; THIAGO S MOREIRA; ANA CAROLINA T TAKAKURA
UNIVERSIDADE DE SAO PAULO, SAO PAULO, SP, BRASIL.
Parkinson's disease (PD) is a neurodegenerative disease characterized by the loss of dopaminergic
neurons in the substantia nigra (SN). The symptoms are: tremor at rest, muscular rigidity,
bradykinesia, hypokinesia and postural disturbance. Aside from the classic symptoms, there are
secondary symptoms associated with PD, like respiratory deficiencies. The classic pharmacological
treatment for PD is the use of L-3,4-dihydroxyphenylalanine (L-DOPA). Recently our laboratory
showed a respiratory failure in a 6-hydroxydopamine (6-OHDA) animal model of PD. The aim of
this study was to verify whether L-DOPA treatment would be able to restore breathing in our PD
model. Male Wistar rats (250-300g, N=5) received bilateral 6-OHDA (12µg/µL) injection in the
striatum. Twenty days after 6-OHDA injection, treatment of saline or L-DOPA (Prolopa® HBS,
100mg/benserazide hydrochloride 25mg, ip) were performed for 40 days. The functional
respiratory responses were measured by whole-body plethysmography and immunohistochemistry
was performed to evaluate the SN lesion. Bilateral injection of 6-OHDA in the striatum elicited
reduction of 80% of catecholaminergic neurons in the SN. The L-DOPA treatment did not restore
the reduction in respiratory frequency (88±11; vs. saline: 92±11bpm) produced by 6-OHDA in the
striatum. This study shows that treatment with L- DOPA is not able to restore the breathing deficits
observed in the PD model induced by bilateral injection of 6-OHDA in the striatum
03.004 - MINERAL PREFERENCE IN RATS TREATED WITH INJECTIONS OF MUSCIMOL INTO THE
LATERAL PARABRACHIAL NUCLEUS
JOAO CARLOS CALLERA1; LAURIVAL ANTONIO DE LUCA JR2; JOSÉ VANDERLEI MENANI2
1.DEPT. OF BASIC SCIENCES, SCHOOL OF DENTISTRY, UNIVERSIDADE ESTADUAL PAULISTA (UNESP),
ARACATUBA, SP, BRASIL; 2.DEPT, OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY,
UNESP, ARARAQUARA, SP, BRASIL.
It was previously demonstrated that rats treated with bilateral injections of muscimol, GABA A
receptor agonist, into the lateral parabrachial nucleus (LPBN) ingest water and different mineral
solutions containing sodium (0.3 M NaCl or 0.3 M NaHCO 3) in a two-bottle choice test. In the
present study, we investigated mineral preference after bilateral injections of muscimol into the
LPBN in normohydrated rats or in hyperosmotic cell-dehydrated rats treated with intragastric (IG)
load of 2 M NaCl. Male adult Wistar rats (n=6) with bilateral stainless steel guide-cannulas
implanted into the LPBN had deionized water and four mineral solutions at 0.3 M concentration
(NaCl, NaHCO3, CaCl2 and KCl) simultaneously available for consumption in a five-bottle test. The
ingestion was measured at every 30 min during 240 min, starting 15 min after the injections into
the LPBN. Normohydrated rats treated with bilateral injections of muscimol (0.5 nmol/0.2 µl) into
the LPBN ingested mainly 0.3 M NaCl and a less amount of NaHCO 3, but not CaCl2, KCl or water.
Cell-dehydrated rats treated with IG 2 M NaCl combined with injections of muscimol into the LPBN
ingested more 0.3 M NaCl than all other fluids. These data suggest a clear preference for sodium
intake when the inhibitory mechanisms are blocked by GABA A receptor activation with muscimol
injections into the LPBN in rats.
03.005 - A6 NORADRENERGIC NEURONS ARE RECRUITED IN A RAT MODEL OF PARKINSON’S
DISEASE
LUIZ MARCELO OLIVEIRA; MARINA TUPPY; THIAGO S MOREIRA; ANA CAROLINA T TAKAKURA
UNIVERSITY OF SÃO PAULO, SAO PAULO, SP, BRASIL.
Previous study has already demonstrated that in 6-hydroxydopamine (6-OHDA)-model of
Parkinson´s disease (PD) there is a reduction in the number of Phox2b neurons in the
retrotrapezoid nucleus (RTN) and a decrease in the respiratory response to hypercapnia 40 days
after PD-induced. This functional deficiency is restored 60 days after 6-OHDA injection and here
we tested the hypothesis that A6 noradrenergic cells could be a candidate to restore this
deficiency in this model. Minute Ventilation (VE) in response to hypercapnia (7% CO2) was assessed
one day before and 60 days after bilateral 6-OHDA (24 µg/µl) or vehicle injections in the striatum
(CPu) and in the A6 in Male Wistar rats (CEUA: 14, p.15, b.03/2014). Bilateral injections of 6OHDA decreased cathecolaminergic neurons by 86% and 83% in the substancia nigra (SN) and A6,
respectively. In animals with lesion in the SN and A6 (N = 5/group) there is a reduction in the
ventilatory response to hypercapnia (785±18 vs vehicle: 1417±177 ml/kg/min). In another group
of rats after 60 days of injections of 6-OHDA in the CPu (N = 4/ group), the rats were exposed to
hypercapnia or normocapnia for 3 hours and there was a reduction in the number of hypercapniainduced-Fos-ir cells in the RTN region (8.5 ±0.9, vs. vehicle 78±3). However, in PD rats,
hypercapnia was able to induce Fos-ir cells in the A6 region (94±22 vs. vehicle 1±1). Our data
5
suggest that A6 noradrenergic neurons can be a candidate to assume the chemoreceptor function
in a model of PD.
03.006 - EFFECTS OF CHRONIC UNPREDICTABLE STRESS ON DEPRESSIVE BEHAVIOR IN RATS
WITH PREMATURE OVARIAN FAILURE
JORDANA LAIS DE ROCCO1; FLAVIANO LORENZON1; TAMIRES GREGORIO1; BRUNA BARCELOS DE
SIMAS1; JANETE APARECIDA ANSELMO-FRANCI2; CILENE LINO OLIVEIRA1; DOMITILA AUGUSTA
HUBER1; FERNANDA BARBOSA LIMA CHRISTIAN1
1.UNIVERSIDADE FEDERAL DE SANTA CATARINA, FLORIANOPOLIS, SC, BRASIL; 2.UNIVERSIDADE DE
SAO PAULO, RIBEIRA PRETO, SP, BRASIL.
Stress can contribute to the higher incidence of affective disorders in women during
perimenopause. We have investigated the impact of chronic unpredictable stress (CUS) on
behavior in the forced swim test (FST) in rats with premature ovarian failure (POF). Female rats
(28 days old) were treated with 4-vinylcyclohexane diepoxide (VCD 160 mg/kg, ip) or vehicle (CTL)
for 15 consecutive days. After 70 days, rats were submitted to CUS for 10 days, including 4 sections
of FST. Proactive (swimming, diving and climbing) and passive (immobility) behaviors were
evaluated. VCD group increased immobility (Day 2: 98±62; Day 4: 144±76) and decreased climbing
(Day 2: 169±65; Day 4: 115±66) on the last day of FST. CTL rats increased duration (Day 1: 20±16;
Day 3: 54±25) and frequency (Day 1: 2.5±1.3; Day 3: 8.5±3) of swimming on day 3 of FST. VCD
group presented higher latency for swimming (Day 1: 54±67; Day 2: 149±92) and diving (Day 1:
94±117; Day 4: 274±76) in day 4 of FST. Both duration (Day 4 VCD: 0.43±14; Day 4 CTL: 7.4±8,)
and frequency (Day 4 VCD: 0.22±0.7; Day 4 CTL: 3.8±4) of diving were significantly lower in VCD
rats compared to CTL. Latency to dive was higher in VCD compared to CTL (Day 4 VCD: 274±76
sec; Day 4 CTL: 142±101sec). The results indicate that POF leads rats under chronic unpredictable
stress to develop passive strategies to cope with stress in the FST. Hence, during the progression
of POF, stress may reinforce depressive disorders.
03.007 - WATER DEPRIVATION-INDUCED SODIUM APPETITE IS ENHANCED IN RENOVASCULAR
HYPERTENSIVE RATS
CAMILA FERREIRA RONCARI; RAFAELA MOREIRA BARBOSA; LAURIVAL ANTONIO DE LUCA JR; JOSÉ
VANDERLEI MENANI; EDUARDO COLOMBARI; DEBORA SIMÕES ALMEIDA COLOMBARI
DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, DENTISTRY SCHOOL, UNESP, ARARAQUARA, SP,
BRASIL.
Sodium appetite dependent on the activation of the renin angiotensin system (RAS) is induced
after water deprivation (WD) followed by water intake (partial rehydration - PR). Rats with 2kidney, 1-clip (2K1C) hypertension present an overactive RAS. In the present study, we evaluated
0.3 M NaCl and water ingestion in normotensive and 2K1C hypertensive rats submitted to the WDPR protocol. Male Holtzman rats (150 – 180 g, n = 5/group) received a silver clip around the left
renal artery (2K1C hypertensive rats) or no clip around the artery (sham rats). After six weeks,
2K1C hypertensive and sham rats were submitted to 24 h of WD, before the access to only water
to ingest for 2 h (PR). At the end of this period, 0.3 M NaCl was also available and the intake of
both 0.3 M NaCl and water was recorded for an additional 2 h (salt appetite test). After WD, 2K1C
hypertensive and sham rats ingested similar amount of water intake during the PR (15.9 ± 4.1, vs.
sham: 14.8 ± 1.2 ml/2 h). Compared to sham rats, in the salt appetite test, 2K1C hypertensive
rats ingested more 0.3 M NaCl (8.0 ± 1.2, vs. sham: 3.4 ± 1.2 ml/2 h) and water (10.3 ± 4.4, vs.
sham: 2.3 ± 0.9 ml/2 h). The results show that 2K1C hypertensive rats have a stronger sodium
appetite than normotensive rats when submitted to the WD-PR protocol, probably due to the
overactivity of RAS in these animals.
03.008 - INIBIÇÃO DA ISOFORMA NEURONAL DA NOS NÃO ALTERA HORMÕNIOS
NEUROHIPOFISÁRIOS DE RATOS SUBMETIDOS A SOBRECARGA SALINA
FABIANA LUCIO-OLIVEIRA1; JULIANA BEZERRA MEDEIROS DE LIMA2; FERNANDA MARIA VEANHOLI
VECHIATO2; RICARDO COLETTI2; TATIANE VILHENA FRANCO2; LUCILA LEICO KAGOHARA ELIAS2;
JOSÉ ANTUNES-RODRIGUES2
1.INSTITUTO FEDERAL DO SUL DE MINAS GERAIS, MUZAMBINHO, MG, BRASIL; 2.FMRP, RIBEIRAO
PRETO, SP, BRASIL.
O óxido nítrico tem sido sugerido exercer um papel proeminente na regulação do eixo hipotálamohipófise. O objetivo deste estudo foi avaliar o efeito do N-propil-L- arginina (NPLA), um inibidor
seletivo da NOS neuronal, sobre os níveis plasmáticos de AVP e OT em ratos submetidos à
sobrecarga de sal. Ratos wistar machos implantados ICV permaneceram em SS por 96 horas. Uma
hora e 30 minutos antes de decapitação, os ratos foram tratados com uma injecção ICV de NPLA
ou veículo. Uma hora após a injeção icv, água e solução NaCl 0,3 M foram novamente oferecida.
Após esse período, os animais foram decapitados, seu sangue foi coletado para determinação da
osmolalidade e para dosagens hormonais. O cérebro foi removido e o HMB foi coletado para a
dosagem da atividade da NOS. Os nossos dados mostraram, o aumento da osmolalidade de ratos
que foram submetidas a SS. O aumento da osmolalidade foi seguido por um aumento na atividade
NOS no HMB de ratos com SS. Entretanto, os animais submetidos a SS tratados com NPLA tiveram
uma diminuição na atividade NOS cerca de 44% quando comparado aos animais tratados com
veículo. Houve um aumento significativo de AVP e OT em animais submetidos à SS, mas nenhuma
diferença foi observada no tratamento com NPLA. Estes resultados indicam que a modulação de
NO sobre a secreção de AVP e OT observado em vários estudos provavelmente não é realizada
através da isoforma neuronal de NOS, ou que o nível de inibição de NOS não foi eficaz na
modulação da resposta desses hormônios durante estímulos osmóticos como SS.
03.009 - HYPOXIA ACTIVATES BRAINSTEM AND HYPOTHALAMIC NEURONS THAT EXPRESS LEPTIN
RECEPTORS
MIRIAN BASSI1; JOSÉ VANDERLEI MENANI1; DEBORA SIMÕES ALMEIDA COLOMBARI1; DANIEL
BRESEGHELLO ZOCCAL1; JOSE DONATO JR2; EDUARDO COLOMBARI 1
1.UNESP, ARARAQUARA, SP, BRASIL; 2.USP, SAO PAULO, SP, BRASIL.
Leptin facilitates chemoreflex ventilatory responses by mechanisms not completely clear yet.
Therefore, in this study, we evaluated c-Fos expression induced by hypoxia in brainstem and
hypothalamic neurons that express leptin receptors (LRs) to identify possible sites of leptin action
to modulate breathing. Male LepR-reporter mouse (n=5) that allow to visualize LR-expressing cells
with the tdTomato fluorescent protein were used. Cells with c-Fos expression were identified
using immunohistochemistry procedures after the exposure of mice to hypoxia (7% of O2 in
breathing air) during 90 min followed by additional 90 min in normoxic air. The LRs and c-Fos
expression was quantified in one coronal section (30 µm) of each analyzed nucleus. LRs were
predominant, among other areas, in the commissural nucleus of the solitary tract (cNTS: 50±5
cells/section), lateral parabrachial nucleus (LPBN: 119±6 cells/section) and arcuate nucleus of
hypothalamus (ARC: 139±20 cells/section). Hypoxia induced c-Fos expression co-localized with
LRs mostly in the cNTS (49%) and ARC cells (85%). The LPBN showed low percentage of cells coexpressing c-Fos/LRs (4%). The locus coeruleus, an important area for the ventilatory regulation,
is also activated by hypoxia (c-Fos: 63±22 cells/section), however, no labelling for LRs were
detected in this area. These data show that cNTS and ARC are main areas with LRs cells activated
by hypoxia and, therefore, possible sites of action of leptin to modulate the ventilatory responses
to hypoxia.
03.010 - INTERNAL BODY TEMPERATURE SIGNALS TO BIOLOGICAL CLOCKS THROUGH TRPV1
CHANNELS
NAYARA ABREU COELHO HORTA1; FREDERICO SANDER MANSUR MACHADO1; THAIS SANTANA ROCHA
CARDOSO1; MAURO SÉRGIO BATISTA SILVA1; CÂNDIDO CELSO COIMBRA1; SAMUEL PENNA WANNER1;
ANA MARIA DE LAURO CASTRUCCI2; MARISTELA DE OLIVEIRA POLETINI1
1.FEDERAL UNIVERSITY OF MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSITY OF SÃO
PAULO, SÃO PAULO, SP, BRASIL.
Our group has been studying the role of TRPV1 channels in signaling temperature to peripheral
clocks. In a previous study, we observed that acute blockade of TRPV1 channels alters the
expression of clock genes in peripheral tissues. In this study, we investigated whether
desensitization TRPV1 disrupts the molecular clock oscillation in peripheral tissues. Rats received
an injection of 20 µg/kg body weight ip of resiniferatoxin (RTX) and after five days were
euthanized at zeitgeber (ZT) 0 (time of light on), ZT6, ZT12 and ZT18. The total RNA was extracted
from the liver, adrenal and microdissections of the suprachiasmatic nucleus (SCN). Relative
analyses of clock genes, rPer1 and rBmal1 mRNA, were performed by qPCR. The desensitization
abolished the adrenal circadian profiles of rPer1 and rBmal1 expression. This was also observed
in liver only for rPer1 expression, although rBmal1 was increased at ZT0. Intriguingly, in the SCN
(the central clock), TRPV1 desensitization altered the circadian profile of rBmal1 expression
regardless of changes on rPer1 circadian expression. We previously observed that acute blockade
of TRPV1 channels decreases corticosterone plasma levels, this may be a secondary signal from
peripheral clocks to the central pacemaker, since RTX induced desensitization is restricted to the
abdominal cavity. Thus, TRPV1 may mediate the temperature-induced rhythm of peripheral
clocks.
03.011 - ROLE OF NITRIC OXIDE OF THE DORSOLATERAL PERIAQUEDUCTAL GRAY MATTER IN
RESTRAINT-INDUCED FEVER IN RATS
VICTOR MARTINS; CAROLINE CRISTINA SILVA; LUCIANE HELENA GARGAGLIONI BATALHAO; KÊNIA
CARDOSO BÍCEGO
COLLEGE OF AGRICULTURAL AND VETERINARIAN SCIENCES - SAO PAULO STATE UNIVERSITY,
JABOTICABAL, SP, BRASIL.
Nitric oxide (NO) acts in different brain regions as a pyrogenic or antipyretic agent during systemic
inflammation induced by endotoxin. There is evidence of its role in restraint-induced fever but it
is unknown its site of action in the brain. The periaqueductal gray (PAG) is a possible site of action
for NO because it is involved in thermoregulation and stress responses (fear and defensive
behavior). The dorsolateral PAG (PAGdl) expresses large amount of the enzyme NO synthase (NOS).
Thus, the aim of this study was to test the hypothesis that NO acts in the PAGdl as an antipyretic
agent in restraint stress-induced fever in rats. To this end, it was verified the effect of
microinjection of non-selective NOS inhibitor, L-NMMA, in PAGdl on body temperature (Tb) and
on heat loss index (HLI) of Wistar rats exposed or not to physical restraint. No difference was
observed in Tc of control rats (saline: 37.4 +/- 0.1; L-NMMA: 37.5 +/- 0.1). During physical
restraint, there was an increase in Tb of all groups, but the response was more pronounced after
L-NMMA injection in PAGdl. The HLI similarly increased in all groups after restraint stress. These
preliminary results indicate that the NO seems to act as antipyretic molecule in PAGdl during
fever induced by restraint stress, without affecting the heat dissipation mechanism.
03.012 - ROLE OF MU AND KAPPA OPIOID RECEPTORS OF THE PERIAQUEDUCTAL GRAY IN
RESTRAINT-INDUCED FEVER
CAROLINE CRISTINA SILVA; VICTOR MARTINS; LUCIANE HELENA GARGAGLIONI BATALHAO; KÊNIA
CARDOSO BÍCEGO
COLLEGE OF AGRICULTURAL AND VETERINARIAN SCIENCES, SÃO PAULO STATE UNIVERSITY,
JABOTICABAL, SP, BRASIL.
The endogenous opioids are involved in analgesia, thermoregulation and physiological responses
to various stressful stimuli such as infection, psychological stress and hypoxia. The mu and kappa
receptors in the hypothalamus play a role in endotoxin-induced fever and hypoxia-induced
anapyrexia (opposite response to fever), respectively. In addition, periaqueductal gray (PAG),
which express both mu and kappa receptors, is involved in defense and thermoregulatory
responses. Thus, our hypothesis is that mu and kappa opioid receptors in the PAG modulate the
restraint-induced fever in rats by activating and inhibiting this response, respectively. To test this
hypothesis, it was verified the effect of intra-PAG injection of mu antagonist (CTAP) and kappa
antagonist (nor-BNI) on body temperature (Tb; datalogger implanted in the abdominal cavity) of
Wistar male rats submitted or not to restraint stress. The Tc of control animals did not differ
between saline (37.35 ± 0.097), nor-BNI (37.75 ± 0.148) and CTAP (37.35 ± 0.092) groups. Restraint
stress increased the Tb of nor-BNI and saline groups similarly, but the increase in Tb was smaller
in those animals treated with CTAP. The results indicate that mu, but not kappa, opioid receptors
in the PAG seem to act as pyrogenic agents during fever induced by restraint stress in rats.
03.013 - INTERLEUKIN-1 RECEPTOR ANTAGONIST DECREASES HYPOTHALAMUS OXIDATIVE
STRESS DURING EXPERIMENTAL SEPSIS
CARLOS HENRIQUE ROCHA CATALÃO; FAZAL WAHAB; RODRIGO PEREIRA DE ALMEIDA RODRIGUES;
NILTON NASCIMENTO SANTOS-JÚNIOR; LUIS HENRIQUE ANGENENDT DA COSTA; MARIA JOSÉ ALVES
ROCHA
Purpose: We investigated the effect of IL-1ra pre-treatment on the sepsis-induced increase in
oxidative stress markers in the hypothalamus of rats.
Methods: The animals were pre-treated by an icv injection of IL-1ra (9-nmol) or vehicle (0.01M
PBS) before being subjected to cecal ligation and puncture (CLP), or left as control (shamoperation or naive). After 4, 6 and 24h, the animals were decapitated (n=8/group) and the brain
removed for hypothalamic tissue collection. Transcript and protein levels of IL-1, inducible nitric
oxide synthase (iNOS), caspase-3 and hypoxia-inducible factor 1-alpha (HIF-1α) were quantified
by quantitative polymerase chain reaction (qPCR) and western blot, respectively.
Results: Hypothalamic mRNA levels of all these genes were significantly (p<0.005) increased at 4,
6 and 24h CLP, as compared to sham operated animals. IL-1ra pre-treatment in these CLP animals
significantly decreased IL-1 gene expression at all-time points and also of iNOS, caspase-3 and
HIF-1α at 24h when compared to vehicle treated CLP animals. The effect of the pre-treatment on
protein expression was most clearly seen for IL-1β and iNOS at 24h.
Conclusion: Our results showed that blocking the IL-1-IL-1r signaling pathway by central
administration of an IL-1ra decreases hypothalamic oxidative stress markers during sepsis.
03.014 - ROLE OF COMMISSURAL NUCLEUS OF SOLITARY TRACT ON MAINTENANCE OF
HYPERTENSION AND ON RESPIRATORY ACTIVITY IN 2K1C RATS
MARIANA DEL ROSSO MELO; SILVIA GASPARINI; GUILHERME F.F. SPERETTA; JOSÉ VANDERLEI
MENANI; DANIEL BRESEGHELLO ZOCCAL; DEBORA SIMÕES ALMEIDA COLOMBARI; EDUARDO
COLOMBARI
ADEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, UNESP, ARARAQUARA, SP, BRASIL.
We have demonstrated that 2K1C hypertensive rats present baseline higher ventilation and
increased ventilatory response to hypoxia, which suggests peripheral chemoreflex overactivity. In
the present we aimed to evaluate the role of comissural nucleus of solitary tract (cNTS) on the
maintenance of hypertension and on diaphragm activity in 2K1C rats. Male Holtzman rats (150180 g) received a silver clip around the left renal artery to induce renovascular hypertension (n=6).
Rats with sham surgery were used as normotensive control (NT, n = 7). Six weeks after the surgery,
saline (NaCl 0.15 M, 60 nl) followed 20 min later by isoguvacine (20 mM; 60 nl – gabaergic agonist)
were microinjected into the cNTS of urethane (1.4 g/Kg of body weight; i.v.) anesthetized rats
and mean arterial pressure (MAP) and diaphragm activity (DA) were recorded for an additional 60
min. Baseline MAP in 2K1C rats was higher than in NT rats (MAP: 193 ± 9, vs. NT: 123 ± 2 mmHg,
p<0.05). Twenty min after isoguvacine injection, there was a greater decrease in MAP in 2K1C
compared to NT rats (-40 ± 6, vs. NT: -16 ± 3 mmHg, p<0.05). DA also decreased after isoguvacine
injection in 2K1C animals (Δ -19 ± 6 vs. NT: 1 ± 7 %, 60 min; p<0.05). These data showed that cNTS
is an important source to the maintenance of renovascular hypertension and diaphragm activity
in 2K1C rats.
03.015 - NEURONS FROM PAG RECEIVE PROJECTIONS FROM SN AND PROJECT TO RTN
JULIANA CRISTINA DE LIMA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA; MARINA TARANTO
BOTELHO
Parkinson's disease (PD) is characterized by a reduction in the number of dopaminergic neurons of
the substantia nigra (SN) accompanied by motor and non-motor deficiencies such as respiratory
failure. Previous study showed that in a model of PD there is a decrease in respiratory rate and in
the number of retrotrapezoid nucleus (RTN) neurons. Preliminary study from our laboratory has
also shown that there are no direct projections from SN to RTN. Therefore, our objective was to
investigate if the pathway between SN and RTN involves a relay in the midbrain periaqueductal
gray (PAG), a region that plays an important role in physiological responses including breathing.
Adulto male Wistar rats (N=4/group, CEUA: 99, page22, book 03) received unilateral injection of
the anterograde tracer biotin-dextran-amide (BDA-10%) in the SN or PAG and retrograde tracer
6
Fluorogold (FG-2%) in the RTN and PAG. The results showed the presence of varicosities containing
BDA in the PAG (from BDA injection into the SN) and into the RTN (from BDA injection into the
PAG). The results from retrograde tracer showed that there are FG labeled neurons in the PAG
(from FG injection into the RTN) and FG+/TH+ labeled neurons in the SN (from FG injection into
the PAG).
In conclusion, our results showed that there is a direct projection from SN to PAG and from PAG
to RTN, which suggest that the PAG is a relay between SN and the chemoreceptors neurons into
the RTN.
03.016 - NEURÕNIOS KNDY SÃO ATIVADOS PELO ESTRADIOL DURANTE OS PICOS PRÉOVULATÓRIOS DE HORMÕNIO LUTEINIZANTE E PROLACTINA EM FÊMEAS
ROBERTA ARAUJO LOPES1; RAQUEL GIL DE LIMA BERNARDES1; FERNANDA LUIZA MENEZES BELLO1;
PATRÍCIA COSTA HENRIQUES1; NAYARA SOARES SENA AQUINO1; CELSO RODRIGUES FRANCI2;
RAPHAEL ESCORSIM SZAWKA1
1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE DE
SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL.
A kisspeptina (Kp) é essencial para secreção de hormônio luteinizante (LH) e fertilidade. Os
neurônios KNDy do núcleo arqueado (ARC) co-expressam kisspeptina, neurocinina e dinorfina, e o
seu papel no controle de secreção hormonal permanece obscuro. Utilizamos dupla marcação
imunohistoquímica para investigar a atividade dos neurônios KNDy associada a mudanças na
secreção de LH e prolactina (PRL) em ratos machos e fêmeas. Ratas ciclando foram perfundidas
nas fases de diestro (n=6), 12 e 18 h do proestro (n=5-6) e estro (n=6). O número de neurônios Kp
imunorreativos (Kp-ir) no ARC foi menor no proestro e no estro em relação ao diestro (P<0.01).
Por sua vez, a porcentagem de neurônios Kp-ir com co-expressão de c-Fos foi 45% maior às 18 h
do proestro (P< 0.05). Em machos (n= 6-8), o número de neurônios Kp-ir no ARC foi menor em
relação as fêmeas em diestro e a co-expressão de c-Fos foi baixa e inalterada durante o dia (P=
0.68). Ratas ovariectomizadas (OVX) foram tratadas com óleo (OVX; n=8), estradiol (OVX+E; n=7)
ou estradiol e progesterona (OVX+EP; n=6-7) e foram perfundidas às 12 e 18 h. O número de
neurônios Kp-ir no ARC foi menor nos grupos OVX+E e OVX+EP em relação as ratas OVX (P<0.05).Por
outro lado, a expressão de c- Fos nos neurônios KNDy aumentou em aproximadamente 50% às 12
e 18 h (P<0.05) em ratas OVX+E e somente às 12 h (P<0.01) em ratas OVX+EP.O estradiol reduz a
expressão de Kp no ARC, porém, a população remanescente de neurônios KNDy permanece ativa
no momento do pico de LH e PRL, o que demonstra um novo aspecto da regulação dos neurônios
KNDy pelo estradiol.
03.017 - CORRELATION BETWEEN NEUROANATOMICAL AND RESPIRATORY CHANGES IN AN
EXPERIMENTAL MODEL OF PARKINSON'S DISEASE
SILVIO DE ARAUJO FERNANDES-JUNIOR
UNIVERSIDADE DE SÃO PAULO, SAO PAULO, SP, BRASIL.
Parkinson's disease (PD) is a disorder characterized by motor and non-motor symptoms, such as
respiratory deficits and is associated with loss of dopaminergic neurons in the substantia nigra
(SN). Previous study showed that 40 days after the induction of an experimental model of PD in
rats there is a decrease in the resting respiratory rate that lasted at least for 60 days. After 60
days, the immunohistochemical analysis showed a decrease in the number of Phox2b+-neurons of
retrotrapezoid nucleus (RTN) and nucleus of the solitary tract (NTS). The aim of this study is to
investigate if the neuroanatomical deficiency occurs before the functional respiratory abnormality
observed. Adult male Wistar rats (n=13, CEUA 104/23/03) with bilateral injections of 6-hydroxydopamine (6-OHDA, 24 µg/µl) into the striatum were used. Respiratory parameters were evaluated
one day before, 30 and 40 days after the injections. 6-OHDA produced 70% of reduction in
catecholaminergic neurons of SN and reduced resting respiratory rate at 40 days after the lesion
(79 ± 3 and 111 ± 19 vs. vehicle: 106 ± 5). Neuroanatomical results showed that 30 and 40 days
after the lesion, the animals not presented a reduction of Phox2b+-neurons in the RTN (29±6 and
31±2, respectively vs. vehicle: 60±1) but yes in the NTS (387± 12 and 360±2, respectively vs.
vehicle: 436±16). Our data suggest that neuroanatomical changes in NTS can be a cause for
respiratory problems observed in PD. Key-words: Parkinson's disease, retrotrapezoid nucleus,
respiratory changes.
03.018 - NEONATAL OVERNUTRITION IMPAIRS HYPOTHALAMIC LEPTIN RESPONSIVENESS IN
ADULTHOOD
PAULA BEATRIZ MARANGON; BEATRIZ C. BORGES; JOSÉ ANTUNES-RODRIGUES; LUCILA LEICO
KAGOHARA ELIAS
FMRP - USP, RIBEIRAO PRETO, SP, BRASIL.
Neonatal nutrition modifies circulating leptin levels, leading to changes in the maturation of
hypothalamic circuitry and metabolic disturbances in adulthood. Using the manipulation of litter
size as a model of nutritional programing, we aimed to investigate in male Wistar rats plasma
leptin levels and the hypothalamic responsiveness to leptin. After birth, litters were adjusted as
follows: small (3 pups-SL), normal (10 pups-NL) and large (16 pups-LL). Blood samples were
collected at 8am on postnatal day 7 (P7), P10, P14, P21, P60 for Elisa assay. The following
experiments were performed on P60. Animals were treated with leptin (500µg/Kg ip) or saline at
5pm. Food intake and body weight gain were evaluated 12h later; the BAT was collected for
Western blotting 24h later. In another set of rats, 40min after leptin, animals were perfused and
the brains collected for immunohistochemistry. A peak in plasma leptin on P10 was only observed
in NL rats. SL rats had increased leptinemia in all days analyzed. After leptin, NL and LL rats
reduced food intake and body weight gain, with no effect in SL rats. SL and LL rats had increased
p-STAT-3 expression in the ARC in vehicle treated animals. Leptin increased p-STAT-3 expression
in the ARC in NL rats and potentiated it in LL rats, with no effect in SL rats. Leptin increased UCP1 expression in the BAT in NL and LL rats. Our data indicate that neonatal changes in food
availability modify hypothalamic leptin responsiveness in obese animals in adulthood.
03.019 - EFFECTS OF HIGH SALT DIET DURING THE POSTNATAL PERIOD ON WATER AND SODIUM
INTAKE INDUCED CELLULAR AND EXTRACELLULAR DEHYDRATION IN THE ADULT PHASE
ARYANNE BATISTA SOARES DE MELO1; THAIS MACHADO MERCÊS1; GRAZIELA TORRES BLANCH2;
GUSTAVO RODRIGUES PEDRINO1; ANDRE HENRIQUE FREIRIA-OLIVEIRA1
1.UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL; 2.PONTIFÍCIA UNIVERSIDADE CATÓLICA
DE GOIÁS, GOIANIA, GO, BRASIL.
Studies suggest that lived experiences in the initial stages of life, including the prenatal stage,
are related to behavioral and pathophysiological changes in adulthood. This study investigated
drinking and salt appetite induced by cellular and extracellular dehydration in rats submitted to
high salt diet on postnatal age. Wistar male rats (21 days old) were kept with hypertonic saline
solution (0.3 M NaCl, EXP) and food for 60 days. Control group was kept with water and food.
Water and food was given for 15 more days (recovery). Subsequently, the animals received
furosemide (10 mg, subcutaneously) and were kept in metabolic cages with access to water and
poor sodium diet for 24 hours. After 24 hours, graduated burets with water and hypertonic saline
(NaCl 0.3 M) were offered for 2 hours. EXP rats drank less water (EXP: 0.8 ± 0.5 vs. control: 1.5 ±
0.6 ml) and hypertonic saline (EXP: 4.5 ± 1.3 vs. control: 7.5 ± 2.6 ml) than control animals induced
by extracellular dehydration. The cellular dehydration was induced by 2 M NaCl load (2 ml) gavage.
The animals are maintained in metabolic cages, and one hour after the gavage, water was offered
to animals for 2 hours. We did not observe any difference on water intake (EXP: 10.5 ± 1.6 vs.
control: 9.9 ± 1.5 ml). The results suggest that the extracellular thirst and sodium appetite are
affected by postnatal dehydration experiences.
03.020 - NEURON-GLIA INTERACTION IN NUCLEUS TRACTUS SOLITARIUS IS REDUCED BY SHORTTERM SUSTAINED HYPOXIA
DANIELA ACCORSI-MENDONÇA; CARLOS EL ALMADO; LENI GH BONAGAMBA; JACI AIRTON CASTANIA;
DAVI JA MORAES; BENEDITO HONORIO MACHADO
UNIVERSITY OF SAO PAULO, ESTRANGEIRO, SP, BRASIL.
Sustained hypoxia (SH) produces significant changes in physiological functions due to peripheral
chemoreflex activation. We analyzed the effect of short-term SH (24 hours, FIO210%) on the
processing of cardiovascular and respiratory reflexes using an in situ preparation of rats. SH
increased both the sympatho-inhibitory and bradycardiac components of baroreflex as well as
sympathetic and respiratory responses to chemoreflex activation. We also analyzed the
neurotransmission in nucleus tractus solitarius (NTS) neurons using brainstem slices and wholecell patch clamp. The 2nd-order NTS neurons were identified by previous application of tracer
onto carotid body for or aortic depressor nerve. SH increased the frequency of action potential
(AP) after positive injected current in NTS neurons. Delayed excitation, related to A-type
potassium current (IKA) and sensitive to 4-aminopyridine, was observed in most of NTS neurons
from control rats and IKA amplitude was higher in identified 2nd-order NTS neurons in control
than in SH rats. SH also blunted the astrocytic inhibition of IKA in NTS neurons and increased the
synaptic transmission in response to afferent fibers stimulation. The frequency of spontaneous
excitatory currents was also increased in neurons from SH rats with no change in amplitude or
half-width. Therefore, SH changed the glia-neuron interaction, increasing the intrinsic excitability
and excitatory transmission of NTS neurons, which may contribute to the observed increase in the
reflex sensitivity of baro- and chemoreflex in in situ preparation.
THEME 04 – CARDIOVASCULAR PHYSIOLOGY
04.001 - A INTENSIDADE DO EXERCÍCIO PROMOVE DIFERENTES ALTERAÇÔES NOS PARÂMETROS
DE CONTRATILIDADE MIOCÁRDICA EM RATAS TREINADAS POR NATAÇÃO
PAULA ANDRÉA MALVEIRA CAVALCANTE1; MAURO SÉRGIO PERILHÃO1; LEONARDO DOS SANTOS2;
ANDREY JORGE SERRA3; PAULO JOSÉ FERREIRA TUCCI4; DANILO SALES BOCALINI1
1.UNIVERSIDADE SÃO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DO
ESPÍRITO SANTO, VITÓRIA, ES, BRASIL; 3.UNIVERSIDADE NOVE DE JULHO, SÃO PAULO, SP, BRASIL;
4.UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO PAULO, SP, BRASIL.
As adaptações miocárdicas relativas às diferentes intensidades de exercício ainda são
controversas. Assim, o objetivo do estudo foi avaliar as adaptações da contratilidade miocárdica
de ratas treinadas por natação com diferentes intensidades de exercício. Ratas Wistar foram
distribuídas aleatoriamente em três grupos experimentais: Controle(C) e treinados (8sem,5
dias/sem,60 min/dia) moderadamente (Tm:sem peso adicional à cauda) e intensamente (Ti:com
carga relativa a 8% do peso corpóreo adicionado). Foram determinadas a capacidade física
(CF:seg), as tensões (g/mm2/mg) desenvolvida (TD) e repouso(TR), as derivadas (g/mm2/mg/s)
positivas (+dT/dt) e negativas (-dT/dt). Após oito semanas de treinamento a CF
(C:141±12;Tm:293±10;Ti:371±20;seg) diferiu entre os grupos . Os valores da TD(0,85±0,4), +dT/dt
e -dT/dt(7±0,97 e 5±0,34) do grupo C foram inferiores aos grupos Tm(1,07±0,1;11±1,07 e -7±0,52)
e Ti(1,27±0,7;15±0,59 e -10±1,06). A atividade do retículo sarcoplasmático do grupo Ti foi
aprimorada em 52±3% diferindo dos grupos Tm(43±4%) e C(32±4%) que também diferiram entre si.
Em relação às curvas de estiramento tensão, não foram encontradas diferenças entre os grupos,
nos coeficientes angulares da TD e da rigidez miocárdica. Em conclusão, a variação da intensidade
do programa de treinamento exerce influência tanto na capacidade física quanto no desempenho
contrátil e pode ser atribuída à melhora dos mecanismos envolvidos na cinética do Ca2+
04.002 - EFEITOS DE EXPOSIÇÃO DA FUMAÇA DE CIGARRO DE CURTO PRAZO NA FUNÇÃO RENAL
E MIOCÁRDICA EM RATOS
ARIANA ALINE SILVA1; LILIAN LUIZ DA SILVA ALVES1; MAURO SÉRGIO PERILHÃO1; RAFAEL LUIZ
SILVA2; EDNEI LUIZ ANTONIO2; JAIRO MONTEMOR DA SILVA2; ANDREY JORGE SERRA3; LEONARDO
DOS SANTOS4; NESTOR SCHOR2; PAULO JOSÉ FERREIRA TUCCI2; DANILO SALES BOCALINI1
1.UNIVERSIDADE SÃO JUDAS TADEU, MOÓCA, SP, BRASIL; 2.UNIFESP, SÃO PAULO, SP, BRASIL;
3.UNINOVE, SÃO PAULO, SP, BRASIL; 4.UFES, VITÓRIA, ES, BRASIL.
Estudos recentes apresentaram efeitos diretos do tabagismo sobre o sistema cardiovascular. No
entanto, as alterações miocárdicas e renais permanecem ainda inconclusivas. O objetivo deste
estudo foi investigar os efeitos da exposição da fumaça do cigarro a curto prazo sobre a função
miocárdica e renal de ratos. Para tanto, ratas Wistar foram randomizados em 2 grupos, controle
(C) e 1 grupo experimental que foi exposto a fumaça de cigarro durante 8 semanas (CS). Após o
período de exposição de 8 semanas (2 períodos totalizando 40 cigarros diários, 5 dias por semana),
análises bioquímicas, funcionais e morfológicas foram realizadas através da ecocardiografia,
contratilidade miocárdica e analise da urina. A massa do ventrículo esquerdo (2,22 ± 0,11 mg/g)
e cardíaca (2,88 ± 0,15 mg/g) do grupo CS foi significativamente maior (P <0,05) em comparação
ao grupo C (1,89 ± 0,12 mg/g; 2,52 ± 0,19 mg/g). Foram encontradas reduções significativas do
diâmetro do ventrículo esquerdo do grupo CS na diástole (0.62 ± 0.03 mm) e na sístole (0.27 ±
0.03 mm) em relação ao grupo C (0.77 ± 0.09 mm; 0.40 ± 0.04 mm) respectivamente. Não foram
encontradas diferenças nos parâmetros hemodinâmicos e da função do miocárdio e renal. Em
conclusão, 8 semanas de exposição da fumaça do cigarro induzremodelação do miocárdio sem
disfunção ventricular esquerda e anormalidades no desempenho contrátil do miocárdio,
adicionalmente, o protocolo de exposição também não foi capaz de induzir alterações na função
renal.
04.003 - PREDICTORS AND REFERENCE VALUES OF PULSE WAVE VELOCITY IN PREPUBERTAL
CHILDREN ANGOLAN
AMÍLCAR BERNARDO TOMÉ DA SILVA1; DANIEL PIRES CAPINGANA2; PEDRO MAGALHÃES3; JOSÉ
GERALDO MILL4
1.PPGCF-UFES, VITORIA, ES, BRASIL; 2.FAC. MEDICINA/UAN, LUANDA, ANGOLA; 3.FAC,
MEDICINA/UAN, LUANDA, ANGOLA; 4.PPGCF/UFES, VITÓRIA, ES, BRASIL.
Background: Carotid-femoral pulse wave velocity (cf-PWV) is the gold standard method used in
clinical practice and epidemiological studies to determine arterial stiffness. However the lack of
reference values for specific groups, difficult to identify the cutoffs for cardiovascular risk. The
goal is to establish reference values for cf-PWV and determine its main predictors in prepubertal
children. Methods: A cross-sectional, observational and descriptive study in a sample of black
children enrolled in a public school of the 1st cycle of primary education in the urban area of
Luanda, the Angolan capital. We evaluated 157 children 7-11 years of age (9.30 ± 1.1 years), Stage
I of Tanner. To measure the cf-PWV was used an automatic device, brand Complior SP. Results:
Mean cf-PWV was 4:59 ± 0:54 m/s with no difference between gender. Graphics with values of
percentiles for height and age were presented. The univariate regression analysis model
correlated positively cf-PWV (P <0.05) with age, weight, lean body mass, height, systolic blood
pressure, diastolic and mean. Conclusion: After the multivariate analysis, only the height
maintained as main predictors of PWV in prepubertal children. This is one of the few studies
proposing reference values of PWV in this age group.
04.004 - A EXPOSIÇÃO PRÉVIA A FUMAÇA DE CIGARRO INTENSIFICA O COMPROMETIMENTO DA
CAPACIDADE FÍSICA E DA FUNÇÃO VENTRICULAR DE RATOS INFARTADOS
MAURO SÉRGIO PERILHÃO1; PAULA ANDRÉA MALVEIRA CAVALCANTE1; PAULO JOSÉ FERREIRA
TUCCI2; JAIRO MONTEMOR DA SILVA2; ANDREY JORGE SERRA3; LEONARDO DOS SANTOS4; EDNEI LUIZ
ANTONIO5; DANILO SALES BOCALINI1
1.UNIVERSIDADE SÃO JUDAS TADEU, SÃO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DE SAO
PAULO, SÃO PAULO, SP, BRASIL; 3.UNIVERSIDADE NOVE DE JULHO, SÃO PAULO, SP, BRASIL;
4.UNIVERSIDADE FEDERAL DO ESPIRITO SANTO, VITORIA, ES, BRASIL; 5.UNIVERSIDADE FEDERAL DE
SÃO PAULO, SÃO PAULO, SP, BRASIL.
As repercussões da exposição à fumaça realizada previamente ao infarto ainda permanecem
inconclusivas. O objetivo do estudo foi investigar os efeitos da exposição previa da fumaça de
cigarro previamente ao infarto na capacidade física, na dilatação e na função ventricular. Oitenta
e três ratas foram randomizadas em quatro grupos: controle (C:n=10), fumantes (F:n=18, expostos
à fumaça), infarto (IM,n=26, submetidos cirurgia de oclusão coronária) e fumante infarto
(FIM,n=29, expostos à fumaça e oclusão coronária). O protocolo de exposição de 16 semanas
(40cigarros,2xdia,5xsem). Prejuízos foram encontrados no grupo F (199+18 vs 141+12). Após a
interrupção da exposição o desempenho do grupo F foi recuperado, contudo foi menor que o grupo
C (202+15) e semelhante aos grupos IMm (158+4) e FIMm (161+4) que não diferiram entre si. Ao
analisarmos os grupos IMg diferenças estatísticas (p<,001) foram encontradas entre os demais
grupos, entretanto, o grupo FIMg (110+5) apresentou desempenho inferior em relação ao grupo
IMg (134+3). O período de exposição à fumaça de cigarro induziu o desenvolvimento de hipertrofia
concêntrica com redução na FEAT. Após a interrupção da exposição os valores da FEAT dos grupos
C e F foram maiores que os grupos IMm (32+6) e FIMm (36+7) que não diferiram entre si, mas
7
foram superiores aos grupos IMg (21+4) e FIMg (17+4). Em conclusão animais submetidos à fumaça
previamente ao infarto apresentam comprometimento na CF e na função ventricular.
04.005 - ALTERAÇÔES DOS PARÂMETROS HEMATOLÓGICOS DE RATOS WISTAR: INFLUENCIA DO
ENVELHECIMENTO
MAURO SÉRGIO PERILHÃO1; LILIAN LUIZ DA SILVA ALVES 1; ARIANA ALINE SILVA1; WILSON JACOB
FILHO2; ROMEU SOUZA1; DANILO SALES BOCALINI1
1.UNIVERSIDADE SÃO JUDAS TADEU, SÃO PAULO, SP, BRASIL; 2.UNIVERSIDADE DE SÃO PAULO, SÃO
PAULO, SP, BRASIL.
Pesquisas com modelo animal têm sido amplamente utilizadas na avaliação dos efeitos do
crescimento e envelhecimento de tecidos e órgãos, favorecendo o avanço do conhecimento dos
mecanismos vitais, aperfeiçoamento dos métodos de prevenção, diagnóstico e tratamento de
doenças. Objetivo: avaliar as alterações dos parâmetros hematológicos de ratos machos Wistar
de acordo com o processo de envelhecimento.Material e métodos: Para tanto foram estudados
140 ratos Wistar Machos, distribuídos em seis grupos: 2, 3, 6, 12, 18, e 24 meses de vida. Todos
os animais foram previamente sedados e extraído amostras de sangue da extremidade distais da
cauda, permitindo o livre fluxo de sangue, sendo a posteriori, preparados esfregaços para a
contagem diferencial dos leucócitos e o percentual de reticulócitos. Resultados: no diferencial de
Concentração de Hemoglobina Corpuscular Média (2: 32,18±2,44 ; 3:3,31±2,5; 6;31,48±1,17
;12:31,18±1,02
;18:
30,08±1,31;
24:35,46±1,53),
Eosinofícilos(2:
0,6±
0,69;
3:0,93±1,03; 6:2,88±2,31 ;12: 2,16±1,160,91; 18: ,8±2,28; 24; 4,2±2,86), linfócitos (2: 10,12±2,21
; 3: 11,45±3,69 ; 6: 11,66±5,27;12: 11,98±5,87 ;18: 7,76±0,96; 24:10,56±7,05) e monócitos (2:
2,9±1,28; 3:3,06±2,31; 6:3,22±0,83;12: 2,33±1,36;18: 2,8±2,04;24: 2,4±1,14).Conclusão: Foram
identificadas discretas alterações nos parâmetros hematopatologicos de acordo com o processo
de envelhecimento, contudo, os dados do presente estudo aponta para o estabelecimento de
valores hematológicos de referência para estudos com este tipo de modelo animal.
04.006 - FETAL HEART PROGRAMMING BY PROTEIN RESTRICTION DIET: EFFECTS ON HEART
STRUCTURE AND BLOOD PRESSURE IN MALE YOUNG ADULT OFFSPRING
KAMILA FERNANDA ROSSINI; HÉRCULES JONAS REBELATO; MARCELO AUGUSTO MARRETO
ESQUISATTO; ROSANA CATISTI
FUNDAÇÃO HERMÍNIO OMETTO, ARARAS, SP, BRASIL.
The dietary limitation during pregnancy influences the growth and development of the fetus and
offspring and their health into adult life. The adverse effect of fetal protein restriction exposures
and mechanisms underlying these associations on heart development and functional disorder are
unclear. The present study investigates in young adult male rats, the effect of gestational protein
restriction (GPR) on blood pressure (BP) by an indirect tail-cuff method using an electro
sphygmomanometer and its association with heart structure histological techniques. Dams (n=12)
were kept on normal (NP), or low (LP) protein diet over all pregnancy phase. BP of pups was
measured from 4th to 8th week. The current study showed in at 8-week old LP offspring showed
no difference in body weight, though newly born was lighter. Arterial BP enhanced significantly
in LP (131.8±2.7 mmHg) than in NP (120.3±3.33 mmHg) animals. The myocardium did not present
macroscopical alterations between groups on: heart/body weight (NP, 3.89 0.48 mg/g; LP,
3.86±0.28 mg/g) and left ventricle/body weight (NP, 2.28±0.25 mg/g; LP, 2.49±0.27 mg/g). It was
observed a significant decrease in the number of myocytes by 10 4µm2. The area occupied by
collagen fibers increased significantly. These results suggest that GPR affects myocardium
organization in male youngs adult offspring heart. Approved by CEUA/UNIARARAS (056/2014) and
supported by FHO|Uniararas.
04.007 - VENTRICULAR REMODELING OF RATS FED AT LOW PROTEIN DIET IN LATE PREGNANCY
ANA CLAUDIA APARECIDA TEIXEIRA; MARCELO AUGUSTO MARRETO ESQUISATTO; HÉRCULES JONAS
REBELATO; ROSANA CATISTI
CENTRO UNIVERSITÁRIO HERMÍNIO OMETTO, ARARAS, SP, BRASIL.
The heart size is increased during pregnancy, but it is unclear if such process would be due to
heart muscle hypertrophy or changes that result from the anatomic alterations which normally
occurs in this period. This study investigated whether gestational protein restriction alters left
ventricle hypertrophy at end of gestation. Pregnant dams were divided into two groups: normal
(NP, 17% casein) or low-protein diet (LP, 6% casein). At day 21 of gestation (21 dG), after
anestesia, the animals were perfused and the hearts collected, weighed and processed by
Toluidine blue (myocytes quantification) and Picrossirius-hematoxylin (collagen fibers
quantification). Cardiomyocytes cross-sectional area was determined by hematoxylin-eosin. The
myocardium presented structural alterations in the LP group compared to NP. The results showed
on 21dG a significant decrease in the number of myocytes by 104µm2. The area occupied by
collagen fibers increased significantly at the end of gestation in LP dams. These results show that
protein restriction affects myocardium organization in maternal heart and suggest degenerative
process, compatible with fibrosis. Study approved by CEUA/UNIARARAS (062/2013).
04.008 - MECANISMOS SINÉRGICOS LEVANDO A UM MAIOR RISCO DE AVC EM MULHERES IDOSAS
JULIANA STEFANIN FUZATTI; SANDRA GOMES DOS SANTOS; LUCIANA APARECIDA CAMPOS; VALTER
LUIS PEREIRA JR.; OVIDIU CONSTANTIN BALTATU
CAMILO CASTELO BRANCO UNIVERSITY (UNICASTELO), SAO JOSE DOS CAMPOS, SP, BRASIL.
Doença carótida arteriosclerótica tem sido associada a um risco aumentado de acidente vascular
cerebral. O objetivo deste estudo foi investigar a relação entre a doença carotídea e marcadores
cardíacos e vasculares de risco não-ateroscleróticos em mulheres idosas.
Doença carotídea foi determinada medindo-se o espessamento da carótida íntima-média (EIM). O
EIM das carótidas esquerda e direita, o escore de placa e índice de resistência (IR da carótida
comum, externa e interna), espessura da parede do ventrículo esquerdo (VE) e diâmetro da aorta
ascendente foram determinados pela ultra-sonografia duplex e modo-B.
A idade das mulheres e dos homens foi de 71 ± 11 e 68 ± 11, respectivamente. O EIM da carótida
direita foi correlacionada com o escore de placa em ambos os sexos (r de Spearman = 0,36 em
mulheres e 0,37 em homens, p = 0,01). O EIM da carótida direita teve uma correlação
significantemente positiva com a espessura da parede do VE e do diâmetro da aorta ascendente
só em mulheres (de Spearman r = 0,31 e 0,31, p <0,05), mas não em homens. O EIM da carótida
direita, espessura do VE e diâmetro da aorta foram positivamente correlacionados com a idade
apenas em mulheres (r de Spearman = 0,33, 0,40 e 0,26, respectivamente, p <0,05).
Estes dados indicam que marcadores não-ateroscleróticos de risco como hipertrofia cardíaca,
dilatação da aorta e idade estão associados com maior EIM em mulheres, mas não em homens. A
ação sinérgica desses fatores pode contribuir para uma piora na deterioração da carótida em
mulheres idosas.
04.009 - NORADRENALINE INJECTED IN THE RETROTRAPEZOID NUCLEUS INHIBITS INSPIRATION
LUIZ MARCELO OLIVEIRA; THIAGO S MOREIRA; ANA CAROLINA T TAKAKURA
Retrotrapezoid nucleus (RTN) is a region located in the ventrolateral medulla and is known to
contribute to central chemoreception, inspiration and active expiration. Previous studies showed
the presence of catecholaminergic varicosities in the RTN region and also showed that
noradrenaline (NA) can increase respiratory rate by the activation of α1 adrenergic receptors in
the pre-Bötzinger complex. The aim of this study was to investigate the cardiorespiratory effects
produced by the injection of NA in RTN. Mean arterial pressure (MAP), diaphragm (DiaEMG) and
abdominal (AbdEMG) muscle activities were recorded in urethane-anaesthetized, vagotomized
and artificial ventilated male Wistar rats (n = 6-8, CEUA: 14, p.15, b.03/2014). Unilateral injection
of NA produced a transient dose-dependent (50-1000 µM) inhibition of DiaEMG (26.4 ± 7.9%; 40.6
± 8.1% and 65.0 ± 2.0%, respectively), but did not change MAP and AbdEMG. One minute after the
highest dose (1000 µM) of NA into the RTN we observed a generation of sighs (larger amplitude in
DiaEMG) (0.64 ± 0.07; vs. control: 0.33 ± 0.05 mV) that lasted for 286 ± 35 seconds. We conclude
that the activation of adrenergic receptors in the RTN region inhibits inspiratory activity; and the
highest dose of NA tested generated sighs during normal respiration activity.
04.010 - SELECTIVE DENERVATION OF THE AORTIC AND CAROTID BARORECEPTORS
JACI AIRTON CASTANIA1; PEDRO LOURENÇO KATAYAMA1; FERNANDA BROGNARA1; JOÃO PAULO
JACOB SABINO2; RUBENS FAZAN1; HELIO CESAR SALGADO1
1.FMRP/USP, RIBEIRAO PRETO, SP, BRASIL; 2.FORP/USP, RIBEIRAO PRETO, SP, BRASIL.
The usual surgery for sinoaortic denervation (SAD) in rats leads to concomitant denervation of the
carotid chemoreceptors. This study reports a new technique to denervate the carotid and aortic
baroreceptors selectively maintaining the carotid chemoreceptors intact. Wistar rats were
subjected to selective aortic and carotid baroreceptor denervation maintaining the carotid
chemoreceptors intact (BAROS-X), or sham surgery (SHAM). Rats were implanted with femoral
artery and vein catheters for arterial pressure recording and drug administration. Baroreflex or
carotid chemoreflex activation was elicited by injection of phenylephrine or potassium cyanide
(KCN). Phenylephrine caused hypertension in SHAM (Δ = 46 ± 3 mmHg) and BAROS-X (Δ = 38 ± 3
mmHg) rats combined with reflex bradycardia in SHAM rats (Δ = -54 ± 11 bpm) but no change in
heart rate in BAROS-X (Δ = -5 ± 2 bpm) rats. The hypertensive (39 ± 5 mmHg in BAROS-X vs 44 ± 6
mmHg in SHAM rats) and bradycardic (-103 ± 23 bpm in BAROS-X vs -88 ± 17 bpm in SHAM rats)
responses to KCN were similar in both groups confirming that the carotid chemoreceptors were
intact. These results provide support to a new surgical technique in rats, to inactivate the aortic
and carotid baroreceptors, keeping intact the carotid chemoreceptors.
04.011 - STRUCTURAL ORGANIZATION OF THE HEART IN LATE PREGNANCY OF RATS FED 50%
CALORIC RESTRICTION
LARISSA RUELLA DE ASSIS; MARCELO AUGUSTO MARRETO ESQUISATTO; HÉRCULES JONAS
REBELATO; DANIELLA NUNES ROLLO NEODINI; ROSANA CATISTI
Pregnancy is characterized by left ventricular hypertrophy that is potentially accounted for by
cardiomyocyte hypertrophy. The increase of maternal total blood volume and cardiac output
during gestation may result from two mechanisms acting in concert: 1) the production of several
hormones by the fetus and the placenta, and 2) the uteroplacental circulation acting as an
arteriovenous shunt. This study investigated whether gestational caloric restriction alters left
ventricle hypertrophy and collagen fibers distribution at end of gestation. Pregnant female Wistar
rats were divided in control group (C, n = 6, basal diet) and restricted group fed 50% of the diet
consumed by control (R, n = 6). At day 21 of gestation (21 dG), after anestesia, the animals were
perfused and the hearts collected, weighed and processed by Toluidine blue (myocytes
quantification) and Picrossirius-hematoxylin (collagen fibers quantification). Cardiomyocytes
cross-sectional area was determined by hematoxylin-eosin and the results showed on 21dG a
significant decrease in R group. The number of myocytes by 104µm2 were decreased in these
animals. The area occupied by collagen fibers increased significantly at the end of gestation in
restricted mothers. These results show that caloric restriction affects myocardium organization
in maternal heart, clear evidence of a degenerative process. Study approved by CEUA/UNIARARAS
(055/2014).
04.012 - T3 AND T4 EFFECTS IN INFLAMMATORY AND PRO-APOPTOTIC PROTEINS EXPRESSION
AFTER MYOCARDIAL INFARCTION
VANESSA DUARTE ORTIZ; ALEXANDRE LUZ DE CASTRO; RAFAEL DE OLIVEIRA FERNANDES; CRISTINA
CAMPOS CARRARO; RAFAELA SIQUEIRA; JÉSSICA H.POLETTO BONETTO; ADRIANA CONZATTI; TÂNIA
REGINA GATELLI FERNANDES; ADRIANE BELLÓ-KLEIN; ALEX SANDER DA ROSA ARAUJO
UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL.
Introduction: Several studies have demonstrated cardioprotective effects of thyroid hormones
(TH) after myocardial infarction. Objective: The aim of this study was to evaluate TH effects on
cardiac function after myocardial infarction, as well as evaluate inflammatory and pro-apoptotic
proteins expression. Methods: Male Wistar rats (300-350g) (n=8/group) were divided into four
groups: Sham-operated (SHAM), infarcted (AMI), sham-operated + TH (SHAMT) and infarcted + TH
(AMIT). During 12 days, the animals received T3 and T4 (2 and 8 µg/100g/day) by gavage. After
this, the rats were submitted to hemodynamic and echocardiographic analysis, were sacrificed
and the left ventricle (LV) was collected to molecular analysis. Statistical analyses: two-way
ANOVA with Student-Newman-Keuls post test. Ethics Committee number: 23262. Results: Ejection
Fraction was increased in AMIT rats compared with AMI. LV systolic pressure was also increased
and the end-diastolic pressure was reduced in AMIT when compared with AMI. The treated rats
presented a decrease in toll like receptor 4 and in nuclear transcription factor kappa beta
expression when compared with no treated groups. Besides that, AMI rats presented an increase
in the pro-apoptotic proteins, p53 and JNK. The hormonal treatment prevented this increase.
Conclusion: TH administration improved cardiac functional parameters, as well as decreased the
expression of inflammatory and pro-apoptotic proteins after myocardial infarction.
04.013 - THYROID HORMONES EFFECTS ON PERIPHERAL VASCULATURE AFTER ACUTE
MYOCARDIAL INFARCTION
VANESSA DUARTE ORTIZ; ALEXANDRE LUZ DE CASTRO; RAFAELA SIQUEIRA; JÉSSICA H.POLETTO
BONETTO; ADRIANA CONZATTI; TÂNIA REGINA GATELLI FERNANDES; ADRIANE BELLÓ-KLEIN; ALEX
SANDER DA ROSA ARAUJO
UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL.
Introduction: The treatment with thyroid hormones (TH) have presented positives effects over the
myocardium after acute myocardial infarction (AMI), but there is no data regarding the influence
of AMI and this treatment over the peripheral vasculature.Objective: The main goal was to analyze
the influence of these factors on the peripheral vasculature through parameters of oxidative
stress, angiogenesis and TH receptors (TRα,TRβ) and endothelial nitric oxide synthase (eNOS)
expression.Methods: Male Wistar rats (~350g)(n=11-16/group) were divided into four groups:
Sham-operated (SHAM), infarcted (AMI), sham-operated+TH (SHAMT) and infarcted+TH (AMIT).
During 12 days, the animals received T3 and T4 (2 and 8µg/100g/day) by gavage. After, the rats
were submitted to echocardiographic analysis. The aorta was collected to molecular analysis.
Statistical analyses: two-way ANOVA with Student-Newman-Keuls post test. Ethics Committee
number:23262.Results: The vascular endothelial growth factor, hypoxia-inducible factor 1α, TRα
and TRβ receptors expression increased in AMIT compared to AMI. The reactive oxygen species
production, NADPH oxidase activity and eNOS expression decreased in AMI compared with SHAMT,
while there is no deference between AMIT and AMI.Conclusion: The TH presented an action over
angiogenesis and TRα and TRβ expression on peripheral vasculature in a post-AMI period. These
may indicate an improvement in angiogenesis and a better responsiveness of peripheral
vasculature to HT.
04.014 - CHRONIC UNPREDICTABLE STRESS INCREASES CARDIAC SYMPATHETIC MODULATION IN
RATS WITH CHEMICALLY INDUCED OVARIAN FAILURE
BRUNA BARCELOS DE SIMAS 1; FLAVIANO LORENZON1; TAMIRES GREGORIO1; JANETE APARECIDA
ANSELMO-FRANCI2; FERNANDA BARBOSA LIMA CHRISTIAN1; DOMITILA AUGUSTA HUBER1
SAO PAULO, RIBEIRAO PRETO, SP, BRASIL.
Estrogen loss and stress leave women more susceptible to cardiovascular problems. Therefore, we
sought to determine whether the exposure to chronic unpredictable stress (CUS) during ovarian
failure induced by 4-vinylcyclohexene diepoxide (VCD) in rats could alter the autonomic control
of the cardiovascular system. Female rats (28 days old) were treated with VCD (160 mg/kg, ip) or
vehicle (CTL) for 15 consecutive days. After 70 days, the rats were exposed to daily sessions of
CUS for 10 days. After that, catheters were inserted into the femoral artery and vein. Recordings
of arterial pressure (AP) were performed 24 h later. Using the computer software CardioSeries
v2.4, we evaluated the high (HF) and low frequency (LF) bands of pulse interval (PI) and LF band
of systolic AP spectrum. Mean AP, HR and LF of systolic AP were comparable among groups. The
spectral analysis revealed no difference between VCD (9) and CTL (7) groups with no stress.
However, for the groups exposed to CUS, VCD (7) compared to CTL (6) showed an increase in LF
(47±3 vs 31±3 nu, P<0.05) associated with a decrease in HF of PI spectrum (53±3 vs 68±3 nu,
P<0.05), generating a higher LF/HF ratio (1.1±0.1 vs 0.6±0.1, P<0.05). Therefore, when exposed
to stress, rats with chemically induced ovarian failure presented an increased sympathetic
associated with a decreased parasympathetic modulation of cardiac function when compared to
normal rats, leading to higher risks for cardiovascular diseases.
8
04.015 - PAROXETINE IMPROVES CARDIAC CONTRACTILITY IN AORTIC REGURGITATION RATS BY
GRK2 INHIBITION
ANA CAROLINA MIEKO OMOTO1; LEONARDO NAZARIO MORAES 1; GEYSSON JAVIER FERNANDEZ1;
MELIZA GOI ROSCANI2; ROBSON FRANCISCO CARVALHO1; JULIANA IRANI FRATUCCI DE GOBBI1
1.IBB UNESP, BOTUCATU, SP, BRASIL; 2.UFSCAR, SÃO CARLOS, SP, BRASIL.
Aortic regurgitation (AR) culminates with eccentric hypertrophy due to a volume overload. Cardiac
hypertrophy is characterized by marked desensitization of β-adrenergic receptors (β-AR) and
impairment of cardiac contractility. G protein coupled receptor kinase 2 (GRK2) activities are
involved in dysfunctional adrenergic signaling. Paroxetine (parox), a consecrated selective
serotonin reuptake inhibitor, improves systolic function in AR rats. Recently, another action was
attributed to parox, it might act as a GRK2 inhibitor. Thus, we verify gene expression of GRK2 in
AR rats treated with parox. Male Wistar rats (230-250kg) were submitted either to AR surgery, by
retrograde puncture of the aortic valves leaflets, or sham surgery. The animals were divided in 4
groups: AR+parox, AR+saline, Sham+parox and Sham+saline. Parox (10mg/kg) was administered
subcutaneously for 4 weeks and saline was used as control. Morphofunctional variables of the
hearts were analyzed by echocardiograms at week 4 (before) and at week 8 (after) treatment. At
week 8 the animals were euthanized for tissue collection and posterior analysis of the gene
expression by RTq-PCR. Two way RM ANOVA was used for comparisons. The AR+parox group
presented an improvement in fractional shortening and decreased the GRK2 gene expression
(AR+parox: 1.14 ± 0.05 vs AR+saline: 1.39 ± 0.05). Our results show that parox treatment improves
systolic function of AR rats probably due inhibition of GRK2 activity.
04.016 - BAROREFLEX SENSITIVITY AND ADRENERGIC VASCULAR REACTIVITY IN RATS
SUBMITTED TO CROSS-SENSITIZATION BETWEEN PERIMENOPAUSE AND STRESS
TAMIRES GREGORIO1; FLAVIANO LORENZON1; BRUNA BARCELOS DE SIMAS 1; JANETE APARECIDA
ANSELMO-FRANCI2; DOMITILA AUGUSTA HUBER1; FERNANDA BARBOSA LIMA CHRISTIAN1
SAO PAULO, RIBEIRAO PRETO, SP, BRASIL.
During perimenopause, the chance of developing cardiovascular disease increases, especially with
the high incidence of stressors in this phase of life. Therefore, we studied cardiovascular
parameters in an experimental model of perimenopause exposed to chronic unpredictable stress
(CUS). Female rats (28 days old) were treated with 4-vinylcyclohexene diepoxide (VCD, 160
mg/kg, ip) or oil (CTL) for 15 consecutive days. After 70 days, the CUS was performed daily during
10 days and then catheters were inserted into femoral artery and vein. 24 h later, arterial pressure
(AP) and heart rate (HR) were recorded, and the baroreflex was evaluated by bolus injection of
phenylephrine (PE, 2 µg/kg) and sodium nitroprusside (SNP, 4 µg/kg). Hexamethonium (25 mg/kg)
was used to study the effect of ganglionic blockade on mean AP. Then, different doses of PE were
administered to access the adrenergic vascular reactivity -mediated pressor responses. The basal
AP and HR were comparable among the groups: VCD (n=10, 115±2 mmHg), CTL (n=7, 110±3 mmHg),
CUS-VCD (n=8, 117±3 mmHg), CUS-CTL (n=7, 110±3 mmHg). The tachycardia evoked by SNP and
bradycardia evoked by PE did not differ between VCD and CTL, exposed to CUS or not. In addition,
the contribution of sympathetic activity to basal AP and adrenergic vascular reactivity were
comparable among the groups. In conclusion, during perimenopause, the exposure to stress does
not produce changes in the studied parameters.
04.017 - H9C2 CELL LINE: HOW RELIABLE IS IT? PRELIMINARY STUDY WITH PAROXETINE
ANA CAROLINA MIEKO OMOTO; IVAN JOSE VECHETTI JR; LEONARDO NAZARIO MORAES; GEYSSON
JAVIER FERNANDEZ; PATRICIA FIDELIS DE OLIVEIRA GREGOLINI; ROBSON FRANCISCO CARVALHO;
JULIANA IRANI FRATUCCI DE GOBBI
IBB UNESP, BOTUCATU, SP, BRASIL.
The H9c2 is a clonal myoblastic cell line, which has been used to clarify molecular mechanisms
involved in pathophysiological hypertrophic growth. It has been showed that paroxetine (parox)
improves the contractility of the heart in cardiomyopathies. Therefore we treated H9c2 cells with
parox to study its influence on some gene related to the pathophysiology of hypertrophy. Thus
the gene expression of α-skeletal actin, ANP, BNP and the myosins isoforms (α-MyHC and β-MyHC)
were investigated in H9c2 cells. Growth media (DMEM) was used as vehicle, cells were seeded in
2 plates with 6 wells; at 80% of confluence they were exposed to the treatments. Phenilefrine
(phenil, 100μM), an inductor of hypertrophy, was added to the media for 24 hours and then parox
(300 μM) was added for another 24 hours. The study was divided into 4 groups: phenil+parox,
DMEM+parox, phenil+DMEM and DMEM+DMEM. At the end of the experiments cells were extracted
for molecular analyzes of gene expression, performed by RT-qPCR. There were no changes in the
expression of α-skeletal actin, a hypertrophy marker. The ANP, BNP and the myosin isoform genes
were not expressed in this cell line under our experimental conditions. Thus, H9c2 are
undifferentiated cells, derived from embryonic rat heart, which must be strongly challenged in
order to express characteristics of cardiomyocytes. Our present results arises some questions and
restrictions on using this cell line to understand specific cardiomyocyte genes.
04.018 - EFEITOS TARDIOS SOBRE RESPOSTAS HEMODINÂMICAS A AGENTES VASOATIVOS NA
PROLE EXPOSTA À FLUOXETINA DURANTE GESTAÇÃO E LACTAÇÃO
NATÁLIA KIMIE MATSUBARA1; LUIZ FERNANDO VERÍSSIMO1; VINICIUS LUCCA VOLPINI1; VIVIANE
BATISTA ESTRADA1; DENIS CARLOS DOS SANTOS1; ESTEFÂNIA GASTALDELLO MOREIRA1; DANIELA
CRISTINA CECCATTO GERARDIN1; MARCUS VINICIUS DE MATOS GOMES2; GRAZIELA SCALIANTI
CERAVOLO1; LEONARDO RESSTEL BARBOSA MORAES3; GISLAINE GARCIA PELOSI1
1.UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL; 2.UNIVERSIDADE NORTE DO
PARANÁ, LONDRINA, PR, BRASIL; 3.FACULDADE DE MEDICINA DE RIBEIRÃO PRETO/ UNIVERSIDADE
DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL.
A fluoxetina (FLX), um antidepressivo do tipo inibidor seletivo da recaptura de serotonina, é
utilizada para o tratamento da depressão, inclusive durante a gravidez. Ela penetra a barreira
hematoplacentária, expondo fetos e neonatos em desenvolvimento, mas os efeitos no sistema
cardiovascular da prole exposta à FLX ainda não são desconhecidos. Este estudo avaliou os efeitos
da exposição materna à FLX sobre respostas hemodinâmicas a agentes vasoativos na prole. Ratas
Wistar receberam por gavagem FLX (5mg/kg; FLX) ou água (CTR), da gestação até o desmame. No
dia pós-natal 60-75 inseriu-se na prole um cateter na artéria e veia femoral, para registro da
pressão arterial (PA) e infusão de fenilefrina (FE), nitroprussiato de sódio (NPS) e acetilcolina
(Ach). No dia seguinte, registrou-se a PA por 10min, seguido de infusão das drogas para análise
hemodinâmica. Para análise da curva dose-resposta usou-se teste ANOVA de duas vias e pós teste
Bonferroni. O Emáx e ED50 foram comparados com teste t de Student não pareado. Dados em
média ± EPM e P<0,05. Houve aumento da velocidade de resposta para FE e NPS, mas sem alterar
o ED50. Também houve mudanças no Emáx com Ach (FLX:-32,5±3,1, n=9; CTR:-42±3,23, n=9;
P=0,0492, T=2,128) e NPS (FLX:-56,33±3,407, n=14; CTR:-45,89±1,524, n=15; P=0,008, T=2,863).
Não houve mudanças na resposta hemodinâmica em fêmeas. Os resultados mostram que a
exposição à FLX altera respostas hemodinâmicas dependente de gênero.
04.019 - ESTUDO DAS ALTERAÇÔES CARDÍACAS ENCONTRADAS EM CAMUNDONGOS FÊMEAS QUE
SUPEREXPRESSAM VACHT
VANESSA PEREIRA TEIXEIRA; MARIANA GAVIOLI DE OLIVEIRA; MARCOS BARROUIN MELO; SILVIA
CAROLINA GUATIMOSIM FONSECA
O estrógeno é conhecido por promover efeitos cardioprotetores, sendo que um dos seus
mecanismos se dá pela modulação do sistema nervoso autônomo. Neste contexto, dados obtidos
pelo nosso grupo mostraram que camundongos com redução na atividade parassimpática, estão
protegidos dos efeitos deletérios da ovariectomia no coração. O objetivo deste trabalho foi
investigar se camundongos fêmeas que apresentam hiperatividade colinérgica, devido a
superexpressão do VAChT (SVT), apresentam alterações cardíacas e sua relação com estrógeno.
Para a realização desse estudo foram utilizados camundongos fêmeas selvagens (WT) e SVT com
3 meses de idade. Ambos os grupos apresentam ciclos estrais regulates. Análise ecocardiográfica
evidenciou que camundongos SVT apresentam aumento da fração de ejeção e de encurtamento
do ventrículo esquerdo, quando comparados aos WT. Foi realizada a coleta e pesagem do útero,
apresentando o grupo WT um maior peso com relação ao grupo SVT. Western blot para análise de
proteínas cardíacas mostrou que animais SVT apresentam aumento na expressão da SERCA e
redução da fosforilação da fosfolambam no resíduo Thr17, quando comparados aos WT. Corações
SVT também apresentam menor expressão da Monoamina oxidase (MAO) e maior expressão de
Tirosina Hidroxilase no resíduo Ser40 com relação ao grupo WT. Além disso, corações de
camundongos SVT apresentam alteração na expressão de receptores de estrógenos. Em conjunto
nossos dados sugerem que a superexpressão do VAChT leva a alteração nos parâmetros modulados
pelo estrógeno e maior contratilidade do músculo cardíaco.
04.020 - NANOCAPSULES WITH COPAIBA OIL PROMOTES CARDIOPROTECTION IN A MODEL OF
PULMONARY HYPERTENSION
CRISTINA CAMPOS CARRARO1; ANGELA MARIA VICENTE TAVARES 1; RAFAEL DE OLIVEIRA
FERNANDES1; RAFAELA SIQUEIRA1; ALEXANDRE LUZ DE CASTRO1; GIANA BLUME CORSSAC1; TÂNIA
REGINA GATELLI FERNANDES1; CLAUDIO PEREIRA2; ALEX SANDER DA ROSA ARAUJO1; ADRIANE
BELLÓ-KLEIN1
1.UFRGS, PORTO ALEGRE, RS, BRASIL; 2.TECNANO, PORTO ALEGRE, RS, BRASIL.
The aim of this study was to determine the antioxidant potential of copaiba oil and nanocapsules
containing this oil in a pulmonary arterial hypertension (PAH) model. Male Wistar rats (170g,
n=5/group) were divided in 6 groups: control ,monocrotaline (MCT), copaiba oil (CO), copaiba oil
+ monocrotaline (CO+MCT), nanocapsule with Copaiba (NCO) and nanocapsule + monocrotaline
(NCO+MCT). Animals received oil, nanocapsules or vehicle (by gavage) during 7 days. Then, PAH
was induced by MCT (60 mg/kg i.p.). Echocardiographic measurements were performed 21 days
after MCT injection. Afterwards, rats were killed to collect heart to evaluate morphometry and
to perform tiorredoxin (Trx), peroxirredoxin (Prx) and glutarredoxin (Grx) protein expression by
western blot.Both oil and copaiba nanocapsules significantly (P<0.05) reduced RV hypertrophy.
However, there was no significant difference between treatment with copaiba oil and
nanocapsules. Trx and Prx protein expression were significantly increased in MCT group, and it
was decreased in CO+MCT and NCO groups. Grx results showed no significant differences among
groups. Data suggest that both, copaiba oil and nanocapsules were able to reduce right ventricular
hypertrophy and increase antioxidant deffences in this model of PAH. This cardioprotective
mechanism needs to be further investigated.
04.021 - CARDIOVASCULAR CHANGES INDUCED BY PROTOTYPE ADMINISTRATION LQFM 163
STEFANNE MADALENA MARQUES; CASSIA MARIZ DA SILVA; NATHALIA ODA AMARAL; RICARDO
MENEGATTI; GUSTAVO RODRIGUES PEDRINO
UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL.
Objective: The LQFM 163 is a prototype drug similar to cilostasol. It has been demonstrated the
use of cilostazol for treatment of peripheral vascular disease. The present study evaluated
cardiovascular effects of LQFM 163 in normotensive rats. Methods: Wistar rats were anesthetized
with urethane (1.2 mg/kg; b.wt.) and instrumented to mean arterial pressure (MAP), heart rate
(HR) and aortic vascular conductance (AVC) recordings. The LQFM (1.66, 3.32, 6.65 mg/Kg, b.wt.,
i.v.; in 0.1 mL) or vehicle (Tween 2%,i.v.; in 0.1 mL) was infused through the femoral vein cannula.
Results: In anesthetized rats (n=6), infusion of vehicle (Tween 2%) do not promote changes in MAP
(-1 ± 0.2 mmHg), HR (-2 ± 0.8 bpm) and AVC (3 ± 0.5%). Intravenous infusion of LQFM 163 produced
significant hypotension (-13 ± 1.5mmHg) and aortic vasodilation (11 ± 2.5%) and do not promote
changes in HR (-3 ± 5.4bpm) in dose of 6.65 mg/Kg. Conclusion: The prototype LQFM 163 has
potential utility and hypotensive vasodilator, however, further studies are needed to better
characterize cardiovascular changes.
04.022 - EXOGENOUS L-ARGININE REDUCES METALLOPROTEINASES ACTIVITY AND OXIDATIVE
STRESS IN HYPERTENSIVE PATIENTS
VINICIUS PACHECO GARCIA1; HELENA NALY MIGUENS ROCHA1; GUSTAVO MATARUNA DA SILVA1;
IGOR RIBEIRO DA COSTA CARDOSO1; GABRIEL MATHEUS DA SILVA BATISTA1; DANIEL GALINIS VIEIRA
LIMA1; ANTONIO CLAUDIO LUCAS DA NÓBREGA1; LAURO CASQUEIRO VIANNA2; NATÁLIA GALITO
ROCHA1
1.UNIVERSIDADE FEDERAL FLUMINESE, NITEROI, RJ, BRASIL; 2.UNIVERSIDADE DE BRASÍLIA,
BRASÍLIA, DF, BRASIL.
Aim: Defective L-arginine–nitric oxide pathways in hypertension patients are associated with
imbalances in metalloproteinases (MMPs) metabolism and redox homeostasis. We determined the
effects of exogenous L-arginine infusion on endothelial biomarkers in hypertensive patients.
Methods: Blood samples were drawn from seven hypertensive (HT; 45±5yr) and eleven
normotensive men (CT; 37±3yr) before and during intravenous infusion of L-arginine. Serum NO
(NO analyzer), MMP-2 and MMP-9 activities (gelatin zymography), tissue inhibitor of
metalloproteinases-1 (TIMP-1, ELISA) and superoxide dismutase activity (SOD, ELISA) were
measured. Thiobarbituric acid reactive substances (TBARS) were quantified as a proxy of oxidative
stress. Results: At baseline, NO, TIMP-1 and MMP-2 and SOD activities were similar between groups
(P>0.05), while TBARS and MMP-9 were higher in the HT (P=0.02). During L-arginine infusion, NO
increased only in the CT (P=0.01 vs. baseline; P=0.03 vs. HT), while MMPs activities and MMP9/TIMP-1 ratio decreased only in HT (P≤0.02). Yet, the increase of NO concentration (infusion
minus baseline) was negatively associated with baseline systolic blood pressure (P=0.04) in HT.
Although L-arginine infusion has normalized TBARS levels in HT (P=0.13), the SOD activity was
higher in CT (P=0.01). Conclusion: Despite impaired L-arginine-NO pathway, exogenous L-arginine
diminishes MMPs activity and MMP-9/TIMP-1 ratio as well as normalizes oxidative stress in
hypertensive patients.
04.023 - HYPOTENSION AND VASODILATASION RESPONSES INDUCED BY THE LQFM 47
NATHALIA ODA AMARAL
Objective: LQFM 47 compound was synthesized from cilostazol compounds. Studies suggest that
cilostazol be used to peripheral vascular diseases treatment. The present study evaluated the
(HR) and aortic vascular conductance (AVC) recordings. The LQFM (0.42, 0.83 and 1.66 mg/Kg,
b.wt., i.v.; in 0.1 mL) or vehicle (Tween 2%, i.v.; in 0.1 mL) was infused through the femoral vein
cannula. Results: In anesthetized rats (n=6), infusion of vehicle (Tween 2%) do not promote
changes in MAP (-3 ± 1.4 mmHg), HR (-4 ± 1.5 bpm) and AVC (3 ± 2.0 %). Intravenous infusion of
LQFM 47 produced significant hypotension (-20 ± 4.9 mmHg) and aortic vasodilation (12 ± 8.6%)
and do not promote changes in HR (20 ± 9.8 bpm) in dose of 1.66 mg/Kg. Conclusion: Taken
together, the result demonstrated that LQFM 47 is an compound that induces hypotension and
aortic vasodilatation in normotensive rats. However further studies are required to identify the
mechanisms involved in the vasodilation and hypotensive responses.
04.024 - CARDIOVASCULAR RESPONSES INDUCED BY INTRAVENOUS ADMINISTRATION OF LQFM
96 IN RATS
CASSIA MARIZ DA SILVA
UNIVERSIDADE FEDERAL DE GOIÁS (UFG), APARECIDA DE GOIANIA, GO, BRASIL.
Objective: LQFM 96 was synthesized from cilostazol compound. Studies suggest that cilostazol is
a vasodilator drug used to prevent cerebrovascular accident. The present study evaluated the
(HR) and aortic vascular conductance (AVC) recordings. The LQFM 96 (1.66, 3.32, 6.65 and 13.33
mg/Kg, b.wt., i.v.; in 0.1 mL) or vehicle (Tween 2%, i.v.; in 0.1 mL) was infused through the
femoral vein cannula. Results: In anesthetized rats (n=6), infusion of vehicle (Tween 2%) do not
promote changes in MAP (-1 ± 0.5 mmHg), HR (-1 ± 0.7 bpm) and AVC (4 ± 1.0 %). Intravenous
infusion of LQFM 96 produced significant hypotension (-12 ± 2.4 and 23 ± 4.4 mmHg) and aortic
vasodilation (19 ± 4.0% and 17 ± 3,0%) in doses of 6.65 and 13.33 mg/Kg, respectively. No changes
were observed in HR after LQFM 96 administration (1 ± 1.1 bpm and -10 ± 10.0 bpm). Conclusion:
The results demonstrated that LQFM 96 induced hypotension and aortic vasodilatation in
normotensive rats. New experiments are required to identify the mechanisms involved in the
vasodilation and hypotensive responses.
04.025 - DIFFERENCES IN CA2+ HANDLING IN RAT ATRIAL AND VENTRICULAR MYOCYTES
9
JAIR TRAPÉ GOULART; JOSÉ WM BASSANI; ROSANA ALMADA BASSANI
CENTRO DE ENGENHARIA BIOMÉDICA E DEP. DE ENGENHARIA BIOMÉDICA/FEEC, UNIVERSIDADE
ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL.
The aim of this study was to investigate atrium vs. ventricle differences in Ca 2+ transients and
fluxes, and in the contribution of different systems to Ca2+ transport. Adult rat atrial and
ventricular myocytes were isolated after cardiac perfusion with collagenase (CEUA/IB/UNICAMP,
No. 3658-1). Cells were electrically stimulated under perfusion with modified Tyrode´s solution
at 23 °C. Cytosolic Ca2+ concentration ([Ca2+]i) was measured with indo-1. Ca2+ fluxes were
estimated according to Bassani et al. (Gen Physiol Biophys 2012; 31:401) to calculate the relative
contribution to [Ca 2+]i decline of the sarcoplasmic reticulum (SR) Ca 2+ uptake, the Na+/Ca2+
exchanger (NCX) and the slow remaining transporters, and the fractional SR Ca 2+ release during a
twitch (FR). Data were compared with Mann-Whitney or Student´s t test. Although twitch Ca2+
transient amplitude and FR were not statistically different, atrial myocytes showed faster twitch
[Ca2+]i decline (half time: 102±9 vs. 148±10 ms; N= 16; p<0.01) and greater SR Ca2+ content (164±5
vs. 135±6 µM; p<0.01). The SR contribution to Ca2+ transport was greater in atria (93.0±0.6 vs.
82.7±1.9%; p<0.01), whereas NCX contribution was larger in the ventricle (15.4±1.9 vs. 4.8±0.4%;
p<0.01). It thus seems that the contribution of transsarcolemmal Ca 2+ fluxes to excitationcontraction coupling and relaxation is greater in ventricular cells, probably due to the greater
surface-to-volume ratio conferred by the presence of T-tubules, which are absent in atria.
04.026 - ACUTE AORTIC REGURGITATION AND THE AUTONOMIC MODULATION TO THE HEART
PALOMA GRAZIELE BITTENCOURT DA SILVA1; ANA CAROLINA MIEKO OMOTO1; RUBENS FAZAN2;
JULIANA IRANI FRATUCCI DE GOBBI1
1.DEPT. OF PHYSIOLOGY, INSTITUTE OF BIOSCIENCE - UNESP BOTUCATU, BOTUCATU, SP, BRASIL;
2.DEPT. OF PHYSIOLOGY, SCHOOL OF MEDICINE, USP, RIBEIRAO PRETO, SP, BRASIL.
Aortic regurgitation (AR) is a diastolic reflux of blood to the left ventricle. Acute AR has a poor
prognostic and it’s caused by bacterial endocarditis. There’s still missing data in literature about
how the autonomic nervous system acts in this situation. So we evaluated hemodinamic
parameters, autonomic modulation, heart rate (HR) variability and baroreflex sensitivity (BRS) in
AR rats. For this purpose, Wistar rats (250g-280g) were submitted to sham or AR surgery, done by
retrograde puncture of the leaflets. After a week, all the animals received a catheter into the
femoral artery (to register hemodynamic data), and another into the jugular vein (drug
administration). The autonomic parameters were evaluated through double blockage with
atropine (3mg/kg) and atenolol (8mg/kg). Intrinsic heart rate (IHR) was determined after two
consecutives days of double blockage.The analisys of HR variability were done by CardioSeries
program. AR rats presented lower diastolic pressure (70 ± 6 vs sham 99 ± 5 mmHg) and there
weren’t any other diferences in hemodinamics and autonomics parameters. IHR were similar
between the groups (364 ± 11 vs sham 419 ± 23 bpm), and so was HR variability. SBR was lower in
AR acute rats (1,13 ± 0,11 vs sham 1,64 ±0,14 ,ms/mmHg). Acute AR did not alter hemodinamic
parameters, however determined a decrease in spontaneous barorroflex sensitivity, sugesting a
damage in this defense mechanism to the heart.
04.027 - ETHANOL WITHDRAWAL-INDUCED HIPERTENSION AND OXIDATIVE STRESS: ROLE OF
AT1 RECEPTORS
NATÁLIA ALMEIDA GONZAGA; CARLOS RENATO TIRAPELLI
Ethanol withdrawal activates the renin-angiotensin system and systemic concentrations of
angiotensin II. The aim of our study was to evaluate the role of AT 1 on ethanol withdrawal-induced
cardiovascular disorders. Male Wistar rats (250g) were divided into 6 groups: Control: animals
received water ad libitum for 21 days and daily gavage (DG) of vehicle (CV) or losartan (LST10mg/kg/day - CL); Ethanol: animals were treated with an ethanol 9% (v/v) for 21 days and DG of
vehicle (EV) or LST (EL); Ethanol withdrawal: animals were treated in the same way that ethanol
group for 21 days and after that the ethanol was removed and the animals received water ad
libitum until the 23rd, and during ethanol withdrawal period (48h) DG of vehicle (EWV) or LST
(EWL) (CEUA Protocol: 11.1.1432.53.5). To analyze the results we used one-way ANOVA followed
by Tukey (*p<0.05). Ethanol withdrawal induced an increase of systolic blood pressure (mmHg;
n=10) that was prevented by LST (C/V=121±1; CL=122±0.8; EV=121±1 EL=122±0.8; EWV=154±1*;
EWL=122±1). In the plasma, ethanol withdrawal induced an increase of TBARS (nmol/ml; n=9) that
was prevented by LST (CV=10±1; CL=12±1; EV=13±1; EL=14±1; EWV=20±2*; EWL=9±1). In the aorta,
ethanol withdrawal induced an increase in O2- (RLU/mg protein; n=8) that was prevented by LST
(CV=248±19; CL=214±18; EV=228±18; EL=214±21; EWV=655±51*; EWL=199±24). Aortic catalase
activity was not altered by ethanol withdrawal. Ethanol withdrawal increases the systolic blood
pressure and oxidative stress and these responses are mediated by AT1 receptors.
04.028 - INVOLVEMENT OF THE MEDIAN PREOPTIC NUCLEUS IN TONIC CONTROL OF THE
RENOVASCULAR HYPERTENSION
ALINE ANDRADE MOURAO1; MARINA CONCEIÇÃO DOS SANTOS MOREIRA1; ARYANNE BATISTA SOARES
DE MELO1; GRAZIELA TORRES BLANCH2; ANDRE HENRIQUE FREIRIA-OLIVEIRA1; GUSTAVO RODRIGUES
PEDRINO1
1.UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL; 2.PONTIFÍCIA UNIVERSIDADE CATÓLICA
DE GOIÁS, GOIANIA, GO, BRASIL.
Renovascular hypertension is directly related to sympathetic hyperactivation. Recently, our
laboratory demonstrated the participation of the Median Preoptic Nucleus (MnPO) in tonic control
of sympathetic activity in normotensive and spontaneously hypertensive rats. However whether
this nucleus participates of the hypertension maintenance in the renovascular model remains to
be clarified. The present study sought to determine the effect of the MnPO inhibition in the
renovascular hypertension. Male Wistar rats (150-180g) were submitted to the implantation of a
silver clip around the left renal artery to induce 2-kidney 1-clip renovascular hypertension. Five
weeks later, the rats were instrumented for mean arterial pressure (MAP), heart rate (HR) and
renal sympathetic nerve activity (RSNA) records and for nanoinjections of saline (NaCl; 150 mM)
and muscimol (GABAA agonist; 4 mM) into the MnPO. In 2K1C rats (n=6) systolic arterial pressure
increased five weeks after the implantation of the silver clip, when compared to normotensive
rats (208.76±15.09 mmHg vs. 141.77±14.72 mmHg; p<0.05). In 2K1C rats, the muscimol
nanoinjections (n=6) promote significantly decreases of MAP (-17.9±3.4 mmHg), HR (-13.3±5.6
bpm) and RSNA (-13.8±2.5 %) when compared to the saline nanoinjections (n=6; MAP -0.2±0.6
mmHg, HR 2.6±0.9 bpm and RSNA -0.8±1.1 %). In summary, these results demonstrate the MnPO
participation in autonomic and cardiovascular tonic control in renovascular hypertension.
04.029 - CHRONIC ADMINISTRATION OF CATALASE INHIBITOR DECREASES SYMPATHETIC
MODULATION IN RATS WITH RENOVASCULAR HYPERTENSION.
MARIANA RUIZ LAUAR; LAURIVAL ANTONIO DE LUCA JR; PATRICIA MARIA DE PAULA; DEBORA SIMÕES
ALMEIDA COLOMBARI; EDUARDO COLOMBARI; CARINA A.F. ANDRADE; JOSÉ VANDERLEI MENANI
DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, DENTISTRY SCHOOL, SÃO PAULO STATE
UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL.
Recent data have shown that chronic subcutaneous (sc) administration of the catalase inhibitor 3amino-1,2,4-triazol (ATZ) reduces blood pressure (BP) in 2-kidneys, 1-clip (2K1C) hypertensive
rats. In the present study, we analyzed the autonomic modulation of cardiovascular function in
2K1C hypertensive rats treated with chronic sc injection of ATZ. Male Holtzman rats (initial weight
150-180 g, n=7-11/group) received a silver clip around the left renal artery to generate 2K1C
hypertension. After 6 weeks of the surgery, rats were treated with ATZ or saline for 7 days before
the recording of arterial pressure and the analysis of systolic blood pressure (SBP) and pulse
interval (PI) variability using power spectral analysis. Baseline MAP was increased in 2K1C rats
compared to sham (207 ± 9, vs. 100 ± 2 mmHg, respectively). ATZ (600 mg/kg/day) reduced the
low frequency (LF) of SBP (3.3 ± 0.5, vs. saline 7.6 ± 1.5 mmHg2) and LF of PI (14.6 ± 3, vs. saline:
31 ± 3 normalized units) in 2K1C hypertensive rats. ATZ increased high frequency (HF) of PI (85 ±
3, vs. saline: 68 ± 3 normalized units) and improved the sympathovagal balance calculated for
LF/HF ratio of PI variability (0.2 ± 0.05, vs. saline: 0.52 ± 0.09). The results suggest that the
treatment with ATZ reduces BP in 2K1C hypertensive rats by reducing the modulation of
sympathetic activity.
10
04.030 - INCREASED EXPRESSION OF MACROPHAGE MIGRATION INHIBITORY FACTOR IN THE
NUCLEUS OF SOLITARY TRACT ATTENUATES THE RENOVASCULAR HYPERTENSION
RAFAELA MOREIRA BARBOSA1; GUILHERME F.F. SPERETTA1; ANDRE HENRIQUE FREIRIA-OLIVEIRA2;
HONGWEI LI3; COLIN SUMNERS4; JOSÉ VANDERLEI MENANI1; EDUARDO COLOMBARI1; DEBORA SIMÕES
ALMEIDA COLOMBARI1
1.DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, SÃO PAULO STATE
UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL; 2.CENTER FOR NEUROSCIENCE AND
CARDIOVASCULAR PHYSIOLOGY, FEDERAL UNIVERSITY OF GOIÁS, GOIÂNIA, GO, BRASIL;
3.SOUTHERN MEDICAL UNIVERSITY, GUANGZHOU, CHINA; 4.DEPARTMENT OF PHYSIOLOGY AND
FUNCTIONAL GENOMICS, COLLEGE OF MEDICINE, UNIVERSITY OF FLORIDA, GAINESVILLE, ESTADOS
UNIDOS.
Macrophage migration inhibitory factor (MIF) is an intracellular inhibitory regulator of the actions
of angiotensin II (ANG II) in the central nervous system. ANG II actions at the nucleus of the solitary
tract (NTS) are important for the maintenance of hypertension in renovascular hypertensive 2kidney, 1-clip (2K1C). Therefore, we tested if increased expression of MIF in the NTS of 2K1C rats
could alter the maintenance of hypertension and improve baroreflex function in these animals.
MIF expression in the NTS was increased by AAV2-CBA-MIF microinjection and AAV2-CBA-eGFP
served as a control vector. Vectors were injected 3 weeks after renal clip placement. MIF
injections in the NTS attenuated the hypertension in 2K1C rats from 2 weeks after viral
transduction until the end of the experiment (4 weeks after viral transduction), (2K1C-MIF: 145 ±
7, vs. 2K1C-eGFP: 176 ± 9 mmHg). MIF into the NTS also improved the reflex bradycardia (2K1CMIF slope: -1.4 ± 0.3, vs. 2K1C-eGPF slope: -0.41 ± 0.3 bpm/mmHg) and reflex tachycardia (2K1CMIF slope: -4.7 ± 0.6, vs. 2K1C-eGPF slope -1.7 ± 0.3 bpm/mmHg). MIF overexpressed in the NTS
in normotensive rats produced no change in arterial pressure or baroreflex function. These results
suggest that increased expression of MIF in the NTS attenuates the hypertension and improves
baroreflex function in 2K1C rats, possibly through anti-ANG II action.
HF:91±4; HF+PYR:87±2 mmHg] were not altered in HF rats or after PYR administration. However,
both the tachycardic (Control:-2.9±0.3; HF:-1.2±0.1; HF+PYR:-3.7±0.5 bpm/mmHg) and
bradycardic (Control:-2.1±0.2; HF:-1.4±0.1; HF+PYR:-1.9±0.3 bpm/mmHg) baroreflex sensitivity
were attenuated in HF rats, but improved by PYR. Thus, the long-term administration of PYR
reduced RSNA and improved the baroreflex control of HR.
04.031 - ANGIOTENSIN TYPE 2 RECEPTOR ACTIVATION WITHIN THE ROSTRAL VENTROLATERAL
MEDULLA REDUCES BLOOD PRESSURE IN 2K1C HYPERTENSIVE RATS
JESSICA L.B. MIURA1; RAFAELA MOREIRA BARBOSA1; JOSÉ VANDERLEI MENANI1; COLIN SUMNERS2;
EDUARDO COLOMBARI1; DEBORA SIMÕES ALMEIDA COLOMBARI1
1.DEPARTMENT OF PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, SÃO PAULO STATE
UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL; 2.DEPARTMENT OF PHYSIOLOGY AND
FUNCTIONAL GENOMICS, COLLEGE OF MEDICINE, UNIVERSITY OF FLORIDA, GAINESVILLE, ESTADOS
UNIDOS.
Previous studies have demonstrated that intracerebroventricular infusion of the selective
angiotensin II type 2 receptor (AT2R) agonist (Compound 21; C21) reduced mean arterial pressure
(MAP) in normotensive rats (Gao et al, Am; J. Hypert., 24(6): 724, 2014). The rostral ventrolateral
medulla (RVLM) is an important structure for regulating sympathetic outflow and blood pressure.
AT2R overexpression in the RVLM reduces MAP in normotensive rats (Gao et al, Hypertension,
51(2): 521, 2008). Renovascular hypertensive 2-kidney, 1-clip (2K1C) rats exhibit increased
activity of the renin-angiotensin system and pronounced sympathoexcitation. Therefore, we
tested if bilateral microinjection of C21 into the RVLM of 2K1C rats could induce cardiovascular
changes. C21 (200 nL of 100 µM) was injected bilaterally 6 weeks after renal clip insertion. MAP
and heart rate (HR) were measured in freely moving sham (n = 5) and 2K1C rats (n = 5). C21
microinjections in the RVLM decreased the MAP in 2K1C rats (before-C21: 169 ± 9, vs. after-C21:
152 ± 8 mmHg), but not in sham rats (before-C21: 104 ± 3, vs. after-C21: 104 ± 3 mmHg). Similarly,
injection of C21 into the RVLM reduced HR in 2K1C rats (before-C21: 336 ± 15, vs. after-C21: 300
± 6 bpm), but not in sham rats (before-C21: 346 ± 12, vs. after-C21: 332 ± 12 bpm). These results
suggest that AT2R activation in the RVLM is effective in reducing MAP and HR in 2K1C rats.
04.036 - BLOQUEIO DE RECEPTORES AT1 CENTRAIS REDUZ A PRESSÃO ARTERIAL EM ANIMAIS
ALIMENTADOS COM DIETA HIPERLIPÍDICA
JESSICA MATHEUS DE SA; MARIANA DEL ROSSO MELO; GUILHERME F.F. SPERETTA; JOSÉ VANDERLEI
MENANI; EDUARDO COLOMBARI; DEBORA SIMÕES ALMEIDA COLOMBARI
UNESP - UNIVERSIDADE ESTADUAL PAULISTA, ARARAQUARA, SP, BRASIL.
O entendimento da fisiopatologia da hipertensão derivada da obesidade tem sido foco de vários
estudos. A obesidade aumenta a atividade do sistema renina-angiotensina. Neste trabalho
verificamos o efeito do bloqueio de receptores angiotensinérgicos do subtipo AT1 centrais sobre
o aumento da pressão arterial em ratos alimentados com dieta hiperlipídica. Foram utilizados
ratos Holtzman (250-300 g) com cânulas de aço inoxidável implantadas no ventrículo lateral (VL)
alimentados por 6 semanas com dieta hiperlipídica (DH; 26,4% de gorduras; n = 9) ou dieta padrão
(DP; 5,4% de gorduras; n = 5). Após 6 semana de DH ou DP, a artéria femoral foi canulada, e no
dia seguinte a pressão arterial média (PAM) e frequência cardíaca (FC) foram registradas em ratos
não anestesiados. Losartan (100 μg/1 μl – antagonista de receptores AT1) foi injetado no VL. A
PAM basal nos animais HD foi maior do que nos animais DP (PAM: 114 ± 3, vs. DP: 103 ± 2 mmHg,
p<0,05). Vinte minutos após a injeção de losartan no VL a PAM no grupo DH foi reduzida a níveis
semelhantes aos observados no grupo DP (DH: 107 ± 1, vs. DP 105 ± 2 mmHg –p > 0,05).
Angiotensina II no VL não produziu efeito pressor após o tratamento com losartan, mostrando a
eficácia no bloqueio de receptores AT1. Os dados sugerem que receptores AT1 centrais participam
do aumento de PAM observado em ratos alimentados com dieta hiperlipídica por 6 semanas.
04.032 - HIGH FAT-DIET INDUCES MICROGLIA ACTIVATION AND ASTROGLIOSYS IN THE IN THE
NUCLEUS OF THE SOLITARY TRACT IN RATS
GUILHERME FLEURY FINA SPERETTA; MARIANA DEL ROSSO MELO; MIRIAN BASSI; JOSÉ VANDERLEI
MENANI; EDUARDO COLOMBARI; DEBORA SIMÕES ALMEIDA COLOMBARI
UNESP/ARARQUARA, ARARAQUARA, SP, BRASIL.
Neuroinflammation seems to be a mechanism involved in the obesity-induced cardiovascular
disturbers. In the present study, we evaluated the microglia activation and astrogliosys in the in
the nucleus of the solitary tract in HFD feeding rats. Male Holtzman rats (300-320 g, n = 7/group)
were fed with either standard rat chow diet (SD; 5.4% total fat) or HFD (26.4% total fat) for 6
weeks. Coronal sections (30 µm) of the brainstem were sectioned on a cryostat and incubated
with anti Iba-1 and anti-GFAP antibodies to label respectively, microglia and astrocytes. For the
assessment of microglial number, 20 μm z-stacks were counted bilaterally by hand each 120 µm,
in matched representative sections of the NTS. For assessment of GFAP immunoreactivity, images
were converted into greyscale and binary formats and thresholds for black and white balance were
adjusted to the same level in matched representative sections of the NTS. Rats fed with HFD
exhibited higher immunoreactivity for GFAP (162 ± 9 vs SD: 133 ± 3 and 140 ± 5 vs. SD: 119 ± 5
area of GFAP staining, p < 0.05) and higher number of Iba-1 positive cells (23 ± 2 vs. SD: 16 ± 1
and 24 ± 2 vs. SD: 17 ± 1 Iba-1 positive cells number, p < 0.05), in the intermediate NTS and in
the commissural NTS, respectively. These results suggest a participation of microglia and
astrocytes in the chronic inflammation in the NTS that could contribute to the cardiovascular
alterations induced by HFD.
04.033 - RUBPY IS A NITRIC OXIDE-DONOR THAT INHIBITS PLATELET AGGREGATION
TAMY MIDORI BANIN1; ROBERTO SANTANA DA SILVA1; AMRITA AHLUWALIA2; LUSIANE MARIA
BENDHACK1
1.FCFRP/USP, RIBEIRÃO PRETO, SP, BRASIL; 2.WHRI/QMUL, LONDON, REINO UNIDO.
The ruthenium complex RuBPY is promising as a new vasodilator because it causes complete
relaxation of rat aorta, presents hypotensive effect and it is not cytotoxic. The mechanisms by
which, RuBPY causes relaxation were investigated but it is still unknown if it releases nitrite or
nitric oxide (NO). Therefore, this study aimed to evaluate if the compound RuBPY releases nitrite
or NO to induce vasodilation and if it inhibits platelet aggregation. NO• formation in absence of
tissue was measured in a sealed glass reaction chamber containing buffer at pH 7.4 and pH 5.0
and sodium nitrite (from 1 to 1000 μM) or RuBPY (1-100 μM), bubbled with nitrogen gas to simulate
ischemia. The NO• concentration was measured by ozone chemiluminescence and the platelet
aggregation was assessed by Light Transmission Aggregation. Human volunteers’ blood was used
and platelet rich plasma was considered equivalent to no aggregation, and platelet poor plasma
100% aggregation. NO production induced by NaNO2 was greater in acid pH with the highest
concentration used (10 mM). RuBPY produced higher amount of NO than the 100 µM NaNO2 in both
acid and basic pH. The platelet aggregation was accessed in response to stimulation with ADP and
collagen. Platelets incubation with 100 µM RuBPY inhibited the platelet aggregation. Taken
together, the results showed that RuBPY is an NO donor, that releases NO in a tissue-independent
manner and that it is able to inhibit platelet aggregation.
04.034 - INCREASE IN PARASYMPATHETIC FUNCTION BY ACETYLCHOLINESTERASE INHIBITION
PREVENTS SYMPATHO-EXCITATION AND BAROREFLEX DYSFUNCTION IN HEART FAILURE RATS
RENATA M. LATARO; CARLOS ALBERTO AGUIAR SILVA; DANIEL P MARTINS DIAS; FERNANDA
MACHADO SANTOS-ALMEIDA; RUBENS FAZAN; HELIO CESAR SALGADO
FACULDADE DE MEDICINA DE RIBEIRÃO PRETO, RIBEIRÃO PRETO, SP, BRASIL.
Excessive sympatho-excitation and abnormality of baroreflex sensitivity are hallmarks of heart
failure (HF). We investigated the effects of the increase of parasympathetic function, by the
acetylcholinesterase inhibitor pyridostigmine (PYR), on renal sympathetic nerve activity (RSNA)
and baroreflex function in HF rats. HF was induced by myocardial infarction. PYR was administered
for 4 weeks, per os. After that the animals underwent surgery to implant stainless steel electrodes
around the renal nerve and catheters into the femoral artery and vein. Baroreflex-mediated
changes in RSNA were assessed, in conscious state, by the mean AP (MAP) vs. RSNA curve, after
intravenous administration of phenylephrine and sodium nitroprusside. HF rats showed higher
RSNA that was reduced by PYR (Control:332±31; HF:434±31; HF+PYR:325±13 bursts/min). The
range of RSNA (Control:105±1; HF:104±3; HF+PYR:102±1 %max), gain (Control:-1.43±0.1; HF:1.65±0.2; HF+PYR:-1.8±0.1%max/mmHg) and the midpoint of the curve [(MAP50); Control: 87±4;
04.035 - BAROREFLEX ACTIVATION IN CONSCIOUS RATS MODULATES THE JOINT INFLAMMATORY
RESPONSE VIA SYMPATHETIC FUNCTION
FERNANDA BROGNARA1; GABRIEL S BASSI1; JACI AIRTON CASTANIA1; JHIMMY TALBOT1; THIAGO M
CUNHA1; FERNANDO Q CUNHA1; LUIS ULLOA2; ALEXANDRE KANASHIRO1; DANIEL P MARTINS DIAS1;
HELIO CESAR SALGADO1
1.FACULDADE DE MEDICINA DE RIBEIRÃO PRETO - USP, RIBEIRAO PRETO, SP, BRASIL; 2.NEW JERSEY
MEDICAL SCHOOL, NEWARK, ESTADOS UNIDOS.
The baroreflex is a critical physiological mechanism controlling cardiovascular function by
modulating both the sympathetic and parasympathetic activities. Here, we report that electrical
activation of the baroreflex attenuates joint inflammation in experimental arthritis induced by
the administration of zymosan into the femorotibial cavity. Baroreflex activation combined with
lumbar sympathectomy, adrenalectomy, celiac subdiaphragmatic vagotomy or splenectomy
dissected the mechanisms involved in the inflammatory modulation, highlighting the role played
by sympathetic inhibition in the attenuation of joint inflammation. From the immunological
standpoint, baroreflex activation attenuates neutrophil migration and the synovial levels of
inflammatory cytokines including TNF, IL-1β and IL-6, but does not affect the levels of the antiinflammatory cytokine IL-10. The anti-inflammatory effects of the baroreflex system are not
mediated by IL-10, the vagus nerve, adrenal glands or the spleen, but by the inhibition of the
sympathetic drive to the knee. These results reveal a novel physiological neuronal network
controlling peripheral local inflammation.
THEME 05 – GASTROINTESTINAL PHYSIOLOGY
05.001 - NITRIC OXIDE PARTICIPATES IN THE GASTRIC EMPTYING DECREASE INDUCED ACUTE
PHYSICAL EXERCISE IN RATS
ANA KAROLINA MARTINS CAVALCANTE1; ROMMULO CELLY LIMA SIQUEIRA2; LORENA BEZERRA
MARTINS1; ARMÊNIO AGUIAR DOS SANTOS2; MOISES TOLENTINO BENTO-SILVA3
1.INTA, SOBRAL, CE, BRASIL; 2.UFC, FORTALEZA, CE, BRASIL; 3.UFPI, TERESINA, PI, BRASIL.
Introduction: The acute physical exercise (APE) slows gastric emptying (GE), dependent on the
basic acid balance. Objective: To evaluate the role of the pathway of NO on the GE fluid in rats
submitted to APE. Methods: Male rats (230-280g) sedentary (SED) or APE as previously described
by (J Appl Physiol 116(9),2014). Divided in: SED, APE-2.5%, pc, APE-5%, pc, Sedentary+L-Name
(SED+L-NAME) and APE-5%+L-NAME (APE-5%+L- NAME). We evaluated the levels of corticosterone
[cortico], lactate [Lac] and NO. After 18h fasting rats were submitted or not to APE. The rats
SED+L-NAME and APE-5%+L-NAME were pre-treated (30min) to (L-NAME 10 mg / kg, ip) 10min after
all groups evaluated for GE, according to (J Physiol,131: 452-62,1956). Average data expressed ±
EPM compared by ANOVA and Tukey test. Results: We observed a significant decrease in the GE
values of APE-2.5% rats and APE-5% compared to the SED(64,7±3,1 and 69,5±1,9 vs 52,1±3,9%).
Regarding SED rats, APE-5%rats significantly increased Cortico values(13,6±2,5 vs.
28,5±1,9mg/dL), Lac(1,9±0,1 vs 6,5±0,6mmol/dL), and NO(8,1±0,7 vs 28,0±4,2nmol/mg/protein).
The APE-5%+L-NAME group had its inhibition of GE prevented significantly compared to APE-5%
(69,5±1,9 vs 55,5±4,6%). We did not observe differences between the SED+L-NAME and APE-5%+LNAME. Conclusion: The APE decreases GE and increases Lac. This phenomenon seems to be
involved in stress as the route of NO, where there was an increase [NO], and prevention of
decrease in the GE by pretreatment with L-NAME.
05.002 - PRETREATMENT WITH GLUTAMINE PREVENTS LIVER INJURY RESULTING FROM
INTESTINAL ISCHEMIA AND REPERFUSION IN RATS
NORMA ANAIR POSSA MARRONI1; RENATA HARTMANN2; FRANCIELLI LICKS2; ELIZÂNGELA SCHEMITT2;
JOSIELI COLARES1; ROSA MARIA MOURA1; GILMARA ZABOT3; HENRIQUE FILLMANN3
1.UNIVERSIDADE LUTERANA DO BRASIL, PORTO ALEGRE, RS, BRASIL; 2.UNIVERSIDADE FEDERAL DO
RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL; 3.PONTIFÍCIA UNIVERSIDADE CATÓLICA DO RIO
GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL.
Background: The intestinal ischemia-reperfusion (I/R) can cause cellular damage to the tissue and
distant organs such as the liver occuring the generation of free radicals and the nitric oxide. Aim:
Evaluate the effects of glutamine (gln) in an animal model of I/R. Material and methods: Twenty
male Wistar rats were divided into four groups: Sham operated (SO), Glutamine+Sham operated
(G+SO), ischemia-reperfusion (I/R), Glutamine+ischemia-reperfusion (G+I/R). The rats were
subjected to occlusion of the superior mesenteric artery for 30 min and 15 min of reperfusion.
The gln (25 mg/kg/day) was administered 24 and 48 h before I/R. Liver injuries were determined
for oxidative stress using lipid peroxidation (LPO), levels of nitric oxide metabolites (NO) and the
expression of the enzyme inducible nitric oxide synthase (iNOS) by western blot. Statistical
analysis used was ANOVA - Student-Newman-Keuls (mean±SEM) significant at p<0.05. Results:
Animals treated with gln showed a significant reduced the LPO levels (SO: 0.16±0.01, G+SO:
0.20±0.02, I/R: 0.45±0.03; G+I/R: 0.24±0.02) compared to animals of I/R group. The NO levels
showed a decrease in the treated group (SO: 5.4±0.8; G+SO: 6.9±0.8; I/R: 15.6±2.7; G+I/R:
6.1±1.1) and also to reduced expression of iNOS (SO: 1.2±0.3; G+SO: 0.9±0.30; I/R: 2.5±0.10;
G+I/R: 1.8±0.1) compared to I/R group. Conclusion: These results suggest that pretreatment with
gln improves liver injury after intestinal I/R injury in rats.
05.003 - N-ACETYLCYSTEINE MODULATES ANGIOGENESIS, VASODILATION AND DNA DAMAGE IN
STOMACH OF PORTAL HYPERTENSIVE RATS
NORMA ANAIR POSSA MARRONI1; FRANCIELLI LICKS2; RENATA HARTMANN 2; ELIZÂNGELA SCHEMITT2;
CAMILA MARQUES3; JOSIELI COLARES1; MARIANA SOARES2; JULIANA SILVA1
1.UNIVERSIDADE LUTERANA DO BRASIL, PORTO ALEGRE, RS, BRASIL; 2.UNIVERSIDADE FEDERAL DO
RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL; 3.INSTITUTO DE TECNOLOGIA DO PARANÁ,
CURITIBA, PR, BRASIL.
Background and Aims: Portal Hypertension is associated with the development of a collateral
circulation due to increase on portal pressure. Our aim was evaluate the antioxidant effect of NAcetylcysteine (NAC) on the stomach of portal hypertensive rats. Methods: Animals were divided
in four groups (n=6): Sham-operated (SO), SO+NAC, Partial portal vein ligation (PPVL) and
PPVL+NAC. NAC (10 mg/kg ip) was administered daily for 7 days starting 8 days after surgery. We
performed evaluation of portal pressure, expression of endothelial nitric oxide synthase (eNOS),
vascular endothelial growth factor (VEGF) and nitrotyrosine (NTT) by immunohistochemistry and
Western blot, and assessed DNA damage in blood sample by comet assay. Variance analysis
(ANOVA) and Student-Newman Keuls were used for multiple analysis, and the level of significance
was 5% (P < 0.05). For comet assay, we used the Kolmogorov -Smirnov test. Results: The PPVL
group showed an increase in portal pressure, eNOS, VEGF and NTT expression. NAC was able to
decrease the values of portal pressure and all the proteins evaluated. Furthermore, NAC was able
to modulate DNA damage in PPVL+NAC animals. Conclusions: In conclusion, NAC was able to
minimize gastric vasodilation, evaluated by eNOS, angiogenisis, evaluated by VEGF, oxidative
stress evaluated by NTT and protected gastric mucosa also from DNA damage. We suggest NAC is
able to protect stomach from the alterations developed by the PPVL procedure.
05.004 - MELATONIN ATTENUATING OXIDATIVE DAMAGE IN HEPATIC CARCINOGENESIS INDUCED
BY DIETHYLNITROSAMINE IN RATS
ANDREA CRISTIANE JANZ MOREIRA
UFRGS/HCPA, PORTO ALEGRE, RS, BRASIL.
This study developed HCC using diethylnitrosamine (DEN) in rats and proposed the treatment with
melatonin (MLT). We evaluated oxidative stress in lipids, protein and DNA, comet assay liver (CA),
biochemical and histological analysis. Material and Methods: 28 male Wistar rats, 145g were
divided into: (I)Control, (II)HCC–DEN 50mg/kg ip, (III)MLT5-DEN 50mg/kg ip+melatonin at 5th week
(IV)MLT12-DEN 50mg/kg ip+melatonin at 12th week. MLT (20mg/L) was administrated in water.
Blood was collected to evaluate transaminases (AST and ALT), gamma glutamyl transferase (GGT)
and alkaline phosphatase (AP) (U/L). Liver samples were removed for CA and histological analysis.
Statistical analysis:Tuckey test with p<0,05. Results: MLT prolongs the survival in III and IV groups
vs II group (p=.0001). MLT reduced damage (DI) and frequency (DF) index in DNA (DI:I=95+27;
II=339+56; III=259+42; IV=119+17; DF: I=74+17; II=99+2; III=92+5; IV=60+5). AST(I)107+7 (II)236+69
(III)165+59 (IV)143+27; ALT(I)54±15 (II)144+36 (III)102+36; (IV)=95+18 GGT (I)1+0.9 (II)85+45
(III)43+33 (IV)25+14; AP(I)111+50 (II)279+91 (III)215+98 (IV)212+46; group II showed significant
increase compared to groups I, III and IV. Liver histology of II group was characterized by
pseudoacinar and trabecular growth pattern. We concluded that chronic and intermittent
exposure model to carcinogenic agents develops HCC in rats. Melatonin improved hepatic function
and reduced damage on DNA, resulting an increase in OS in rats with HCC.
THEME 06 – CELULAR PHYSIOLOGY
06.001 - EVALUATION OF OXIDATIVE STRESS INDUCTION BY PUTRESCINE IN LIVER OF WISTAR
RATS
LETÍCIA CRISTINA GONÇALVES1; LETÍCIA DE SOUSA FRANCO1; GUILHERME ANDRADE ALVES1; RAÍSSA
FERRAZ CURTOLO1; FRANCO DANI CAMPOS PEREIRA2; MARIA APARECIDA MARIN-MORALES2;
GRASIELA DIAS DE CAMPOS SEVERI-AGUIAR1
1.FHO- UNIARARARAS, RIO CLARO, SP, BRASIL; 2.UNESP, RIO CLARO, SP, BRASIL.
The putrescine acts as an intracellular messenger and is involved in homeostatic mechanisms.
When in imbalance is able to induce some diseases like cancer. In order to assess the ability of
putrescine at inducing changes in levels of oxidative stress, 24 Wistar rats were exposed by gavage
for 56 consecutive days at three different concentrations (T1- 231.5 mg/kg; T2- 138.9 mg/kg and
T3- 46.3 mg/kg), and the results were compared with the control group (drinking water). The
sulfhydryl groups (-SH), the level of lipid peroxidation (TBARS/MDA), glutathione (GSH) and the
activity of catalase (CAT), superoxide dismutase (SOD) and glutathione-S-transferase (GST) were
quantification. The statistical analysys were submitted to the tests t and pos hoc, Man Whitney
test (p> 0.05). In all groups exhibited a significant decrease was observed for GST (Co- 0.055 ±
0.004; T1- 0.049 ± 0.003; T2- 0.042 ± 0.001; T3- 0.048 ± 0.005) and increase in TBARS (Co- 244.00
± 27.93; T1- 370.00 ± 48.30; T2- 276.00 ± 21.91; T3- 286.67 ± 23.09). In T1 and T3 groups, levels
of SOD (Co- 0.362 ± 0.022; T1- 0.246 ± 0.067; T2- 0.340 ± 0.074; T3- 0.134 ± 0.027) decreased
significantly and GSH increased significantly (Co -0.009 ± 0.002; T1- 0.046 ± 0.005; T2- 0.012 ±
0.005; T3- 0.042 ± 0.005). However, not observed significant changes for the sulfhydryl groups (SH) as well as to the activity of the enzyme CAT. These results show that putrescine caused lipid
peroxidation, and reduced levels of antioxidant enzymes, featuring induction of hepatic oxidative
stress.
06.002 - THE EFFECT OF STEVIOSIDE ON THE TRANSPORT OF OLEIC ACID IN RAT ADIPOCYTES
SUELLEN HARUMI OKUMA; MARIA IDA BONINI RAVANELLI; SÍLVIO CLÁUDIO DA COSTA; CECÍLIA EDNA
MAREZE DA COSTA; KELLEN BRUNALDI
UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGÁ, PR, BRASIL.
This study aimed to evaluate the effect of stevia glycoside stevioside (Stv) on the transport of
oleic acid (OA) in isolated adipocytes of the epididymal fat of rats obtained by collagenase
digestion. The fluorescent pH probe BCECF was trapped in the cytoplasm and measurements
performed with a fluorimeter. Adipocytes were initially incubated with Stv (10 mM) for 4 min. Stv
did not affect BCECF fluorescence as it has no ionizable groups. The subsequent addition of OA
(1.3 mM) to cells caused a fast drop in fluorescence (t 1/2 < 2 sec), which corresponded to the
release of H+ by OA that had reached the inner leaflet of the plasma membrane. The kinetic of
BCECF fluorescence change reflected the combined steps of OA binding and diffusion across the
membrane. Addition of OA to cells in the absence of Stv (control experiment) produced similar
reduction in BCECF fluorescence. However, none of those data informed about Stv partitioning
into the lipid bilayer. Therefore, the Stv analog steviolbioside (Stvbio), which has a carboxyl
group, was tested. Stvbio (1.6 mM) caused a fast drop in BCECF fluorescence (t 1/2 < 2s), which
reflected the binding and diffusion of Stvbio across the membrane, with the delivery of H + to the
cytoplasm. We concluded that the Stv did not affect the transport of OA in isolated rat adipocytes.
Stv probably partitioned into the lipid bilayer as inferred from the data with Stvbio.
06.003 - CAN TEMPERATURE SYNCHRONIZE PERIPHERAL CLOCKS OF ECTOTHERMIC ANIMALS?
MARCOS RODRIGO JERONIMO DA COSTA; BRUNO CÉSAR RIBEIRO RAMOS; ANA MARIA DE LAURO
CASTRUCCI
INSTITUTO DE BIOCIÊNCIAS - USP, SAO PAULO, SP, BRASIL.
Thermo-sensation allowed the animals to monitor environmental temperature changes, in order
to prevent harmful effects on vital physiological processes. Temperature is a major synchronizing
agent of peripheral clocks, through the regulation of clock genes expression. In this aspect,
thermo-TRP (transient receptor potential) channels are of upmost importance. Using quantitative
PCR we demonstrated the expression of the following trp genes in the cell line ZEM-2S of the
teleost Danio rerio: trpV1, trpC6, trpA1a, trpA1b, trpM2, trpV4, trpM4a, trpM4b/c and trpM5.
To analyze the participation of TRP channels in the modulation of clock genes by temperature,
pulses of 2.5 and 5o C above the maintenance temperature (28o C) were applied to ZEM-2S cells
kept in constant darkness (DD). Our results indicate that the expression of per1, per2, cry1a and
cry1b was not affected by 2.5o C pulse, whereas the 5o C pulse increased the expression of per2
and cry1a, same clock genes reported to be induced by light. Accordingly, it has been established
that TrpV1 is essential for Danio rerio to detect temperatures above 32o C, what may explain why
the increase of just 2.5o C was ineffective. We are presently investigating the nature of the TRP
channel involved in the response of clock genes to 5o C pulse.
THEME 07 – COMPARATIVE PHYSIOLOGY
07.001 - PULMONARY MECHANICS AND THE WORK OF BREATHING IN CAIMAN YACARE
MICHELLE REICHERT; WILLIAM K MILSOM
UNIVERSITY OF BRITISH COLUMBIA, VANCOUVER, CANADA.
The pulmonary mechanics and work of breathing of an animal reflect the structure and complexity
of the lungs and the chest cavity that surrounds the lungs. Within Class Reptilia, there is large
variation in body wall architecture and lung structure, ranging from simple (snake) to complex
(crocodilian). We measured static and dynamic pulmonary mechanics in the Yacare caiman
(Caiman yacare) and found that total system mechanics primarily reflected the mechanics of the
chest wall, but not to the same degree as other reptiles. The lungs of the caiman were complex
and less compliant than other reptiles and also contributed significantly to overall stiffness. While
total system compliance decreased with increasing breathing frequency, it did not decrease with
11
increasing tidal volume, which was surprising given the heavily keratinized nature of the chest
wall. The work of breathing, however, increased with both tidal volume and breathing frequency
and the majority of the work performed during breathing was to overcome the elastic forces of
the chest wall resisting inflation. As tidal volume and breathing frequency increased, however,
the work performed to overcome resistive forces in the airways increased proportionately. While
the pulmonary mechanics of the caiman more closely resembled that of other reptiles than that
of mammals overall, they begin to take on the characteristics of the mammalian respiratory
system.
07.002 - EFFECTS OF POSITIONS ON MECHANICS OF VENTILATION IN PHRYNOPS GEOFFROANUS
PEDRO HENRIQUE TREVIZAN BAU; WILFRIED KLEIN; TÁBATA ELISE FERREIRA CORDEIRO
The reptilian respiratory system shows great diversity in lung morphology and respiratory
mechanisms, which are strongly influenced by morphological differences. Despite sharing
respiratory system characteristics with other reptiles, testudines have a unique trait: the ribs are
attached to the shell, which could influence breathing mechanics. The aim of this study was to
measure, for the first time, the static and dynamic compliance of the intact respiratory system
(CT) in P. geoffroanus (n=4) in 4 positions (supine, prone, legs and head retracted into the shell,
and submerged), as well as in isolated lungs (C L). Measurements of static compliance were
performed by stepwise injection/removal of known air volumes. Dynamic compliance was
determined by pump ventilation of the respiratory system at known volumes and frequencies and
measuring the resulting pressure changes. Static CL was greater when compared to static CT
(43.6±7.1 mL.cmH2O-1.kg-1). Comparing submerged to other positions, the values were
significantly greater (22.1±1.1 mL.cmH2O-1.kg-1) than prone (7.7±1.0 mL.cmH2O-1.kg-1), and legs
and head retracted (6.6±0.7 mL.cmH2O-1.kg-1) and the “Residual lung volume/Maximum lung
volume” was lower (20.7±3.2%) because of hydrostatic pressure. This relationship could explain
the greater inspiratory reserve and compliance of respiratory system. This result is being
confirmed in dynamic compliance experiments where CT tend to be equal in high frequencies
between positions but differing at low frequencies.
07.003 - STATIC AND DYNAMIC BREATHING MECHANICS IN PODOCNEMIS UNIFILIS
TABATA ELISE FERREIRA CORDEIRO; WILFRIED KLEIN; PEDRO HENRIQUE TREVIZAN BAU
USP, RIBEIRÃO PRETO, SP, BRASIL; ; .
The relationship between pressure and volume of a respiratory system can be described by its
compliance (C). In reptiles, increase in the complexity of lungs leads to increased C. Therefore,
this study aims to understand the influence of the elastic forces of the respiratory system as well
as the lungs in Podocnemis unifilis (N = 5) Static and dynamic C of the intact respiratory system
(CT) were determined with animals in supine and prone positions, with their legs retracted into
the carapace and while floating in water. Isolated lungs also had their static and dynamic C
determined (CL). Resting lung volume (VRL) in animals under the water was lower values (43.6 ±
10) whereas in maximum volume (VML) at the same position was the highest between the different
conditions (221.9 ± 7.1). These results can be explained by hydrostatic pressure exerted over the
lungs and led to lower ratio of VRL/ VML. Static CT was greatest in submerged animals (CT = 41.3
± 7.8 mL.cmH2O-1.kg-1) and animals with restrained limbs showed were least complacent
respiratory system (CT = 19.2 ± 5.8 mL.cmH2O-1.kg-1), due to the rigid shell limiting lung
expansion. Dynamic compliance decreased with increasing frequency in all different position and
was not significantly affected by variations in volume. This work was the first that investigated
CT and CL in Podocnemis unifilis and with positions observed in nature.
07.004 - VARIAÇÔES SAZONAIS NO METABOLISMO DO SIRI CALLINECTES SAPIDUS NA LAGOA DE
TRAMANDAÍ (IMBÉ/RS)
JORGE FELIPE ARGENTA MODEL; JORDANA TRES SANTOS; ANAPAULA SOMMER VINAGRE; MÁRCIA
TRAPP
UFRGS, PORTO ALEGRE, RS, BRASIL.
O objetivo do trabalho foi verificar as alterações metabólicas sazonais na população de C. sapidus
da Lagoa de Tramandaí/RS. Os animais foram coletados (março de 2013 a dezembro de 2014) na
Lagoa de Tramandaí/RS, permaneceram em aquários com condições controladas e foram
alimentados diariamente com lulas por 15 dias. Os siris foram crioanestesiados para a coleta de
hemolinfa e de tecidos (hepatopâncreas, músculos da mandíbula e do quelípede, brânquias
anteriores e posteriores e coração). As concentrações hemolinfáticas de glicose, triglicerídeos,
colesterol e proteínas totais foram determinadas usando kits comerciais e expressas em mg/dL.
Após hidrólise ácida, o glicogênio nos tecidos foi determinado e expresso como mg /g de tecido.
Na hemolinfa, as concentrações de triglicerídeos aumentaram do verão (12,9 ± 2,2) em relação
ao outono (21,5 ± 2,2); os níveis de colesterol aumentaram do verão (9,8 ± 1,5) comparado ao
outono (26,6 ± 2,5) (p<0,05); a concentração de proteínas reduziu do outono (4,1 ± 0,3) comparado
ao inverno (2,1 ± 0,8) (p<0,05), enquanto a glicemia não variou. Os níveis de glicogênio nos
músculos da mandíbula (0,5 ± 0,1), do quelípede (0,08 ± 0,02) e do coração (1,79 ± 0,4) reduziram
no verão (p<0,05). Nos demais tecidos não houve diferença significativa. Esses resultados
demonstram a presença de variações sazonais no metabolismo de C. sapidus.
07.005 - EFEITOS METABÓLICOS DA ADRENALINA NO CARANGUEJO NEOHELICE GRANULATA
ANAPAULA SOMMER VINAGRE; JORGE FELIPE ARGENTA MODEL
O objetivo foi investigar os efeitos da adrenalina sobre o metabolismo do caranguejo Neohelice
granulata. Caranguejos machos foram mantidos em aquários, em condições controladas, e
alimentados com carne bovina (dieta RP) ou arroz cozido (dieta RC) durante 15 dias. Para a curva
de tempo, os animais foram crioanestesiados e amostras de hepatopâncreas, brânquias anteriores
(BA) e brânquias posteriores (BP) foram incubadas, a 25ºC com Ringer ou adrenalina 2,5 mM e
ácido ascórbico (AA) 0,1 M por 15, 30 ou 60 min. Após a incubação foi determinada a glicose dos
meios. Para a curva de dose, os tecidos foram incubados com adrenalina nas doses 10-7M, 10-6M,
10-5M, 10-4M e 10-3M e AA por 30min. Após a incubação foi determinada a concentração de glicose
e glicerol dos meios e a de glicogênio dos tecidos. Tanto nas BP como nas BA de animais RC
incubados com Ringer, a liberação de glicose para o meio foi máxima aos 30 min, enquanto no
hepatopâncreas não foram verificadas alterações significativas. Os valores de glicogênio e de
glicerol não sofreram alterações significativas em relação às doses de adrenalina. Os valores de
glicose nos meios diminuíram na presença de adrenalina 10-3M no hepatopâncreas e 10-6M nas
BP. Esses resultados sugerem uma ação da adrenalina sobre a oxidação da glicose, o que ainda
precisa ser investigado.
THEME 08 – RESPIRATORY PHYSIOLOGY
08.001 - PURINERGIC RECEPTORS BLOCKADE IN THE RETROTRAPEZOID NUCLEUS ATTENUATES
THE RESPIRATORY RESPONSE TO CHEMOREFLEX IN AWAKE RATS
BARBARA FALQUETTO BARNA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA
Central and peripheral chemoreceptors sense changes in CO2/H+ and/or O2 and communicate this
information to cardiorespiratory centers to regulate breathing and sympathetic outflow to ensure
adequate ventilation-perfusion matching in tissues. Despite the importance of this reflex, the
neurotransmitter basis for integration of this information in the brainstem is unclear. Therefore,
our main goal was to elucidate whether purinergic signaling contributes to central and peripheral
chemoreceptor activation in the RTN in unanesthetized rats.
Male Wistar rats with bilateral stainless steel cannula implanted into the RTN were used. Bilateral
injection of PPADS (a P2 receptor blocker - 5 mM - 100 nl) into the RTN attenuated the excitatory
effect of ATP on minute ventilation (Ve: 839 ± 115, vs. ATP 3808 ± 1047 ml/kg/min) and the
increase in Ve produced by hypercapnia (7% CO2) (1027.5 ± 89.1, vs. saline: 1694.7 ± 188.7
ml/kg/min) and hypoxia (8% O2) (650.9 ± 151, vs. saline: 1146.8 ± 330.4 ml/kg/min). The bilateral
blockade of the P2Y1 receptors (MRS2179 - 100 M - 100 nl) into the RTN region did not change
the excitatory effect of ATP nor the increase in Ve produced by hypercapnia or hypoxia in
conscious unrestrained rats.
These results support that i) the cardiorespiratory effects elicited by ATP are depended on P2
receptors but not P2Y1; ii) the hypercapnia and hypoxia ventilatory responses are mediated by
purinergic signaling in conscious unrestrained rats
08.002 - THE PRESENCE OF ACTIVE EXPIRATION IN RAT IN VIVO AND IN VITRO PREPARATIONS
SARAH JENKIN
UNIVERSITY OF BRITISH COLUMBIA, VANCOUVER, CANADA.
Mammalian expiration is typically passive at rest, yet when respiratory drive increases expiration
becomes active (AE). This study compares the presence of active expiration in rats using in vivo
and in vitro preparations. AE can be elicited in vivo when unanesthetized rats are under high
respiratory drive (8% inspired CO2 or higher). AE is elicited more easily when unanesthetized,
post-vagotomy (6% inspired CO2). AE cannot be elicited in vivo under anesthetic with or without
the vagi intact. These data suggest that vagal feedback and anesthesia (urethane) inhibit active
expiration, with the effects of the anesthetic overriding the effects of vagotomy. AE cannot be
elicited from the in vitro brainstem-spinal cord preparation (with or without the pons) even though
there is no vagal feedback. This is most likely due to the inability to provide sufficient respiratory
drive to the preparation. Upcoming research will investigate the role of the vagus nerve, the pons
and hypercapnia on the appearance of AE using the in situ working heart-brainstem preparation.
08.003 - EFFECTS OF TYROSINE KINASE INHIBITOR DASATINIB IN PULMONARY AND
EXTRAPULMONARY ACUTE LUNG INJURY AND HUMAN NEUTROPHILS
AMANDA DE LACERDA CLEVELARIO; JOHNATHAS DUTRA SILVA; GISELE PENA DE OLIVEIRA; GISELE
PADILHA; DEBORA GONÇALVES XISTO; PATRICIA RIEKEN MACEDO ROCCO
UFRJ, RIO DE JANEIRO, RJ, BRASIL.
RATIONALE: Evidence suggests that tyrosine-kinase inhibitors may attenuate lung inflammation
and fibrosis in acute respiratory distress syndrome (ARDS). Since the pathophysiology of ARDS
differs depending on the type of primary insult, we hypothesized that dasatinib, a tyrosine-kinase
inhibitor, might act differently depending on ARDS etiology and dose.
METHODS: 64 C57BL/6 mice were randomly assigned across eight groups. In the Cp group, saline
solution was instilled intratracheally (i.e.) while Cexp received saline intraperitoneally (i.p.).
ARDS mice received Escherichia coli lipopolysaccharide i.t. (ARDSp) or i.p. (ARDSexp). At 24h,
animals received dasatinib (10, 20 or 30 mg/kg) or vehicle (1% dimethyl sulfoxide) by oral gavage.
After 24h, lung mechanics and histology were assessed. Mortality and lung edema were evaluated
in other groups of animals treated with dasatinib 10 mg/kg twice daily by oral gavage for 7 weeks.
RESULTS: Static lung elastance and pulmonary damage increased similarly after ARDS induction
and were reduced by dasatinib 30 mg/kg, with no significant changes with 10 and 20 mg/kg
regardless of ARDS model. Dasatinib decreased mortality, body weight and pulmonary edema in
ARDSp compared to ARDSexp.
CONCLUSION: Dasatinib improved lung morphofunction regardless of ARDS etiology, but beneficial
effects were observed only with a higher dose. However, chronic use of low-dose dasatinib
reduced mortality rate in pulmonary ARDS.
08.004 - THE ROLE OF METABOTROPIC GLUTAMATE RECEPTORS IN THE LATERAL
HYPOTHALAMUS AND PERIFORNICAL AREA IN VENTILATORY RESPONSE TO HYPERCAPNIA IN
RATS DURING WAKEFULNESS
LAISA TAIS CABRAL RODRIGUES1; JOSÉ DE ANCHIETA DE CASTRO HORTA JÚNIOR2; LUCIANE HELENA
GARGAGLIONI BATALHAO3; MIRELA BARROS DIAS1
1.UNESP- UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE MESQUITA FILHO - DEPARTAMENTO DE
FISIOLOGIA, BOTUCATU, SP, BRASIL; 2.UNESP - UNIVERSIDADE ESTADUAL PAULISTA JÚLIO DE
MESQUITA FILHO, DEPARTAMENTO DE ANATOMIA, BOTUCATU, SP, BRASIL; 3.UNESP - FCAV DEPARTAMENTO DE MORFOLOGIA E FISIOLOGIA ANIMAL, JABOTICABAL, SP, BRASIL.
Introduction: Evidence suggests there is a diurnal variation of central chemoreflex, and it has
been suggested that orexinergic neurons of lateral hypothalamus and area perifornical (LH/PFA)
have a key role in the modulation of the central chemosensitivity in a vigilance-state manner, but
the mechanisms involved have not been elucidated so far. Objective: To evaluate the importance
of metabotropic glutamate receptors, located in the LH/PFA, in the hypercapnic ventilatory
response in unanesthetized rats, during wakefulness and sleep. Methods: We measured pulmonary
ventilation (VE), and body temperature (Tb) together with electroencephalogram (EEG) and
electromyogram (EMG) before and after microinjecting alpha-methyl-4-carboxyphenylglycine
(MCPG; 100mM) or vehicle (aCSF) into the LH/PFA of male Wistar rats, followed by 40 min of
hypercapnia exposure (7% CO₂). Nissl staining procedure was used to evaluate, based on the
cytoarchitecture of the LH/PFA region, the location of injections. Results: MCPG injected into
LH/PFA decreased the ventilatory response to hypercapnia due to a lower respiratory frequency
in awake rats, compared with the control group. Conclusion: These data suggest that the
glutamate acting on metabotropic receptors in the LH/PFA contributes to the hyperventilation
induced by hypercapnia during wakefulness.
08.005 - GAP JUNCTIONS IN THE ROSTRAL MEDULLARY RAPHE MODULATE THE HYPERCAPNIC
VENTILATORY RESPONSE IN UNANESTHETIZED RATS
LAISA TAIS CABRAL RODRIGUES1; RAIMUNDO DA SILVA SOARES JÚNIOR1; FELIPE FIORIN ZAMAI1; JOSÉ
DE ANCHIETA DE CASTRO HORTA JÚNIOR2; LUCIANE HELENA GARGAGLIONI BATALHAO3; MIRELA
BARROS DIAS1
1.UNESP - UNIVERSIDADE ESTADUAL PAULISTA JULIO DE MESQUITA FILHO, DEPARTAMENTO DE
FISIOLOGIA, BOTUCATU, SP, BRASIL; 2.UNESP - UNIVERSIDADE ESTADUAL PAULISTA JULIO DE
MESQUITA FILHO, DEPARTAMENTO DE ANATOMIA, BOTUCATU, SP, BRASIL; 3.UNESP - FCAV,
DEPARTAMENTO DE MORFOLOGIA E FISIOLOGIA ANIMAL, JABOTICABAL, SP, BRASIL.
Introduction: It has been demonstrated that Medullary Raphe (MR) plays an important role in
central chemoreception. Gap junction proteins are expressed in neurons of putative CO2chemosensitive brainstem regions, including the MR, but it is not known whether gap junctions,
located specifically in the rostral MR, contributes to the ventilatory response to hypercapnia.
Objective: The present study was designed to investigate the involvement of gap junctions in the
CO2 ventilatory response in unanesthetized adult rats. Methods: Pulmonary ventilation (measured
by body plethysmography) and body temperature (Tb) were determined before and after injection
of carbenoxolone (CARB; 3mM/100nL) or vehicle (sterile 0,9% saline) into the rostral MR of Wistar
rats. These measurements were made during 30 min in normocapnic conditions followed by 40
min of 7% CO2 exposure. Nissl staining procedure was used to evaluate, based on the
cytoarchitecture of the MR region, the location of injections. Results: Gap junction blockade in
MR neurons augmented the ventilatory response to hypercapnia (1762.707±285,57mL/kg/min for
the control group and 2063.89±194.91mL/kg/min for 3mM CARB, P<0.01) due to an increased tidal
volume. Conclusion: These results suggest that gap junctions in the rostral MR exert an inhibitory
modulation of the hypercapnic ventilatory response of adult rats.
08.006 - ACUTE HYPOXIA INCREASES PROINFLAMMATORY MARKERS IN CENTRAL AUTONOMIC
NUCLEI
TALITA DE MELO E SILVA; REINALDO CORREIA SILVA; LAIALI JURDI CHAAR; VAGNER ROBERTO
ANTUNES; NIELS OLSEN CÂMARA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA
UNIVERSITY OF SÃO PAULO, SAO PAULO, SP, BRASIL; ; ; ; ; ; .
Prolonged and continuous exposure of mammals to a low oxygen environment (chronic hypoxia)
elicits remarkable physiological adjustments. Among them are altered gene expression, increased
peripheral chemosensitivity, enhanced respiratory drive and sympathoexcitation. The current
study examines the hypothesis that acute hypoxia initiates an immune response elicited by an
increased expression of inflammatory markers. Male Wistar rats (280-320g; 6-8/group, CEUA:
163/2012) were subjected to acute hypoxia (AH: 8% O2) or normoxia (N: 21% O2) for 3 hours. AH
increased the proinflammatory cytokine IL-6 in the heart (90.8 ± 20 vs. N: 1.8 ± 0.7), lung
(15.9±3.1 vs. N: 3.3±1.4), kidney (12.1±3.2 vs. N: 1.2±0.2) and liver (2.9±0.5 vs. N: 1.0±0.06) as
well as in brain areas such as the rostral ventrolateral medulla (RVLM) (2.43±0.29 vs. N: 1.0±0.12)
and paraventricular nucleus of the hypothalamus (PVH) (1.8±0.2 vs. N: 0.8±0.1). AH also increased
IL-1 β within the RVLM (3.4±0.3 vs. N: 0.8±0.06) and PVH (1.69±0.3 vs. N: 0.9±0.1), TNF within
the RVLM (2.37±0.7 vs. N: 0.7 ± 0.04), CD86 within the PVH (1.43±0.1 vs. N: 0.9±0.06), LY6C within
the RVLM (8.6±2.6 vs. N: 1.0±0.26) and MMP9 within the RVLM (2.3±0.5 vs. N: 0.82±0.09) and PVH
12
(1.26±0.3 vs. N: 0.5±0.05). Taking together these results suggest that inflammatory mechanisms
may be involved in the response to chemoreceptors activation during AH.
08.007 - ACUTE HYPOXIA ACTIVATES HYPOTHALAMIC PARAVENTRICULAR NUCLEUSPROJECTING CATECHOLAMINERGIC NEURONS IN THE C1 REGION
TALITA DE MELO E SILVA; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA
UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL.
C1 cells reside in the rostral and intermediate portions of the ventrolateral medulla (RVLM) and
can be activated by hypoxia. These neurons regulate the hypothalamic pituitary axis via direct
projections to the hypothalamic paraventricular nucleus (PVH) and regulate the autonomic
nervous system via projections to sympathetic and parasympathetic preganglionic neurons. Based
on the various effects attributed to the C1 cells and what is currently known of their synaptic
inputs, our hypothesis is that acute hypoxia (AH) activates PVH projecting catecholaminergic
neurons in the RVLM. Anterograde tracer, Phaseolus vulgaris leucoagglutinin (PHA-L) was
unilaterally injected into the RVLM and a retrograde tracer Cholera toxin B (CTB) was unilaterally
injected into the PVH region. After ten days, male Wistar rats that received CTB injection into
the PVH were subjected to AH (8% O2, balanced with N2) or normoxia (21% O2) for 3 hours. Acute
hypoxia significantly increased Fos immunoreactivity in the C1 region (68.5 ± 2.0), and half of the
RVLM cells activated are catecholaminergic (35.5 ± 2.3). We observed that (30 ± 4%) of the PVH
projecting RVLM cells that were activated by AH were also C1 cells. The presence of varicosities
containing PHA-L in PVH region was also observed. The present results suggest that
catecholaminergic C1-PVH projection is hypoxia sensitive and the pathway between these two
important brain areas can certainly be one more piece in the complex puzzle of neural control of
autonomic regulation during hypoxia.
08.008 - HYPERCAPNIA-SENSITIVE CHEMORECEPTORS NEURONS OF RETROTRAPEZOID NUCLEUS
INNERVATE THE KOLLIKER-FUSE REGION IN RATS
JOSIANE DO NASCIMENTO SILVA; TALITA DE MELO E SILVA; ROSÉLIA DOS SANTOS DAMASCENO; ANA
CAROLINA T TAKAKURA; THIAGO S MOREIRA
USP, SÃO PAULO, SP, BRASIL.
The Kölliker-Fuse (KF) region, located in the dorsolateral pons, receives several projections from
brainstem areas involved in respiratory regulation, including the chemoreceptors neurons within
the retrotrapezoid nucleus (RTN). We hypothesized that the KF region could contribute to central
respiratory chemoreflex activation. To test this hypothesis, we used immunohistochemistry
experiments in male Wistar rats (250-330 g, CEUA: 008/2012). After iontophoretic injection of the
anterograde tracer biotinylated dextran amine (BDA) in RTN, most (35 ± 6%) BDA-labeled terminals
in the KF region were immunoreactive for vesicular glutamate transporter-2 (VGLUT2). Awake rats
were exposed to hypercapnia one week after iontophoretic injection of the retrograde tracer
FluoroGold (FG) into the KF. Hypercapnia-activated neurons were identified by the presence of
Fos-immunoreactive (Fos-ir) nuclei. Fos-ir RTN neurons retrogradely labeled with FG were
detected only in hypercapnia-treated rats. In RTN, 84 ± 6% of Fos-ir neurons with projections to
RTN (FG-immunoreactive) were not tyrosine hydroxylase. In conclusion, the RTN-KF neuronal
connection is probably one of several brainstem pathways through which the RTN neurons activate
breathing.
08.009 - THERAPEUTIC EFFECTS OF DENDRIMER-METHYPREDNISOLONE CONJUGATE IN AN
EXPERIMENTAL MODEL OF ACUTE LUNG INJURY
ROBERTA ESPIRITO SANTO CORREIA; JOHNATHAS DUTRA SILVA; SABRINA V MARTINI; PATRICIA
RIEKEN MACEDO ROCCO; MARCELO MARCOS MORALES
This study investigated the effects of dendrimer-methyprednisolone conjugated in an
experimental models of acute respiratory distress syndrome (ARDS). In BALB/c mice, ARDS was
induced by intratracheal (ARDSp) or intraperitoneal (ARDSexp) injection of E.coli LPS. In control
groups, animals received saline (Cp, Cexp). 24 hours after ARDS induction, groups were divided
receiving saline (SAL, iv); Methyprednisolone (MP, iv) or intratracheal instilations of dendrimermethyprednisolone conjugated (D-MP). 24 hours after the treatment, survival rate, pulmonary
mechanics, lung histology and inflammatory mediators were analyzed. The ARDSexp-SAL group
had a survival of 80%, MP or D-MP improved survival in ARDSexp-SAL animais (8%). Lung elastance
was increased in ARDS animals, the treatment with MP or D-MP attenuated these functional
changes. In ARDS animals, lung histology presented neutrophil infiltration, alveolar collapse,
interstitial oedema, and increase in the amount of collagen and elastic fibers in the alveolar septa.
Additionally, levels of IL (interleukin)1-b, KC (murine homolog of IL-8) and vascular endothelial
growth factor (VEGF) were higher in ARDS groups. MP and D-MP treatment attenuated these
changes, however, in ARDSexp-D-MP the treatment significantly reduced the collapsed areas, the
neutrophil infiltration and attenuated the increase of cellularity and inflammatory mediators. The
treatments improved survival, prevented pulmonary mechanics deterioration, minimized
histological changes and inflammatory mediators in these experimental models of ARDS.
08.010 - CHARACTERIZATION OF EMPHYSEMA MOUSE MODEL INDUCED BY MULTIPLE
INSTILLATIONS OF ELASTASE
MILENA VASCONCELLOS DE OLIVEIRA; NAZARETH DE NOVAES ROCHA; GISELE PADILHA; LIGIA DE
ALBUQUERQUE MAIA; DEBORA GONÇALVES XISTO; SORAIA CARVALHO ABREU; PATRICIA RIEKEN
MACEDO ROCCO
IBCCF-UFRJ, RIO DE JANEIRO, RJ, BRASIL.
Rationale: Although there are several therapeutic strategies for pulmonary emphysema, none is
able to prevent disease progression. This study sought to characterize a model of emphysema
induced by multiple instillations of elastase through periodic analyses of inflammation and
cardiovascular function.
Methods: 48 male C57BL/6 mice were randomly assigned into 2 groups. Emphysema (ELA) animals
received 1, 2, 3 or 4 intratracheal instillations of elastase (PPE, 0.2 UI) with a 1-week interval
between them. Controls (C) received saline using the same protocol. Results: After instillation 1,
the percentage of mononuclear cells in the lung parenchyma increased. After instillation 2, the
fraction of hyperinflated alveoli and mean linear intercept increased, and the elastic fiber content
of the lung parenchyma was reduced. After instillation 3, percentage of neutrophils in the lung
and collagen fiber content in the lung parenchyma and airways increased, and lung static
elastance was reduced. After instillation 4, the percentage of macrophages with M1 phenotype in
the lung, levels of cytokines and growth factors, and collagen fiber content in blood vessels were
increased, and pulmonary arterial hypertension developed.
Conclusion: These findings suggest that the initial phase of emphysema is characterized by an
inflammatory process, whereas its late phase is marked by impairment of pulmonary and
cardiovascular function.
08.011 - THERAPEUTIC EFFECTS OF BOSUTINIB, A TYROSINE KINASE INHIBITOR, ON LUNG
MORPHOFUNCTION AND INFLAMMATION IN EXPERIMENTAL SILICOSIS
PRISCILA DE JESUS CARNEIRO; AMANDA DE LACERDA CLEVELARIO; GISELE PADILHA; FERNANDA
FERREIRA CRUZ; NAZARETH DE NOVAES ROCHA; PATRICIA RIEKEN MACEDO ROCCO
UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL.
Rationale: Silicosis is an occupational lung disease for which no effective treatment exists. We
hypothesized that bosutinib, a tyrosine kinase inhibitor, would attenuate pulmonary fibrosis,
ameliorate inflammatory responses, and improve lung function in experimental silicosis. For this
purpose, we investigated the potential efficacy of bosutinib in the treatment of experimental
silica-induced lung fibrosis.
Methods: Silicosis was induced in C57BL/6 mice (n=32) by intratracheal administration of silica
particles (20 mg/50µl saline); a control group received saline alone. After 15 days, when the
disease was established, animals were randomly assigned to receive DMSO or bosutinib (1 mg/kg
dose in 0.1 ml of DMSO) by oral gavage, twice daily for 14 days. At day 30, lung mechanics (airway
resistance, viscoelastic pressure and static elastance) and morphometry, pulmonary inflammation
(total and differential cell count in alveolar septa), and amount of collagen fibers were measured.
Results: Bosutinib improved lung mechanics (p<0.05) and reduced fractional area of alveolar
collapse, size and number of granulomas, amount of collagen fibers, and number of macrophages
in alveolar septa and granuloma.
Conclusion: In this model of silicosis, bosutinib effectively reduced lung inflammation and fibrosis,
thus improving lung mechanics.
08.012 - GLUCOCORTICOIDS INFLUENCE IN ACUTE LUNG INJURY IN SEPTIC RATS
ERIKA KRISTINA INCERPI GARCIA; ROSELI SONCINI; SILVIA GRACIELA RUGINSK
., ., MG, BRASIL.
In Acute Respiratory Distress Syndrome (ARDS) and Acute Lung Injury (ALI), a down regulation of
systemic inflammation mediated by glucocorticoid receptor (GR) is essential to improve survival.
We investigate the dynamic interaction between agonist and inhibitor of glucocorticoids (GC) on
corticosterone (CCT) plasma levels and immunoreactive area for GR in rat lung with LPA-induced
CLP. We use male Wistar rats (250-300 g) divided into groups Naïve, Sham and CLP (n = 8 / group).
The Naïve group did not undergo the procedure or treatment. Sham group had the exposed cecum
and was pretreated with saline. The CLP group had bonded and perforated cecum and divided
into three groups according to the treatments one hour before surgery: CLP sal (NaCl 0.9% saline
i.p.); CLP met (metyrapone 50 mg.kg-1 i.p.); CLP dex (dexamethasone 0.5 mg.kg-1 i.p.). After 24
hours, the CLP sal had increased plasma CCT and no change in immunoreactivity for GR. The CLP
met showed reduced levels of CCT, decreased cytoplasmic and total immunoreactivity for GR and
increased nuclear, and increased risk of death. The CLP dex showed reduced levels of CCT,
increased nuclear immunoreactivity for GR and decreased risk of death. We concluded that there
was GR resistance in animals pre-treated with sal; there was increased GR sensitivity in the pretreated with met and dex. However, met did not prevent mortality due to reduced supply of CCT
to tissues.
08.013 - GLIMEPIRIDE DOES NOT EXHIBIT CHANGES ON RESPIRATORY RESISTANCES IN NONOBESE MICE: A PRELIMINARY STUDY
MELISSA TEIXEIRA GOULART; CECÍLIA DA COSTA DALÉCIO; MAYCON DANIEL DIAS; MARINA DE FÁTIMA
VENÂNCIO; ROSELI SONCINI
UNIVERSIDADE FEDERAL DE ALFENAS, ALFENAS, MG, BRASIL.
Glimepiride is considered a low-potency insulin secretagogue and is used on glycaemic control.
Literature points an association between insulin and development/severity of asthma, meanly
withlung resistance. We evaluated the glimepiride on airway and tissue resistances, and on
collagen and elastic fibers in non-obese mice.
Nine Swiss mice (w=45±0.7g) were divided in: control group, treated withsaline (n=4); or glim
group,treated withsaline+glimiperide(0.1mg/Kg; n=5). All animals received the solutions by
gavage during 21 days. The glycaemia was performedand after, the animalswere tracheostomized
and ventilated with a ventilator. Airway resistance and tissue damping were investigated after
lung aerosolizationof saline and methacholine (100mg/kg). Lungs were collected and prepared for
morphometric analysis to collagen and elastic fibers. Glycaemia did not changein any group
(134±15mg/dL). Also, we didn´t identify changes in the respiratory parameters. Only parenchyma
collagen fibers percentage was reduced in the group glim in comparison to control (9.4±1%
vs4.3±0.6%, P<0.005).
Glimepiride does not show changes in the respiratory function in non-obese mice, however, the
collagen fibers were reduced. It is known that there are interactionsof the smooth musclesof the
airwaysand thetissuematrix,and we believe that new protocols will run in non-obese and obese
mice in order to elucidate some questions of this preliminary study.
08.014 - PRE-INSPIRATORY ACTIVITY EVOKED BY RETROTRAPEZOID NUCLEUS (RTN)
STIMULATION IS DEPENDENT ON THE PONTINE KÖLLIKER-FUSE (KF) REGION INTEGRITY
ELVIS VIANA DE LUCENA1; ANA CAROLINA T TAKAKURA2; THIAGO S MOREIRA1
1.DEPARTMENT OF PHYSIOLOGY AND BIOPHYSICS, INSTITUTE OF BIOMEDICAL SCIENCE, UNIVERSITY
OF SÃO PAULO, SÃO PAULO, SP, BRASIL; 2.DEPARTMENT OF PHARMACOLOGY, INSTITUTE OF
BIOMEDICAL SCIENCE, UNIVERSITY OF SÃO PAULO, SÃO PAULO, SP, BRASIL.
Pre-inspiratory activity underlies tongue muscle contraction and expansion of the upper airway,
causing a decrease in airway resistance. The pontine Kölliker-Fuse nucleus (KF) has established
functions in the regulation of upper airway patency. We hypothesized that the KF region could
contribute to hypoglossal motor activity to central respiratory chemoreflex activation. To test this
hypothesis, we performed electrophysiological experiments in adult urethane-anesthetized and
vagotomized male Wistar rats (290-330 g, CEUA: 008/2012). Hypercapnia (10% CO2) increased
diaphragm (DiaEMG) and genioglossal (GGEMG) activity and elicited abdominal (AbdEMG) muscle
activity. Bilateral injection of muscimol (GABA-A agonist; 2mM) into the KF region eliminated the
rise in GGEMG (6 ± 4 vs. saline: 47 ± 9%) and reduced the increase in DiaEMG (25 ± 6 vs. saline: 43
± 8%) and AbdEMG (213 ± 23 vs. saline: 335 ± 18%) produced by hypercapnia. The blockade of the
KF region were able to eliminate the GGEMG (2 ± 2%), but only reduced the DiaEMG (12 ± 2%) and
AbdEMG (111 ± 13%) induced by NMDA (1 mM) injection into the RTN. Our data suggest that the
KF neurons could integrate and modulate several aspects of breathing during chemoreceptor
activation.
08.015 - EFFECTS OF DISINHIBITION OF RTN/PFRG NEURONS ON THE TACHYPNEIC RESPONSE
INDUCED BY L-GLUTAMATE
EDUARDO VIEIRA LEMES; DANIEL BRESEGHELLO ZOCCAL
SÃO PAULO STATE UNIVERSITY, ARARAQUARA, SP, BRASIL.
The retrotrapezoid nucleus/parafacial respiratory group (RTN/pFRG) is described as an important
area for central chemoreception and for the generation of active expiration. Experimental
evidence suggests that the RTN/pFRG neurons are under a tonic inhibitory control. In the present
study, we tested the possibility that the inhibitory mechanisms of RTN/pFRG may modulate the
amplitude of inspiratory response to microinjections of L-glutamate in this region. Using arteriallyperfused decerebrated in situ preparations of juvenile male Holtzman rats (60-70g; n=4), we
recorded phrenic nerve (PN) activity and performed unilateral microinjections of L-glutamate
(100mM, 50nL) in the RTN/pFRG before and after microinjections of bicuculline and strychnine
(50μM, 50 nL). L-Glu microinjections in the RTN/pFRG increased PN frequency (ΔPN freq: 41 ± 9
%; P<0.05), but not amplitude (ΔPN amp: 6 ± 7 %). After RTN/pFRG disinhibition, the magnitude
of changes in PN frequency (ΔPN freq: 39 ± 6 %) and amplitude (ΔPN amp: 7 ± 3 %) did not change.
Our data indicate that the tachypneic response to L-glutamate microinjections is not influenced
by the inhibitory mechanisms of the RTN/pFRG.
THEME 09 – ENDOCRINE & METABOLISM
09.001 - UROCORTIN 2 ENHANCES SKELETAL MUSCLE MASS AND STIMULATES AMPC/PKA/CREB
AND AKT/FOXO1 SIGNALING PATHWAYS IN RODENTS
NATALIA LAUTHERBACH ENNES DA SILVA1; DAWIT ALBIEIRO PINHEIRO GONÇALVES1; SILVIA DE
PAULA GOMES2; NEUZA MARIA ZANON1; LUIZ CARLOS CARVALHO NAVEGANTES1; ISIS DO CARMO
KETTELHUT3
1.RIBEIRÃO PRETO MEDICAL SCHOOL - UNIVERSITY OF SÃO PAULO/ DEPARTMENT OF PHYSIOLOGY,
RIBEIRAO PRETO, SP, BRASIL; 2.RIBEIRÃO PRETO MEDICAL SCHOOL - UNIVERSITY OF SÃO PAULO/
DEPARTMENT OF BIOCHEMISTRY/IMMUNOLOGY, RIBEIRAO PRETO, SP, BRASIL; 3.RIBEIRÃO PRETO
MEDICAL SCHOOL - UNIVERSITY OF SÃO PAULO/ DEPARTMENTS OF BIOCHEMISTRY AND
PHYSIOLOGY, RIBEIRAO PRETO, SP, BRASIL.
Introduction: Recent studies reported that Urocortin 2 (Ucn2) presents anti-atrophic and
hypertrophic effects in skeletal muscle of rodents. However, the intracellular mechanisms
responsible for these findings remain unclear. This work investigates the role of Ucn2 in the
signaling pathways involved in the control of protein metabolism in skeletal muscle. Methods: EDL
and soleus skeletal muscles were isolated from normal Wistar rats and C57Bl6 mice and incubated
with Ucn2 (10-8M, 7.10-8M, 10-7M, 5.10-7M) for 2h to investigate the rates of protein degradation
by measuring the tyrosine release. C2C12 myotubes were incubated with Ucn2 (10nM) for 5min,
30min and 3 hours. In order to superexpress this peptide, mouse Ucn2 plasmid was transfected in
vivo in tibialis anterior muscle of normal C57Bl6 mice for 7 and 14 days. Then, tibialis anterior
and C2C12 were processed to determine the phosphorylation levels of PKA substrates, CREB, Akt,
13
Foxo1 and ERK1/2 by Western Blot. Results: Ucn2 decreased overall proteolysis in vitro in soleus
and EDL muscles. The super expression of Ucn2 in vivo induced hypertrophy in tibialis anterior (14
days) and increased the phosphorylation levels of CREB, Akt, Foxo1 and ERK1/2 (7 days). Ucn2 in
vitro enhanced phospho-PKA substrates in myotubes. Conclusion: These results suggest that the
anti-proteolytic and hypertrophic effects of Ucn2 are mediated by the activation of
AMPc/PKA/CREB, Akt/Foxo1 and ERK1/2 signaling pathways.
09.002 - STRUCTURAL ORGANIZATION OF THE PLACENTA IN LATE PREGNANCY OF RATS FED
50% CALORIC RESTRICTION
ROSANA CATISTI; KATIA ESTEFANIA DE CARVALHO; LARISSA RUELLA DE ASSIS; HÉRCULES JONAS
REBELATO; MARCELO AUGUSTO MARRETO ESQUISATTO
Placental weight is highly correlated with birth weight and therefore, survival and growth rate of
the newborn mammal. Placental growth is maximal in the first half of gestation suggesting that
placental size and even function later in gestation may be substantially influenced by food
restriction in this time. This study investigated whether gestational caloric restriction alters
placenta morphology at end of gestation. Pregnant female Wistar rats were divided in control
group (C, n = 6, basal diet) and restricted group fed 50% of the diet consumed by control (R, n =
6). At day 21 of gestation (21 dG), after anestesia, the placenta were collected and weighed. For
histological analysis were processed fragments of right horn placenta located in the middle region.
It was observed significant decrease in the R group in relation to placenta and fetuses weight.
Morphometric analysis of the placenta regions, the junctional and the labyrinth zone did not
detect size alterations. However, there was a decrease in the number of giant and glycogen cells.
It was observed an increase in placenta glycogen content in R rats. The current data led us to
hypothesize that maternal caloric restriction altered the placental size but its structural
organization were preserved.Study approved by CEUA/UNIARARAS (055/2014).
09.003 - STRUCTURAL ORGANIZATION OF THE LIVER IN LATE PREGNANCY OF RATS FED 50%
CALORIC RESTRICTION
JOYCE FERNANDA CAVENAGHI RADDI; LARISSA RUELLA DE ASSIS; MARCELO AUGUSTO MARRETO
ESQUISATTO; HÉRCULES JONAS REBELATO; RENATA BARBIERI; ROSANA CATISTI
Pregnancy is a state of dynamic changes in metabolism and nutrient utilization. The liver plays an
essential role in maternal metabolic adaptation during late pregnancy. Glucose is metabolized to
supply energy that is used to promote fetal growth and development and that is converted to fetal
liver glycogen to ensure neonatal survival. Despite this well-described metabolic plasticity, the
morphology changes underlying the hepatic adaptation to pregnancy remain unclear. The aim of
this study was to investigate whether 50% caloric restriction diet intake during pregnancy alters
structural organization in female rat liver at day 21 of gestation (21 dG). Pregnant female Wistar
rats were divided in control group (C, n = 6, basal diet) and restricted group fed 50% of the diet
consumed by control (R, n = 6). At 21 dG, after anestesia, the animals were perfused, the liver
collected, weighed and processed by Hematoxylin-Eosin. The R dams tended to have a smaller
liver mass in relation to body weight than C dams. It was observed a decrease in liver glycogen in
R rats. Morphological analysis showed both the liver structure and the organization of hepatocytes
similar between groups. The lobules did not present signals of inflammation, steatosis and fibrosis
in both groups. Sinusoids vessels structure and distribution were similar. We hypothesize that
maternal caloric restriction altered the liver size but not its structural organization. Study
approved by CEUA/UNIARARAS (055/2014).
09.004 - CAMUNDONGOS C57BL6 TRANSGÊNICOS PARA A APOCIII HUMANA APRESENTAM
REDUZIDA GLICOGENÓLISE E GLICÓLISE ADRENALINA-INDUZIDAS
JAIRO AUGUSTO BERTI1; MARIA M.D. PEDROSA1; DIEGO G.L. REZENDE1; MARCOS A. PRIMO1; HELENA
C.F. OLIVEIRA2; VILMA A.F. GODOI1
1.UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL; 2.UNIVERSIDADE ESTADUAL DE
CAMPINAS, CAMPINAS, SP, BRASIL.
Camundongos C57BL6 transgênico para apo-CIII humana apresentam alterações do metabolismo
lipídico e glicêmico. Sendo assim, estudamos a glicogenólise e glicólise hepáticas através da
técnica de perfusão de fígado in situ. Vinte camundongos machos (120d, 28g) alimentados foram
utilizados. Os camundongos transgênicos (grupo CIII) apresentaram trigliceridemia>300mg/dL e os
controles não transgênicos (grupo NTG) trigliceridemia<100mg/dL. Os fígados foram perfundidos
com tampão por 30min (basal) e com tampão+adrenalina (1µM) por 30min (estimulado). Glicose,
lactato e piruvato foram dosados (µmol/min.g fígado) e a área sob a curva (µmol/g fígado)
calculada. CIB (3615/2013), CEUA (020/2013). Dados: média±dp, n=5/grupo; teste t, p<0,05. Na
presença da adrenalina, o grupo CIII liberou mais piruvato (NTG=0,655±0,178, CIII=1,049±0,167;
p<0,05), menos lactato (NTG=18,650±5,554, CIII=7,718±1,395; p<0,05) e glicose
(NTG=68,235±7,432, CIII=25,955±3,959; p<0,05). Apresentou taxa reduzida de glicogenólise
(NTG=42,778±2,231, CIII=16,251±1,275, p<0,05) e de glicólise (NTG=19,174±2,324,
CIII=6,547±1,554, p<0,05), e reduzida razão NADH/NAD+citosólica (NTG=18,650/0,655=28,47,
CIII=7,720/1,049=7,36). Camundongos C57BL6 apoCIII apresentaram: taxa glicolítica reduzida,
efeito provável da inibição dasfosfofrutoquinase e piruvatodesidrogenase; glicogenólise e
liberação hepática glicose reduzidas, indicando um possível efeito na atividade da glicogênio
sintase pelos produtos da beta oxidação.
09.005 - THE EXPRESSION OF CIRCADIAN CLOCK IN ANTERIOR PITUITARY GLAND IS ALTERED
DURING THE ADULTHOOD HYPOTHYROIDISM
PAULA BARGI-SOUZA; RODRIGO ANTONIO PELICIARI-GARCIA; RAFAEL MASO PRÉVIDE; MARIA TEREZA
NUNES
INSTITUTE OF BIOMEDICAL SCIENCES OF UNIVERSITY OF SÃO PAULO, SP, BRAZIL, SAO PAULO, SP,
BRASIL.
The anterior pituitary gland occupies a central position in the hypothalamus-pituitary-glands axes
and the plasma concentrations of its hormones present fluctuations during the 24 h. The presence
of an intra-pituitary circadian oscillator might be related to these oscillations; however, the
consequences of the hypothyroidism are still unknown. The purpose of the present study was to
investigate the expression of Bmal1, Per2, Dbp, Nr1d1, Rora and Dio2 during the adult
hypothyroidism. For this, euthyroid (C) and thyroidectomized (Tx) male Wistar rats were
euthanized during 24 h, every 6 h. The pituitaries were excised and the mRNA expression was
evaluated by RT-qPCR. One and Two-Way ANOVA, as well as, cosinor analysis were used to
evaluate the time-of-day-dependent differential expression for each gene/group and their
interactions. The expression of Bmal1, Per2, Dbp, Nr1d1, Rora and Dio2 presented a circadian
pattern in anterior pituitary of C rats and the peak of Per2, Dpb, Rora and Dio2 expression occurred
at ZT 12, while for Bmal1 was ZT 0/24. In the hypothyroid animals, the circadian pattern of Bmal1,
Rora and Dio2 was lost and the acrophase of Per2, Dbp and Nr1d1 was advanced about 2.5 h, 3 h
and 45 min, respectively. Tx also reduced Mesor values of Dbp and Nr1d1. Our studies reveal that
the expression of core clock and clock-controlled genes in anterior pituitary gland are changed
during the hypothyroidism and might contribute to the altered hormonal pattern of secretion
observed in this pathological condition. Further studies are in progress to assess this issue.
09.006 - GLICOGENÓLISE HEPÁTICA ESTIMULADA POR ADRENALINA PODE PARTICIPAR DO
MELHOR PERFIL HIPOGLICÊMICO EM RATOS SOB RESTRIÇÃO ALIMENTAR DESDE O NASCIMENTO
ANA MARIA PRAXEDES DE MORAES; CAMILA CRISTINA IANONE MATIUSSO; VILMA A.F. GODOI; MARIA
M.D. PEDROSA
UNIVERSIDADE ESTADUAL DE MARINGÁ, APUCARANA, PR, BRASIL.
Uma sessão de exercício físico incremental antes de um episódio de hipoglicemia induzida por
insulina (HII) eleva a glicemia basal, reduz a severidade do perfil hipoglicêmico e melhora a
recuperação da glicemia em ratos sob restrição alimentar prolongada. Avaliou-se se a liberação
hepática de glicose estimulada por adrenalina (gli-ADR) poderia colaborar para esse quadro. Ratos
Wistar machos do grupo controle (GC, ninhada de 6 filhotes, alimentação livre) e do grupo
restrição (GR, ninhada de 12 filhotes, redução de 50% da alimentação), aos 60 dias de idade, após
jejum noturno, foram submetidos à HII (1 U/kg) seguida de perfusão hepática in situ com ADR
(1μM), 60 min e 300 min após a administração de insulina. Os dados são mostrados como média±DP,
n=5-8/grupo. Aos 60 min, a gli-ADR foi maior no GR do que no GC (GC = 2,33±0,65, GR = 3,10±1,47
µmol.g-1, p<0,05, teste t). Aos 300 min, essa diferença foi ainda mais significativa (GC =
1,66±0,46, GR = 2,88±0,72 µmol.g-1, p<0,01, teste t). As taxas de glicogenólise também foram
mais altas no GR do que no GC em ambos os tempos: GC = 2,66, GR = 3,80 µmol.g-1 aos 60 min;
GC = 2,02, GR = 3,57 µmol.g-1 aos 300 min). Estes dados sustentam a hipótese de que a ADR
liberada pelo exercício físico agudo, por meio da estimulação da glicogenólise hepática, pode ter
contribuído para o perfil hipoglicêmico observado in vivo nos ratos sob restrição alimentar
prolongada.
09.007 - GLUTAMINA E GLUTAMINA DIPEPTÍDEO REDUZEM A GLICONEOGÊNESE EM RATOS COM
DIABETES MELLITUS TIPO 1 DESCOMPENSADO
SILVIA CARLA SANTANA AZEVEDO; MARIA M.D. PEDROSA; CARLOS VINICIUS DALTO DA ROSA; CAMILA
BATAGLINI; DIEGO G.L. REZENDE; MARCOS A. PRIMO; ROBERTO BARBOSA BAZOTTE; VILMA A.F.
GODOI
A glutamina está reduzida no diabetes mellitus tipo 1 (DM1), mas os benefícios da sua
suplementação não estão claros. Por isso, avaliou-se a gliconeogênese (GNG) em hepatócitos
isolados de ratos Wistar machos DM1 (STZ, 60 mg/Kg, iv) suplementados com glutamina (GLN) ou
glutamina dipeptídeo (GDP). Os animais diabéticos formaram os grupos: D (salina oral); DGLN (248
mg/Kg GLN oral); e DGDP (400 mg/Kg GDP oral), tratados por 30 dias. Dados: média±DP, em
μmol.106 cels.hr, n= 4/grupo, teste t, p<0,05. Em todos os grupos, a GNG na presença de
precursores foi maior do que no estado basal, que foi: D=0,19±0,01; DGLN=0,14±0,03;
DGDP=0,10±0,02. A GNG foi mais elevada a partir de lactato nos grupos D (0,89± 0,04) e DGDP
(0,60± 0,02) e no DGLN a partir de glicerol (0,46±0,04). A GNG a partir de glicerol aumentou no
grupo DGLN e diminuiu no DGDP em relação ao grupo D (D=0,36±0,03; DGLN=0,46±0,04;
DGDP=0,26±0,02). A GNG a partir de lactato foi menor nos grupos DGLN (0,27±0,03) e DGDP
(0,60±0,02) do que no grupo D (0,90±0,04), assim como a partir de glutamina (D=0,32±0,03;
DGLN=0,22±0,04; DGDP=0,24±0,03). A GNG a partir de alanina não foi alterada (D=0,36±0,06;
DGLN=0,36±0,07; DGDP=0,34±0,04). A gliconeogênese reduzida nos grupos DGLN e DGDP em
relação ao grupo D poderia resultar em menor acúmulo de glicogênio pela via indireta e redução
da glicogenose secundária, decorrentes do DM1 descompensado, o que aponta para um efeito
benéfico da GLN e do GDP nesse quadro.
09.008 - RESTRIÇÃO CALÓRICA MODERADA REDUZ A ADIPOSIDADE E A GLICEMIA DE JEJUM DE
RATOS DE NINHADAS REDUZIDAS
NAYRA DELATORRE BRANQUINHO; VILMA A.F. GODOI; MARIA M.D. PEDROSA; LETICIA DINIZ
CREPALDI; GABRIEL HENRIQUE DE PAULA CRUZ; CAMILA BATAGLINI
UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL; ; ; .
A restrição calórica moderada é uma profilaxia contra os distúrbios metabólicos (Dmt) e
cardiovasculares (Dcv) da obesidade. Foi registrado o padrão biométrico de ratos de ninhadas de
3 filhotes, um modelo de obesidade (NR), com alimento livre (NRL) ou sob restrição calórica de
30% (NRR), e de animais de ninhadas de 9 filhotes (controles, NC). Os ratos foram eutanasiados
aos 21 e 90 dias de idade, após jejum noturno (14 hr). Dados mostrados como média±DP, n=4-11,
analisados por teste t, p<0,05. Aos 21 dias, o NR teve valores maiores do que o NC quanto a peso
corporal (47,1±2,3 vs. 39,5±3,1 g), gordura subcutânea (0,13±0,04 vs. 0,09±0,01 g/10g) e visceral
(0,13±0,02 vs. 0,08±0,01 g/10g) e glicemia (123,4±22,6 vs. 102,3±13,5 mg/dL). Aos 90 dias, o peso
corporal não diferiu entre o NRL e o NC, mas foi menor no NRR (NC=347,3±23,7; NRL=354,6±36,4;
NRR=136,5±4,8 g). O mesmo foi observado para a gordura subcutânea (NC=1,22±0,22;
NRL=1,32±0,28; NRR=0,90±0,20 g/100g) e a glicemia (NC=76,18±7,95; NRL=73,71±6,53;
NRR=43,50±6,61 mg/dL). A gordura visceral foi maior no NRL do que no NC, e menor no NRR do
que nos outros grupos (NC=2,78±0,45; NRL=3,58±0,97; NRR=1,19±0,16 g/100g.). A redução da
ninhada (NR) e o alimento livre (NRL) aumentaram a gordura visceral, a mais prejudicial para Dmt
e Dcv. A restrição calórica de 30% (NRR) reduziu acentuadamente o peso corporal e a adiposidade,
mas a baixa glicemia de jejum é relevante pela importância desse parâmetro para a homeostase
fisiológica.
09.009 - ASSESSMENT OF THE AKT PHOSPHORYLATION STATUS AND THE GLUT4 EXPRESSION IN
MUSCLE TISSUE OF RATS TREATED WITH NAF
FERNANDO YAMAMOTO CHIBA; MARIANA LOPES BENITES; RENATO FELIPE PEREIRA; THAÍS SAORI
TSOSURA; NATALIA FRANCISCO SCARAMELE; AMANDA GOMES PEREIRA; MURILO CARETA
GUIMARÃES; RENAN AKIRA OLIVEIRA; DORIS HISSAKO SUMIDA
UNIV ESTADUAL PAULISTA - UNESP, ARACATUBA, SP, BRASIL.
Over the last years, there has been a significant reduction in the incidence of dental caries in
several regions of the world. This has been attributed to the use of fluoridated products, such as
toothpaste. However, excessive NaF intake causes glycolysis inhibition, decrease on insulin
secretion and hyperglycemia, suggesting that NaF may cause insulin resistance. Therefore,
became fundamental to examine the chronic effect of NaF on: 1) Akt phosphorylation status; 2)
GLUT4 expression in skeletal muscle gastrocnemius (SMG). For this study, castrated Wistar male
rats (1 month of age) were used. Thirty days after castration, the animals were be randomly
divided in two groups: 1) control group (CN); 2) group NaF (FN) that was submitted to treatment
with NaF administered in the drinking water and F contained in food pellets (F total inferred: 4.0
mg F / Kg bw / day in the form of NaF) during 42 days. After 6 weeks, the quantification of the
Akt phosphorylation status, after insulin stimulation, in muscle, liver and white adipose tissue by
western blotting method and the evaluation of GLUT4 expression in SMG by RT-PCR method were
performed. Chronic treatment with NaF promoted: 1) a decrease in the Akt phosphorylation status
in the muscle and in the white adipose tissue (p <0.05); 2) no change in the Akt phosphorylation
staus in the liver; 3) reduction of GLUT4 expression in the SMG.
09.010 - CALORIC RESTRICTION (CR) INCREASES INSULIN TOLERANCE BOTH IN
HYSTERECTOMIZED OVARIAN SALPINGO RATS (OSH) AND IN SHAM RATS
DANIELLA ROSALY LEITE; JÉSSICA TONETTI CUNHA; ÉRIKA CRISTINA VENTURA; THAÍS FURTADO
CAMARGO; SABRINA MARTIN FELIPE; MATHEUS EDUARDO BORTOLANSA DA SILVA; MARIA ESMERIA
COREZOLA AMARAL
Absence of female hormones promotes metabolic changes as hyperphagia and the consequent
weight gain associated to insulin resistance by peripheral tissues and diabetes type 2.
The aim of this study was to investigate the effects of CR in such metabolic disorders. It was
performed in female Wistar rats divided into groups: ShamC (diet ad libitum) ShamR (40% CR),
OSHC (OSH + C) and OVHR (OSH + R). After eight weeks of surgery the rats were submitted to the
CR for 28 days.
It was analyzed glycemic and lipid profile, ip.GTT (the intraperitoneal glucose tolerance test),
the ip.ITT (intraperitoneal insulin tolerance test) liver and muscle glycogen.
Blood glucose of ShamR and OSHR rats was less than ShamC and OSHC. There was a reduction in
the perirenal adipose tissue of ShamR and OSHR rats compared to ShamC and OSHC. Data
characterize the effects of CR. Cholesterol, triglyceride, ip.GTT and muscle glycogen were similar
for all groups.
OSHR had higher levels of liver glycogen compared to ShamC, ShamR and OSHC and suggests
decreased mobilization of the substrate and consequent reduction of circulating glucose. The Kitt
(constant for glucose decay calculated from ip.ITT) was higher for ShamR and OSHR than to ShamC
and OSHC and suggests suppression of gluconeogenesis and increased glucose uptake by insulinsensitive tissues. Data indicate that the rats submitted to RC, ShamR and OSHR, showed higher
tolerance to insulin with changes of hepatic glycogen in OSHR group.
09.011 - NEONATAL OVERFEEDING IMPAIRS HORMONAL CONTROL OF APPETITE AND BODY
WEIGHT GAIN IN ADULTHOOD
SILVIA ENES MARQUES; ALEXANDRE GIUSTI PAIVA
FEDERAL UNIVERSITY OF ALFENAS, ALFENAS, MG, BRASIL.
AIM:evaluate metabolic parameters, glucose-stimulated insulin secretion e anorexigenic effect of
peripheral glucagon-like pepitide-1 (GLP-1) in neonatally overfed rats. METHODOLOGY:At
postnatal day 1 (PND1), the litters (n=8) were adjusted to small litter (SL, n=3/litter) or normal
14
litter (NL, n=12/litter). The body weight, plasma levels of glucose, triglycerides, cholesterol and
its fractions, insulin and leptin, glucose (GTT) and insulin (ITT) tolerance tests and studies food
intake were performed in males at PND 60. The procedures were approved by the CEUA UNIFAL
(568/2014). RESULTS:The body weight was higher in the SL group since PND8 until adulthood
compared to NL group (p<0.001). The males SL group presented increased plasma level of glucose
(105±0.9 to 118±2.7mg/dL; p<0.001), triglycerides (135±11.7 to 169±10.6mg/dL, p<0.05), total
cholesterol (70±3.7 to 85±4.1mg/dL, p<0.05), plasma leptin (0.68±0.1 to 1.92±0.2, p<0.01)
compared with NL group. Males SL group exhibited decreased glucose tolerance (p<0.05) and
reduced insulin sensitivity (p<0.001). GLP-1 (10 μg/kg) significantly reduced 30min (-57%,
p>0.001), 60min (-66%, p>0.001), 90min (-60%, p>0.001), 120min (-45%, p>0.01) and 24h (-12%,
p>0.05) food intake in males NL but did not alter the food intake SL rats. CONCLUSION: The
neonatal overfeeding can disrupt the hormonal control of weight gain, of glucose homeostasis and
ability of peripheral GLP-1 to inhibit food intake of adult rats.
09.012 - ACTIVITIES OF NO- AND H2S-GENERATING HYPOTHALAMIC ENZYMES ARE BOTH
INCREASED IN VITRO BY EXTRACELLULAR HYPEROSMOLALITY
RICARDO COLETTI1; FERNANDA MARIA VEANHOLI VECHIATO1; JULIANA BEZERRA MEDEIROS DE
LIMA1; GISLAINE ALMEIDA-PEREIRA1; FABIANA LUCIO-OLIVEIRA2; LUCILA LEICO KAGOHARA ELIAS1;
JOSÉ ANTUNES-RODRIGUES1
1.FMRP/USP, RIBEIRÃO PRETO, SP, BRASIL; 2.IFSULDEMINAS, MUZAMBINHO, MG, BRASIL.
NO inhibits hypothalamic neurons depolarization which decreases vasopressin (AVP) and oxytocin
(OT) release, while H2S induces depolarization that increases hormones release (HR); however, no
experimental model of easy execution and that allows to assess NO and H 2S generation and
interaction in the medial basal hypothalamus (MBH) has been described.
Thus we evaluated if the MBH incubation in vitro led to responses of HR and enzymatic activity
(EA) according to the observed in vivo. To that, male Wistar rats’ (270-300g) MBH were collected
and kept in isotonic (280 mOsm/Kg H2O) oxygenated Krebs-Ringer bicarbonate buffer (KRBG) for
preincubation (37°C, 1L/min carbogenic mixture, 60 min). Then, medium was replaced by new
hypertonic (340 mOsm/Kg H2O) or isotonic KRBG (incubation: 37°C, 1L/min carbogenic mixture,
30 min) from which samples were collected for AVP, OT and ANP radioimmunoassay. Afterwards,
MBH were submitted to specific EA assays in which NO synthase (NOS) activity was measured by
14
C-citrulline generation and H2S-generating enzymes activity (CBS/3MST) was assessed by sulfides
formation.
MBH exposure to hypertonic KRBG increased AVP (t31 = 7.6, p < .0001), OT (t29 = 7.2, p < .0001)
and ANP (t37 = 6.9, p < .0001) release, along with NOS (t12 = 2.2, p < .05) and CBS/3MST (t9 = 3.3,
p < .01) activity, demonstrating that MBH incubation leads to response patterns congruent to that
observed in vivo in relation to HR and NO and H2S formation in response to extracellular
hypertonicity.
09.013 - ANTI-DIABETIC EFFECTS OF ETHYL-ACETATE FRACTION FROM TRICHILIA CATIGUA IN
STREPTOZOTOCIN-INDUCED TYPE 1 DIABETIC RATS
RODRIGO MELLO GOMES; FLÁVIO ANDRADE FRANCISCO; CAMILA QUAGLIO NEVES; BRUNA COLOMBO
CORDEIRO; VILMA A.F. GODOI; PAULO CESAR DE FREITAS MATHIAS; RENATA LONGHINI; JOÃO
CARLOS PALAZZO DE MELLO; MARIA RAQUEL MARÇAL NATALI
UEM, MARINGA, PR, BRASIL.
Background: In Brazil Trichilia catigua A. Juss. is known as “catuaba” and have been popularly
used as a tonic for the treatment of fatigue, stress, impotence and deficit memory. The present
study evaluated the anti-diabetic activity of the ethyl-acetate fraction (EAF) of Trichilia catigua.
Methods: Male Wistar rats were divided into four groups N: normal glycemic, D: diabetics, NC:
normal glycemic + EAF (200 mg/kg) and DC: diabetic + EAF (200 mg/kg). Diabetes was induced by
a single STZ (35 mg/kg) injection into the penile vein. Two days after STZ injection the EAF was
orally administered 7 days per week, during 58 days, by gastric gavage. Results: Rats from D group
showed reduced body weight, adipose tissue and muscular mass (p<0.05). This animals presented
increased food and water intake and hyperglycemia with atrophy of pancreatic islets by β-cell
death (p<0.05). However, EAF administration in DC group improves overall body mass loss and
reduced food and water intake (p<0.05). In addition, EAF treatment in DC rats promoted an antidiabetic effect with 24% reduction in blood glucose (p<0.05). Furthermore, anti-insulin
immunohistochemistry analysis shows significant increased β-cell number and islets size in DC
group by β-cell proliferation labeled with PCNA immunohistochemistry. Conclusions: The present
results suggest that EAF of T. catigua ameliorates glucose homeostasis and endocrine pancreas
injury caused by STZ in diabetes type 1 rat model.
09.014 - EFEITOS DE ESTEROIDES OVARIANOS, SORO FETAL BOVINO E TEMPO DE CULTURA
SOBRE A VIABILIDADE DE OSTEOBLASTOS IN VITRO
LUCAS FC BRANCAGLIÃO; VÍTOR M GONÇALVES; JOSÉ ES LEMOS; NÍCOLAS F ROCHA; RENATA
AQUINO; ANDRÉ C AMARAL; LUIS HENRIQUE MONTREZOR
CENTRO UNIVERSITÁRIO DE ARARAQUARA - UNIARA, ARARAQUARA, SP, BRASIL.
O presente trabalho estudou os efeitos de diferentes concentrações (0, 10-8 M e 10-7 M) de
progesterona (P4), testosterona (T) e estradiol (E2); do tempo de cultura (48 e 72 horas) e da
ausência e presença de soro fetal bovino (SFB) sobre a viabilidade de osteoblastos in vitro. As
células foram cultivadas em placas de 96 poços com meio DMEM suplementado com 100 U/mL de
penicilina e 100 𝜇g/mL de estreptomicina na densidade de 5 x 103 células/100 𝜇L de meio, sem
ou com 10% de SFB. As células foram mantidas em estufa a 37 ºC, 5% de CO2 e 95% de ar. A
viabilidade celular foi analisada pelo ensaio colorimétrico do MTT. A viabilidade foi maior para as
células cultivadas na presença de SFB em ambos os tempos e para todos os hormônios testados,
comparado às culturas sem o SFB. E2, P4 e T estimularam a viabilidade das células cultivadas por
48 h na ausência de SFB; com 72 h de cultura, E2 e P4 estimularam a viabilidade celular, a T
induziu efeito dual e todos os hormônios juntos estimularam a viabilidade. Na presença de 10% de
SFB, com 48 h de cultura, a dose de 10-7 M de E2 estimulou a viabilidade celular e com todos os
hormônios juntos, na dose de 10-7 M, houve redução da viabilidade. Com 72 h de cultura, a T e
todos os hormônios juntos, na dose de 10-8 M, diminuíram a viabilidade das células. Os resultados
sugerem que os esteroides ovarianos modulam a viabilidade dos osteoblastos, de maneira dosedependente, na ausência e na presença de SFB em 48 h e 72 h de cultura.
09.015 - EVALUATION OF INSULIN SENSITIVITY AND AKT PHOSPHORYLATION STATUS IN MUSCLE
TISSUE OF OVARIECTOMIZED RATS TREATED WITH SODIUM FLUORIDE
AMANDA GOMES PEREIRA; RENATO FELIPE PEREIRA; FERNANDO YAMAMOTO CHIBA; THAÍS VERÔNICA
SAORI TSOSURA; MARIA SARAA DE LIMA COUTINHO MATTER; DORIS HISSAKO SUMIDA
UNIVERSIDADE ESTADUAL PAULISTA, ARAÇATUBA, SP, BRASIL.
Sodium fluoride (NaF) has been studied as a possible therapeutic agent for osteoporosis in
postmenopausal women. However, high doses of NaF can cause hyperglycemia, inhibition of
glycolysis and decreased insulin secretion. The Akt protein participates actively in insulin signaling
pathways by mediating the metabolic effects of this hormone in different tissues. The aim of this
study was to evaluate the Akt serine phosphorylation status in the gastrocnemius muscle tissue
(GM) and insulin resistance through the HOMA-IR index in ovariectomized rats chronically treated
with NaF. Fourty Wistar rats were ovariectomized and randomly distributed in two groups: 1)
control group (OVX-C); 2) group submitted to treatment with NaF (OVX-F) at a dose of 50 mg/L in
drinking water for 42 days. Statistical analysis was performed by analysis of variance (ANOVA)
followed by Tukey test. The significance level adopted was 5%. The treatment with NaF promoted:
1) decreased (p <0.05) Akt serine phosphorylation staus, after insulin stimulation, in GM; 2)
increase (p <0.05) in the HOMA-IR index compared to the OVX-C group. It is concluded that chronic
treatment with NaF (50 mg/L) induced insulin resistance and decreased insulin signaling in MG
rats. Therefore, we suggest caution in the use of NaF for the treatment of osteoporosis in
postmenopausal women
09.016 - PERIAPICAL LESION DECREASES GLUT4 TRANSLOCATION INDEX AND INCREASES IKK
AND JNK PHOSPHORYLATION IN SKELETAL MUSCLE TISSUE IN RATS
RENATO FELIPE PEREIRA; MARIANA LOPES BENITES; THAÍS SAORI TSOSURA; NATALIA FRANCISCO
SCARAMELE; FERNANDO YAMAMOTO CHIBA; AMANDA GOMES PEREIRA; DORIS HISSAKO SUMIDA
UNESP, ARAÇATUBA, SP, BRASIL.
Previous studies from our laboratory demonstrated that adult rats with periapical lesion (PL)
showed changes insulin signaling and impaired insulin sensitivity. However, the mechanisms
responsible for these changes are unclear. The kinases IKK (IkB kinase) and JNK-1 (c-Jun Nterminal kinase 1) negatively regulate the insulin signaling. This study aimed to evaluate the
GLUT4 translocation index to the plasma membrane, and the IKK and JNK phosphorylation in
skeletal muscle of rats with PL. Wistar rats were divided into two groups: control (CN) and
periapical lesion (PL). The PL was induced in the upper right molars employing carbon steel drill
with sphere on the edge with 0.1 mm. The GLUT4 translocation index was calculated from the
formula: GLUT4 translocation index (%) = plasma membrane GLUT4 x 100 / (plasma membrane
GLUT4 + microsomal GLUT4). The IKK and JNK phosphorylation status were evaluated by Western
blotting method. Statistical analysis was performed by Student's t test. The PL group showed a
reduction of the GLUT4 translocation index to plasma membrane content and increased IKK and
JNK phosphorylation in skeletal muscle. These results indicate that oral inflammation can cause
inflammation in skeletal muscle which that impairs insulin signaling in this tissue.
09.017 - SOLIDAGO CHILENSIS AND LASER ENHANCES THE REPAIR OF BURNS IN DIABETIC RATS
VIVIANE THEODORO; JULIANA APARECIDA RAMIRO MOREIRA; ISRAEL COSTA VASCONCELOS; JOSÉ
LUIS FACHI; FERNANDA OLIVEIRA GASPARI GASPI; RODRIGO AUGUSTO DALIA; MARCELO AUGUSTO
MARRETO ESQUISATTO; FERNANDA APARECIDA SAMPAIO MENDONÇA; GLÁUCIA MARIA TECH
SANTOS; MARIA ESMERIA COREZOLA AMARAL
CENTRO UNIVERSITÁRIO HERMINIO OMETTO, ARARAS, SP, BRASIL; ; ; ; ; ; ; ; .
Medicinal plants and low-intensity laser present a high potential in the treatment of wounds and
burns. The objective of this study was to investigate the effect of the hydroalcoholic extract of
the leaves of Solidago chilensis Meyen associated with the application of InGaP-670nm laser in
repair of second-degree burns, were made on the back of diabetic Wistar rats. Thirty-two alloxan
diabetic rats, characterized with glucose above 250 mg/dL, were anesthetized and the burn was
performed with aluminum plate (2cm diameter/120°C/20s). The animals were divided into four
groups: (C) no treatment; (S) treated with extract of S. chilensis; (G) treated with laser 670-nm
InGaP; (LS) treated with laser associated with the S. chilensis extract. Samples were collected
from lesions on the 7, 14 and 21 days for protein expression by Western blotting of TGF-β1, VEGF,
bFGF, Collagen I and III. On the 14 day was observed a significant increase in VEGF expression in
S and L. Both TGF-β1 expressions as bFGF were not significant during the experimental period in
all groups. With respect to deposition of Collagen I was observed on the 14 day significant increase
in L and LS groups and, on the 21 day, the S and L groups. The expression of Collagen III
significantly decreased after 14 day in L and LS groups and, after 21 day, in the treated groups.
S. chilensis and laser 670-nm InGaP promoted angiogenesis and collagen reorganization in repair
burns in diabetic rats.
09.018 - EFFECT OF CHRONIC STRESS ASSOCIATED TO HYPERCALORIC DIET CONSUMPTION ON
THE ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME AND RENIN, IN RATS
FERNANDA KLEIN MARCONDES1; RAFAELA COSTA1; ANDREA SANCHES1; FERNANDA RONCHI2; ZAIRA
PALOMINO JARA2; DULCE ELENA CASARINI2
1.FACULDADE DE ODONTOLOGIA DE PIRACICABA - UNIVERSIDADE ESTADUAL DE CAMPINAS,
PIRACICABA, SP, BRASIL; 2.FEDERAL UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL.
Previously we´ve observed that chronic mild and unpredictable stress (CMUS) induced
dyslipidemia, endothelium dysfunction in the thoracic aorta, increased the activity of circulating
and aortic renin angiotensin system in rats, and losartan cancelled vascular effect of CMUS.
Stressed rats treated with hypercaloric diet (HD) also presented dyslipidemia and similar vascular
effects. However non stressed rats treated with HD only presented dyslipidemia without vascular
changes. In order to evaluate the relation among RAS activity, HD and CMUS effects, the aim of
the present work was to study the effects of CMUS and HD on the activity of circulating and aortic
angiotensin converting enzyme and plasma renin activity. Male rats were randomly assigned to
four groups: Control, HD, CMUS, and HD-CMUS. CMS consisted of the application of different
stressors for 3 weeks. The rats were killed 15 days after CMUS exposure. CMUS group presented
higher activity of renin in plasma, and higher angiotensin converting enzyme activity in the
thoracic aorta, in comparison with control, HD and HD-CMUS groups (p < 0.05), without difference
among control, HD and HD-CMUS groups. CMUS, HD and HD-CMUS groups presented higher activity
of seric angiotensin converting enzyme in comparison with control group. These results and the
previous data indicate that vascular effects of CMUS observed in rats treated with HD, seem not
to be related to changes in the activity of renin and angiotensin converting enzyme induced by
CMUS.
09.019 - EXERCÍCIO AGUDO INCREMENTAL MELHORA PERFIL HIPOGLICÊMICO EM RATOS SOB
RESTRIÇÃO ALIMENTAR DESDE O NASCIMENTO – POSSÍVEL EFEITO DA ADRENALINA SOBRE A
GLICOGENÓLISE HEPÁTICA
CAMILA IANONI MATIUSSO
Uma sessão de exercício físico incremental melhora os episódios de hipoglicemia induzida por
insulina (HII) em ratos sob restrição alimentar prolongada. Por isso, investigou-se a liberação
hepática de glicose estimulada por adrenalina (gli-ADR) em animais submetidos a exercício agudo.
Ratos Wistar dos grupos controle (GC, ninhada de 6 filhotes, alimentação livre) e restrição (GR,
ninhada de 12 filhotes, redução de 50% da alimentação), aos 60 dias de idade, após jejum noturno,
foram submetidos a HII (1 U/kg) e perfusão hepática in situ com ADR (1μM), ambos com sessão
prévia de exercício físico incremental em esteira. Os dados são mostrados como média±DP, n=58/grupo. A sessão de exercício antes da HII elevou a glicemia basal (GC=67,08±5,12,
GR=92,21±8,09 mg.dL-1, p<0,01, teste t), reduziu a severidade do perfil hipoglicêmico e melhorou
a recuperação da glicemia do GR (GC=39,12±9,6, GR=69,71±8,93 mg.dL-1 aos 300 min, p<0,01,
teste t). A gli-ADR foi maior no GR do que no GC após o exercício incremental (GC=1,46±0,21,
GR=2,67 µmol.g-1, p<0,01, teste t). A gli-ADR se mostrou preservada nos animais sob restrição
alimentar desde o nascimento, mesmo após jejum noturno, o que justifica, ao menos
parcialmente, a glicemia pós-exercício mais elevada, a hipoglicemia menos severa, e a melhor
recuperação da glicemia durante o episódio de HII e após sessão aguda de exercício observada in
vivo.
09.020 - RESPOSTAS VENTILATÓRIAS E TÉRMICAS À HIPÓXIA E HIPERCAPNIA EM RATAS COM
SÍNDROME DO OVÁRIO POLICÍSTICO
LUIS HENRIQUE MONTREZOR1; DÉBORA CARVALHO2; JANETE APARECIDA ANSELMO-FRANCI3; KÊNIA
CARDOSO BÍCEGO2; LUCIANE HELENA GARGAGLIONI BATALHAO 2
1.CENTRO UNIVERSITÁRIO DE ARARAQUARA - UNIARA, ARARAQUARA, SP, BRASIL; 2.FACULDADE
DE CIÊNCIAS AGRÁRIAS E VETERINÁRIAS - UNESP, JABOTICABAL, SP, BRASIL; 3.FACULDADE DE
ODONTOLOGIA DE RIBEIRÃO PRETO - USP, RIBEIRÃO PRETO, SP, BRASIL.
Foram analisadas a ventilação pulmonar e a temperatura corporal durante hipercapnia (7% de CO2)
e hipóxia (7% de O2) em ratas induzidas à síndrome do ovário policístico (SOP) com injeção de
valerato de estradiol (VE) (2 mg/0,2 mL/rato) ou óleo (controle); 30, 45 ou 60 dias após a injeção
de VE. Os ovários das fêmeas induzidas à SOP demonstraram alterações morfológicas típicas 30,
45 e 60 dias após a injeção de VE. As concentrações plasmáticas de estradiol (E2) e progesterona
(P4) aumentaram nos grupos 30 (E2: 74,5 ± 23,5 pg/mL; P4: 18,3 ± 4,5 ng/mL), 45 (E2: 70,8 ± 17,5
pg/mL; P4: 21,3 ± 7,6 ng/mL) e 60 (E2: 71,4 ± 4,7 pg/mL; P4: 23,7 ± grupos 4,5 ng/mL) dias de
VE enquanto que a testosterona (T) aumentou nos grupos 45 (58,4 ± 6,5 pg/mL) e 60 (79,5 ±
grupos/mL) dias de VE em comparação com o grupo controle (E2: 46,9 ± 11,3 pg/mL; P4: 9,9 ±
3,4 ng/mL; T:. 22,6 ± 1,9 pg/mL). O tratamento com VE não afetou a ventilação em repouso nem
a resposta ventilatória à hipóxia. No entanto, o aumento na ventilação induzida pela hipercapnia
foi atenuada no grupo VE 30 dias (1978,9 ± 85,7 mL.kg-1.min-1 grupo controle; 1651,2 ± 369,3 grupo
45 dias e 1617,4 ± 131,4 grupo 60 dias vs 771,0 ± 172,4 grupo 30 dias). Houve redução da Tc
induzida pela hipóxia em todos os grupos. Nossos resultados sugerem que as alterações iniciais
promovidas pelo tratamento com VE atenua temporariamente o quimiorreflexo ao CO 2, e estas
respostas são compensadas em 45 e 60 dias após o tratamento.
THEME 10 – PHYSIOLOGY EDUCATION
15
10.001 - POPNEURO: POPULARIZING NEUROSCIENCE AND AWAKENING THE CURIOSITY OF
SCHOLARS ABOUT THE NERVOUS SYSTEM
GEORGIA ELISA FILIPIN; PAMELA BILLIG MELLO CARPES; BRUNA MEYER PERRONI
UNIPAMPA, URUGUAIANA, RS, BRASIL.
It is known that neuroscience has gained great prominence by his findings, especially when related
to education. Considering this, our aim is to report a program of neuroscience popularization in
public schools (POPNEURO). The activities were composed by weekly visits including a brief
theoretical explanation and practical activity addressing neuroscience topics, held in four public
schools of Uruguaiana/RS/Brazil. The proposed themes were: (i) What is science and
neuroscience; (ii) The brain (neuroanatomy); (iii) How neurons talk to each other (synapse); and,
(iv) Sensory perception. To evaluate the actions pre and post-intervention questionnaires were
applied. 113 students from 9 to 13 years-old participated in the actions. At the beginning of the
action (i) 63% of students said that they knew what a scientist makes; the percentage increased
to 88% after the action. In action (ii) we find that only 4% of students knew what neuroanatomy
is; at the end, 52% knew. In action (iii) the concept of synapse, were initially known by 4% of the
students, but in the end by 84% of them. Prior to action (iv) only 10% of students know what is
sensory perception, percentage reached 75% after the intervention. In general, the activities were
well accepted, and students gave a grade of 9.6 (considering a scale of 0 to 10). Thus we see that
the actions accomplished their goal of bringing neuroscience to the school, popularizing important
concepts to the daily lives of students.
10.002 - CAUSA E EFEITO NAS RESPOSTAS DE REGULAÇÃO DA PRESSÃO ARTERIAL: APLICAÇÃO
DE UM JOGO DE PEÇAS
JULIANA IRANI FRATUCCI DE GOBBI; SILVIA M NISHIDA
DEPT PHYSIOLOGY, INSTITUTE OF BIOSCIENCE, UNESP, BOTUCATU, BOTUCATU, SP, BRASIL.
A regulação da pressão arterial (PA) é um tema considerado complexo para os alunos de
graduação. Geralmente, os alunos confundem a relação causa-e-efeito neste processo. Com a
finalidade de incentivar a compreensão dos mecanismos de regulação da PA elaboramos um jogo
onde os alunos escolhem, dentre várias possibilidades, o que representa a causa e a resposta dos
mecanismos de controle da PA tendo como cenário da problematização, situações do cotidiano. A
aplicação desta atividade ocorreu após aulas expositivas e estudos dirigidos sobre os conteúdos
básicos. A turma foi dividida em 4 grupos, contando com 20 minutos para a resolução do problema.
Cada grupo recebeu um caso e um conjunto de 20 figuras (extraídas do Google Image), palavraschave e setas indicando aumento e diminuição de uma determinada variável. As figuras foram
escolhidas para preencherem um pôster onde deveria constar do lado esquerdo a causa e do lado
direito a resposta para o caso em questão. Cada grupo compartilhou a resolução do seu caso para
a turma. A atividade foi corrigida em conjunto para que toda a turma tivesse acesso a diferentes
situações-problemas. Os alunos responderam um questionário para avaliação da atividade, onde
100% consideraram a dinâmica facilitadora para retenção do conhecimento. A experiência
mostrou que as técnicas de ensino centradas nos alunos são mais efetivas para o processo de
ensino-aprendizagem e motivadoras para ambos, professor e aluno.
10.003 - STRUCTURE OF ASCENDING PATHWAYS OF THE NERVOUS SYSTEM (EVAS): TEACHING
MODEL FOR LEARNING NEUROSCIENCES
GIOVANA M CANCIAN1; JOÃO LF BACCARELLI1; JOSÉ DE ANCHIETA DE CASTRO HORTA JÚNIOR2;
JULIANA IRANI FRATUCCI DE GOBBI3; MARCELO DM SASDELLI4; PEDRO T HAMAMOTO FILHO5; SILVIA
M NISHIDA3; TALITA HM SARTI4
1.MARÍLIA MEDICAL SCHOOL, FAMEMA, MARÍLIA, SP, BRASIL; 2.DEPT ANATOMY, INSTITUTE OF
BIOSCIENCE, UNESP, BOTUCATU, BOTUCATU, SP, BRASIL; 3.DEPT PHYSIOLOGY, INSTITUTE OF
BIOSCIENCE, UNESP, BOTUCATU, BOTUCATU, SP, BRASIL; 4.MARÍLIA MEDICAL SCHOOL , FAMEMA,
MARILIA, SP, BRASIL; 5.BOTUCATU MEDICAL SCHOOL, UNESP, BOTUCATU, SP, BRASIL.
In the teaching and learning process, the teacher-student relationship must be focused on students
as the main actors in the construction of knowledge. This paper is a case report in which a group
of students from the Marilia Medical School planned and developed an educational model of the
ascending pathways of the nervous system during the elective internship program at the Institute
of Biociences –UNESP. After the theoretical and practical study of neuroanatomy, neurophysiology
and clinical cases of Neurology, aimed at a meaningful learning about structure and function of
the nervous system and the deconstruction of a "neurophobic'' culture, it was proposed the
production of teaching materials as an evaluation method. The group chose to produce material
on the major ascending pathways. At the end of the internship program, they presented a didactic
model of the neuroaxis, made of wooden vertical boards simulating cross-sections of the spinal
cord, brainstem and forebrain.. Each plate was given stickers that represent internal structures
of the central nervous system (nuclei and bundles/tracts), and was perforated for the passage of
colored threads representing the ascending pathways, providing a three-dimensional visualization
of the morphological and functional organization of the sensory system. The EVAS represented an
innovative and facilitating experience of the teaching-learning process in neuroscience at the
undergraduate level.
10.004 - ACTIONS TO PROMOTE NEUROPHYSIOLOGY POPULARIZATION
BRUNA MEYER PERRONI; GEORGIA ELISA FILIPIN; MAUREN ASSIS DE SOUZA; LIANE DA SILVA DE
VARGAS; ALEXANDRE DOS SANTOS MARTINS; CAROLINE DALLA COLLETTA ALTERMANN; PAMELA
BILLIG MELLO CARPES
UNIVERSIDADE FEDERAL DO PAMPA, URUGUAIANA, RS, BRASIL.
The importance of popularize and disseminate basic concepts of neurophysiology is known. The
aim here is to report actions taken during the Brain Awareness Week (BAW) 2015 in
Uruguaiana/RS/Brazil. The activities occurred between 18 and 21 of March in a public park and
included four workshops: (i) Making art with the brain (drawing workshop); (ii) Neuroanatomy
exibhition; (iii) Neuromyths; and, (iv) Optical illusions. To evaluate the activities a questionnaire
was applied. 134 people participated in the activities, distributed in the workshops: (i) 38, (ii) 44,
(iii) 25 and (iv) 27, being 65 children/adolescents, 26 young adults, 43 adults and 5 elderly. Half
of the participants of the workshop (i) said that have already heard about neuroscience, however,
16 said to disregard the brain functions. Most participants of workshop (ii) (40) claimed to know
the structure of the brain and consider important to know about it. During the workshop (iii) we
realized that most participants (17) believed in neuromyths, however, after the discussions all
participants were able to understand how the fact neurophysiological processes occur.
Participants of workshop (iv) said that they have curiosity in understand optical illusions and
almost all (26) affirm that understood the role of the brain in optical illusions. In the end, the
participants attributed a final grade to each workshop: (i) 9,63; (ii) 9,79; (iii) 9,84; and, (iv) 9,81.
The actions accomplished their goal and popularize concepts of neurophysiology in the
community.
10.005 - SESSÃO DE INTEGRAÇÃO COMO METODOLOGIA ATIVA E FERRAMENTA DE AVALIAÇÃO
LUIS HENRIQUE MONTREZOR; KARIN FC PINOTTI; CYNTHIA M PIRATELLI
CENTRO UNIVERSITÁRIO DE ARARAQUARA - UNIARA, ARARAQUARA, SP, BRASIL.
A sessão de integração é realizada duas vezes por bimestre. É aplicada a todos os alunos do
primeiro ao oitavo módulos. Os alunos são organizados em grupos (de 10 a 15 alunos) com um
professor integrador para cada grupo. A atividade tem sessões de abertura e de encerramento.
Na abertura os alunos recebem um caso clínico relacionado às áreas temáticas do módulo que
cursam. Cada sessão tem um aluno coordenador e um relator. A sessão de abertura é composta
pela leitura do caso, determinação dos termos desconhecidos, dos dados relevantes, da discussão
do caso, das palavras-chave, da(s) hipótese(s) proposta(s) e da elaboração de objetivos para
resolução dos objetivos propostos. A sessão de encerramento é iniciada com a leitura do caso,
explicações dos termos desconhecidos, resolução dos objetivos propostos na sessão de abertura e
discussão para adequações da(s) hipótese(s) proposta(s) na abertura. Os alunos são avaliados
individualmente (em cinco ítens para cada sessão) pelo professor integrador em ambas as sessões.
A duração de cada sessão é de 2 horas. A participação dos alunos é continuamente estimulada
pelo aluno coordenador. Os resultados observados com tal metodologia são relevantes pois
percebe-se que os estudos realizados para as sessões antecipam os temas que serão abordados
nas aulas teóricas e nas avaliações. Além disso, a integração entre os alunos durante as discussões
dos casos prepara os mesmo para as discussões clínicas no hospital.
10.006 - THE PERCEPTION OF STUDENTS ABOUT THE USE OF A PORTFOLIO TO LEARN THE
PHYSIOLOGY OF THE ENDOCRINE SYSTEM
ALINE SOARES MIRANDA; MICHELLE FRANZ-MONTAN; FERNANDA KLEIN MARCONDES
FACULDADE DE ODONTOLOGIA DE PIRACICABA - UNIVERSIDADE ESTADUAL DE CAMPINAS,
PIRACICABA, SP, BRASIL.
The students of healthcare careers are expected to learn and to integrate the physiological
concepts about endocrine system. The objective of this study was to evaluate the perception of
student about the use of a portfolio to learn endocrine physiology. During physiology classes, in
2013 and 2014, 154 undergraduate students in Dentistry from Piracicaba Dental School have been
requested to prepare in pairs a portfolio. This portfolio should have basic information about
hormones action and secretion, relation with Dentistry, incorrect information found on the
internet, and answers to specific questions presented by the professor. The portfolio was used as
reference material to answer questions about clinical situations involving endocrine alterations in
class, and during the assessment of learning. At the end of the semester, all the students answered
the question: “The use of the portfolio was helpful in learning endocrine physiology? Why?”. For
142 students (92%), the portfolio was useful to enable them to understand the topics studied.
These students evaluated that during the preparation of the portfolio they were encouraged to
study alone and in groups, to know the importance of endocrine changes in dental practice. For
12 students (8 %) the portfolio was not useful because some students did not contribute in fact in
the group. According to these data the use of the portfolio as an approach has been considered
useful to learn endocrine physiology. In future studies, it will be evaluated if this practice in fact
improve the learning about endocrine physiology.
10.007 - PHYSIOLOGICAL UNDERSTANDING WEEK 2014: CONTRIBUTION OF COMMUNITY
EXTENSION ACTIVITIES IN PHYSIOLOGY LEARNING
CAROLINE DALLA COLLETTA ALTERMANN; BEN-HUR SOUTO DAS NEVES; RITHIELE GONÇALVES;
PAMELA BILLIG MELLO CARPES
The Physiological Understanding Week (PhUn Week) is an American outreach program that aims
building connections between scientists and society through the discussion of physiology contents.
So, we proposed to students enrolled in Human Physiology courses to organize community
activities to promote the physiology dissemination during the PhUn Week 2014 in
Uruguaiana/RS/Brazil. The activities were promoted between 03 and 07 of November. This work
aimed to evaluate the contributions of this propose in the development of students’ physiology
learning. 81 students divided into small groups carried out different activities with children, adults
and seniors in schools and parks. After the activities, the students answered a questionnaire. 98%
(n = 79) of the students considers that the proposal contributed to development of their learning,
skills and competencies and 91% (n = 74) considers it important for their education and their
understanding of physiology. In addition, the majority (86%; n = 70) said that it was possible to
increase the understanding of people in the community about the physiology and its importance
in their lives through the activities. Thus, the promoted outreach activities can be consider an
important tool to help the build of knowledge, to increase the students’ interest and to
disseminate important issues related to health and quality of life to community.
16
POSTER SESSIONS | MONDAY| AUGUST 3
THEME 01 - EXERCISE PHYSIOLOGY
01.020 - A PRIVAÇÃO DE SONO E O TREINAMENTO INTERVALADO DE ALTA INTENSIDADE
AUMENTAM A CONCENTRAÇÃO DE GLUCAGON
JORGE FERNANDO TAVARES DE SOUZA1; MURILO DÁTTILO1; MARCO TULIO DE MELLO2; HANNA
KAREN MOREIRA ANTUNES1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SANTOS, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DE MINAS
GERAIS, BELO HORIZONTE, MG, BRASIL.
Introdução: A privação do sono é uma condição deletéria para o metabolismo de carboidratos. Por
outro lado, a prática do exercício físico é uma boa estratégia para a melhora do metabolismo em
geral, e entre as diversas modalidades, o treinamento intervalado de alta intensidade (HIIT)
destaca-se por proporcionar benefícios em um curto espaço de tempo. Objetivos: Analisar os
efeitos da privação de sono e do HIIT sobre as concentrações de glucagon após 24 horas de privação
de sono. Métodos: Onze voluntários jovens do sexo masculino foram submetidos a 4 condições:
Sono Regular (SR), Privação de Sono (PS), HIIT + Sono Regular (HIIT + SR) e HIIT + Privação (HIIT +
PS). HIIT consistiu de 6 sessões de treino (8-12 tiros, 60s a 100% da potência máxima com 75s de
recuperação ativa) em bicicleta por 2 semanas. Após esse período, os voluntários dormiram ou
foram privados de sono por 24 horas consecutivas. Pela manhã, amostras de sangue foram
coletadas para avaliação da concentração de glucagon. Para a normalidade dos dados foi usado o
teste de Shapiro-Wilk, e para comparação da concentração de glucagon foi usado ANOVA one-way
com post hoc de Duncan e p≤0,05 (NCT02125656). Resultados: A concentração de glucagon foi
maior em HIIT + PS quando comparado às condições SR, PS e HIIT + SR, respectivamente.
Conclusão: A privação de sono não foi capaz de alterar a concentração de glucagon. Contudo, a
soma dos eventos estressores (HIIT e Privação de Sono) aumentou a concentração de glucagon.
01.021 - AUMENTO DO TÔNUS VAGAL DESENCADEADO PELO TREINAMENTO RESISTIDO
INFLUENCIA NA REDUÇÃO DO PERFIL INFLAMATÓRIO VENTRICULAR DE RATOS INFARTADOS
DANIELE JARDIM FERIANI1; MARCOS ELIAS ABSSAMRA1; JANAINA DE OLIVEIRA BRITO1; JULIANA
CRISTINA OLIVEIRA1; CATARINA DE ANDRADE BARBOZA1; LUIZA MELANIE SILVA1; LEANDRO YANASE
ROCHA1; NICOLLE MARTINS CARROZZI1; CRISTIANO MOSTARDA2; Mª CLAUDIA IRIGOYEN3; ERICO
CHAGAS CAPERUTO1; BRUNO RODRIGUES4
1.UNIVERSIDADE SÃO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DO
MARANHÃO, SAO LUIS, MA, BRASIL; 3.UNIDADE DE HIPERTENSÃO, INCOR, FMUSP, SAO PAULO, SP,
BRASIL; 4.FACULDADE DE EDUCAÇÃO FÍSICA, UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS,
SP, BRASIL.
A atuação do treinamento resistido (TR) dinâmico no reflexo anti-inflamatório desencadeado pelo
sistema nervoso parassimpático é pouco explorada. O objetivo deste estudo foi avaliar o TR na
função cardiovascular, autonômica e perfil inflamatório de ratos Wistar machos submetidos ao IM.
Os ratos foram divididos em grupos (n=10 cada): controle (C), controle + TR (CTR), IM sedentário
(IS), IM + TR (ITR). Os grupos treinados foram submetidos a 3 meses de TR dinâmico (40-60% do
teste de carga máxima (TCM) normalizado; 5 x semana). Fizemos avaliações ecocardiográficas,
TCM (pré e pós intervenção), hemodinâmicas, de sensibilidade barorreflexa, função autonômica
e concentração de citocinas inflamatórias no ventrículo esquerdo (pós). O TCM aumentou no grupo
ITR (455±8 g) em relação ao IS (245±9 g). A área de IM e a fração de ejeção não alteraram, mas a
espessura relativa de parede, a relação E/A e o índice de desempenho miocárdico melhoraram no
ITR (0,48±0,04; 2,07±0,10; e 0,33±0,02) em relação ao IS (0,28±0,02; 2,69±0,05; e 0,54±0,04).
Após o TR, o tônus simpático reduziu (50±4 bpm) e o tônus vagal aumentou (49±6 bpm) no ITR
quando comparado ao IS (64±6 e 35±5 bpm). As concentrações de TNF-α, IL-10 e a razão IL-10/TNFα ventriculares sofreram ajustes positivos no ITR (20,5±3; 12,7±1 e 0,46±0,07 pg/ug prot) em
relação ao IS (94,2±5; 38,5±5 e 0,38±0,02 pg/ug prot). Ocorreu correlação negativa entre o
tônusvagal e a concentração de TNF-α nos grupos avaliados (r=0,78; p<0,0001). Sendo assim,
sugere-se que o TR é benéfico no tratamento do processo inflamatório desencadeado pelo IM.
01.022 - PHYSICAL EXERCISE OR TAURINE SUPPLEMENTATION REDUCES OXIDATIVE STRESS AND
PREVENTS ENDOTHELIUM DYSFUNCTION IN HIGH-PALATABLE DIET-FED RATS
CAMILA DE MORAES1; LEANDRO KANSUKE OHAROMARI 2; NÁDIA FAGUNDES GARCIA1; ALINE ROSA
MAIA3; ANA PAULA DAVEL3; ELLEN CRISTINI DE FREITAS1
1.SCHOOL OF PHYSICAL EDUCATION AND SPORT, UNIVERSITY OF SAO PAULO, RIBEIRÃO PRETO, SP,
BRASIL; 2.DEPARTMENT OF INTERNAL MEDICINE, RIBEIRAO PRETO MEDICAL SCHOOL, UNIVERSITY
OF SAO PAULO, RIBEIRAO PRETO, SP, BRASIL; 3.DEPARTMENT OF STRUCTURAL AND FUNCTIONAL
BIOLOGY, UNIVERSITY OF CAMPINAS, CAMPINAS, SP, BRASIL.
Excessive consumption of high-fat and refined sugar lead to metabolic disorders as obesity and its
co morbidities. This study evaluated the effectiveness of physical exercise and Taurine
supplementation to prevent vascular and metabolic disorders caused by high-palatable diet
intake. Male Wistar rats (255-265g) were divided: sedentary; sedentary supplemented with 2%
taurine solution; trained and trained supplemented with 2% taurine solution. Exercise training
(treadmill at 60% maximal speed, 60min, 5 days/week) started after 4 weeks of high-palatable
diet-fed and was carried out for further 7 weeks. Sedentary rats had increased levels of insulin,
glucose and leptin. On the other hand, exercise effect only was seen to improve metabolic profile
lowering levels of leptin, insulin and glucose. Impaired relaxation was seen in sedentary group,
and it was improved not only by taurine supplementation but also by physical exercise. Increased
expression of EC-SOD was seen in aortas from all treated groups. Physical exercise, in absence of
taurine supplementation, was able to increase Cu-ZnSOD expression and reduce gp91phox.
Superoxide formation in aorta was lower either in supplemented or in trained groups. In summary,
not only physical exercise but also taurine supplementation were effective approaches to prevent
endothelial dysfunction induced by high-palatable diet-fed.
01.023 - GENE EXPRESSION PROFILE OF PROTEINS INVOLVED IN INFLAMMATION IN THE
COMMISSURAL NUCLEUS OF THE SOLITARY TRACT (CNTS) AND ROSTRAL VENTROLATERAL
MEDULLA (RVLM) IN SPONTANEOUSLY HYPERTENSIVE RATS (SHR) SUBMITTED TO SWIMMING
EXERCISE (EX)
ANDRÉA VANCETTO MAGLIONE1; ANDRESSA VENDRAMINI1; EDUARDO MAZUCO CAFARCHIO1; BIANCA
ALVES BIANCO1; ITATIANA FERREIRA RODART1; RUI MONTEIRO DE BARROS MACIEL2; LEANDRO DE
CARVALHO VALDO1; GISELE GIANNOCCO2; MONICA AKEMI SATO 1
1.FACULDADE DE MEDICINA DO ABC, SANTO ANDRE, SP, BRASIL; 2.FEDERAL UNIVERSITY OF SAO
PAULO, SAO PAULO, SP, BRASIL.
Aim: We investigated the gene expression profile of proteins involved in inflammation in the cNTS
and RVLM neurons in SHR submitted to Ex and sedentary (S) rats
Methods: Adult male SHR (14-16 wks-old, N=6-7/group) were submitted to daily Ex bouts (1h/day,
5days/wk) with 2% b.w. load for 6 wks or were maintained S. One day after the last exercise bout,
rats were anesthetized with 3% isoflurane in 100% O2, the brain stem was harvest and frozen in
liquid nitrogen for RT-PCR procedures later on. The m-RNA of cNTS and RVLM neurons was
extracted and the gene expression of cycloxygenase-2 (COX-2), interleukin 6 (IL-6), interleukin 10
17
(IL-10) and AT-1 receptor (AT-1r) was carried out by qPCR. Data was analyzed by Ct comparative
method and are as mean±SE, and submitted to unpaired Student t-test (p<0.05).
Results: Gene expression (g.e.) of COX-2 increased in Ex-SHR in cNTS (1.32±0.12 vs. 1.01±0.05S)
and RVLM (1.27±0.08 vs. 1.01±0.06S). The IL-6 g.e. reduced in RVLM in Ex-SHR (0.14±0.1 vs.
1.04±0.28S), while IL-10 g.e. increased in Ex-SHR (1.24±0.10 vs.0.98±0.05S). The AT-1r g.e.
decreased in Ex-SHR in cNTS (0.69±0.05 vs. 1.03±0.08S) and RVLM (0.65±0.04 vs. 1.02±0.08S).
Conclusion: The Ex induces adaptive responses in RVLM to reduce the oxidative stress dependent
on AT-1r in order to reduce the inflammatory response that could be augmented in SHR.
01.024 - LOW INTENSITY OF EXERCISE TRAINING IMPROVES CARDIOVASCULAR PARAMETERS IN
PULMONARY HYPERTENSION
RICARDO MEIRELLES BORBA1; FERNANDA TIZZIANI SCHNEIDER2; ANA AMÉLIA MACHADO DUARTE2;
JÉSSICA H.POLETTO BONETTO3; RAFAEL DE OLIVEIRA FERNANDES3; ALEXANDRE LUZ DE CASTRO3;
KATYA VIANNA RIGATTO3
1.UNISC/UFCSPA, PORTO ALEGRE, RS, BRASIL; 2.UFCSPA, PORTO ALEGRE, RS, BRASIL; 3.UFRGS,
PORTO ALEGRE, RS, BRASIL.
Pulmonary hypertension (PH) is characterized by increase in pulmonary vascular resistance,
leading to hypertrophy and failure of the right ventricle (RV), and autonomic nervous system (ANS)
dysfunction. There is a reduction in heart rate variability (HRV) and an imbalance of the renin
angiotensin system (RAS) increasing the vasoconstrictor axis. It remains unclear the ideal intensity
of aerobic exercise training (ET) in PH. We evaluate the effect of low and moderate ET on the
ANS and RAS enzyme expressions in the heart of PH-rats. PH was induced by a single injection of
monocrotaline (MCT; 50mg/kg). Groups: control (CO), MCT-sedentary (MCT-S), MCT-low intensity
(MCT-B), MCT-moderate intensity training (MCT-M). ET was performed on treadmill for 3 weeks.
ANS was analyzed by HRV and enzyme expressions by western blot (WB) in the RV. Data were
compared by two-way ANOVA follow by Tukey test (P<0.05). The RV weight and systolic pressure
(RVSP) were higher (P<0.05) in MCT-S vs CO. HRV was higher (P<0.05) in the ET-groups than in
sedentary groups (MCT-M and MCT-B vs MCT-S; MCT-B vs CO). Angiotensin converting enzyme (ACE)
expression was decreased (P<0.05) in ET-groups, but ACE2 was increased (P<0.05) in all groups vs
CO. Low intensity ET increases HRV, ACE2 expression, but attenuates RV hypertrophy, RVSP and
ACE expression. Collectively, our results indicate that the low intensity ET is more effective than
the moderated intensity to promote cardiovascular improvement in PH.
01.025 - AEROBIC EXERCISE TRAINING PREVENTS FEMORAL ENDOTHELIAL DYSFUNCTION IN
OBESE RATS
AMANDA CHRISTINE SPONTON1; HYGOR NUNES ARAUJO2; CARMEM PERES VALGAS DA SILVA2; CAMILA
DE MORAES3; EDSON ANTUNES1; ANGELINA ZANESCO2; MARIA ANDRÉIA DELBIN 1
1.UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL; 2.UNIVERSIDADE ESTADUAL
PAULISTA JULIO DE MESQUITA FILHO, RIO CLARO, SP, BRASIL; 3.UNIVERSIDADE DE SÃO PAULO,
RIBEIRÃO PRETO, SP, BRASIL.
The aim of the study was to investigate the effects of aerobic exercise training (AET) on femoral
artery responsiveness from rats fed with high-fat diet (HFD). Male Wistar rats were divided into:
control sedentary (c-SD); control trained (c-TR); HFD sedentary(h-SD); HFD trained(h-TR). For 16
weeks, h-SD and h-TR groups were fed with HFD. After 8 weeks, c-TR and h-TR groups initiated
the AET on treadmill (50-60% of maximum speed), 5 days/week, 60 min/session until the end of
the protocol. Concentration-response curves to acetylcholine (ACh), sodium nitroprusside (SNP),
phenylephrine (PHE) and the thromboxane A2 analogue (U46619) were obtained in femoral artery.
Vascular protein expressions were determined. Biochemical parameters were analyzed
systemically (CEUA:3203-1). The body weight, epididymal fat, triglyceride, insulin and leptin were
increased in h-SD group whereas AET reduced these alterations in h-TR group. The maximal
response to ACh was reduced in h-SD (62±4%) associated with decreased protein expressions of
eNOS, Cu/Zn-SOD and leptin receptor. The AET normalized the relaxation response in h-TR group
(73±1%) and also increased the expressions of eNOS, Cu/Zn-SOD and leptin receptor. No changes
were observed to SNP, PHE and U46619, as well as, in adiponectin receptor, Mn-SOD and EC-SOD
protein expressions. In conclusion AET for 8 weeks prevents femoral endothelial dysfunction in
rats fed with HFD and that was possible associated with increased nitric oxide production and/or
bioavailability.
01.026 - THE INTAKE OF ICE DOES NOT IMPROVE PHYSICAL PERFORMANCE DURING A HALFMARATHON IN THE HEAT
MARCELO TEIXEIRA DE ANDRADE1; WESLLEY MOURA SANTOS1; MATHEUS MASCARENHAS SACCHETTO
NUNES LEITE1; ADRIANO ARAÚJO LOBO DO CARMO1; DIOGO ANTONIO SOARES PACHECO1; NEIMAR
MARCOS DA SILVA1; YGOR ANTONIO TINOCO MARTINS1; NICOLAS HENRIQUE SANTOS BARBOSA1;
MOISÉS VIEIRA DE CARVALHO2; SAMUEL PENNA WANNER1; DANUSA DIAS SOARES1
1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSIDADE
ESTADUAL DE MINAS GERAIS, IBIRITE, MG, BRASIL.
Physical performance is reduced during prolonged exercise in the heat due to increased
thermoregulatory, cardiovascular and metabolic strain. Many cooling strategies have been
proposed to improve physical performance and reduce thermal strain, but the effects of ad libitum
intake of ice are still unknown. Thus, this study analyzed the effects of the ingestion of different
solutions (ice vs. water) in half-marathon performance. Ten men (27.8 ± 1.6 years; 60.2 ± 2.3
mLO2.kg-1.min-1) underwent four experimental trials, during which they should run 21.1 km as
fast as possible in a hot environment (33°C, 60% RH): ad libitum ingestion of ice at -1°C (Ad-1);
ad libitum ingestion of water at 37°C (Ad37); scheduled ingestion of ice at -1°C (Sch-1); scheduled
ingestion of water at 37°C (Sch37). The two ad libitum trials were first conducted in a random
order. During the Sch-1 and Sch37 trials, volunteers were induced to repeat the ad libitum intake
pattern but ingested a different solution (e.g. in the Sch-1, the volunteers ingested ice at the
same times and amounts that they ingested water in the Ad37). The 21.1 km running performance
was not affected by the different solutions that were ingested (Ad-1: 107.0±4.2; Ad37: 111.3±5.2;
Sch-1: 110.0±5.4; Sch37: 106.3±4.4 min). The ingestion of ice was not effective in changing the
thermoregulatory, cardiovascular and metabolic strain during exercise. We conclude that
ingestion of ice does not change performance in a half-marathon in the heat.
01.027 - ACUTE EFFECT OF STATIC STRETCHING CLASS ON POST EXERCISE HYPOTENSION AND
RATE PRESSURE PRODUCT
KAROLYNE SILVA MAGALHÃES; RAONI DA CONCEIÇÃO DOS SANTOS; CESAR RAFAEL MARINS COSTA;
WELINGTON VILLELA DE PAULA; WALLACE MARTINS VIANNA RIBEIRO; ANDERSON LUIZ BEZERRA DA
SILVEIRA
UFRRJ, RIO DE JANEIRO, RJ, BRASIL.
The stretching it has been much sought, but their effects are not well described in the literature,
so the purpose of this study was evaluate the acute effect of static stretching class on
cardiovascular responses. The sample was composed by 15 untrained and healthy young men.
After familiarization stretching class all subjects performed 45 minutes of 2 different protocols:
STRETC (performing an active static stretching class) and CTRL (remaining at rest) with 48h of
interval between them, in a cross-over design. The systolic (SBP), diastolic (DBP) and mean blood
pressure (MBP), heart rate (HR) and rate-pressure product (RPP) were measured before and
immediately after the protocols. The statistical procedure performed was two-way ANOVA and
the significance adopted was p≤0.05. We observed that before stretching class there was no
difference between protocols for all described variables (p>0,05). However, after stretching class
was found significant increase to SBP, MBP, HR and RPP between protocols. On the other hand,
there was not post exercise hypotension induced by STRETC during 25 min. at rest. These results
showed that stretching class can increase acutely blood pressure and cardiovascular stress, but
this exercise could not be able to induce post exercise hypotensive effect. Thus, stretching
exercise should be given with caution.
01.028 - THE EFFECTS OF ONE NIGHT OF PARADOXAL SLEEP DEPRIVATION (PSD) ON PHYSICAL
PERFORMANCE AND THERMOREGULATORY RESPONSES OF RATS SUBJECTED TO AN
INCREMENTAL EXERCISE UNTIL FATIGUE
FELIPE LIOE TEH SHANG; SAMUEL PENNA WANNER; LUCIANO SALES PRADO
This study evaluated the effect of one night of PSD on exercise time and thermoregulatory
responses of rats subjected to incremental exercises until fatigue in hot and temperate
environments. The study was approved by the local ethics committee (CEUA 273/2014). Nine male
Wistar rats were subjected to two incremental-speed, fatiguing treadmill running in a temperate
environment (24oC): normal sleep condition - control (C24) or after one night of PSD (PSD24); and
two incremental exercises in a hot environment (31oC): C31 and PSD31. The order of the trials
was randomized. The rats’ running time to fatigue were measured.
Sleep-deprived rats presented higher Trec prior to exercise, but similar rate of heat storage and
Trec at the end of exercise when compared to their respective control trials in both environments.
At 24C, sleep-deprived rats presented a lower exercise performance when compared to the
control trial, but no significant differences in performance were observed at 31C.
PSD reduces exercise performance in the temperate environment, but heat may represent to the
animals a higher physiological strain than sleep deprivation, so that no differences in performance
were observed between the two exercise trials in the heat. Higher Trec prior to exercise seem
not to influence final Trec, but may play a role in performance reduction in the temperate
environment.
01.029 - DRINK TEMPERATURE AFFECTS DRINKING BEHAVIOR OF ATHLETES DURING A SELFPACED RUNNING EXERCISE IN THE HEAT
MARCELO TEIXEIRA DE ANDRADE1; WESLLEY MOURA SANTOS1; MATHEUS MASCARENHAS SACCHETTO
NUNES LEITE1; ADRIANO ARAÚJO LOBO DO CARMO1; DIOGO ANTONIO SOARES PACHECO1; NEIMAR
MARCOS DA SILVA1; GUSTAVO GUIMARÃES AGUIAR DE OLIVEIRA 1; RÚBIO SABINO BRUZZI1; THIAGO
TEIXEIRA MENDES2; SAMUEL PENNA WANNER1; DANUSA DIAS SOARES1
FEDERAL DO MARANHÃO, SÃO LUÍS, MA, BRASIL.
Long distance races are popular and challenging sporting events, and many of them are performed
in hot environments. The aim of this study was to evaluate the pattern of voluntary intake of
drinks with different temperatures in runners during half-marathon in the heat. In a balanced and
randomized order, ten male volunteers (27.8 ± 1.6 years; 73.5 ± 2.6 kg; 178 ± 2 cm; 60.16 ± 2.3
mLO2.kg-1.min-1) were subjected to two experimental trials, during wich they ingested ice (-1ºC)
or water (37ºC) ad libitum, while running 21.1 km as fast as possible in a hot environment (33°C,
60%RH): The drink temperature influenced the pattern of voluntary intake in runners, as
evidenced by the difference in the total amount that was ingested (ice: 349.6 ± 58,5g vs. water:
635.5 ± 135.7g) and by the average intake per aliquot (ice: 66.5 ± 6.9 g vs. water: 118.6 ± 10.3 g;
p<0.05). However, the average number of aliquots that was ingested was not different between
the two experimental conditions (ice: 5.3 ± 0,8 vs. water: 5.1 ± 0.8) (p>0.05). We conclude that,
although the number of times that athletes seek for the ingestion of ice and water is similar, the
ice has a lower palatability than water during a self-paced running in the heat.
01.030 - THE EFFECTS OF FOUR NIGHTS OF PARADOXAL SLEEP DEPRIVATION (PSD) ON PHYSICAL
PERFORMANCE AND THERMOREGULATORY RESPONSES OF RATS SUBJECTED TO AN
INCREMENAL EXERCISE UNTIL FATIGUE
FELIPE LIOE TEH SHANG; GABRIELA CANGUSSU CASTRO; YGOR ANTONIO TINOCO MARTINS; SAMUEL
PENNA WANNER; LUCIANO SALES PRADO
This study evaluated the effect of four nights of PSD on physical performance and
thermoregulatory responses of rats subjected to incremental-speed exercises until fatigue in hot
and temperate environments.
The study was approved by the local ethics committee (CEUA 273/2014). Twelve male Wistar rats
were subdivided in two groups of 6 rats each. Both groups were subjected to an incrementalspeed, fatiguing exercise on a treadmill after 4 nights of PSD or under normal sleep conditions
(control). One group of rats exercised in both conditions (control – C24 and sleep deprived – PSD24)
in a temperate (24oC) environment while the other group exercised in both control (C31) and
sleep-deprived conditions (PSD31) in a hot (31oC) environment. The rats’ rectal temperature
(Trec) and running time to fatigue were measured.
Sleep-deprived deprived rats presented higher Trec prior to exercise, similar rate of heat storage
and lower performance when compared to their respective control trials in both environments.
Trec at the end of exercise was different between control and sleep-deprived rats only in
temperate environment.
PSD reduces exercise performance and changes the thermoregulatory responses of running rats in
both temperate and hot environments. The altered thermoregulatory response in sleep-deprived
rats can explain their lower running time only in the heat.
01.031 - THE EFFECTS OF ELECTRICAL STIMULATION ON THERMOREGULATORY RESPONSES AND
PERFORMANCE OF RATS SUBJECTED TO A PHYSICAL EXERCISE
WILLIAM COUTINHO DAMASCENO; FILIPE SALVADOR ZINATELLI COELHO; ANA CANCADO
KUNSTETTER; SAMUEL PENNA WANNER
Electrical stimulation is commonly used to encourage rats to run on a treadmill. This study
evaluated the effects of electrical stimulation on thermoregulatory responses and performance of
rats subjected to a physical exercise.
Seven adult male Wistar rats (250–350 g) were used in the experiments. Under anesthesia, a
temperature sensor was implanted in the abdominal cavity of the rats. After four days of recovery,
the rats were familiarized with the treadmill running (5-day protocol, electrical stimulation on)
and then subjected to two experimental trials at 24°C: incremental-speed running (starting at 10
m/min, with increases of 1 m/min every 3 min) until volitional fatigue, with or without electrical
stimulation. The abdominal temperature (Tabd) and tail skin temperature (Ttail) were measured
during the exercise.
In both experimental trials, the treadmill running increased Tabd and Ttail 8 and 16 min after the
exercise had initiated, respectively. During the initial moments of exercise, there were no
differences in Tabd and Ttail between the experimental trials. However, the animals subjected
to electrical stimulation ran more and achieved higher Tabd and Ttail at volitional fatigue
compared with animals non-subjected to electrical stimulation.
The electrical stimulation does not affect thermoregulatory responses during the initial moments
of exercise, but increases physical performance and consequently the Tabd and Ttail at volitional
fatigue.
01.032 - COMPOSIÇÃO CORPORAL E RENDIMENTO FÍSICO: ALTERAÇÔES PRODUZIDAS PELO
EXERCÍCIO FÍSICO EM RATOS JOVENS TRATADOS COM A DIETA RICA EM CARBOIDRATO SIMPLES
MARIANA ARAÚJO VIEIRA DO CARMO; ANGÉLICA BARBOSA GONÇALVES PINTO; ÉLIDA MERCÊS
RAIMUNDO; LENICE KEPPES BECKER
UNIVERSIDADE FEDERAL DE OURO PRETO, OURO PRETO, MG, BRASIL.
Objetivo: Analisar o efeito do treinamento físico sobre as alterações produzidas pela dieta rica
em carboidrato simples (DCS) em ratos jovens. Metodologia: Ratos Wistar com 21 dias foram
submetidos a 4 (T4) e 8 semanas (T8) de treinamento em natação com sobrecarga, 1 hora/dia, 5
vezes/semana. Os ratos dos grupos sedentário não realizaram exercício (SE4 e SE8). Foi feito um
teste de carga máxima (TCM) pré e pós-treinamento para avaliar o rendimento físico. O Índice de
Lee (IL) e o Índice de adiposidade corporal (IAC) foram calculados no dia do sacrifício. Os dados
foram expressos como média ± desvio padrão. Foram utilizados o teste ANOVA, seguido de pós
teste de Bonferroni e teste Kruskal Wallis, seguido de pós teste de Dunns. Resultados: Não houve
diferença no delta de tempo de exercício no TCM entre os grupos SE4 (-3,7 ± 2 minutos) e T4 (3,4 ± 2,3 minutos), no entanto, houve diferença significativa entre os grupos SE4 (-3,7 ± 2 minutos)
e T8 (0,06 ± 4 minutos). O IL não indicou diferença (SE4 1,7 ± 0,05 vs T4 1,7 ± 0,1). Após 8 semanas,
T8 (1,7 ± 0,04) mostrou menor IL em comparação com SE8 (1,8 ± 0,11). O IAC não diferiu entre os
grupos (SE4 2,3 ± 0,26 % vs T4 2,6 ± 0,51 %; SE8 2,2 ± 0,44 % vs T8 2,1± 0,81%). Conclusão: Os
18
resultados sugerem que a associação entre DCS e exercício físico, promove alterações no
rendimento físico que são dependentes do tempo de treinamento.
01.033 - CORRELATIONS AMONG THE CHANGES IN MUSCLE, CORE AND SKIN TEMPERATURES
DURING EXPOSURE TO A COLD ENVIRONMENT
GUSTAVO GUIMARÃES AGUIAR DE OLIVEIRA1; CHRISTIAN EMMANUEL TORRES CABIDO1; DIEGO
CAMPOS DE MOURA1; NATHÁLYA GARDÊNIA DE HOLANDA MARINHO NOGUEIRA1; THIAGO FONSECA
DOS SANTOS1; MATHEUS MILANEZ DOS REIS1; EMERSON SILAMI GARCIA2; SAMUEL PENNA WANNER1;
MAURO HELENO CHAGAS1
1.UFMG-MG, BELO HORIZONTE, MG, BRASIL; 2.UFMA, SÃO LUIZ, MA, BRASIL.
The purpose of this study was to verify the association level among the muscle temperature (MT)
measured in vastus lateralis (VL), rectal temperature (RT) and skin temperature (ST) during
exposure to a cold environment (10C;60 min). Six male volunteers (age=27.0±3.3 years; body
mass=76.5±11.5 kg: average ± DP) were analyzed. An ultrasonography device was used to measure
the thickness of the VL, which was divided by two and then summed to subcutaneous tissue; this
calculation allowed us to determine the depth at which the sensor was inserted. Next, the VL area
was sterilized and a cannula (18G) was inserted in the thigh of the subjects. The temperature
sensor was inserted in the muscle through the guide cannula and, after 30 min of rest, the
volunteers were directed to an environmental chamber, where they remained seated, wearing
only shorts, socks and sneakers. The Pearson’s correlation coefficient was applied to evaluate the
association level among MT, RT and TS during the 60 min of exposure. The cold exposure decreased
body core, muscle and skin temperatures by 37.02±0.25 to 36.76±0.40C, 33.70±1.37 to
29.94±1.80C and 28.02±0.93 to 22.95±1.39C, respectively. The results also indicated significant,
high and positive correlations between MT and RT (r=0.89;p<0.05) and MT and TS (r=0.91;p<0.05).
In conclusion, although the magnitude of the temperature responses of these compartments is
different, these responses to changing external temperatures are interconnected.
01.034 - TRANSGENIC RATS WITH INCREASED CIRCULATING ANGIOTENSIN-(1-7) HAVE LESS
MUSCLE DAMAGE AFTER EXERCISE TO EXHAUSTION
NÁDIA LÚCIA TOTOU1; MARIANA FLÁVIA OLIVEIRA2; LENICE KEPPES BECKER1
1.UNIVERSIDADE FEDERAL DE OURO PRETO, OURO PRETO, MG, BRASIL; 2.UNIVERSIDADE FEDERAL
DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL.
Angiotensin-(1-7) [Ang-(1-7)] Ang-(1-7)/Mas axis plays a critical role in muscle damage in different
experimental models. Purpose: The aim was investigate the influences of higher circulating levels
of Ang-(1-7) on muscle damage markers after physical exercise. Methods: Sprague–Dawley (SD)
and in TGR(A1-7)3292 (TG) rats which present approximately two fold increase in plasma Ang-(17) levels compared to SD were submitted to exercise until exhaustion with a weight equivalent to
4% of body weight tied to the tail. Blood samples were taken to analyze the creatine kinase (CK),
lactate desidrogenase (LDH), and α-actin levels on skeletal muscle. Student t test was used to
compare possible differences between groups. Results: Total exercise time was not different
between groups TG (5.1 ±0.3 hours) and SD (4.8 ± 0.3 hours). The results obtained to muscle
damage markers show that LDH was lower in TG rats 158±19 U/L in comparison with SD rats 211±10
U/L. Equally, α-actin was lower to TG 224 ±29 U/L in comparison with SD rats 360 ±55 U/L, CK
values was not different between groups 468 ± 16 U/L to TG vs 462 ±13 to SD group. Conclusion:
This study shows that Ang-(1-7) can be effective in prevent the muscle damage induced by exercise
until exhaustion.
01.035 - SYMPATHO-VAGAL CHANGES INDUCED BY EXERCISE TRAINING IN AGEING
SPONTANEOUSLY HYPERTENSIVE RATS
LAÍS OLIVEIRA DELLACQUA1; CHARLIE HINDMARCH2; JULIAN FRANCIS RICHMOND PATON2; DAVID
MURPHY2; LISETE COMPAGNO MICHELINI1; VAGNER ROBERTO ANTUNES1
1.USP, OSASCO, SP, BRASIL; 2.UNIVERSITY OF BRISTOL, BRISTOL, REINO UNIDO.
The practice of physical exercise is related to an improvement of hypertensive damages, because
it leads to an increase in parasympathetic tone as well as a decrease in sympathetic tone, leading
to a reduction in blood pressure. The aims of this study were to evaluate the sympathetic-vagal
balance and hemodynamic components in exercise-trained (T) ageing SHR. SHR and WKY rats, 12months-old were trained 5 days/week for 8 weeks. SHR (T8) had a significant reduction in the SBP
(155±11 mmHg, n=7) when compared to the sedentary (S8) group (198±10 mmHg; n=7, p<0.05). In
addition, the spectral analysis of the heart rate variability have shown a higher HF index of SHR
(T8) (22.4±3.4; n=5) compared to the S8 (6.3± 2.4; n=5). The LF/HF ratio was lower in SHR trained
for 8 weeks (0.21 n=5) when compared with control animals sedentary (0.43 n=5), an indicative
of higher parasympathetic tone on these animals. Collectively, our data show that physical
exercise for 8 weeks in ageing SHR elicits benefits in the cardiovascular system by reducing systolic
blood pressure and increasing in the parasympathetic tone to the heart.
01.036 - TRANSGENIC RATS WITH INCREASED CIRCULATING ANGIOTENSIN-(1-7) HAVE BETTER
METABOLIC PROFILE AFTER EXERCISE TO EXHAUSTION
ANGÉLICA BARBOSA GONÇALVES PINTO1; MARIANA FLÁVIA OLIVEIRA2; LENICE KEPPES BECKER1
1.UNIVERSIDADE FEDERAL DE OURO PRETO, OURO PRETO, MG, BRASIL; 2.UNIVERSIDADE FEDERAL
The Angiotensin-(1-7) [Ang-(1-7)] plays a critical role in metabolic pathways involved in glucose
control. The aim of this study was investigates the influences of higher circulating levels of Ang(1-7) on glucose responses and glycogen content after extraneous physical exercise. Methods:
Sprague–Dawley (SD) and in TGR(A1-7)3292 (TG) rats which present approximately two fold
increase in plasma Ang-(1-7) levels compared to SD were submitted to exercise until exhaustion
with a weight equivalent to 4% of body weight tied to the tail. Blood samples were taken to
analyze the glycemic response to exercise; liver and muscle tissue glycogen content were
evaluated. Student t test was used to compare possible differences between groups. Results:
Despite the total exercise time was not different between groups TG (5.1 ±0.3 hours) and SD (4.8
± 0.3 hours) the exercise induced a lower decrease in glucose serum levels in TG (-13±10 mg/dl)
rats in comparison with SD rats (-41±7 mg/dl). TG rats were able to save glycogen content; the
content of TG rats was higher after exercise in muscle (0.12 ±0.02 mg/g) and liver (2.24±0.8 mg/g)
when compared with SD rats (0.06±0.0009 mg/g to muscle, 0.65±0.13 to liver). Conclusion: This
study shows that Ang-(1-7) has important role in metabolic control during exhaustive exercise.
01.037 - O EXERCÍCIO FÍSICO EM MEIO AQUÁTICO SENSIBILIZA MAIS O BARORREFLEXO EM
COMPARAÇÃO COM MEIO TERRESTRE EM RATOS ESPONTANEAMENTE HIPERTENSOS
ÉLIDA MERCÊS RAIMUNDO; MARIANA ARAÚJO VIEIRA DO CARMO; LENICE KEPPES BECKER
UFOP, OURO PRETO, MG, BRASIL.
O treinamento físico em meio aquático produz respostas fisiológicas distintas das observadas em
meio terrestre. Objetivo: comparar o efeito do exercício físico em meio aquático e terrestre sobre
a sensibilidade barorreflexa em ratos espontaneamente hipertensos (SHR). Metodologia: Ratos
SHR foram submetidos a 8 semanas de treinamento físico em piscina (PIS) e esteira (EST). Ao final
das 8 semanas, os animais foram anestesiados por via intraperitoneal, para canulação da artéria
femoral para registro da pressão arterial média (PAM) e veia femoral para administração de
fenilefrina (FE, 8µg/ml). Os dados foram expressos em média ± erro padrão da média. Resultados:
O teste de carga máxima mostrou que houve aumento do rendimento físico para os grupos PIS
(6±3 minutos) e EST (8±2 minutos) em comparação com o grupo sedentário (-1±1 minuto). A
resposta da PAM à fenilefrina não foi diferente entre os grupos EST (54 ± 6mmHg) e PIS
(56±9mmHg), porém a resposta bradicardica a FE foi significativamente maior para o grupo PIS (109 ± 26 bpm) em comparação ao grupo EST (-54±14 bpm) Conclusão: Estes dados mostram que o
meio onde o exercício físico é realizado pode modular as respostas do barorreflexo em ratos SHR.
01.038 - INFLUENCE OF NEONATAL MATERNAL SEPARATION ON PHYSICAL PERFORMANCE OF
RATS DURING PROGRESSIVE EXERCISE
JULIANA BOHNEN GUIMARÃES1; FREDERICO SANDER MANSUR MACHADO2; DANIELA COSTA ROCHA
FÓSCOLO2; PAULO MARCELO A. LIMA2; CÂNDIDO CELSO COIMBRA2
1.UNIVERSIDADE DO ESTADO DE MINAS GERAIS, IBIRITÉ, MG, BRASIL; 2.UNIVERSIDADE FEDERAL DE
MINAS GERAIS, BELO HORIZONTE, MG, BRASIL.
In childhood, maternal cares are the main sources of stimuli for proper development, growth and
adaptive responses to stressors. Deprivation of maternal presence can break this protection and
promotes alterations in metabolism due to change in neuronal program. Thereafter, the main was
to verify if maternal separation (MS) during the first days of life interferes with thermoregulation
and consequently the physical performance in rats during exercise.
On the 14th day of postnatal life, the litters were maternal separated (MS) which consisted in
removing the mother from the cage for 180 min, while leaving the pups undisturbed. The control
animals were left undisturbed throughout this period. After two months from weaning, it was
implanted an intraperitoneal sensor in order to measure the core body temperature (Tc). After
recovery, rats underwent a progressive exercise (PE) in a treadmill (the velocity was increased by
1 m/min in each 3 minutes - initial speed 10 m/min), until fatigue. MS reduced exercise time,
maximal velocity and workload (P < 0.05). PE induced an increase in Tc in both groups. Tc at
exercise onset and fatigue was not different between groups (fatigue – C: 38.4 ± 0.4°C vs. MS:
38.5 ± 0.5°C; P = 0.5). From these data, it was calculated the heat storage rate which showed
lower values in C compared to MS (P < 0.05). As conclusion, MS reduces performance during PE
until fatigue. In addition, heat storage rate could have contributed to this result. These results
point to the importance of the early maternal care for future development of individuals.
01.039 - AEROBIC EXERCISE RESTORES CENTRAL ACTION OF INSULIN IN SPONTANEOUSLY
HYPERTENSIVE RATS (SHR)
IZABELA MARTINA RAMOS RIBEIRO; LAÍS OLIVEIRA DELLACQUA; ANA CAROLINA PANVELOSKI-COSTA;
NEWTON SABINO CANTERAS; VAGNER ROBERTO ANTUNES
USP, SÃO PAULO, SP, BRASIL.
Insulin is an important hormone in the control of glucose production at the hepatic territory, but
little is known about its action on modulation of autonomic nervous system (ANS) in the control
of glicemia in hypertension, usually associated to insulin resistance and metabolic dysfunction.
Previous studies from our laboratory demonstrate significant impairment in the central action of
insulin in modulating hepatic glucose production in SHR (IMR Ribeiro et al, 2015). The present
study shows that aerobic exercise training for 4 weeks (T4) promotes a significant fall in hepatic
vein blood glucose levels at 5 minutes after insulin injection (100nU/1ul) in the lateral ventricle
(LV) in 18 weeks old SHR animals (-18% at 5 min, -24% at 10 min, with a maximum decreasing at
30 min (23%, n=6) compared at the same time course to the sedentary (S) SHR group (0% at 5 min,
4% at 10 min, and 10% at 30 min after central injection of insulin; n=6). However, there was no
significant difference in peripheral insulin sensitivity in the T4 (increases 13%) compared to the
sedentary group, demonstrated by rate constant for insulin tolerance test (kITT). Our results
suggest that physical exercise promotes an improvement in central action of insulin in SHR, which
is suppose to be due to amelioration of central sensitivity of this hormone, since its peripheral
action was not affected by aerobic exercise.
THEME 02 - RENAL PHYSIOLOGY
02.009 - INSULIN, ALBUMIN AND GLUCOSE DOWNREGULATE GLUT2 PROTEIN EXPRESSION IN
LLC-PK1 PORCINE PROXIMAL TUBULE CELL LINE
JENIFFER PEREIRA DA CRUZ GONCALVES
UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, MACAE, RJ, BRASIL.
Until now the role of albumin, glucose and insulin in the regulation of GLUT2 protein expression
in kidney proximal tubule is not well known. The aim of this work was evaluate cell viability and
GLUT2 expression in LLC-PK1 under insulin, albumin and glucose treatments. The cells were
treated 24h with starved medium (CTRL) or starved medium supplemented with insulin (ng/ml 5, 50 and 500), albumin (mg/ml - 0.01, 0.1, 1, 10 and 20) and glucose (mM - 5, 10, 20 and 40).
Cell viability was determined using MTT assay and GLUT2 protein expression was determined using
Western Blot. Statistical analysis were performed using One-Way ANOVA followed by Dunnet postest (mean ±SEM, p < 0.05). It was observed that relative GLUT2 expression (times of control) was
lower in cells treated with insulin at 5, 50, 500 (0.84±0.037, 0.81±0.045 and 0.72±0.046), glucose
at 10, 20 and 40 (0.46±0.040, 0.57±0.080 and 0.65±0.12) and albumin at 0.1, 1 and 10 (0.74±0.059,
0.74±0.052 and 0.71±0.078), compared to CTRL (1.00 ± 0, n = 4, p < 0.05). The cell viability was
higher in cells treated with insulin at 50 and 500 (1.4±0.10 and 1.5±0.14) and albumin at 10 and
20 (1.45±0.058 and 1.88±0.089) compared to CTRL (1.0±0, n = 12, p < 0.05). No significant changes
in cell viability was observed after high glucose concentration treatment. High concentrations of
albumin, insulin and glucose are able to downregulate GLUT2 expression and only insulin and
albumin are able to increase cell viability.
02.010 - PAPEL DO RECEPTOR EXTRACELULAR PARA CÁLCIO (CASR) NA REGULAÇÃO DA H+ATPASE PRESENTE EM CÉLULAS DO NÉFRON DISTAL
ANNA GABRIELLE COUTINHO; RICARDO FERNANDEZ PEREZ
UNIVERSIDADE FEDERAL DO PARANÁ (UFPR), CURITIBA, PR, BRASIL.
Vários fatores tem sido identificados como reguladores diretos e indiretos da atividade da H +
ATPase vacuolar presente nos segmentos distais do néfron, dentre eles: íons como Na+ , K+ e Ca++;
e hormônios como ADH e PTH, Angiotensina II e Aldosterona. Num estudo recente do nosso
laboratório utilizando porções de membrana plasmática parcialmente purificadas da região
cortical e medular externa do rim de camundongo foi observado que o estímulo do receptor
sensível a cálcio, o CaSR, pelos agonistas Gd3+ ou neomicina incrementou de forma significativa a
atividade bioquímica da H+ ATPase do tipo vacuolar e da H+ /K+ -ATPase do tipo gástrica. O objetivo
do presente estudo é investigar a interação do CaSR com a atividade da H + ATPase num modelo
de células renais do ducto coletor de mamíferos, as células MDCK. A atividade bioquímica da H +
ATPase foi determinada em um homogenado de células MDCK por um método colorimétrico
modificado do descrito por Fiske-Subarow, onde a diferença entre o Pi liberado na ausência e na
presença de concanamicina 10-8M corresponde à atividade da mesma. Os resultados parciais
apontam que a estimulação do CaSR com neomicina 300μM ou Gd 3+ 200μM incrementa a atividade
da H+ ATPase vacuolar: 31,1±9,9nmol Pi.mg -1.min-1 (n= 9) atividade basal, 53±15,2nmol Pi.mg1
.min-1 (n= 8) com neomicina e 44,7±9nmol Pi.mg-1.min-1 (n= 9) com Gd3+.
02.011 - PRESSÃO ARTERIAL E PARÂMETROS DA FUNÇÃO RENAL DE RATOS WISTAR DE NINHADA
REDUZIDA ORIUNDOS DE FÊMEAS EXERCITADAS DURANTE A GESTAÇÃO E LACTAÇÃO
LETICIA DINIZ CREPALDI
A alta prevalência da obesidade infantil e adulta envolvem fatores relacionados ao estilo de vida,
tais como: grande consumo energético e baixos níveis de atividade física. Em ratos, a redução do
tamanho da ninhada é um modelo de indução de sobrepeso/obesidade. Considerando esse
contexto, o presente trabalho teve como objetivo analisar parâmetros da função renal e pressão
arterial média (PA) da prole submetida à redução de ninhada, oriundas de ratas exercitadas em
esteira (30% VO2max) durante a gestação e lactação. Os animais foram divididos em dois grupos:
G-CON (controle mãe exercitada – n=8) e G-RN (redução de ninhada mãe exercitada – n= 9). Aos
90 dias de vida, foi realizado registro da pressão arterial média, coleta de sangue, medida da
ingestão hídrica, coleta da urina, análise da proteinúria e da taxa de filtração glomerular (TFG).
Não houve alteração significativa na PA (G-CON= 101,31 ± 12,53 e G-RN= 94,35 ± 7,76 mmHg), no
fluxo urinário (G-CON =0,02 ±0,008 e G-RN = 0,033 ± 0,02mL/min), na proteinúria (G-CON = 3,93
±2,7 e G-RN = 6,07 ±2,06mg/13h) e na TFG (G-CON = 0,9 ±0,4 e 0,7 ±0,2mL/min/100gpc) da prole
submetida à redução de ninhada. Portanto, o exercício físico de baixa intensidade (30% VO2max),
durante a gestação e lactação, não alterou pressão arterial, taxa de filtração glomerular e
proteinúria da prole submetida à redução de ninhada.
02.012 - AEROBIC EXERCISE PREVENTS ACUTE KIDNEY INJURY
ESDRAS GUEDES FONSECA1; MARKUS BERGER2; LEDA MARIA DE CASTRO COIMBRA CAMPOS1;
ROBERTA DA SILVA FILHA1; TATIANE ALMEIDA1; MARIA APARECIDA RIBEIRO VIEIRA1
1.UFMG, BELO HORIZONTE, MG, BRASIL; 2.UFRGS, PORTO ALEGRE, RS, BRASIL.
There are few studies reporting the possible kidney adaptations induced by aerobic exercise (AE),
especially in acute kidney injury (AKI). In this study, we investigated the effect of aerobic exercise
of moderate intensity, on the prevention of AKI induced by gentamicin (GM). The whole procedure
was approved by the Ethics Committee on Animal Experiments (CETEA No 182/08). Before the
19
start of training, 28 Wistar rats (250-300 g) underwent a progressive maximal exercise test (TEMP).
Soon after, the animals were divided into two groups: not trained (NT) and trained (T). The
training protocol consisted of group T treadmill running at moderate intensity at 65% of maximum
speed achieved in TEMP (60 min / day, 5 days / week for 8 weeks). After the training period, the
rats were placed individually in metabolic cages. The two groups rats were divided into subgroups
and treated subcutaneously with saline(SAL,0.9% NaCl); NT-SAL and T-SAL and
gentamicin(GM,Gentatec ®, 80 mg / kg / day, 0.1 ml/100g); NT-GM and T-GM for 5 days. Urine
and blood samples were collected before and after the treatment, for analysis of renal function.
The AE prevented the decrease in glomerular filtration rate (GFR) induced by GM. Separately, GM
and AE produced increased kidney expression of RNA for ACE. The previous PT prevents the
increase of the expression of ACE and reduced expression of AT1 receptors. These data indicate
that AE prevents the kidney damage induced by GM, possibly because of a minor activation of the
vasoconstrictor axis of angiotensin system.
02.013 - CHRONIC FISH OIL SUPPLEMENTATION REVERTED PARTIALLY RENAL ALTERATIONS ON
MICE FED WITH A HIGH-FAT DIET
ISABELA COELHO DE CASTRO1; DANIELLE CRISTINA TONELLO PEQUITO2; GINA BORGHETTI3;
ADRIANA AYA YAMAGUCHI4; GLEISSON A. P. DE BRITO5; LUIZ CLAUDIO FERNANDES6; TEREZILA
MACHADO COIMBRA7; RICARDO FERNANDEZ PEREZ6
1.UFLA, LAVRAS, MG, BRASIL; 2.UFMS, TRÊS LAGOAS, MS, BRASIL; 3.UFRR, BOA VISTA, RR, BRASIL;
4.INSTITUTO DE PESQUISA PELÉ PEQUENO PRÍNCIPE, CURITIBA, PR, BRASIL; 5.UNIVERSIDADE
FEDERAL DA INTEGRAÇÃO LATINO-AMERICANA (UNILA), FOZ DO IGUAÇU, PR, BRASIL; 6.UFPR,
CURITIBA, PR, BRASIL; 7.FMUSP-RB, RIBEIRÃO PRETO, SP, BRASIL.
The present work was evaluated the effect of chronic fish oil (FO) supplementation on renal
morphology and function of mice fed with a high-fat diet (HFD). Six weeks male mice were divided
in two groups and fed during eight weeks with regular chow (R), or HFD. After this, they were
supplemented orally for 30 days with FO, 1g/kg body weight. Renal function parameters, urinary
excretion of TXB2, PGE2 and albumin, immunolocalization of vimentin, desmin were measured.
The weight gain of HFD group was higher, almost 2 times, than R group. HFD group presented
glucose intolerance, an effect that was not modified by FO. Creatinine clearance was lower in
HFD (0.81±0.09 mL/min/kg body wt) when compared to R (1.28±0.22) (p<0.05), independent of
FO supplementation. HFD induced albuminuria: 35.65±7.32ng/mg in HFD vs 11.06±2.16 in R
(p=0.01). FO supplementation reduced in 40% albumin excretion in HFD. Urinary TXB2 excretion
was reduced in 58% by high-fat diet and FO was not able to reverse this modification. Urinary
PGE2 excretion was similar among groups. The HFD was increased in almost 100% the
concentration of TNF-α in renal tissue; an effect reverted by FO supplementation. The intrarenal
expression of vimentin was significantly higher in tubulointerstitial cells of HFD group, an effect
reverted by FO. Desmin expression in glomerulus edge increased in obese animals, but was not
modified by FO supplementation. In conclusion, FO reverted partially renal alterations induced by
a HFD
02.014 - SLEEP RESTRICTION DURING PREGNANCY ALTERS RENAL FUNCTION IN FEMALE RAT
OFFSPRING
ROGÉRIO ARGERI1; GUIOMAR NASCIMENTO GOMES2; SERGIO TUFIK 3
1.UNIFESP, SÃO PAULO, SP, BRASIL; 2.PROFESSORA DO DEPARTAMENTO DE FISIOLOGIA UNIFESP/EPM, SÃO PAULO, SP, BRASIL; 3.PROFESSOR DO DEPARTAMENTO DE PSICOBIOLOGIA - UNIFESP/EPM, SÃO PAULO, SP, BRASIL.
The reduction of sleeping time leads to hormonal and metabolic alterations. Moreover, changes
in maternal environment may modify the development of the offspring leading to development of
diseases at adulthood. Objectives: Evaluate renal function of female offspring from rats sleep
restricted during the last week of pregnancy. Methods: Pregnant Wistar rats were divided in 2
groups: control and sleep restricted. The sleep restriction was carried out between 14th and 20th
day of pregnancy, 20 h/day. Female offspring: Control (C, N=17) and Sleep Restriction (SR, N=26)
were studied at 8 months old. Parameters studied: indirect BP (BPi – tail cuff), urea ([P]u) and
creatinine ([P]cr) plasma concentration, creatinine clearance (CLcr), Na+ excretion (CENa+), urine
flow (V), protein excretion (PT-ur), number of glomeruli per field (Nglo) and renal mass (Rmass).
Data showed as mean±SEM, P≤0.05, t-student. Results: SR group presented increased values of BPi
[C: 124.9±0.7; SR: 130.8±0.5 mmHg]; smaller CLcr [C: 4.6±0.3; SR: 3.4±0.2 ml/min/kg]; increased
PT-ur [C: 5.0±0.4; SR: 6.5±0.5 mg/24h], and reduced Rmass [C: 0.7±0.02; SR: 0.6±0.01 g/100g
bw]. There were no significant differences in the others parameters. Conclusion: Sleep restriction
during pregnancy changed renal development leading to alterations in renal function and in blood
pressure in offspring at later life.
02.015 - EVALUATION OF VASOPRESSIN IN SITU ADMINISTRATION IN URINARY BLADDER AND
GENE AND PROTEIN EXPRESSION PROFILE OF VASOPRESSIN RECEPTOR SUBTYPES IN FEMALE
WISTAR RATS
EDUARDO MAZUCO CAFARCHIO1; ITATIANA FERREIRA RODART1; BIANCA ALVES BIANCO1; GISELE
GIANNOCCO2; RUI MONTEIRO DE BARROS MACIEL2; DANIEL PAULINO VENÂNCIO1; MONICA AKEMI
SATO1
1.FMABC, SANTO ANDRE, SP, BRASIL; 2.UNIFESP, SÃO PAULO, SP, BRASIL.
Aim: This study investigated the effects of in situ vasopressin administration in different doses in
urinary bladder and gene and protein expression of vasopressin receptors.
Materials and Methods: Female Wistar rats (~250 g, N= 6/group) were anesthetized with 2%
isoflurane in 100% O2, the urinary bladder was cannulated to measure the intravesical pressure
(IP). After the baseline recording, it was administrated approximately 0,1 mL the vasopressin at
different concentrations (1 ng/mL, 0,5 ng/mL and 0,25 ng/mL) or saline on urinary bladder. Data
are as mean±SE and were submitted to unpaired Student t-test (p<0.05). Separated groups of
animals were used to qPCR and western-blot, respectively, in order to evaluate the vasopressin
receptor subtypes (V1a, V1b and V2).
Results: The vasopressin increased the IP (107±3, 59±4 e 56±4% in the doses of 1ng/mL, 0,5ng/mL
e 0,25ng/mL, respectively) compared to saline (2±1%). We observed the gene and protein
expression of all subtypes of vasopressin receptors in the urinary bladder
Conclusion: In situ administration of vasopressin increased the IP, demonstrating the existence of
vasopressin receptors in urinary bladder as either the gene and protein expression showed all
subtypes of vasopressin receptors in this organ.
02.016 - THE ROLE OF THE RHOA PATHWAY IN NHE3 ACTIVITY DURING PERFUSION OF RAT
PROXIMAL TUBULES WITH SIMVASTATIN OR SILDENAFIL
PRISCILLA MARYS COSTA DOS SANTOS; THAISSA DANTAS PESSOA; GERHARD MALNIC
Sil is a drug that increases cGMP and inhibits RhoA, and this intracellular pathway has pronounced
inhibitory effects on NHE3. Simva modulates RhoA, and could alter the rate of endocytosis and
exocytosis of NHE3 increasing its expression in the apical membrane, and its activity. The aim of
this work was to determine the effects of Simva or Sil on NHE3 and to study intracellular signaling
pathways involved. By means of stationary microperfusion, PT of Wistar rats were perfused with
a 24mM HCO3- solution (CTRL) with or without 100µM Simva or 10µM Sil to determine bicarbonate
reabsorption (JHCO3-). Perfusion of Sil decreased JHCO3- by 20% and perfusion of Simva increased
JHCO3- by 19,31% compared to CTRL. To test if these effects were NHE3-dependent, PTs were
perfused with CTRL, Sil or Simva plus 2µM S3226, a specific NHE3 inhibitor. The reminiscent S3226insensitive JHCO3- was not different among groups, showing that NHE3 was modulated by Sil or
Simva. To determine if RhoA was involved in these effects, the PTs were perfused with the Rho
GTPase inhibitor Y-27632 (1µM). Perfusion of Y-27632 alone decreased JHCO3- by 20% compared
to CTRL. Moreover, the perfusion with Y-27632 and Sim reversed the stimulatory effect of Rho on
JHCO3- and increased the inhibitory effect of Sil by 26.16%. These data show that the increase of
JHCO3- by Simva was mediated by the RhoA pathway; and the decrease of JHCO3- by Sil was also
RhoA dependent.
THEME 03 - NEUROPHYSIOLOGY
03.021 - P2X PURINERGIC RECEPTOR BLOCKADE REDUCES NACL INTAKE IN FLUID DEPLETED
RATS TREATED WITH ALPHA2-ADRENERGIC AGONIST IN THE LATERAL PARABRACHIAL NUCLEUS
CAMILLA DIAS GUILLEN; GLAUCIA M. F. DE ANDRADE-FRANZÉ; PATRICIA MARIA DE PAULA; LAURIVAL
ANTONIO DE LUCA JR; JOSÉ VANDERLEI MENANI; CARINA AP. FABRÍCIO DE ANDRADE
DEPT. PHYSIOLOGY AND PATHOLOGY, SCHOOL OF DENTISTRY, FOAR - UNESP, ARARAQUARA, SP,
BRASIL.
Alpha2-adrenergic and P2X purinergic receptor activation in the lateral parabrachial nucleus
(LPBN) increases fluid depletion induced 0.3 M NaCl intake. In the present study, we investigated
0.3 M NaCl intake induced by fluid depletion in rats treated with bilateral injections of moxonidine
(alpha2-adrenoceptor/imidazoline receptor agonist) combined with P2X purinergic receptor
blockade in the LPBN. Male Holtzman rats (n = 5) with cannulas implanted bilaterally in the LPBN
were submitted to subcutaneous treatment with furosemide (FURO, 10 mg/kg) and captopril (CAP,
5 mg/kg) to induce sodium appetite. Bilateral LPBN injections of moxonidine (0.5 nmol/0.2 μl)
increased 0.3 M NaCl intake (27.9 ± 8.6, vs. vehicle: 2.7 ± 1.0 ml/120 min). The P2X purinergic
antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS, 2 nmol/0.2 μl) alone into
the LPBN did not change 0.3 M NaCl intake (2.9±1.1 ml/120 min). However, the pre-treatment
with PPADS into the LPBN abolished the effects of moxonidine on 0.3 M NaCl intake (8.5 ± 4.1
ml/120 min). These results suggest a possible interaction between alpha 2-adrenergic and P2X
purinergic receptors in the LPBN to control sodium intake.
Fnancial support: FAPESP, CNPq, PROPe-UNESP
03.022 - EFFECTS OF A GREEN TEA ON MEMORY AND OXIDATIVE BALANCE AFTER DIFFERENT
STROKE MODELS IN RATS
CAROLINE DALLA COLLETTA ALTERMANN; ALEXANDRE GARCIA DOS SANTOS; ALEXANDRE DOS
SANTOS MARTINS; HELEN LIDIANE SCHIMIDT; MAUREN ASSIS DE SOUZA; PAMELA BILLIG MELLO
CARPES
Stroke could be ischemic or hemorrhagic; both cause functional alterations and cognitive
impairment. Stroke also promotes reactive oxygen species (ROS) increase in brain. Here we
investigate the effect of green tea (GT) on memory and oxidative damage induced by transient
ischemia-reperfusion (IR) and intracerebral hemorrhage (IH). 80 Wistar male rats divided into 8
groups according the stroke type induced were used (n=10/group): Sham IR, Sham IR+GT, IR,
IR+GT, Sham IH, Sham IH+GT, IH, IH+GT. Supplementation with GT was initiated 10 days before
stroke surgery and continuous for 7 days after (GT dose 400 mg/kg). After all, the rats were
subjected to object recognition task (OR) to assess short (STM) and long term memory (LTM) and
hippocampus were used to measure parameters related to oxidative stress (ROS, lipid peroxidation
and total antioxidant capacity). OR results were analyses using one-sample t-test (theoretical
mean 50%) and biochemical parameters using one-way ANOVA and Dunn’s post hoc. IR and IH rats
showed STM and LTM deficits; GT intervention prevents STM and LTM deficits in both stroke models
(P=0.0005 STM IR; P=0.0021 STM IH; P<0.05 LTM IR; P=0.0006 LTM IH). IR and IH induces increase
in ROS concentration (P = 0.04 IR, P =0.006 IH) and lipid peroxidation (P=0.0006 IH) in the
hippocampus; GT supplementation avoids it. The oxidative imbalance and damage observed in IR
and IH rats could be related to the memory deficits. GT supplementation presented a
neuroprotective role.
03.023 - POSSIBLE INVOLVEMENT OF THE INHIBITORY MECHANISMS OF THE LATERAL
PARABRACHIAL NUCLEUS IN THE CONTROL OF SODIUM INTAKE IN RENOVASCULAR
HYPERTENSIVE RATS
RICHARD BOARATO DAVID1; CAMILA FERREIRA RONCARI1; MARIANA RUIZ LAUAR1; REGINA CELIA
VENDRAMINI2; DEBORA SIMÕES ALMEIDA COLOMBARI1; CARINA A.F. ANDRADE1; JOSÉ VANDERLEI
MENANI1; LAURIVAL ANTONIO DE LUCA JR 1
1.DEPTO. FISIOLOGIA E PATOLOGIA, PIPGCF UFSCAR-UNESP, FACULDADE DE ODONTOLOGIA,
UNESP, ARARAQUARA, SP, BRASIL; 2.DEPTO. ANÁLISES CLÍNICAS, FACULDADE DE CIÊNCIAS
FARMACÊUTICAS, UNESP, ARARAQUARA, SP, BRASIL.
Rats with 2 kidney, 1 clip (2K1C) renal hypertension have sustained increase in plasma renin
activity and arterial blood pressure from the 3rd up the 6th week after surgery. Daily 0.3 M NaCl
intake increases in these rats in the 3rd and 4th week post-surgery (~ 30 ml/day) and returns to
control levels in the 5th-6th week (~ 18 ml/day). Inhibitory mechanisms of angiotensin II-induced
sodium intake are present in the lateral parabrachial nucleus (LPBN) and may act to reduce sodium
intake chronically in 2K1C hypertensive rats. Thus, we tested 0.3 M NaCl intake in 2K1C
hypertensive rats with blockade of LPBN mechanisms by local activation of α2-adrenoceptors.
Male adult Holtzman rats (n = 4-7/group), with stenosis of the left renal artery (2K1C hypertensive)
and others with sham surgery, had daily access to both water and 0.3 M NaCl. Moxonidine (0.5
nmol/0.2 μl), an α2-adrenoceptor agonist, injected into the LPBN on the 7th week produced an
immediate and substantial 0.3 M NaCl intake in normohydrated 2K1C rats (20.5 ± 6.2, vs. vehicle:
0.6 ± 0.4 ml/2 h), but had no effect on sham rats (1.8 ± 1.1, vs. vehicle: 0.1 ± 0.1 ml/2 h). The
results suggest recruitment of LPBN inhibitory mechanisms to limit daily sodium intake after the
4th week of stenosis in 2K1C hypertensive rats.
03.024 - BEHAVIORAL AND HISTOCHEMICAL CHANGES INDUCED BY NA+/GLUCOSE
COTRANSPORTER 1 (SGLT1) INHIBITION IN MODEL OF TEMPORAL LOBE EPILEPSY
IGOR SANTANA DE MELO1; YNGRID MICKAELLI OLIVEIRA DOS SANTOS1; AMANDA LARISSA DIAS
PACHECO1; MAÍSA DE ARAÚJO COSTA1; NÍVEA KARLA DE GUSMÃO TAVEIROS SILVA 1; MARCELA
SAMPAIO DE ARAÚJO1; ROBINSON SABINO-SILVA2; OLAGIDE WAGNER DE CASTRO1
1.UNIVERSIDADE FEDERAL DE ALAGOAS, MACEIO, AL, BRASIL; 2.UNIVERSIDADE FEDERAL DE
UBERLÂNDIA, UBERLÂNDIA, AL, BRASIL.
We evaluated the functional role of Na+/glucose cotransporter 1 (SGLT1) in the behavior of limbic
seizures and neurodegeneration process. Experimental procedures were approved by the Ethical
Committee for Animal Research of UFAL (10/2014). Male Wistar rats (n=39 [240-340g]) were
submitted to stereotaxic surgery for implantation of a cannula in the hilus of dentate gyrus of
hippocampus. The animals received microinjections of saline (SAL, 1μL) or phlorizin (PZN,
50μg/μL) in the hippocampus followed 30 minutes later by pilocarpine (1.2mg/μL). Behavioral
analysis of seizures was performed for 90 minutes during of status epilepticus (SE), according to
Racine scale (1972). The animals were perfused after 24 hours or 15 days of SE and their brains
were collected and processed. Neurodegeneration was evaluated by Fluoro-Jade (FJ) and FJ
positive neurons (FJ+) were counted (ImageJ–NIH). Results were expressed as mean±SEM,
compared by unpaired t test. SGLT1 inhibition increased the severity of seizures. After 24 hours
of SE, PZN animals had a higher (p<0.05) number of FJ+ in the dentate gyrus (DG) (1205.0±116.9),
hilus (277.3±23.2), CA3 (338.0±36.8) and CA1 (400.2±60.5) of hippocampus when compared to the
same region of SAL group: DG (693.3±109.0), hilus (141.5±45.1), CA3 (170.2±61.1), CA1,
(221.0±52.7). However, 15 days after SE, PZN animals had a decreased number (p<0.05) of FJ+ in
CA1 (245.3±45.9) compared to the same region of SAL group (425.8±47.5). These data suggest that
possibly the SGLT1 participates in the modulation of epileptogenic processes.
03.025 - INTRACEREBROVENTRICULAR STREPTOZOTOCIN IMPAIRS SOCIAL BEHAVIOR IN WISTAR
RATS: IMPLICATIONS FOR ALZHEIMER'S DISEASE
ANNA CAROLINA PARRACHO MOTZKO SOARES; ROBSON CRISTIANO LILLO VIZIN; DANIEL MOREIRASILVA; MARIA CAMILA ALMEIDA; DANIEL CARNEIRO CARRETTIERO
UNIVERSIDADE FEDERAL DO ABC, SAO BERNARDO DO CAMPO, SP, BRASIL.
Streptozotocin (STZ), a chemical compound that promotes central insuline resistance, is used as
an animal model for sporadic Alzheimer’s disease (AD). In this study, we examine its influence in
social behavior in Wistar rats (20 weeks old) treated with intracerebroventricular (icv) STZ (4
mg/kg) or its vehicle. Memory impairment (using Y-maze test) and social behavior were analyzed
30 days later. In Y-maze test, exploration time in known arms [unblocked (UA); start arm (SA)]
and novel arm [blocked (BA)] was evaluated. In social behavior test, unknown rats were put in
pairs and time spent in social interaction was evaluated for 5 min. Values are expressed as mean
± SEM (n = 4). Interaction time is expressed in percentage. Y-maze test demonstrate STZ-treated
rats have no difference in time spent among arms (UA: 114.7 ± 17.5 s; BA: 69.3 ± 10.7 min; SA:
80.3 ± 23.1 min), while control group (CG) spent less time in the UA (45.7 ± 4.5 s) as compared to
BA (87.3 ± 5.2 s) and to SA (95.3 ± 7.1 s). Furthermore, STZ-treated animals spent more time
20
within UA as compared to CG. In social behavior test, STZ–treated animals (53.7 ± 2) interact less
than CG (65.7 ± 0.7) - p < 0.05 for all tests. The relationship between STZ animal model and social
behavior test was not well established in the literature. Our data show, for the first time, icv STZ
impairs social behavior in Wistar rats. The result is a new piece in the complex puzzle of sporadic
animal model for AD.
03.026 - ESTRADIOL ATTENUATES HYPERPHAGIA CAUSED BY THE ACTIVATION OF 5-HT1A
RECEPTORS IN THE LATERAL HYPOTHALAMIC AREA IN SATIATED RATS
ANA PAULA DAMBROS TASCHETTO; LARISSA KOCHENBORGER; BRUNNO ROCHA LEVONE; RAFAEL
APPEL FLORES; MOACIR SERRALVO FARIA; MARTA APARECIDA PASCHOALINI
UFSC, FLORIANOPOLIS, SC, BRASIL.
The aim of the study was evaluate the ingestive and not ingestive effects caused by injection into
the lateral hypothalamic area (LHA) of the 8-OH-DPAT (receptor agonist 5-HT1A e 5-HT7, 6nmol),
WAY 100635 (selective receptor antagonist 5-HT1A, 0,37 nmol) and MM77 (receptor antagonista
5-HT1A, 0,37 nmol) in ovariectomized rats (OVX) and ovariectomized treated with estradiol (OVX
+ E). Injections of 8-OH-DPAT in OVX rats significantly increase food intake duration and the
frequency of this behaviour. When animals are treated with estradiol (E2), we did not observe a
significant difference in these behaviours. The latency to food intake did not change with the
different treatments in OVX or in OVX + E. When animals were pre-treated with the antagonists
WAY10063 and MM 77, the hyperphagic effects evoked by 8-OH-DPAT were attenuate, as well the
increase in the duration and frequency of this behaviour in untreated OVX rats. The another non
ingestive behaviours (locomotion, immobility, non locomotor exploration, rearing and grooming)
did not change in OVX or OVX + E. Water intake also remains unchanged in OVX or OVX + E,
excluding a possible involvement of 5-HT1A receptors, present in the AHL, in the control of water
intake. These results confirm the involvement of 5-HT1A receptors present in the AHL in the
control of food intake suggesting that ovarian steroids interact with the serotoninergic system
present in the AHL, attenuating the ingestive response after 5-HT1A receptors activation.
03.027 - AN OVERVIEW OF THE WISTAR AUDIOGENIC RAT (WAR) STRAIN CONTRIBUTIONS TO
PHYSIOLOGY AND NEUROSCIENCE: CHALLENGES AND OPPORTUNITIES
NORBERTO GARCIA-CAIRASCO; EDUARDO UMEOKA
FMRP-USP, RIBEIRAO PRETO, SP, BRASIL; .
Epilepsy comorbidities, bring a new potential to the Wistar Audiogenic Rat (WAR) a geneticallyselected strain of rats suitable for epileptology, to be studied in neuropsychiatry, aging,
endocrinology and cardio-respiratory regulation. At the Physiology Department of the FMRP-USP
in 25 years we have produced over 53 generations of inbreeding: 7.320 WAR animals being in the
last five years: ♂ 689, ♀ 673 (Total 1.362).
WARs are endogenously anxious with reduced exploration in the elevated plus maze/open field
and are smaller than Wistars, with hyperplasic adrenal gland/higher levels of corticosterone, after
exogenous ACTH injection. Naïve WARs display higher grooming behavior when compared to
Wistars microinjected with oxytocin into the amygdala, so they have potential for studies of
compulsive behavior. These alterations are additional to higher blood pressure and altered
autonomic regulation of the cardiovascular system and highly compromised brainstem 5-HT nuclei
associated to respiratory control. The Morris Water Maze revealed impaired reference memory in
young WARs and the Novel Object Recognition test indicated deficit in short-term memory in
middle age WARs. The additional detection of altered β- amyloid protein and phosphorylated-Tau
in the brain of WARs encourage the use of WARs as a model of Epilepsy-Alzheimer disease
comorbidities.
WARs are then a suitable model to study the complexity of normal brain function, as well as
neuropsychiatric diseases, then we are donating WAR embryos to be available through the Rat
Research ad Resource Center (RRRC) in Missouri, USA.
03.028 - EFFECT OF FLUOXETINE ON INHIBITORY AVOIDANCE MEMORY IN ASTYANAX SP
ANA PAULA FERREIRA; ANA AMÉLIA MAIA SILVA; LILIAM MIDORI IDE
UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI, SAO JOAO DEL REI, MG, BRASIL.
INTRODUCTION. The inhibitory avoidance involves the formation of a declarative memory in which
the animal learns to inhibit an innate response to avoid an aversive stimulus. We investigated the
effect of fluoxetine, a selective 5-HT reuptake inhibitor (SSRI), in learning and memory processes,
in an inhibitory avoidance test in Astyanax sp. METHODS. The experimental apparatus consisted
of a rectangular aquarium divided into two compartments (black and white), with a central door.
After the fish crossed the line between compartments a 45 g weight was dropped in the black
side. During training, this procedure was done seven times in a row. The animals were not treated
or submitted to a single i.p. injection of saline (0.01 ml/g b.w.) or fluoxetine hydrochloride (10
or 100 ug/g b.w), 30 min before training. After that, the retention tests were performed 3 and 24
h after training. Latency to enter the black compartment was assessed and higher latencies were
indicative of increased avoidance learning. RESULTS. The control animals showed an increase in
the average of six training latencies, indicating learning (Friedman, followed by Dunn post-hoc
comparison, P≤0.001). The animals treated with 100 ug/g b.w. fluoxetine showed a significant
increase in the latencies in the average latencies of 6 tests, 3 and 24 h after training, indicating
retention (Kruskal-Wallis, followed by Dunn's Test; P=0.022; P=0.05; P=0.005). CONCLUSIONS.
These results show that fluoxetine acts influencing the retention of inhibitory avoidance memory
and cause an anxiolytic-like effect in Astyanax sp.
03.029 - INHIBITORY AVOIDANCE LEARNING AND ARGYROPHILIC NUCLEOLAR ORGANIZER
REGION OF DORSOMEDIAL TELENCEPHALIC NEURONS IN LEPORINUS MACROCEPHALUS
ANA AMÉLIA MAIA SILVA; ANA PAULA FERREIRA; LILIAM MIDORI IDE
UNIVERSIDADE FEDERAL DE SÃO JOÃO DEL-REI, SAO JOAO DEL REI, MG, BRASIL.
INTRODUCTION. The cellular activity related to transcriptional activity may be evaluated by
measuring the argyrophilic nucleolar organizer region (AgNOR). In this study we used the AgNOR
neurohistochemical stain technique to assess morphological changes in dorsomedial telencephalic
neurons in Leporinus macrocephalus submitted to inhibitory avoidance training. METHODS. The
inhibitory avoidance apparatus consisted of a rectangular aquarium divided into two
compartments (black and white), with a central door. After the fish crossed the line between
compartments a 45 g weight was dropped in the black side. During training, this procedure was
done seven times in a row. The behavioral retention tests were performed 3 and 15 days after
training (n=10), and trained animals (n=8) or submitted to a similar non-contingent behavioral
procedure (control group, n=8) were morphologically evaluated. The brain sections were observed
under an optical microscope at 1000x magnification and the images captured and analyzed using
ImageJ software. RESULTS. The animals showed an increase in the average of six training
latencies, indicating learning (Friedman, followed by Dunn post-hoc comparison, P≤0.001). The
number and the relative area of AgNOR in the dorsomedial telencephalic neurons analyzed in the
five trained animals and three in the control group were statistically similar. CONCLUSIONS. These
partial results allow to conclude that L. macrocephalus tested do not show specific neuronal
changes after undergoing inhibitory avoidance task.
03.030 - SEROTONERGIC ACTIVATION IN THE NTS INHIBITS WATER INTAKE INDUCED BY CENTRAL
ANGIOTENSIN II
RODRIGO ANDERSON TOMEO; JOSÉ VANDERLEI MENANI; PATRICIA MARIA DE PAULA
UNIVERSIDADE ESTADUAL PAULISTA, ARARAQUARA, SP, BRASIL.
The nucleus of the solitary tract (NTS), the primary site of peripheral osmoreceptor and
cardiovascular afferences, is suggested to receive important inhibitory signals involved in the
control of water and sodium intake, however, the neurotransmitters involved in this mechanism
are not known. Central serotonergic mechanisms are involved in the control of sodium and water
intake and immunohistochemical studies provided evidence for serotonergic receptors in the NTS.
Therefore, in the present study, we investigated the effects of DOI (a serotonergic 5-HT2A/2C
receptor agonist) injected into the NTS on water intake induced by intracerebroventricular (icv)
angiotensin II (ANG II). Male Holtzman rats (290-310 g, n=11) with bilateral stainless steel cannulas
implanted into the NTS and into the lateral ventricle (LV) were used. Thirsty was induced by icv
ANG II (50 ng/1 µl) administered 45 minutes before bilateral injections of DOI (2.5 µg/100 nl) or
vehicle into the NTS (CEUA FOAR 14/2013). Bilateral injections of the serotonergic 5-HT2A/2C
receptor agonist DOI into the NTS reduced water intake induced by icv ANG II in the first 15
minutes of water access (9.9 ± 1.1, vs. saline 14.5 ± 1.5 ml, p<0.05). The present results suggest
that serotonin in the NTS may activate inhibitory mechanisms that participate in the control of
water intake induced by central ANG II.
03.031 - ISCHEMIC STROKE DECREASES SERUM ACTIVITY OF ANGIOTENSIN CONVERTING ENZYME
2 IN CORRELATION WITH SYSTOLIC BLOOD PRESSURE IN HUMANS
DOUGLAS M BENNION; CHRISTIAN ROSADO; EMILY A HALTIGAN; MICHAEL F WATERS; COLIN
SUMNERS
UNIVERSITY OF FLORIDA, GAINESVILLE FLORIDA, ESTADOS UNIDOS.
Extensive preclinical data suggest that the angiotensin converting enzyme 2/angiotensin-(1-7)
[ACE2/Ang-(1-7)] axis is a promising target for inducing stroke neuroprotection. Ischemic stroke
in rats results in initially decreased serum ACE2 activity with rebound increases by 3 days. Here,
we assessed the effects of ischemic stroke on endogenous ACE2 activity in human serum following
ischemic stroke (n=17) or in non-stroke controls (n=15). We also performed regression analyses to
correlate levels of circulating ACE2 activity with systolic blood pressure (SBP) during stroke and
among healthy adults (n=16). Ischemic stroke resulted in a significant decrease in serum ACE2
activity at an average of 3.5 hours after symptom onset as compared to non-stroke controls
(p<0.005), followed by a rebound return in activity to control levels at 3 days post stroke. Among
acute stroke patients, serum ACE2 activity was significantly negatively correlated with SBP
(p=0.006, Pearson r = -0.59). This was reversed among healthy adults where plasma ACE2 activity
was positively correlated with SBP (p=0.023, Pearson r = 0.56). Among stroke and non-stroke
controls, those with serum ACE2 activity levels in the highest tertile had 10.5 times greater odds
(95% CI: 1.36–81.052) of having suffered an ischemic stroke than those in the lowest tertile. These
results provide novel insight into dynamic alterations of the protective renin angiotensin system
in human ischemic stroke, and the possible role of acute changes in blood pressure on serum ACE2
activity levels.
03.032 - MENTHOL-INDUCED HYPERTHERMIA IS PARTIALLY ATTENUATED AFTER REPEATED
DAILY ADMINISTRATION IN RATS
ROBSON CRISTIANO LILLO VIZIN; DÉBORA ISHIKAWA; DANIEL CARNEIRO CARRETTIERO; MARIA
CAMILA ALMEIDA
UNIVERSIDADE FEDERAL DO ABC, SAO BERNARDO DO CAMPO, SP, BRASIL.
Menthol is an organic compound known for its cooling effects by acting as a TRPM8 channel
agonist. TRPM8 channel is also stimulated by temperatures below 25°C and activates autonomic
and behavioral cold-defense responses. In fact, menthol-treated animals activate heat-gain
mechanisms, developing hyperthermia. However, the effects of chronic treatment with menthol
on rat thermoregulation are not clear. In this study, we investigated the influence of daily menthol
treatment on body temperature (Tb). Male Wistar rats (9 weeks old; weight ~310 g; n = 6 per
group; ethics approval: CEUA/UFABC protocol n° 20/2014) were treated daily for 6 days with 3
mL of menthol 5% or its vehicle (100% propyleneglycol) applied topically and maintained in
individual cages at 26°C of ambient temperature throughout the experiment. T b was recorded by
miniature temperature data logger (SubCue) implanted into the peritoneal cavity via midline
laparotomy. Our data revealed that menthol promotes significant increase in T b at 30 - 50 min
after treatment compared to the control group on all days of the study. However, the
hyperthermia in the 1° day (0.8 ± 0.05°C) was significantly reduced from days 3–6 (0.4 ± 0.08°C;
0.5 ± 0.08°C; 0.3 ± 0.05°C; 0.4 ± 0.07°C; 0.3 ± 0.04°C, respectively; p < 0.05). In addition, the
duration of hyperthermia decreased from 11 h in the first day to 6.5 h in the last day of treatment.
We speculate that reduction in hyperthermia by chronic treatment with menthol was likely due
to a reduction in the TRPM8 channel activity.
03.033 - EFFECTS OF STRESS AND RELATED HORMONES ON FEEDING, ENDOCRINE, METABOLIC
AND ANXIETY-LIKE BEHAVIORS
DANIELA ORTOLANI1; GARCIA M C2; MELO-THOMAS L2; TECOTT L H3; SPADARI R C2
1.UNIFESP, SANTOS, SP, BRASIL; 2.DEPARTMENT OF BIOSCIENCES, FEDERAL UNIVERSITY OF SÃO
PAULO, CAMPOS, SP, BRASIL; 3.DEPARTMENT OF PSYCHIATRIC, UNIVERSITY OF CALIFORNIA, SAN
FRANCISCO, ESTADOS UNIDOS.
This work investigated the alterations on feeding, endocrine, metabolic and anxiety-like behaviors
in rats submitted to one daily session of footshock stress (FS), 3 days or to 14 days of chronic
unpredictable mild stress (CUMS), and in mice treated during 14 days with corticosterone (CORT,
100 ug/ml) in the drinking water. FS and CUMS decreased food intake and body weight. The
opposite effects were observed in CORT mice. FS increased concentrations of insulin and glucose
and, decreased triglycerides. In contrast, CUMS did not alter any metabolic parameter. Both
stressors increased concentrations of corticosterone. CORT treatment increased concentrations
of insulin, leptina, triglycerides and corticosterone, in contrast, decreased ghrelin. FS decreased
anxiety-like behavior in the elevated plus maze and open field tests. The opposite was observed
in CUMS. Locomotor patterns were not altered in both types of stressors. In contrast, the CORT
treated mice were showed anxiety-like behaviors in the elevated zero maze (EZM), but they
decreased the time of exploration of novel object. Locomotor behavior was decreased by CORT
treatment in the EZM test. These data indicate that different types of stressors may induce
alterations on feeding, endocrine and metabolic parameters as well as anxiety-like behaviors in a
specific way. Moreover, despite the CORT treatment did not reproduce the stress effects it may
be a great model to study the metabolic syndrome and obesity.
03.034 - INCREASED LEVELS OF SERUM VASOPRESSIN EVOKED BY CHOLINERGIC ACTIVATION IN
THE FOURTH BRAIN VENTRICLE (4THV) IN FEMALE WISTAR RATS
EDUARDO MAZUCO CAFARCHIO1; ROBERTO LOPES DE ALMEIDA1; CRISTIANA AKEMI OGIHARA1;
LEANDRO DE CARVALHO VALDO1; MARIA CRISTINA1; FERNANDO LUIZ AFFONSO FONSECA1; JANAÍNA
SENA DE SOUZA2; RUI MONTEIRO DE BARROS MACIEL2; GISELE GIANNOCCO2; MONICA AKEMI SATO1
1.FMABC, SANTO ANDRE, SP, BRASIL; 2.UNIFESP, SAO PAULO, SP, BRASIL.
Aim: We investigated if carbachol injection into the 4th V induces vasopressin (AVP) or oxytocin
(OT) release and the existence of AVP and OT receptors in the urinary bladder (UB).
Methods: Female Wistar rats (~250 g, N= 6/group) implanted with guide cannulas into the 4th V
five days prior to the experiments were anesthetized with 2% isoflurane in 100% O2, and carbachol
(4 nmol/µL) or saline (1 µL) was injected into the 4th V. Thirty minutes after injection, a blood
sample was collected and plasma AVP and OT was determined by ELISA kit (Cayman
Chemical).Data are as mean±SE and were submitted to Student t-test (p<0.05).
Results: Carbachol induced plasma AVP release (3051±85 pg/mL) compared to saline into the 4th
V (2628±276 pg/mL). No difference was observed in plasma OT in rats which received carbachol
(532±33 pg/mL) or saline (508±38 pg/mL).
Conclusion: Cholinergic activation of brain stem neurons by carbachol induces plasma AVP release.
The increase in IP evoked by carbachol is likely due to AVP release which binds to AVP receptors
in the urinary bladder.
03.035 - DIFFERENT DOSES OF ESTRADIOL MODULATE HIDROELECTROLYTE STATUS UNDER
BASAL AND SODIUM DEPLETION CONDITION
VERONICA CRISTINA LOPES MENEZES; RAONI DA CONCEIÇÃO DOS SANTOS; BRUNO PAES LEME
FERREIRA; LÍVIA DA ROCHA NATALINO MONTEIRO; ANDRÉ DE SOUZA MECAWI; LUIS CARLOS REIS
FEDERAL RURAL UNIVERSITY OF RIO DE JANEIRO, SEROPÉDICA, RJ, BRASIL.
Estrogen receptors are expressed in important brain structures that regulate hydroelectrolytic
balance. We verified if estrogen replacement can modulate in a dose-response manner
hydroelectrolytic status of female ovariectomized (OVX) rats. OVX Wistar rats (~230 g) were
adapted to metabolic cages. Experimental groups were: OVX, EC 2.5, EC 10 and EC 40 µg/kg.
(n=16, each group). We injected estradiol cypionate (EC, Pfizer, s.c. daily during 7 days).OVX
group received daily injections of corn oil. We evaluated daily water, hypertonic saline (1.8%) and
food intake under basal conditions. Sodium depletion was done in 7th day (furosemide 20 mg/kg,
s.c.) and low sodium diet and cumulative water and saline intake were measured until 300 min.
Uterine and pituitary were weighted to verify surgery efficacy. Data was analyzed by two-way
Anova repeated measures and Bonferroni post test and expressed by means±SEM. Project was
21
approved by the Ethical Committee in UFRRJ (nº 23083.011699/2013-99). In basal conditions water
and saline intake weren’t altered by estrogen status, but food intake was reduced in OVX vs. EC
10 (8.1±0.2 vs. 7.2±0.3 g/100 g, p<0.05) and OVX vs. EC 40 (8.1±0.2 vs. 6.8±0.2 g/100 g, p<0.001)
in the 6th day. After sodium depletion cumulative water intake was increased between EC 40 vs.
OVX (1.1±0.2 vs. 0.4±0.2 ml/100 g, p<0.001) at 60 min of evaluation and hypertonic saline intake
was increased between EC 2.5 vs. OVX (5.0±0.4 vs. 3.6±0.7 ml/100 g, p<0.001) at 240 min.
Genomic mechanisms could be probably placed at midbrain structures implied with hidromineral
balance.
03.036 - CO HAS DISTINCT EFFECTS ON SON MAGNOCELULAR NEURON BASAL ACTIVITY
JULIANA BEZERRA MEDEIROS DE LIMA; MELINA PIRES DA SILVA; JOSÉ ANTUNES-RODRIGUES;
WAMBERTO ANTONIO VARANDA
USP, RIBEIRAO PRETO, SP, BRASIL.
A novel class of neuromodulators has been recently described in the literature, the gaseous
molecules, among them carbon monoxide (CO). The presence of CO has been shown in brain areas
involved in the neuroendocrine control of hydroelectrolytic balance such as supraoptic nucleus. It
has been shown that CO could modulate the hypothalamus-neurohypophysial axis. In this context,
the present study aims to evaluate if the CO system affects the basal electrical activity of SON
magnocellular neurons and also if there is a phenotype-dependent action. For this purpose, 80100g male Wistar rats were decapitated, their SON were sectioned into 250-µm slices and used
for whole-cell patch clamp recordings. Two different patterns of SON magnocellular spontaneous
activity were observed in response to 30-µM ZnDPBG (hemeoxigenase inhibitor); one in which cells
presented an increase in the spontaneous firing rate F 2,21 = 6.394, p<.0001) when exposed for 5
(control 4.98 ± 1.15; ZnDPBG 5 min 5.69 ± 1.23, p<.01) or 10 (control 4.98 ± 1.15; ZnDPBG 10 min
6.08 ± 1.32, p<.001) minutes to ZnDPBG and another in which the cells had a decrease in their
basal activity (F2,23 = 6.459, p<.0001) when exposed for 5 (control 5.84 ± 0.57; ZnDPBG 5 min 4.61
± 0.49, p<.01) or 10 (control 5.84 ± 0.57; ZnDPBG 10 min 4.07 ± 0.65, p<.001) minutes to ZnDPBG.
These results suggest that CO modulates SON magnocellular basal activity; although, how it acts
in two distinct ways remains to be determined.
THEME 04 - CARDIOVASCULAR PHYSIOLOGY
04.037 - MECHANISMS INVOLVED IN EXERCISE-INDUCED IMPROVEMENT IN ENDOTHELIAL
DYSFUNCTION IN LDL RECEPTOR-DEFICIENT MICE DURING EARLY STAGE ATHEROSCLEROSIS
DANIELE MENDES GUIZONI; GABRIEL DE GABRIEL TAFFARELLO DORIGHELLO; AMANDA CHRISTINE
SPONTON; HELENA C.F. OLIVEIRA; MARIA ANDRÉIA DELBIN; MARTA HELENA KRIEGER; ANA PAULA
DAVEL
UNICAMP, CAMPINAS, SP, BRASIL.
The aim of this study was to elucidate the effect of exercise training (Ex) on the endothelial
dysfunction occurring in the early stages of atherosclerosis in LDL-receptor knockout mice (KO).
Twelve-week-old C57BL/6J (WT) and KO mice were divided into sedentary (S) or 4-week
exercised-groups (Ex) (treadmill; 1h/day, 5days/week). KO-S and KO-Ex exhibited similarly
elevated plasma levels of cholesterol and triacylglycerol. The acetylcholine (ACh)-induced
relaxation was impaired in aorta of KO-S compared with WT-S; this difference was abolished by
the nitric oxide (NO) synthase (NOS) inhibitor L-NAME, or by the NADPH oxidase inhibitor apocynin.
NO bioavailability and protein expression of p-eNOS(Ser1177) and SOD isoforms were reduced in
the aorta of KO-S. Nox2 subunit of NADPH oxidase and reactive oxygen species (ROS) generation
were increased in aortas of KO-S. KO-Ex mice displayed reduced Nox2-derived ROS production and
normal ACh-induced relaxation and p-eNOS(Ser1177), SOD-1 and -3 protein expression. There was
no detectable fat deposition in thoracic aorta in the studied groups. Moderate atherosclerotic
plaques found in the aortic sinus of KO-S mice were not modified by Ex. In conclusion, Ex is a
potential early therapy for the prevention of endothelial dysfunction and oxidative stress prior
the development of atherosclerotic lesions and independent of serum cholesterol levels.
04.038 - PREVIOUS EXPOSURE TO CHRONIC INTERMITTENT HYPOXIA ATTENUATES THE
DEVELOPMENT OF RENAL HYPERTENSION (1K, 1C) IN RATS
RAPHAEL RODRIGUES PERIM; LENI GH BONAGAMBA; MATEUS RAMOS AMORIM; BENEDITO HONORIO
MACHADO
FACULDADE DE MEDICINA DE RIBEIRÃO PRETO, USP, RIBEIRAO PRETO, SP, BRASIL.
Chronic intermittent hypoxia (CIH) leads to hypertension, due to respiratory-related excitation of
RVLM pre-sympathetic neurons. The hypertension model 1K,1C is also partially dependent on the
sympathetic overactivity. We hypothesized that CIH preconditioning would facilitate the
development of 1K, 1C hypertension. Male rats were divided into 4 groups: Con-1K,1C, maintained
under normoxia during 25 days, followed by 1K,1C (n=19); Con-Sham, maintained under normoxia
during 25 days, followed by Sham surgery (n=19); CIH-1K,1C, exposed to CIH and then to 15 days
under normoxia, followed by 1K,1C (n=19); CIH-Sham, exposed to CIH and then to 15 days under
normoxia, followed by Sham surgery (n=18). Cardiovascular recordings were performed 9 days
after surgery. Baseline mean arterial pressure (MAP) was: a) higher in Con-1K,1C than in Con-Sham
rats (140±6 vs. 96±1 mmHg; p<0.05); b) higher in CIH-1K,1C than CIH-Sham rats (117±3 vs. 93±1
mmHg; p<0.05); c) higher in Con-1K,1C than in CIH-1K,1C rats ( p<0.05). Hexamethonium-induced
fall in MAP was reduced in CIH-1K,1C compared to Con-1K, 1C rats (-52±3 vs. -62±3 mmHg; p<0.05).
Chronic renal denervation precluded 1K,1C-hypertension, abolishing the preconditioning effects
of CIH. Additionally, removal of the carotid body eliminated CIH preconditioning. In conclusion,
CIH preconditioning attenuated the development of 1K,1C hypertension by reduction in the
sympathetic outflow.
04.039 - CHRONIC ETHANOL CONSUMPTION INDUCED VASCULAR OXIDATIVE STRESS AND
HYPERTENSION: ROLE OF β1-ADRENERGIC RECEPTORS
GABRIEL TAVARES VALE; CARLOS RENATO TIRAPELLI
Chronic ethanol consumption induces hypertension and oxidative stress. This study evaluated the
role of β1-adrenergic receptor in those responses. Male Wistar rats were divided into 4 groups of
5-weeks treatment: control (C), ethanol 20% (v/v) (E), control+nebivolol (10mg/kg/day, p.o.) (CN)
and ethanol+nebivolol (EN). (CEUA Protocol: 14.1.357.53.2). One-way ANOVA followed by Newman
Keuls test (p<0.05). Ethanol increased systolic blood pressure (mmHg) that was prevented by
nebivolol (C: 118±1.2, n=12; E: 138±1.9*, n=12; CN: 117±1.1, n=12; EN: 119±1.1, n=12). Ethanol
increased plasma (nmol/ml) and aortic (nmol/mg protein) TBARS levels that were prevented by
nebivolol (Plasma: C: 10.4±1.3, n=9; E: 20.6±4.2*, n=8; CN: 10.5±1.3, n=12; CN: 9.8±1.3, n=10);
(Aorta: C: 31.5±3.5, n=7; E: 54.1±5.1*, n=6; CN: 28.5±3.3, n=11; EN: 32.3±4.5, n=10). No
differences were found in plasma 8-isoprostane and Nitrate/nitrite (NOx) levels. But in aorta
ethanol reduced NOx levels (μg/mg protein), that was prevented by nebivolol (C: 18.5±2.1, n=8;
E: 5.6±0.8*, n=5; CN: 13.7±2.9, n=7; EN: 15.5±2.9, n=7). Nebivolol and ethanol+nebivolol reduced
aortic SOD activity (% inhibition rate) (C: 94.2±12.5, n=6; E: 96.7±5.9, n=6; CN: 48.8±6.2*, n=6;
EN: 44.4±5.7*, n=7). No difference was found in catalase activity (plasma and aorta) and in aorta
GSH levels. In plasma nebivolol and ethanol+nebivolol altered GSH levels (µg/ml) (C: 3.8±0.6, n=9;
E: 4.3±0.8, n=8; CN: 7.2±0.8*, n=10; EN: 1.3±0.2*, n=10). Chronic ethanol consumption-induced
hypertension and oxidative stress is mediated by β1-adrenergic receptor.
04.040 - CARDIOVASCULAR MODULATION AFTER TREADMILL TRAINING AND NNOS INVOLVEMENT
IN THE PARAVENTRICULAR NUCLEUS OF HYPOTHALAMUS (PVN)
NATHALIA ZERBINATTI FERREIRA1; HIVINY DE ATAIDES RAQUEL 1; LISETE COMPAGNO MICHELINI2;
MARLI CARDOSO MARTINS-PINGE1
1.UEL, LONDRINA, PR, BRASIL; 2.USP, SÃO PAULO, SP, BRASIL.
The PVN plays an important role in the modulation of cardiovascular responses. Also, physical
training can promote significant changes in nitric oxide (NO) in this nucleus. Thus, the aim of this
study was to evaluate the cardiovascular parameters after nNOS inhibition in the PVN and analyze
nNOS gene expression in sedentary (Gsed) and trained (Gtr) animals. Wistar rats were subjected
to eight weeks of inactivity or training on a treadmill. The animals were submitted to stereotactic
surgery for implantation of guide cannulas to PVN, and artery and vein catheters for mean arterial
pressure (MAP) and heart rate (HR) records. Bilateral microinjection of nNOS (n-Propyl-L-Arginine)
was made in the PVN. nNOS gene expression in PVN was analyzed by Real Time qPCR. The results
showed bradycardia at rest for trained animals (Gsed: 356±8 vs Gtr: 313±6 bpm) without changes
in MAP. The nNOS inhibitor microinjected in PVN caused greater increase of MAP (Gsed: 24±1 vs.
Gtr: 31±2mmHg) and HR (Gsed: 135±4 vs. Gtr: 156±5 bpm) in trained rats. Furthermore, the
training induced higher nNOS gene expression in PVN (Gsed: 1.6±0.2 vs. Gtr: 2.8±0.6). Our data
indicate that treadmill training modulates the cardiovascular parameters in part due to the
increase of nNOS in the PVN.
04.041 - ALAMANDINE SIGNALING IN CARDIOMYOCYTES IN HEALTHY AND DISEASE
ITAMAR COUTO GUEDES DE JESUS; SÉRGIO RICARDO ALUOTTO SCALZO JÚNIOR; CIBELE ROCHA
RESENDE; THAIS CRISTINA ALCÂNTARA; ROBSON AUGUSTO SOUZA DOS SANTOS; SILVIA CAROLINA
GUATIMOSIM FONSECA
Background: Almandine is a new component of the renin-angiotesin system. Its biological actions
include vasodilation, and antihypertensive effect in SHR rats. In the heart, the signaling pathways
activated by alamandine have not been characterized. Methodology: Ventricular myocytes were
isolated from C57BL/6 mice and rats that overexpress renin (TGR (mRen) 27). Additionally,
cardiomyocyte culture was prepared from 3 day-old neonatal rats. Cardiac cells were treated
with 100 nM alamandine and/or angiotensin II at the same concentration. Intracellular nitric oxide
and Ca2+ levels were assessed by DAF-FM and Fluo4-AM, respectively, by confocal microscopy.
Results: In ventricular myoctes alamandine treatment for 15 minutes resulted in increased
phosphorylation of PDK1 targets, and Akt. Importantly, alamandine induced Akt phosphorylation
was preserved in cardiomyocytes from Mas-KO mice. Moreover, alamandine induced an increase
in NO levels, without altering Ca2+ levels. Ventricular myocytes treated with alamandine also
showed decreased phosphorylation of GSK3β, and increased phosphorylation of ERK1/2. Opposite
results were obtained when mRen cardiomyocytes were exposed to alamandine. Alamandine
increased GSK3β phosphorylation and decreased phosphorylation of ERK1/2. The effect of
alamandine in Akt phosphorylation levels was preserved in mRen cells. Neonatal rat
cardiomyocytes treated for 36 hours with alamandine showed increased NO production.
Furthermore, alamandine treatment prevented both cardiomyocyte hypertrophy and nuclear
translocation of GRK5 induced by angiotensin II.
04.042 - ROLE OF AEROBIC EXERCISE TRAINING IN THE CARDIOVASCULAR AUTONOMIC
FUNCTION IN AN EXPERIMENTAL MODEL OF SUBCLINICAL METABOLIC SYNDROME
ERIKA ALVARENGA CORRÊA GOMES; ALESSANDRA CHOQUETA DE TOLEDO ARRUDA; RENATA
FRAUCHES MEDEIROS; PEDRO PAULO DA SILVA SOARES; ANTONIO CLAUDIO LUCAS DA NÓBREGA
High fructose consumption contributes to metabolic syndrome incidence, whereas aerobic
exercise training is an important tool in its prevention and treatment. The aim of the present
study was to investigate the effects of exercise training on arterial pressure (AP), heart rate
variability (HRV), and baroreflex sensitivity (BRS) in an experimental model of subclinical
metabolic syndrome in rats. Male Wistar rats (n=7/group), adults, were divided into four groups:
Control group (C; drinking water), Fructose group (F; water with 10% fructose), both for 10 weeks;
Exercise group (E) and Fructose+Exercise group (FE), by adding aerobic training on a treadmill in
the last 8 weeks. After the end of experimental protocol catheters were fitted into the rats'left
femoral artery for blood pressure and heart rate measurements during 24 hours. HRV parameters
were calculated in time and frequency domain. There were no differences in body mass gain,
caloric intake, glucose and blood pressure among groups. Exercise reduced hyperinsulinemia and
hypertriglyceridemia in the Fructose group. Despite increasing physical capacity in the fructose
group (p<0.05 E and FE vs C and F) exercise was not able to reduce the impairment in the
spontaneous baroreflex sensitivity in this group (p<0.05). Exercise training increased autonomic
nervous activity in the time domain (rMSSD and SDNN) compared to the other groups (p=0.01). In
conclusion, exercise training was not able to improve spontaneous baroreflex sensitivity in an
experimental model of subclinical metabolic syndrome in rats.
04.043 - ACUTE HYPOXIA INCREASES THE INTRINSIC EXCITABILITY OF RVLM PRESYMPATHETIC
NEURONS IN JUVENILE RATS EXPOSED TO CHRONIC INTERMITTENT HYPOXIA
MARLUSA KARLEN-AMARANTE; CARLOS EL ALMADO; DANIELA ACCORSI-MENDONÇA; DAVI JA
MORAES; BENEDITO HONORIO MACHADO
In this study we evaluated the effects of acute hypoxia (AH) on the intrinsic electrophysiological
properties of RVLM presympathetic neurons of juvenile rats exposed to chronic intermittent
hypoxia (CIH). To label the bulbospinal RVLM presympathetic neurons, bilateral microinjections
of a fluorescent retrograde tracer (rhodamine retrobeads) were performed into the
intermediolateral column of spinal cord (T3-T5) of anesthetized male Wistar rats (P-21). Two days
later, rats enter into the protocol of CIH or normoxic condition for 10 days. On the 11th day, under
anesthesia, brainstem slices were obtained and only the labeled RVLM presympathetic neurons
were recorded using whole-cell patch-clamp. The intrinsic electrophysiological properties were
analyzed before and after AH, which was produced by slice perfusion with hypoxic solution (95%
N2 and 5% CO2) during 2 min in the presence of fast synaptic blockers. All recorded RVLM
presympathetic neurons presented intrinsic pacemaker activity and the baseline firing frequency
of these neurons from normoxic and CIH group were similar. In the normoxic group, AH produced
a decrease in the intrinsic firing frequency (5.77 ± 0.79 vs 4.94 ± 0.44 Hz; n=32), while in CIH
group AH produced a significant increase in the intrinsic firing frequency (6.27 ± 0.86 vs 8.56 ±
1.12 Hz; n=20). These data show that the AH decreased the intrinsic firing frequency of RVLM
presympathetic neurons in normoxic group, while CIH preconditioning induced an increase in the
intrinsic excitability of RVLM presympathetic neurons in response to AH.
04.044 - TRATAMENTO COM ESCITALOPRAM CAUSA ALTERAÇÔES CARDIOVASCULARES EM
RATOS ADULTOS
LUIZ FERNANDO VERISSIMO1; VINICIUS LUCCA VOLPINI1; NATÁLIA KIMIE MATSUBARA1; DENIS CARLOS
DOS SANTOS1; VIVIANE BATISTA ESTRADA1; GISLAINE GARCIA PELOSI1; MARCUS VINICIUS DE MATOS
GOMES2
1.UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL; 2.UNIVERSIDADE NORTE DO
PARANÁ, LONDRINA, PR, BRASIL.
Introdução: Inibidores seletivos da recaptação de serotonina são a droga de escolha devido a sua
segurança e melhor tolerabilidade em relação aos antidepressivos tricíclicos. O escitalopram (ESC)
é um medicamento dessa classe e é amplamente utilizado, porém, não há relatos dos efeitos
cardiovasculares decorrentes do seu uso crônico em ratos. Objetivos: Avaliar os efeitos do
tratamento com escitalopram sobre os parâmetros basais e os componentes da análise espectral
da frequência cardíaca (VFC) e pressão sistólica (VPS). Metodologia: Foram utilizados ratos Wistar
(250-280g) tratados com veículo e escitalopram (5mg/Kg/dia), durante 21 dias, i.p. Os animais
sofreram cateterismo da artéria e veia femoral e segmentos de 5min do registro da pressão arterial
foram utilizados para a análise da VFC e VPS. Resultados: O escitalopram aumentou a pressão
arterial média (Veículo:98±2mmHg; ESC:104±2mmHg, p=0,037) e diminuiu a frequência cardíaca
(Veículo:390±3bpm; ESC:339±10bpm; p=0,005), na VFC o ESC reduziu o componente LF(nu)
(Veículo:22±2,4; ESC:14±1,6; p=0,014) e aumentou o componente HF(nu) (Veículo: 78±2,6; ESC:
85±1,6; p=0,014), logo a relação LF/HF do ESC foi reduzida (Veículo:0,32±0,05: ESC:0,18±0,025;
p=0,02).Na VPS o ESC reduziu o componente LFabs (Veículo:23,6±6,2; ESC:5,8±0,67; p=0,009).
Conclusão: O ESC interfere no sistema cardiovascular, gerando uma resposta pressora com a
presença de bradicardia, um desequilibro autonômico com predominância parassimpática e reduz
a modulação simpática vascular.
04.045 - TRATAMENTO CRÕNICO COM ESCITALOPRAM INTERFERE NA RESPOSTA
CARDIOVASCULAR CAUSADA PELO ESTRESSE DE RESTRIÇÃO AGUDO EM RATOS
DENIS CARLOS DOS SANTOS; LUIZ FERNANDO VERÍSSIMO; NATÁLIA KIMIE MATSUBARA; VIVIANE
BATISTA ESTRADA; VINICIUS LUCCA VOLPINI; GISLAINE GARCIA PELOSI; MARCUS VINICIUS DE MATOS
GOMES
UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL; .
22
Introdução: Os inibidores seletivos da recaptação de serotonina são amplamente utilizados nos
tratamentos de ansiedade e transtorno de humor; essa classe possui uma segurança cardiovascular
maior, logo, o escitalopram (ESC) é a droga de escolha em caso de pacientes com depressão e
doenças cardiovasculares, porém pouco se sabe em relação aos efeitos cardiovasculares e durante
situações aversivas, como no estresse agudo por restrição em ratos. Objetivos: Avaliar os efeitos
do tratamento com ESC sobre a função barorreflexa e as alterações cardiovasculares durante o
estresse agudo por restrição. Metodologia: Ratos Wistar (250-280g) receberam veículo e ESC
(5mg/Kg/dia), durante 21 dias, i.p, e no dia 20 sofreram cateterismo da artéria e veia femoral. O
protocolo do barorreflexo e estresse por restrição foram realizados de acordo com 1 e 2
Resultados: O ESC não alterou a resposta baroreflexa (P1, P2, Range, Ganho e BP50, p>0,05) e
reduziu a resposta pressora (Tempo: p=<0,0001, F=19,44; Tratamento: p=0,02, F=6,535;
Interação: p=0,25, F=1,143) sem alterar a resposta taquicárdica (Tempo: p=<0,0001, F=13,11;
Tratamento: p=0,99, F=<0,001; Interação: p=0,22, F=1,164) durante o estresse por restrição.
Conclusão: O escitalopram não modifica a atividade barorreflexa, porém altera a resposta
cardiovascular durante uma situação aversiva. Ref.: 1. Pelosi, G. G., et al. Autonomic
Neuroscience,131(1-2),70–6–2007; 2. Tavares, R. F. et al. Stress, 12(2),178–85–2009.
04.046 - INFLAMMATION INCREASES THE SPONTANEOUS EXCITATORY CURRENTS IN NTS
NEURONS OF RATS SUBMITTED TO SUSTAINED HYPOXIA
LUDMILA LIMA SILVEIRA; DANIELA ACCORSI-MENDONÇA; LENI GH BONAGAMBA; BENEDITO HONORIO
MACHADO
UNIVERSIDADE DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL.
Rats submitted to Sustained hypoxia (SH - 24 h) present increase of excitatory neurotransmission
in the Nucleus Tractus Solitarius (NTS) neurons, a critical synaptic station for cardiorespiratory
control. SH is also associated with inflammation in the central nervous system. Here, we evaluated
the influence of inflammation on spontaneous excitatory post-synaptic currents (sEPSCs) of NTS
neurons of rats submitted to SH. We used 4 groups of juvenile Wistar rats (100-130g): 1) normoxia
(FiO2=20.8%) treated with vehicle; 2) normoxia treated with the anti-inflammatory Minocycline
(30 mg/kg, i.p), 3) SH (FiO2=10%) treated with vehicle, 4) SH treated with Minocycline. The rats
were treated for 3 days and then exposed to SH or normoxia protocol for 24 h. Just after the
protocols, brainstem slices were obtained and whole cell patch-clamp technique was used to
record synaptic activity in the NTS. sEPSCs of NTS neurons from SH animals treated with vehicle
presented a higher frequency compared to control [5±0.7 (n=10) vs 1.5±0.3Hz (n=11)], however,
this increase was not observed in the SH group treated with Minocycline when compared to control
[2±0.5Hz (n=10) vs 1.2±0.3Hz (n=17), two-way ANOVA followed by Bonferroni’s post-test]. These
data show that inflammation triggered by sustained hypoxia contributes to the increase in
excitatory synaptic transmission in the NTS neurons of rats exposed to SH.
04.047 - EFFECTS OF LONG-TERM ELECTRICAL STIMULATION OF THE CAROTID SINUS ON
HEMODYNAMICS AND HEART RATE VARIABILITY IN RATS
PEDRO LOURENÇO KATAYAMA; JACI AIRTON CASTANIA; DANIEL P MARTINS DIAS; LUIZ EDUARDO
VIRGILIO SILVA; RUBENS FAZAN; HELIO CESAR SALGADO
FMRP/USP, RIBEIRAO PRETO, SP, BRASIL.
Electrical stimulation of the carotid sinus (ESCS) has been used for treatment of patients with
resistant hypertension. Recently, our laboratory has been conducting some studies aiming to
elucidate the mechanisms underlying this approach. The aim of the present study was to
investigate the effects of 3 days of ESCS on hemodynamics and heart rate variability (HRV) in
conscious rats. Wistar rats were instrumented with a bipolar electrode, attached to an
implantable electrical stimulator, around the left carotid sinus and a catheter into the femoral
artery. On the next day, the pulsatile arterial pressure (PAP) was recorded during 60 min. Next,
the electrical stimulator was activated and a protocol for intermittent ESCS (30Hz; 1ms; 3V; 20s
ON; 20s OFF) was carried out during 3 days (stimulators remained activated). PAP and electrical
stimulation signals were recorded every day during 15 min. Finally, after deactivation of the
electrical stimulator the PAP was recorded during 60 min. Results showed that ESCS was efficient
to reduce mean arterial pressure in comparison to baseline values (81 vs 106 mmHg) only during
the first day. ESCS caused no changes in heart rate. HRV analysis showed that, as compared to
baseline, after 3 days of ESCS there was an increase in parasympathetic modulation, expressed by
a rise in the HF power (81 vs 72 nu), combined with a decrease in sympathetic modulation
expressed by the reduction in LF power (19 vs 27 nu) and LF/HF (0.3 vs 0.5).
04.048 - ANALYSIS OF HEART RATE VARIABILITY BY MULTISCALE ENTROPY IN SPONTANEOUSLY
HYPERTENSIVE RATS
LUIZ EDUARDO VIRGILIO SILVA; CARLOS ALBERTO AGUIAR SILVA; HELIO CESAR SALGADO; RUBENS
FAZAN
FMRP/UNIVERSIDADE DE SÃO PAULO, RIBEIRAO PRETO, SP, BRASIL.
Most approaches commonly used to access heart rate variability (HRV) are based on linear models,
such as spectral analysis. Recently, much attention has been given to nonlinear methods as they
are able to quantify additional important properties of HRV dynamics. Multiscale entropy (MSE)
can quantify the predictability of time series over several time scales, which is demonstrated to
be related to neuro-humoral regulation of cardiac activity. We applied MSE (scales 1-20) on
spontaneously hypertensive rats (SHR) in order to identify how hypertension affect predictability
of HR over several scales (complexity). ECG were continuously recorded during 90 minutes in SHR
(N=7) and normotensive Wistar-Kyoto rats (WKY, N=6) previously (5 days) instrumented with
probes that allowed wireless ECG recordings. The interval between consecutive R waves (RR) were
identified and the time series of RR were extracted and processed (artifact and missed detections
excluded) prior to analysis. Entropy values of SHR are significantly lower compared to WKY for
scales four onwards. Total entropy is also significantly lower in SHR (11.6 ± 0.9 vs 16.7 ± 1.2,
p=0.008). MSE curve in SHR showed a rapid decrease reaching a plateau after scale 3. In WKY the
decrease of MSE was smaller than in SHR and it fully recovers towards the higher scales. Results
suggest that long-term mechanisms are the most affected by hypertension, likely due to increased
sympathetic activity and humoral effects.
04.049 - EXERCÍCIO FÍSICO MODULA A ATIVIDADE BARORREFLEXA APÓS ESTRESSE DE RESTRIÇÃO
AGUDO
VINICIUS LUCCA VOLPINI1; GISLAINE GARCIA PELOSI1; MARCUS VINICIUS DE MATOS GOMES2; LUIZ
FERNANDO VERÍSSIMO1; NATÁLIA KIMIE MATSUBARA1; DENIS CARLOS DOS SANTOS1; CARLOS
HENRIQUE PESSOA1; VIVIANE BATISTA ESTRADA1
1.UNIVERSIDADE ESTADUAL DE LONDRINA (UEL), LONDRINA, PR, BRASIL; 2.UNIVERSIDADE NORTE
DO PARANÁ (UNOPAR), LONDRINA, PR, BRASIL.
INTRODUÇÃO: O estresse tem sido descrito como um fator de risco para doenças cardiovasculares,
intensificando respostas simpáticas, contribuindo para o aumento da contratilidade e frequência
cardíaca. O exercício físico modifica a resposta cardiovascular de ratos, gerando uma bradicardia
de repouso e atenuação da atividade simpática. OBJETIVO: Nosso objetivo foi investigar a resposta
barorreflexa em ratos treinados após o estresse agudo de restrição. MATERIAS/MÉTODOS: Ratos
machos foram treinados no protocolo de natação de 4 semanas (1 hora/dia). Foi realizado o
cateterismo de artéria e veia femorais. No dia seguinte, foi avaliada a resposta barorreflexa,
através da infusão i.v. de drogas vasoativas (fenilefrina e nitroprussiato) antes (situação basal) e
após um estresse de restrição (imediatamente e 30 minutos após). RESULTADOS: Os parâmetros
da sigmóide gerada pelo barorreflexo mostraram-se alterados entre os grupos. O grupo treinado
(TE) mostrou um menor ganho que o grupo sedentário (SE) na situação basal (SE: g=-1,8 ±0,2; n=9;
TE: g=-1,5±0,1; n=7; t= 3,07; p=0,01). Trinta minutos após o estresse o grupo TE obteve o P1
menor que o SE (SE: P1=108±10 mmHg; n=8; TE: P1=72±10 mmHg; n=6; t= 2,54; p=0,02), e o P2
maior (SE: P2=-174±12 mmHg; n=8; TE: P2=-107±20 mmHg; n=6; t= 3,03; p=0,01), e um range
menor (SE: R=281±9; n=8; TE: R=178±24; n=6; t=4,46; p=0,01). CONCLUSÃO: Logo, os resultados
sugerem que o grupo TE obteve uma melhor adaptação do barorreflexo à situação de estresse do
que o grupo SE.
04.050 - PHYSICAL PERFORMANCE IS ASSOCIATED WITH BLOOD PRESSURE AND HEART RATE
VARIABILITY IN RATS
WESLLEY MOURA SANTOS1; SAMUEL PENNA WANNER1; MILENE RODRIGUES MALHEIROS2; CÂNDIDO
CELSO COIMBRA1; NILO RESENDE VIANA LIMA1; WASHINGTON PIRES1
1.UFMG, BELO HORIZONTE, MG, BRASIL; 2.USP, SÃO PAULO, SP, BRASIL.
The present study investigated whether running performance in both temperate and warm
environments is associated with blood pressure and heart rate variability parameters. Male Wistar
rats had a catheter implanted into their aorta to record pulsatile arterial pressure. After
recovering from this surgery, the rats were subjected to incremental-speed exercises (starting at
10 m/min, with increments of 1 m/min every 2 min) performed until volitional fatigue under
temperate (25°C) and warm (35°C) conditions. Time- and frequency-domain analyses were
evaluated during the initial stages of the incremental exercises. The spectral power components
for very low- (VLF, 0.02 - 0.20 Hz), low- (LF, 0.20 - 0.75 Hz), and high-frequency (HF, 0.75 - 3 Hz)
bands were evaluated. The maximal speed attained by the rats during the incremental exercise
was lower at 35°C than at 25°C (22 ± 2 m/min vs 29 ± 3 m/min, p<0.05). In both ambient, exercise
performance was negatively correlated with the variance of the systolic blood pressure (r = -0.89
and -0.82 for temperate and warm, respectively; p<0.05) and with the VLF blood pressure
oscillations (r = -0.83 and -0.87 for temperate and warm, respectively; p<0.05). Finally, running
performance was associated with LF/HF ratio of the heart rate variability, an index of cardiac
sympathetic activity. In conclusion, physical performance in rats subjected to incremental
exercises was associated with the changes in their blood pressure and heart rate variability.
04.051 - EVIDENCES THAT RESPIRATORY NETWORK MODULATES THE SYMPATHETIC ACTIVITY IN
RATS SUBMITTED TO SINO-AORTIC DENERVATION
MATEUS RAMOS AMORIM; GEORGE MPR SOUZA; DAVI JA MORAES; BENEDITO HONORIO MACHADO
Several days after sino-aortic denervation (SAD) rats present normal levels of sympathetic activity
and mean arterial pressure (MAP). However, the mechanisms involved in the modulation of the
sympathetic activity after SAD remain unclear. We hypothesized that the respiratory network may
contribute to the modulation of the pre-sympathetic neurons preventing increases in MAP in SAD
rats. To elucidate this mechanism we recorded the activities of phrenic and thoracic sympathetic
(tSNA) nerves in male juvenile Wistar rats in the working heart-brainstem preparation, at high or
low respiratory drive induced by hypercapnia or hypocapnia, respectively. The duration of
inspiration significantly increased in SAD rats (1,46 ± 0,05 vs 1,14 ± 0,07 s), and there was no
change in the baseline tSNA in all phases of respiration [SAD (n=23) and Sham (n=28)]. Hypercapnia
increases tSNA in all phases of respiration in Sham rats (n=13), but in SAD rats (n=14) a significant
increase in tSNA was observed only during inspiration (43 ± 3% vs 55 ± 3%). Hypocapnia induced
sympatho-inhibition in SAD (n=8) and in Sham rats (n=10). The data show that SAD rats present
changes in the neural respiratory network, which may contribute to the modulation of the
sympathetic activity in the absence of arterial baroreceptors.
04.052 - EFFECTS OF EXOGENOUS L-ARGININE ON FLOW MEDIATED DILATION AFTER EXPOSURE
TO HIGH LEVELS OF RETROGRADE SHEAR RATE
GABRIEL MATHEUS DA SILVA BATISTA; DANIEL GALINIS VIEIRA LIMA; HELENA NALY MIGUENS ROCHA;
VINICIUS PACHECO GARCIA; GUSTAVO MATARUNA DA SILVA; JOÃO DARIO MARTINS MATTOS;
MONIQUE OPUSZCKA CAMPOS; IGOR ALEXANDRE FERNANDES; ANTONIO CLAUDIO LUCAS DA
NÓBREGA; NATÁLIA GALITO ROCHA
UNIVERSIDADE FEDERAL FLUMINENSE, NITERÓI, RJ, BRASIL.
Increased retrograde shear rate (RSR) has been related to impairment in flow-mediated dilation
(FMD), which might be associated to a decrease in nitric oxide (NO) bioavailability. Thus, we
sought to examine the effects of exogenous L-arginine infusion, a NO precursor, on brachial artery
reactivity after exposure to increased RSR. Eleven healthy men (32±7yr) underwent to two
different conditions in which a 30-min intravenous infusion of either saline (SAL) or L-arginine
(ARG) was conducted. An occlusion cuff was inflated (220 mmHg) on the fist of their dominant
arm to induce an increment of RSR during the last 20 minutes of each infusion. Vascular reactivity
was determined by brachial artery FMD at baseline and immediately after inflating the occlusion
cuff. RSR increased as a consequence of the cuff occlusion during both SAL and ARG infusions
(means±SE; baseline: 9.7±3.2 vs. SAL: 16±4.5; baseline: 10.5±3.8 vs. ARG: 32.2±7.5; P<0.05). After
deflating the occlusion cuff, brachial artery diameter was reduced in the condition that SAL was
infused (baseline: 0, 39±0.01 vs. SAL: 0,38±0,01; P≤0.01), while ARG infusion avoided this
reduction (SAL: 0.38±0.01 vs. ARG: 0.39±0.01; P=0.02 vs. saline). FMD was not different before
and after cuff occlusion in both SAL and ARG conditions (P>0.05). In conclusion, ARG infusion
prevented the increased RSR-induced reduction on arterial diameter, but it did not affect vascular
reactivity.
04.053 - ISCHEMIC PRECONDITIONING IMPROVES CARDIAC AUTONOMIC MODULATION IN
HEALTHY SUBJECTS: A RANDOMIZED AND PLACEBO/NOCEBO CONTROLLED STUDY
TIAGO OBEID-FREITAS1; JEANN LUCCAS DE CASTRO SABINO-CARVALHO1; MARCELLE DE PAULA
RIBEIRO1; THIAGO RIBEIRO LOPES2; THIAGO HENRIQUE NUNES FERREIRA1; JOSE ERNESTO SUCCI1;
ANTONIO CARLOS SILVA1; BRUNO MOREIRA SILVA1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO PAULO, SP, BRASIL; 2.CENTRO OLÍMPICO DE
TREINAMENTO E PESQUISA, SÃO PAULO, SP, BRASIL.
Ischemic preconditioning (IPC; brief ischemia followed by reperfusion) protects against ischemiareperfusion injury, which is mediated in part by the autonomic nervous system. However, it is
unknown whether IPC can also improve the autonomic modulation in a healthy state. Thus, we
submitted six healthy high-level runners (i.e. presumably pursue an optimal autonomic
modulation) to three interventions, in random order (IPC, cuffs positioned on the thighs; SHAM,
fake ultrasound session; CT, resting control). Subjects were told that both IPC and SHAM would
improve autonomic modulation compared to CT (i.e., we sought to induce a similar placebo, while
avoiding a nocebo effect). Volunteers rested supine for 5 min and then cardiac autonomic
modulation was assessed by respiratory sinus arrhythmia (RSA, six respiratory cycles) and heart
rate variability (HRV, 5-min recording) at pre and post-interventions. At post-intervention, RSA
did not change (P > 0.50 vs. pre). But, Low Frequency from HRV increased in IPC (124% vs. pre, P
= 0.01) and SHAM (162% vs. pre, P = 0.04). RMSSD and High Frequency from HRV tended to increase
at post intervention in IPC (43% vs. pre, P = 0.07 and 77% vs. pre, P = 0.06, respectively). In
summary, these preliminary results indicate the IPC acutely improved cardiac autonomic
modulation in healthy subjects, but part of this improvement was mediated by a placebo effect.
04.054 - SYMPATHETIC OVERACTIVITY AND HYPERTENSION IN RATS SUBMITTED TO CHRONIC
INTERMITTENT HYPOXIA ARE NOT DEPENDENT OF MEDULLARY C1 NEURONS
DAVI JA MORAES1; LENI GOMES HECK BONAGAMBA1; JULIAN F R PATON2; BENEDITO HONORIO
MACHADO1
1.USP, RIBEIRAO PRETO, SP, BRASIL; 2.UNIVERSITY OF BRISTOL, BRISTOL, REINO UNIDO.
Chronic intermittent hypoxia (CIH) produces respiratory-related sympatho-excitation in rats,
which might be an important contributor to the development of hypertension. We evaluated the
role of sympatho-excitatory cathecolaminergic medullary C1 neurons in the CIH-induced
respiratory-related sympatho-excitation and hypertension in rats. In awake rats in vivo and in in
situ perfused preparations of rat, C1 neurons were acutely silenced by application of the insect
peptide allatostatin following cell-specific targeting with a lentiviral vector to express the
inhibitory Drosophila allatostatin receptor in control and CIH rats (10 days). In awake rats,
inhibition of ~ 82% of the C1 neurons resulted in a profound and similar fall in arterial pressure in
control (n=12) and CIH rats (n=12). However, CIH rats still presented elevated arterial pressure
after C1 inhibition (p<0.05). In in situ, C1 inhibition resulted in reversible reductions of perfusion
pressure, amplitude of respiratory-related bursts of sympathetic nerve and C1 pre-sympathetic
neurons activities in control (n=7) and CIH (n=9) rats. However, CIH-, acute hypoxia- or CO2induced respiratory-related sympatho-excitation were not affected by C1 inhibition. These data
confirm a physiological role of C1 neurons in regulating sympathetic activity and arterial pressure.
However, C1 neurons apparently are not involved in the respiratory-related sympatho-excitation
and hypertension evoked by CIH.
23
04.055 - PAPEL DO RECEPTOR COLINÉRGICO NICOTÍNICO ALFA-7 NO REMODELAMENTO
CARDÍACO INDUZIDO POR CONSTRIÇÃO DA AORTA TRANSVERSA
THAIS CRISTINA ALCÂNTARA; CIBELE ROCHA RESENDE; ANA CAROLINA FERREIRA FARIA; ISABELA
CRISTINA MAGALHÃES; SÉRGIO RICARDO ALUOTTO SCALZO JÚNIOR; JULLIANE VASCONCELOS
JOVIANO DOS SANTOS; MAÍSA MOTA ANTUNES; MARCOS BARROUIN MELO; GUSTAVO BATISTA
MENEZES; SILVIA CAROLINA GUATIMOSIM FONSECA
A inflamação participa na patogênese das doenças cardíacas. Um importante mecanismo de
controle da inflamação é a via anti-inflamatória colinérgica, na qual a acetilcolina inibe a
expressão de citocinas e quimiocinas pró-inflamatórias ao se ligar a receptores colinérgicos
nicotínicos alfa-7 (α7nAChR). O objetivo desse trabalho foi avaliar o impacto da ausência do
α7nAChR nas alterações cardíacas desenvolvidas após cirurgia de constrição da aorta transversa
(TAC). Para isso, foram utilizados camundongos selvagens (WT) e nocautes para o α7nAChR (Alfa7)
divididos em WT SHAM, WT TAC, Alfa7 SHAM e Alfa7 TAC. Ecocardiografia mostrou disfunção
contrátil e dilatação do ventrículo esquerdo no grupo Alfa7 TAC com 6 semanas após a TAC. Além
disso, apenas camundongos Alfa7 TAC apresentaram hipertrofia do ventrículo esquerdo 1 semana
após a TAC, sendo também observado aumento do diâmetro dos cardiomiócitos. Nesse ponto,
coração de camundongos Alfa7 TAC apresentou redução na expressão da SERCA e na fosforilação
da fosfolambam por meio da técnica de western blot; enquanto que um aumento na expressão da
SERCA foi observado no grupo WT TAC. Além disso, foi detectado aumento de expressão da
subunidade p65 de NF-κB e aumento no peso do baço apenas no grupo de camundongos Alfa7 TAC.
Esses resultados mostram que o α7nAChR participa na modulação do remodelamento cardíaco
induzido por sobrecarga pressórica, sendo essencial para a manutenção da função cardíaca após
a TAC.
04.056 - EXERCISE AEROBIC ASSOCIATED TO TREATMENT WITH DIMINAZENE ACETURATE
REDUCES INTRINSIC HEART RATE IN SPONTANEOUSLY HYPERTENSIVE RATS
PAULO RICARDO LOPES1; MARINA CONCEIÇÃO DOS SANTOS MOREIRA1; LARISSA MATUDA MACEDO1;
MARCOS FERREIRA NETO2; CARLOS HENRIQUE DE CASTRO1; GUSTAVO RODRIGUES PEDRINO1
1.UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL; 2.UNIVERSIDADE FEDERAL DE
UERLÂNDIA, UBERLÂNDIA, MG, BRASIL.
Physical training has been pointed out as an efficient non-pharmacological treatment to
hypertension. The present study sought to determine the effects of the aerobic training on a
treadmill associated with administration of diminazene (DIZE) on intrinsic heart rate in
spontaneously hypertensive rats (SHR). Male SHR with 12 weeks of age were used. One group was
undergone to eight weeks of training on a treadmill (TR). Another group remained without physical
activity (SD). In the last 15 days of training, the animals were redistributed in four groups, where
SD and TR received DIZE (1 mg/kg; ig) or distilled water (vehicle) by gavage. It was performed tail
plethysmography before and during the treatment. After the treatment, the animals were
anesthetized and submitted to artery and femoral vein cannulation surgery. After 24 hours, the
animals were submitted to HR (heart rate) record and double autonomic blockade (DBA). The HR
values of the TR rats were lower when compared to SD rats (357.7 ± 2.5; n=10 vs. 385.7 ± 3.6; n=8
bpm) before the beginning of the treatment with DIZE. At the end of the treatment, the IHR of
the TR rats receiving DIZE was lower when compared with the IHR of the SD rats that also received
DIZE (n=4, 363.9 ± 3.7bpm vs. n=5, 336.5 ± 22.7bpm; p<0.05) after the DBA. We conclude that the
physical training in treadmill has been able of promoted an intrinsic bradycardia in TR SHR, when
treated with DIZE at a dose of 1 mg/kg.
04.057 - ADRENERGIC BLOCKADE WITH BUCINDOLOL DECREASED RIGHT VENTRICLE
DYSFUNCTION AND PULMONARY EDEMA IN PULMONARY HYPERTENSIVE RATS
BRUNA GAZZI DE LIMA SEOLIN; RAFAEL COLOMBO; RAYANE BRINCK TEIXEIRA; JÉSSICA H.POLETTO
BONETTO; LUIZA MEZZOMO DONATTI; ADRIANE BELLÓ-KLEIN
UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL (UFRGS), PORTO ALEGRE, RS, BRASIL.
INTRODUCTION: Pulmonary hypertension (PH) is associated to right ventricle (RV) hypertrophy.
The blockade of adrenergic receptors with bucindolol (BCD) decreases right ventricular
hypertrophy. AIM: To evaluate the effect of BCD on hemodynamics and RV structure in the
monocrotaline (MCT) model of PH. METHODS: Male Wistar rats were divided into four groups
(n=10/group): control (CTR), MCT, BCD (0.9% NaCl i.p.) and MCT+BCD (60mg/kg i.p.). After 2
weeks of injection, they were treated with BCD (2mg/kg/day i.p.) or vehicle for 7 days. RESULTS:
MCT+BCD decreased pulmonary congestion (22%) compared to MCT. MCT groups showed increase
in RV/body weight and RV/tibia lenght ratios as compared to CTR. It was observed a reduction in
RV systolic pressure (36%), in RV end diastolic pressure (59%), in maximum derivative (20%) and in
minimum derivative (26%) in MCT+BCD as compared to MCT. In both groups treated with BCD RV
systolic diameter decreased as compared to MCT (28%) and CTR (21%) groups. RV systolic area and
mean pulmonary artery pressure decreased in MCT+BCD (40%) compared to MCT (32%). It was
noted an increase (46%) in the RV fractional area change, in tricuspid annular plane systolic
excursion (28%), in RV fractional shortening (61%), in systolic volume (28%) and in cardiac output
(20%) in MCT+BCD compared to MCT. CONCLUSION: BCD significantly improved the right ventricle
function and decreased pulmonary congestion in rats with HP.
04.058 - CONTROL OF SYMPATHETIC ACTIVITY BY A5 NORADRENERGIC NEURONS IN THE IN SITU
RAT PREPARATIONS
CAMILA LINHARES TAXINI; LUCIANE HELENA GARGAGLIONI BATALHAO; DANIEL BRESEGHELLO
ZOCCAL
SÃO PAULO STATE UNIVERSITY (UNESP), JABOTICABAL, SP, BRASIL.
The A5 area contains noradrenergic neurons that establish connections with brainstem areas
involved in the cardiorespiratory control. Herein, we evaluated the involvement of A5
noradrenergic neurons in the processing of central and peripheral chemoreflex sympathetic
responses using the in situ working heart-brainstem rat preparation. Juvenile male Holztman rats
received bilateral microinjections of either IgG-SAP (50nl, n=7) or anti-dopamine betahydroxylase-saporin (anti-DβH-SAP, 4.2 ng/50 nl, n=6) in the A5. One week later, in situ
preparations were obtained to record the thoracic sympathetic (tSN) and phrenic nerve (PN)
activities. Baseline tSN activity (12±2 vs 13±2 μV), PN burst amplitude (41±10 vs 45 ±20 μV) and
frequency (13±1 vs 15±2 bpm) and the respiratory-sympathetic coupling pattern were similar
between groups. The sympathetic (∆tSN: 110±12 vs 58±8 %, P<0.05), but not the phrenic response
to peripheral chemoreflex stimulation (KCN, 0.05%, 50nL) was marked attenuated in animals with
lesion of A5 noradrenergic neurons. As to the central chemoreflex, the tSN response to 7% CO2
(∆tSN: 9.5±1.4 vs 3.9±1.7%, P<0.05), but not to 10% CO2 (16.4±2.9 vs 10.9±1.6%) was attenuated
in A5-lesioned rats in comparison to controls. The PN response to 7 and 10% CO2 were similar
between groups. Our data show that the A5 noradrenergic neurons are critical for the full
expression of the sympathetic chemoreflex responses.
04.059 - EVALUATION OF THE EFFECTS OF MAGNETIC NANOPARTICLES COATED WITH ALBUMIN
IN CARDIOVASCULAR FUNCTION IN RATS
LORRANY APARECIDA GOMES SILVA; ALLANCER DIVINO CARVALHO NUNES; NICHOLAS ZUFELATO;
ELIZABETH PEREIRA MENDES; DIEGO BASILE COLUGNATI; ANDRIS FIGUEIROA BAKUZIS; CARLOS
HENRIQUE DE CASTRO
Magnetic Nanoparticles (MNPs) have been used for various biomedical applications. Previous
studies have shown that the some effects of MNPs are related to the coating surface. The aim of
this study was to evaluate the cardiovascular effects of different types of manganese ferritebased magnetic nanoparticles: citrate-coated (CiMNPs); bare magnetic nanoparticles (BaMNPs);
albumin-coated (AlbMNPs); and citrate albumin-coated (CiAlbMNPs). Hearts were perfused
according Langendorff method. After a basal period (30 min), hearts were perfused with MNPs for
10 minutes following 20 min of washout. Femoral vein and artery were cannulated to infuse MNPs
and record arterial blood pressure in conscious rats. Statistical differences were considered
significant if p<0.05. The CiMNPs induced a slight decrease in the contractility, which was reversed
in the washout period. This effect was not different in CiAlbMNPs perfused hearts. BaMNPs
promoted an increase in the left ventricular end-diastolic pressure and coronary perfusion
pressure and decreased left ventricular developed pressure, dP/dt max and dP/dt min.
Interestingly, these effects were not observed in the AlbMNPs perfused hearts. Any MNPs were
able to change heart rate or arterial pressure in conscious rats. In summary, our data suggest that
the coating MNPs with albumin inhibit MNPs-induced cardiac effects and might be an important
strategy to improve the use of the MNPs in biomedical applications.
04.060 - MEDULLARY RESPIRATORY NEURONS ACTIVITY IN JUVENILE FEMALE RATS EXPOSED TO
CHRONIC INTERMITTENT HYPOXIA
GEORGE MIGUEL PERBONE ROBUSTE SOUZA; MATEUS RAMOS AMORIM; DAVI JA MORAES; BENEDITO
HONORIO MACHADO
Resumo:
Juvenile female rats submitted to chronic intermittent hypoxia (CIH) present sympathetic
overactivity during the inspiratory phase of the respiratory cycle. Considering that the increase in
sympathetic activity in CIH-female rats is observed during the inspiratory phase and also that the
pre-sympathetic neurons are modulated by the respiratory network, we aimed to explore the
medullary respiratory neurons activity in juvenile female rats submitted to CIH. To reach this
goal, juvenile female rats were exposed to 10 days of CIH or normoxia. After this period,
extracellular single unit recordings of medullary respiratory neurons were performed using the in
situ working heart brainstem preparation. Among the respiratory neuron types evaluated, we
recorded the activity of 2 types of expiratory and 1 type of inspiratory neurons. During expiration,
the firing frequency of Post-I neurons was not different between CIH (n=3) and control (n=3) group
(46,4 ±13,6 vs 24,2 ±8,1 Hz) and the firing frequency of Aug-E neurons was not different between
CIH (n=3) and control (n=4) group (60,1 ±24,6 vs 62,2 ±15,66 Hz). During inspiration, the firing
frequency of Ramp-I neurons was not different between CIH (n=6) and control (n=3) group (125,1
±27,6 vs 88,6 ±25,6 Hz). These results show that sympathetic overactivity observed during the
inspiratory phase of the respiratory cycle in CIH-female rats is not associated with changes in PostI, Aug-E or Ramp-I neurons activity. Further experiments are required for evaluation of other
subtypes of respiratory neurons in CIH-female rats.
24
arterial infusions of HS (0.175; 0.3; 0.7; 1.5 e 2 M NaCl; 200 µL each 20 seconds) were done in
ascending order. In sham rats, only intra-arterial infusion of 2 M NaCl increased tSNA (69.8±17.6%,
vs. Ringer: -3.1±3.4%, p<0.05) and PNA (6.1±1.5 bpm, vs. Ringer: -0.1±0.5 bpm, p<0.05). Carotid
body denervation impaired the increase in the tSNA (22.47±4.6%, p<0.05) induced by 2 M NaCl,
without significantly changing the tachypnea (2.7±0.3 bpm, p<0.05). The removal of the forebrain
(decerebrated rats) abolished the changes in the tSNA (8.4±3.7%) or PNA (1.2±0.4 bpm). The
increase in the perfusate osmolality was similar in all groups at the end of the tests (11
mosmol.kg.H2O-1). The results suggest the involvement of the forebrain and carotid body in the
sympatho-excitatory response, while the tachypnea appears to depend only on forebrain areas.
04.065 - BOMBESIN RECEPTOR INHIBITION INTO ROSTRAL VENTROLATERAL MEDULLA
PROMOTES HYPOTENSION AND RENAL SYMPATHOINHIBITION IN HYPERTENSIVE RATS
IZABELLA SILVA DE JESUS PINTO; ALINE ANDRADE MOURAO; DIEGO BASILE COLUGNATI; GUSTAVO
RODRIGUES PEDRINO
UFG, GOIANIA, GO, BRASIL.
Recently our laboratory demonstrated that microinjection of bombesin in rostral ventrolateral
medulla (RVLM) induced hypertension and sympathetic hyperactivity. However, bombesin
receptor inhibition did not cause changes in the autonomic and cardiovascular parameters of
normotensive rats. The present study sought to investigate the autonomic and cardiovascular
effects induced by bombesin receptor antagonist into RVLM of spontaneously hypertensive rats
(SHR). Male SHR rats (250-350g) were instrumented for mean arterial pressure (MAP), heart rate
(HR) and renal sympathetic nerve activity (RSNA) records and for microinjections of saline (NaCl;
150 mM) and bombesin receptor antagonist (RC-3095; 3.10-6 M) into RVLM. In anesthetized rats
(n=4), bombesin receptor antagonist bilateral microinjections produced hypotension (-14.02 ± 2.4
mmHg; -16.80 ± 2.4 mmHg) and decrease of RSNA (-18.80 ± 4.7%; -14.59 ±4.2 %) when compared
to the saline microinjection, MAP (-0.27 ± 1.8 mmHg), HR (0.25 ± 1.6 bpm) and RSNA (2.15 ± 1.1
%). The results demonstrated that antagonist RC-3095 microinjections promoted autonomic and
cardiovascular responses in hypertensive rats. These findings suggest that the RVLM contains
specific neuropeptides and these could be further active in SHR.
04.061 - HIGH SALT DIET FOR 12 WEEKS AFTER WEANING CHANGES THE CONTROL OF BLOOD
PRESSURE IN RATS DEPENDENT ON CENTRAL PATHWAYS ACTIVATED BY ENDOGENOUS
ANGIOTENSIN II
PAULA MAGALHÃES GOMES; LUCIANA NEVES FARIA; RENATO WILLIAN MARTINS SÁ; ERIC MORAIS DA
COSTA BRAGA; GIOVANA LOPES AGUIAR; ANDREIA CARVALHO ALZAMORA; LISANDRA BRANDINO DE
OLIVEIRA; LEONARDO MÁXIMO CARDOSO
High salt diet is classically involved with the development of hypertension in experimental models.
However, very few studies addressed the question whether high salt diet from weaning to adult
life in fact lead to hypertension. Therefore, we sought to investigate if high salt diet from weaning
to adult life increase blood pressure. Male Wistar rats were fed either high (2%, HS), n = 10, or
regular (0.4%, Cont), n = 13, salt diets for 12 weeks after weaning. Stainless steel cannula were
implanted directed to the lateral ventricle and after five days, femoral vein and artery were
cannulated. Vehicle or losartan (30µg/kg) were injected in 1μL, separated each other by 1 hour.
Hexamethonium (3mg/kg and 30mg/kg) was given at the end of the experiment. Mean (MAP),
systolic (SBP) and diastolic blood pressure (DBP) levels were higher in high salt consuming rats
compared to control (131.5±2.9 mmHg vs. 117.6±2.5 mmHg, MAP; 151.7±4.4 mmHg vs. 135.1±3.5
mmHg, SBP; 117.6±4.5 mmHg vs. 101.2±2.8 mmHg, DBP). Central losartan reduced blood pressure
of HS rats. Hexametonium (3mg/kg) produced a fall 185.32% higher in HS rats (-12.83±1.2 mmHg)
compared to Cont rats (-6.92±1.3 mmHg). Current data suggests that high salt diet after weaning
may affect blood pressure control leading to mild hypertension in a way that may depends on
central pathways activated by endogenous angiotensin II and sympathetic drive to the
cardiovascular system.
04.066 - INTRACEREBROVENTRICULAR MICROINJECTION OF BLOCKER AND OPENER OF KATP
CHANNELS COULD NOT REACH ANY ALTERATION ON AUTONOMIC CARDIOVASCULAR OUTFLOW
BRUNO PAES LEME FERREIRA; RAONI DA CONCEIÇÃO DOS SANTOS; LÍVIA DA ROCHA NATALINO
MONTEIRO; RAQUEL DO NASCIMENTO DE SOUZA; ANDRÉ DE SOUZA MECAWI; LUIS CARLOS REIS;
LUCIANO GONÇALVES FERNANDES
UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, SEROPEDICA, RJ, BRASIL.
ATP sensitive potassium channels (KATP) in glucosesitive neurons of hypothalamus play a key role
in organic functions such as control of food intake. However, the involvement of such channels in
the autonomic outflow to the cardiovascular system is poorly understood. Material and Methods:
Male Wistar rats (250-300g) were submitted to stereotaxic surgery for guide cannula placement
into the third ventricle (AP=1,8mm, DV=8,2mm). Seven days after, animals underwent to a
electrocardiographic (ECG) record, before and after drug microinjections of vehicle (Molecusol
5%, n=6), glibenclamide (5mM, a KATP channel blocker, n=10) or diazoxide (25nM, a KATP channel
opener, n=10). Heart rate variability of the ECG was analyzed by Fast Fourier Transform and
statistical analysis was performed by One-Way ANOVA test. Data shown as mean ± SEM. Ethical
Committee in research protocol: UFRRJ006247/2012. Results: There was no statistical difference
between the basal and experimental periods regarding low frequency in vehicle (65.17±5.369n.u.
vs 62.17±5.167n.u.), glibenclamide (53.50±5.350n.u. vs 60.40±4.566n.u.) or diazoxide
(51.80±3.571n.u. vs 56.10± 5.067n.u.) groups. Also, no difference was found regarding high
frequency HRV component (vehicle 34.83±5.369n.u. vs 37.83±5.167n.u.; glibenclamide
46.50±5.350n.u. vs 39,60±4.566n.u. and diazoxide 48.20±3.571n.u. vs 43.90± 5.067n.u.).
Conclusion: Activation or blockade of the KATP channels by the microinjection of its agonists or
antagonists in the third ventricle failed to promote any change in heart rate variability.
04.062 - THE IMPACT OF GENDER AND AEROBIC EXERCISE TRAINING ON REDOX STATE AND
AUTONOMIC CARDIOVASCULAR CONTROL IN HEALTHY MIDDLE-AGED ADULTS
MAYCON JUNIOR FERREIRA1; CARLOS HENRIQUE GROSSI SPONTON2; RODRIGO DEGLI ESPOSTI1;
ALINE PINCERATO JARRETE1; ANGELINA ZANESCO1
1.UNESP, RIO CLARO, SP, BRASIL; 2.UNICAMP, CAMPINAS, SP, BRASIL.
The aim of this study was to evaluate the effects of gender and aerobic exercise training (AET) on
autonomic function parameters as well as redox state markers in perimenopausal women (PW)
and men (M) counterparts. Fifteen PW (47.8 ± 0.9 yrs) and sixteen M (50.7 ± 1.2 yrs) underwent
24 sessions of AET at intensity of maximal lactate steady state (3 days/wk, 30 – 40 min). Resting
heart rate (HR) and heart rate variability were measured using a beat-to-beat HR monitor. Blood
pressure (BP) was measured by auscultatory and ambulatory methods. BP variability was
calculated by average real variability index. Biochemical (NOx- and malondialdehyde - MDA levels)
and antioxidant enzyme activities (superoxide dismutase - SOD and catalase) were analyzed using
commercial kits. Statistical analysis were Anova two-way followed by Bonferroni post hoc. The
PW group showed lower iRR mean, RMSSD, LF and TP values compared to the M group. PW showed
lower systolic and diastolic ambulatory BP and lower nighttime diastolic BP variability. In addition,
the PW group had higher resting HR than M group. On the other hand, PW group showed lower
catalase activity. No differences were found for NOx- and MDA levels even as SOD activities
between groups. Unexpectedly, AET did not change any of the parameters in both groups. In
summary, the gender effects were more pronounced than AET on autonomic cardiovascular
control and redox state, however were not associated with the BP.
04.067 - A RISE IN PERIPHERAL RESISTANCE ACCOUNTS FOR MORTALITY IN ENDOTOXIC SHOCK
CAMILA F BRITO; ELIZABETH A FLATOW; ALEXANDRE A STEINER
INSTITUTO DE CIÊNCIAS BIOMÉDICAS, SAO PAULO, SP, BRASIL.
Whereas severe endotoxemia causes a progressive fall in the arterial pressure of anesthetized
rats, it rarely does so in conscious rats. The hypotensive response of conscious rats to endotoxemia
is usually transient, and mortality in such rats often occurs when they are normotensive. To
investigate this issue, we performed a detailed hemodynamic assessment of conscious rats
challenged with endotoxin (lipopolysaccharide, LPS) at a high dose (500 g/kg iv). LPS induced
two transient falls in arterial pressure, with nadirs at 10 and 80 min. Both phases of hypotension
were brought about by reductions in stroke volume and systemic peripheral resistance, the only
difference being that heart rate was elevated during the first phase but not during the second
phase. Notably, during the recovery from the second phase, peripheral resistance became
elevated as stroke volume continued to fall. The elevation in peripheral resistance and the fall in
stroke volume were progressively amplified as arterial pressure was maintained during the posthypotension period. These progressive changes culminated in the death of the animals at 150-180
min. Therefore, we propose that a baroreceptor-driven rise in peripheral resistance is maladaptive
in endotoxic shock, similarly to what happens in the pathophysiology of heart failure.
04.063 - PAROXETINE INTRACEREBROVENTRICULAR AND CARDIAC PARAMETERS IN AORTIC
REGURGITATION
PALOMA GRAZIELE BITTENCOURT DA SILVA1; SILMÉIA GARCIA ZANATI 2; JULIANA IRANI FRATUCCI DE
GOBBI1
1.DEPT. OF PHYSIOLOGY, INSTITUTE OF BIOSCIENCE - UNESP BOTUCATU, BOTUCATU, SP, BRASIL;
2.DEPT. INERNAL MEDICINE, BOTUCATU MEDICAL SCHOOL, UNESP, BOTUCATU, SP, BRASIL.
Aortic regurgitation (AR) leads to eccentric hypertrophy and eventually left ventricular
dysfunction. Rheumatic heart disease is a leading cause of AR in developing countries. Paroxetine
(parox), a selective serotonin reuptake inhibitor, injected subcutaneously improved the systolic
function. This effect can either be due to central or peripheral action. In order to study the effects
separately, we studied the cardiovascular parameters of AR rats that received parox
intracerebroventricular (ICV). Male Wistar rats (250-280g) underwent through sham or AR surgery.
After 4 weeks, stainless steel cannula was implanted into the lateral ventricle (LV). Parox (10
ug/1 ul) or saline were injected every day for two weeks. Morfofunctional analysis was made by
an echocardiogram (ECHO) exam. The rats received a catheter into the femoral artery (to measure
directly arterial pressure) and into the jugular vein (for drug administration). Autonomic
modulation was evaluated after simultaneous blockade with atenolol (8 mg/kg) and atropine (3
mg/kg). Our preliminary results showed no changes in the ECHO variables, neither for
hemodynamics parameters, nor autonomic parameters. Although, parox into the VL seems to
increase heart rate (AR+parox: 38,8 ± 14,9 vs AR+saline: 0,2 ± 10,8 bpm). Therefore, parox
subcutanesouly might have a direct effect on the heart, that did not apper when parox is injected
directly into the LV.
04.064 - ROLE OF CAROTID BODY AND FOREBRAIN IN THE SYMPATHETIC AND RESPIRATORY
RESPONSES INDUCED BY HYPEROSMOLALITY
ELAINE FERNANDA DA SILVA1; MIRIAN BASSI2; JOSÉ VANDERLEI MENANI2; DANIEL BRESEGHELLO
ZOCCAL2; GUSTAVO RODRIGUES PEDRINO1; DEBORA SIMÕES ALMEIDA COLOMBARI2; EDUARDO
COLOMBARI2
1.UNIVERSIDADE FEDERAL DE GOIÁS, GOIANIA, GO, BRASIL; 2.UNIVERSIDADE ESTADUAL DE SÃO
PAULO, ARARAQUARA, SP, BRASIL.
Carotid body and forebrain areas are activated by hyperosmolality. In the present study we
investigated the involvement of the carotid body and forebrain in the changes of thoracic
sympathetic (tSNA) and phrenic (PNA) nerve activities induced by hypertonic saline (HS) infusions
in arterially-perfused in situ preparations of juvenile male rats (60-100 g, n = 5-6/group). Intra-
04.068 - ROLE OF THE MEDIAN PREOPTIC NUCLEUS (MNPO) ON CARDIOVASCULAR AND
AUTONOMIC RESPONSES INDUCED BY HYPERTONIC SALINE IN HEMORRHAGIC SHOCK
LARA MARQUES NAVES; ALINE ANDRADE MOURAO; GUSTAVO RODRIGUES PEDRINO
The present study evaluates the role of MnPO in the autonomic and cardiovascular responses to
hypertonic saline infusion (HSI) in animals submitted to hemorrhagic shock (HS). Male Wistar rats
(280–300 g) were anesthetized and instrumented to mean arterial pressure (MAP), heart rate (HR)
and renal sympathetic nerve activity (RSNA) recordings. Muscimol (4 mM; 100 nL; Experimental
group) or saline (0.15 M; 100 nL; Sham group) nanoinjections in MnPO was performed after 10 min
from the start of HS. 20 min after induction of HS was conducted the sodium overload (3M NaCl;
1.8 ml/kg, iv). Similar hypotension was observed in the experimental and Sham groups during HS
(111.4 ± 3.5 to 62.1 ± 0.5 vs. 99.5 ± 8.6 to 60.4 ± 0.4 mmHg, after 10 min of HS; respectively).
HSI did not promote restoration of MAP to baseline levels in the animals with pharmacological
inhibition of the MnPO (68.8 ± 3.8 mmHg, 20 min after HSI) when compared to the Sham (98.3 ±
5.1 mmHg, 20 min after HSI). The sodium overload promoted renal sympathoinhibition in Sham (37.5 ± 3.7%, 20 min after HSI). Differently, in experimental group the HSI induced high renal
sympathetic response (50.8 ± 10.3 %, 20 min after HSI). The results demonstrate that MnPO is
directly involved in the regulation of cardiovascular and autonomic responses to HSI in HS,
however further investigations are necessary.
04.069 - EFFECTS OF SECONDHAND CIGARETTE SMOKE ON AUTONOMIC CARDIOCIRCULATORY
CONTROL IN CONSCIOUS RATS
NATHALIA RODRIGUES BETTINI1; ANDRESSA CUNHA DIAS1; HILDEMBERG AGOSTINHO ROCHA DE
SANTIAGO2; MARINA DE TOLEDO DURAND1
1.UNIVERSIDADE DE RIBEIRÃO PRETO, RIBEIRAO PRETO, SP, BRASIL; 2.UNIVERSIDADE DE SÃO
PAULO, RIBEIRAO PRETO, SP, BRASIL.
We investigated the effect of secondhand cigarette smoke exposure on basal cardiovascular
parameters, baroreflex function and cardiac autonomic control in conscious rats. Rats were
exposed to environmental air or cigarette smoke twice a day (1 cigarette/animal/day) during 30
days. At the end, rats were anesthetized and femoral artery and vein were catheterized. After 24
hours, basal arterial pressure (AP, mmHg) and heart rate (HR, bpm) were recorded during 30
minutes in conscious rats to assess spontaneous baroreflex sensitivity and HR and AP variabilities.
Phenylephrine and sodium nitroprusside, followed by methylatropine and propranolol (or vice
versa) were administrated for baroreflex evaluation and estimation of cardiac parasympathetic
and sympathetic tone, respectively. Rats exposed to cigarette did not show alterations in basal
AP and HR, spontaneous baroreflex sensitivity and HR and AP variabilities. Cigarette exposure
decreased the reflex bradycardia response to phenylephrine (-55±10 vs. -91±9). The hypotensive
response induced by sodium nitroprusside was blunted in rats exposed to cigarettes (-20±1 vs. -
30±2), but the reflex tachycardia was not affected. Propranolol produced a larger HR decrease in
exposed rats (-28±4 vs. -16±2). Thus, the results show that although cigarette exposure did not
affect basal cardiovascular parameters, it impaired the hypontesive and the reflex tachycardia
responses to vasoactive drugs and increased cardiac sympathetic activation in rats.
04.070 - INCREASED RESTING TENSION ENHANCES PHENYLEPHRINE-INDUCED CONTRACTION
BUT IT DOES NOT MODIFY ACETYLCHOLINE-INDUCED VASODILATION IN RENAL HYPERTENSIVE
(2K-1C) RAT AORTA
BRUNO RODRIGUES SILVA; MARCELLA DARUGE GRANDO; LUSIANE MARIA BENDHACK
The role of mechanical stress for contraction is still unclear in hypertensive animals. This study
aimed to evaluate the role of the resting tension of 1.5 g and 3.0 g on the contraction induced by
the α1-adrenoceptor agonist phenylephrine (PE) and the vasodilation induced by acetylcholine
(ACh) in intact-endothelium aorta (E+) isolated from 2K-1C as compared to normotensive rat aorta
(2K). Concentration-effect curves for PE and ACh were constructed in E+ under resting tension of
1.5 g or 3.0 g. The potency (pD2) and efficacy (ME) of PE and ACh were evaluated. This study was
approved by the Ethics Committee of the University of São Paulo (156/2009). ME induced by PE at
resting tension of 1.5 g was lower in 2K-1C (1.2±0.2g, n=7; p<0.001) than in 2K (2.2±0.1g;
pD2:7.44±0.03; n=5), which was normalized by incubation for 30 min with the NOS inhibitor (LNAME, 100 μM). Under resting tension of 3.0 g PE-induced contraction was similar in 2K-1C and 2K
aorta. Despite of the impaired vasodilation induced by ACh in 2K-1C (ME: 81.4 ± 4.7%; n=7;
p<0.001) as compared to 2K (ME: 100.5 ± 2.4%; n=5), it was not modified at 1.5 and 3.0 g in both
2K or 2K-1C groups. Our results indicate that the anti-contractile effect induced by PE in 2K-1C
(E+) aorta under resting tension of 1.5 g is abolished by the increased tension to 3.0 g. However,
it does not modify the endothelium-dependent vasodilation.
04.071 - PROPRANOLOL TREATMENT IMPROVES INSULIN-VASODILATOR SIGNALING PATHWAY
VIA AKT/ENOS ACTIVATION IN AORTA OF OBESE MICE
NATHALIA SANTOS DA SILVA; DANIELE MENDES GUIZONI; CAMILA LUBACZEUSKI; JUNIA CAROLINA
DOS SANTOS-SILVA; EVERARDO MAGALHÃES CARNEIRO; ANA PAULA DAVEL
Obesity is a risk factor for type II diabetes and cardiovascular diseases. Physiologically, insulin
activates PI3-kinase and protein kinase B (Akt), which stimulates endothelial nitric oxide (NO)
synthase (eNOS) leading to NO production and vasodilatation. However, hyperinsulinemic state
causes endothelial dysfunction, extracellular signal-regulated kinase (ERK) activation, and
increases sympathetic tone to cardiovascular system. Considering that β-adrenoceptor (β-AR)
hyperactivation induces ERK activity and reduces NO bioavailability, we hypothesized that
blocking β-AR hyperactivation would restore endothelial function by improving insulin-vasodilator
signaling in obese mice. 8-week-old male C57BL6 mice were fed with high fat (HF) or chow diet
and were concomitantly treated or not with propranolol (PR, 10 mg/kg/day) for 8 weeks. HF
increased body weight and epididymal fat; PR treatment did not affect it. Relaxation to
acetylcholine (ACh) and to insulin was impaired in aortic rings of obese mice, while relaxation to
NO donor was not changed. Phosphorylation (p) of Akt and eNOS was decreased in aorta of obese
mice; in the opposing way, pERK/ERK was increased. Aorta of PR-treated obese mice displayed
improved ACh- and insulin-induced relaxation, as well normal pAkt, peNOS, and pERK expression.
Thus, enhancing insulin-vasodilator signaling pathway via Akt/eNOS and by reducing ERK
activation, PR treatment seems to be beneficial to vascular dysfunction in obesity.
04.072 - BETA-ADRENERGIC STIMULATION RESPONSES IS ALTERED IN ANESTHETIZED RATS
SUBMITTED TO PILOCARPINE MODEL OF EPILEPSY
KARINA PEREIRA GOMES; ALINE ANDRADE MOURAO; MARIELLY DA SILVA; GUSTAVO RODRIGUES
PEDRINO; CARLOS HENRIQUE DE CASTRO; DANIELLE ALVES IANZER; CARLOS HENRIQUE XAVIER;
DIEGO BASILE COLUGNATI
UNIVERSIDADE FEDERAL DE GOIÁS, GOIÂNIA, GO, BRASIL.
Heart rate variability (HRV) impairment and cardiac changes has been associated with epilepsy
and may explain sudden unexpected death in epilepsy. In these sense we evaluated the HRV, renal
sympathetic activity (RSNA), mean arterial pressure (MAP), systolic pressure, diastolic pressure
and heart rate. Male Wistar rats (240-280g) were submitted to pilocarpine model of epilepsy
(N=10). To epilepsy group (EP) a period of 60 days was expected after the first spontaneous
recurrent seizure. No-status epilepticus animals were used as controls (CN) (N=5). The animals
were anesthetized with halothane (2% in O2 100%) and urethane (1,2 g/kg, iv), followed by
tracheotomy and cannulation into femoral vein and artery. The right renal nerve was isolated and
the noise level of the RSNA recording system was determined after administration of
hexamethonium (30 mg/kg, iv). The electrocardiogram was recorded by means of needle
electrodes placed subcutaneously on the limbs. Phenylephrine (50 μg/kg), sodium nitroprusside
(10 μg/kg) and isoproterenol (ISO) (10 μg/kg) were randomly administered intravenously. The
hypotensive response elicited by ISO was lower in EP (MAP -38,98±2,80%) when compared with CN
(MAP -51,41±2,49%). Furthermore, while RSNA increases in CN (RSNA 12,16±2,71%), there was no
response after ISO (RSNA -0,80±3,82%) in EP. Our results suggested that sympathetic modulatory
alterations of rats with epilepsy are associated with beta-adrenergic mechanisms.
THEME 05 - GASTROINTESTINAL PHYSIOLOGY
05.005 - ROLE OF THE CHOLINERGIC SYSTEM IN THE REMODELING OF THE SMALL INTESTINE
CAUSED BY INTESTINAL MUCOSITIS INDUCED BY 5-FLOUROURACIL
RICARDO DE FREITAS LIMA1; TIAGO SANTOS MENDES1; PEDRO ALMIR FEITOSA MORAIS1; MARCOS
AURÉLIO DE SOUSA LIMA2; PEDRO MARCOS GOMES SOARES1
1.UNIVERSIDADE FEDERAL DO CEARÁ, FORTALEZA, CE, BRASIL; 2.UNIVERSIDADE ESTADUAL DO
CEARÁ, FORTALEZA, CE, BRASIL.
5-Flourouracil (5-FU) is the most used drug in the treatment of cancer colon, being responsible
for many side effects such as intestinal mucositis (MI). During MI peak inflammatory process, ileum
has a reduced response to contractile agonists and increased sensitivity to these agonists in the
post inflammatory period. Our objective was to evaluate the inflammatory aspects and function
of intestinal mucositis induced by 5-FU in mice. Swiss male mice (25-30g) were treated with 5-FU
(450 mg/Kg, ip) and control group received saline (Sal). Animals were sacrificed 3th or 15th days
post application in the 5-FU group and a sample of ileum was collected for assessment of
acethylcholinesterase activity (AChE), MPO activity (U/mg tissue) and contractile response. To
determine the contractility, an isometric force transducer was connected to a system of acquiring
data. Statistical analysis (tests ANOVA followed by Bonferroni and p<0.05). (CEPA: Protocol
74/14). Results: 5-FU induced an increase on the MPO (Sal: 3,24 ± 0,30; 5-FU: 17,92 ± 2,02; U/mg
tissue) and AChE activity (Sal: 971,3 ± 210,9; 5-FU: 376,4 ± 70,98 nM/µg protein/min.) in the 3th
post 5-FU. At 15th days post application (post inflammatory period) the MPO values returned to
baseline levels and AChE activity was still reduced, there was an increase in contractile response.
Conclusion: Intestinal mucositis induced by 5-FU reduced the AChE activity in the inflammatory
and post inflammatory period .
05.006 - UPPER GASTROINTESTINAL INFLAMMATION IS RELATED TO CRURAL DIAPHRAGM
MUSCLE ATROPHY
MARCIA NETTO MAGALHAES ALVES1; MÔNICA COELHO ANDRADE1; JESSICA RAQUEL ALMEIDA
LEANDRO1; RENAN OLIVEIRA SILVA1; RUDY DIAVILA BINGANA1; JULIETE VAZ FERREIRA1; RICARDO DE
FREITAS LIMA1; PATRICIA CHAKUR BRUM2; ARMÊNIO AGUIAR DOS SANTOS1; MIGUEL ÂNGELO NOBRE
E SOUZA1
1.UFC, FORTALEZA, CE, BRASIL; 2.USP, SAO PAULO, SP, BRASIL.
Crural diaphragm (CD) acts as an extrinsic sphincter on esophagogastric junction and is a key
component of the antireflux barrier. We showed that CD is narrower in gastroesophageal reflux
disease (GERD) and this correlates with heartburns episodes (Nobre e Souza et al, 2013). Then,
we began to obtain CD biopsies from GERD patients to assess if ubiquitin proteasome activity
(UPA) in CD muscle is related with faster proteolysis. In this work, we compared the proteasome
activity of CD and of rectus abdominis (RA) in an animal model of a gastrointestinal ulcer disease,
induced by naproxen. Male Wistar rats were pre treated with 0.5% carboxymethylcellulose (control
25
group) for 2 days. After 1 h, naproxen (80 mg/kg, p.o.) was administered (treated group). Rats
were killed on day two, 4 h after naproxen treatment for biopsies of CD and rectus abdominis
muscle (RA). UPA was done following fluorescent product for up to 90 min with a highly specific
inhibitor of chymotrypsin-like proteasome activity, and differences between the two rates were
considered as 26S PA. Data are as mean ± SEM. Besides inducing severe macroscopic gastric and
intestinal lesions, the rat ulcer model increased UPA (103.μF.min-1.mg-1) in CD of treated group
as compared to control group (6926 ± 486.1 v 9085 ± 591, p<0.05) while in RA it remained unaltered
(8570 ± 640.2 v 8790 ± 640.2). We conclude that experimental ulcer alters the UPA activation in
CD muscle, which may be associated with muscular atrophy.
05.007 - EFFECTS OF THE OPIOID RECEPTOR ANTAGONIST ON SALIVARY FLOW REDUCTION
PROMOTED BY CGP DIPEPTIDE IN MICE
IGOR SANTANA DE MELO 1; NÁVYLLA CANDEIA DE MEDEIROS1; JAMYLLE NUNES DE SOUZA FERRO1;
POLLIANE MARIA CAVALCANTE-ARAÚJO1; TALES LYRA DE OLIVEIRA1; CÁSSIO ERÁCLITO ALVES DOS
SANTOS1; EMILIANO DE OLIVEIRA BARRETO1; ROBINSON SABINO-SILVA2
1.UNIVERSIDADE FEDERAL DE ALAGOAS, MACEIO, AL, BRASIL; 2.UNIVERSIDADE FEDERAL DE
UBERLÂNDIA, UBERLÂNDIA, AL, BRASIL.
We evaluated the effects of cyclo-glycine-proline (CGP) and morphine (MOR) in salivary flow and
composition. Male Swiss mice were treated with saline (10 mL/kg, ip), MOR (17.5 µmol/kg; 10
mL/kg, ip) e CGP (1 µmol/kg; 10 mL/kg, ip) and anesthetized with ketamine (35 mg/kg, ip) and
xylazine (5 mg/kg, ip). Similarly, other groups received naloxone (5 mg/kg, ip) 15 minutes before
saline, MOR or CGP administration. Stimulated saliva was collected after pilocarpine
administration (2 mg/kg, ip). The inorganic constituents of saliva were analyzed by X-ray
spectroscopy energy dispersive. The submandibular gland was stained with hematoxylin-eosin and
was also analyzed acetylcholinesterase (AChE) activity by colorimetric assay. The CGP reduced
salivary secretion (p<0.05) compared to control. The activity of AChE and salivary composition of
potassium, chloride, sodium and sulfur ions were not changed by acute treatment with MOR and
CGP. However, only MOR promoted the increase of distance between acinar and ductal cells in
the parenchyma of the submandibular gland. Naloxone increased salivary flow stimulated by
pilocarpine (p<0,005). Therefore, similar to MOR, the CGP reduces salivary flow, but only MOR
promotes morphological changes in the submandibular gland, which indicates that CGP can be less
aggressive when used in long term. In addition, naloxone reduces the inhibitory effect of CGP, as
well as MOR. These data suggest that CGP exert their effects via opioid pathways.
05.008 - ACUTE EXERCISE ACCELERATES THE GASTRIC EMPTYING OF SOLIDS IN AWAKE RATS
RÕMMULO CELLY LIMA SIQUEIRA1; ANA KAROLINA MARTINS CAVALCANTE2; MARCIA NETTO
MAGALHAES ALVES2; ANTONIO KLINGEM LEITE DE FREITAS2; MOISES TOLENTINO BENTO-SILVA3;
ARMÊNIO AGUIAR DOS SANTOS2
1.UNIVERSIDADE FEDERAL DO CEARÁ - UFC, FORTALEZA, CE, BRASIL; 2.INSTITUTO SUPERIOR DE
TEOLOGIA APLICADA, FORTALEZA, CE, BRASIL; 3.UNIVERSIDADE FEDERAL DO PIAUÍ, PIAUI, PI,
BRASIL.
Introduction: We have shown that acute exercise (AEx) delays the gastric emptying (GE) of liquid
in awake rats (J Appl Physiol 116 1131, 2014). Now, we decided to assess the effect of high
intensity swimming on the GE of solids. Methods: Male Wistar rats (200-250g, n=5) were trained
during 5 days to be restricted into a PVC tube. After 16h of fasting, they were submitted to
sedentary (SED) and AEx protocols, one week later. AEx session consisted of swimming against an
extra load (5% of the body weight) during 15 min into a cylindrical tank (60cm diameter, 50cm
depth, 30 ± 1°C). A peripheral blood sample (25μl) was obtained for Lactate [LAC] analysis. GE of
solids was indirectly evaluated by the respiratory method. Next, the rats were restricted into the
tube and received the test meal (1 g) containing 1μg - octanoic acid composed of 13C. Air samples
were postprandially collected at times: 0, 15, 30, 60, 75, 90, 105, 120, 150, 180, 210 and 240 min
and evaluated by an automatic analyzer isotope (IRIS 2) that calculates the T lag and T1/2. Results:
In comparison with SED protocol, AEx increased (p <0.05) LAC levels (4.0 ± 0.4 vs. 6.9 ± 0.8
mmol/dL). In comparison with SED protocol, AEx decreased (p <0.05) the T lag (31.4 ± 6.8 min. vs
24.8 ± 4.2 min.) and T1/2 (62, 6 ± 14.4 vs. 32.2 ± 5.7 min.) values. Conclusion: Besides inducing
lactic acidosis, AEx decreased the Tlag and T1/2, indicating an acceleration of GE of solids.
THEME 06 - CELULAR PHYSIOLOGY
06.004 - A RESTRIÇÃO DE SONO PARADOXAL NÃO ALTERA A VIA DE SÍNTESE DE PROTEÍNAS NO
MÚSCULO PLANTAR DE RATOS
GUILHERME REIS DIAS; SARA QUAGLIA DE CAMPOS GIAMPÁ; HELTON DE SÁ SOUZA; MARCOS
MÔNICO-NETO; KIL SUN LEE; SERGIO TUFIK; HANNA KAREN MOREIRA ANTUNES
UNIVERSIDADE FEDERAL DE SÃO PAULO, SANTOS, SP, BRASIL.
Introdução: Estudos demonstram que a privação de sono paradoxal por 96 horas em ratos causa
atrofia da musculatura esquelética, contudo, o efeito da restrição crônica de sono na manutenção
deste tecido não é conhecido.
Objetivo: Avaliar os efeitos da restrição de sono paradoxal na via de síntese proteica no músculo
plantar de ratos.
Métodos: A pesquisa foi aprovada pelo CEP (#0764/10). Foram utilizados 20 ratos machos Wistar,
com 3 meses de idade, distribuídos em dois grupos: controle (CTRL, n=10) e restrição de sono
paradoxal (RS, n=10). O protocolo utilizado foi o método das plataformas múltiplas modificado,
mantidos por 21 dias consecutivos. O protocolo foi iniciado às 16 horas do dia e finalizado às 10
horas do dia seguinte. O intervalo das 10h às 16h correspondeu à janela de sono diária dos animais.
Após o 21º dia os animais foram eutanasiados para extração do músculo plantar. Este foi pesado
e posteriormente homogeneizado para análise molecular das proteínas Akt, mTOR, p70S6K e 4EBP1 e suas porções fosforiladas. Os dados estatísticos foram tratados com teste T independente.
O valor de significância adotado foi de p<0,05.
Resultados: A massa do músculo plantar reduziu significativamente (p<0,001) no grupo RS quando
comparado ao grupo CTRL. Ao analisar a via de síntese proteica, não foram observadas diferenças
significativas entre os grupos.
Conclusão: A restrição de sono paradoxal por 21 dias reduziu a massa do músculo plantar dos ratos
mas não houve alteração na via de síntese de proteínas.
06.005 - DEXAMETHASONE EFFECT ON CLOCK GENES AND XPA EXPRESSION IN MURINE MELANA AND B16-F10 CELLS
MARISTELA DE OLIVEIRA POLETINI1; LEONARDO VINÍCIUS MONTEIRO DE ASSIS2; MARIA NATHÁLIA
NATHÁLIA MORAES2; ANA MARIA DE LAURO CASTRUCCI2
1.FEDERAL UNIVERSITY OF MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.UNIVERSITY OF SAO
Understanding the molecular processes that take place in cancer is crucial for creating new
therapies. Clock genes are of interest since they control a variety of cellular processes; however
their role in melanocytes is not well understood. We analyzed Per1, Per2, Bmal1 and Xpa
(Xeroderma pigmentosum complementation group A, a protein essential for DNA repair)
expression in murine, Melan-A and B16-F10, cells after a 2-hour pulse of 10-7 M dexamethasone
(DEX). Our data show that Per1 and Per2 displayed a temporal variation of expression in Melan-A
cells kept in constant dark (DD); this oscillatory profile was unaffected by DEX treatment. In B16F10 cells there was no oscillation of Per1 and Per2 expression in DD, but an acute increase of
transcripts was seen 6 hours after DEX treatment. Bmal1 expression was constant throughout the
day in both cell types, in either control or DEX-treated group. Xpa did not vary along 24 h in B16F10 cells, in both control and DEX-treated groups; however in Melan-A cells, Xpa exhibited a
circadian oscillation which peaked at ZT18 in control cells, and at ZT24 in DEX-treated cells. We
may hypothesize that: a) Per1, Per2 and Xpa oscillatory patterns seen in Melan-A cells are lost in
B16-F10 cells as a result of the carcinogenic process; b) Per1 and Per2 expressions are acutely
stimulated by DEX in B16-F10 cells probably because in DD these cells, unlike Melan-A cells, are
not synchronized; c) DEX is able to phase delay the oscillatory expression of Xpa in Melan-A cells.
06.006 - TRPA1 TRANSCRIPTION IS ALTERED IN THE ABSENCE OF TRPV1: CLOCK MOLECULAR
MACHINERY REGULATION IN MUS MUSCULUS LIVER
MARIA NATHÁLIA NATHÁLIA MORAES 1; NATHANA MEZZALIRA1; MICHAEL MENAKER2; ALI GULER2; ANA
MARIA DE LAURO CASTRUCCI1
1.UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSITY OF VIRGINIA,
CHARLOTTESVILLE, ESTADOS UNIDOS.
Daily cycles of light and of temperature are major environmental signals for organisms. Recently
the interaction of opsins with TRP channels was demonstrated in photo- as well as thermoresponses (Shen, WL Science 331, 1333, 2011). We hypothesized that temperature cycles signal
mammalian peripheral clocks via TRP channel activation, and that this signal synchronizes
peripheral clocks. We investigated the function of TRPV1 channels by assessing clock genes
expression in the liver of wild type (WT) and TrpV1 knockout (TrpV1-/-) mice in constant darkness
(DD) or light-dark cycles (LD). TrpV1-/- mice displayed normal locomotor activity and body
temperature, suggesting that light information from the retina to the central clock (SCN), and its
processing in the suprachiasmatic nuclei are not altered in the absence of TRPV1 channel. In the
liver, Per1 and Clock transcripts showed temporal variation in DD, Per2 oscillated in LD, and Bmal1
oscillated in both conditions. No differences were observed in clock gene expression in the liver
of TrpV1 -/- as compared to WT animals in both LD and DD. In WT animals, liver TrpV1 oscillated
along 24 hours, peaking in the scotophase, and TrpA1 showed constant temporal expression; in
TrpV1-/- animals, TrpA1 oscillated with a peak in the photo-phase. The differential expression of
TrpA1 in TrpV1-/- and WT mice suggests a compensatory expression of TRPA1 channel in the
absence of TRPV1, and a putative role for these channels in the regulation of liver clock
machinery.
THEME 07 - COMPARATIVE PHYSIOLOGY
07.006 - EFFECTS OF ACIDIFICATION OCEAN UNDER THE PHYSIOLOGY SHRIMP SEVEN BEARDS,
XIPHOPENAEUS KROYERI, SUMMER AND WINTER
PAULO VICTOR LEME MANTOAN; ANDRESSA CRISTINA RAMAGLIA DA MOTA; ALESSANDRA DA SILVA
AUGUSTO
UNESP - CLP, SAO VICENTE, SP, BRASIL.
Evaluated the synergistic effect of hypercapnia and seasons on metabolism, excretion of
ammonia, energy substrate and index hepatosomatic X. kroyeri. They were kept in normocapnia
(pH 8.0) or hypercapnia (pH 7.3) for three days, according to the season. Acidification of the
water made by CO2 bubbles. Metabolism measured by oxygen consumption; ammonia excretion
by colorimetry. The hepatosomatic with getting the dry mass of the organ and the animal and the
energy substrate used (protein, carbohydrate or lipid) by atomic ratio. The reduction in pH,
affects metabolism, excretion of ammonia and the hepatosomatic in summer. In the summer 30%
reductions occur in the metabolism of 50.4 ± 5.6 to 48.8 ± 4.7 and a 40% ammonia excretion from
7.5 ± 0.3 to 4.5 ± 0.6 pH 7.3. The synergy of high temperature and low pH may have affected the
reactions by reducing the metabolism. Since the reduction in excretion of ammonia was
accompanied by a reduction in metabolism, it is possible that the animals spend energy reduced
activities that hypercapnia during stress in summer and thus reduced the excretion of subsequent
catabolism ammonia. The hepatosomatic increased when exposed to pH 7.3 during the summer,
suggesting the suppression of activities that require its use. Regardless of pH and the seasons,
animals use protein as an energy substrate. The changes observed in X. kroyeri kept hypercapnia
may result in reduction in growth rate or reproduction.
07.007 - EVALUATION OF THE EFFECT FUTURE SCENARIOS TO OCEAN ACIDIFICATION IN THE
OSMOREGULATORY MECHANISMS SHRIMP SEVEN BEARDS (XIPHOPENAEUS KROYERI)
ANDRESSA CRISTINA RAMAGLIA DA MOTA; PAULO VICTOR LEME MANTOAN; TAYNA FIGEIREDO
STREFEZZA; ALESSANDRA DA SILVA AUGUSTO
UNESP- CAMPUS DO LITORAL PAULISTA, SAO VICENTE, SP, BRASIL.
The increase in atmospheric CO2 concentration has caused acidification in the ocean waters.
Models suggest a future scenario in which the pH of the oceans could be reduced by up to 0.7 unit
until 2250, generating negative impacts on vulnerable organisms. Studies have shown that
acidification can affect several physiological mechanisms including those related to
osmoregulation. This study evaluated the effect of ocean acidification on osmoregulatory
processes shrimp X. kroyeri, species widely distributed in Brazil and one the main fishery resources
of the São Paulo Coast. The osmoregulatory mechanisms have been investigated in animals kept
for three days in hypercapnic conditions (pH: 7.3) or normocapnia (pH: 8.0) and the salinity of 20,
25, 30 and 35. We evaluated the osmolality of the hemolymph and the degree of hydration of the
muscle tissue. In both pH conditions, the animals remained strict osmoconformers with variations
in the hemolymph osmolality between 610.83 ± 23,497 (20 S) and 999.143 ± 37,751mOsm / kg
water (S 35). The degree of hydration of the muscle tissue remained unchanged in all salinities
and pH values evaluated at about 78% ± 3%. The results show that the laboratory simulation of
future scenarios ocean acidification does not influence the degree of hydration and the
osmoregulatory standard species, one osmoconformador, in the salinities evaluated here.
07.008 - THE EFFECT OF OCEAN ACIDIFICATION ON PHYSIOLOGY SHRIMP SEVEN BEARDS,
XIPHOPENAEUS KROYERI (DECAPODA, PENAEIDAE)
ANDRESSA CRISTINA RAMAGLIA DA MOTA; PAULO VICTOR LEME MANTOAN; ALESSANDRA DA SILVA
AUGUSTO
UNESP- CAMPUS DO LITORAL PAULISTA, SAO VICENTE, SP, BRASIL.
Ocean acidification may reduce aquatic biodiversity through changes in the biology of the species.
The effect of acidification was evaluated on aspects of physiology (metabolism, excretion
ammonia, energy substrate used and hepatosomatic index) of the X. kroyeri marine shrimp, a
widely distributed specie in Brazil and one of the main fishery resources in the state of São Paulo.
The animals were kept for three days under conditions of normocapnia (pH: 8.0) or hypercapnia
(pH: 7.3) in the salinities of 20, 25, 30 and 35. Metabolism was measured by the oxygen
consumption and the ammonia excretion by colorimetry. Hepatosomatic index was calculated by
the ratio of the dry weight from the body and the total weight of the animal. Oxidized energy
substrate (proteins, carbohydrates or lipids) was calculated using the atomic relation between the
consumed oxygen and nitrogen excreted. Results show that ocean acidification may cause
metabolism reductions (up to 64%, from 2.03 ± 0.16 to 0.73 ± 0.09 20 S), excretion of ammonia
(75%; 1.32 ± 0.19 to 0.33 ± 0.06 35 S) and hepatosomatic index (about 80%, from 7.62 ± 1.76 to
1.55 ± 0.33 25 S) regardless of salinity. Oxidized energy substrate in both pHs remains unchanged,
predominantly proteins. Metabolism reduction could cause changes in the pattern of activity,
growth and reproduction. The high reduction in hepatosomatic could especially compromise
reproduction through the gonadal development
and the yelk formation.
07.009 - EFFECT OF HYDRIC STRESS DURING DEVELOPMENT ON METABOLIC RATE IN IMAGO OF
LEPTODACTYLUS FUSCUS
DIEGO PIMENTEL VENTURELLI; PEDRO HENRIQUE TREVIZAN BAU; WILFRIED KLEIN
Several species of frogs breed in temporary pools maintained exclusively by rainfall. These pools
easily dry out causing high mortality of eggs or tadpoles. However, the tadpoles of some species,
such as Leptodactylus fuscus, can survive up to five days in pools that are drying out, needing only
a humid substrate for development. It is not known which possible damages dehydration stress
causes in tadpoles during their ontogeny. The goal of this study was to analyze the effect of hydric
stress during development on metabolic rate in imago of L. fuscus. Treatment (T): tadpoles where
either transferred to containers with absorbent tissue paper wetted with 4 mL of water and
maintained for 12, 24 and 72 hours (h). Control (C): tadpoles were maintained in containers with
100 mL water for 12, 24 and 72 hours (h). Afterwards, all tadpoles were kept in containers with
100 mL water until metamorphoses. Gas exchange of imagos was measured using closed
respirometry at 25°C. No significant difference in oxygen consumption comparing T and C groups
was detected in all intervalls: 12h C (1,14±0,77 µLO2.min-1.g-1) T (1,40±0,30 µLO2.min-1.g-1),
24h C (1,23±0,29 µLO2.min-1.g-1) T (2,55±2,50 µLO2.min-1.g-1), 72h C (2,32±0,48 µLO2.min-1.g1) T (2,92±0,95 µLO2.min-1.g-1). Thus, hydric stress during development of L. fuscus did not affect
metabolic rate of metamorphosized animals, corroborating the current hypothesis that tadpoles
of L. fuscus show admirable survival capacities out of water.
26
07.010 - THERMOREGULATORY AND NEUROPHYSIOLOGICAL RESPONSES IN INSECTS
GUILHERME GOMES1; IVAN CESAR DESUÓ2; CLAUDIO JOSÉ VON ZUBEN 2; DENIS OTÁVIO VIEIRA DE
ANDRADE2; ROLAND KÖBERLE1
1.USP - DEPARTAMENTO DE FÍSICA E CIÊNCIA INTERDISCIPLINAR - IFSC, SÃO CARLOS, SP, BRASIL;
2.UNESP - DEPARTAMENTO DE ZOOLOGIA - IB, RIO CLARO, SP, BRASIL.
Specific characteristics (anatomical, behavioral and physiological) are key to the great diversity,
reproductive success and adaptation of a group of animals in the environment. Among the animals,
the insects are the most abundant taxon, greater diversity and more adapted in the terrestrial
environment. In general, insects are considered ectothermic, and depend on external sources of
heat and/or behaviors to control body temperature. Currently, the use of new technologies can
facilitating and enabling the description of unpublished physiological processes. We utilized
species representing of orders Diptera (Calliphoridae) and Hymenoptera (Epiponini) because the
importance of these orders to humans and complexity of behavior that can display. The objective
of the studies was to evaluate the production and/or heat exchange during post-embryonic
development, foraging activity and social interactions in different weather conditions. And the
main results were: thermogenesis process of larval aggregates and influence in forensic science;
transfer and heat transport between individuals in the social behavior of animals and stages of
development; heating behavior during early foraging activity; the influence of the structure on
the insulation nests and hatching eggs; besides cooling behavior by saliva drop during specific
conditions of use. These results show a promising future for the understanding of the physiology
of the species, which would assist in different areas of study, such as pest control, forensics,
ecophysiology, biomimetics, among others.
THEME 08. RESPIRATORY PHYSIOLOGY
08.016 - PERIPHERAL ANGIOTENSIN-II IS NOT ASSOCIATED WITH THE CARDIORESPIRATORY
DISORDERS IN RATS SUBMITTED TO POST-WEANING PROTEIN RESTRICTION FOLLOWED BY
REFEEDING
RENATO WILLIAN MARTINS SÁ1; ANDREA SIQUEIRA HAIBARA2; PAULA MAGALHÃES GOMES1; GIOVANA
LOPES AGUIAR1; MARIA LÚCIA PEDROSA1; ANDREIA CARVALHO ALZAMORA1; LISANDRA BRANDINO DE
OLIVEIRA1; LEONARDO MÁXIMO CARDOSO1
1.UNIVERSIDADE FEDERAL DE OURO PRETO, OURO BRANCO, MG, BRASIL; 2.UNIVERSIDADE FEDERAL
We tested the effects of low protein diet followed by refeeding on ventilatory and cardiovascular
responses to chemoreflex activation before and after peripheral angiotensin II blockade with
losartan. Male Fischer rats were divided into control (n=12) and refed (R-PR, n=12) groups after
weaning. R-PR were fed low protein diet (8% as casein) for 35 days and refed normal protein diet
(20%) for 70 days. Control received normal protein diet for 105 days. After cannulation, mean
arterial pressure (MAP), heart rate (HR), respiratory frequency (fR), tidal volume (Vt) and
ventilation (VE) were acquired. Arterial chemoreflex was elicited by KCN (60µg/kg iv). Losartan
was injected iv (20mg/kg). At baseline, R-PR rats presented increased HR and VE (374±8bpm and
1274±126ml/kg/min) compared to control rats (333± 8bpm and 856±39ml/kg/min). MAP, fR and
Vt were not different between the groups. Pressor and bradycardic responses evoked by KCN were
higher in R-PR (47±4mmHg and -75±16bpm) than in controls (22±4mmHg and -21±6bpm). No
difference was found on tachypneic response. Alterations in baseline parameters and responses
to chemoreflex activation in R-PR were preserved after losartan. Data suggest that peripheral
angiotensin II may not be associated with increased HR, VE and cardiovascular responses to
chemoreflex activation in rats submitted to post-weaning protein restriction followed by
refeeding.
08.017 - EFFECTS OF PERINATAL FLUOXETINE ON THE VENTILATORY RESPONSE TO CO2 AND
POTENTIAL MECHANISMS OF ACTION IN LOCUS COERULEUS (LC)
VIVIAN BIANCARDI1; TUCAAUÊ S ALVARES2; LUCIANE HELENA GARGAGLIONI BATALHAO 3; GREGORY
DOUGLAS FUNK2
1.UFSCAR/UNESP, JABOTICABAL, SP, BRASIL; 2.UNIV OF ALBERTA, EDMONTON, CANADA;
3.FCAV/UNESP, JABOTICABAL, SP, BRASIL.
Perinatal exposure of rats to serotonin (5HT) reuptake inhibitors (SSRIs) results in adults with
hyperexcitable LC neurons. Because a significant number of pregnant women take SSRIs and 5HT
is released in the LC, this study aimed to investigate during postnatal development (P0-24) the
effects of perinatal (E15-21) fluoxetine on the ventilatory response to 7% CO2 of rats and to assess
in naïve newborn rats the mechanisms by which 5HT affects the excitability of LC neurons. The
CO2 ventilatory response of fluoxetine-exposed rats was greater than control (P0-2: 31%; P6-8:
28%; P22-24: 16% greater) except in P12-14. Of 150 whole-cell voltage-clamped LC neurons, 101
responded to 5HT (100µM) with an inward current (-30 pA), 18 responded with oscillations (0.4
Hz), and 31 did not respond. 5HT3R agonists had no effect. 5HT2R agonists (PNU22394 & TCB-2,
100µM) evoked 3 responses: an 822% increase in the freq of glutamatergic mEPSCs; an inward
current (-15 pA); and a nitrendipine-sensitive 0.1 Hz oscillation. The 5HT1AR agonist 8-OH-DPAT
(50µM) induced an inward current (-14 pA), while Sumatriptan, a 5HT1ABDR agonist had no effect.
Thus, perinatal SSRI exposure promotes long-term alterations in respiratory chemosensitivity. Part
of this effect may reflect actions of 5HT on LC neurons that include a presynaptic 5HT2-mediated
increase in glutamatergic EPSCs and a postsynaptic facilitation of Ca2+-dependent membrane
oscillations and a 5HT2/5HT1A- inward current.
08.018 - TYROSINE MUTATION IN AAV9 CAPSID IMPROVES GENE TRANSFER TO THE MOUSE LUNG
SABRINA V MARTINI; ADRIANA LOPES DA SILVA; DEBORA PIRES FERREIRA; KARINA GOMES; ORNELLAS
FELIPE; PATRICIA RIEKEN MACEDO ROCCO; HILDA PETRS-SILVA; MARCELO MARCOS MORALES
FEDERAL UNIVERSITY OF RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL.
Adeno-associated viruses (AAV) vectors have become important gene delivery tools for the
treatment of pulmonary diseases. Newly, it was shown that phosphorylation of surface-exposed
tyrosine residues from AAV capsid targets the viral particles for proteasome-mediated
degradation, and mutations of these tyrosine residues lead to highly efficient vector transduction
in vitro and in vivo in different organs. In this study we evaluated the pulmonary transduction
efficacy of tyrosine-mutant AAV9 vector. C57BL/6 mice were randomly assigned into 3 groups:
control group (CTRL) animals submitted to intratracheal instillation of saline, wild-type AAV9
group (WT-AAV9) and tyrosine-mutant Y731F AAV9 group (M-AAV9) that received AAV9 vectors
containing the DNA sequence of green fluorescence protein. Four weeks after instillation, lung
mechanics, morphometry, tissue cellularity and vector transduction were analyzed. Tyrosinemutant AAV9 vectors displayed significantly increased trans-duction efficiency in the lung
compared with their wild-type counterparts. No significant differences were observed in lung
mechanics and morphometry among experimental groups. The number of polymorphonuclear cells
was higher in WT-AAV9 group than in CTRL and M-AAV9 group, suggesting that the administration
of tyrosine-mutant AAV9 vectors was well tolerated. These findings motivate the further
development of viral-based gene therapies for safe and effective delivery of therapeutic genes
for the treatment of respiratory diseases.
08.019 - EFFECTS OF DNA HYPOMETHYLATION DURING POSTNATAL AGE ON VENTILATORY
RESPONSES TO HYPOXIA AND HYPERCAPNIA
MIGUEL FURTADO MENEZES; DANIEL BRESEGHELLO ZOCCAL
SÃO PAULO STATE UNIVERSITY (UNESP), ARARAQUARA, SP, BRASIL.
During first postnatal weeks, the respiratory central pattern generator (rCPG) undergoes a period
of dramatic changes involving modifications in the neuronal excitability and synaptic properties.
Herein, we investigated whether DNA methylation during early post-natal period contributes to
postnatal maturation of rCPG. Male Holtzman rats (P1, n=6-7/group) received either decitabine
(a hypomethylating agent, 1mg/kg) or veichle (control) i.p. injections every 3 days during the 10
days. At 35-day old, the respiratory frequency (fR), tidal volume (VT), ventilation (VE) and
expiratory (EP) and inspiratory (IP) peaks were measured at resting and during hypercapnia (7%
CO2 for 10 min) and hypoxia (7% O2 for 10 min) conditions. Decitabine treatment did not change
baseline respiratory parameters (decitabine, fR: 111±8 cpm, VT: 12.9±1.1 mL/Kg, VE: 1475±251
ml/kg/min, EP: 8.4±0.9 ml/s and IP: 8.4±0.9 ml/s vs. vehicle, fR: 115±4 cpm, VT: 11.7±0.9 mL/Kg,
VE: 1378±168 ml/kg/min, EP: 7.9±0.5 ml/s and IP: 8.5±0.8 ml/s) During hypercapnia, the
tachypneic response was smaller in decitabine group than in controls (fR: 137±13 vs. 169±9 cpm),
with no differences in VT, VE, EP and IP reflex responses. During hypoxia, the group that received
decitabine injections showed a greater increase in ventilation (3448±282 vs. 2782±155
ml/kg/min), but not in the VT, VE, EP and IP, in comparison to control group. The results suggest
that DNA methylation during post-natal age is an important mechanism that appears to be involved
in the development of chemoreflex control of ventilation.
08.020 - REGULATORY T CELLS AS A POTENTIAL MECHANISM OF ACTION OF ADIPOSE TISSUEDERIVED MESENCHYMAL CELL THERAPY IN EXPERIMENTAL ALLERGIC ASTHMA INDUCED BY
HOUSE DUST MITES
VIVIAN ALVES CASTELO BRANCO DA SILVA1; DEBORA GONÇALVES XISTO1; SORAIA CARVALHO
ABREU1; LIGIA LINS DE CASTRO1; JAMIL ZOLA KITOKO1; FLAVIA REGINA GREIFFO2; RODOLFO DE
PAULA VIEIRA2; PATRICIA RIEKEN MACEDO ROCCO1; PRISCILLA CHRISTINA OLSEN1
1.UFRJ, RIO DE JANEIRO, RJ, BRASIL; 2.UNJ, SÃO PAULO, SP, BRASIL.
Rationale: Asthma is a chronic inflammatory disease that affects both the airways and lung
parenchyma, causing structural changes which accelerate decline in lung function despite inhaled
corticosteroid therapy. Therefore, new strategies that can attenuate airway inflammation and
remodeling are warranted. We sought to investigate whether adipose tissue-derived mesenchymal
stromal cells (AD-MSC) reduce lung inflammation by modulating regulatory cells (e.g., Treg) in a
model of asthma.
Methods: C57BL/6 mice (n=24) were randomly divided into 2 groups. HDM (house dust mite) mice
were challenged 3 times with HDM (100 µg in 50 µL intratracheally) once a week, while controls
(C) received saline (SAL) under the same protocol. HDM and C groups were further subdivided to
receive SAL (50 µL) or AD-MSC (105, i.t.) 3 days after the last challenge. Seven days after the
therapy, animals were euthanized.
Results: HDM-SAL exhibited increased static elastance (28%), alveolar collapse (500%), total
leukocytes (114%) and dendritic cells (50%). In HDM-challenged mice, AD-MSCs reduced static lung
elastance (25%) and alveolar collapse (300%), with no difference in Treg counts.
Conclusion: AD-MSC therapy was not effective in reducing lung inflammation in this HDM-induced
asthma model, despite lung function improvement. Additional studies should be performed to
evaluate further potential mechanisms of action for AD-MSCs.
08.021 - PSEUDOMONAS AERUGINOSA-INDUCED PNEUMONIA IN RATS: EFFECTS ON LUNG AND
SYSTEMIC INFLAMMATION
RAQUEL FERREIRA MAGALHÃES1; CYNTHIA SANTOS SAMARY1; RAQUEL SOUZA SANTOS1; MILENA
VASCONCELLOS DE OLIVEIRA1; JAMIL ZOLA KITOKO1; CARLOS ANDRÉ MANDARINO SILVA2; CAROLINE
LOUREIRO HILDEBRANDT2; CASSIANO FELIPPE GONÇALVES DE ALBUQUERQUE2; VERA LÚCIA
CAPELOZZI3; MARCELO MARCOS MORALES 1; PRISCILLA CHRISTINA OLSEN4; PATRICIA RIEKEN
MACEDO ROCCO1; PEDRO LEME SILVA1
1.IBCCF/UFRJ, RIO DE JANEIRO, RJ, BRASIL; 2.IOC/FIOCRUZ, RIO DE JANEIRO, RJ, BRASIL;
3.DP/USP, SÃO PAULO, SP, BRASIL; 4.FF/UFRJ, RIO DE JANEIRO, RJ, BRASIL.
OBJECTIVE: Pseudomonas aeruginosa is an important pathogen causing a wide range of acute and
chronic infections. We aimed to develop a model of pneumonia induced by P. aeruginosa and
evaluate pulmonary function, as well as lung and systemic inflammation.
METHODS: Wistar rats (n=16) were randomly divided to receive saline (200 µL, CTRL) or P.
aeruginosa (5 x 107 colony-forming units [CFU] in 200 µL, PA) intratracheally. After 24 hours,
respiratory system mechanics, blood-gas exchange, total and differential cell counts in
bronchoalveolar lavage fluid (BALF) and blood, total protein content, and CFUs were analyzed.
RESULTS: PA compared to CTRL animals had higher cell counts in BALF (69.6 vs. 3.9 x 105, p<0.05)
and blood (8.3 vs. 6.2 x 103/mm3, p<0.05), associated with increased neutrophils and
macrophages. In BALF, CFU and protein levels were higher in PA compared to CTRL (172 vs. 0
CFUs, p<0.05, and 1.04 vs. 0.46 µg/mL, p<0.05, respectively). In blood, CFUs were also higher in
PA compared to CTRL (10.5 vs. 0.0 CFUs, p<0.05). PA animals exhibited lower oxygenation, but
no significant differences in respiratory system mechanics.
CONCLUSION: In this rat model of P. aeruginosa pneumonia, increased CFU and lung inflammation
were associated with oxygenation impairment with no significant changes in lung mechanics. This
model of acute pneumonia can be an important tool to investigate new therapeutic strategies.
08.022 - THE BENEFICIAL EFFECTS OF BONE MARROW-DERIVED MONONUCLEAR CELL THERAPY
IN EXPERIMENTAL EMPHYSEMA DEPEND ON ROUTE OF ADMINISTRATION
LUÍSA ALCHAAR DE FARIA1; MARIANA ALVES ANTUNES1; SORAIA CARVALHO ABREU1; NAZARETH DE
NOVAES ROCHA1; VERA LÚCIA CAPELOZZI2; MARCELO MARCOS MORALES1; PATRICIA RIEKEN MACEDO
ROCCO1; FERNANDA FERREIRA CRUZ1
1.UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL; 2.UNIVERSIDADE DE
Rationale: We hypothesized that the beneficial effects of bone marrow-derived mononuclear cells
(BMDMCs) may vary depending on route of administration in a murine model of elastase-induced
emphysema.
Methods: C57BL/6 mice were assigned into 3 groups. Control (C) animals received intratracheal
(IT) saline (50 µL), whereas emphysema (E) mice received porcine pancreatic elastase 1x/week
for 4 weeks at two different doses: 0.1IU and 0.2IU. Animals were further randomized to receive
saline (50 µL) or BMDMC (2x106) intravenously (IV) or IT 3 h after the last saline or elastase. 7 days
after cell administration, animals were euthanized for analysis.
Results: On day 7, E animals had increased static lung elastance, mean linear intercept,
polymorphonuclear and mononuclear cell counts in lung tissue, alveolar hyperinflation and
collapse, TGF- levels, elastolysis, airway fibrosis, cardiac and lung cell apoptosis,
echocardiographic alterations, ultrastructural changes in epithelial and endothelial cells and
extracellular matrix, and lower VEGF and IL-10 levels compared to C (higher with 0.2IU than
0.1IU). BMDMCs improved lung mechanics and reduced inflammatory and morphometric changes,
mainly through the IT route, in both groups, but did not yield echocardiographic improvement.
Conclusion: BMDMCs effectively modulated the inflammatory and remodeling processes, thus
improving lung mechanics, mainly when given IT.
08.023 - DIFFERENT SOURCES OF MESENCHYMAL STEM CELLS YIELD DIVERSE EFFECTS ON LUNG
INFLAMMATION AND REMODELLING IN EXPERIMENTAL ALLERGIC ASTHMA
TAINÁ BATISTA DE OLIVEIRA; VIVIAN ALVES CASTELO BRANCO DA SILVA; MARIANA ALVES ANTUNES;
MARCELO MARCOS MORALES; DEBORA GONÇALVES XISTO; BRUNO LOURENÇO DIAZ; PATRICIA
RIEKEN MACEDO ROCCO; SORAIA CARVALHO ABREU
Rationale: Different sources of mesenchymal stem cells (MSCs) generally meet accepted criteria
for MSC definition, but MSCs can be distinguished by cytokine production and gene expression.
This study aims to investigate the effects of MSCs from different sources on inflammation and
remodeling in an experimental model of asthma.
Methods: 48 C57BL/6 mice were assigned into 8 groups. In the OVA group, mice were sensitized
and challenged with ovalbumin while controls (C) received saline using the same protocol. 24
hours after the last challenge, C and OVA groups were further divided to receive saline (50 µL,
SAL, n=6), bone marrow, adipose MSCs, or lung MSCs (105, BM-MSC, AD-MSC and L-MSC, n=6/each)
intratracheally. One week after treatment, airway responsiveness, lung histology, macrophage
polarization and inflammatory mediators were analyzed.
Results: MSC therapies reduced airway responsiveness, inflammatory cell infiltration, collagen
content and levels of IL (interleukin)-4, IL-13, transforming growth factor-β and vascular
endothelial growth factor, as well as an increase in M2-macrophage polarization compared to OVASAL. However, these decrements were more pronounced after BM-MSC therapy.
Conclusion: MSCs were effective at modulating inflammation; however, lung mechanics and
remodeling showed greater improvement after BM-MSC than AD-MSC and L-MSC administration.
08.024 - BIODEGRADABLE DNA NANOPARTICLES FOR EFFICIENT IN VIVO THYMULIN GENE
DELIVERY IN EXPERIMENTAL ALLERGIC ASTHMA
27
NATALIA GOES BLANCO1; ADRIANA LOPES DA SILVA1; PANAGIOTIS MASTORAKOS2; JUNG SOO SUK2;
JUSTIN HANES2; MARCELO MARCOS MORALES1; PATRICIA RIEKEN MACEDO ROCCO1
1.UFRJ, RIO DE JANEIRO, RJ, BRASIL; 2.JOHNS HOPKINS UNIVERSITY, BALTIMORE, ESTADOS
UNIDOS.
Rationale: Currently available therapies for asthma are often ineffective or, as in the case of
corticosteroids, cause serious side effects when chronically administered. Poly(β-amino esters)
have generated interest as nontoxic, efficient, biodegradable polymer carriers for plasmid DNA
(pDNA). We aimed to investigate whether thymulin gene therapy delivered by a novel
biodegradable nanoparticle platform could be effective in experimental allergic asthma.
Methods: Particles were formulated and characterized. Transfection efficiency was determined
and nanoparticle diffusivity assessed in lung mucus by the multiple-particles tracking method.
Mice were then sensitized and challenged with ovalbumin (OVA). 24 h after the last challenge,
thymulin (100 ug DNA) or saline were intratracheally instilled. After 20 days, animals were
euthanized and lung morphofunction analyzed.
Results: DNA nanoparticles exhibited a near-neutral charge. The hydrodynamic diameter was
45.6±2.6 nm. Particles exhibited significant transfection efficiency and displayed trajectories that
spanned greater distances compared to commonly used particles. In OVA mice, thymulin
nanoparticles were effective in reducing inflammatory cell infiltration and improving static lung
elastance.
Conclusion: In this model of allergic asthma, thymulin gene therapy delivered by a novel platform
effectively reduced lung inflammation.
08.025 - EFFECTS OF PROPOFOL AND DEXMEDETOMIDINE ON LUNG MORPHOFUNCTION AND
BIOLOGICAL MARKERS IN EXPERIMENTAL OBESITY
MARIANA COELHO DA SILVA; LUCIANA BOAVISTA BARROS HEIL; VINICIUS CARNEIRO MORAIS
CAVALCANTI; RAQUEL SOUZA SANTOS; CYNTHIA SANTOS SAMARY; MARIANA MORAES PEREIRA DAS
NEVES ARAÚJO; HANANDA ALYNA POGGIO; LIGIA DE ALBUQUERQUE MAIA; CÍNTIA LOURENÇO
SANTOS; FATIMA CARNEIRO FERNANDES; NIVALDO RIBEIRO VILLELA; PEDRO LEME SILVA; PATRICIA
RIEKEN MACEDO ROCCO
Rationale: In the obese population, anesthesia requires caution due to possible interactions with
the inflammatory status observed in obesity. We compared the short-term effects of
dexmedetomidine vs. propofol on lung morphofunction and markers of inflammation and oxidative
stress in a rat model of diet-induced obesity.
Methods: Wistar rats (n=56) were randomly fed a standard diet (controls) or experimental diet
(obese group) for 12 weeks. Obese animals (n=28) then received sodium thiopental
intraperitoneally and were randomly allocated into 4 subgroups: non-ventilated (n=4), for
molecular biology analysis; thiopental (n=8); propofol (n=8); and dexmedetomidine (n=8). All
received continuous IV administration of the corresponding agents and were mechanically
ventilated for 1 h.
Results: Compared to controls, obese rats had greater visceral fat weight (p=0.02), airway
resistance (p<0.01), and IL-6 levels (p=0.01) in lung and adipose tissues. In obese rats, propofol,
but not dexmedetomidine, increased airway resistance (p<0.01), bronchoconstriction (p=0.02),
and TNF-α and IL-6 levels, and reduced nuclear factor-erythroid 2-related factor-2 and glutathione
peroxidase (p=0.001).
Conclusions: In this obesity model, propofol impaired lung mechanics and histology, increased
lung inflammation, and blunted antioxidant enzymes. However, short-term infusion of
dexmedetomidine had no impact on lung morphofunctional and biological parameters.
08.026 - EFFECTS OF DASATINIB ON LUNG INFLAMMATION AND REMODELING IN EXPERIMENTAL
ALLERGIC ASTHMA
ADRIANA LOPES DA SILVA1; RAQUEL FERREIRA MAGALHÃES 1; VIVIAN ALVES CASTELO BRANCO DA
SILVA1; JOHNATHAS DUTRA SILVA1; FERNANDA FERREIRA CRUZ1; PATRICIA SILVA MARQUES1;
TATIANA PAULA TEIXEIRA FERREIRA2; MARCELO MARCOS MORALES 1; MARCO AURELIO MARTINS2;
PRISCILLA CHRISTINA OLSEN1; PATRICIA RIEKEN MACEDO ROCCO1
1.UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL; 2.FIOCRUZ, RIO DE
JANEIRO, RJ, BRASIL.
Rationale: Asthma is characterized by chronic lung inflammation and airway hyperresponsiveness.
Despite recent advances in understanding its pathophysiology, asthma remains a major public
health problem, highlighting the need for investigation of new therapeutic strategies. In this
context, treatment with dasatinib may reduce inflammatory and remodeling processes, thus
improving lung function, in a murine model of asthma.
Methods: BALB/c mice were randomly assigned to be sensitized and challenged with sterile
ovalbumin (OVA, n=28) or saline (n=7). 24h after the last challenge, animals were treated with
dasatinib, dexamethasone or saline for 7 consecutive days, every 12 hours. 24 hours after the last
treatment, the animals were euthanized and data collected.
Results: In OVA mice, dasatinib as well as dexamethasone led to a significant reduction in airway
hyperresponsiveness. Dasatinib also attenuated alveolar collapse, contraction index and collagen
fiber deposition and increased elastic fiber content in OVA mice. Concerning the inflammatory
process, dasatinib reduced inflammatory cell influx to the airway and in lung-draining mediastinal
lymph nodes, without inducing the thymic atrophy promoted by dexamethasone.
Conclusions: In this of allergic asthma, dasatinib therapy had beneficial modulatory effects on the
inflammatory process, thus improving lung function, without exerting immunosuppressive effects.
08.027 - COMPARISONS BETWEEN PHOSPHODIESTERASE-5 AND FIBROBLAST GROWTH FACTOR
RECEPTOR-1 INHIBITORS IN MONOCROTALINE-INDUCED PULMONARY ARTERIAL HYPERTENSION
DANIELY DAS GRAÇAS ROBERTO; NATHANE SANTANA FELIX; LUCAS DE MENDONCA; JAQUELINE
SOARES DA SILVA; CYNTHIA SANTOS SAMARY; FERNANDA FERREIRA CRUZ; NAZARETH DE NOVAES
ROCHA; GISELE ZAPATA SUDO; MARIANA ALVES ANTUNES; PATRICIA RIEKEN MACEDO ROCCO;
PEDRO LEME SILVA
OBJECTIVE: To compare the effects of fibroblast growth factor receptor (FGFR)-1 inhibitor (iFGF)
versus a phosphodiesterase-5 inhibitor (sildenafil) on hemodynamics, collagen content, and
growth factors in monocrotaline-induced pulmonary arterial hypertension (PAH).
METHODS: 36 Wistar rats were divided into two groups. In the PAH group, rats received
monocrotaline 60 mg/kg by intraperitoneal (i.p.) injection. In the control (C) group, rats received
saline solution i.p. On day 14, C and PAH animals were randomized to receive oral saline (SAL),
sildenafil (SIL) (20 mg/kg/day), or iFGF (5mg/kg/day) daily for 14 days. On day 28, right
ventricular systolic pressure (RVSP), collagen content, and RT-PCR of fibroblast cell culture were
analyzed.
RESULTS: RVSP was higher in PAH-SAL than C groups. After sildenafil, but not iFGF treatment,
RVSP decreased in comparison to PAH-SAL. Collagen content was lower in PAH-iFGF in comparison
to PAH-SAL and PAH-SIL. Similar behavior was observed in fibroblast cell culture regarding mRNA
expression of procollagens I and III and TGF-beta. The TGF-beta protein level was also reduced in
PAH-iFGF.
CONCLUSION: In this model of PAH, sildenafil treatment improved hemodynamic variables but did
not act on the remodeling process. Conversely, FGFR-1 inhibitor acted on lung remodeling at both
molecular and protein levels.
08.028 - GLUTAMINE TREATMENT IN EXPERIMENTAL PULMONARY AND EXTRAPULMONARY
ACUTE RESPIRATORY DISTRESS SYNDROME
MARIANA ALVES BELLEZZIA1; JAMIL ZOLA KITOKO2; SORAIA CARVALHO ABREU1; MARCELO MARCOS
MORALES2; PRISCILLA CHRISTINA OLSEN 3; PATRICIA RIEKEN MACEDO ROCCO1; GISELE PENA DE
OLIVEIRA1
1.LABORATORY OF PULMONARY INVESTIGATION, RIO DE JANEIRO, RJ, BRASIL; 2.LABORATORY OF
CELLULAR AND MOLECULAR PHYSIOLOGY, IBCCF, FEDERAL UNIVERSITY OF RIO DE JANEIRO, RIO DE
JANEIRO, RJ, BRASIL; 3.LABORATORY OF CLINICAL BACTERIOLOGY AND IMMUNOLOGY, FEDERAL
UNIVERSITY OF RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL.
Rationale: Several experimental studies have demonstrated the beneficial effects and mechanisms
of action of glutamine (Gln) in acute respiratory distress syndrome (ARDS) of extrapulmonary (exp)
etiology; however, no study has evaluated the impact of intravenous (i.v.) Gln administration on
pulmonary (p) ARDS. Since the pathophysiology of ARDS may differ according to the type of
primary insult, leading to activation of different inflammatory mechanisms, we tested the
hypothesis that glutamine might act differently in experimental ARDSp or ARDSexp with similar
mechanical compromise.
Methods: BALB/c animals were randomly divided into two main groups. ARDS animals received E.
coli lipopolysaccharide intratracheally [ARDSp, 10 µg] or intraperitoneally [ARDSexp, 125 µg],
while control (C) animals were not subjected to surgical procedures, injections or instillations.
Six hours after LPS administration, C and ARDS mice were euthanized for evaluation of cell counts
in bronchoalveolar lavage fluid, while another group of animals subjected to the same protocol
were subdivided to receive a single i.v. dose of saline (0.17 mL) or Gln (0.75 g/kg body weight).
At 6 h, lung inflammation was observed in both groups.
Results: 24 h after LPS, i.v. Gln treatment yielded a reduction in static lung elastance (p<0.05),
neutrophil infiltration and alveolar collapse, regardless of ARDS etiology.
Conclusion: Glutamine effectively improved morphofunction regardless of the etiology of ARDS.
08.029 - GHRELIN YIELDS BENEFICIAL EFFECTS IN A MURINE MODEL OF ELASTASE-INDUCED
EMPHYSEMA
CASSIA LISBOA BRAGA1; MILENA VASCONCELLOS DE OLIVEIRA1; LIGIA DE ALBUQUERQUE MAIA1;
GISELE PADILHA1; JOHNATHAS DUTRA SILVA1; PEDRO LEME SILVA1; VERA LÚCIA CAPELOZZI2;
PATRICIA RIEKEN MACEDO ROCCO1; NAZARETH DE NOVAES ROCHA1
1.UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL; 2.UNIVERSIDADE DE
RATIONALE: Ghrelin, a natural endogenous ligand of the growth hormone secretagogue receptor,
has anti-inflammatory properties and may have a cardiovascular protective effect. We
hypothesized that ghrelin would have beneficial pulmonary and cardiovascular effects in
experimental emphysema.
METHODS: Emphysema (E) was induced in C57/BL6 mice (n=25) by intratracheal administration of
porcine elastase once weekly for 4 weeks. A control group (C, n=20) received saline. After 2
weeks, C and E animals received ghrelin (200 µg/kg/day intraperitoneally) or saline for 3 weeks.
Dual-energy X-ray absorptiometry and echocardiography were performed before and after saline
or ghrelin administration for analysis of body composition and cardiovascular function. One week
after the last dose, lung morphofunction was analyzed.
RESULTS: E animals had a larger right ventricular area (RVA) and lower pulmonary
acceleration/pulmonary ejection time ratio (PAT/PET) compared to C. In the E group, ghrelin
significantly increased fat and lean mass, reduced RVA and increased PAT/PET, and reduced
increases in KC (mouse analog of interleukin-8). On ultrastructural analysis, ghrelin yielded
alveolar repair and increased the number of type 2 epithelial cells. Although static lung elastance
was lower in E compared to C animals (p<0.01), ghrelin yielded no mechanical changes.
CONCLUSION: In the current model of emphysema, ghrelin improved body composition and
cardiovascular function with no significant changes in lung function.
08.030 - MAS RECEPTOR IS CRITICAL FOR THE BENEFICIAL EFFECTS INDUCED BY EXERCISE
TRAINING IN A MODEL OF ASTHMA
JULIANA FABIANA GREGORIO; GISSELLE SANTOS MAGALHÃES; PUEBLA CASSINI VIEIRA; MARIA DA
GLÓRIA RODRIGUES MACHADO; MARIA A RIBEIRO VIEIRA; ROBSON AUGUSTO SOUZA DOS SANTOS;
MARIA JOSE CAMPAGNOLE-SANTOS
Angiotensin-(1-7) [Ang-(1-7)] treatment or exercise training (ET) attenuates pulmonary
inflammation and remodeling in chronic models of asthma. In this study we evaluated the
involvement of the Mas receptor in the beneficial effects induced by ET in asthma. Mice were
subjected to ovalbumin (OVA) sensitization (20 μg/mouse, ip, 4 doses 14 days apart) and challenge
(OVA 1% nebulization/30 min, 3 times/week, for 4 weeks) to induce chronic allergic lung
inflammation (OVA mice). Five days after the 2nd OVA sensitization, mice started to receive
infusion (osmotic minipump) of the Mas receptor antagonist, A779 (1 µg/h) or saline (5 µl/h) for
28 days and 2 days later, mice started ET (moderate intensity on a treadmill, 1h/day, 5
times/week, 4 weeks). Histologic analysis was performed in the left lung and evaluation of
eosinophils and neutrophils infiltration was measured by determination of eosinophil peroxidase
(EPO) and myeloperoxidase (MPO) activity in the lung. OVA mice showed worse performance in a
maximal exercise test in comparison with ET and OVA+ET mice. OVA mice, in relation to control,
presented increased collagen deposition (34±2.0 vs 15±1.9 au), EPO (0.38±0.03 vs 0.25±0.04
OD/mg) and MPO (1.55±0.14 vs 0.95±0.13 OD/mg) activity, which were reduced by ET (17±2.1 au,
0.24±0.02 OD/mg, 1.02±0.06 OD/mg, respectively). Treatment of OVA+ET mice with A779
prevented these effects, suggesting that Ang-(1-7)/Mas axis is important for the attenuating
effects induced by ET in asthma.
THEME 09 - ENDOCRINE & METABOLISM
28
Furthermore, lipid inclusion was observed in the liver from CAF animals (p<0.05). Conclusions:
Maternal CAF consumption during lactation program to obesity late in life associated to hepatic
insulin resistance and increase of gluconeogenesis. Milk composition unbalanced could be a target
mechanism for this programming.
09.023 - EVALUATION OF QUALITY AND DURATION OF SLEEP AND CORTISOL SALIVARY LEVELS
IN CHILDREN WITH ASTHMA AND OVERWEIGHT/OBESITY
NAYARA DOS SANTOS VIEIRA; TAÍS DE SOUZA BARBOSA
FACULDADE DE ODONTOLOGIA DE PIRACICABA/UNICAMP, LIMEIRA, SP, BRASIL.
This study aimed to evaluate the quality and duration of sleep and salivary cortisol levels in 55
children, 15 with asthma (GA), 12 with overweight/obesity (GSO), with asthma and
overweight/obesity (GASO) and 15 controls (CG), of both genders, between seven and nine years
old. Weight, height, body mass index (BMI), triceps skinfold (TSF) and subscapular (DCS) and the
arm circumferences (AC) and abdominal (CA) were measured. It was used the brazilian version of
"The Sleep Disturbance Scale for Children" (SDSC) to assess the behavioral patterns of the child
related to sleep. Saliva samples were collected in two days of the week (one day on the weekend)
and in three periods: in the morning, just after waking and 30 minutes after waking; and at night
before bed. The male gender was more prevalent in GA (60%) and GASO (69.2%), while the females
in GSO and GC. The GSO and GASO showed greater anthropometric values. The GSO showed higher
score EDSC, in spite of not significant, suggesting greater impairment in quality and duration of
sleep. Although not significant, the greatest response upon waking was observed in the GSA, in
the weekday, and GASO, over the weekend. The latter group had less daytime decline of cortisol
in both the weekday and on the weekend, reflecting the lower amplitude in the circadian rhythm
of cortisol.
09.024 - DEXAMETHASONE DECREASES HYPOTHALAMIC AND NEUROHYPOPHYSEAL HORMONAL
RELEASE INDUCED BY HYPEROSMOLALITY IN VITRO
FERNANDA MARIA VEANHOLI VECHIATO1; RICARDO COLETTI1; JULIANA BEZERRA MEDEIROS DE
LIMA1; SILVIA GRACIELA RUGINSK2; LUCILA LEICO KAGOHARA ELIAS1; JOSÉ ANTUNES-RODRIGUES1
1.FMRP/USP, RIBEIRAO PRETO, SP, BRASIL; 2.UNIFAL-MG, ALFENAS, MG, BRASIL.
The glucocorticoids seem to modulate magnocellular neurons which promote hypothalamic
synthesis of vasopressin (AVP), oxytocin (OT) and atrial natriuretic peptide (ANP) besides AVP and
OT release by neurohypophysis (NH). However, the effect of glucocorticoids on hyperosmolalityinduced hormone release in vitro has not yet been well elucidated. Therefore, we evaluated the
glucocorticoids effects, through dexamethasone (Dexa) incubation, on hormonal secretion in
response to hyperosmolality in vitro. Adult male Wistar rats’ medial basal hypothalamus (MBH)
and NH were collected and pre-incubated in isotonic (280 mOsm/Kg H2O) oxygenated Krebs–Ringer
bicarbonate buffer (KRBG), at 37°C, 50 cycle/min, for 60 min. The medium was replaced with
KRBG medium (280 or 340 mOsm/Kg H2O, the latter made by the addition of mannitol or NaCl)
for 30 min, containing or not Dexa (1, 10 e 100 nM). MBH and NH exposure to hypertonic KRBG,
regardless of whether hyperosmolality was induced by mannitol or NaCl, increased AVP, OT and
ANP. Dexa promoted a dose-dependent decrease on AVP, OT and ANP release by the MBH and NH
under mannitol-induced hyperosmolality. At the 1 nM concentration, Dexa also decreased
hormone release by the MBH and NH induced by hyperosmotic stimulus performed with NaCl. Our
data showed for the first time that Dexa reduced osmolality-induced AVP, OT and ANP release by
MBH and AVP and OT by NH, regardless of sodium extracellular concentrations.
09.025 - DIABETIC RATS FED WITH DIETS SUPPLEMENTED WITH STEVIA REBAUDIANA LEAVES
HAVE BETTER METABOLIC CONTROL
GISLENE BONGIOVANI; YAGO CARVALHO LIMA; PAULA GIMENEZ MILANI; SÍLVIO CLÁUDIO DA COSTA;
CECILIA EDNA MAREZE DA COSTA
The Stevia rebaudiana, native plant from the border between Brazil and Paraguay, is a source of
natural non-caloric sweeteners. Antidiabetic properties of the total extract or isolated products
of stevia were found in recent decades. However, this is the first study to report the chronic
effects of ration supplementation with stevia leaves on the metabolic control of diabetic rats.
Dried and milled leaves of Stevia rebaudiana were added to the Nuvilab® ration in a proportion
of 2%. As control, 2% of carboxymethylcellulose was added to the ration. Eexperimental diabetes
was induced in 60-day-old male Wistar rats by intraperitonial injection of streptozotocin (45
mg/kg). Animals with fasting blood glucose levels greater than or equal to 200mg/dl were
selected. Diabetic animals fed ration supplemented with stevia leaves (four week treatment;
n=11-16 rats) presented lower plasma levels of glucose (473,4±41,9 vs 354,4±30,6mg/dL),
triglycerides (149,1±20,3 vs 83,0±7,5 mg/dL) and total cholesterol (69,6±4,1 vs 54,9±2,7 mg/dL).
The amount of liver glycogen was higher in the diabetic animals fed ration supplemented with
stevia leaves compared with the control group. Conclusions: The ration supplemented with 2% of
Stevia rebaudiana leaves improved the glycemic and lipid control in diabetic rats, without
affecting food intake and body weight gain.
09.021 - MATERNAL CAFETERIA-STYLE DIET DURING LACTATION INDUCES OBESITY AND ALTERS
HYPOTHALAMIC NPY CONTENT IN ADULT OFFSPRING
FLÁVIO ANDRADE FRANCISCO; RODRIGO MELLO GOMES; LUCAS PAULO JACINTO SAAVEDRA; CARINA
PREVIATE; ISABELA PEIXOTO MARTINS; VERIDIANA MOTA MOREIRA; LAIZE PERON TÓFOLO; ROSIANE
APARECIDA MIRANDA; VILMA A.F. GODOI; PAULO CESAR DE FREITAS MATHIAS; CLAUDINÉIA
CONATIONI DA SILVA FRANCO; MARIA RAQUEL MARÇAL NATALI
Background: During pregnancy and lactation pups are vulnerable to nutritional or hormonal
changes of the mother, which increase risk to develop metabolic diseases later in life (DOHaD
concept). We aim was to understand if maternal diet and breast milk composition can program
offspring to develop metabolic changes in adulthood associated to hypothalamic leptin and insulin
crosstalk and neuropeptide Y (NPY) expression. Methods: Female Wistar rats were divided into
two groups: control (CO) fed with standard chow, and cafeteria group (CAF) fed with cafeteriastyle diet. All pups received CO diet from weaning until 90 days-old. Results: The breast milk from
CAF mothers contains more cholesterol, triglycerides and protein (p<0.05). CAF offspring had
overweight and hyperphagia, which was associated with an increase in the adiposity of this animals
(p<0.05). Leptin and insulin hypothalamic crosstalk decreased by expression of Irβ, ObRb, and
JAK2, suggesting hypothalamic resistance to this hormones, associated with an increase
hypothalamic NPY content. Furthermore, CAF pups presented insulin resistance. Conclusions:
Maternal cafeteria-style diet during lactation changes breast milk composition that program
offspring to develop obesity at adulthood that is associated with disruption hypothalamic leptin
and insulin crosstalk and increased NPY expression.
09.026 - REPROGRAMMING OBESITY: MATERNAL LOW INTENSITY EXERCISE DURING PERINATAL
LIFE PROTECTS OFFSPRING FROM METABOLIC CONSEQUENCES OF EARLY OVERNUTRITION
AUDREI PAVANELLO; TATIANE APARECIDA RIBEIRO; ANANDA MALTA; LAIZE PERON TÓFOLO; ISABELA
PEIXOTO MARTINS; RODRIGO MELLO GOMES; PAULO CESAR DE FREITAS MATHIAS
Background: Changes in the environment during developmental periods of life can lead to
profound metabolic changes in the offspring. Recent studies showed that stressful conditions can
program the metabolism to develop metabolic diseases latter in life. Furthermore, physical
exercise can ameliorate metabolic consequences in overweight and obese people. In this
context, we question if the metabolism off the offspring can be programmed to be resistant to
deleterious metabolic consequences latter in life. Methods: Rat dams were submitted to run in a
treadmill during all pregnancy and lactation, 3-times/week with speed set to 30% of VO2max.
Sedentary dams did not train. After birth, litters were adjusted to 9 pups (normal-litter, NL) and
3 (small-litter, SL) to induce obesity. Body weight (bw) and food intake were registered from
mothers and offspring. Ninety-days-old adult offspring were used to evaluate the metabolism.
Results: Exercise increased insulinemia in mothers; but those changes did not affect bw and food
intake. Litter size reduction induced obesity, hyperinsulinemia, glucose intolerance and insulin
resistance (p<0.05). Maternal exercise training ameliorated the metabolic deleterious
consequences of SL rats, with normalization of body weight, fat pad deposition, glucose
intolerance, insulinemia, and glycemia (p<0.05). Conclusion: Maternal low intensity exercise
blocks obesity onset induced by early overnutrition latter in life.
09.022 - HEPATIC INSULIN RESISTANCE AND INCREASED GLUCONEOGENESIS IS CAUSED BY
MATERNAL CAFETERIA-STYLE DIET INTAKE DURING LACTATION
FLÁVIO ANDRADE FRANCISCO; RODRIGO MELLO GOMES; LUCAS PAULO JACINTO SAAVEDRA; CARINA
PREVIATE; ISABELA PEIXOTO MARTINS; VERIDIANA MOTA MOREIRA; LAIZE PERON TÓFOLO; ROSIANE
APARECIDA MIRANDA; VILMA A.F. GODOI; PAULO CESAR DE FREITAS MATHIAS; CLAUDINÉIA
CONATIONI DA SILVA FRANCO; MARIA RAQUEL MARÇAL NATALI
Background: Enhanced gluconeogenesis, hepatic insulin resistance and non-alcoholic fatty liver
diseases (NAFLD) are associated with obesity onset. During perinatal life, pups are vulnerable to
stressful factors, which increase risk to develop metabolic diseases later in life. Thus, unbalanced
milk composition can program offspring to metabolic syndrome. Using cafeteria-style diet (CAF)
during lactation, we aimed test whether offspring displays obesity associated to hepatic
malfunction at adulthood. Methods: Lactating Wistar rats were divided in two groups CAF: fed CAF
during lactation and CO: fed standard chow diet. All pups received standard chow from weaning
until 90 days-old. Results: Milk from CAF mothers showed increased cholesterol, triglycerides and
protein (p<0.05). Adult offspring from CAF group presented: overweight, enhanced adiposity and
dyslipidemia (p<0.05). Hepatic insulin resistance was recorded, with lower expression of insulin
transduction signal proteins PI3K and Akt (p<0.05). Increased hepatic gluconeogenesis was
demonstrate in pyruvate tolerance test and high expression of PEPCK and G6Pase (p<0.05).
09.027 - PRIVAÇÃO DE SONO REDUZ FATORES DE CRESCIMENTO E PREJUDICA A REGENERAÇÃO
MUSCULAR
MARCOS MÔNICO-NETO; MURILO DÁTTILO; HELTON DE SÁ SOUZA; SARA QUAGLIA DE CAMPOS
GIAMPÁ; KIL SUN LEE; SERGIO TUFIK; HANNA KAREN MOREIRA ANTUNES
UNIFESP, SAO PAULO, SP, BRASIL.
Privação de sono (PS) aumenta as concentrações de hormônios catabólicos e reduz fatores de
crescimento envolvidos na regeneração muscular, o que pode dificultar a recuperação de lesões
musculares. O objetivo foi averiguar a influência da privação de sono na regeneração muscular e
a concentração muscular de IGF-1 após criolesão em ratos. Trinta ratos machos, Wistar, 3 meses,
foram distribuídos em 3 grupos: controle (CTL), grupo PS por 96h (PS96) e grupo PS por 96h mais
recuperação de sono por 96h (PS96+R). Previamente os animais foram submetidos cirurgicamente
à criolesão no músculo tibial anterior (TA) da pata traseira direita, após, os grupos PS96 e PS96+R
foram privados de sono por 96h. Ao término da PS, o grupo PS96+R permaneceu na caixa moradia
por mais 96h, com sono ad libitum. O TA das patas traseiras (sadia e lesionada) foi dissecado e
realizado análises histopatológicas qualitativas e o IGF-1 muscular foi mensurado. A concentração
de IGF-1 muscular da pata sadia foi reduzida no grupo PS96 e os valores basais foram
restabelecidos no grupo PS96+R. Na pata lesionada, as concentrações de IGF-1 aumentaram em
todos os grupos, porém com magnitude menor nos grupos PS96 e PS96+R em relação ao CTL. Na
análise histológica, foi observado um atraso no processo de regeneração da pata lesionada, com
maior área de tecido fibroso no grupo PS96+R comparado ao CTL. PS reduziu a concentração
muscular de IGF-1 e atrasou a regeneração estimulada por criolesão no músculo TA de ratos.
09.028 - EVALUATION OF THE (ANTI)ANDROGENIC EFFECT OF TRICLOSAN IN THE HERSHBERGER
TEST
BRUNO GARCIA MONTAGNINI; NATHALIA ORLANDINI COSTA; MARINA RANGEL FERRO PEREIRA;
KARINE VANDRESSA PERNONCINI; BRUNA CAROLINE PIERONE; DANIELA CRISTINA CECCATTO
GERARDIN
UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL.
Triclosan (TCS) is an antibacterial compound widely used in cosmetics and personal care products.
This study was designated to evaluate if TCS has (anti)androgenic effect in the Hershberger test
(OCSPP 890.1400, EPA). On postnatal day (PND) 52, castrated male rats (n=6/group) were orally
treated for 10 consecutive days with TCS at different doses (0.8; 2.4; 8.0 and 24.0 mg/kg) or
vehicle control (corn oil, 2.5 ml/kg). Testosterone (T) (0.4 mg/kg, ip) was administered as a
positive control for androgenicity. For anti-androgenic evaluation, Flutamide (FLUT) (3 mg/kg)
plus T were administered as a positive control group and additional groups were treated with TCS
at 4 different doses plus T. On PND 63, ventral prostate, seminal vesicle, levator anibulbocarvenosus muscle, paired Cowper’s glands and glans penis were removed and weighed
(CEUA/UEL: 283.2015.27). Data±SEM were compared by ANOVA (*p<0.05). No statistical
differences were found in final body weight among groups. Androgenic and anti-androgenic effects
were observed only in T group and in FLUT plus T, respectively, for example: prostate weight (g)
(vehicle: 0.007±0.001/ T: 0.096±0.001*/ T+FLUT: 0.041±0.006#/ TSC 0.8+T: 0.110±0.010/ TCS
2.4+T: 0.099±0.013/ TCS 8.0+T: 0.090±0.014/ TCS 24.0+T: 0.102±0.008/ TCS 0.8: 0.005±0.001/
TCS 2.4: 0.006±0.002/ TCS 8.0: 0.008±0.002/ TCS 24.0: 0.008±0.002) (*p<0.05 compared to
vehicle; #p<0.05 compared to T; ANOVA, complemented with Dunnett’s test). The results indicate
that the TCS is unable to elicit (anti)androgenic activities at tested doses.
09.029 - IDENTIFICATION OF THE MOLECULAR MECHANISMS INVOLVED IN THE PERIPHERAL
INSULIN RESISTANCE IN CACHETIC SOLID EHRLICH CARCINOMA-BEARING MICE
LEVY MALMONGE MARTIN NETO1; NATALIA MORETI VIOLATO1; PRISCILLA PONCE VAREDA1;
NATHALIA APARECIDA DE PAULA CAMAFORTE1; KARINA NOMIDOME SENNA2; HENRIQUE JORGE
NOVAES MORGAN2; JOSÉ ROBERTO BOSQUEIRO3
1.IBB - UNESP, BOTUCATU, SP, BRASIL; 2.DCP - UNESP, BAURU, SP, BRASIL; 3.DEF - UNESP, BAURU,
SP, BRASIL.
Insulin produced by the beta cells of the pancreatic islets bind itself with its surface receptor in
the plasmatic membrane and activates a phosphorylation cascade, resulting in glucose uptake on
the peripherical tissues. Any defect in these events results in a metabolic condition called insulin
resistance often observed in patients with large tumors and also associated with cachexia,
characterized by loss of adipose tissue and muscle wasting and weight loss. In previous studies,
we found that after 14 days of inoculation of Solid Ehrlich Tumor (SET) in mice, the animals,
unexpectedly, showed higher insulin sensitivity and at 21 days, the animals were resistant to
insulin, as expected for cachetic patient, described in the literature. Given these facts, we use
Western blotting Assay to evaluate the protein expression of the insulin signaling pathway in liver
and muscle of cachectic mice bearing solid Ehrlich tumor at 14 and 21 days of inoculation. AKTPKB
protein, was shown to be reduced in the liver, characteristic from a resistance tissue, while in
muscle, the IRS1 protein phosphorylated in serine, its inactive form, the expression rises in TSE14
group compared to the CTL. The results obtained so far show us a deficiency in signaling pathway,
but additional studies are needed to better understand the metabolic condition.
09.030 - O CONSUMO MATERNO DE DIETA HIPERLIPÍDICA PROMOVE ALTERAÇÔES SEXOESPECÍFICAS NO CORAÇÃO DA PROLE JOVEM
SUELEN GUEDES ZECA1; TAINA DE OLIVEIRA MORAIS1; NATÁLIA D' ASSUMPÇÃO LIMA RANGEL1;
DEBORA BARBOSA AMARANTE1; ISALIRA PEROBA RAMOS2; GEORGE EDUARDO GABRIEL KLUCK2;
LUCIANO GONÇALVES FERNANDES1; CARMEN CABANELAS PAZOS-MOURA2; ISIS HARA TREVENZOLI2;
NORMA APARECIDA DOS SANTOS ALMEIDA1
1.UFRRJ, SEROPÉDICA, RJ, BRASIL; 2.UFRJ, RIO DE JANEIRO, RJ, BRASIL.
O consumo materno de dieta hiperlipídica (DH) promove obesidade, hiperleptinemia e aumento
sérico de T3 em filhotes machos, ao desmame. O objetivo deste trabalho foi investigar se o
consumo materno de DH é capaz de alterar a expressão ventricular dos receptores β1-AR e TRα1,
o acúmulo de lipídeos neutros e a função cardíaca da prole jovem de ambos os sexos. Ratas wistar
consumiram dieta controle (C), 9% lipídeos ou DH, 29%, por 7 semanas, gestação e lactação. Ao
desmame, parte das proles foi eutanasiada e os ventrículos foram coletados. O restante das proles
teve acesso ad libitum à dieta C até 30 dias, quando análises funcionais foram feitas. A prole DH
(PDH) apresentou maior peso ventricular (+24%). Houve aumento da expressão do β1-AR nos
filhotes fêmeas (PDHF) (+93%) e do TRα1 nos machos (PDHM) (+2x). O conteúdo de colesterol
esterificado apresentou-se menor nos PDHM (-48%), enquanto o conteúdo de TAGs apresentou
aumento nos PDHM (+1x) e PDHF (+94%). Aos 30 dias, a PDH apresentou redução na fração de
ejeção do VE (-5%). Foi observado prejuízo sistólico (+33% volume sistólico final) nos PDHF, ao
passo que hipertrofia (+28% massa do VE) foi observada apenas nos PDHM. Nenhuma alteração foi
observada no eletrocardiograma ou balanço autonômico cardíaco. Os resultados demonstram que
o consumo materno de DH promove alterações precoces na função cardíaca independente do sexo
do filhote, no entanto machos e fêmeas apresentam mecanismos distintos no desenvolvimento da
disfunção.
09.031 - EXERCISE TRAINING MODULATES THE GLUCONEOGENESIS AND INFLAMMATION IN
FRUCTOSE-FED RATS
FERNANDA MARQUES FREITAS; MARIANA SODRÉ BOÊTA ANDRADE; ISABELE GOMES GIORE; ELIETE
DALLA CORTE FRANTZ; RENATA FRAUCHES MEDEIROS; THAIS BENTO-BERNARDES; THAIANE GADIOLI
GAIQUE; CAROLINE FERNANDES SANTOS; KAREN JESUS OLIVEIRA; ANTONIO CLAUDIO LUCAS DA
NÓBREGA
Introduction: Metabolic syndrome (MS) is characterized by a cluster of related morbidities which
includes nonalcoholic fatty liver disease (NAFLD). We investigated the effects of exercise training
in the MS model.
Methods: Wistar rats were divided into control and high fructose (fructose in drinking water) group
for 2 weeks. Then the animals were subdivided into (n=8) control (CT), exercise control (CT-Ex),
high fructose (HFr) and exercise high fructose (HFr-Ex) groups for an exercise protocol (additional
8 weeks). The exercise was assigned to treadmill training at moderate-intensity (60 min/day, 4
days/week). We assessed body mass, maximal exercise test, glucose metabolism, protein
expression of hepatic gluconeogenesis and inflammation.
Results: Neither fructose overload nor exercise training influenced body mass gain. The training
groups increased post-training maximal speed compared to untrained groups. The HFr group
showed hyperinsulinemia and reduced insulin sensitivity, but exercise training normalized these
parameters. Exercise training was effective in preventing hepatic glycogen accumulation.
Furthermore, exercise improved the response to the deleterious effects of HFr overload by
normalizing the PEPCK and GLUT2 expression as well as the protein levels of IL-6 and TNF-alpha.
Conclusion: Exercise training could be responsible for the suppression of inflammatory pathways
and recoveries of glucose homeostasis through improve of gluconeogenesis associated with
fructose ingestion.
09.032 - AUMENTO DE CÉLULAS MICROGLIAIS NO NÚCLEO ARQUEADO EM MODELO DE
PROGRAMAÇÃO NUTRICIONAL NEONATAL
LUCAS KNIESS DEBARBA; BEATRIZ C. BORGES; PAULA BEATRIZ MARANGON; JADE CABESTRE
VENANCIO; GISLAINE ALMEIDA-PEREIRA; PRISCILA MARLYS SÁ RIVAS; JOSÉ ANTUNES-RODRIGUES;
LUCILA LEICO KAGOHARA ELIAS
FACULDADE DE MEDICINA DE RIBEIRÃO PRETO - USP, RIBEIRAO PRETO, SP, BRASIL.
As alterações nutricionais no período neonatal são capazes promover aumento da atividade
inflamatória no indivíduo com consequente alteração metabólica. Objetivou-se avaliar os efeitos
da alteração nutricional durante o período neonatal sobre o ganho de peso corporal e a contagem
de células microgliais no hipotálamo de ratos wistar. Após o nascimento, foram mantidos 3
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filhotes, ninhada pequena – SL, 10 filhotes, ninhada normal – NL ou 16 filhotes, ninhada grande –
LL de machos com cada fêmea. Foram mantidos em ambiente com temperatura e ciclo claroescuro controlados, dieta padrão e água ad libitum. O ganho de peso corporal da ninhada foi
mensurado até o 60º dia de vida. Os animais foram perfundidos no 21º e 60º dia, n=6 cada e foi
realizada imuno-histoquímica do tecido cerebral. O grupo SL apresentou aumento no ganho do
peso antes (F10,50=10,407; p<0,0001) e após (F18, 54=5,5892; p<0,0001) o desmame quando
comparado ao grupo NL. O oposto foi observado no grupo LL quando comparado ao grupo NL
(p<0,05). O grupo SL no 21º dia (F2, 18=22,764, p=,00001) e no 60º dia (F2, 12=4,6509; p=,03196)
apresentou um aumento (p<0,01) na quantidade de células microgliais no arqueado quando
comparado ao grupo NL. Adicionalmente, no 60º dia o grupo LL apresentou um aumento nas células
microglias (p<0,05) quando comparado ao grupo NL. Conclui-se que a programação nutricional
neonatal é capaz de promover alterações na homeostase energética relacionada com o aumento
das células microgliais.
09.033 - LOW-PROTEIN DIET DURING LACTATION LEADS TO A LEAN PHENOTYPE AND INSULIN
SENSITIVITY IN RATS FED WITH A HIGH-FAT DIET IN ADULTHOOD
ISABELA PEIXOTO MARTINS1; ANANDA MALTA1; CARINA PREVIATE1; AUDREI PAVANELLO1; TATIANE
APARECIDA RIBEIRO1; ROSIANE APARECIDA MIRANDA1; PAULO CESAR DE FREITAS MATHIAS 1; JÚLIO
CEZAR DE OLIVEIRA2
1.UNIVERSIDADE ESTADUAL DE MARINGÁ, MARINGA, PR, BRASIL; 2.UNIVERSIDADE FEDERAL DO
MATO GROSSO, SINOP, MT, BRASIL.
Introduction/Aims: Obesity and metabolic syndrome has been correlated to insults in perinatal
life. We studied the effect of a high-fat diet in animals that were malprogrammed by low-protein
diet during the suckling phase.
Methods: Female Wistar rats received a low-protein diet (4%) during the first two weeks of
lactation, while controls dams were fed with a normal-protein diet (23%). At weaning, male rat
offspring received a control diet until 60 days of life, when half of both groups were fed a highfat diet (35%, NP/HF and LP/HF groups) or a control diet (7%, NP/NF and LP/NF groups). Final
body weight was evaluated at 90-day-old, when the animals were submitted to ivGTT and ipITT
tests. Then, they were euthanized and mesenteric fat pad store was weighed.
Results: LP/NF were leaner when compared with NP/NF rats (p<0.01). High-fat diet increased
body weight in both groups, but the magnitude was bigger in NF/HF (120,9%) than LP/HF (115,6%).
Mesenteric fat pad were increased in both groups that received a high-fat diet (+143,07% NP/HF
and +102,22% LP/HF). Only NP/HF group was glucose intolerance (+128,34%) and insulin resistant
by Kitt (%)/min (-52,52%).
Conclusions: Adult rat malprogrammed by low-protein diet during lactation are resistant to
metabolic dysfunctions induced by a high-fat diet in adulthood.
09.034 - EFEITO DE DIETA INDUTORA DE OBESIDADE NA EXPRESSÃO DE KISSPEPTINA E FUNÇÃO
GONADAL EM RATO
LAISA TALINE MAGALHAES HIPOLITO; JUNEO FREITAS SILVA; CÂNDIDO CELSO COIMBRA; RAPHAEL
ESCORSIM SZAWKA
UNIVERSIDADE FEDERAL DE MINAS GERAIS (UFMG), BELO HORIZONTE, MG, BRASIL.
Os mecanismos pelos quais a obesidade causa infertilidade masculina são pouco conhecidos.
Assim, investigamos em ratos se a diminuição da expressão hipotalâmica de kisspeptina pode ser
o caminho central que interliga a obesidade com a supressão do eixo reprodutivo. Ratos machos
recém-desmamados foram tratados com dieta hiperlipídica ou padrão ad libitum durante treze
semanas. Avaliou-se a ingestão calórica, intolerância à glicose, peso corporal, de coxins adiposos,
próstata, vesícula seminal e testículos. Realizou-se histomorfometria dos testículos e
imunohistoquímica para kisspeptina no núcleo arqueado (ARC). Ratos obesos apresentaram maior
peso corporal, ingestão calórica e intolerância à glicose comparado com o grupo controle (P <
0.05). Os coxins adiposos retroperitoneal, mesentérico e gonadal estavam aumentados, enquanto
que os pesos relativos da próstata, vesícula seminal e testículos estavam menores nos ratos obesos
em relação aos controles (P < 0.05). Análise morfológica dos testículos revelou degeneração do
epitélio seminífero multifocal moderada, com maior porcentagem de túbulos degenerados no
grupo obeso em relação ao controle (P < 0.05). Os ratos obesos apresentaram redução de
aproximadamente 30% de neurônios imunorreativos à kisspeptina no ARC em relação aos controles
(P < 0.05). Conclui-se que a dieta hiperlipídica inibe a expressão de kisspeptina no ARC, efeito
que parece ser responsável pela supressão do eixo gonadal e degeneração testicular decorrentes
da obesidade.
09.035 - METABOLIC SHORT TERM EFFECT OF EXPOSITION TO AMBIENT AIR PARTICULATE
MATTER DURING PERINATAL LIFE DEPENDS ON SEXUAL DIMORPHISM
ROSIANE APARECIDA MIRANDA; EVANDRO JOSÉ BERALDI; FLÁVIO ANDRADE FRANCISCO; ISABELA
PEIXOTO MARTINS; CARINA PREVIATE; VANDER SILVA ALVES; CLAUDINÉIA CONATIONI DA SILVA
FRANCO; KESIA GEMIMA PALMA; RODRIGO MELLO GOMES; PAULO CESAR DE FREITAS MATHIAS
STATE UNIVERSITY OF MARINGA, MARINGÁ, PR, BRASIL.
Background: The association of urbanization with ambient air particulate matter (PM) exposure
contributes to respiratory disorders and affects metabolism. Nevertheless, there are no sufficient
studies evaluating the impact of ambient air PM exposition during perinatal life. We investigated
the short-term effects of PM exposition during perinatal life on male and female offspring
metabolism.
Methods: Samples of <10 µm particulate matter (PM<10) were collected in an urban area of
Cotonou, Benin. The PM<10 solution was diluted in corn oil. Throughout pregnancy and lactation,
dams were gavaged with 200 µg of PM<10/kg of body weight/day. After birth, the metabolism of
dams and their 21-day-old male and female offspring were assessed.
Results: Gestational and lactational PM administration did not alter maternal glycemic
homeostasis. However, in the exposed progeny, male offspring were normoglycemic, but
presented hyperinsulinemia, insulin resistance, dyslipidemia and overweight (p < 0.05). Female
offspring did not present any alterations.
Conclusion: Exposition to ambient particulate matter (PM<10) during perinatal life alters the
metabolism of male rats offspring only, demonstrating a different mechanism depending on sexual
dimorphism.
09.036 - CORTISOL E GLUTAMINA DIPEPTÍDEO RECUPERAM A GLICEMIA DURANTE HIPOGLICEMIA
INDUZIDA POR INSULINA (HII) EM CAMUNDONGOS SWISS DIABÉTICOS TIPO 1
CAMILA BATAGLINI; SILVIA CARLA SANTANA AZEVEDO; DIEGO G.L. REZENDE; MARCOS A. PRIMO;
JULIANA NUNES DE LIMA MARTINS; NAYRA DELATORRE BRANQUINHO; VALÉRIA SCHOFFEN ROMÃO
CARRASCOZA; MARIA M.D. PEDROSA; VILMA A.F. GODOI
UEM, MARINGA, PR, BRASIL.
O principal tratamento para indivíduos portadores de Diabetes Mellitus tipo 1 (DM1) é a
insulinoterapia intensiva que, por sua vez, aumenta a incidência de hipoglicemia induzida por
insulina (HII). Por isto, estudamos se os hormônios contrarreguladores associados a GDP (glutamina
dipeptídeo) podem prevenir uma crise de HII. Foram utilizados camundongos Swiss machos
normoglicêmicos e diabéticos tipo 1 (STZ, 60 mg/Kg). Os animais (jejum 15h) receberam, por via
intraperitoneal, insulina (1U/kg) e glucagon (0,02mg/Kg) ou cortisol (20mg/Kg) ou adrenalina
(1mg/Kg), e estes com ou sem GDP oral (400mg ou 1g/Kg). A glicemia foi monitorada nos tempos
0,15,30,60,120,180,240,300 min (5h). A crise hipoglicêmica foi avaliada aos 60min, e a
recuperação da glicemia aos 300min. Dados: média±dp, n=8/grupo, teste t, p<0,05. O grupo
insulina apresentou os valores glicêmicos 0min: 449.5±67.808, 60min: 184.8±35.989 e 300min:
151,0±10,583. Dos grupos que receberam hormônios, o grupo insulina+cortisol obteve melhor
(p<0,05) recuperação da glicemia (0min: 476.6±30.705, 60min: 174.3±39.716 e 300min:
211.4±34.158). No grupo insulina+cortisol+GDP 400mg/kg piorou (p<0,05) a recuperação da
glicemia (0min: 473±46.765, 60min: 125.3±30.089 e 300min: 87±16.971); e no grupo GDP 1g/Kg
houve melhora mais acentuada (p<0,05) do quadro hipoglicêmico (0min: 469±26.166, 60min:
285±51.075 e 300min: 185±35.525).
09.037 - EFFECTS OF TRIBULUS TERRESTRIS EXTRACT ON TESTOSTERONE LEVELS AND SEMEN
QUALITY IN RATS
RAYZZA TOMAZ GRIEBELER; RAISSA FORGERINI; PAULO CORDEIRO; MARILÚ MARTINS GIOSO; JOSE
ANTONIO DIAS GARCIA; VITORIA LIMA BOAVENTURA; DIEGO FURTADO CUNHA; ESTER SIQUEIRA
CAIXETA
UNIFENAS, ALFENAS, MG, BRASIL.
Tribulus terrestris (TT) is an herb that has been used in the popular medicine to enhance sexual
and physical performance. This study was designed to investigate the effects of TT extract on
testosterone levels and semen quality of rats. Forty five male Wistar rats (Rattus norvegicus)
weighing 250±20g, were divided into three groups. Group I served as a control and groups II and
III were treated with two different doses of TT extract (50 and 150mg/kg, respectively), orally,
once daily for 60 days. At the end of treatment the animals were anesthetized and blood samples
were collected for measurement of serum testosterone concentration. Then, semen was collected
from the epididymis to analyse of motility (0-100%), vigor (0-5) and sperm concentration
(106sptz/ml). Data were expressed as mean±SEM, tested by ANOVA and groups were compared by
Tukey, with significance level of 5%. Treatment with TT at both doses, did not affect testosterone
levels. The percentage of motility increased in rats given high dose TT (73.07±3.27 b) compared to
control animals (54.4±5.04a), but both of the groups were not different from the low dose group
(58±5.87ab). Sperm concentration decreased in group treated with 150mg/kg TT (6.21 ± 1.39 b)
compared to control group (28.02±5.9 a), which did not differ from the group treated with 50mg/kg
(21.6±3.7ab). No effects of TT were observed in vigor. In conclusion, the TT extract in high doses
affect sperm parameters, however, has no effect on testosterone concentration.
09.038 - SEEING IS BELIEVING: HYPOTHERMIA IN HUMAN SEPSIS IS A REGULATED PHENOMENON
MONIQUE THAÍS COSTA FONSECA1; ABNER C RODRIGUES2; LUANA C CEZAR1; ANDRE FUJITA2;
FRANCISCO G SORIANO3; ALEXANDRE A STEINER1
1.INSTITUTE OF BIOMEDICAL SCIENCES, UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL;
2.INSTITUTE OF MATHEMATICS AND STATISTICS, UNIVERSITY OF SAO PAULO, SAO PAULO, SP,
BRASIL; 3.MEDICAL SCHOOL, UNIVERSITY OF SAO PAULO, SAO PAULO, SP, BRASIL.
Although septic patients are usually febrile, a considerable portion of them develops hypothermia.
Typically, hypothermia is a reason for concern in sepsis to the extent that septic patients who
become hypothermic are often subjected to forceful rewarming. This practice, however, is not
based on solid evidence. The present study was the first to evaluate the dynamics of hypothermia
in septic patients who were not subjected to rewarming. Records of 50 patients with body
temperatures ≤ 35.5ºC for at least 4 h were randomly selected from the 2006-2012 database of
the adult ICU of a Brazilian university hospital. These patients presented 138 episodes of
hypothermia, of which 136 were clearly transient and self-limiting. In 96% of the cases, body
temperature did not fall below 34.0ºC. The median duration of the hypothermic episodes was 8
h. The majority of the patients developed hypothermia in the first 24 hours of admission and
presented less than three episodes of hypothermia. The odds of developing hypothermia were not
modified by the presence of shock or respiratory distress. Interestingly, the prevalence of
hypothermia was lower at 2 h before death than at earlier time points. These data indicate that
hypothermia in human sepsis is a regulated, nonterminal phenomenon that occurs independently
of shock or respiratory distress. The need to act against hypothermia in sepsis should be
reevaluated in light of these findings.
09.039 - EFFECT OF GLIBENCLAMIDE THERAPY COMBINED WITH METFORMIN ON GLUCOSE
METABOLISM OF PREDIABETIC RATS
CARINA PREVIATE; ISABELA PEIXOTO MARTINS; FLÁVIO ANDRADE FRANCISCO; KELLY VALÉRIO
PRATES; ROSIANE APARECIDA MIRANDA; AUDREI PAVANELLO; VERIDIANA MOTA MOREIRA; THAUANY
FABRI CORÁ; VANDER SILVA ALVES; ELAINE VIEIRA; RODRIGO MELLO GOMES; PAULO CESAR DE
FREITAS MATHIAS; CLAUDINÉIA CONATIONI DA SILVA FRANCO
Background/Aims: Obesity is a major public health problem associated with increased risk of type
two diabetes mellitus (T2DM). Considering that patients with T2DM need of drug therapy to keep
the normoglycemia, the goal of current study was to characterize the action of glibenclamide
therapy combined with metformin on glucose metabolism of prediabetic rats.
Methods: Wistar rats were treated during the first 5-days of life with monosodium L-glutamate
(MSG) to induce pre-diabetes and controls received saline. At weaning, the rats were divided in
four experimental groups: Controls-Water (C-W), MSG-Water (MSG-W), Control- Glibenclamide
(2mg/kg) and Metformin (250mg/kg) (C-Gli+Met) and (MSG-Gli+Met) treated by gavage until 100day-old. After the treatment, the following parameters were analysed: final body weight (bw);
periepididymal adipose tissue; intravenous glucose tolerance test (ivGTT).
Results: MSG neonatal treatment induced obesity in adult rats. MSG-Gli+Met rats exibited lower
bw and periepididymal adipose tissue than C-W, C-Gli+Met and M-W groups. Glycemia was similar
in C-Gli+Met, MSG-Gli+Met and C-W rats, however, there was a 20 % reduction in glucose levels in
the MSG-Gli+Met rats compared to MSG-W rats.
Conclusions: Chronic double therapy with antidiabetic drugs reduces obesity in adults MSG-rats.
Key words: Obesity, monosodium L-glutamate, glibenclamide, metformin.
THEME 10 - PHYSIOLOGY EDUCATION
10.008 - ASSESSMENT OF LEARNING BEFORE AND AFTER THE USE OF A PUZZLE ABOUT CARDIAC
CYCLE
LAIS TONO CARDOZO; ALINE SOARES MIRANDA; FERNANDA KLEIN MARCONDES
FOP/UNICAMP, PIRACICABA, SP, BRASIL.
Educational games are useful strategies to promote an active student participation in the
teaching-learning process. In a previous study, we developed a puzzle to cardiac cycle education,
which was rated as helpful by students to their learning, but its effect was not analyzed on student
performance. The aim of this study was to evaluate if the use of the cardiac cycle puzzle improves
the performance in the assessment on the cardiac physiology. This project was approved by CEPFOP (protocol 34/2015) and included the participation of 65 undergraduate dental students from
Piracicaba Dental School, who attended to a 50 min lecture on cardiac physiology. At the end of
this lecture, the students were asked to study this subject, and were informed that that in the
next class an assessment would be applied. At the beginning of the second class, the evaluation
was applied, and was followed by an activity using the cardiac cycle puzzle. Early in the third
class, the same assessment was applied and the performance of students were compared with
achieved in the assessment performed before the activity with the game. The comparison was
made by paired Student t test, considering p<0.05. Before the educational game, the concept of
the students (5.59 ± 0.22) was significantly lower than after the use of the game (6.65 ± 0.25),
representing a 19% increase. These data indicate an improvement in learning with the use of the
cardiac cycle puzzle.
10.009 - THE USE OF WORKSHOPS TO MOTIVATE THE INTERESTING BY PHYSIOLOGY
LARISSA DE MATOS SOUZA; SIMONE SOUZA DE OLIVEIRA; CLÁUDIA ELENA CARNEIRO; ARISTEU VIEIRA
DA SILVA; ÉRICA MARIA GRANJEIRO
STATE UNIVERSITY OF FEIRA DE SANTANA, FEIRA DE SANTANA, BA, BRASIL.
This study reports on an experience in health education involving physiology teaching led by
students of health area courses at Feira de Santana State University. Workshops were used to
motivate the interesting by physiology. The workshops involved participation of Extension
Programs of State University of Feira de Santana, including Initiation to Teaching Institutional
Scholarship Program (PIBID) and New Talent Program. Both programs supported by Coordination
of Improvement of Higher Education Personnel of Education Ministry, BRAZIL, involve the
utilization of teaching innovate approaches and the communication between the universities and
publics basics schools. The physiology workshops were performed in the Feira de Santana State
University campus of Feira de Santana, state Bahia/Brazil during the National Science and
Technology Week in the year 2013 and 2014 and Brain Awareness Week in the year of 2014. Several
30
activities were proposed directed to prevention of cardiovascular, respiratory and nervous systems
diseases, respectively. The workshops by a participatory educational process provided to the
action of the interdisciplinary work, favoring the health promotion activities having the school as
a space of the basic attention.
10.010 - PHYSIOLOGICAL UNDERSTANDING WEEK 2014: THE IMPACT OF ACTIVITIES PERFORMED
IN URUGUAIANA/RS/BRAZIL
JEFFERSON ROSA DE MENEZES1; PAMELA BILLIG MELLO CARPES1; CAROLINE DALLA COLLETTA
ALTERMANN2
1.UNIVERSIDADE FEDERAL DO PAMPA, URUGUAIANA, RS, BRASIL; 2.CAROLINE DALLA COLLETTA
ALTERMANN, URUGUAIANA, RS, BRASIL.
The Physiological Understanding (PhUn) Week is an American outreach program that aims building
connections between scientists and their local schools through the discussion of physiology
contents. Considering the importance of this proposal, we propose perform this project in a city
of Rio Grande do Sul/Brazil and evaluate the impact of the activities. The activities were held in
the days 03 to 07 of November 2014 (official period of Phun Week). There were performed
activities with children between 10 and 16 years-old, such as lectures, anatomy practices, simple
physiology experiments, like the measure of blood pressure and heart rate before and after of a
physical exercise perform. To evaluate the impact of actions, the students answered
questionnaires, including questions about their previous participation in activities like this, about
their interest in it, and about the important give to learn more about the human body. Finally,
we asked about how good they thought this activity. 302 students participated in the activities.
We verified that 92% considered important know about physiology and 75% deemed important the
information’s passed on activities for his everyday life. Finally, 96% judged the activities as good
or excellent. Observing the results we can affirm that the proposed of perform PhUn Week
activities in Uruguaiana promoted a great involvement of students, considering that they normally
did not have opportunities like, that can aggregate knowledge increase the interest of students
in physiology.
10.011 - EXPOSURE “JOURNEY TO CELL GIANT”: A TEACHING TOOL TO MOTIVATE THE
INTERESTING BY CELLULAR PHYSIOLOGY
LARISSA DE MATOS SOUZA1; CELSO DAMASCENO SILVA1; MAISE RIBEIRO ARAÚJO1; MICHELLE SILVA
DE SENA1; ANA LUCIA ALBUQUERQUE PEREIRA COSTA2; VIGNY SANTOS DA SILVA2; ÉRICA MARIA
GRANJEIRO2
1.STATE UNIVERSITY OF FEIRA DE SANTANA, FEIRA DE SANTANA, BA, BRASIL; 2.COLLEGE MODELO
LUÍS EDUARDO MAGALHÃES, FEIRA DE SANTANA, BA, BRASIL.
The teaching of physiology, now, constitutes a great concern in all of the teaching levels,
especially in the fundamental teaching. The classes are only in abstract explanations and the
students, in this phase, they still have difficulty in abstract terms. In that sense, biological
sciences students of Extension Program of State University of Feira de Santana, Initiation to
Teaching Institutional Scholarship Program (PIBID), proposed the exposure "Journey to Cell Giant",
which was developed and applied in the public school College Modelo Luís Eduardo Magalhães
(CMLEM), Feira de Santana/Bahia. The project was conducted in three main stages involving
students of basic and high school: first: students crafted cytoplasmic organelles, preferably in
recyclable material; second: scholars constructed a three-dimensional model of eukaryotic cells
(vegetable / animals); third: exposure to scholar community. In all stages, the participants studied
and discussed cellular physiology concepts related in the contents of the classes. The exposure
was visited by 98 students from the 1st grade of secondary education of CMLEM. After exposure,
was used the Likert Scale to measure the level of satisfaction of individuals. Thus, 70% of students
that visited the exposure related enthusiasm to study cellular physiology. These data show that
the use didactic models, interacting with the contents of the classes, may be an interesting
teaching tool to stimulate the interesting by cellular physiology.
10.012 - STUDENTS’ PERCEPTION ABOUT THE TEACHING METHODS USED IN A NURSING COURSE
ANDRÉIA DE CARVALHO ZANETTI1; LUANA CAROLINA APARECIDA ALMEIDA1; FERNANDA KLEIN
MARCONDES2; KELLY CRISTINA GAVIAO1
1.FACULDADE PITÁGORAS, POCOS DE CALDAS, MG, BRASIL; 2.FOP UNICAMP, PIRACICABA, SP,
BRASIL.
Active Methodology has been studied and used in many countries, as a way to stimulate students
to be active in the learning process. The aim of this study is to investigate the students’ perception
about the teaching methods used in a nursing course. A questionnaire was created and given to
the Pitagoras Poços de Caldas nursing course students (n=128). The questionnaire contains
questions about students’ perception of different methods that are applied by different teachers
in the course. In the first question, the students were oriented to choose the best method for
their own learning process. The second question asked students to evaluate, from 0 to 10, the
different methods of teaching. The first question results showed that the students prefer active
learning methods associated with traditional lectures (n=125), to traditional lectures only (n=1).
Two students nullified the issue. The second question results showed that students evaluated the
active methods as better than traditional lectures only. Practical classes received highest score
(9,7), followed in group discussions (8,5) and case studies (8,3), always associated with lecture
expositive. We can conclude that from the students point of view, the active methods associated
with traditional lectures, are better choices for the learning process than only traditional lectures.
10.013 - THE USE OF AN EDUCACIONAL GAME MAY INCREASE THE LEARNING ABOUT ACTION
POTENTION
KELLY CRISTINA GAVIAO1; FERNANDA KLEIN MARCONDES2
1.FACULDADE PITÁGORAS, POCOS DE CALDAS, MG, BRASIL; 2.FOP UNICAMP, PIRACICABA, SP,
BRASIL.
Action potential is a difficult concept that undergraduate students should learn. The aim of this
work was to evaluate if the use of an educational game could help students to learn this subject.
After a lecture about resting membrane potential, 65 dental students were requested to read
about resting membrane and action potential, for the next class. The students were divided in
two groups: control and experimental. Control group (n = 34) answered questions about action
potential in order to assess their knowledge acquired by reading the subject. Experimental group
(n= 31) performed an activity with an educational game. The game presents chips indicating the
ions sodium, potassium, chloride, ionic channels, Na+-K+- ATPase, intracellular and extracellular
medium and membrane. The students were asked to show how the ions are transported across the
membrane, by leak channels, voltage-gated channels, and Na+ - K+- ATPase during the resting
membrane potential and action potential. They were asked to indicate it in a figure of action
potential. At the end of the activity, the experimental group performed the assessment. Data
were compared by paired Student t test (p < 0.05). The score of experimental group was higher
(8.15 +/- 0.44) in comparison with control group (4.67 +/_ 0.31). The number of erroneous answers
were lower in experimental (1.87 +/_ 0.37) compared to control (4.88 +/_ 0.43) group. These
results indicate that the use of the educational game improved the learning about action
potential.
31
POSTER SESSIONS | TUESDAY | AUGUST 4
THEME 01. EXERCISE PHYSIOLOGY
01.040 - VALIDATION OF A TEMPERATURE SENSOR MADE IN BRAZIL FOR MEASUREMENT OF
MUSCLE TEMPERATURE IN HUMANS
YGOR ANTONIO TINOCO MARTINS; CHRISTIAN EMMANUEL TORRES CABIDO; GUSTAVO GUIMARÃES
AGUIAR DE OLIVEIRA; SARA RODRIGUES; BARBARA PESSALI MARQUES; LUCIANA PESCE CARVALHO;
SAMUEL PENNA WANNER; MAURO HELENO CHAGAS
UNIVERSIDADE FEDERAL DE MINAS GERAIS, CONSELHEIRO LAFAIETE, MG, BRASIL.
The measurement of muscle temperature extend the possibilities of research in thermoregulatory
studies in exercise physiology. The lack of use of this type of measurement in Brazil can be related
to high cost of the equipment imported. The aim of this study was to analyze the validation of
amuscle temperature sensor produced in Brazil. A muscle temperature sensor already
internationally validated (IT-18, Physitemp, Clifton, USA) was applied used as reference
to validate a muscle temperature sensor (Thermocouple Conventional Type “t”) produced in Brazil
(Exact Ind. LTD, Brazil). Both sensors were connected to the index temperature marker with an
accuracy of ± 0.1° C. An "Boiled water" with reciprocal agitation was used to modify the water
temperature (20 to 45 ° C every 1° C) in which the sensors were immersed, and it was also used
a mercury thermometer with an accuracy of ± 1° C. The significance level was set at α<5%. The
results also indicated significant, high and positive correlations (r = 1,00; p <0,001). For the
analysis of Bland Altman the limits of the found agreement varied from -0.16 to 0.03 ° C. In
conclusion, from this study made in Brazil, the acquisition of this National sensor can be used to
measure the muscle temperature. The above authors declare no conflict of interest for the
achievement of this study.
01.041 - THE CORRELATION BETWEEN THE THICKNESS OF THE SUBCUTANEOUS TISSUE AND THE
CHANGE IN MUSCLE TEMPERATURE SUBJECTS EXPOSED TO A COLD ENVIRONMENT
CHRISTIAN EMMANUEL TORRES CABIDO; GABRIEL RESENDE QUINAN; YGOR ANTONIO TINOCO
MARTINS; LUIS BITTAR; ALESSANDRO DANIEL COSTA MAIA; LUCAS DE CASTRO BOECHET; SARAH DA
GLÓRIA TELES BREDT; SAMUEL PENNA WANNER; MAURO HELENO CHAGAS
This study investigated the association between the thickness of the subcutaneous tissue (TST)
and the change in the muscle temperature (MT) of subjects exposed to a cold environment (10°C;
60min). For achieving this purpose, the TST and temperature of the right vastus lateralis (VL)
muscle of 6 male volunteers (age = 27.0 ± 3.3 years; body mass = 76.5 ± 11.5 kg: muscle thicknesses
= 26.2 ± 3.4 mm; TST = 6.6 ± 2.4 mm; average ± DP) were analyzed. An ultrasonography device
was used to measure the thickness of the VL, which was divided by two and then summed to TST;
this calculation allowed us to determine the depth at which the sensor was inserted. Next, the VL
area was sterilized and a cannula (18G) was inserted in the thigh of the subjects. The temperature
sensor was inserted in the muscle through the guide cannula and, after 30 minutes of rest, the
volunteers were directed to an environmental chamber, where they remained seated, wearing
only shorts, socks and sneakers. The Pearson’s correlation coefficient was applied to evaluate the
association level between TST and the change in MT (end - beginning of cold exposure). During
the cold exposure, MT was decreased by 33.70 ± 1.37 to 29.94 ± 1.80C. A high negative and
significant correlation was observed between the analyzed variables (r=-0.84, p<0.05). This result
confirm that a thick subcutaneous tissue avoids large changes in the muscular temperature coldexposed subjects.
01.042 - AEROBIC EXERCISE TRAINING PREVENTS ENDOTHELIAL DYSFUNCTION IN LDLR-/- MICE
NÁDIA FAGUNDES GARCIA1; CAMILA DE MORAES2; MARIA ANDRÉIA DELBIN3; ANGELINA ZANESCO1
1.UNIVERSIDADE ESTADUAL PAULISTA, RIO CLARO, SP, BRASIL; 2.UNIVERSIDADE DE SÃO PAULO,
RIBEIRÃO PRETO, SP, BRASIL; 3.UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL.
To investigate the effects of aerobic exercise training (AET) on vascular function of LDLr-/- mice.
Wild-type mice (WT-C57BL/6J) and LDLr-/- (KO) were divided: sedentary WT (WT), trained WT
(WT/Ex), sedentary KO (KO) and trained KO (KO/Ex). WT groups were fed with standard diet and
KO groups with high fat diet. The AEF was performed for 8 weeks, 5 days/week, 60 min/day at
50-60% of maximum capacity. Epididymal fat (EF), blood glucose, total cholesterol (TC) and
triglyceride (TG) were evaluated. The maximal response (Emax) was obtained from concentrationresponse curves to acetylcholine (ACh), sodium nitroprusside (SNP), phenylephrine (PE) and
thromboxane A2 analog (U46619). EF was 77% higher in the KO group compared to WT and AET
was effective to prevent EF increase. Blood glucose was 25% higher in KO group (161±11mg/dL)
compared to WT (121±5 mg/dL) as TC (KO 248±16mg/dL and WT 83±5 mg/dL) and TG levels (KO
142±11mg/dL and WT 74±8mg/dL). AET did not modify these parameters. Emax to ACh was lower
in the KO group (58±7%) compared to WT (82±7%) and this response was improved in both trained
groups (KO/Ex 85±3% and WT/Ex 92±3%). No differences were seen on Emax to SNP, PE or U46619.
The AET prevented endothelial dysfunction in KO mice even without changes in lipid profile.
01.043 - UMA SESSÃO DE EXERCÍCIOS RESISTIDOS INFLUENCIA A COGNIÇÃO E O PERFIL DE
HUMOR
MARCUS VINICIUS LUCIO DOS SANTOS1; RICARDO BOTTURA1; CAMILA MARIA DE MELO1; SERGIO
TUFIK1; MARCO TULIO DE MELLO2; HANNA KAREN MOREIRA ANTUNES 1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DE
MINAS GERAIS, SÃO PAULO, SP, BRASIL.
Introdução: Ao longo dos anos o exercício físico resistido (EFR) ganhou destaque devido os seus
efeitos nos aspectos psicobiológicos. Porém, duvidas ainda permanecem sobre as respostas
agudas. Objetivo: Investigar os efeitos de diferentes sessões de EFR no humor e na cognição.
Métodos: Treze jovens com média de idade 29,92±3,07 anos e índice de massa corpórea de
24,88±2,63kg/m2, foram submetidos a uma sessão aguda de EFR de força e de resistência muscular
com duração de 50 minutos e intervalo de 7 dias entre as sessões. Antes, imediatamente após
(IA), 30 e 60 minutos após o término das sessões, avaliou-se o humor pela Escala Visual Análoga
de Humor (VAMS) e Escala Subjetiva de Experiência em Exercício (SEES) e cognição pelo Sistema
de Testes de Viena (Reaction time). Resultados: Utilizou-se ANOVA two-way com post hoc de
Duncan com p<0,05. Na análise de humor, pelo teste de VAMS verificou-se redução da ansiedade
IA em ambos os grupos (força p=0,02; resistência p<0,01) em comparação com o basal. No teste
de SEES o grupo resistência apresentou aumento do distresse psicológico (p=0,02) e diminuição do
bem-estar positivo (p=0,03) IA em comparação com o basal. Em ambos os grupos (força p=0,04;
resistência p<0,01) a variável fadiga aumentou IA em comparação com o basal. Na análise de
cognição reaction time, a variável tempo motor reduziu imediatamente após (p<0,01) em
comparação com o basal no grupo força, não havendo diferença no grupo resistência. Conclusão:
O exercício de força parece gerar um efeito benéfico no humor e cognição quando comparado ao
exercício de resistência.
01.044 - AEROBIC EXERCISE CONTRIBUTES TO H2O2/VEGF SIGNALING IN THE RAT LUNG WITH
PULMONARY ARTERIAL HYPERTENSION
RAFAEL COLOMBO 1; RAFAELA SIQUEIRA1; ADRIANA CONZATTI1; BRUNA GAZZI GAZZI DE LIMA
SEOLIN1; TÂNIA REGINA GATELLI FERNANDES1; ALESSANDRA EIFLER GUERRA GODOY 2; ISNARD
ELMAN LITVIN2; JAIRO MONTEMOR DA SILVA3; PAULO JOSÉ FERREIRA TUCCI3; ALEX SANDER DA ROSA
ARAUJO1; ADRIANE BELLÓ-KLEIN1
1.UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL; 2.UNIVERSIDADE
DE CAXIAS DO SUL, CAXIAS DO SUL, RS, BRASIL; 3.UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO
PAULO, SP, BRASIL.
Aims
Pulmonary arterial hypertension (PAH) is characterized by pulmonary vascular remodeling and
right ventricle overload. Knowing that angiogenesis is a key factor involved in the decrease of
vascular resistance to blood flow, it was tested the hypothesis that aerobic exercise exert a
positive impact on the hydrogen peroxide (H2O2) and protein kinase B (Akt) in the lung
parenchyma.
Methods and Results
To study the aerobic exercise effects on lungs angiogenesis signaling, it was used Wistar rats that
received monocrotaline (MCT) (60 mg / kg i.p.) or the same volume of saline (0.9% NaCl i.p.). It
was noted an increase of H2O2 (43%) in PAH trained animals (TM) compared to its control (SM).
H2O2 showed a positive correlation (r = 0.77) with VEGF. In TM animals, VEGF was increased (4.7
fold) compared to SM. Both VEGF as angiopoietin-1 (Ang-1) showed positive staining on lungs
parenchyma of TM and SM. Glutathione peroxidase increased its activity in the TM group (49%)
compared to TC. Aerobic exercise increased the activity of peroxiredoxin (P < 0.05). The increase
in VEGF positively correlated with Akt phosphorylation (r = 0.73). The p-Akt was shown to be
increased in TM animals when compared to SM animals (2.5 fold). The change in fractional area,
fractional shortening and systolic tricuspid annular plane excursion showed improvement after
exercise training.
Conclusion
Aerobic exercise promotes H2O2/VEGF/p-Akt signaling for pulmonary physiological angiogenesis.
It is associated with an improvement on RV function, evaluated by echocardiography.
01.045 - STRENGTH TRAINING IMPROVED SKELETAL MUSCLE REPAIR
SAMUEL RODRIGUES LOURENÇO DE MORAIS1; ALEXANDRE GINEI GOYA1; WAGNER GARCEZ MELLO1;
VALDIR GOUVEIA GARCIA2; EDILSON ERVOLINO1; SANDRA HELENA PENHA OLIVEIRA1; RITA CÁSSIA
MENEGATI DORNELLES1
1.UNIVERSIDADE ESTADUAL PAULISTA, ARACATUBA, SP, BRASIL; 2.GROUP OF RESEARCH AND
STUDY ON LASER IN DENTISTRY (GEPLO), UNIV. ESTADUAL PAULISTA, ARAÇATUBA, SP, BRASIL.
Methods: Sixty Wistar rats were randomly assigned into 6 groups: (1) Non-exercised (NE), (2) NE
plus muscle lesion (NE+LE), (3) NE+LE plus LLLT (NE+LE+LLLT); (4) Strength training (ST), (5) ST+LE
and (6) ST+LE+LLL. ST was performed for 10 weeks and after that, cryolesion was induced in
tibialis anterior muscle. LLLT (660 nm) was administered during 14 days after cryolesion. Results:
ST animals showed more advanced and organized repair process, higher amount of CI (NE:
6.81±0.14 vs ST: 23.1±2.33), IL-10 (NE: 784.04± vs 1258.63±113.79) and IL-6 (NE: 1265.46±305.97),
and also better responsivity to LLLT treatment than in non-exercised animals. LLLT reduced
significantly the TNF-α level (P <0.05) and it was able to stimulate CI amount and maturation (P
<0.05). Conclusion: Animals that were submitted to ST showed better capacity to tissue repair
than in non-exercised animals and they were more responsive to LLLT treatment. LLLT was
effective in improved muscle repair and prevents against fibrosis in ST practitioners and it is
suggest being an effective therapeutic intervention on muscle repair. Key words: Skeletal muscle
repair; Low-level laser therapy; Cytokines; Strength training; Cryolesion
01.046 - EFFECT OF EXERCISE SESSIONS IN ROWING ERGOMETER AND ON THE BOAT IN THE
WATER STATUS OF ROWERS
JOSIE DE SOUZA OLIVEIRA CAMPOS; GABRIEL DIAS RODRIGUES; PEDRO PAULO DA SILVA SOARES
FEDERAL FLUMINENSE UNIVERSITY, NITEROI, RJ, BRASIL.
The rowers suffer great physiological stress during training, which associated with adverse
weather conditions can result in severe dehydration resulting in poor performance. This study
aimed to evaluate the water status of rowing athletes during exercise on rowing ergometer and
on boat. Data were collected in rowing clubs and on field in Rio de Janeiro. 14 rowers participated
(21.2 ± 3.9 years) male. We analyzed weight measurements at pre-exercise and post-exercise
(variation body mass - ΔBM), the rowing ergometer and on boat, and liquid ingested during the
sessions, with moderate to high intensity, in a single day. Despite the different climatic conditions
(temperature (oC) and relative humidity (RH)) between the session on boat (25.3°C ± 1,8oC and
75.0% ± 6.8% RH) and the rowing ergometer (21.6 ± 1,8 oC and 66.2 ± 3.4% RH) (oC p <0.001; RH
p <0.01), no difference (p> 0.05) in the dehydration caused by sessions. The ΔBM the ergometer
was 0.57 ± 0.40 (p <0.001) and the boat was 0,35kg ± 0.64 (p <0.001), representing 0.72% and 0.94
± 0.47 dehydration ± 0.68%, respectively. The relationship between fluid intake during the sessions
and the sweat rate (ml/ h) showed a positive and significant correlation only in the years in rowing
ergometer (r = 0.614; p <0.01). We conclude that both sessions in rowing ergometer as on boat
significantly reduced the BM after exercise causing changes in parameters related to the water
status of athletes.
01.047 - MODERATE AEROBIC EXERCISE DOES NOT PROVIDE BETTER GLYCEMIC CONTROL WHEN
IT IS ASSOCIATED WITH CHRONIC STRESS
ANDREA SANCHES1; RAFAELA COSTA2; MAELINE SANTOS MORAIS CARVALHO1; MARIA FERNANDA DA
SILVA ANDRADE1; GIZELE BRUNA BARANCKEVICZ3; FERNANDA KLEIN MARCONDES1; TATIANA DE
SOUSA DA CUNHA4
1.PIRACICABA DENTAL SCHOOL, FOP/UNICAMP, PIRACICABA, SP, BRASIL; 2.INSTITUTE OF
BIOLOGY, IB/UNICAMP, CAMPINAS, SP, BRASIL; 3.UNIVERSITY OF SÃO PAULO/ESALQ, PIRACICABA,
SP, BRASIL; 4.SCIENCE AND TECHNOLOGY INSTITUTE, SÃO JOSÉ DOS CAMPOS, SP, BRASIL.
We have previously shown that chronic mild and unpredictable stress (CMS) induced endothelium
dysfunction and higher area under the curve of oral glucose tolerance test (OGTT) in rats. On the
other hand, physical exercise is recognized to provide better glycemic control. Thus, the aim of
this study was to evaluate the effect of moderate exercise on the response to OGTT of rats
submitted to CMS. Male Sprague-Dawley rats were divided into four groups: 1)Control, 2)Exercise,
3)CMS and 4)Exercise plus CMS (n=7-9/group). CMS protocol consisted on application of different
stressors, 7 days/wk during 3 wk (5, 6 and 7th wk of protocol). The physical exercise was
performed after a period of adaption on the treadmill and consisted of an intensity of 50–70% of
the maximal exercise test, 5 days/wk, and 1 hour/day (during 8 wk). Data were analyzed by
ANOVA bifatorial (p<0.05). All procedures were approved by Institutional Ethics Committee.
Exercise, CMS and Exercise plus CMS presented higher corticosterone plasma level in comparison
with Control group (p˂0.05). Exercise (781.1 ± 17,2 mmol x min/L) and Control (842.9 ± 15.5 mmol
x min/L) group presented lower area under the curve of glucose during OGTT in comparison with
Exercise plus CMS (872.0 ± 12,9 mmol x min/L) and CMS (879.5 ± 11.5 mmol x min/L) group,
respectively (p˂0.05). These data show that aerobic training has not been able to abolish the
impairment of glucose tolerance induced by CMS.
01.048 - AEROBIC TRAINING RESTORES CIRCULATING ENDOTHELIAL PROGENITOR CELLS
FUNCTION IN INTRAUTERINE GROWTH RESTRICTED RATS – POSSIBLE ROLE IN THE ENDOTHELIAL
FUNCTION
VANESSA OLIVEIRA1; LIVIA VICTORINO DE SOUZA1; SEBASTIÃO DONATO SILVA JUNIOR2; TIAGO
FERNANDES2; MARIA HELENA CATELLI DE CARVALHO2; ELIANA HIROMI AKAMINE2; LISETE COMPAGNO
MICHELINI2; EDILAMAR MENEZES OLIVEIRA2; MARIA DO CARMO FRANCO1
1.FEDERAL UNIVERSITY OF S?O PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSITY OF SÃO PAULO, SAO
PAULO, SP, BRASIL.
Purpose: To evaluate the effects of intrauterine growth restriction (IUGR) and aerobic training
(AT) on circulating endothelial progenitor cells (EPCs) and endothelial functions.
Methods: Pregnant Wistar Rats were submitted to Ad libitum or 50% of Ad libitum diet during
gestation. At birth, pups were weighted. Adult Male offspring of both groups were assigned to
Sedentary Control (SC), Sedentary Restricted (SR), Trained Control (TC) and Trained Restricted
(TR). After AT (10 weeks,5 days/week,1h/day,50-60% of maximum capacity) and heart rate (HR)
was recorded and the EPCs function was investigated in vitro by the colony-forming unit assays in
EPCs from peripheral blood. The vasodilatation endothelium-dependent were assessed by
concentration-response curves to Acetylcholine (ACh:10-9M-3X10-5M). Results as mean ± SEM.
Results: IUGR was confirmed (Control:7.01±0.34gvs.Restricted:4.3±0.08g). AT-induced reduction
in HR (SC:355±13bpm;TC:321±4 bpm;SR:343±4 bpm;TR:309±7 bpm). AT restored both the colony
number of EPCs (SC:7.75±0.97;TC:7.40±1.30; SR:3.80±0.82;TR:6.13±0.77), and the relaxation to
ACh (SC:92.18±2.7; TC:90.05±2.04;SR:65.04±4.4;TR:93.35±1.7) in aorta from IUGR rats.
Conclusion: Our results indicated that IUGR-induced reduction in EPCs function and endothelial
dysfunction in adult rats. However, AT restored both EPCs and endothelial functions in IUGR
animals. So, considering that EPCs could participate in endothelial function maintenance, we
speculated that the increase in EPCs function can contribute, in part, to normal endothelial
function observed in IUGR trained group
01.049 - EFFECT OF DIFFERENT PRE-EXERCISE PRACTICES ON BLOOD PRESSURE AND
PERFORMANCE
WALLACE MARTINS VIANNA RIBEIRO; RAONI DA CONCEIÇÃO DOS SANTOS; CESAR RAFAEL MARINS
COSTA; WELINGTON VILLELA DE PAULA; IGGOR TADDEU BAHIENSE FERNANDEZ; ANDERSON LUIZ
BEZERRA DA SILVEIRA
FEDERAL RURAL UNIVERSITY OF RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL.
Stretching has been widely used in sports as part of warm-up. However scientific studies showed
that muscle stretching may cause negative effects on several physiological responses such as a
decrease of strength, reaction time, balance, speed and resistance. The purpose of this study was
to compare the effect of the ballistic stretching (BS), static stretching (SS) and warm up (WU) on
blood pressure and performance.
The groups control (CT), SS, BS and WU underwent a maximal incremental exercise test on a cycle
ergometer. Systolic and diastolic blood pressure were measured during the entire experiment and
for 30 minutes of recovery. Mean blood pressure and pulse pressure were derived from
mathematical equations.
All data is described as mean ± standard deviation. Systolic blood pressure increased significantly
after BS (CT=114.3 ± 6.9; SS=121.9 ± 13; BS=137.3 ± 12.8; WU=122.2 ± 13.2; p<0.05). After maximal
test the subjects previously submitted to SS or WU showed significantly lower blood pressure than
CT (CT=155.7 ± 12.7; SS=140.7 ± 17.9; BS=150.4 ± 21.9; WU=144.1 ± 14.1; p<0.05) and no
differences were found during recovery. No significant differences were found on diastolic blood
pressure and total test time (p>0.05).
All pre-exercise activities applied in this study failed to improve performance or inducing postexercise hypotension. However, subjects that used SS or WU previously had a smaller increase in
blood pressure, which could be important in populations with cardiovascular impairments.
01.050 - EFEITO DO TREINAMENTO DOS MÚSCULOS INSPIRATÓRIOS NO CONTROLE AUTONÕMICO
CARDÍACO DURANTE A MANOBRA DE VALSALVA EM INDIVÍDUOS SAUDÁVEIS
THIAGO RODRIGUES GONÇALVES; GABRIEL DIAS RODRIGUES; PEDRO PAULO DA SILVA SOARES
Em indivíduos saudáveis é sabido que o treinamento dos músculos inspiratórios (TMI) pode
aumentar a força dos MI, porém seu efeito no controle autonômico cardíaco não está claro.
Avaliou-se o efeito do TMI na variabilidade da frequência cardíaca (VFC) e sensibilidade
barorreflexa (SBR) em repouso e durante a manobra de Valsalva (MV) em indivíduos saudáveis. 11
homens (27 ± 3 anos), antes e após o TMI, realizaram o registro da frequência cardíaca (FC) e da
pressão arterial (PA) na posição sentada (10min) com respiração controlada (0,25Hz) e, em
seguida, a MV. Avaliou-se a VFC pelo RMSSD, componentes de baixa (BF) e alta (AF) frequência do
espectro de potência da FC. Determinou-se a SBR pelo alfa de BF (αBF) e na MV pelo “overshoot”
na Fase IV a partir de regressão linear da PA sistólica (PAS) com intervalo de pulso. O TMI foi
realizado durante 1 mês a 60% da pressão inspiratória máxima (PIM). Pós-TMI, a força dos MI (138,9
± 15,3 vs 172,1 ± 23,4cm/H2O), o RMSSD (45,1 ± 26,3 vs 58,1 ± 31,2ms), o ln-AF (6,5 ± 1,1 vs 7,1
± 0,9ms2) e o αBF (9,0 ± 2,2 vs 10,9 ± 3,0 ms.mmHg-1) aumentaram em repouso (p˂0,05). O TMI
diminuiu (p˂0,05) a taquicardia (∆25,7 ± 11,4 vs ∆19,6 ± 10,6bpm; fase II) e a braquicardia (-∆40,4
± 20,5 vs -∆28,2 ± 24,6bpm; fase IV) sem alterar a dinâmica da PAS durante MV (p>0,05). Não
houve diferença entre as retas de regressão pré e pós-TMI (p>0,05). O TMI melhorou a VFC e a SBR
em repouso e atenuou a dinâmica da FC durante a MV.
01.051 - O GANHO DE FORÇA INSPIRATÓRIA É MAIOR EM INDIVÍDUOS COM BAIXA PRESSÃO
INSPIRATÓRIA MÁXIMA APÓS TREINAMENTO ESPECÍFICO
IGOR VALERIANO PEREIRA DOS SANTOS BARRETO; GABRIEL DIAS RODRIGUES; ROGÉRIO BARBOSA
MAGALHÃES BARRO; THIAGO RODRIGUES GONÇALVES; PEDRO PAULO DA SILVA SOARES
FLUMINENSE FEDERAL UNIVERSITY, NITEROI, RJ, BRASIL.
Introdução: O treinamento da musculatura inspiratória (TMI) é utilizado para aumentar força dos
músculos inspiratórios. No entanto, não está claro como se dá o ganho (Δ) de pressão inspiratória
máxima (PIM) em indivíduos com PIM elevada (atletas) e não atletas. Portanto, o estudo avaliou
a PIM e o ∆PIM entre jovens saudáveis atletas e não-atletas após 4 semanas de TMI. Métodos:
Avaliamos 13 jovens saudáveis divididos em atletas (n=6) e não-atletas (n=7). Realizou-se
manovacuometria (Globalmed, BR) para determinar a PIM pré-TMI (PIMpré). A partir de 60%PIM os
voluntários realizaram o TMI por 4 semanas com 2 sessões diárias de 30 repetições em um resistor
inspiratório (Powerbreathe, UK). As cargas foram reajustadas semanalmente a partir de uma nova
PIM até a 4ª semana para determinar a PIM pós-TMI (PIMpós) O ganho de PIM (∆PIM) foi obtido pela
diferença de PIMpré e a PIMpós. Resultados: O grupo de não-atletas apresentou ∆PIM maior (38,0
± 3,87) do que os atletas (26,0 ±1,3; p= 0,03). No entanto, pré-TMI a PIM (cm.H2O) foi maior para
atletas (178,0 ±25,9) do que os não-atletas (121,3 ± 26,85; p= 0,04), o mesmo foi encontrado
nopós-TMI entre atletas (204,8 ± 27,0) e não-atletas (162,0 ± 22,98, ; p=0,03). Além disso, foi
encontrada correlação negativa entre o ∆PIM e a PIM inicial para todos os indivíduos (r= -0,56 e
p=0,04). Conclusão: Atletas apresentam maior PIM antes e após o TMI, comparados ao grupo
sedentário. No entanto, o maior ganho de PIM é observado em não-atletas.
01.052 - EFEITOS DO TREINAMENTO FÍSICO NO CURSO TEMPORAL DAS DISFUNÇÔES
OBSERVADAS EM MODELO DE SÍNDROME METABÓLICA
NATHALIA BERNARDES1; FILIPE FERNANDES CONTI2; DANIELLE DA SILVA DIAS2; JANAINA DE OLIVEIRA
BRITO2; Mª CLAUDIA IRIGOYEN1; KATIA DE ANGELIS2
1.UNIVERSIDADE DE SAO PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE NOVE DE JULHO, SAO
PAULO, SP, BRASIL.
O objetivo deste estudo foi avaliar os efeitos do treinamento físico (TF) no curso temporal das
alterações cardiometabólicas em ratos hipertensos (SHR) submetidos à sobrecarga de frutose.
Ratos machos Wistar e SHR foram divididos em grupos (n=6/grupo/tempo): Controle (C),
Hipertenso (H), Hipertenso+Frutose (HF) e Hipertenso+Frutose+TF (HFT). A sobrecarga de frutose
(100g/L) foi iniciada aos 30 dias de vida e as avaliações foram realizadas em 37, 45, 60 e 90 dias
de vida. O TF foi realizado em esteira (1h/d, 5d/sem, 8 sem). Sinais de pressão arterial (PA) foram
registrados através de um sistema de aquisição. A sensibilidade baroreflexa foi avaliada pelas
respostas taquicárdicas (RT) e bradicárdicas (RB). O grupo H apresentou menor RB e RT em 60 e
90 dias vs. o grupo C; a frutose induziu uma redução da RB em todos os tempos vs. o grupo C (37
e 90 dias: HF: -0,67±0,09 e -0,77±0,07 vs. C: -1,13±0,05 e -1,15±0,1; H: -1,01±0,04 e -0,80±0,05
bpm/mmHg); o TF foi capaz de atenuar essa disfunção em todos os tempos avaliados. Os grupos
H e HF apresentaram um aumento da PA em relação ao grupo C. A frutose induziu um aumento
adicional da PA em 90 dias, o que não foi observado no grupo HFT. O grupo HFT apresentou uma
redução da FC em 60 (11%) e 90 (7%) dias vs. grupo HF. Não houve alterações nos triglicérides e
glicose sanguíneos nos tempos avaliados. Concluindo, a disfunção do barorreflexo precedeu as
alterações hemodinâmicas e metabólicas neste modelo de síndrome metabólica e o TF foi eficaz
em atenuar as disfunções autonômicas e hemodinâmicas.
32
01.053 - TREINAMENTO FÍSICO DE ALTA INTENSIDADE PREVINE ALTERAÇÕES CARDÍACAS,
AUTONÔMICAS PERIFÉRICAS E INFLAMATÓRIAS EM RATOS INFARTADOS
NICOLLE MARTINS CARROZZI1; GABRIEL INÁCIO HONORATO1; JULIANA CRISTINA OLIVEIRA1; JANAINA
DE OLIVEIRA BRITO1; LUIZA MELANIE SILVA1; CATARINA DE ANDRADE BARBOZA1; DANIELE JARDIM
FERIANI1; MARCOS ELIAS ABSSAMRA1; LEANDRO YANASE ROCHA1; LILA MISSAE OYAMA2; BRUNO
RODRIGUES3
1.UNIVERSIDADE SÃO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE FEDERAL DE SÃO
PAULO, SAO PAULO, SP, BRASIL; 3.FACULDADE DE EDUCAÇÃO FÍSICA, UNIVERSIDADE ESTADUAL DE
CAMPINAS, CAMPINAS, SP, BRASIL.
Foram avaliadas as respostas ventriculares, autonômicas e inflamatórias do infarto do miocárdio
(IM) em ratos previamente submetidos ao treinamento físico (TF) aeróbico de alta intensidade.
Ratos Wistar machos foram divididos em (n=9 por grupo): controle (C), sedentário infartado (I) e
treinado em alta intensidade (90-95% da velocidade máxima do teste de esforço) infartado (TI).
Após o TF/acompanhamento, os animais foram submetidos às avaliações ventriculares,
autonômicas e do perfil inflamatório da musculatura esquelética. O TF prévio preveniu disfunção
sistólica (fração de ejeção – I: 43,8±0,8 vs. SI: 54,6±0,3) e diastólica no grupo treinado em relação
ao sedentário. A modulação autonômica simpática vascular, representada pela banda de baixa
frequência da pressão arterial sistólica, foi atenuada pelo TF prévio. No músculo sóleo, as
concentrações de IL-6 e TNF-α estavam aumentadas no grupo I quando comparado com o C.
Entretanto, tal aumento foi prevenido no TI (IL-6: -67% e TNF-α: -63%) em relação ao I. Além disso,
a expressão proteica de TLR4 estava aumentada em 23% no grupo I em relação ao TI. Os resultados
sugerem que o TF aeróbico de alta intensidade preveniu alterações cardíacas e vasculares que
conduziram uma redução do perfil inflamatório do músculo esquelético de ratos treinados
previamente ao IM. Em adição, tal redução do perfil inflamatório pode estar associado à redução
da expressão proteica de TLR4 nos animais estudados.
01.054 - EFEITOS DO TREINAMENTO FÍSICO COMBINADO NAS ALTERAÇÕES VENTRICULARES
PROVOCADAS PELO INFARTO DO MIOCÁRDIO EM RATOS
JULIANA CRISTINA OLIVEIRA1; LEANDRO YANASE ROCHA1; NICOLLE MARTINS CARROZZI1; LUIZA
MELANIE SILVA1; CATARINA DE ANDRADE BARBOZA1; MARCOS ELIAS ABSSAMRA1; DANIELE JARDIM
FERIANI1; BRUNO RODRIGUES2
1.UNIVERSIDADE SÃO JUDAS TADEU, SAO PAULO, SP, BRASIL; 2.FACULDADE DE EDUCAÇÃO FÍSICA,
UNIVERSIDADE ESTADUAL DE CAMPINAS, CAMPINAS, SP, BRASIL.
O presente estudo objetivou avaliar os efeitos do treinamento físico combinado (TC) nas respostas
ventriculares de ratos submetidos ao infarto do miocárdio (IM). Ratos Wistar machos foram
divididos (n=10 cada): controle (C), IM (IS), IM + treinamento resistido (ITR), IM + treinamento
aeróbico (ITA) e IM + TC (3 dias de TA + 2 dias de TR). Após os protocolos de treinamento, os
grupos ITA e ITC apresentaram melhora da fração de ejeção, velocidade de encurtamento
circunferencial e relação E/A em relação ao grupo IS e ITR. A pressão arterial, reduzida no grupo
IS, foi normalizada pelos protocolos de treinamento. A frequência cardíaca, que estava aumentada
nos grupos IS e ITR em relação ao C (356±10 e 358±12 vs. 325±5 bpm), foi normalizada no ITA
(323±3 bpm); porém, uma redução adicional foi observada no grupo ITC (310±6 bpm). De forma
semelhante, o peso do tecido adiposo branco retroperitoneal foi reduzido nos grupos treinados,
no entanto o grupo ITC (1,3±0,1g) apresentaram redução adicional dessa variável se comparados
com os grupos IS (7,4±0,6g), ITA (3,3±1,1g) e ITR (4,6±1,3g). Os dados sugerem que o TC
proporcionou, além de alterações positivas ventriculares semelhantes ao TA, redução mais
acentuada do tecido adiposo branco. Em conjunto, esses achados sugerem que a associação do TA
ao TR pode ser uma ferramenta segura e mais eficaz no controle das alterações funcionais
cardíacas e da composição corporal provocadas pelo IM.
Palavras chave: Infarto do Miocárdio; Função ventricular; Tecido Adiposo; Treinamento Físico.
01.055 - COMBINED EXERCISE TRAINING ATTENUATES CARDIOMYOPATHY IN A MODEL OF
DIABETES AND MENOPAUSE
IRIS CALLADO SANCHES1; MORGANA BUZIN1; FILIPE FERNANDES CONTI1; DANIELLE DA SILVA DIAS1;
CHRISTIANE MALFITANO1; VERA SALEMI2; SUSANA LLESUY3; Mª CLAUDIA IRIGOYEN2; KATIA DE
ANGELIS1
1.NOVE DE JULHO UNIVERSITY, SAO PAULO, SP, BRASIL; 2.UNIVERSITY OF SÃO PAULO, SAO PAULO,
SP, BRASIL; 3.UNIVERSIDAD DE BUENOS AIRES, BUENOS AIRES, ARGENTINA.
Effective therapeutic tools to treat cardiomyopathy are urgently needed to reduce the
morbimortality of diabetic population. This study aimed to evaluate the effects of combined
exercise training (CET) on cardiac function, oxidative stress and inflammation in diabetic
ovariectomized rats. Female Wistar rats were divided into 4 groups (n=8): euglycemic (E), diabetic
(streptozotocin, 50 mg/kg, iv) (D), diabetic ovariectomized (DO) and trained diabetic
ovariectomized (TDO). The CET was performed on a treadmill and in a ladder (8 wks, 40-60% max.
capacity). The left ventricle function was evaluated by echocardiography. Oxidative stress and
inflammatory were measured on cardiac tissue. The D and DO groups showed impaired systolic
and diastolic functions, as well as increased cardiac overload, evaluated by myocardial
performance index (MPI-D: 0.32±0.05; DO: 0.39±0.13 vs. E: 0.25±0.07) in relation to E group.
Systolic and MPI dysfunctions were exacerbated in DO group. The DO group presented higher
protein oxidation and lipoperoxidation than D groups. Glutathione redox ratio (GSH/GSSG) and IL10 were decreased in both D and DO groups when compared to E group. CET improved systolic and
diastolic functions, MPI (0.18±0.11), protein oxidation, lipoperoxidation, GSH/GSSG and IL-10 in
relation to the DO group. Concluding, CET was able to attenuate the cardiac dysfunctions,
probably by reducing inflammation and oxidative stress in an experimental model of diabetes and
menopause.
01.056 - CAN STATIC STRETCHING ALTER THE MANUAL REACTION TIME?
WELINGTON VILLELA DE PAULA; CESAR RAFAEL MARINS COSTA; RAONI DA CONCEIÇÃO DOS SANTOS;
WALLACE MARTINS VIANNA RIBEIRO; ANDERSON LUIZ BEZERRA DA SILVEIRA
Static muscle stretching is commonly used prior to sports. Scientific literature shows stretchingrelated impairments to several physiological variables. However, there is a lack of scientific
evidence about the influence of stretch on reaction time. The objective of this study was to assess
if different static stretching volumes can change the manual reaction time in young untrained
women. The sample was composed by 18 women (age= 24±5 yr), and they were submitted to three
experimental procedures: control (CTRL) in which there was no preparation for the test; 1 set (SS1) or 4 sets (SS-4) with 30 seconds of passive static stretching of the wrist joint. After each
procedure the subjects were submitted to the hand grip test. Moreover, the reaction time was
evaluated through electromyography signals, which they were collected by foam electrodes fixed
on the medial portion of the forearm, processsed by Spikerbox hardware and filtered and analyzed
through the softwares Audacity and Bioproc3. The electromyography evaluated signals were: total
reaction (TRT), pre-motor (PMT) and motor (MT). There was a significant increase for MT (CTRL=
914,8 ± 279,2; SS-1= 1520 ± 504,6; SS-4 = 1558 ± 606,9; p< 0,001) and TRT (CTRL= 996,8 ± 197; SS1= 1233 ± 271,1; SS-4= 1226 ± 297,4; p<0,01) in SS-1 and SS-4 when compared to CTRL; while PMT
did not change. The results show that different muscle stretching volumes can change negatively
hand reaction time, which could promote impairment on performance of several sports activity.
01.057 - INTERFERENCE OF DIFFERENT PASSIVE STATIC STRETCHING VOLUMES ON HAND
DEXTERITY
WELINGTON VILLELA DE PAULA; CESAR RAFAEL MARINS COSTA; RAONI DA CONCEIÇÃO DOS SANTOS;
WALLACE MARTINS VIANNA RIBEIRO; IGGOR TADDEU BAHIENSE FERNANDEZ; ANDERSON LUIZ
BEZERRA DA SILVEIRA
Hand dexterity is characterized as the capacity to move small objects in a specific task and it is
fundamental in sports. Static muscle stretching is commonly used as part of warm-up, however
scientific studies observed that this practice impairs several physiological variables, its effects on
dexterity, however, are inconclusive. This study aimed to evaluate if different volumes of static
stretch influence hand dexterity of young untrained women. The sample was composed by 18
women (age= 24±5 yr), and they were submitted to three experimental procedures: control (CTRL)
in which there was no preparation for the test; 1 (SS-1) or 4 (SS-4) sessions of stretching for the
muscles of shoulder, elbow and wrist. In each session the evaluator stretched the muscle to
threshold of pain or discomfort, and maintained this position for 30 seconds, with 30 seconds of
rest between sessions. After the procedure the individuals were submitted to manual dexterity
test. The time spent to perform Placing (CTRL= 6.4908 ± 1.099; SS-1= 7.580 ± 1.955; SS-4 = 7.837
± 1.994; p< 0,01) and Turning test (CTRL= 6.892 ± 1.058; SS-1= 7.937 ± 1.6271,1; SS-4= 8.514 ±
1.569; p<0,001) increased significantly in SS-1 and SS-4 compared to CTRL. The results show that
different muscle stretching volumes can change negatively hand dexterity, which could promote
impairment on performance of several sports activity.
01.058 - ACUTE AND CHRONIC INFLUENCE OF INTENSITY EXERCISE ON INFLAMMATORY PROFILE
IN PATIENTS WITH HEART FAILURE
ANDERSON ZAMPIER ULBRICH1; VITOR GIATTE ANGARTEN2; THIAGO GOMES HECK3; EDSON LUIZ DA
SILVA4; PAULO IVO HOMEM DE BITTENCOURT JR5; TALES DE CARVALHO2
1.UNIVERSIDADE FEDERAL DO PIAUÍ, CURITIBA, PR, BRASIL; 2.UNIVERSIDADE DO ESTADO DE SANTA
CATARINA, FLORIANÓPOLIS, SC, BRASIL; 3.UNIJUI, IJUÍ, RS, BRASIL; 4.UNIVERSIDADE FEDERAL DO
SANTA CATARINA, FLORIANÓPOLIS, SC, BRASIL; 5.UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL,
PORTO ALEGRE, RS, BRASIL.
To investigate the therapeutic effect of acute and chronic physical exercise of different intensities
in the inflammatory profile in patients with Heart Failure (HF). A randomized controlled clinical
trial, developed over 12 weeks, with twenty-two male patients diagnosed with compensated HF
and ejection fraction less than 45%, mean age 53.8 ± 8 years, class NYHA II and III, randomized
into two groups: group undergoing exercise of moderate intensity (GMI), and interval high intensity
group (GAI). Both groups performed aerobic exercise on a treadmill three times a week for 60minute workout. Evaluations were made on four occasions: before and after the first exercise
session and before and after the last session. The circulating inflammatory markers were
determined by serum citokine (IL-1, IL-6, IL-10; TNF-α), vascular endothelial growth factor (VEGF)
measured by ELISA. The heat shock protein 70 kDa (HSP70) of mononuclear cells was assayed by
Western Bloting and by Elisa kit. The inflammatory signaling pathways there was a decrease of IL6 (p <0.001) for both groups, with the greatest drop in the GAI, as well as for TNF-α by comparing
the same time. In the exercise session, the acute change happened in the last session for TNF-α,
VEGF and IL-10 in both groups. The GMI showed higher intracellular content of HSP70 with exercise
compared with GAI, and in the extracellular level of exports, both groups showed the same
response. In both groups was observed enhancement of the anti-inflammatory and cytoprotective
exercise, acute and chronic.
THEME 02 - RENAL PHYSIOLOGY
02.017 - INSULIN PREVENTS NHE3 STIMULATION IN EARLY STAGE OF DIABETES BUT HAS NO
EFFECT ON NHE3 INHIBITION IN LATE STAGE
RENAN GOIS MATEUS; ANGÉLICA PONTE DE AGUIAR; LARISSA MARIA ARAUJO SILVA; NÁDIA OSORIO
DE OLIVEIRA; NILBERTO ROBSON FALCÃO DO NASCIMENTO; MANASSES CLAUDINO FONTELES;
LUCÍLIA MA LESSA
UNIVERSIDADE ESTADUAL DO CEARÁ, FORTALEZA, CE, BRASIL.
Diabetic nephropathy is the most common cause of chronic kidney failure and end stage renal
disease. Several studies have suggested that NHE3 is enhanced in diabetes, contributing to the
expansion volume in the early stages of diabetes. However, changes in the exchanger activity in
later stages of diabetic nephropathy, are not well described. This study aimed to investigate the
changes in NHE3 activity in early stage (2 weeks) and late stage (8 weeks) of diabetes mellitus,
and the effects of treatment with insulin, in renal proximal tubules of STZ-diabetic rats, by
stationary renal microperfusion method. By this technique, we are able to measure the hydrogen
secretion in vivo. In 2 w-diabetic rats there was an increase in proximal bicarbonate reabsorption
when compared to its euglycemic control(JHCO3-,nmol.cm-2.s-1–2,18±0,06 contro l(n=6) x
2,86±0,07 2w-diabetes(n=6) P<0,05). Insulin treatment prevented the elevation of bicarbonate
reabsorption in 2 w-diabetic rats. However, in 8 w-diabetic rats there was an inhibition of proximal
bicarbonate reabsorption when compared to their euglycemic controls (JHCO3-,nmol.cm-2.s-1–
2,22±0,12 control (n=9) x 1,20±0,07 8w-diabetes (n=10). Insulin treatment of 8 w-diabetic rats did
not prevent this inhibitory effect. In the early stages of diabetes mellitus there is an increased
activity of NHE3, probably in dependence of hyperglycemia. Nevertheless, in the advanced stages
of diabetic nephropathy occurs inhibition of the exchanger.
02.018 - CHRONIC ETHANOL CONSUMPTION INCREASES SUSCEPTIBILITY TO SEPSIS: EVALUATION
OF RENAL DISORDERS
STHEFANY TEODORO RICCI; GABRIEL TAVARES VALE; CARLOS RENATO TIRAPELLI; CARLA SPERONI
CERON
Chronic ethanol consumption is associated with renal oxidative stress. Sepsis can cause renal
impairment leading to the development of acute renal failure by different mechanisms, such as
increased oxidative stress. The aim of this study is to investigate whether chronic ethanol
consumption-induced oxidative stress may increase susceptibility to sepsis. Male mice were
divided into 4 groups: control (C), ethanol 20% (v/v) 9 weeks (E), control+sespsis (CS) and
ethanol+sepsis (ES). Seven days after mild sepsis induction, a survival curve was obtained to
evaluate the mortality of animals. 24 hours after induction of sepsis animals were sacrificed and
renal tissue (cortex) was used for determination of TBARS, enzymatic and non-enzymatic
antioxidant activity and Western immunoblotting for Nox1 and Nox4. (CEUA Protocol:
13.1.1381.53.3). One-way ANOVA followed by Newman Keuls test (p<0.05). Ethanol consumption
decreases the survival rate (%) of animals subjected to sepsis (CS:100%; ES:60%). Ethanol causes
an increase in TBARS (nmol/mg protein) in control animals and subjected to sepsis (C:4.9±1.0,
n=13; E:8.4±0.8*, n=11; CS:6.9±0.6, n=11; ES:8.3±1.4*, n=9). Ethanol causes an increase in renal
GSH levels (µg/mg protein) in control animals and subjected to sepsis (C:10.3±0.3, n=10;
E:12.8±0.9*, n=8; CS:10.7±0.9, n=10; ES:14.7±0.7, n=10*). There was no difference in superoxide
dismutase activity (% inhibition) as well as protein expression of Nox1 and Nox4 in renal tissue.
Chronic ethanol consumption causes renal oxidative stress associated with increased susceptibility
to sepsis.
02.019 - ANÁLISE ULTRASSONOGRÁFICA RENAL E CARDÍACA EM RATOS SUBMETIDOS A
TREINAMENTO AERÓBIO MODERADO PRÉVIO À INJÚRIA RENAL AGUDA MEDICAMENTOSA
MARIA A RIBEIRO VIEIRA; LEDA MARIA DE CASTRO COIMBRA CAMPOS; ESDRAS GUEDES FONSECA;
ROBERTA DA SILVA FILHA; TATIANE CRISTINE SILVA ALMEIDA; MARIANA WERNERSBACH CHAGAS;
CAROLINA CAMPOS LIMA MOREIRA; LETÍCIA M SOUZA CORDEIRO; MARCOS BARROUIN MELO; ROBSON
AUGUSTO SOUZA DOS SANTOS
Os mecanismos pelos quais o exercício aeróbio moderado (EAM) pode conferir proteção renal ainda
necessitam ser elucidados. O objetivo deste estudo foi analisar a perfusão renal e a função
cardíaca em ratos submetidos a EAM prévio à IRA induzida pela gentamicina (GM). Ratos Wistar
machos foram divididos em 4 grupos (n=6-7): sedentários-salina (SED-SAL), SED-GM, EAM-SAL e
EAM-GM. Após uma semana de adaptação à esteira e gaiolas metabólicas, o EAM foi instituído por
8 semanas, previamente ao tratamento com GM (80 mg/kg/dia) ou NaCl 0,9%, via subcutânea, por
5 dias. Ao final do tratamento, foram realizadas ultrassonografia com Doppler das artérias renais
e ecocardiografia (ECO) e foram coletadas amostras de urina e sangue (CEUA/UFMG, 46/2014). A
creatinina sérica e proteinúria foram menores e o ritmo de filtração glomerular e osmolaridade
urinária foram maiores no grupo EAM-GM em relação ao grupo SED-GM (p <0,05). Não houve
diferenças significativas entre os grupos quanto aos índices de resistividade e pulsatilidade das
artérias renais. À ECO, os grupos GM apresentaram menores frações de ejeção e de encurtamento
que os grupos SAL (p <0,05). Entretanto, o treinamento instituído não provocou alterações
estruturais e funcionais cardíacas significativas no grupo EAM-GM em relação ao grupo SED-GM. Os
resultados indicam que o EAM foi capaz de atenuar a IRA induzida pela GM, mesmo sem alterar a
perfusão renal ou função cardíaca.
02.020 - TREINAMENTO AERÓBIO MODERADO ATENUA OS DANOS RENAIS INDUZIDOS PELA
GENTAMICINA EM RATOS
33
MARIA A RIBEIRO VIEIRA1; JULIANA BARROS ALMEIDA1; ESDRAS GUEDES FONSECA1; LETÍCIA M SOUZA
CORDEIRO1; TATIANE CRISTINE SILVA ALMEIDA1; ANDERSON JOSÉ FERREIRA1; MARCELO VIDIGAL
CALIARI1; LEDA MARIA DE CASTRO COIMBRA CAMPOS1; MARKUS DE OLIVEIRA BERGER2
FEDERAL DO RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL.
Embora os efeitos benéficos do exercício sobre o sistema cardiovascular sejam conhecidos,
existem poucos dados sobre os efeitos do exercício na função e estrutura renais. Neste estudo,
investigou-se o efeito do exercício aeróbio moderado (EAM) durante a injúria renal aguda (IRA)
induzida pela gentamicina (GM) (CEUA/UFMG, 177/2008). Ratos Wistar machos foram submetidos
a uma semana de adaptação e divididos em 4 grupos: sedentários: salina (SED-SAL), SED-GM, EAMSAL e EAM-GM. Durante a primeira semana, os ratos foram adaptados à esteira e às gaiolas
metabólicas individuais. Na segunda semana, o EAM foi iniciado (65% da velocidade máxima de
corrida, 30 min/dia, 3 dias/semana, durante 4 semanas) concomitantemente com o tratamento
com GM (80 mg/ kg/dia, subcutâneo, por 5 dias) ou NaCl 0,9%) nos respectivos grupos. Os grupos
SED também tiveram acesso a esteira (5m/min, 5 min/dia). Amostras de urina e sangue foram
recolhidas ao final de cada semana e os rins foram removidos ao final do experimento. No grupo
EAM-GM, a creatinina sérica (mg/dl) foi mais baixa (0,54 ± 0,09 vs 1,08 ± 0,15, SED-GM, n = 8, p
<0,05) e a taxa de filtração glomerular (Litros/24 h) foi maior no grupo EAM-GM (1,36 ± 0,22 vs
0,57 ± 0,16, SED-GM, n = 6, p <0,05). Além disso, na análise morfométrica, o grupo EAM-GM
apresentou menor extensão de infiltrado inflamatório e menor área tubulointersticial do que o
grupo SED-GM. Os dados sugerem que o EAM promove uma melhoria dos danos renais observadas
na IRA induzida pela GM.
02.021 - EFEITO DO HERBICIDA PARAQUAT E DO TRATAMENTO COM ANGIOTENSINA (1-7) SOBRE
A FUNÇÃO RENAL EM RATOS NORMAIS
ROBERTA DA SILVA FILHA1; ESDRAS GUEDES FONSECA1; LEDA MARIA DE CASTRO COIMBRA CAMPOS1;
MARIA DA GLÓRIA RODRIGUES MACHADO2; VIRGINIA SOARES LEMOS1; ROSÁRIA DIAS AIRES1; ROBSON
AUGUSTO SOUZA DOS SANTOS1; MARIA A RIBEIRO VIEIRA1
1.UFMG, BELO HORIZONTE, MG, BRASIL; 2.FACULDADE DE CIÊNCIAS MÉDICAS DE MINAS GERAIS,
BELO HORIZONTE, MG, BRASIL.
Neste estudo foi avaliado o efeito do paraquat (PQ), um potente herbicida, sobre a função renal
e o efeito da angiotensina(1-7) (Ang(1-7)) sobre os possíveis danos renais causados pelo PQ. Ratos
Wistar foram divididos em 4 grupos (n = 6/grupo): Grupos Controle (CTL) e CTL+Ang (1-7)
receberam NaCl 0,9%, intraperitoneal, ip); grupos PQ e PQ+Ang(1-7) receberam PQ (20mg/kg, ip).
Quatro horas após a injeção de PQ, os grupos CTL+Ang(1-7) e PQ+Ang(1-7) receberam Ang(1-7) (50
µg/kg, intragástrica). Após 24 h da injeção do PQ, os ratos foram anestesiados e, amostras de
sangue e urina foram coletadas e os rins foram removidos (CEUA/UFMG (85/2015). Os dados foram
analisados por TwoWay ANOVA seguida pelo teste de Bonferroni. O PQ, por si só, aumentou a
creatinina sérica (de 0,27 ± 0,02, CTL, para 0,48 ± 0,06 mg/dL, PQ) e a proteinúria (de 1,35±0,21,
CTL, para 7,58,1±0,70 mg/24h, PQ). A taxa de filtração glomerular (Litros/min) foi reduzida nos
grupos PQ (0,96±0,11) e PQ+Ang(1-7) (0,59±0,09) quando comparados ao grupo CTL (2,05±0,1)
(p<0,05). O PQ também aumentou o fluxo urinário (de 0,006±0,000 para 0,014±0,001 L/24 h). Em
resumo a Ang(1-7) não foi capaz de afetar significativamente as alterações induzidas pelo PQ.
Apenas no grupo PQ houve uma maior redução de peso corporal em relação ao grupo PQ+Ang(1-7,
p<0,05) . Os dados mostram que o PQ provoca danos agudos na função renal e que o tratamento
com a Ang(1-7) não foi capaz de atenuar/abolir tais danos.
02.022 - AVALIAÇÃO DOS PARÂMETROS FUNCIONAIS E ESTRUTURAIS RENAIS DE RATOS
TRATADOS COM NANOTUBOS DE CARBONO
TATIANE CRISTINE SILVA ALMEIDA; JULLIANE VASCONCELOS JOVIANO DOS SANTOS; VANESSA
BARBOSA ANDRADE; MARCOS AUGUSTO SÁ; LUIZ ORLANDO LADEIRA; MARIA A RIBEIRO VIEIRA;
ANDERSON JOSÉ FERREIRA
Estudos toxicológicos envolvendo possíveis efeitos dos nanotubos de carbono (NTC) nos sistemas
biológicos são cruciais para estabelecer a segurança desses materiais. Assim, o objetivo deste
estudo foi avaliar possíveis danos na função e estrutura renais de ratos cujos alvéolos dentários
foram tratados com NTC.Com o intuito de exacerbar algum possível efeito tóxico desses
compósitos, foi injetado intraperitonealmente (IP) concentrações maiores dos materiais. Foram
utilizados ratos Wistar divididos em 6 grupos: alvéolo dentário/controle (coágulo), NTCPS e
NTCPM (100µg/mL em 0,1mL) e intraperitoneal/controle (salina), NTCPS e NTCPM (0,75mg/kg).
Após 7 dias da administração dos materiais, os ratos foram sacrificados e amostras de sangue,
urina e o rins foram coletas.A análise da função renal dos ratos submetidos ao protocolo de alvéolo
dentário mostrou alterações significativas na concentração de creatinina plasmática (Controle:
0,27±0,04 ml/24h; NTCPM: 2554±0,057 ml/24h; ritmo de filtração glomerular (RFG)
(Controle:4546±676,6 ; NTCPM 2554±401,5 ml/24h ) p<0,05. IP: creatinina plasmática (Controle
0,26± 0,03; NTCPM 0,47± 0,047 ml/24h e RFG (Controle 1641 ±278,1; NCPM 762,5 ± 68,57 ml/24h);
fração de excreção de água (Controle: 0,7690±0,1368; NTCPS: 1,634±0,3356; NTCPM
1,5370±2774±ml/24h ) p<0,05. Esses resultados demonstram que os compósitos nas doses
utilizadas, podem alterar a função renal de ratos.
02.023 - LUMBAR, RENAL AND SPLANCHNIC SYMPATOEXCITATION IN A MODEL OF CHRONIC
KIDNEY DISEASE
GLAUCIA RAQUEL LUCIANO DA VEIGA; HEDER FRANK GIANOTTO ESTRELA; GISELE SILVERIO
LINCEVICIUS; GUIOMAR NASCIMENTO GOMES; ALEX YURI SIMÕES SATO; RUY RIBEIRO CAMPOS
JUNIOR; CASSIA TOLEDO BERGAMASCHI
UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL.
The renal sympathoexcitation and hypertension in chronic kidney disease (CKD) are already well
established. However, novel approaches for determining the extensions of this sympathetic
activation are necessary for better understand the mechanisms involved in CKD progression.
Nebivolol (NEB) is a cardioselective beta-blocker known to retard the progression of renal disease.
The aim of this study was to investigate the sympathetic activation for other regions and to
evaluate the effect of Nebivolol in sympatoexcitation and fibrosis after 4 weeks of orally
treatment with Nebivolol. CKD was induced in Male Wistar rats (250-300g) by 5/6 nephrectomy
surgery, the parameters: blood pressure (BP), heart rate (HR), splanchnic, lumbar and renal
sympathetic nerve activity (sSNA, lSNA and rSNA) and a renal fibrosis marker (collagen IV) were
assessed. It was observed a significant increase in BP in CKD (173±5, n=16 P<0.01) compared with
CTL (109±3 mmHg n=9). The rSNA was increased in CKD (158±12, n=16) vs CTL (96±5, n=9) as well
as lSNA (CTL 146±8 n=7; CKD 186±14, n=8) and sSNA (CTL 34±6, n=6; CKD 120±21 pps/s, n=6). The
renal fibrosis was increased in CKD (0.44±0.16 n=5) vs CTL (0.02±0.005%field, n=6). NEB treatment
did not change any of these parameters. These data suggest that CKD promotes
sympathoexcitation not only to the kidney but also to other organs that may contribute to
maintenance of hypertension and progression of renal failure. In addition, the β-blocker Nebivolol
was not able to improve either renal or cardiovascular dysfunction.
02.024 - RENAL HANDLING OF WATER AND ELECTROLYTES IN RESPONSE TO DISINHIBITION OF
THE PARAVENTRICULAR NUCLEUS OF HYPOTHALAMUS
ROBERTO BRAZ PONTES JR1; ERIKA EMY NISHI1; ADRIANA C GIRARDI 2; RUY RIBEIRO CAMPOS
JUNIOR1; CASSIA TOLEDO BERGAMASCHI1
1.UNIFESP, SAO PAULO, SP, BRASIL; 2.INCOR, SAO PAULO, SP, BRASIL.
Renal sympathetic nerve activity (rSNA) at low frequency influences renal handling of sodium and
water, without changing renal blood flow and glomerular filtration rate. Accordingly, we have
previously demonstrated that renal nerve stimulation increases NHE3-mediated sodium
reabsorption due to an increased intrarenal renin-angiotensin-system (RAS), independent of
hemodynamic changes. In the present study, we aimed to evaluate how physiological
sympathoexcitation accompanied by blood pressure elevation would impact renal function and
proximal tubule NHE3 regulation. To this end, we disinhibited the paraventricular nucleus of the
hypothalamus (PVN) by microinjecting the antagonist GABA-Areceptor bicuculline into the PVN in
anesthetized male Wistar rats. Bicuculline injected unilaterally within the PVN caused a rapid
increase in blood pressure compared with the baseline period (± 45%), accompanied by a great
increase in urinary volume (more than 300%). The increase in urinary volume was associated with
an enhance on NHE3 phosphorylation levels, suggesting that the diuretic effect induced by PVN
disinhibition and blood pressure elevation are, at least in part, due to a decrease in NHE3 activity.
Ongoing experiments from our laboratory are directed toward investigating the effects of
bicuculline within the PVN in other apical sodium transporters along the renal tubule and
evaluating the possible role of both systemic and intrarenal RAS in mediating such effects.
THEME 03 - NEUROPHYSIOLOGY
03.037 - ANGIOTENSIN 1-7 AFFECTS THE URINARY BLADDER CONTROL
LUCIANA CAMPI AURESCO1; EDUARDO MAZUCO CAFARCHIO1; JANAINA SENA DE SOUZA2; ITATIANA
FERREIRA RODART1; DANIEL PAULINO VENANCIO1; GISELE GIANNOCCO2; MONICA AKEMI SATO1
1.FACULDADE DE MEDICINA DO ABC, SANTO ANDRE, SP, BRASIL; 2.FEDERAL UNIVERSITY OF SAO
Aim: This study investigated the effect of i.v. angiotensin 1-7 (Ang-1-7) on intravesical pressure
(IP) and cardiovascular parameters, and also the gene expression of MAS receptors in the urinary
bladder.
Methods: Adult female Wistar rats (~250 g, N=6) were anesthetized with 2% isoflurane in 100% O2
and submitted to femoral artery and vein cannulation for mean arterial blood pressure (MAP) and
heart rate (HR) recording and also for drug administration. The urinary bladder was cannulated
for IP measurement. A miniaturized Doppler flow probe was placed around the left renal artery
for the indirect measurement of blood flow and determination of renal conductance (RC). After
baseline MAP, HR and blood flow recordings, Ang 1-7 (24 umol/kg) or saline was injected i.v.. In
another group of rats, RT-PCR was performed in the urinary bladder samples in order to determine
the gene expression of MAS receptors. Data are as mean ± SE and were subjected to paired Student
t-test. (p <0.05).
Results: The i.v. injection of Ang 1-7 reduced the IP (-20.2±1.4%) compared to saline (0.4±1%) 15
minutes after the injections, and also increased RC (38.7±6.7 % vs. 7.8±0.5% saline) without
producing significant changes in MAP and HR. The RT-PCR showed the gene expression of MAS
receptors in the urinary bladder.
Conclusion: The Ang 1-7 iv evoked renal vasodilation and reduced the IP suggesting that the Ang
1-7 likely acts directly on the urinary bladder binding to MAS receptors.
03.038 - NORADRENALINE MICROINJECTED INTO THE DORSAL PERIAQUEDUCTAL GRAY MATTER
CAUSES ANXIOLYTIC-LIKE EFFECTS IN RATS TESTED IN THE ELEVATED T-MAZE
VIVIANE BATISTA ESTRADA1; NATÁLIA KIMIE MATSUBARA1; MARCUS VINICIUS DE MATOS GOMES2;
FERNANDO MORGAN DE AGUIAR CORRÊA3; LUIZ FERNANDO VERÍSSIMO1; DENIS CARLOS DOS
SANTOS1; VINICIUS LUCCA VOLPINI1; GISLAINE GARCIA PELOSI1
1.UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL; 2.UNIVERSIDADE DO NORTE DO
PARANÁ, LONDRINA, PR, BRASIL; 3.UNIVERSIDADE DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL.
The dorsal periaqueductal gray matter (dPAG) is involved in the integration of behavioral reaction.
Panic and generalized anxiety disorder have a high prevalence throughout life. Some studies
suggest an involvement of dPAG noradrenergic system on modulating of anxiety in rats; however,
there is no evidence about its role in panic attacks. The aim of this study was to evaluate the
effect of NA microinjection into the dPAG of rats submitted to elevated T-maze (ETM), an animal
model to anxiety study.
Male Wistar rats were implanted with guide cannula aimed at receiving the dPAG. NA was
microinjected at doses of 3, 15, or 45 nmol / 50nL or artificial cerebrospinal fluid dPAG just before
being submitted to the ETM. NA on dose of 45 nmol/50nl intra-dPAG decreased inhibitory
avoidance behavior in the elevated T-maze (p<0,05) without changing escape, suggesting only
anxiolytic effect. Furthermore, the microinjection of NA did not change the general exploratory
activity of the animals submitted to the open field test, indicating that the anxiolytic-like effect
is not due to an increase in exploratory activity. The results indicate an involvement of
noradrenergic neurotransmission in the dPAG on defensive reactions associated with generalized
anxiety, but not with panic, in rats submitted to the elevated T-maze.
03.039 - ANTI-NOCICEPTIVE AND ANTI-OEDEMATOGENIC ACTIVITIES OF PIPERIC ACID
POLIANA DE ARAUJO OLIVEIRA1; RAMOM GUERRA DE OLIVEIRA2; MARIO LUIZ ARAUJO ALMEIDA
VASCONCELOS2; BRUNO GUIMARÃES MARINHO 1
1.UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, SEROPÉDICA, RJ, BRASIL; 2.UNIVERSIDADE
FEDERAL DA PARAÍBA, JOÃO PESSOA, PB, BRASIL.
The aim of this study is to assess the anti-nociceptive and anti-inflammatory activities of piperic
acid in acute pain and inflammation tests. Swiss male mice were used (20-23g - 6 animals per
group). The animals were orally administered with piperic acid at doses of 1, 5 and 10 mg/kg,
dexamethasone (2.5 mg/kg) and morphine (3 mg/kg) in the acetic acid-induced abdominal
writhing and paw oedema tests. The protocol was approved by the Ethics Committee of UFRRJ
under number 23083.005047/2011-53. Statistical significance is performed by applying an oneway or two-way analysis of variance (ANOVA), followed by the Bonferroni test. *p <0.05; ** p <0.01
and *** p <0.001 were used as level of significance. In the acetic acid-induced abdominal writhing
test were obtained the following results (mean ± SEM): number of writhes - Control – 54.2±6.1;
Vehicle – 57.5 ± 5.2; Morphine – 25.4±3.5*; 1 mg/kg – 24.7±5.7*; 5 mg/kg – 26.2±7.9*; 10 mg/kg –
12.0±4.8***. In the paw oedema test were obtained the following results (mean ± SEM): area under
curve - Control – 145.6±18.0; Vehicle – 133.0±14.0; Dexamethasone – 48.6±9.0***, 1 mg/kg –
145.0±15.0; 5 mg/kg – 66.6±9.0**; 10 mg/kg – 57.5±8.0**. Piperic acid presented effect in the
acetic acid-induced abdominal writhing and paw oedema tests, showing anti-nociceptive and antioedematogenic activities.
03.040 - MECHANISMS INVOLVED IN THE NEUROPROTECTION PROMOTED BY ANG-(1-7) IN
HIPPOCAMPAL SLICES SUBMITTED TO OXYGEN AND GLUCOSE DEPRIVATION
ANDRÉ LUIZ DE OLIVEIRA; GABRIELA MARIA DE ABREU GOMES; ROSÁRIA DIAS AIRES; TALITA HELEN
FERREIRA-VIEIRA; ROBSON AUGUSTO SOUZA DOS SANTOS; ANDRÉ RICARDO MASSENSINI
Recent reports has shown that the ACE2/Ang-(1-7)/Mas receptor pathway plays a protective role
in brain ischemic damage, but the mechanisms are not completely elucidated.
In the present work, we investigate which mechanism would be involved in the neuroprotection
promoted by Ang-(1-7) in hippocampal slices submitted to oxygen and glucose deprivation
To address this question, 400 µM hippocampal slices from Swiss male mice submitted to oxygen
and glucose deprivation (OGD) were used to evaluate cell death, Nitric Oxide (NO2) release and
molecules involved in apoptosis signaling (Caspase 3, 8 and 9). Test Groups were treated with
Ang-(1-7); Ang-(1-7)+A-779 and A-779.
Ang-(1-7) showed a neuroprotective effect, since cell death in hippocampal slices was reduced
after OGD in presence of Ang-(1-7) (5.8 ± 0.9 vs 18.7 ± 4.3; p<0.05). After OGD and 4h of
reperfusion, Ang-(1-7) reduced both Caspase 3 (2916.5 ± 357.9 vs 4714.9 ± 344.1) and Caspase 9
(112.5 ± 5.2 vs 104.3 ± 4.0) activity to control levels. These effects were abolished by A-779
(2916.5 ± 357.9 vs 5246.4 ± 634.8 and 112.5 ± 5.2 vs 147.5 ± 4.8, respectively). Caspase 8 activity
was not altered in the presence of Ang-(1-7). Nitric oxide levels decreased in the presence of Ang(1-7) during OGD (0.286 ± 0.02 vs 0.516 ± 0.06) and after reperfusion (0.393 ± 0.05 vs 0.657 ±
0.09). These effects were abolished by A-779 during OGD (0.286 ± 0.02 vs 0.519 ± 0.04) and after
reperfusion (0.393 ± 0.05 vs 0.741 ± 0.06).
In conclusion, Ang-(1-7) acts reducing NO2 levels and both Caspase 9 and 3 activity.
03.041 - ANTI-NOCICEPTIVE EFFECT OF OURATEA HEXASPERMA AND OURATEA FERRUGINEA
LILIAN UCHOA CARNEIRO1; QUELI CRISTINA FIDELIS2; MÁRIO GERALDO DE CARVALHO2; BRUNO
GUIMARÃES MARINHO1
1.FEDERAL RURAL UNIVERSITY OF RIO DE JANEIRO, SEROPEDICA, RJ, BRASIL; 2.FEDERAL
UNIVERSITY OF PARAIBA, JOÃO PESSOA, PB, BRASIL.
The aim of this study was to assess the anti-nociceptive activity of Ouratea hexasperma (OH) and
Ouratea ferruginea (OF) in acute pain tests. Swiss male mice were used (20-23g - 6 animals per
group). The animals were orally administered with OH and OF at dose of 10 mg/kg, acetylsalicylic
acid (ASA, 200 mg/kg) and morphine (M, 3 mg/kg) in the acetic acid-induced abdominal writhing
34
and formalin tests. The protocol was approved by the Ethics Committee of UFRRJ under number
24088.005435/2011-58. Statistical significance was performed by applying an one-way analysis of
variance (ANOVA), followed by the Bonferroni test. *p <0.05; ** p <0.01 and *** p <0.001 were used
as level of significance. In the acetic acid-induced abdominal writhing test were obtained the
following results (mean+SEM): number of writhes - Control–54.2+6.1; Vehicle–57.5+5.2; M–
25.4+3.5***; OH 10 mg/kg–9.7+1.2***; OF 10 mg/kg–6.5+2.0***. In the formalin test were obtained
the following results (mean + SEM): Time of licking (s) – First phase: Control–53.2+6.3; Vehicle–
50.0+3.6; M–33.5+4.6*, ASA–40.0+4.0; OH 10 mg/kg–28.4+7.2*; OF 10 mg/kg–34.6+5.0; Second
phase: Control–175.2+15.0; Vehicle–173.0+7.8; M–84.8+4.2***, ASA–79.0+8.0***; OH 10 mg/kg–
77.7+9.0***; OF 10 mg/kg–103.6+14.3***. OH and OF presented effect in the acetic acid-induced
abdominal writhing test. OH presented effect in both phases in the formalin test, indicating
central anti-nociceptive activity and OF presented effect only in second phase, indicating
peripheral anti-nociceptive activity.
03.042 - ACE2-ANG-(1-7)-MAS AXIS AND MODULATION OF CALCIUM SIGNALING IN BRAIN
ISCHEMIA VIA AMPAR INTERNALIZATION
GABRIELA MARIA DE ABREU GOMES; ANDRÉ LUIZ DE OLIVEIRA; ROBSON AUGUSTO SOUZA DOS
SANTOS; ANDRÉ RICARDO MASSENSINI
Angiotensin-(1-7) [Ang-(1-7)] is a key component of the renin–angiotensin system (RAS), been
primarily produced from Angiotensin II by angiotensin-converting enzyme 2 (ACE2). Ang-(1-7)
exerts vasodilatory and antioxidative effects via its G protein-coupled receptor Mas, which is
widely expressed in the brain.
The aim of the present study was to investigate the mechanism of Ang-(1-7) induced
cerebroprotection, as understanding these processes would further support the rationale for
activating the ACE2/Ang-(1-7)/Mas axis as a potential stroke therapy.
To address this question, 400 µM hippocampal slices from Swiss male mice, FVB/N mice and Mas
Knockout (MasKO) mice submitted to oxygen and glucose deprivation (OGD) were used to evaluate
cell death.
Ang-(1-7) protected the hippocampal slices in different concentrations, Ang-(1-7) 100nM (49,8 ±
8,7 vs 75,8 ± 11,3); Ang-(1-7)500nM (33,05 ± 3,017802 vs 75,8 ± 11,3); Ang-(1-7)1µM (29,01 ± 1,8
vs 75,8 ± 11,3); Ang-(1-7)10µM (21,11 ± 1,88 vs 75,8 ± 11,3); Ang-(1-7)100µM (32,23 ± 5,79 vs 75,8
± 11,3). MasKO hippocampal slices, curiously, did not show a great ceall death, instead it was
protected against ischemia, when compared with Wild Type mice (10,87 ± 2,37 vs 25,97 ± 5,79).
In conclusion, Ang-(1-7) can protect cerebral slices from in vitro ischemia, with the best
concentration at 10µM. On the other hand, Ang-(1-7) seems act by activating another pathway,
instead only through the Mas receptor.
03.043 - EXPOSURE TO BISPHENOL A MODULATES NEUROGLOBIN AND CYTOGLOBIN IN THE
BRAIN
RODRIGO RODRIGUES DA CONCEICAO1; JANAINA SENA DE SOUZA1; KELEN CARNEIRO OLIVEIRA1;
MARINA MALTA LETRO KIZYS1; RENATA MARINO ROMANO2; MARCO AURELIO ROMANO2; MAGNUS
RÉGIOS DIAS DA SILVA1; MARIA IZABEL CHIAMOLERA1; GISELE GIANNOCCO1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE ESTADUAL DO
CENTRO-OESTE, GUARAPUAVA, PR, BRASIL.
Bisphenol A (BPA) is a chemical compound synthesized by condensation of two phenol groups and
an acetone molecule, is used as a monomer in the production of polycarbonate plastic.
One of the characteristics of BPA is the ability to cross the blood - brain barrier, furthermore, Kim
et al 2004 showed that brain tissue BPA accumulates 18-41% more than in plasma. And this could
explain the interference of BPA in the development of neurons in areas such as the cerebellum,
cortex and hippocampus.
The aim of the present study is to analyze BPA action on gene expression of Neuroglobin ( (NGB)
and Cytoglobin (CYGB) in different regions of the brain. In this study, prepubertal male Wistar
rats were orally treated with 5 and 25 mg/kg/day BPA from postnatal day 23 (PND23) to PND58
and sacrificed at PND102. Total RNA was extracted from Cerebellum, Cortex, Hypothalamus. In
cerebellum and cortex found increases in gene expression NGB and CYGB remained unchanged,
however the hippocampus found reduced NGB and CYGB gene expression
In Sumary, The BPA-exposed animal module modulates gene expression of NGB and CYGBin the
brain of anatomically specific way.
03.044 - EFFECT OF EXPERIMENTAL GESTATIONAL HYPOTHYROIDISM ON NOCICEPTIVE
RESPONSE AFTER MORPHINE APPLICATION
IURA GONZALEZ NOGUEIRA-ALVES; MÁRCIO COUTO GOMES; VANESSA CIBELLE BARBOZA DE
CARVALHO; ANTONIO LUCAS SILVA-FILHO; HELAINE CRISTINA BATISTA DOS SANTOS; DEMETRIUS
SILVA DE SANTANA; JULIO CÉSAR SANTANA ALVES; VALTER JOVINIANO SANTANA-FILHO; DANIEL
BADAUÊ-PASSOS, JR.
UNIVERSIDADE FEDERAL DE SERGIPE, SÃO CRISTÓVÃO, SE, BRASIL.
A lack in maternal thyroid hormones levels during gestation may affect offspring development. In
present work we aimed to investigate the effects of experimental gestational hypothyroidism
(EGH) on supraspinal nociceptive response and on opioidergic system of offspring. EGH was
induced by methimazole (MMI) 0.02% in drinking water from day 9 of gestation until birth. Male
offspring was weighed and supraspinal nociception was assessed, at 60 PND, by using Hot Plate
(Insight, Brazil) at 52 ± 0.2 °C. The latency to rearing paw lick reflex was measured. Basal latency
(time 0) was measured. At 30, 60, 90 and 120 min after morphine injection (3 mg/kg i.p.),
repeated measurements of latency were performed. No difference was observed in body weight
in offspring from methimazole treated dams (OMTD, n = 9) when compared to control (offspring
from water treated dams, OWTD; n = 13). A significant interaction in time and treatment factors
was observed (F(4, 80) = 2,50; p < 0,05). It may be confirmed by a greater latencies in OMTD at
times 30 (10.41± 0.9 vs 12.75 ± 1.0 s) and 60 min (11.60±0.7 vs 13.29± 0.3 s), when compared to
OWTD, whereas smaller latencies were shown at 90 (11.19 ± 1.0 vs 10.37±0.8 s) and 120 minutes
(9.79±1.0 vs 8,38±0.65 s). We conclude that EGH modifies thermal nociception in the offspring
but only when opioidergic system is recruited.
03.045 - EFEITO ANSIOLÍTICO DO ENRIQUECIMENTO AMBIENTAL EM CAMUNDONGOS MACHOS
JOVENS
RAPHAEL DA SILVA LAU; FÁBIO FAGUNDES DA ROCHA; CLAUDIO DA SILVA ALMEIDA; ROBERTO
LAUREANO-MELO; CAMILA MENDONÇA NETTO JOBIM
UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, SEROPÉDICA, RJ, BRASIL.
Objetivo
Trabalhos anteriores de nosso grupos mostraram o efeito ansiolítico perinatal e pós-desmame e,
camundongos adultos submetidos ao enriquecimento ambiental (EA), o objetivo deste trabalho foi
verificar se este efeito ansiolítico também ocorre em camundongos jovens (28 dias).
Métodos
Camundongos Swiss foram acasalados em duas condições: EA (caixa maior, com plataforma
elevada, esconderijos e material para aninhamento) ou padrão (P). Após o nascimento dos
filhotes, os mesmos foram mantidos nos ambientes que os pais viviam (enriquecido ou padrão) até
a análise comportamental que ocorreu aos 28 dias de vida e consistiu da seguinte bateria de
testes: labirinto em cruz elevado (LCE), caixa claro-escuro (CCE) e campo aberto com intervalo
de 2 dias entre eles. Neste experimento foram utilizados apenas os filhotes machos. Os resultados
foram analisados através teste T-student, sendo considerados significativos valores de P<0,05.
Resultados
No teste do LCE houve aumento significativo na porcentagem de tempo de permanência nos braços
abertos dos animais do grupo E (37,5 ± 4,6 %) em comparação do grupo C (25,8 ± 2,8 %). No teste
da CCE, verificou-se uma redução significativa da latência de transição do ambiente escuro para
o claro no grupo E (5,1 ± 0,9 s) em relação ao grupo C (7,7 ± 1,4 s). Outros parâmetros destes
testes assim como do teste do campo aberto não foram alterados significativamente.
Conclusão
Estes dados mostram que os camundongos jovens expostos EA reduziram comportamentos
análogos à ansiedade indicando que este efeito pode ser observado mesmo em idade precoce.
03.046 - NEW SENSORS AND MONITORS FOR PHYSIOPATOLOGICAL ACADEMIC RESEARCH AND
INTENSIVE CARE UNITS
GUSTAVO HENRIQUE FRIGIERI VILELA1; SÉRGIO MASCARENHAS1; RODRIGO DE ALBUQUERQUE
PACHECO ANDRADE1; FRANCISCO TALLARICO1; DEUSDEDIT LINEU SPAVIERI JUNIOR1; RODRIGO
BRUNELLI2; CHARLES CHENWEI WANG1; LÍGIA ALARCÃO GOMIERO1; RAFAEL LUIS CAPORAL3;
JANAINA MASCARENHAS HORNOS COSTA1; PAULO ROBERTO MASCARENHAS1; YVONE MARIA
MASCARENHAS1
1.BRAINCARE HEALTH TECNOLOGY, SÃO CARLOS, SP, BRASIL; 2.INSTITUTO DE FÍSICA DE SÃO
CARLOS - UNIVERSIDADE DE SÃO PAULO, SÃO CARLOS, SP, BRASIL; 3.DEPARTAMENTO DE
COMPUTAÇÃO - UNIVERSIDADE FEDERAL DE SÃO CARLOS, SÃO CARLOS, SP, BRASIL.
Braincare is a startup company that was born with the support of Brazilian funding agencies, with
the goal of providing innovative tools for researchers and health professionals for scientific
development and multimodal monitoring of medical parameters. The main innovations brought by
Braincare company are a new intracranial pressure (ICP) monitoring systens, using minimally
invasive and non invasive sensors. The results obtained from experiments on animals and humans
indicate the ability to monitor ICP without the need to invade the intracranial space, currently
used in hospitals. The advent of such sensors makes it possible to open new areas of research,
since it allows the ICP study in animals and humans. In addition to intracranial pressure, Braincare
monitors also works with sensors for the monitoring of invasive and noninvasive blood pressure,
temperature, EKG, SPO2 and has the ability to read the invasive ICP sensors. All monitoring is
performed with the recording of the data at high acquisition rates, enabling excellent statistics
analysis of the stored data. The devices also have there own software, developed by Braincare
that bring new parameters analysis of cerebral parameter, such as compliance, PRx and RAP. This
paper presents results obtained with these new methods opening new possibilities in neuroscience
and neurosurgery research.
03.047 - DIGITAL AUTOMATED MATHEMATICAL TOOLS TO ACQUIRE, STORE AND ANALYSE
PHYSIOPATHOLOGICAL PARAMETERS
GUSTAVO HENRIQUE FRIGIERI VILELA1; DEUSDEDIT LINEU SPAVIERI JUNIOR1; RODRIGO DE
ALBUQUERQUE PACHECO ANDRADE1; RODRIGO BRUNELLI2; FRANCISCO TALLARICO1; RAFAEL LUIS
CAPORAL3; CHARLES CHENWEI WANG1; LÍGIA ALARCÃO GOMIERO1; JANAINA MASCARENHAS HORNOS
COSTA1; YVONE MARIA MASCARENHAS1; PAULO ROBERTO MASCARENHAS 1; SÉRGIO MASCARENHAS1
1.BRAINCARE HEALTH TECNOLOGY, SÃO CARLOS, SP, BRASIL; 2.INSTITUTO DE FÍSICA DE SÃO
CARLOS - UNIVERSIDADE DE SÃO PAULO, SÃO CARLOS, SP, BRASIL; 3.DEPARTAMENTO DE
COMPUTAÇÃO - UNIVERSIDADE FEDERAL DE SÃO CARLOS, SÃO CARLOS, SP, BRASIL.
Analysis of data obtained with experiments on animals and humans still brings a series of
challenges to research groups, these data are results of the interaction of different biological
systems, which makes them mostly nonlinear. Highly sophisticated mathematical and statistical
signal analysis is in general needed. This is the case for results obtained with the noninvasive
intracranial pressure recently introduced by our group. For these propose we offer data analysis
center with necessary mathematical and statistical tools for signal analyses. Our methods allows
obtaining variety of quantitative parameters such as linear and non-linear data analyses. The
system uses several algorithms that allows acquisition, store and analysis in a standardized
protocol. The users uploads the data to be analyzed to an Internet server, select the analysis that
must be performed and the system does all the mathematical work, returning to the user's e-mail
the results in tables and graphs. The online system also hosts a database, that allows to the
coordinators the access to all results. The objective of this work is present the results obtained
with these tools.
03.048 - ANTIDEPRESSANT BEHAVIOR IN THYROIDECTOMIZED WISTAR RATS IS INDUCED BY
HIPPOCAMPAL HYPOTHYROIDISM
RODRIGO RODRIGUES DA CONCEICAO1; ROBERTO LAUREANO-MELO2; JANAINA SENA DE SOUZA1;
KELEN CARNEIRO OLIVEIRA1; CLAUDIO DA SILVA ALMEIDA3; MARIA CLARA CARVALHO MELO1; GISELE
GIANNOCCO1
1.UNIFESP, SAO PAULO, SP, BRASIL; 2.UFRRJ, SEROPEDICA, RJ, BRASIL; 3.UFFRJ, SEROPEDICA, RJ,
BRASIL.
The aim of our study was to verify how thyroidectomy influences on behavioral parameters and
its relation to hippocampus´ thyroid hormones metabolism and neurogenesis. For this purpose,
adult male Wistar rats underwent to thyroidectomy to induce hypothyroidism. Behavioral tests,
the thyroid profile and hippocampal genic expression were evaluated. It was observed that
thyroidectomized group had a significantly increased in serum TSH (945%, p < 0.0001) and a
decreased in T4 levels (395%, p < 0.0001). However, there were no difference in serum levels of
T3. It was also observed a significant reduction of the MCT-8 (400%, p = 0.0008), TRα1 (800%, p <
0.0001), DIO2 (800%, p = 0.0004), ENPP2 (266%, p = 0.02) and BDNF (350%, p = 0.002) mRNA
expression in hippocampus of thyroidectomized animals. In the forced swimming test, it was
verified that thyroidectomy promotes a decrease in time of immobility (152%, p < 0.0001) and
climbing (219%, p < 0.0001) when compared with the control group. In summary, we conjecture
that the antidepressant behavior observed in thyroidectomized Wistar rats could be induced by
hippocampal hypothyroidism.
03.049 - BEHAVIORAL RESPONSES EVOKED BY DIFFERENT EXPERIMENTAL PROTOCOLS
CHALLENGING SODIUM BALANCE: PROTOCOL STANDARDIZATION
LÍVIA DA ROCHA NATALINO MONTEIRO1; IRACEMA GOMES DE ARAUJO1; LUIS CARLOS REIS1; CHARLES
COLIN HINDMARCH2; ANDRÉ DE SOUZA MECAWI1
1.UNIVERSIDADE FEDERAL RURAL DO RIO DE JANEIRO, SEROPÉDICA, RJ, BRASIL; 2.FACULTY OF
MEDICINE, UNIVERSITY OF MALAYA, KUALA LUMPUR, MALASIA.
The maintenance of sodium and water balance is crucial for all biological functions. Mammals
have developed complex systems in order to maintain hydromineral balance that integrate renal,
neuroendocrine and behavioral responses to avoid or correct significant changes at water and
sodium concentrations. The present study aimed to determine the functional profile of the
responses evoked by different experimental protocols challenging sodium balance. Wistar male
rats were randomly divided into 4 groups: (i) control; (ii) low-sodium diet; (iii) low-sodium diet
associated furosemide in drinking water (20mg/kg); (iv) saline overload (0.3 M NaCl instead of
water). Animals were housed into individual metabolic cages where food, fluid and urinary
excretion were measured during 5 days. There were significant differences in daily water intake
between groups (ii) and (iii) in the last day of evaluation (26.2 ± 4.1 vs 9.3 ± 7.9 mL/100g bw).
Hypertonic saline (0.3 M NaCl) consumption was higher since the second day in group (iv) when
compared with control (22.4 ± 2.6 vs 4.3 ± 0.9 mL/100g bw). Urinary volume was also higher in
group (iv) since the second day of evaluation (17.6 ± 2.5 vs 6.0 ± 0.9 mL/100g bw) as well as in
group (iii) when compared with low-sodium diet group (20.4 ± 3.6 vs 6.2 ± 0.9 mL/100g bw). These
results reinforce the role of thirst and sodium appetite as important mechanisms evoked to correct
water and sodium imbalance.
03.050 - HOME-BASE FORMATION DEPENDS OF MATERNAL THYROID HORMONES DURING
INTRAUTERINE LIFE IN RATS
EDÊNIA DA CUNHA MENEZES1; AMANDA GONTIJO-MOREIRA1; MAURÍCIO SILVA SANTOS1; TIMNA DE
JESUS SANTOS1; LOIC HERNANDEZ DO AMARAL ARAGÃO1; VANESSA CIBELLE BARBOZA DE
CARVALHO1; PATRÍCIA RABELO DOS SANTOS1; IURA GONZALEZ NOGUEIRA-ALVES1; CASSIO MORAIS
LOSS2; DIOGO LOSCH DE OLIVEIRA2; GIORDANO GUBERT VIOLA1; DANIEL BADAUÊ-PASSOS, JR.1
1.UNIVERSIDADE FEDERAL DE SERGIPE, SÃO CRISTÓVÃO, SE, BRASIL; 2.UNIVERSIDADE FEDERAL DO
RIO GRANDE DO SUL, PORTO ALEGRE, RS, BRASIL.
Inadequate supply of maternal thyroid hormones to fetus has been shown to be critical for the
imprinting of abnormal behavior after birth. We investigated the effect of experimental
gestational hypothyroidism (EGH) on exploratory and locomotor behaviors and on home-base
formation in offspring on postnatal days (PND) 75 and 120. EGH was induced by the administration
of 0.02% methimazole (MMI) in drinking water from the 9th day of gestation until birth. Male
offspring from water treated dams (m-OWTD) or from methimazole treated dams (m-OMTD) were
placed in open field exploratory task on both ages. Total distance traveled in the whole arena was
evaluated during the whole 15 min of experimental observation, or in 3 consecutive 5-minute
intervals (0-5, 5-10, and 10-15 min). Percent of time spent at the home base was also evaluated.
35
ANY-maze software was used for tracking behavior. Student t test and 2-way ANOVA were used
when appropriate. No difference was observed in the total distance and period distance traveled
between groups in both age points (p > 0.05). However, time spent at the home base was lower
in m-OMTD on PND 75 when compared to control (38.77 ± 5,205 versus 24.74 ± 3,493 %, p<0,05,
n=13). In conclusion, we suggested that EGH induces changes in the spatial organization of
locomotor behavior in rats, in despite of unaltered total distance traveled in whole arena.
03.051 - CAFFEINE TREATMENT DURING PREGNANCY AND LACTATION ALTERS BEHAVIORAL
PROFILE OF MICE OFFSPRING
ROBERTO LAUREANO-MELO1; JOSIE MARCELLE LIRA ALBUQUERQUE 2; ANDERSON LUIZ BEZERRA DA
SILVEIRA1; FENANDO AZEVEDO CRUZ SEARA1; CLAUDIO DA SILVA ALMEIDA1; WELLINGTON SILVA
CORTES1
1.UFRRJ, NILOPOLIS, RJ, BRASIL; 2.UFF, NITEROI, RJ, BRASIL.
The objective was to assess the behavioral profile in offspring of Swiss mice treated during
pregnancy and lactation with caffeine. For this purpose, two groups (n=6 each, and BW~35g) of
female mice were treated during pregnancy and lactation by: tap water and caffeine solution at
a concentration of 0,3mg/mL through oral route. The offspring obtained, by completing 70 days
of life, was underwent a behavioral battery test. Statistical analysis was performed by student t
test and the different significance adopted was p<0.05. It was not found any significant
differences in the in light-dark box, elevated plus maze, tail suspension and forced swimming
tests. In anxiety related responses in the open field test, however, the mice of caffeine group had
more entries in center zone (31%, p=0.03) and greater distance traveled in center zone (36%,
p=0.04). Moreover, in the marble burrying test, there was a significant decrease in the number of
buried marbles compared with controls (110%, p=0,002). In the meantime, in the von Frey test, it
was observed an exacerbation of mechanical allodynia both in basal conditions and after the
carrageenin administration (p < 0.0001). Furthermore, we hypothesize that caffeine treatment
during pregnancy and lactation results in long-term in epigenetic changes in the expression,
binding and density of brain adenosine receptors. Consequently, these possible alterations may
have led a cytoarquitetonic alterations in limbic and in spinal sites associated with anxiety-like
behavior and modulation of nociceptive responses, respectively.
03.052 - DIETA HIPOSSÓDICA POR QUATRO DIAS LEVA A RESPOSTAS ANSIOLÍTICAS NO MODELO
LABIRINTO EM CRUZ ELEVADO
EVANDRO VALENTIM DE LIMA1; TATIANE VILHENA FRANCO2; QUÉZIA SANCHES RODRIGUES
CARBALAN1; IRACEMA GOMES DE ARAUJO1; LUIS CARLOS REIS1; LUCILA LEICO KAGOHARA ELIAS2;
JOSÉ ANTUNES-RODRIGUES2; ANDRÉ DE SOUZA MECAWI1
1.DEPARTAMENTO DE CIÊNCIAS FISIOLÓGICAS, INSTITUTO DE BIOLOGIA, UFRRJ, SEROPÉDICA, RJ,
BRASIL; 2.DEPARTAMENTO DE FISIOLOGIA, FACULDADE DE MEDICINA DE RIBEIRÃO PRETO,
UNIVERSIDADE DE SÃO PAULO, RIBEIRÃO PRETO, SP, BRASIL.
O apetite ao sódio é resposta essencial para a manutenção osmolaridade e o volume de líquido
extracelular. Esse estudo visa avaliar as alterações da locomoção e ansiedade no modelo do
labirinto em cruz elevado após quatro dias de dieta hipossódica.
Ratos Wistar (60 dias) foram submetidos à dieta normossódica (CTRL, 1% NaCl) ou hipossódica
(HIPO - NaCl < 0,1) por 4 dias. Ao final desses, foram submetidos ao teste do labirinto em cruz
elevado por 5 minutos.
Foi observada uma resposta ansiolítica e aumento da locomoção do grupo HIPO. Houve aumento
do número de entradas nos braços abertos (p < 0,01) e fechados (p < 0,01) e do número de
quadrantes cruzados nos braços abertos (p < 0,05) e fechados (p < 0,01). Sendo, o número de
quadrantes cruzados nos braços abertos maior do que nos fechados (p < 0,01). Observou-se ainda
o aumento no número de vezes em que HIPO frequentaram a extremidade dos braços abertos (p
< 0,01), com aumento da relação frequência de idas no ultimo quadrante dos braços abertos/
fechados (p < 0,01). Também houve diminuição do tempo de permanência dos animais nos braços
fechados (p < 0,05) e aumento nos abertos (p < 0,01), com aumento da relação tempo de
permanência nos braços abertos/braços fechados (p < 0,05). Foram observados aumento nos
mergulhos com a cabeça nos braços abertos (p < 0,001) e exploração vertical (p < 0,001). Assim,
nosso estudo indica que a dieta hipossódica foi capaz de aumentar o comportamento exploratório
e a resposta ansiolítica em ratos.
03.053 - HISTAMINE IN THE BASOLATERAL AMYGDALA PROMOTES INHIBITORY AVOIDANCE
LEARNING INDEPENDENTLY OF HIPPOCAMPUS
FERNANDO BENETTI1; CRISTIANE REGINA GUERINO FURINI2; JOCIANE DE CARVALHO MYSKIW2;
MARIA BEATRICE PASSANI3; LEONARDO MUNARI4; GUSTAVO PROVENSI 3; ELISABETA BALDI3;
CORRADO BUCHERELLI3; IVAN IZQUIERDO2; PATRIZIO BLANDINA3
1.UFRGS, PORTO ALEGRE, RS, BRASIL; 2.PUCRS, PORTO ALEGRE, RS, BRASIL; 3.DIPARTIMENTO DI
NEUROSCIENZE, PSICOLOGIA, AREA DEL FARMACO E SALUTE DEL BAMBINO, SEZIONE DI
FARMACOLOGIA E TOSSICOLOGIA, UNIVERSITÁ DI FIRENZE, 50139 FIRENZE; DIPARTIMENTO DI
MEDICINA SPERIMENTALE E CLINICA, UNIVERSITÁ DI FIRENZE, 50134 FIRENZE, ITALY, ITALIA;
4.DEPARTMENT OF PHARMACOLOGY AND SYSTEMS THERAPEUTICS, MOUNT SINAI SCHOOL OF
MEDICINE, NEW YORK, ESTADOS UNIDOS.
Recent discoveries demonstrated that recruitment of alternative brain circuits permits
compensation of memory impairments following damage to brain regions specialized in integrating
and/or storing specific memories, including both dorsal hippocampus and basolateral amygdala
(BLA). Here, we first report that the integrity of the brain histaminergic system is necessary for
long-term memory (LTM), but not for short-term memory (STM) of step-down inhibitory avoidance.
Second, we found that phosphorylation of cAMP responsive-element-binding protein, a crucial
mediator in LTM formation, correlated anatomically and temporally with histamine-induced
memory retrieval in different phases of memory consolidation. Third, we found that exogenous
application of histamine in either hippocampal CA1 or BLA of brain histamine-depleted rats, hence
amnesic, restored LTM; however, the time frame of memory rescue was different for the two
brain structures, immediately posttraining for BLA, long lasting (up to 6 h) for the CA1. Moreover
LTM was formed immediately after training restoring of histamine transmission only in the BLA.
These findings reveal the essential role of histaminergic neurotransmission to provide the brain
with the plasticity necessary to ensure memorization of emotionally salient events, through
recruitment of alternative circuits. Hence, our findings indicate that the histaminergic system
comprises parallel, coordinated pathways that provide compensatory plasticity when one brain
structure is compromised openning new possibilities for targeted treatment of memory disorders.
03.054 - INTERMITTENT SUCROSE ACCESS AFFECTS SENSITIZED NEED-FREE SODIUM INTAKE IN
RATS
BRUNA MAITAN MAITAN SANTOS; RICHARD BOARATO DAVID; CARINA A.F. ANDRADE; JOSÉ
VANDERLEI MENANI; LAURIVAL ANTONIO DE LUCA JR
FACULDADE DE ODONTOLOGIA DE ARARAQUARA - UNESP, SANTA CRUZ DO RIO PARDO, SP, BRASIL.
Preliminary results show history of sodium depletion increasing sucrose intake in sucroseinexperienced animals. In the present work we measured sensitized daily need-free sodium intake
in rats with restricted daily access to sucrose. Male Holtzman rats (n = 14) were submitted to 3
episodes of sodium depletion (F/C treatment): subcutaneous injection of diuretic furosemide (10
mg/kg) combined with angiotensin converting enzyme inhibitor captopril (5 mg/kg) or control
treatment (vehicle + saline) given every other 2 days. Ten days after the last F/C or control
treatment, sucrose intake (only 10% sucrose available 2 h/day) was evaluated for 5 consecutive
days. Daily water and 0.3 M NaCl intake were measured before (basal period) and after history of
F/C (post-F/C), and also during the days of sucrose tests (sucrose period). Both need-induced and
need-free sodium intakes were sensitized in F/C group (1st vs. 3rd F/C: 2.7 ± 0.7 ml vs. 6.1 ± 1.0
ml/2h; basal vs. post-F/C period: 6.8 ± 1.1 ml vs. 19.4 ± 2.4 ml/day, respectively). Need-free
sodium intake was attenuated comparing post-F/C versus sucrose period (19.4 ± 2.4 ml vs. 13.5 ±
2.5 ml, respectively), showing that intermittent sucrose access can alter need-free sodium intake.
Present results suggest that sugar intake can affect the enhanced sodium intake, resembling the
phenomenon of anticipatory contrast.
THEME 04 - CARDIOVASCULAR PHYSIOLOGY
04.073 - EFFECT OF SODIUM NITROPRUSSIDE (SNP) ON VENOUS BLOD FLOW IN WISTAR RATS
DANIEL PAULINO VENÂNCIO; MONICA AKEMI SATO; ALEXANDRE CESAR FIORETTI; CRISTIANA AKEMI
OGIHARA; ROBERTO LOPES DE ALMEIDA
FACULDADE DE MEDICINA DO ABC, SANTO ANDRE, SP, BRASIL.
Aim: This study investigated the effect of SNP on visceral and muscular venous blood flow (BF) in
Wistar rats.
Methods: Adult male Wistar rats (~350 g, N=6) were submitted to the cannulation of the right
femoral artery and vein for mean arterial pressure (MAP), heart rate (HR) and infusion of drugs
under 2% isoflurane in 100% O2. Doppler flow probes were placed around the left renal and left
femoral artery and vein for indirect BF recordings in a Doppler flowmeter. Vascular conductance
was calculated as the ratio of Doppler shift and mean arterial pressure (MAP). Afterwards,
isoflurane was discontinued and rats were anesthetized with i.v. α-chloralose (60 mg/kg). After a
baseline MAP, HR and BF recordings in a data acquisition system (Power Lab 16 SP), SNP 30 ug/kg
was injected i.v. before and after i.v. phentolamine (PHEN, 1mg/kg, an antagonist of α1 and α2adrenoceptors). Data are as mean±SE and were submitted to paired Student t-test (p<0.05).
Results: The injection of SNP evoked hypotension (-50±5 mmHg) and reflex tachycardia (33±7
bpm), with no changes in renal artery and vein conductance. Nevertheless, SNP increased femoral
artery (113±28%) and vein (1036±670%) conductance. After PHEN, the injection of SNP still elicited
an increase in femoral artery conductance (109±21%), but the increase in femoral vein
conductance was abolished (131±123%).
Conclusion: The SNP can induce femoral venodilation through a mechanism which is dependent
on a downstream signaling crosstalk with α-adrenoceptors pathway.
04.074 - INFLUENCE OF THE OVARIAN HORMONES ON THE CORONARY EFFECTS OF
ANGIOTENSIN-(1-7)
JULIANNA ALEXANDRE BATISTA SILVA; JULIANA ESTEVÃO PORTO; ALLANCER DIVINO CARVALHO
NUNES; DIEGO BASILE COLUGNATI; ELIZABETH PEREIRA MENDES; CARLOS HENRIQUE DE CASTRO
We have previously demonstrated that Angiotensin (Ang)-(1-7)-induced coronary vasodilation was
blunted in ovariectomized Wistar rats.However, it is unknown whether ovarian hormones
replacement can restore the vasodilator response to Ang-(1-7) in the coronary bed. Thus, the aim
of this study was is to evaluate the effect of the estrogen e progesterone replacement in the
coronary effects of Ang-(1-7). Female ovariectomized wistar rats (8 weeks) were treated with
progesterone or estrogen (1 mg/kg/day). At the end of the treatment, the hearts were isolated
and perfused according the Langerdorff method. After stabilization, the hearts were perfused
with Ang-(1-7) (20 pmol/L) in absence or presence of A-779 (2 nmol/L) or Losartan (1 µmol/L). As
previously demonstrated, the Ang-(1-7) induced coronary vasodilation in proestrus, but not in
diestrus. Moreover, the Ang-(1-7) induced. Moreover, the Ang-(1-7) induced a coronary
vasoconstriction in the ovariectomized rat hearts. This effect was abolished by chronic treatment
with estrogen, but not with progesterone. In addition, the vasoconstriction was also inhibited by
pre-treatment of the isolated hearts with A-779 or Losartan. These results suggest an important
role of the estrogen in the coronary effects of the Ang-(1-7).
04.075 - FIBROSIS IN ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IS NOT RELATED TO
CARDIAC DYSFUNCION
PATRICIA FIDELIS DE OLIVEIRA GREGOLINI1; LUIZ BORGES BISPO-DA-SILVA2; VICTOR HUGO MARTINS
DE MIRANDA1; GRASIELLE DE OLIVEIRA LOPES2; MARIANA SPINARDI1; MELIZA GOI ROSCANI3;
SAMANTA APARECIDA CASTRO 1; EDRIELI DE CÁSSIA CARLOS1; JULIANA IRANI FRATUCCI DE GOBBI1
1.UNIVERSIDADE ESTADUAL PAULISTA-UNESP, BOTUCATU, SP, BRASIL; 2.UNIVERSIDADE FEDERAL
DE UBERLÂNDIA, UBERLÂNDIA, MG, BRASIL; 3.UNIVERSIDADE FEDERAL DE SÃO CARLOS, SÃO
CARLOS, SP, BRASIL.
The isoproterenol (ISO)-induced myocardial infarction (MI) is one of the most studied experimental
models of this disease; however, the time course relationship between the morphological and
functional cardiac alterations induced by ISO still to be determined. Thus, we characterized the
early and late cardiac function in ISO-induced MI in relation to the cardiac fibrosis. MI was induced
by ISO (85mg/kg, sc) in male Wistar rats (250-300g; n=10) for 2 consecutive days. Early and late
MI (3 and 28 days after ISO, respectively) were characterized by ECHO, left ventricular
catheterization and percentage of fibrosis. Early ISO promoted cardiac dysfunction evidenced by
decreased cardiac output (CO: 70±6 vs 87±4 mL/min), increased E/A ratio (2.85±0.32 vs
1,56±0.11), increased left ventricular end-diastolic pressure (LVEDP: 10±2 vs 4±1 mmHg), reduced
contractility (DPDT+: 4755±333 vs 6885±402 mmHg/s) and cardiac relaxation (DPDT-: -2785±275
vs -4430±277 mmHg/s) when compared to controls (0.9% NaCl; n=8). Late functional assessments
showed no impairment of cardiac function when compared to controls (n=8-10; CO: 84±8 vs 85±10
mL/min; E/A ratio: 1.77±0.14 vs 2.12±0.20; LVEDP: 9±2 vs 4±1 mmHg; DPDT+: 5767±493 vs
7160±547 mmHg/s; DPDT-: -3788±313 vs -4225±521 mmHg/s in controls). However, fibrosis was
even higher at late (6.3±1.2 vs 2.7±0.3% in controls) than early MI (3.9±0.8 vs 2.1±0.4% in controls).
In conclusion, fibrosis is not responsible for the early cardiac dysfunction induced by ISO.
04.076 - WHAT ARE THE MECHANISMS INVOLVED IN THE HYPOTENSIVE EFFECT OF EUGENIA
DYSENTERICA LEAF EXTRACT?
PATRICIA FIDELIS DE OLIVEIRA GREGOLINI1; SAMANTA APARECIDA CASTRO 1; LUIZ BORGES BISPODA-SILVA2; VICTOR HUGO MARTINS DE MIRANDA1; MARIANA SPINARDI1; JULIANA IRANI FRATUCCI DE
GOBBI1
1.UNIVERSIDADE ESTADUAL PAULISTA-UNESP, BOTUCATU, SP, BRASIL; 2.UNIVERSIDADE FEDERAL
DE UBERLÂNDIA-UFU, UBERLÂNDIA, MG, BRASIL.
Eugenia dysenterica (ED) leaves have been used to treat cardiovascular disorders; however, there
is no scientific data that describe their effect on the cardiovascular system. We investigated the
effect of lyophilized aqueous extract of ED leaves (ED-LE) on mean arterial pressure (MAP) in
anaesthetized normotensive rats as well as the possible mechanisms involved. Venous and arterial
access was established for drugs/ED-LE administration and MAP recording, respectively. Doseeffect curves were obtained in the same animal (dose: 1-60 mg/kg).The effects of ED-LE (15
mg/kg) were evaluated in the absence and presence of atropine (AT, 3 mg/kg); L-NAME (10
mg/kg), hexamethonium (HEX, 20 mg/kg) and indomethacin (IND, 20 mg/kg). Intravenous ED-LE
promoted reduction in MAP in a dose-dependent manner (ED1: 0.9±1%; ED6: -8±2%; ED10: -15±3%;
ED15: -33±5%; ED20: -42±6%, ED30: -52±5%, ED60: 100%). AT and HEX administration did not
change the hypotensive effect of ED-LE (-40±8% vs. -40±7%, before and after AT; -27±6% vs. -21±3%
before and after HEX). The hypotensive effect of ED-LE was manifested even after endotheliumderived vasodilators production inhibition by L-NAME (-31±4% vs. -34±7%, before and after) and
IND (-19±1% vs. -24±3% before and after). Thus, muscarinic receptors activation, autonomic
nervous system modulation, NO or PGI2 synthesis are not involved in ED-LE-induced hypotesion
and other mechanisms remain to be elucidated.
04.077 - CORONARY REACTIVITY IMPAIRMENT OF RATS WITH EPILEPSY
PAULA RODRIGUES VITORINO; KARINA PEREIRA GOMES; POLIANA PERES GHAZALE; BEATRIZ
PACHECO DE SOUZA; ELIZABETH PEREIRA MENDES; CARLOS HENRIQUE DE CASTRO; DIEGO BASILE
COLUGNATI; GUSTAVO RODRIGUES PEDRINO; ALINE PRISCILA PANSANI
This study aims to evaluate the coronary reactivity of rats submitted to the pilocarpine model of
epilepsy (PILO). Wistar male rats (240-280g) were divided in two groups: control (CN) (n=5) and
epilepsy (EP) (n=5). EP group was submitted to PILO (350 mg/Kg, i.p. of pilocarpine, preceded by
1mg/Kg, s.c. of methylscopolamine) and CN were composed by rats submitted to PILO without
status epilepticus. Both EP and CN rats were monitored by Video (24 hrs/7 days/week). After 60
days after the first spontaneous and recurrent seizure, EP group and their matched CN group,
were sacrificed and hearts dissected to perform the adapted Langendorff (constant flow) protocol
for isolated hearts perfused by Krebs-Hanseleit solution. After 30 minutes of stabilization, the
heart was treated with bradykinin (BK) (10ˉ⁸, 10ˉ⁷, 10ˉ⁶ and 10ˉ⁵M) and sodium nitroprusside
(NPS) (10ˉ⁶, 10ˉ⁵, 10ˉ⁴ and 10ˉ³M) randomly administered (in bolus) into the perfusion system. A
10 min of washout was realized between drugs administration. We evaluated heart rate, dP / dt
maximum and minimum, intraventricular systolic and diastolic pressure and perfusion pressure
(PP), in both before and after bolus of BK and NPS. The results showed decreased coronary
36
relaxation mediated by BK 10ˉ⁶M in EP (12 ± 3%) compared to CN (22 ± 2%) and BK 10ˉ⁵M in EP
(15.6 ± 3%) compare to CN (26.9 ± 2). Thus, our results indicate endothelial dysfunction of the
coronary arteries of rats with epilepsy.
04.078 - EFFECTS OF MIRABEGRON ON BODY FAT AND AORTA REACTIVITY IN OBESE MICE
CARMEM PERES VALGAS DA SILVA1; FABIANO BERALDI CALMASINI2; EDUARDO COSTA ALEXANDRE2;
TUANY ZAMBROTI CANDIDO2; EDSON ANTUNES2; FABIOLA TAUFIC IGLESIAS2; ANGELINA ZANESCO1
1.UNESP, RIO CLARO, SP, BRASIL; 2.UNICAMP, CAMPINAS, SP, BRASIL.
Objective: Analyse the effect of Mirabegron (Mira), a selective β₃-AR agonist on body weight (BW),
Epididymal fat mass (EFM), Food intake (FI), lipid profile (LP) and vascular reactivity (VR) of aorta
in the presence (PVAT+) and absence (PVAT-) of PVAT in obese mice. Methods: C57BL/6J mice
were divided into four groups: vehicle control group (CV) and treated with Mira (CM) for 2 weeks
(10 mg/kg by gavage administration); obese fed with high-fat diet (12 weeks) and treated with
vehicle (OV) and Mira (OM). Acetylcholine (ACh 100pM to 30 μM) and serotonin (5HT 1nM to 30μM)
were used in aorta in PVAT+ and PVAT-. Results: Treatment with Mira for 2 weeks increased FI by
21% while reduced BW (7%) and EFM (17%) only in OM compared to OV. The fat diet increased total
cholesterol (58%) and LDL-C (150%) compared to CV and Mira reverted these alterations. PVAT+
altered the maximum response (Emax) to ACh in obese groups (OV PVAT- 66.8±1.6/OV PVAT+
41.2±4.4; OM PVAT- 70.4±3.9/OM PVAT+ 38±5.7) and no alterations with Mira treatment. PVAT+
increased Emax of 5HT (mN/mm) in aortic rings in CV, OV and OM groups (CV PVAT- 1.3±0.2/PVAT+
2.7±0.5, OV PVAT- 5±0.1/PVAT+ 2.9±0.5, OM PVAT- 1.9±0.1/OM PVAT+ 3.7±0.6). Mira treatment
increased Emax in all the experimental groups in PVAT- (100% for CM compared to CV and 18% OM
compared to OV).
Conclusion: Treatment with Mira was effective in reducing the EFM in obese mice and improved
LP without affecting vascular response.
04.079 - MESENCHYMAL STROMAL CELLS ATTENUATE HEMODYNAMIC CHANGES AND LUNG
REMODELING IN MONOCROTALINE-INDUCED PULMONARY ARTERIAL HYPERTENSION
LUCAS DE MENDONCA1; NATHANE SANTANA FELIX1; NATALIA GOES BLANCO1; JAQUELINE SOARES
DA SILVA1; TATIANA PAULA TEIXEIRA FERREIRA2; FERNANDA FERREIRA CRUZ1; SORAIA CARVALHO
ABREU1; NAZARETH DE NOVAES ROCHA1; PATRÍCIA MARQUES SILVA2; VERA LÚCIA CAPELOZZI3;
GISELE ZAPATA SUDO1; PATRICIA RIEKEN MACEDO ROCCO1; PEDRO LEME SILVA1
1.UNIVERIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL; 2.FUNDAÇÃO OSVALDO
CRUZ, RIO DE JANEIRO, RJ, BRASIL; 3.UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRASIL.
RATIONALE: Pulmonary arterial hypertension (PAH) is an incurable disease that progresses to
severe right heart failure. Numerous therapies have proven useful in decreasing pulmonary arterial
pressure, but an effective therapy that can modify long-term outcomes is lacking. We sought to
evaluate the effects of mesenchymal stromal cell (MSC) therapy on hemodynamics, lung
remodeling and levels of growth factors in monocrotaline-induced pulmonary arterial hypertension
(PAH).
METHODS: Wistar rats (n=45) were randomly divided into two groups. In the PAH group, rats
received intraperitoneal monocrotaline (60 mg/kg), whereas controls received saline (SAL). After
14 days, both groups received either MSCs (105 cells) or SAL intravenously.
RESULTS: At day 28, PAH-MSC animals, compared to PAH-SAL, exhibited lower right ventricular
systolic pressure (RVSP) (28.8 mmHg vs. 38.8 mmHg, p<0.017), lung collagen content (26.82 µg
vs. 36.32 µg, p<0.017), and mRNA expression of type I and type III procollagens and TGF-beta from
fibroblasts. Similarly, PAH-MSC rats had improved lung histology and reduced levels of vascular
endothelial growth factor (VEGF) in lung tissue. VEGF correlated positively with RVSP (r=0.88,
p<0.001).
Conclusion: In this model of PAH, MSCs attenuated hemodynamic variables associated with PAH,
reduced lung and vascular fibrosis, and modulated expression of VEGF in lung tissue.
04.080 - THE ROLE OF PERIVASCULAR ADIPOSE TISSUE IN AORTA FROM TRAINED OBESE MICE
ANDRESSA SILVA SOUSA; AMANDA CHRISTINE SPONTON; MARIA ANDRÉIA DELBIN
The aim of this study was to investigate the role of perivascular adipose tissue (PVAT) in aorta
from trained obese mice. Male mice were divided into: control sedentary (c-SD);trained (cTR);sedentary obese(o-SD);trained obese (o-TR). For 16 weeks,the o-SD and o-TR groups were fed
with high-fat diet to induce obesity. After 8 weeks,the c-TR and o-TR groups initiated the aerobic
exercise training (AET) on treadmill (60-70% of maximum speed),5 days/week,60 min/session until
the end of the protocol.Concentration-response curves to acetylcholine (ACh), sodium
nitroprusside (SNP), thromboxane A2 (U46619) and endothelin-1 (ET-1) were obtained in thoracic
aorta in the absence or presence of PVAT. Protein expressions were determined only in PVAT.
Biochemical parameters were analyzed systemically (CEUA:3278-1). As expected, the high-fat diet
increased the body weight,epididymal fat,glycemia,total cholesterol,triglycerides,resistin and
leptin in o-SD and o-TR.The amount of aortic PVAT was also markedly increased.The PVAT did not
modify the relaxation response to ACh in c-SD and c-TR groups.However the PVAT reduced the
maximal response and potency values in o-SD group. We observed an increased iNOS protein
expression in PVAT from o-SD.AET was effective in preventing these alterations in o-TR group.No
changes were observed to SNP, U46619 and ET-1, and eNOS, Cu/Zn-SOD, Mn-SOD and EC-SOD
protein expressions. In conclusion, the AET prevented the endothelial dysfunction caused by PVAT
in thoracic aorta from obese mice.
04.081 - ICV ANGIOTENSIN-(1-7) DECREASED TNF-α AND INCREASED IL-10 HYPOTHALAMUS IN
(MREN2)27 TRANSGENIC HYPERTENSIVE RATS
LUCAS MIRANDA KANGUSSU; MARILENE LUZIA DE OLIVEIRA; ROBSON AUGUSTO SOUZA DOS SANTOS;
MARIA JOSE CAMPAGNOLE-SANTOS
FEDERAL UNIVERSITY OF MINAS GERAIS, BELO HORIZONTE, MG, BRASIL.
Hypertensive rats subjected to chronic intracerebroventricular (ICV) infusion of angiotensin-(1-7)
[Ang-(1-7)] presented attenuation of arterial hypertension. In the present study we evaluated
whether chronic increase in Ang-(1-7) in the brain modulates inflammatory mediators in the
hypothalamus of the transgenic hypertensive rats [(mRen2)27; TGR]. Sprague Dawley (SD) and
TGR were subjected to 14 days of ICV infusion of Ang-(1-7) (200 ng/h) or 0.9% sterile saline
(0.5µl/h) through osmotic mini-pumps. Cytokine levels were evaluated through ELISA assay and
enzymes of RAS were measured by fluorimetric assays. As expected, levels of pro-inflammatory
cytokines (IL-1α, IL-6 and TNF-α) were increased in TGR (48±3.5 pg/mg, 61±2.7 pg/mg, 76±4.3
pg/mg, respectively) as compared to SD rats (23±1.8 pg/mg, 38±3.4 pg/mg, 39±3.5 pg/mg,
respectively), while IL-10 was not altered. Interestingly, ICV infusion of Ang-(1-7) reduced levels
of TNF-α (48±3.4 pg/mg vs 76±4.3 pg/mg in untreated TGR) and increased the levels of IL-10
(32±2.6 pg/mg vs 19±1.2 pg/mg in untreated TGR), without affecting IL-1α or IL-6 levels. No
difference was found in ACE activity in plasma, on the other hand, the increased ACE activity in
the hypothalamus of TGR (207±25.5 vs 173±13.0 in SD rats) was significantly reduced (134±7.9) by
ICV infusion of Ang-(1-7). These data show that long term increase in Ang-(1-7) levels in the brain
modulates inflammatory mediators in the hypothalamus, suggesting a possible additional
mechanism for Ang-(1-7) antihypertensive action in the central nervous system.
04.082 - DIMINAZENE ENHANCES STABLE PLAQUE PHENOTYPE OF ATHEROSCLEROTIC PLAQUES
RAFAELA FERNANDES DA SILVA1; RODRIGO A. FRAGA SILVA2; FABRIZIO MONTECUCCO3; FABIANA P.
COSTA-FRAGA2; FRANÇOIS MACH3; MOHAN K. RAIZADA4; ROBSON A.S. SANTOS1; NIKOLAOS
STERGIOPULOS2
1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.SWISS FEDERAL
INSTITUTE OF TECHNOLOGY, LAUSANNE, SUICA; 3.UNIVERSITY OF GENEVA, GENEVA, SUICA;
4.UNIVERSITY OF FLORIDA, GAINESVILLE, ESTADOS UNIDOS.
Angiotensin Ang-(1-7) has atheroprotective actions. Angiotensin-converting enzyme 2 (ACE2), an
enzyme which breaks-down Ang II and forms Ang-(1-7), reduces atherosclerotic plaque size and
vulnerability. Here we investigated the actions of diminazene, a recently developed ACE2
activator compound, in a mouse model of vulnerable atherosclerotic plaque. Methods and results:
ApoE gene-deleted mice were fed with western-type diet for 11 weeks. The formation of stable
and vulnerable plaques was induced in the carotid artery by the insertion of perivascular cast,
imposing the vessel locally to oscillatory (OSS) and low shear stress (LSS), respectively. The mice
were treated with diminazene (15 mg/Kg/day) or vehicle for the last 3 weeks. ACE2 was strongly
expressed in the aortic root and LSS-induced carotid plaques, but poorly expressed in the OSSinduced carotid plaques. Diminazene treatment did not change the lesion size, but ameliorated
the composition of aortic root and unstable carotid plaques, by increasing collagen content and
decreasing both MMP-9 expression and macrophage infiltration. Interestingly, these beneficial
effects were not observed in the OSS-induced plaque. Additionally, diminazene treatment
decreased circulating cytokines and chemokines levels. Conclusion: ACE2, a major enzyme of the
renin angiotensin system, is distinctively expressed in atherosclerotic plaques, depending on the
local pattern of shear stress. Diminazene treatment may enhance the stability of atherosclerotic
plaques.
04.083 - BLOOD PRESSURE EFFECT OF CHRONIC INTRACEREBROVENTRICULAR INFUSION OF
ALAMANDINE IN TRANSGENIC HYPERTENSIVE RATS
LUCAS ARAUJO CASTRO E SOUZA; ROBSON AUGUSTO SOUZA DOS SANTOS; MARIA JOSE
CAMPAGNOLE-SANTOS
Alamandine, a new peptide of the renin-angiotensin system (RAS) acts through the selective
receptor-MrgD and, heretofore, is shown to induce similar actions of angiotensin-(1-7). In this
study we investigated the cardiovascular effects of alamandine in transgenic (mRen2)27
hypertensive rats (TG). TG rats with different ages, instrumented with telemetry probes for
arterial pressure (AP) measurement, underwent 28 days of lateral ventricle (ICV) infusion of
alamandine (50, 200 or 800 ng/h) or saline (Control; 0.25 μL/h) through osmotic mini-pumps.
Alamandine infusion, at 200 ng/h, in TG rats with 9-10 and 15-16 weeks old did not alter the
increase in MAP that is observed in these ages. However, 12 weeks old TG rats (Baseline MAP
day: 162±8 mmHg and night: 150±9 mmHg) that received 200 ng/h of alamandine showed a small
but significant decrease in MAP both during the day (∆MAP: -16 ±8 mmHg) and night (∆MAP: -12±7
mmHg) from the first week of infusion. TG control presented, in contrast, the expected increase
in MAP during the infusion period (Day: ∆MAP: +18±5 mmHg; Night: ∆MAP: +12±4
mmHg). Alamandine infusion did not alter baseline heart rate or the circadian rhythm of MAP or
heart rate. TG rats that received 50 and 800 ng/h of alamandine did not present significant
changes in MAP. Our data show that chronic infusion of alamandine in the brain attenuates the
hypertension in these TG rats, suggesting an alternative route for the RAS in the central control
of AP.
04.084 - THE CAGE SWITCH STRESS AFFECTS THE CARDIOVASCULAR RESPONSES BUT NOT
ALTER THE METABOLIC RESPONSES
CRISTIANE AMORIM DE PAULA; FERNANDA RIBEIRO MARINS; PRISCILA GUIMARAES; LETÍCIA M SOUZA
CORDEIRO; MARCO ANTONIO FONTES
The dorsomedial hypothalamic region (DMH) plays a key role in the organization of the
cardiovascular (CV) response to acute emotional stress. Inhibition of DMH and/or blockade of
excitatory amino acid (EAA) receptors in this region attenuate the physiological responses evoked
by stress. However, all these effects have been explored in a single stress model (air-stress). We
evaluate the contribution of NMDA receptors in the DMH on the CV response evoked by cage switch
stress (CSs). Wistar rats received guide cannulas into DMH. Seven days after, a cannula was
inserted into femoral artery for HR and BP recording. After 24 hs, bilateral injections (100nL) of
vehicle (saline 0.9%, n=7), the GABAA agonist muscimol (100pmol, n=8) or the NMDA antagonist
AP-5 (100pmol, n=6) were performed into DMH; ten min later, rats were submitted to CSs. An
additional control group was also tested (no injection, intact group, n=9). In the intact group and
in the vehicle group CSs evoked a tachycardic response (Δ 119±12 bpm and 121±11 bpm)
accompanied by large increases in BP (Δ 60±2 mmHg and Δ 60±3 bpm, respectively). The
tachycardic and pressor responses were markedly reduced by muscimol and AP-5 (musc: ΔHR:
62±7 bpm and Δ MAP: 37±4 mmHg; AP-5: Δ HR: 31±7 bpm and Δ MAP: 39±3 mmHg). Corticosterone
and glucose levels were unaffected by CSs. The DMH is also important for controlling CV responses
in other forms of acute emotional stress. Part of this response involves activation of NMDA EAA
receptors
37
that the neurotransmission of this response is not dependent on glutamatergic receptors. We
evaluate the involvement of purinergic P2 receptors, alone or combined with glutamatergic
receptors, in the A5 area in cardiovascular responses of chemoreflex in awake rats. We observed
that the microinjection of the P2 agonist α,β-methylene ATP into A5 area (1.25nmol/100nl)
induced increase in mean arterial pressure (+0.0±0.5 vs +25±3mmHg) and heart rate (+0.9±2 vs
+56±10bpm; n=8). However, bilateral microinjection of the P2 antagonist PPADS (0.5nmol/100nl)
produced no changes in pressor (+38±6 vs +39±6mmHg) and bradycardic responses (-253±8 vs 237±25bpm) of the chemoreflex (n=5). The sequential microinjection of PPADS (0.25nmol/50nl)
and kinurenic acid (2nmol/50nl) into the A5 area produced no changes in pressor (+49±3 vs
+41±4mmHg) and bradycardic (-252±23 vs -259±28bpm) responses of chemoreflex (n=6). Our
results indicate that purinergic P2 receptors in the A5 area participate in the control of the arterial
pressure and heart rate. However, purinergic receptors, alone or combined with glutamatergic
receptors, do not participate in the integration of cardiovascular responses of the chemoreflex in
awake rats.
04.088 - NADPH OXIDASE REGULATION BY POLYCYSTIN-1 IN CARDIOMYOCYTES
ADRIANA CÓRDOVA-CASANOVA1; IVONNE OLMEDO1; GINA SANCHEZ1; PAULINA DONOSO1; ZULLY
PEDROZO2
1.FACULTAD DE MEDICINA, UNIVERSIDAD DE CHILE, SANTIAGO DE CHILE, CHILE; 2.FACULTAD DE
MEDICINA Y ACCDIS, UNIVERSIDAD DE CHILE, SANTIAGO DE CHILE, CHILE.
NADPH oxidase 2 (Nox2), is one of the most important sources of reactive oxygen species (ROS) in
the cardiomyocyte and plays a crucial role in cardiac physiology and pathology. Mechanical stretch
activates Nox2 and increases ROS generation but the mechanosensor implicated in this activation
is unknown. Polycystin-1 (PC1), a transmembrane protein that acts as a mechanosensor in
different cells, is also expressed in cardiomyocytes but its physiological function is not fully
understood. To investigate the role of PC1 in the regulation of Nox2 activity we measured nitrated
proteins, as an indication of ROS generation, in neonatal rat ventricular cardiomyocytes
transfected with siRNA specific to PC1 or in heart homogenates from PC1 knockout mice.
We observed that the decreased PC1 expression in the neonatal cardiomyocytes transfected with
the PC1 siRNA caused a significant increase in nitrated proteins. Apocynin, a Nox2 inhibitor,
prevented this increase. Heart homogenates from PC1 KO mice also showed an increase in nitrated
proteins as compared to controls, confirming the data obtained in cardiomyocytes. Our results
suggest that PC-1 is a new regulator of Nox2 in cardiomyocytes which inhibits Nox2, and prevents
the generation of ROS in baseline conditions.
04.089 - TACHYCARDIA INDUCED BY ACUTE EMOTIONAL STRESS IS MEDIATED BY NMDA
RECEPTORS IN THE RAPHE PALLIDUS
TIAGO GOMES PAULA DOS REIS COELHO; NATHÁLIA BITTENCOUT DE CAMPOS ORLANDO; THAIS
ZIELKE DIAS CARDOSO; NATÁLIA LIMA SANTOS MACHADO; MARCO ANTONIO FONTES
UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MA, BRASIL.
Sympathetic premotor neurons in the raphe pallidus (RPa) are essentially recruited to promote
the tachycardia induced by acute emotional stress. RPa receives afferent projections from the
region of dorsomedial hypothalamus (DMH), a key region controlling the sympathetic response to
stress. However, the specific receptors in the RPa involved in this response are largely unknown.
We evaluated the contribution of NMDA receptors in mediating the tachycardia evoked by acute
emotional stress. The anatomical projections from DMH to RPa were also reevaluated. Under
anesthesia, Wistar rats (300 ± 50g) were prepared for injections into the RPa, recording of blood
pressure (BP) and heart rate (HR). After injection of vehicle or the NMDA receptor antagonist, AP5 (dose) into RPa (n=3-6), the animal was placed in a restraining tube and an air jet (10 L/min)
was directed to the head of the animal. The tachycardia evoked by air stress was largely
attenuated by the preinjection of AP-5 into the RPa (Δ HR: 130 ± 19 bpm after vehicle vs. 45 ± 7
bpm after AP-5, P=0,012). No significant changes in BP were observed. Retrograde axonal tracing
injection into RPa showed a highest density of neurons retrogradely labeled located at dorsal area
of hypothalamus. Current experiments suggest that NMDA receptors in the RPa play an important
role in mediating the tachycardia, evoked by acute stress. Support: FAPEMIG, CNPq, CAPES.
04.085 - EFFECTS OF SULFORAPHANE ON RIGHT VENTRICULAR REMODELING IN A MODEL OF
PULMONARY ARTERIAL HYPERTENSION
ADRIANA CONZATTI; RAFAEL COLOMBO; RAFAELA SIQUEIRA; VANESSA DUARTE ORTIZ; TÂNIA
REGINA GATELLI FERNANDES; ADRIANE BELLÓ-KLEIN; ALEX SANDER DA ROSA ARAUJO
Introduction: Pulmonary arterial hypertension (PAH) is a serious disease of unknown etiology.
Oxidative stress may be involved in this disease and reactive oxygen species can be interesting
therapeutic targets. The Sulforaphane (SFN) is an isothiocyanate found in cruciferous vegetables
with a potential induction of antioxidant enzymes. This study analyzed influence of SFN on heart
function of rats with monocrotaline-induced PAH. Methods: Male Wistar rats (~250g)
(n=~10/group) were divided into four groups: Control (C); Control+SFN (CS); Monocrotaline (M);
Monocrotaline+SFN (MS). Treatment with SFN (2.5 mg/kg by gavage) started on the 7th day after
the HAP induction. After 21 days of protocol, rats were anesthetized and was performed
echocardiography, catheterization and euthanasia of animals. Statistical analysis: Two-way
ANOVA with Student Newman-Keuls post test. Differences were considered significant if P<0.05.
Ethics Committee number 26270. Results: The MS group showed a reduction in systolic (~14%) and
diastolic (~18%) pressure of the right ventricle (RV) compared to the group M. There was no change
in stroke volume and cardiac output. The Tricuspid Annular Plane Systolic Excursion (TAPSE),
echocardiographic measure of RV ejection fraction, was higher in the MS group compared to the
M group (~17%). Pulmonary vascular resistance was higher in M group compared to the others.
Conclusion: The date suggest that SFN treatment can be improve cardiac function in rats with
PAH.
04.090 - LIFE TIME INCREASE IN ANGIOTENSIN-(1-7) IN THE BRAIN DECREASES BLOOD PRESSURE
DANIELE TEIXEIRA ALVES1; TODIRAS MIHAIL2; MICHAEL BADER2; ROBSON AUGUSTO SOUZA DOS
SANTOS3; MARIA JOSE CAMPAGNOLE-SANTOS3
1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.MDC, BERLIN,
ALEMANHA; 3.UFMG, BELO HORIZONTE, MG, BRASIL.
In the present study, we characterized the cardiovascular function of transgenic rats (TGR) that
exhibit increased Ang-(1-7) production selectively in the brain. Sprague-Dawley (CTL) and TGR
were instrumented with telemetry probes for blood pressure (BP) monitoring. The levels of
circulating and brain Ang-(1-7) were determined by RIA. Baroreflex bradycardia (phenilephrine),
cardiac autonomic tonus(blockade of β-adrenergic and muscarinic receptors) and vascular
sympathetic tonus(blockade of α1-adrenergic receptors) were evaluated using the Biopac. Urinary
parameters were evaluated using metabolic cages and plasma vasopressin levels were determined
by ELISA. TGR showed lower basal mean BP(98±2 mmHg vs 105±1.5 mmHg in CTL during the day
and during the night 102± 2mmHg vs 108±2 mmHg in CTL) without significant change in baseline
HR. TGR presented increased levels of Ang-(1-7) in the hypothalamus(29±3 pg/mg of protein vs
19±2 pg/mg of protein in CTL) without significant change in plasma levels. TGR presented an
increased cardiac sympathetic tonus(156 ± 6 beats/min vs 94±11 beats/min in CTL).TGR showed
an increased vascular sympathetic tonus(-23±3 mmHg vs-12 ±2 mmHg in CTL). No significant
changes in the baroreflex bradycardia was observed. The urinary volume was lower in TGR (9±1
ml/24 hours vs 12±1 ml/24 hours in CTL). TGR showed an increase in plasma AVP (5.5±1 pg/ml vs
2.8±1 pg/ml in CTL).These data suggest that TGR as an important change in BP is being maintained
by compensatory mechanisms that involve increased plasma AVP levels and peripheral and cardiac
sympathetic tonus.
04.086 - PROTEASOME INHIBITION PROTECTS MITOCHONDRIA DURING CARDIAC ISCHEMIA
REPERFUSION
GINA SANCHEZ; IVONNE OLMEDO; LUIS MONTECINOS; GONZALO PINO; ZULLY PEDROZO; PAULINA
DONOSO
UNIVERSIDAD DE CHILE, SANTIAGO, CHILE.
Myocardial ischemia-reperfusion (IR) causes mitochondrial dysfunction. Since partial inhibition of
the proteasome protects the heart from IR injury we investigated the effect of partial inhibition
of the proteasome during IR on mitochondrial morphology and function. We also evaluated the
content of mitofusin 2 and DRP-1, two proteins involved in the regulation of mitochondrial network
dynamic.
Isolated rat hearts were perfused with Krebs-Henseleit solution with or without the proteasome
inhibitor MLN 9708, 100 nM, for 10 minutes prior to 30 minutes of ischemia followed by 60 minutes
of reperfusion. Mitochondrial morphology was assessed by electron microscopy in heart sections.
In mitochondrial fractions we evaluated oxygen consumption and the content of mitofusin 2 and
DRP-1 in western blots.
We found that IR greatly disturbed mitochondrial morphology, decreased oxygen consumption to
20 % of the control value and decreased mitofusin 2 and DRP-1 content by 50%. MLN 9708, at a
concentration that only inhibits the chymotrypsin-like activity of the proteasome by 50 %, largely
prevented the alterations on mitochondrial morphology, normalized oxygen consumption and
prevented the decreased in mitofusin 2, a protein involved in mitochondrial fusion, but not that
of DRP-1, a protein involved in mitochondrial fission.
We conclude that the beneficial effect of proteasome inhibition during IR is due in part to the
preservation of the mitochondrial function.
04.091 - RENAL DENERVATION PRODUCES NON UNIFORM EFFECTS ON THE ARTERIAL
BARORECEPTOR FUNCTION IN GOLDBLATT RENOVASCULAR HYPERTENSION
GISELE SILVERIO LINCEVICIUS; CAROLINE GUSSON SHIMOURA; ERIKA EMY NISHI; CASSIA TOLEDO
BERGAMASCHI; RUY RIBEIRO CAMPOS JUNIOR
Sympathetic nerve activity is increased in renovascular hypertension. Renal denervation (DnX) has
shown promise in the treatment of patients with resistant hypertension to drug therapy, although
the mechanisms underlying reduction in blood pressure remains uncertain.We evaluated the
effects of unilateral renal denervation (H+DnX) in efferent post-ganglionic sympathetic nerve
activity and arterial baroreceptor reflex sensitivity to renal (rSNA) and lumbar (lSNA) sympathetic
nerves in Goldblatt hypertensive rats (2K1C). The baroreceptor sensitivity to heart rate was also
evaluated. Denervation of clipped kidney was performed 5 weeks after clipping. The rSNA and
baroreflex control to rSNA was performed in the innervated contralateral kidneys. Ten days after
DnX, blood pressure (BP) was significantly reduced in the 2K1C rats compared to 2K1C
undenervated (H). DnX normalized rSNA (C:109±7,n=14; H:150±8,n=12; H+DnX:89±7, pps n=8) and
lSNA (C:68±5,n=8; H:105±7,n=12; H+DnX:54±4,pps n=9) in the 2K1C rats. DnX significantly
improved the baroreceptor function for rSNA (gain in 20mmHg variation=C:-2.7±0.3, n=11; H:0.9±0.1, n=7; H+DnX:-1.7±0.2, pps/mmHg n=7) and bradycardic response (C:-2.5±0.1, n=7; H:1.6±0.07, n=5; H+DnX:-2.6±0.2, bpm/mmHg n=3), but not for lSNA in 2K1C rats. The results show
that renal denervation normalized sympathetic vasomotor activity accompanied by a non uniform
improvement in the arterial baroreceptor function. Whether the baroreceptor function sensitivity
improvement induced by DnX is a cause or a consequence of BP reduction remains to be
determined.
04.087 - PURINERGIC AND GLUTAMATERGIC RECEPTORS IN THE A5 AREA ARE NOT INVOLVED IN
CARDIOVASCULAR RESPONSES OF CHEMOREFLEX IN AWAKE RATS
SILVIA AMARAL ZEBRAL; ANDREA SIQUEIRA HAIBARA
Previous studies demonstrated that the integrity of noradrenergic neurons in the A5 area is
essential for full expression of the chemoreflex pressor response in awake rats, and also showed
04.092 - SYMPATHETIC VASOMOTOR ACTIVATION TO DIFFERENT TARGETS IN A MODEL OF
HYPERTENSION INDUCED BY NITRIC OXIDE SYNTHASE BLOCKADE
LYSIEN IVANIA ZAMBRANO1; ROBERTO BRAZ PONTES JR1; MICHELLE LOUVAES GARCIA2; ERIKA EMY
NISHI2; RUY RIBEIRO CAMPOS JUNIOR2; CASSIA TOLEDO BERGAMASCHI2
1.UNIFESP/ UNAH, SAO PAULO, SP, BRASIL; 2.UNIFESP, SAO PAULO, SP, BRASIL.
High blood pressure is a global public health problem. Several factors are involved in the
generation and maintenance of hypertension, such as increased sympathetic nerve activity (SNA),
although controversies have been reported. Our aims were to evaluate sympathetic activation to
two targets, the kidneys (rSNA) and other visceral bed, the splanchnic (sSNA), in animals with
hypertension induced by nitric oxide synthase blockade (L-NAME). Wistar rats were treated with
L-NAME (20mg/kg/day, once a day, for 8 days). L-NAME-treated group presented increased blood
pressure (154±13 mmHg, N=16), compared with control group (102±2 mmHg, N= 10). Both rSNA
(156±37.4 spikes/s, N=16) and sSNA (145±42.7 spike/s, N=16) were higher in L-NAME-treated
group, compared with control group (111±20.6, N=10 and 63±20.6 spikes/s, N=10, respectively).
Taken altogether, our data show that the hypertension induced by nitric oxide synthase blockade
is characterized by sympathoexcitation to more than one bed and that can be involved in the
generation and/or maintenance of high blood pressure.
04.093 - EFEITO DA INIBIÇÃO DO MICRORNA-34C* NA HIPERTROFIA DE CARDIOMIÓCITO EM
CULTURA CELULAR
CLARA NOBREGA; EDILAMAR MENEZES OLIVEIRA
ESCOLA DE EDUCAÇÃO FÍSICA E ESPORTE USP, SAO PAULO, SP, BRASIL.
Resumo:
Hipertrofia cardíaca (HC) fisiológica ativa a via de PI3K-AKT-mTOR-p70S6K, porém animais
nocautes para essa via submetidos ao treinamento físico (TF) aeróbio apresentam HC, apontando
à participação de outras vias de sinalização, como do complexo eIF4E. O TF diminui 98% a
expressão do microRNA-34c*(miR-34c*) no coração e análise in silico, mostra o gene do eIF4E como
alvo deste miR. Assim, investigamos se a inibição do miR-34c* aumenta a expressão gênica do
eIF4E levando à hipertrofia de cardiomiócitos em cultura. As células foram transfectadas com
antagomiR-34c*(AmiR-34c*) e miRmimic-34c*(mmiR-34c*), a expressão do miR-34c* e do eIF4E foi
analisada em RT-PCR, proteica por Western-blot e hipertrofia por imunohistoquímica. A expressão
do miR-34c* diminuiu linearmente com a concentração de Amir-34c*, sendo o pico de
71%(50nM;p<0,01), enquanto aumentou 41000%(50nM;p<0,01) no grupo tratado com o mmiR-34c*
vs. C. O tratamento com Amir-34c*(50nM) aumentou a expressão gênica do
eIF4E(9,2%;p<0,01), proteica do p-eIF4BP1/eIF4BP1(46%;p<0,01), e não altera os genes eIF4BP1
e eIF4G. O mmiR-34c* diminuiu expressão gênica do eIF4E (21,5%;p<0,01). Portanto, o AmiR-34c*
foi eficaz na inibição do miR-34c* aumentando a expressão gênica do seu alvo, eIF4E, e proteica
do p-eIF4BP1. Dados que, somados à manutenção da expressão gênica do eIF4BP1 e eIF4G, genes
diretamente envolvidos na regulação da atividade do eIF4E, sinalizam que o miR-34c* pode ser um
potente regulador da HC fisiológica em cardiomiócitos.
04.094 - ROLE OF RENAL SENSORY NERVES IN RENAL FUNCTION AND BLOOD PRESSURE
REGULATION UNDER PHYSIOLOGICAL AND HYPERTENSIVE STATES
JOSE FRANCISCO MELO JR1; ROBERTO BRAZ PONTES JR1; ADRIANA C GIRARDI2; RUY RIBEIRO
CAMPOS JUNIOR1; CASSIA TOLEDO BERGAMASCHI1; ERIKA EMY NISHI1
1.UNIFESP, SAO PAULO, SP, BRASIL; 2.INCOR, SAO PAULO, SP, BRASIL.
Renal sympathetic nerves play a crucial role in urinary flow, renin secretion and sodium excretion.
Physiological studies have shown that sensory innervation in the kidney also influence renal
function by modulating the sympathetic nerve activity. Our aim was to evaluate the mechanism
by which renal afferents influence the renal function in the contralateral kidney. Electrical
stimulation (15V, 40Hz, 1h) of renal afferents was performed in normotensive male Wistar rats
and the activity of Na+/H+ exchanger (NHE3) was evaluated in the contralateral kidney. Moreover,
we evaluated the effectiveness of a novel method of selective denervation of sensory fibers with
application of periaxonal capsaicin (33mM, 15 min) in a model of renovascular hypertension (2
kidney-1 clip, 2K1C). Stimulation of renal afferents lead to ~10mmHg increase in arterial pressure
(AP) and 63% increase in urinary flow in the contralateral kidney that were not associated with
changes in NHE3 activity (sham group n=5; stimulated group n=6). Ten days after afferent
denervation of the ischemic kidney in 2K1C rats (n=4), mean AP, diastolic AP and systolic AP were
decreased by 13% compared with sham denervated group (n=4), indicating that renal afferents
contribute partially to the increase in blood pressure in this model. Ongoing experiments from our
laboratory are directed toward investigating the role of renal afferents in renal function under
physiological and hypertertensive states.
04.095 - ESTROGEN RECEPTORS EFFECTS IN THE RVLM OF RENOVASCULAR HYPERTENSIVE RATS
NADIA OLIVEIRA MARUYAMA; HEDER FRANK GIANOTTO ESTRELA; THAÍS FABIANA LUCAS; CATARINA
SEGRETI PORTO; CASSIA TOLEDO BERGAMASCHI; RUY RIBEIRO CAMPOS JUNIOR
It is well stablished that 17β-estradiol (E2) is a cardiovascular protector in part via reduction of
sympathetic vasomotor activity. However, there is no information regarding the estrogen
receptors (ESR1and GPER) actions in the rostroventrolateral medulla (RVLM) in the 2kidney-1clip
(2K1C) hypertension model. Thus, we investigate the role of estrogen receptors within the RVLM
on sympathetic activity in 2K1C male Wistar rats. Protein expression of ESR1 and GPER within the
RVLM were avaluated throught Western blot assay. Bilateral microinjection of E2 (ESR1 and GPER
agonist), G1 (GPER agonist) and ICI182,780 (ESR1 antagonist) microinjections into the RVLM were
performed in control (C) and 2K1C rats, six weeks after renal clipping. Renal sympathetic nerve
activity (rSNA) and mean arterial blood pressure (MAP) were recorded in anesthetized rats. A
significant increase in ESR1(2K1C 300%n=7) and GPER (2K1C 800%n=8) expression was found in 2K1C rats. Losartan 2K1C treated significantly reduced GPER expression in the RVLM (300%n=5). E2
microinjection decreased MAP(CΔ-23.5±2.3n=6; and 2K1CΔ-16.6±1.5mmHg n=6) and rSNA (CΔ40.8±8n=6 and 2K1CΔ-19.1±2.3ppsn=6) the response was significantly reduced in the 2K1C.
However, G1 into the RVLM induced larger MAP increase (C,Δ 22±6.6 n=5 and 2K1C
Δ41.83±4.4mmHg n=6) and rSNA (CΔ41±6.4 n=5 and HΔ 65.8±8.5pps n=5), in 2K1C. No difference
was found, however, in reponse to ICI microinjection between groups. E2 apparently has opposed
functions into the RVLM depending on which receptor (ESR1 or GPER) is predominantly active in
2K1C.
04.096 - INVOLVEMENT OF OXIDATIVE STRESS AND NITRIC OXIDE IN LUNG PARENCHYMA OF
FEMALE RATS WITH PULMONARY ARTERIAL HYPERTENSION
RAFAELA SIQUEIRA; RAFAEL COLOMBO; ADRIANA CONZATTI; CRISTINA CAMPOS CARRARO;
ALEXANDRE LUZ DE CASTRO; TÂNIA REGINA GATELLI FERNANDES; ALEX SANDER DA ROSA ARAUJO;
ADRIANE BELLÓ-KLEIN
Pulmonary arterial hypertension (PAH) is a progressive disease, which affects more women than
men. The main of this study was to analyze parameters related to oxidative stress in the lungs of
rats with PAH. Female Wistar rats were divided into four groups (n = 4-8): sham (S), S +
monocrotaline (SM), ovariectomy (O) O + MCT (OM). The ovariectomy was performed when the
animals were 60 days old. After 7 days of ovariectomy or surgery simulation, PAH was induced by
monocrotaline (MCT) (60 mg/kg i.p.). The animals were kept for 21 days after MCT injection.
Then, the animals were anesthetized (ketamine - 90 mg/kg and xylazine - 10 mg/kg i.p.), killed
by cervical dislocation and the lungs were removed for subsequent analysis. It was evaluated
NADPH oxidase, superoxide dismutase (SOD) and nitric oxide synthase (NOS) activity, nitrites and
lipid peroxidation by TBARS. MCT promoted an increase in NADPH oxidase, SOD and NOS activity,
as well as decreased concentration of nitrite in the lung parenchyma (P < 0.05). There was
observed an increase (73%) in lipid peroxidation in OM compared to SM. It is suggested that MCT
have promoted an imbalance in the nitric oxide metabolism, as measured by the increase in NADPH
oxidase and NOS activity. Both enzymes are involved in the formation of peroxynitrite, a major
cytotoxic agent. Even more, the absence of estrogen in the OM animals increased damage to
membrane lipids in the lung parenchyma.
04.097 - SOLANUM LYCOCARPUM (LOBEIRA) AMELIORATES VASODILATATION IN AORTA AND
CORONARY VASOS OF DIABETIC RATS
ELIZABETH PEREIRA MENDES; ALLANCER DIVINO CARVALHO NUNES; PEDRO HENRIQUE ALVES;
CARLOS HENRIQUE DE CASTRO; DIEGO BASILE COLUGNATI; KÁTIA FLÁVIA FERNANDES
UFG, GOIANIA, GO, BRASIL.
38
Previous results showed that Solanum lycocarpum St. Hill (SL) commonly known as “Lobeira”
decreases the glicemia in diabetic rats. The aim of the present study was to evaluate the cardiac
mechanical function and the reactivity of aorta rings in diabetc rats (DR) treated with powder
lobeira. Methods and Results: Diabetes mellitus model was induced by administering a single dose
of alloxan monohydrate (150mg/kg, i.p.) in male wistar rats. Rats were deprived of food for 24
hours before induction of diabetes. After 3 days, rats with hyperglycemia (fasting blood glucose
220–260mg/100ml) were used. Solution of the lobeira (0,2 g/5 mL) fruit powder was administrated
by oral gavage once daily by 8 days. To avaliate the cardiac mechanical function, hearts were
isolated and perfused at constant pressure with Krebs-Ringer solution following Langendorff
methodology. After stabilization intraventricular pressure and dP/dt were measured.
Intraventricular pressure (IP) and dP/dt were decreased in untreated rats. In isolated arteries,
Acetilcolina (10 -9 to 10-5 M) induced a concentration-dependent relaxation of pre-contracted
aortic rings. Vasorelaxations at treated rats showed a tendency for an increase. Conclusions:
These results showed that aqueous suspension of the lobeira, at least in the dose used, have good
effects in cardiac mechanical functions and aortic response. Further studies are necessary to
clarify this effect and possible mechanisms involved in this effect.
04.098 - INCREASED RENAL SYMPATHETIC NERVE ACTIVITY UP-REGULATES NA+/CL- COTRANSPORTER (NCC) ON CLIPPED AND NON-CLIPPED KIDNEYS IN RENOVASCULAR
HYPERTENSION
CAROLINE GUSSON SHIMOURA1; GISELE SILVERIO LINCEVICIUS1; ELIZABETH BARBOSA OLIVEIRASALES1; ROBERTO BRAZ PONTES JR1; ADRIANA C GIRARDI2; CASSIA TOLEDO BERGAMASCHI1; RUY
RIBEIRO CAMPOS JUNIOR1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO - UNIFESP, SAO PAULO, SP, BRASIL; 2.INSTITUTO DO
CORAÇÃO - INCOR - UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRASIL.
Increased renal sympathetic nerve activity (RSNA) has an important role in the development and
maintenance of renovascular hypertension (2K1C). In 2K1C model, both kidneys, clipped (C) and
non-clipped (NC), acts to maintain sodium and water balance. However, the effects of RSNA on
sodium renal transporters and renal function remain unclear. In the present study, we evaluated
the glomerular filtration rate (GFR), urinary flow (UF) and Na+-Cl- cotransporter (NCC) protein
expression in C and NC kidneys. The effects of unilateral renal denervation (RDN) in such
parameters were also evaluated. A silver clip was implanted in the left renal artery in male Wistar
rats and four weeks after clipping, the C kidney was denervated. All experiments were performed
2 weeks after RDN. The GFR was decreased only in C kidney in 2K1C rats compared to control
group (Ctr), RDN didn’t change GFR (Ctr2.2±0.13; C0.6±0.14; RDN0.76 ±0.16 ml/min). In NC
kidney, UF was increased compared to Ctr and was significantly decreased by RDN
(Ctr0.019±0.004; C0.079±0.02; RDN0.028 ±0.008 ml/min). NCC protein expression was increased
in both kidneys in 2K1C rats and RDN decreased this expression only in NC kidneys (Ctr100±7.2;
NC137±9.9; RDN60±15; C148%C±9.2). Altogether, the results suggest that RSNA has an important
role in modulating NCC protein expression in NC kidney in 2K1C. However, RDN did not alter renal
function parameters.
04.099 - PREGNANCY ALTERS BAROREFLEX FUNCTION IN WISTAR RATS
LUIZ HENRIQUE NATALI; DANIELA DE SOUZA SILVA; JÉSSICA ANTONINI TROIANO; CRISTINA
ANTONIALI
UNESP - ARAÇATUBA, ARACATUBA, SP, BRASIL.
Late pregnancy in rats is associated with marked decrease in mean arterial pressure. Arterial
baroreflex rapidly resets when arterial pressure is lowered, however, whether baroreflex
sensitivity is changed in late pregnancy has been a subject of controversy. The aim of our study
was to compare the baroreflex sensitivity between virgin Wistar rats (VW) and pregnant Wistar
(PW) rats. VW (estrous day, n=8) and PW (19-20th day, n=8) were anesthetized and catheters were
inserted into femoral artery and vein for measurement of arterial pressure and drug infusion.
Mean arterial pressure (MAP) and heart rate (HR) were recorded before and after infusion of
different doses of phenylephrine (PE, 0.5, 1.0, 2.0, 4.0, 8.0 mg/kg) and sodium nitroprusside (NPS,
5, 10, 15, 35, 50 mg/kg). Baroreflex sensitivity was calculated by variation of HR and MAP
(ΔHR/ΔMAP). Results were compared between groups (Student t test, p<0.05). Basal MAP, before
PE or SNP infusion, was significantly reduced (p <0.01) in PW (95.6 ± 3 mmHg) when compared to
VW (110.2 ± 2.4 mmHg). Baseline HR was not different (p>0.05) between groups (PW: 393.9 ±
16.3; VW: 383.2 ± 9.9 bpm). As basal levels of MAP were reduced in PW, changes in MAP after PE
or SNP infusion were calculated as ΔMAP. The ΔMAP and ΔHR after PE doses were not different
(p>0.05) between groups. However, baroreflex sensitivity was reduced (p <0.05) to all tested
doses of NPS in PW. Our results demonstrated that baroreflex function in response to decreases
in blood pressure was blunted in PW.
04.100 - CORRELATION BETWEEN STRESS AND HEART RATE VARIABILITY IN ASSYMPTOMATIC
ADULTS
MAYARA SILVEIRA BIANCHIM; MARCIA CARVALHO GARCIA; VICTOR ZUNIGA DOURADO; EVANDRO
SPERANDIO; REGINA CELIA SPADARI
UNIFESP, SANTOS, SP, BRASIL.
This study aims to investigate if there is any association between HRV and stress markers. Ninetyfour asymptomatic, 48-78 year-old volunteers, 31 male and 63 female, participated in the study.
Anthropometric parameters, blood glucose and lipids, salivary cortisol concentration (SC) and
electrocardiogram (ECG) were determined. HRV indi-ces were estimated by Kubios software. The
mean heart rate was 69±10 bpm and the R-R interval was 889±136 ms. The SC was high in the
morning and low in the even-ing. Obesity, tobacco smoking, age and triglycerides were positively
correlated with SC. Some HRV indexes were identified, through linear regression analysis, as
predictors of SC and perceived stress, even after adjusting for age, sex and other risk factors. In
a multiple regression analyses SC was negatively correlated with RMSSD (β: -1.193), sample
entropy (β: -0.691), SDRR (β: -0.275), and HF (-0.339); and perceived stress with LF (β: -0.258).
Concluding, an association between stress and low HRV was iden-tified in the present study,
regardless of age, sex and other risk factors for CVD. Thus, stress management can be effective
in the prevention of cardiovascular diseases, con-sidering its important correlation with HRV.
04.101 - VAGUS NERVE STIMULATION EFFECTS ON SYMPATHETIC NERVE ACTIVITY AND
INFLAMMATION IN A RHEUMATOID ARTHRITIS MODEL IN RATS
DANIEL P MARTINS DIAS; GABRIEL S BASSI; ALEXANDRE KANASHIRO; CARLOS ALBERTO AGUIAR
SILVA; THIAGO M CUNHA; RUBENS FAZAN; HELIO CESAR SALGADO
FACULDADE DE MEDICINA DE RIBEIRÃO PRETO - UNIVERSIDADE DE SÃO PAULO, RIBEIRAO PRETO,
SP, BRASIL.
Several studies have shown that electrical activation of neural pathways can be used for treating
pathophysiological disorders, for instance, rheumatoid arthritis. The effects of electrical
stimulation of the vagus nerve (VNS) on renal sympathetic nerve activity (RSNA) and inflammatory
responses were evaluated in a rheumatoid arthritis model. Male Wistar rats (280 g body mass)
were anesthetized and implanted with a catheter into the femoral artery combined with an
electrode around the left vagus nerve. A separate set of rats was implanted, also, with an
electrode around the left renal sympathetic nerve. Rats were kept anesthetized and had the RSNA
recorded at baseline conditions during 10 minutes, followed by a single session (10-minutes long)
of VNS under two conditions: A) 5 Hz, 0.1 ms, 1 V; and, B) 20 Hz; 0.1 ms; 3 V. Another set of rats
was subjected to VNS followed, immediately, by injection of zymosan into the femorotibial joint
to elicit rheumatoid arthritis; the synovial fluid was collected for neutrophil count six hours later.
VNS under A condition elicited no changes in RSNA but elicited an anti-inflammatory effect
characterized by decreased (-44±8% vs. control rats) neutrophil count. VNS under B condition
increased RSNA (27±13% vs. baseline condition) without anti-inflammatory effect. Thus, these
findings show that mild VNS elicits an anti-inflammatory effect without noticeable change in RSNA.
04.102 - EXPERIMENTAL GESTATIONAL HYPOTHYROIDISM REDUCES INTRINSIC HEART RATE IN
ADULT OFFSPRING RATS
IURA GONZALEZ NOGUEIRA-ALVES1; SAULO ARAÚJO MAKERRAN-LOUREIRO1; ANTONIO LUCAS SILVAFILHO1; VANESSA CIBELLE BARBOZA DE CARVALHO1; DANIELLE PEREIRA GAUJAC1; EDÊNIA DA
39
CUNHA MENEZES1; PATRÍCIA RABELO DOS SANTOS1; DANIEL P MARTINS DIAS 2; VALTER JOVINIANO
SANTANA-FILHO1; DANIEL BADAUÊ-PASSOS, JR.1
1.UNIVERSIDADE FEDERAL DE SERGIPE, SÃO CRISTÓVÃO, SE, BRASIL; 2.FACULDADE DE MEDICINA
DE RIBEIRÃO PRETO/USP, RIBEIRÃO PRETO, SP, BRASIL.
Gestational hypothyroidism is a prevalent disorder in pregnant women. We aimed to investigate
the effect of experimental gestational hypothyroidism (EGH) in cardiac chronotropic activity in
male offspring on postnatal day (PND) 120 during pharmacological challenges (methylatropine
and/or propranol). EGH was induced by methimazole (MMI) 0.02% in drinking water from day 9 of
gestation until birth. Offspring from MMI-treated dams (OMTD, n = 6) or water-treated dams
(OWTD, n = 6) had femoral veins and arteries surgically assessed. OMTD had higher mean (120.4 ±
2.7 vs 109.6 ± 2.1 mmHg, p < 0.001), systolic (148.2 ± 3.0 vs 134.9 ± 3.4 mmHg, p < 0.001) and
diastolic arterial pressure (98.3 ± 2.2 vs 89.5 ± 2.0 mmHg, p < 0.001) when compared to OWTD.
Both groups (OMTD and OWTD) similarly responded to parasympathetic or sympathetic
pharmacological blockade. However, double pharmacological autonomic blockade reveled that
OMTD presented a lower intrinsic heart rate when compared to OWTD (376.2 ± 8.4 vs 400.4 ± 6.2
bpm, p < 0.05). These findings suggest a critical role of maternal THs in development and function
of fetal sinoatrial node and/or in a cardiac conduction system of heart.
O exercício físico regular aeróbio reduz a pressão arterial média (PAM) e modula a sensibili-dade
barorreflexa de ratos anestesiados com hipertensão renovascular. O objetivo deste trabalho foi
estudar a sensibilidade barorreflexa em ratos normotensos (N) e hipertensos (H), sedentários (S)
e treinados (T), com livre movimentação. Ratos Fischer (180-200g, n=5-6/grupo) foram
anestesiados e submetidos à cirurgia 2R1C (2 rins-1 clipe) ou fictícia(CEUA: 146/2013). 48 h após
a cirurgia os animais foram divididos em grupos sedentário e treinado (natação, sem carga, 1
h/dia, 5 dias/semana, durante 4 semanas) e após 4 semanas foram anestesiados e canulados
(artéria e veia femorais). 48h depois, em animais com livre movi-mentação, foram registrados os
parâmetros cardiovasculares (PAM e frequência cardíaca, FC) e realizado o teste do barorreflexo
por meio da injeção intravenosa de fenilefrina (FE: 20 µg/mL/kg) e nitroprussiato de sódio (NPS:
30 µg/mL/kg).O grupo HS apresentou uma maior PAM (185±4vs NS 109±1 mmHg) e FC (439±12 vs
NS 371±8 bpm), e um menor índice de sensibilidade do barorreflexo no teste com NPS (FC/PAM
1,25±0,1 vs NS 5,7±1,4 bpm/mmHg), sendo tanto a FC (HT 411±11 bpm)e o FC/PAM(HT: 4,8±0,6
bpm/mmHg) melhorados após a natação. Não houve diferenças entre os grupos para o teste com
FE. Os resultados mostram que o treinamento foi eficiente em melhorar a sensibilidade do barorreflexo no teste com NPS nos animais 2R1C.
Palavras chaves: Goldblatt, exercício físico, barorreflexo
04.103 - GREEN TEA IMPROVES CARDIOVASCULAR FUNCTION IN NITRIC OXIDE SYNTHESIS
BLOCKADE HYPERTENSION
MICHELLE LOUVAES GARCIA1; ROBERTO BRAZ PONTES JR1; ERIKA EMY NISHI1; FLAVIA KAZUE IBUKI2;
VANESSA OLIVEIRA1; FERNANDO NEVES NOGUEIRA2; MARIA DO CARMO FRANCO1; LILA MISSAE
OYAMA1; RUY RIBEIRO CAMPOS JUNIOR1; CASSIA TOLEDO BERGAMASCHI1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE DE SÃO PAULO,
SÃO PAULO, SP, BRASIL.
Oxidative stress is a key mediator in the maintenance of sympathoexcitation and hypertension in
human and experimental models. Green tea (GT) is widely known as a potent antioxidant. We
aimed to evaluate the effects of GT in a model of hypertension induced by nitric oxide synthase
inhibitor (L-Name; 20 mg/kg/day, vo, for 2 weeks) in male Wistar rats. After 1th week of L-Name
treatment, animals received GT ad libitum for 1 week (9,6g/L). By the end of treatment period
blood pressure (BP), heart rate (HR), baroreflex sensitivity and renal sympathetic nerve activity
(rSNA). The systemic oxidative stress conditions were also assessed. L-name treated animals
presented an increase in BP (165±2 mmHg) compared with control rats (103±1 mmHg) and GT
treatment reduced hypertension (119±1 mmHg). Hypertensive animals showed a higher rSNA
(161±12 pps) compared to control group (97±2 pps) and GT treatment s also decreased this
parameter in the hipertensive group (125±5 pps). Arterial baroreceptor function was improved in
hypertensive rats after GT treatment. Moreover, GT improved oxidative stress condition
systemically in hypertensive rats. Taken altogether, GT treatment was able to reduce BP,
improving baroreflex sensitivity and reducing rSNA probably due the antioxidants properties.
04.108 - TAMOXIFENO REDUZ PRESSÃO ARTERIAL E FREQUÊNCIA CARDÍACA EM MODELO
EXPERIMENTAL DE MENOPAUSA
SARAH CRISTINA F. FREITAS; CAMILA GOMES DA SILVA; FILIPE FERNANDES CONTI; HUMBERTO
DELLÊ; KATIA DE ANGELIS; KARINA FRANCO VARGAS; IRIS CALLADO SANCHES
UNIVERSIDADE NOVE DE JUNHO, SAO PAULO, SP, BRASIL.
Introdução: A privação estrogênica após a menopausa eleva o risco cardiovascular. Entretanto, a
reposição hormonal apresenta efeitos adversos, como efeitos pró-neoplásicos. Considerando que
os efeitos cardioprotetores do estrógeno são dependentes de seus receptores, a hipótese deste
estudo é a de que moléculas moduladoras desses receptores, como o tamoxifeno, podem ser
cardioprotetores promissores. Objetivo: Avaliar o efeito do tamoxifeno, modulador seletivo de
receptores de estrógeno, sobre parâmetros hemodinâmicos (pressão arterial, PA e frequência
cardíaca, FC) em modelo experimental de menopausa. Material e Métodos: Para isso, 24 ratas
Wistar (200-220g) foram divididas em 4 grupos (n=6 em cada): fêmeas controles (C) e tratadas
com tamoxifeno (FT), ooforectomizadas controles (OC) e tratadas com tamoxifeno (OT). Após 8
semanas de protocolo, os animais foram canulados na artéria carótida para registro direto de PA
(2KHz, CODAS). O teste estatístico Anova Two Way foi aplicado para comparação de dados, seguido
do teste Student Newman-Keulls, p<0,05. Resultados: O grupo OT apresentou redução na PA
sistólica (C: 133±0,29; FT: 125±5,35; OC: 129±2,77; OT: 114±7,56 mmHg), na PA média (C:
116±1,20; FT: 110±5,39; OC: 112±3,17; OT: 99±7,05 mmHg) e na FC (C: 375±7,55; FT: 389±6,84;
OC: 385±17,96; OT: 360±7,75 bpm). Conclusão: Os resultados deste estudo indicam que os efeitos
cardioprotetores do estrógeno são dependentes de seus receptores e o tamoxifeno pode ser
promissor para a cardioproteção no período pós-menopausa.
04.104 - CARDIAC AUTONOMIC MODULATION AT REST IS HIGHER DURING SPONTANEOUS
BREATHING COMPARED TO FIXED RESPIRATORY FREQUENCY IN HEALTHY INDIVIDUALS
GABRIEL DIAS RODRIGUES; THIAGO RODRIGUES GONÇALVES; PEDRO PAULO DA SILVA SOARES
FEDERAL FLUMINENSE UNIVERSITY, NITERÓI, RJ, BRASIL.
Background: It is well recognized that the breathing pattern has a strong influence on heart rate
(HR) and blood pressure dynamics, however the contributions of fixed or spontaneous breathing
on heart rate variability (HRV) or baroreflex sensitivity (BRS) remains unclear. Aim: Investigate
the impact of spontaneous respiratory frequency on HRV and BRS compared to controlled
breathing. Methods: 11 healthy volunteers participated in protocol of 5min record blood pressure
continuously (Finometer, NL) at sitting with spontaneous breathing (SITspo), or sitting (SITcon)
positions with controlled breathing set at 0.25Hz. HRV was analyzed in the time domain as the
mean of R-R intervals (RR) and the root mean square of successive differences in the R-R times
series (rMSSD), in frequency domain the high (HF, 0.15-0.40Hz) and low-frequency (LF, 0.040.15Hz) components were presented in normalized units. The BRS obtained by the αLF were
calculated. Results: R-R (843.8 ±151.7 vs 799.0 ±151.6ms; p=0.03) and rMSSD (53.39 ±31.96 vs
45.13 ±26.27ms; p=0.01) are higher in SITspo than SITcon. HF (22.54 ±14.04 vs 28.99 ±20.77 n.u;
p=0.31) and LF (45.42 ±7.9 vs 44.57 ±21.05 n.u; p=0.07) are not differ between in SITspo and
SITcon. To BRS the αLF (12.7 ±5.1 vs 9.0 ±2.1ms/mmHg; p<0.01) were modified between SITspo
and STIcon. Conclusion: VFC and BRS are higher in spontaneous than controlled breathing.
04.105 - UTILIZAÇÃO DA MICROSCOPIA DE DESFOCALIZAÇÃO PARA ANÁLISE DA CONTRAÇÃO DE
CARDIOMIÓCITOS TRATADOS COM ANGIOTENSINAS II E 1-7
SÉRGIO RICARDO ALUOTTO SCALZO JÚNIOR; ITAMAR COUTO GUEDES DE JESUS; ANA PAULA ALVES;
MARINA DE SOUZA LADEIRA; LUIZ ORLANDO LADEIRA; ROBSON AUGUSTO SOUZA DOS SANTOS;
OSCAR NASSIF DE MESQUITA; UBIRAJARA AGERO BATISTA; SILVIA CAROLINA GUATIMOSIM FONSECA
A mudança no padrão de contração fornece informações importantes sobre a fisiologia dos
cardiomiócitos. Desta forma, a captura de imagens por microscopia de desfocalização representa
uma poderosa ferramenta para identificar alterações no padrão de contração. O objetivo desse
trabalho foi investigar alterações no padrão de contração por meio da microscopia de
desfocalização em cardiomiócitos tratados com dois peptídeos, distintos, que possuem ações
antagônicas no coração, a Angiotensina (Ang)-(1-7) e Ang-II. Para isso, foram retirados corações
de ratos wistar com idade entre 1-3 dias, imersos em meio HBSS com tripsina e picotados até uma
espessura de 2 mm. Os cardiomiócitos isolados foram plaqueados e tratados com 100nM de Ang-II
ou 1-7, durante 36 horas. As imagens foram obtidas utilizando microscopia de desfocalização, com
uma taxa de captura de 30hz/10min,sendo comparadas cronologicamente. A seqüência binária
gerada foi transformada em um gráfico de área por tempo. Como resultado, houve um aumento
significativo da área de contração nos grupos Ang-(1-7) (22,05%) e Ang-II (41,60%) quando
comparados ao controle. Além disso, a frequência de contração foi significativamente maior
apenas no grupo tratado com Ang-II. Esses resultados mostram que a microscopia de
desfocalização forneceu dados distintos do padrão de comportamento celular dos cardiomiócitos
tratados com Ang-II e 1-7, apresentando ser uma técnica promissora para este tipo de análise.
04.106 - CAROTID BODY DENERVATION PREVENTS HYPERTENSION IN PROTEIN-RESTRICTED
OFFSPRING
JOSÉ LUIZ DE BRITO ALVES; VIVIANE DE OLIVEIRA NOGUEIRA; MONIQUE ASSIS DE VASCONCELOS
BARROS; ALMIR GONÇALVES WANDERLEY; CAROL GOIS LEANDRO; JOÃO HENRIQUE DA COSTA SILVA
UNIVERSIDADE FEDERAL DE PERNAMBUCO, VITORIA DE SANTO ANTAO, PE, BRASIL.
Evidences has indicates that carotid body (CB) chemoreceptors exhibit an important function in
pathophysiology of hypertension. So, we investigated the involvement of the CB in the genesis of
hypertension in protein-restricted rats. Wistar rats were subjected to control (NP, 17% of casein)
or low-protein (LP, 8% of casein) diets during gestation and lactation. At 28-day-old, the carotid
sinus nerves were surgically denervated under general anaesthesia (NP-cbd and LP-cbd). Shamoperated rats (NP-sham and LP-sham) underwent the same surgical procedures to expose the CB
but the carotid sinus nerves were left intact. Direct measurements of arterial pressure (AP) and
heart rate were recorded from wakeful 90-day-old male offspring. After, It was performed the
variability of the AP systolic (SAP) and pulse interval (PI), with compounents quantified as low
frequency (LF=0.2–0.75Hz) and high frequency (HF=0.75-3.0Hz). The protocol was approved by
the Ethical Committee of the UFPE (2013-81). CB denervation in LP rats significantly reduced the
AP (LP-sham: 109±3 vs. LP-cbd: 88±5mmHg,p<0,05). When compared to the LP rats, the
improvement in AP of LP-cbd rats was associated with decrease of the LF of the SAP (LP-sham:
7±1 vs. LP-cbd: 3±0,5mmHg²,p<0.05), However, HF components of the SAP and LF/HF ratio of the
PI were similar between groups. Thus, the CB are, in part responsible for the elevated sympathetic
and arterial blood pressure in rats subjected to protein restriction during pregnancy and lactation.
04.107 - NATAÇÃO MELHORA O BARORREFLEXO EVOCADO NO MODELO EXPERIMENTAL DE
HIPERTENSÃO RENOVASCULAR
MARCONE RODRIGUES DA SILVA; ANDREIA CARVALHO ALZAMORA; LEONARDO MÁXIMO CARDOSO;
LISANDRA BRANDINO DE OLIVEIRA
04.109 - SODIUM INTAKE REGULATES VASOPRESSIN GENE EXPRESSION DIFFERENTLY IN THE PVN
OF WISTAR AND SHR
NATALIA RIBEIRO; LAIALI JURDI CHAAR; VAGNER ROBERTO ANTUNES
UNIVERSIDADE DE SÃO PAULO, SÃO PAULO, SP, BRASIL.
It is known that osmotic challenges leads to an increase in blood pressure that is associate with
sympathoexcitation, and the paraventricular nucleus of hypothalamus (PVN) plays an important
role in this response. Previous studies of our lab have shown that arginine vasopressin (AVP)
microinjected into the PVN is able to increase lumbar sympathetic nerve activity and blood
pressure of normohydrated rats. Here, we sought to evaluate whether the salt-sensitivity of SHR
could be due to a differential modulation of AVP mRNA into the PVN when compared with Wistar
rats. Methods & Results: Institutional approved ethical committee protocol N° 111- Male SHR and
Wistar (300 g). Were offered NaCl 2% in replacement of the drinking water, and kept in metabolic
cages for one, four or seven days to evaluate hydroelectrolytic parameters. At the end, the
animals were decapitated and the PVN was microdissected for RNA extraction and qPCR
experiments for AVP. The results have shown an upregulation of AVP mRNA expression in the PVN
of Wistar with 4 days of salt-intake exposure (3,63± 0.94 a.u., n=8), and returning to basal level
(1.00 ± 0.14) when animals were kept for 7 days to salt overload (1.38±0.56 a.u. n=4). On the
other hand, in SHR the AVP mRNA expression was higher only in the group of 7 days of salt-intake
(4.12±1 a.u. n=4). In conclusion, we can suggest that AVP mRNA expression in the PVN of saltloaded animals is time-dependent and differently modulated in Wistar and SHR.
THEME 05 - GASTROINTESTINAL PHYSIOLOGY
05.009 - LIGHT-EMITTING DIODES AMELIORATES INTESTINAL TRANSIT IN A MOUSE MODEL OF
ULCERATIVE COLITIS
MÔNICA DE OLIVEIRA BELÉM; THALITA MASSI CARLOS; GIOVANA MARIA MARQUES DE ANDRADE;
CARLA FABIANA SOUZA GUAZELLI; VICTOR FATTORI; DARI DE OLIVEIRA TOGINHO FILHO; IVAN
FREDERICO LUPIANO DIAS; WALDICEU APARECIDO VERRI; EDUARDO JOSÉ DE ALMEIDA ARAÚJO
UNIVERSIDADE ESTADUAL DE LONDRINA, LONDRINA, PR, BRASIL.
Ulcerative colitis (UC) alters the intestinal transit (IT) because the inflammation. Light emitting
diodes (LED) have anti-inflammatory and tissue healing effect. Our objective was to evaluate the
effect of LED therapy (940nm, 4J/cm2) on the IT of mice with UC. Adult Swiss mice were assigned
into 5 groups (n=8/group/assay): Salin, SalinLED, UC, UCLED and UCPred. UC was induced on UC,
UCLED and UCPred groups by administration of 7.5% acetic acid (pH 2.5) via a rectal catheter.
UCPred group was treated with prednisolone (5mg/kg/day) orally during 5 days before the UC
induction. The LED therapy, was performed using light with 940nm wavelength, 45nm bandwidth,
4J/cm2 intensity and 9,5mW optical power. Ten hours after of UC induction, it was administered
carmine red (6%) and methylcellulose (0.5%) orally and we observed three times of elimination of
first red pelet: ≤2h; between 2h and 7h; ≥7h. The assay was carried out in duplicate. Statistical
analysis was performed by two-way ANOVA followed by Newman-Keuls, p<0.05 was considered
significant. All Saline and SalineLED mice showed normal IT (≤2h). All mice from the UC group had
delayed IT, predominantly ≥7h. LED therapy ameliorated the IT in 35.71% of the UC mice compared
to untreated UC group (p<0.05). The same effect was observed in 30.76% of UC mice compared to
UCPred group (p<0.05). In conclusion, LED therapy induces the recovery of IT in a mouse model
of acetic acid-induced ulcerative colitis.
05.010 - EFFECT OF ANGIOTENSIN (1-7) ON TRYPTOPHAN JEJUNAL ABSORPTION IN MURINE
ELIZABETH LAGE BORGES; ROBSON AUGUSTO SOUZA DOS SANTOS; PATRICIA BATISTA LIMA;
ANTONIO AUGUSTO PELOSO; MARILENE LUZIA DE OLIVEIRA; JAMIL SILVANO DE OLIVEIRA; WALKYRIA
SAMPAIO; RAFAEL TEMPONI RUOCCOLO; GISELE MAIA ETELVINO; ANDERSON JOSÉ FERREIRA
OBJECTIVES: To investigate the effect of angiotensin-(1-7) [Ang-(1-7)] (2.5 nmol/kg) administered
(iv) and the effect of an angiotensin-converting enzyme 2 (ACE2) activator, diminazene aceturate
(DIZE) (1 mg/kg, iv) on jejunal tryptophan (Trp) absorption.
METHODS: Male Wistar rats (210±20 g, n=5 each group) and WT (FVBN) or Mas KO mice (27.2±3,8
g, n=6-9 each group) were anesthetized and submitted to midline laparotomy to exposed and
isolate 20 cm of jejunal loop. Tyrode solution (pH 8) containing Trp (0. 25 mg %) was infused (0.5
mL and 0.15 min−1, for rats and mice respectively) into the jejunal loop and samples were taken
at 10-min intervals during the 40-min experiment.
RESULTS: Results were expressed by difference between influx and efflux. Ang-(1-7) increased
Trp absorption when compared to the control group (0.13±0.03 vs. 0.23±0.0 mg%, p<0.05). The
increase on Trp absorption after DIZE treatment did not reach significance, p = 0.09 (0.13±0.03
vs.0.18±0.01). The ACE2 activity in the effluent of jejunal perfusion and the expression of ACE2
in the tissue of small intestine was higher in the group that received Ang-(1-7) when compared
with the control (13.6±1.5 vs.50.5±4.1and 100±0.0 vs.193.7±17.0%, p<0.05, respectively). The
effect of Ang-(1-7) on Trp absorption were blunted in Mas Ko mice as compared to WT (1.7±3.0 vs
9.8±2.6 in WT).
CONCLUSION: The results suggest the participation of Ang-(1-7) in modulating intestinal Trp
absorption which may be at least partially dependent on ACE2 activation.
05.011 - GOJI BERRY’S POTENTIAL INHIBITION OF PANCREATIC α-AMYLASE AS AN ACTION
MECHANISM FOR THE POSSIBLE EFFECTS AGAINST OBESITY AND DIABETES
IZABELA N. TEIXEIRA; EDUARDO I. REZENDE; BRENDA L.B. COSTA; RAYSSA O. SANTOS; ISABELA
COELHO DE CASTRO; LUCIANA L.S. PEREIRA
UNIVERSIDADE FEDERAL DE LAVRAS, LAVRAS, MG, BRASIL.
The Goji Berry (GB) (Lyciumbarbarum) is an oriental fruit that became a mania in the Western
world. The weight loss promise is the main reason of the growing consumption. In this context,
the aim is to evaluate the inhibitory potential of GB extracts on the pancreatic α-amylase enzyme
in attempt to suggest an action mechanism that justifies the weight loss and the hypoglycemic
activity, what may be associated from glucose decreased uptake in the duodenum. So, it presents
itself as an auxiliary tool in treatment of diabetes and obesity. The α-amylase activity was
determined in order kinetics 4 times (10, 20, 30 and 40 min). The enzyme inhibition percentage
was calculated by the expression: “%inibition = (acontrole - aamostra/acontrole)x100”. The
inhibition percentage was obtained by the slope of the graphic line (absorbance x time) of enzyme
activity control test (without sample) and enzyme + inhibitor (with sample). The slope is due the
per minute product formation rate and the inhibitor presence causes decrease in that inclination.
The absorbance values were converted to product by a mmol of glucose standard curve. It was
observed that the aqueous extract of GB strongly inhibited α-amylase 1:80 was the chosen
concentration to determine the inhibition μmol/min in a safe range for calculation (40-80%
inhibition). The hypoglycemic and slimming effects of GB can be relate do the strong inhibition of
pancreatic α amylase
THEME 06 - CELULAR PHYSIOLOGY
06.007 - CONVERSION OF HUMAN MACROPHAGES INTO FOAM CELLS REDUCES THEIR
PROINFLAMMATORY RESPONSES TO CLASSICAL M1-POLARIZING ACTIVATION
RAFAELA FERNANDES DA SILVA1; JANI LAPPALAINEN2; MIRIAM LEE-RUECKERT2; PETRI T. KOVANEN2
1.UNIVERSIDADE FEDERAL DE MINAS GERAIS, BELO HORIZONTE, MG, BRASIL; 2.WIHURI,
HELSINQUE, FINLANDIA.
In atherosclerotic lesions, cholesterol-laden macrophage foam cells are formed and exposed to
M1 and M2-polarizing factors; however, their effects on the pro- and anti-inflammatory potential
of foam cells are not known. Here, we investigated the effects these factors on the expression of
inflammation-related genes in human macrophage foam cells. Methods and Results: Human
monocytes were differentiated into macrophages in the presence of M-CSF, and then converted
into cholesteryl ester-loaded foam cells by incubation with acetyl-LDL. Finally, the generated
foam cells were polarized into M1 phenotype by classical activation with LPS and IFN-g or into M2
phenotype by alternative activation with IL-4. The non-loaded M1- and M2-polarized macrophages
were characterized by typical upregulation and down-regulation of several key proinflammatory
genes, respectively. However, the proinflammatory response of the foam cells to the classical M1
activation was weaker than that of the non-loaded macrophages, as demonstrated by reduced
upregulation of genes expressing TNFA, IL1B, CXCL8, and COX2, and reduced stimulation of TNFA secretion. In contrast, alternative M2 activation of either non-loaded or cholesterol-loaded
macrophages failed to lead to significant suppression of the expression of proinflammatory
cytokines. Conclusion: Conversion of cultured human macrophages into foam cells suppresses their
proinflammatory responses to M1-polarizing factors.
06.008 - 830 NM LASER PHOTOTHERAPY DECREASED ALPHA 1-ADRENOCEPTORS IN BONE
HEALING
TALES LYRA DE OLIVEIRA1; ELIANE NUNES LEITE2; IONÉIA ALVES GOMES2; NÁVYLLA CANDEIA DE
MEDEIROS1; LÉIA CARDOSO DE SOUSA3; ROBINSON SABINO-SILVA3; ELAINE FÁVARO-PIPI1
1.UNIVERSIDADE FEDERAL DE ALAGOAS - UFAL, MACEIÓ, AL, BRASIL; 2.FACULDADE DE ALAGOAS FAL, MACEIÓ, AL, BRASIL; 3.UNIVERSIDADE FEDERAL DE UBERLÂNDIA - UFU, UBERLÂNDIA, MG,
BRASIL.
The sympathetic nervous system (SNS) activation causes osteopenia via beta 2-adrenoceptor
signaling. Although, alpha 1-adrenoceptors are present in bone, their functions in bone healing
have been less studied. The aim of this study was to measure the pattern of alpha 1-adrenoceptors
expression during bone healing in bone defects treated with low-level laser therapy (LLLT). Wistar
rats were divided in: SHAM (bone defect without treatment) and LLLT (bone defect treated with
LLLT 830 nm, 30mW, 15J/cm²). Noncritical size bone defects were surgically created at the upper
third of the tibia under anesthesia [ketamine (40 mg/kg, ip) and xylazine (10 mg/kg, ip)]. Laser
irradiation started 24 h post-surgery and was performed for 3 and 6 sessions, with an interval of
48 h. The tibias were removed for histologic (HE) and immunohistochemistry (to alpha 1adrenoceptors) analyses. CEUA-FAL: 14/2013. The histological results revealed increase of primary
bone deposition after 3 and 6 sessions with LLLT than SHAM. The quantification of alpha 1adrenoceptors expression revealed that LLLT produced a decrease (p < 0.05) of alpha 1adrenoceptors at day 7th and 13th post-surgery. Taken together, our results indicate that lower
alpha 1-adrenoceptors protein expression in tibia after LLLT treatment contributes to bone repair.
THEME 07 - COMPARATIVE PHYSIOLOGY
07.011 - ELECTRORETINOGRAMS FULL FIELD BY LIGHT FLASHES IN COLOSSOMA MACROPOMUM
IN VIVO
PRISCILA RAFAELA LEÃO SOARES; ELTON HUGO LIMA DA SILVA SOUZA; ANDRÉ LUCAS CORRÊA DE
ANDRADE; THAMIRIS PINHEIRO SANTOS; STEPHANNIE CAROLINE BARROS LUCAS DA SILVA; FABRÍCIO
BEZERRA DE SÁ; PABYTON GONCALVES CADENA
UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO, RECIFE, PE, BRASIL.
Electroretinogram (ERG) is an electrophysiology method used to evaluate retinal activity,
especially the function of photoreceptors. The aim of this study was to develop a technique of
photopic ERG and flicker 30 Hz in Colossoma macropomum in vivo. The project was approved by
CEUA/UFRPE n. 140/2014. The ERG were recorded from 3 monopolar electrodes: Reference,
introduced in the dorsal subcutaneous area; Active DTL contact the center of the cornea; and
ground wire in the petri dish. Three stimuli of 3 cd/m2 at 5 Hz was made for the photopic record
and 20 stimuli of 3 cd/m2 with a frequency of 30 Hz for the flicker. The System used was
Neuropack 2 MEB-7102A/k of Nihon Kohdem. The exam consisted in eight fish anesthetized with
eugenol (30 mg/L), 4 in the morning (G1) and 4 in the afternoon (G2). The total average time was
7 minutes and 2 min for exam in 25 °C (water temperature). The means obtained in photopic
exam, implicit time and amplitude of the waves in the groups were: G1 (a 21.9 ± 2.66 ms and b
64.05 ± 22.85 ms) and (a 16.8 ± 3.14 µV and b 33.47 ± 10.71 µV); G2 (a 26.1 ± 3.44 ms and b 57.61
± 16.36 ms) and (a 15.22 ± 1.38 µV and b 27.57 ± 3.97 µV). For the flicker test results were
obtained the average of amplitude G1 (25.13 ± 19.22 µV) and G2 (12.31 ± 3.74 µV). For the implicit
time in G1 (33.71 ± 0.41 ms) and G2 (33.87 ± 0.42 ms), there was no significant difference between
groups. The application of the technique in fish in vivo, independent of time of day, show to be
viable in data acquisition and will contribute to future research.
07.012 - THYROXINE EFFECT AS ENDOCRINE DISRUPTOR IN BEHAVIOR OF TAMBAQUI
(COLOSSOMA MACROPOMUM)
THAMIRIS PINHEIRO SANTOS; ELYDA GRAZYELLE DA SILVA OLIVEIRA; PRISCILA RAFAELA LEÃO
SOARES; STEPHANNIE CAROLINE BARROS LUCAS DA SILVA; ANDRÉ LUCAS CORRÊA DE ANDRADE;
MARÍLIA CORDEIRO GALVÃO DA SILVA; MARILIA RIBEIRO SALES CADENA; PABYTON GONCALVES
CADENA
Endocrine disruptors chemicals (EDCs) are synthetic or natural substances that can affect the
endocrine system, causing severe effects on behavior, reproduction and functioning of
tissues/organs. Few studies have shown the thyroxine effect as an EDC in fish behavior. In this
framework, the objective of this study was to evaluate the toxic effects of thyroxine in behavior
and weight gain of tambaqui (Colossoma macropomum). The project was approved by CEUA-UFRPE
n. 096/2014. In aerated tanks at 25 °C,animals were divided into two groups, of 7 juveniles fish
40
each. The treatments were Control (C) and other exposed to thyroxine (T) at 10 ng/L for 90 days
in a chronic toxicity test. It was developed an ethogram by ad libitum method of movement
behaviors (MB) and behaviors associated with stress (BAS). Its application was realized by scan
sampling method. The MB, like Slow swimming (C 70.4% and T 63.4%) and Fast Swimming (C 8.2%
and T 3.8%) were not significantly different by Tukey test (p < 0.05). Therefore, in the BAS, the
Coprophagia and Eating Disorder (compulsive eating) food behaviors had a frequency of two and
three times higher in the T group compared to C group, respectively. After 90 days, the C group
showed higher weight gain (15.33 ± 2.81 g) compared to T group (11.43 ± 2.51 g). It was concluded
that the thyroxine increased the frequency of behaviors associated with stress and reduced weight
gain in the C. macropomum in the studied concentration.
07.013 - EFFECTS OF ESTRADIOL AND BISPHENOL AS ENDOCRINE DISRUPTORS ON THE
AGGRESSIVE BEHAVIOR OF BETTA SPLENDENS
ERICKA QUEIROZ DE SOUZA; ELYDA GRAZYELLE DA SILVA OLIVEIRA; ANDRÉ LUCAS CORRÊA DE
ANDRADE; STEPHANNIE CAROLINE BARROS LUCAS DA SILVA; THAMIRIS PINHEIRO SANTOS; PRISCILA
RAFAELA LEÃO SOARES; MARILIA RIBEIRO SALES CADENA; PABYTON GONCALVES CADENA
Hormones have their physiological effects, but can also induce toxic effects on aquatic biota. In
these cases, they are known as endocrine disruptor chemicals (EDC). Among them, estradiol (E2)
is the most common EDC present in the environment; additionally, bisphenol A (BPA) is a
composite from industrial sources. It also has estrogenic activity and persist in the environment.
In this way, the objective of this study was to evaluate the toxic effects of E2 and BPA as EDCs on
aggressive behavior and survival of Betta splendens. The project was approved by CEUA-UFRPE n.
017/2015. Fifteen male fish were placed in individual aquariums and divided in three groups:
control (Group C), estradiol (Group E) and bisphenol A (Group B), the last two exposed to a
concentration of 100 ng/L of the substance for 30 days. It was evaluated the behavior by scan
sampling method, opercular opening time (OOT) when the animal looked to the mirror and
survival. As a result, it was observed that only the Rest behavior had significantly different (p <
0.05) by Tukey test, between the group C (69.6 ± 20.1%) and groups E (45.3 ± 12.9%) and B (31.5
± 16.4%). There were no significant differences in OOT between groups C (126 ± 44 s), E (93 ± 48
s) and B (151 ± 40 s). Regarding survival, the animals of group E showed the double of mortality
compared to group B. It was conclude that E2 and BPA showed similar toxic effects in relation to
the behavior of B. splendens, but estradiol had higher mortality. This indicates the need to control
the discharge of these substances in the environment.
07.014 - SPREADING THE FUNCTION OF THE SYSTEMIC RNAI MACHINERY: REGULATION OF
STRESS RESISTANCE IN C. ELEGANS
HENRIQUE CAMARA; SILAS PINTO DA SILVA; RAFAEL FERRAZ CONCEICAO; EVANDRO ARAUJO DE
SOUZA; MARCELO A. MORI
UNIFESP/EPM, SAO PAULO, SP, BRASIL.
Introduction: The RNA interference (RNAi) pathway controls aging and stress resistance in
different species by mechanisms involving silencing by small RNAs (miRNAs and siRNAs). In C.
elegans, systemic spreading of exogenously administered double stranded RNA-mediated silencing
signals depends on the function of evolutionary conserved proteins, such as SIDs (Systemic RNA
Interference Defective) and RSDs (RNAi Spreading Defective). However, the physiological
functions of these proteins remain to be identified. One hypothesis is that they mediate intertissue
communication in response to environmental stress. Methods: We measured thermotolerance and
oxidative stress resistance of wild-type (N2) and sid-1, sid-2 or rsd-3 loss-of-function mutant
worms. For thermotolerance analysis, worms were heat stressed (28 oC) during their entire
lifespan, death events were counted and mean lifespan (ML - days) obtained. For the oxidative
stress protocol, worms were stressed with sodium arsenite (7.5mM or 10mM) for 8 hours at 20oC
and death events were counted. Data was compared using Log-rank test (p<0.05). Results: The
sid-1 and sid-2 mutations increased thermosensitivity of worms (MLsid-1: 4, n=68; MLsid-2: 5, n=113;
MLN2: 6; n=120). The sid-2 mutants were also more sensitive to oxidative stress while rsd-3 mutants
were more resistant (p<0.05). Conclusion: The RNAi spreading machinery is important to regulate
stress response in C. elegans. This is the first evidence for a physiological function of SIDs and
RSDs.
07.015 - INFLUENCE OF THE WATER BALANCE IN EMBRYONIC DEVELOPMENT IGUANA IGUANA
(REPTILIA, SQUAMATA)
LAURA BORELLI BORELLI THOMAZ CARREIRA; MARINA RINCON SARTORI; AUGUSTO SHINYA ABE
UNESP, RIO CLARO, SP, BRASIL.
Reptilian eggs have different levels of calcification, which interfere in the permeability of water
vapor. Water balance previous studies were carried out with rigid shells eggs. However, little is
known about the eggs of flexible shell that are most vulnerable. This work aimed to study the
water balance egg flexible shell Iguana iguana on 3 types of water potentials. This potentials were
determined from the following substrate incubation and water ratios: 1:1 dried 1:1.5
intermediary; 1:2 moist. During the embryonic period was determined the mass of eggs(MOE) and
embryos, the death rate of the treatments, the mass and size of hatchlings. The MOE at the end
of the incubation period on wet substrate (49 ± 1.11g) was 25% greater than the dry (39 ± 0.70 g)
and 7% higher than the intermediate (42 ± 0.98 g). The MOE varied between treatments from 50%
incubation. Embryos wet substrate and intermediate had higher masses than eggs in dry. The wet
substrate had the highest mortality rate of 40%, compared with 30% in the intermediate and 22%
in the dry. The hatchlings of wet substrate was 5% smaller than the offspring of the driest, both
at mass (12.44 ± 0.22 vs 13.17 ± 0.22) and in the rostrum caudal length (24.11 ± _ 0.39 vs. 25.39
± 0.39). Although eggs wet substrate increase more in mass due to increased water absorption,
the resulting offspring were not higher. Thus, the results are opposite to the hypothesis proposed
by previous studies that eggs incubated in wetter substrates tend to have higher newborns and
birth rate higher than the eggs incubated in drier.
THEME 08 - RESPIRATORY PHYSIOLOGY
08.031 - ANTAGONISM OF OREXIN RECEPTOR 1 (OX1) IN THE LOCUS COERULEUS (LC) DECREASES
THE HYPERCAPNIC VENTILATORY RESPONSE IN UNANESTHETIZED RATS IN WAKEFULNESS
DURING DARK PHASE
MARIANE CRISTINE VICENTE; LUCIANE HELENA GARGAGLIONI BATALHAO; MIRELA BARROS DIAS;
KÊNIA CARDOSO BÍCEGO
UNESP, JABOTICABAL, SP, BRASIL.
The orexins are involved in promoting and maintaining wakefulness and contribute to CO2/pH
chemoreception. LC is a chemoreceptor region that expresses an extensive population of orexin
receptor 1 (OX1R). Based on this, we tested the hypothesis that OX1R located in the LC participate
in the ventilatory response to hypercapnia and if this response is state-dependent. To this end,
we performed unilateral injections of SB-334867 (OX1R antagonist, 5 mM) into the LC of male
Wistar rats and evaluated the ventilatory response to 7% CO2 during wakefulness and sleep in the
dark phase. Hypercapnia induced an increase in ventilation in all groups compared to normocapnic
values. However, the antagonist injection (SB-334867) promoted an attenuation of the
hypercapnic chemorreflex during wakefulness (VE vehicle 15682 ± 109.6 mL.Kg-1.min-1 vs VE SB334867= 1.2475 ± 606 mL.Kg-1.min-1), but not during sleep (VE vehicle= 1366,0 ± 154,8 vs SB334687: 1396,5 ± 122,6 mL.Kg-1.min-1), due to changes in VT, in the dark phase. We suggest that
projections of orexin-containing neurons to the LC contribute, via OX1Rs, to the hypercapnic
chemoreflexduring wakefulness in the dark phase.
08.032 - CHRONIC ALLERGIC PULMONARY INFLAMMATION IS AGGRAVATED IN ANGIOTENSIN-(17) MAS RECEPTOR KNOCKOUT MICE
GISSELLE SANTOS MAGALHÃES; MARIA DA GLÓRIA RODRIGUES MACHADO; DAISY MOTTA-SANTOS;
ROBSON AUGUSTO SOUZA DOS SANTOS; LUCIOLA SILVA BARCELOS; MARIA JOSE CAMPAGNOLESANTOS
Angiotensin-(1-7)/Mas receptor axis is associated with attenuation of different inflammatory
processes. In this study, we investigated the involvement of Mas receptor in the inflammation and
airway remodeling in a model of chronic allergic pulmonary inflammation. FVBN-Mas KO control,
Mas KO sensitized with ovalbumin (OVA), WT control and WT sensitized with OVA. were used.
Mice were subjected to OVA sensitization (20 μg/mouse, ip, 4 times, 14 days apart) and OVA
challenge (1% nebulization/30 min, 3 times/week, for 4 weeks) to induce chronic allergic lung
inflammation. Mice were sacrificed 72h after the last nebulization. MasWT-OVA did not develop
inflammation or pulmonary remodeling, as it is observed with this protocol in BalbC mice.
However, MasKO-OVA showed a worse performance in the maximum load exercise test and
presented a significant increase in inflammatory cell infiltrate in the lung, collagen deposition in
the airways, increased thickening of the alveolar parenchyma, increased thickening of the smooth
muscle layer of the pulmonary arterioles and hypertrophy of the cardiomyocyte of the right
ventricle. Furthermore, MasKO-OVA showed an increase in ERK1/2 and SAPK/JNK phosphorylation
and an increase in pro-inflammatory cytokine (IL13) and chemokines (MCP-1/CCL2 and
RANTES/CCL5) in the lungs. These data indicate that genetic deletion of Mas receptor aggravates
lung inflammation and remodeling in mice subjected to a model of chronic asthma.
08.033 - VARIABLE VENTILATION IMPROVES RESPIRATORY FUNCTION BUT IMPAIRS RIGHT
VENTRICULAR PERFORMANCE IN ELASTASE-INDUCED EMPHYSEMA
CAIO GUILHERME RODRIGUES SANTOS WIERZCHON; ISABELA HENRIQUES LUCAS GUIMARÃES; GISELE
PADILHA; NAZARETH DE NOVAES ROCHA; RAQUEL SOUZA SANTOS; FERNANDA FERREIRA CRUZ;
PEDRO LEME SILVA; PATRICIA RIEKEN MACEDO ROCCO
Rationale: Chronic obstructive pulmonary disease (COPD) is a major global health issue. Intubation
and mechanical ventilation (MV) carry significant morbidity and mortality, and MV strategies in
COPD are controversial. We evaluated whether variable ventilation (VV) led to better lung and
cardiovascular functional effects than conventional ventilation (CV) in elastase-induced
emphysema.
Methods: 36 Wistar rats were randomly divided into 2 groups. In the emphysema (ELA) group, rats
received porcine pancreatic elastase 2 IU intratracheally once weekly for 4 weeks, whereas
controls (C) received saline. 5 weeks after last instillation, animals were ventilated with
VT=6ml/kg, PEEP=3 cmH2O and FiO2=0.4. Animals were randomly assigned to receive VV or CV.
VV was applied on a breath-to-breath basis as sequence of 600 randomly generated VTs (mean
VT=6ml/kg) with a 30% coefficient of variation. All animals were ventilated for 2h. Respiratory
mechanics, histology, and echocardiography were analyzed.
Results: VV decreased respiratory system elastance in C (p<0.001) and ELA (p<0.01). This was
followed by a reduction in volume-dependent portion of elastance (E2) in C (p<0.01) and ELA
(p<0.05). VV also reduced alveolar hyperinflation in the ELA group (p<0.05). However, right
ventricle area increased in ELA groups after VV in comparison to CV. CONCLUSION: Compared to
CV, VV improved lung morphofunctional parameters but increased right ventricular load.
08.034 - ACUTE RESPIRATORY DISTRESS SYNDROME DOES NOT AFFECT THE CARDIAC
IMPAIRMENT INDUCED BY OBESITY IN RATS
STEFANO DE AVELAR ALCHORNE TRIVELIN; CYNTHIA SANTOS SAMARY; MILENA VASCONCELLOS DE
OLIVEIRA; NAZARETH DE NOVAES ROCHA; PEDRO LEME SILVA; PATRICIA RIEKEN MACEDO ROCCO;
LIGIA DE ALBUQUERQUE MAIA
Rationale: Obesity is a serious public health issue worldwide. Obese individuals seem more
susceptible to respiratory infections. However, the pathophysiological mechanisms of acute
respiratory distress syndrome (ARDS) in the presence of obesity are unclear. We sought to
characterize the effects of ARDS in a rat model of obesity.
Methods: Obesity was induced by early postnatal overfeeding in Wistar rats. For this purpose, we
reduced litter size to 3 pups/litter (Ob, n=18). In controls (C, n=18), a normal litter size (10
pups/litter) was used. Body weight was monitored every 7 days. On postnatal day (PN) 120, an
oral glucose tolerance test was performed. On PN150, both C and Ob groups were treated with
saline (SAL) or Escherichia coli lipopolysaccharide (LPS) intratracheally. After 24h, animals were
anesthetized, examined by echocardiography, and euthanized by cardiac puncture.
Results: Ob-SAL rats had greater body weight, visceral fat mass and fasting hyperglycemia at
adulthood than C-SAL. Additionally, Ob-SAL animals had lower left ventricle diameter but a larger
interventricular septum, posterior wall thickness and isovolumic relaxation time when compared
to C-SAL. Ob-ARDS rats had a larger interventricular septum, posterior wall thickness and lower
PAT/PET ratio compared to C-ARDS.
Conclusion: This model of obesity induced by early postnatal overfeeding led to changes in heart
function and morphology, but no further cardiac impairment was observed after ARDS induction.
08.035 - MULTIPLE DOSES OF ADIPOSE TISSUE-DERIVED MESENCHYMAL STROMAL CELLS YIELD
BENEFICIAL EFFECTS WITH IMMUNOSUPPRESSION IN EXPERIMENTAL ASTHMA
LIGIA LINS DE CASTRO; JAMIL ZOLA KITOKO; DANIELY DAS GRAÇAS ROBERTO; LUÍSA ALCHAAR DE
FARIA; SIDNEY TEIXEIRA JUNIOR; SORAIA CARVALHO ABREU; DEBORA GONÇALVES XISTO; PRISCILLA
CHRISTINA OLSEN; MARCELO MARCOS MORALES; PATRICIA RIEKEN MACEDO ROCCO
Rationale: Glucocorticoids are widely used in asthma, but may cause immunosuppression.
Mesenchymal stromal cells (MSCs) may be an alternative due to their immunomodulatory
properties; but, no study has evaluated whether multiple doses of MSCs may be
immunosuppressive. We evaluated the effect of multiple doses of human adipose tissue-derived
MSCs (AD-MSC) in a model of asthma induced by house dust mites (HDM).
Methods: 48 C57BL/6 mice were intranasally challenged with saline (CTRL) or HDM. Both groups
then received daily intravenous saline (SAL), AD-MSC (1x106) or dexamethasone (DEXA, 1mg/kg)
24h after last challenge for 3 days. 7 days after the last challenge, collagen fiber content, and
total and differential cell counts in bronchoalveolar lavage fluid (BALF), thymus, bone marrow,
and mediastinal lymph nodes were analyzed.
Results: HDM increased collagen fiber content, which was similarly reduced by DEXA and AD-MSC.
Total leukocyte and eosinophil counts in all tissues were increased in HDM vs. CTRL. Both DEXA
and AD-MSCs reduced total leukocytes and eosinophils in BALF, thymus and lymph nodes, but only
AD-MSCs did so in bone marrow. HDM increased Treg in BALF, an effect countered by DEXA. In the
thymus, DEXA increased Treg and reduced thymus weight compared to the other groups. AD-MSCs
did not affect Treg.
Conclusion: Multiple doses of AD-MSCs had beneficial effects on lung remodeling, however with
immunosuppression.
08.036 - BIOLOGICAL IMPACT OF DIFFERENT VENTILATOR STRATEGIES DURING OPEN
ABDOMINAL SURGERY IN RATS
SIDNEY TEIXEIRA JUNIOR1; CYNTHIA SANTOS SAMARY1; MILENA VASCONCELLOS DE OLIVEIRA1;
CÍNTIA LOURENÇO SANTOS1; ROBERT HUHLE2; MARCELO GAMA DE ABREU2; PAOLO PELOSI3; PEDRO
LEME SILVA1; PATRICIA RIEKEN MACEDO ROCCO1; LIGIA DE ALBUQUERQUE MAIA1
1.UFRJ, RIO DE JANEIRO, RJ, BRASIL; 2.DRESDEN UNIVERSITY OF TECHNOLOGY, DRESDEN,
ALEMANHA; 3.UNIVERSITY OF GENOA, GENOA, ITALIA.
Rationale: About 234 million major surgical procedures are performed worldwide every year. We
sought to analyze respiratory function and the biological impact of different ventilatory strategies
during open abdominal surgery in rats.
Methods: 28 healthy Wistar rats (394.1±59.9g) were anesthetized, tracheotomized, paralyzed and
mechanically ventilated for 4 hours. After baseline data collection, laparotomy with bowel
manipulation was performed. Animals were then randomly allocated across 4 groups (n=7/group):
1) moderate PEEP (3 cmH2O), low VT (7mL/kg) and RM every 1 hour; 2) high PEEP (6 cmH2O), low
VT (7mL/kg) and RMs at the start and end of the experiment; 3) low PEEP (1 cmH2O), low VT
(7mL/kg) without RMs; 4) low PEEP (1 cmH2O), high VT (14 mL/kg) without RMs.
Results: Oxygenation improved in all animals regardless of ventilator strategy. Respiratory system
and lung elastance, peak airway and transpulmonary pressures were lower in groups 1 and 2 vs.
groups 3 and 4. IL-6 mRNA expression was increased in all groups regardless of ventilator strategy.
Group 3 exhibited reduced amphiregulin expression, and group 4 exhibited increased PCIII mRNA
expression.
41
Conclusion: In this model of abdominal surgery, RMs associated with moderate or higher PEEP
levels improved lung function, but increased amphiregulin mRNA expression, suggesting
ventilator-induced lung injury. Hence, mechanical ventilation with low VT and PEEP seems to be
the best ventilator strategy during open abdominal surgery.
08.037 - BONE MARROW-DERIVED VERSUS ADIPOSE TISSUE-DERIVED MESENCHYMAL STROMAL
CELLS IN EXPERIMENTAL ALLERGIC ASTHMA INDUCED BY HOUSE DUST MITES
JAMIL ZOLA KITOKO; LIGIA LINS DE CASTRO; ANA PAULA NASCIMENTO; TAINÁ BATISTA DE OLIVEIRA;
SORAIA CARVALHO ABREU; DEBORA GONÇALVES XISTO; MARCELO MARCOS MORALES; PATRICIA
RIEKEN MACEDO ROCCO; PRISCILLA CHRISTINA OLSEN
Rationale: Mesenchymal stromal cells (MSCs) may be an excellent alternative for asthma therapy
due to their immunomodulatory properties. Most experimental studies of MSC effects have been
performed in experimental asthma models induced by innocuous allergens, such as ovalbumin,
and therapy administered during or before asthma induction. The aim of this translational study
was to evaluate the effects of MSC therapy on established house dust mite-induced asthma.
Methods: C57BL/6 mice were intranasally challenged multiple times with house dust mites (HDM)
and treated with bone-marrow (BM) or adipose tissue (AD)-derived MSCs (1x105 intratracheally)
24h after the last challenge. Analyses were performed 7 days after MSC therapy.
Results: In bronchoalveolar lavage fluid, AD-MSCs (3.35±0.37, n=7), but not BM-MSCs, reduced
increases in leukocyte levels, including eosinophils and CD4+ T cells, generated by HDM exposure
(4.81±0.68, n=6). Neither AD-MSCs nor BM-MSCs impacted peribronchiolar inflammatory pockets
observed in challenged mice. No differences in lung fibrosis were observed among groups.
Conclusion: Despite promising findings about MSC pretreatment in experimental asthma, our
results using a more clinically relevant allergen suggest that, once inflammation and remodeling
are already established, cell therapy yields little improvement of lung inflammation and does not
affect remodeling.
08.038 - MATURATIONAL CHANGES IN THE BÖTZINGER COMPLEX POST-INSPIRATORY NEURONS
OF RATS
DAVI JA MORAES; MELINA PIRES DA SILVA; WAMBERTO ANTONIO VARANDA; BENEDITO HONORIO
MACHADO
FMRP/USP, RIBEIRAO PRETO, SP, BRASIL.
Bötzinger Complex (BötC) post-inspiratory (post-I) neurons are part of ventral respiratory group
and are involved in the control of respiratory phase duration and upper airway resistance. The
post-I neurons are immature at birth and post-natal developmental changes in its
electrophysiological properties have yet to be investigated. We investigated the respiratory
pattern, the electrophysiological properties and phenotype of BötC post-I neurons on different
post-natal days [neonatal, P1-5 (n=7); intermediate, P6-15 (n=19); juvenile, P15-21 (n=29); adult,
P22-40 (n=35)] in the in situ preparation of rats. Age-dependent increase in both duration of
expiration and post-inspiration over the first 14 days of life was observed. Similarly, the post-I
increase in the upper airway resistance also changed from a small with a short duration to a
pattern of sharp rise and longer duration (> 14 days). Regarding the glicinergic and glutamatergic
BötC post-I neurons, burst activity, low threshold spike and low-voltage calcium current increased
with age. Changes in subthreshold responses were also observed, including an increase in input
resistance and excitability. Rheobase current, spike frequency adaptation and action potential
duration decreased progressively during postnatal development. We conclude that postnatal
maturation of the electrophysiological properties of BötC post-I neurons may explain the
development changes in the respiratory pattern and respiratory control of upper airway resistance
in rats.
08.039 - FAILURE IN CENTRAL RESPIRATORY 5HT-DEPENDENT CHEMORECEPTION IN A GENETIC
MODEL OF EPILEPSY: POTENTIAL IMPACT FOR SUDEP
LEONARDO TEDESCO TOTOLA; ANA CAROLINA T TAKAKURA; JOSE ANTONIO OLIVEIRA; NORBERTO
GARCIA-CAIRASCO; THIAGO S MOREIRA
Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death in patients with
refractory epilepsy. There is still a debate in the literature if the respiratory arrest is the primary
cause of death. Respiratory chemoreceptors neurons located in retrotrapezoid nucleus (RTN) are
one of the main groups responsible for controlling breathing automaticity and receives a dense
serotonergic innervation. Here, we ask whether the destruction of a type of chemoreceptor
neurons that expresses the transcription factor Phox2b and is non-catecholaminergic
(Phox2b+/TH-) could affect breathing in a rat model of tonic-clonic seizures, with susceptibility
to audiogenic seizures (the Wistar Audiogenic Rat (WAR) strain (WAR: 340-496 g, n = 6-7; CEUA:
108/2014). The number of Phox2b+/TH- neurons in the RTN was reduced (79±11%) in WAR. The
WAR have reduced resting ventilation (VE) by 35 ±10% and the increase in VE elicited by
hypercapnia (7% CO2) by 51 ±7%. Furthermore, we also showed that the VE response to serotonin
(1 mM - 50 nl) into the RTN region was significantly reduced in WAR due to the reduction in the
number of 5-HT varicosities within the marginal layer of the RTN region. Our results suggest a
respiratory disorder, as well as a reduction of the serotonergic neurotransmission within the RTN
region which justify to further use WAR as a suitable model to study increased risk factors and the
mechanisms associated with SUDEP.
08.040 - EFFECTS OF TWO DOSES OF BONE MARROW-DERIVED MESENCHYMAL STROMAL CELLS
IN A MODEL OF ELASTASE-INDUCED SEVERE EMPHYSEMA
HANANDA ALYNA POGGIO; FERNANDA FERREIRA CRUZ; MARIANA ALVES ANTUNES; JAMIL ZOLA
KITOKO; NAZARETH DE NOVAES ROCHA; PRISCILLA CHRISTINA OLSEN; PATRICIA RIEKEN MACEDO
ROCCO; MARCELO MARCOS MORALES
Rationale: Emphysema is characterized by the enlargement of air spaces. accompanied by
parenchymal destruction and impaired pulmonary regeneration. A number of preclinical studies
have shown that a single dose of bone marrow (BM)-derived mesenchymal stromal cells (MSCs)
attenuates lung inflammation and apoptosis in experimental emphysema, with controversial
results. We hypothesized that, in a model of elastase-induced severe emphysema, the beneficial
effects of BM-MSC therapy would depend on the number of doses.
Methods: Forty female C57BL/6 mice were randomly assigned into 2 groups. In the emphysema
(E) group, mice received 0.2IU of porcine pancreatic elastase (PPE) once week for 4 weeks,
whereas controls (C) received intratracheal saline (SAL) using the same protocol. One day after
the last administration of SAL or PPE, two doses of SAL or MSCs (2x106) were intratracheally
instilled with a 1-week interval between doses.
Results: One week after the last treatment, E-MSC animals exhibited no improvement on static
elastance, but reduced mean linear intercept IN 19,4%, lung hyperinflation and collapsed alveoli
were reduced in 34%, elastolysis reduction in E-MSC group was of 10% when compared to E group.
Airway fibrosis was 37,26% lower in the E-MSC than in E-SAL animals.
Conclusion: In the current model of elastase-induced severe emphysema, two doses of MSCs
yielded reduced lung inflammation, fibrosis and elastolysis, resulting in pulmonary improvement.
Supported by: FAPERJ, CNPq, CAPES, DECIT/MS
08.041 - AVALIAÇÃO DA INFLUÊNCIA DO CORPO CAROTÍDEO E DA ATIVIDADE NEURONAL NO NTS
SOBRE ALTERAÇÔES VENTILATÓRIAS INDUZIDAS PELA DESNUTRIÇÃO PROTEICA PERINATAL
IALLY FABIANE DA SILVA1; VIVIANE DE OLIVEIRA NOGUEIRA2; GLAUBER DOS SANTOS FERREIRA DA
SILVA3; WALDECY DE LUCCA JÚNIOR4; LARISSA RESENDE OLIVEIRA4; JOSÉ LUIZ DE BRITO ALVES 2;
JOÃO HENRIQUE DA COSTA SILVA2
1.UNIVERSIDADE FEDERAL DE PERNAMBUCO-CENTRO ACADÊMICO DE VITÓRIA, PASSIRA, PE,
BRASIL; 2.UNIVERSIDADE FEDERAL DE PERNAMBUCO, VITÓRIA DE SANTO ANTÃO, PE, BRASIL;
3.FACULDADE DE CIÊNCIAS AGRÁRIAS E VETERINÁRIAS-UNESP, SÃO PAULO, PE, BRASIL;
4.UNIVERSIDADE FEDERAL DE SERGIPE, ARACAJU, SE, BRASIL.
Ratos submetidos à desnutrição proteica perinatal aumentam o ritmo respiratório e a
quimiossensibilidade ao CO2. Porém, pouco se sabe sobre o papel do corpo carotídeo (CB) e
atividade neuronal no núcleo do trato solitário (NTS). Utilizou-se ratos Wistar (30 dias) nascidos
de mães que receberam dieta normoproteica (17% proteína,NP) ou hipoproteica (8% proteína,HP)
perinatal dos quais removeu-se o CB (CBD) ou não (SHAM). Após 24h, aferiu-se pressão arterial
(PA), frequências cardíaca (FC) e respiratória (FR), antes e após 7% de CO 2. Por imunofluorescência
de FOS estudou-se a atividade neuronal do NTS. Os níveis basais de PA e FC foram análogos entres
os grupos. Após 7% de CO2, a resposta na PA foi similar entre NP e HP_SHAM, e foi maior nos NP e
HP_CBD, sem variação na FC entre os grupos. FR basal do HP_SHAM foi maior que NP_SHAM
(NP_SHAM:115±2 vs. HP_SHAM:134±2). Diferença abolida após CBD (NP_CBD:114±2 vs.
HP_CBD:125±2). A exposição ao CO2 causa aumento no grupo HP (NP_SHAM:166±3;
HP_SHAM:181±3), abolida após CBD (NP_CBD: 157±3; HP_CBD:165±6 resp.min-1). Expressão de FOS
no NTS intermediário e rostral foi análoga entre os grupos. No NTS caudal, HP_SHAM teve menor
expressão que NP_SHAM (NP_SHAM: 12±1, HP_SHAM:8±1; NP_CBD:5±1; HP_CBD:4±1 células FOS+).
O aumento no ritmo respiratório e maior sensibilidade ao CO 2 induzidos por desnutrição perinatal
parecem associar-se a integridade do CB, mas não a atividade neuronal no NTS.
08.042 - AVALIAÇÃO DOS PARÂMETROS VENTILATÓRIOS DE RATOS NEONATOS PROVENIENTES
DE MÃES SUBMETIDAS À DESNUTRIÇÃO PROTEICA
KASSIA LETICIA CHAGAS SOUZA COUTINHO1; LUANA DARC SILVA DE ANDRADE1; VIVIANE DE OLIVEIRA
NOGUEIRA1; MONIQUE ASSIS DE VASCONCELOS BARROS1; GLAUBER DOS SANTOS FERREIRA DA
SILVA1; JOSÉ LUIZ DE BRITO ALVES2; JOÃO HENRIQUE DA COSTA SILVA1
1.UNIVERSIDADE FEDERAL DE PERNAMBUCO, VITORIA DE SANTO ANTÃO, PE, BRASIL; 2.FACULDADE
DE CIÊNCIAS AGRÁRIAS E VETERINÁRIAS-UNESP, SÃO PAULO, SP, BRASIL.
Introdução: Estudos sugerem que a desnutrição proteica perinatal promove alterações no ritmo
respiratório basal e na quimiossensibilidade respiratória. Contudo, é incerto em qual momento da
vida iniciam-se tais alterações. Neste sentindo, avaliou-se a atividade respiratória de ratos
neonatos provenientes de mães submetidas à desnutrição. Métodos: Foram utilizados ratos Wistar
provenientes de mães que receberam no período perinatal dieta normoproteica (NP, 17% de
proteína) ou hipoproteica (HP, 8% de proteína). Ao 1º, 7º, 14º, 21º dia de vida, por pletismografia,
mensurou-se a frequência respiratória (FR), volume corrente (VT) e ventilação (VE). Os dados
estão expressos como média±EPM, e foi utilizado teste t-Student não pareado e p<0,05. Todos os
processos aprovados por CEUA (2014-48). Resultados: No 1° dia de vida, houve redução no VT do
grupo HP (NP: 5,8±0,3 vs. HP: 4,5±0,3 mL.Kg-1), mas sem alteração na FR e VE. No 7° dia, o grupo
HP apresentou redução no FR (NP: 237,4±7,0 vs. HP: 178,3±17,1 resp.min-1, n=4 vs. n=6), sem
alteração no VT e VE. No 14° dia de vida, foi observado aumento nos valores de VT (NP: 7,9±0,9
vs. HP: 15,3±2,0 mL.Kg-1) e VE (NP: 1313±56,5 vs. HP: 2420±279,7 mL.Kg-1.min-1), mas sem
alteração de FR. No 21° dia de vida, nenhuma diferença foi observada FR, VT e VE. Conclusão:
Esses dados sugerem que a desnutrição proteica perinatal deve alterar a maturação da rede
respiratória em ratos neonatos, afetando o padrão ventilatório destes animais.
08.043 - BLOOD VARIABLES AND VENTILATORY EVALUATION OF THE OFFSPRING OF RATS
SUBJECTED TO MATERNAL MALNUTRITION
VIVIANE DE OLIVEIRA NOGUEIRA1; JOSÉ LUIZ DE BRITO ALVES 1; DANILO AUGUSTO FERREIRA
FONTES1; GLAUBER DOS SANTOS FERREIRA DA SILVA2; JOÃO HENRIQUE DA COSTA SILVA1
1.UFPE, RECIFE, PE, BRASIL; 2.UNESP, JABOTICABAL, SP, BRASIL.
Perinatal protein malnutrition promotes increased respiratory rate and sensibility to CO2 in male
offspring. In order to evaluate the role played by carotid body and blood variables. Juvenile Wistar
rats (30-d-old) from dams undergoing normal protein (17% protein, NP) or low protein (8% protein,
HP) diet in the perinatal period were subjected to removal (CBD) or not (SHAM). Arterial PO2,
PCO2, blood pH, respiratory frequency (FR) and ventilation (VE) were evaluated before and during
exposure to hypercapnic mixture (7% CO2). Data are expressed as mean±SEM, we used the Student
t-test or two-way ANOVA and Bonferroni post test, considering p<0.05. NP and HP rats exhibited
similar levels to gasometric PO2 (NP: 111 ± 5 vs. HP: 105 ± 7 mmHg n=7 vs n=11), PCO2 (NP: 29 ±
1 vs. HP: 28 ± 1 mmHg) and pH (NP: 7.42 ± 0.02 vs HP: 7.44 ± 0.01). Moreover, HP-SHAM exhibited
higher than the baseline FR compared NP-SHAM (NP-SHAM: 113 ± 4 vs. HP-SHAM: 132 ± 6 resp/min,
n=27 vs. n=32). However, this difference was abolished after CBD (NP-CBD: 114 ± 1 vs HP-CBD:
125 ± 2 resp/min, n=22 vs. n=14). Moreover, we found no changes in VE. After exposure to CO2,
HP-SHAM group had higher FR in relation to NP-SHAM, which was abolished after CBD (NP-SHAM:
161 ± 3, HP-SHAM: 179 ± 6, NP-CBD: 159 ± 3, HP-CBD: 165 ± 6 resp/min) and no changes it was
observed in VE. Increased respiratory rate and sensibility to CO2 observed in malnourished rats
were not associated with changes in arterial blood gas and seems to be dependent on the integrity
of the peripheral carotid bodies.
08.044 - MAGNETIC TARGETING OF MESENCHYMAL STROMAL CELLS IN A MURINE MODEL OF
SILICOSIS
LUISA HELENA ANDRADE DA SILVA1; EMILIA CELMA DE OLIVEIRA LIMA2; FERNANDA FERREIRA CRUZ1;
PATRICIA RIEKEN MACEDO ROCCO1
1.UNIVERSIDADE FEDERAL DO RIO DE JANEIRO, RIO DE JANEIRO, RJ, BRASIL; 2.UNIVERSIDADE
FEDERAL DE GOIÁS, GOIÂNIA, GO, BRASIL.
We aimed to enhance Mesenchymal Stromal Cells (MC) lung engraftment, by magnetic targeting,
increasing their beneficial effects on a murine model of silicosis. For this purpose, C57Bl6 mice
were intratracheally instilled with saline or silica particles. After 14 days, MC were incubated with
iron oxide nanoparticles (80μg/ml) for 24 hours, becoming magnetically responsive. Then, the
animals were treated with saline (S) or 105 MC and half of them, randomly, received external
neodymium magnets (MAG) around the thorax. For each experimental group, three animals were
used. 48 hours later, animals were harvested. Mice treated with MC and external magnets had
more iron in their lungs, suggesting more MC retention (S: 34.3 ± 5.1; MC: 62.7±5.4; MC-MAG:
81.5±1.5 µg/g). In these animals, the treatment also reduced granuloma fraction area (S:
97.3±5,1; MC: 62.7±3,4; MC-MAG: 58.5±6.5 %), alveolar thickening and cellularity (S: 35.2± 3.1;
MC: 27.9±3.4; MC-MAG: 25.5±4.1 %). Lastly, MC-MAG treatment also reduced static elastance (C:
27,9±1,5; S: 37,9±10,6; MC: 33,6±2,7; MC-MAG: 28,4±0,5 cmH2O.mL-1). These data demonstrate
that magnetic targeting of MC to lungs is a promising alternative and future trials will be
conducted in order to evaluate long-term effects and cellular viability.
Silva, L.H.A; Lima, E.C.O.; Cruz, F.F.; Rocco, P.R.M.
08.045 - SGLT1 PROTEIN EXPRESSION IN ALVEOLAR CELLS OF LUNG: POTENTIAL INVOLVEMENT
WITH REDUCTION OF GLUCOSE CONCENTRATION OF AIRWAY SURFACE LIQUID IN DIABETIC RATS
TALES LYRA DE OLIVEIRA1; NÁVYLLA CANDEIA DE MEDEIROS1; POLLIANE MARIA CAVALCANTEARAÚJO1; IGOR SANTANA DE MELO1; ROBINSON SABINO-SILVA2
1.UNIVERSIDADE FEDERAL DE ALAGOAS - UFAL, MACEIÓ, AL, BRASIL; 2.UNIVERSIDADE FEDERAL DE
UBERLÂNDIA - UFU, UBERLÂNDIA, MG, BRASIL.
The hyperglycemia promotes an increase of the glucose concentration in the airway surface liquid
(ASL) which facilitates the growth of microorganisms and respiratory infections. This study aimed
to evaluate the effect of diabetes and isoproterenol treatment (ISO) on the glucose and water
fluxes in lung via glucose transporters (SGLT). The diabetes was induced by alloxan (40 mg/kg, iv)
21 days before the study. On 22nd day, the non-diabetic and diabetic rats were treated
intranasally with saline, isoproterenol (5 mg/kg) or phlorizin (SGLT inhibitor; 10-3 M) and after 2h
were performed the collect of the bronchoalveolar lavage and lung removal. Thus, was realized
the measurement of volume and glucose concentration of ASL and SGLT1 immunohistochemistry
analyses of alveolar epithelium. ANOVA/Newman-Keuls test (p < 0.05). The volume of ASL and ASL
glucose concentration was increased (p < 0.05) in diabetics rats. The ISO promoted the SGLT1
translocation to the plasma membrane of pneumocytes and reduces (p < 0.05) 17% and 50% the
volume and the glucose concentration in ASL, respectively. The phlorizin produces opposite effect
compared with the observed in ISO. The present findings show that phlorizin- and isoproterenolinduced changes correlate with SGLT1 expression in plasma membrane of pneumocytes, which
can participate in the lung dysfunctions reported by diabetic patients.
08.046 - DEPLETION OF THE BULBOSPINAL CATECHOLAMINERGIC NEURONS REDUCES THE
HYPOXIC VENTILATORY RESPONSE IN UNANESTHETIZED RATS
MILENE RODRIGUES MALHEIROS; ANA CAROLINA T TAKAKURA; THIAGO S MOREIRA
42
The catecholaminergic (C1) neurons that reside in the rostral half of the ventrolateral medulla
regulate blood pressure, the CRF/ACTH/corticosterone cascade, autonomic glucoprivic responses
and breathing. Although pharmacological evidence suggests that C1 neurons regulate breathing,
a specific contribution to respiratory control has not been demonstrated, particularly in vivo.
Here, we evaluate the role of the bulbospinal C1 cells in the control of breathing under hypoxia
(8% O2) or hypercapnia (7% CO2) condition in unanesthetized rats. Bilateral injection of the
immunotoxin anti-dopamine β-hydroxylase-saporin (anti-DβH SAP) was done in adult male Wistar
rats (250-270g, N = 5-6/group, CEUA: 07/2014). Bilateral injections of anti-DβH SAP (2.1 ng/100
nl) into spinal cord selective destroy the bulbospinal C1 neurons, but spare the serotonergic
neurons in the same region. Depletion of the C1 cells induced by bilateral injection of anti-DβHSAP into the spinal cord reduced the increase in respiratory frequency (fR) (121 ± 10, vs. saline
140 ± 12 bpm), minute ventilation (VE) (330 ± 29, vs. saline: 362 ± 36 ml/min), but did not change
tidal volume (VT) (2.6 ± 0.3, vs. saline: 2.7 ± 0.2 ml) elicited by hypoxia. The respiratory changes
evoked by hypercapnia challenge were not altered after depletion of bulbospinal C1 cells. The
present study indicates that bulbospinal C1 neurons could be involved in the regulation of
breathing and provide evidence that they may contribute to the hypoxic ventilatory response.
08.047 - EFFECTS OF CARBACHOL MICROINJECTION IN THE COMMISSURAL NUCLEUS OF THE
SOLITARY TRACT ON SYMPATHETIC AND RESPIRATORY ACTIVITIES
WERNER ISSAO FURUYA
UNESP, SÃO CARLOS, SP, BRASIL.
Previous data from our laboratory have shown that microinjection of acetylcholine in the
commissural nucleus of the solitary tract (cNTS) increases phrenic nerve (PN) burst frequency and
change the coupling pattern between thoracic sympathetic (tSN) and PN activities. In the present
study we investigated the contribution of the muscarinic receptors in the sympathetic and
respiratory changes elicited by cholinergic activation in the cNTS. Decorticated arterially-perfused
in situ preparations of juvenile male Holtzman rats (60-90 g, n=20) were obtained and used to
evaluate the changes in tSN, PN, hypoglossal (HN) and cervical vagus nerve (cVN) activities elicited
by microinjections of carbachol (muscarinic agonist, 5 mM, 60 nL) in the cNTS. Carbachol
increased tSN activity (19±10 vs vehicle 1±3%, p<0.05) and the PN burst amplitude (24±5 vs vehicle
-1±1%, p<0.05), however did not change the PN frequency (13±10 vs vehicle -5±2%, p>0.05).
Carbachol also increased the amplitude (46±23 vs vehicle -4±3%, p<0.05) and the pre-I duration of
HN (386±62 vs vehicle 110±40 ms, p<0.05), and also increased both insp and post-insp components
of cVN activity (41±13 vs vehicle -1±2%, p<0.05). Our data suggest that the effects of carbachol
on respiratory and sympathetic activities may indicate that muscarinic receptors are expressed in
cNTS neurons that send projections to distinct pontine-medullary nuclei involved in the control of
sympatho-respiratory activity.
THEME 09 - ENDOCRINE & METABOLISM
09.040 - ANGIOTENSIN-(1-7) ICV MICROINJECTION INCREASES WATER INTAKE AFTER WATER
DEPRIVATION
RAONI DA CONCEICAO DOS-SANTOS; BRUNO PAES LEME FERREIRA; LÍVIA DA ROCHA NATALINO
MONTEIRO; ANDRÉ DE SOUZA MECAWI; LUIS CARLOS REIS
UFRRJ, SEROPÉDICA, RJ, BRASIL.
Several neuroendocrine mechanisms respond to small alterations of blood osmolarity influencing
water and sodium excretion and/or intake. Water deprivation (WD), induces dehydration, which
is compensated by several mechanisms, such as thirst. The classic angiotensinergic system
modulates those responses. ANG-(1-7) often counterbalances the angiotensin-II actions. However,
little information is available regarding this peptide and hydroelectrolytic regulation.
Anesthetized male Wistar rats were submitted to stereotaxic surgery for cannula placement on
the lateral ventricle. The animals were adapted to metabolic cages with free access to water,
hypertonic saline (1.8%) and food. The protocol consisted of ICV injection of ANG-(1-7) (3nmol in
2μl) or vehicle (0.9% NaCl), in either normohydrated (control - CT) or 24 hours WD animals.
In basal condition ANG-(1-7) does not affect water and sodium intake. The WD-vehicle animals
ingested more water (25 ± 4,7 vs 5,9 ± 2,7 ml, p<0.05 at 240 min) and saline (9 ± 2,4 vs 1 ± 1 ml,
p<0.05 at 120 min) than CT-vehicle, while the WD-ANG showed a faster increase in water (29,1 ±
4,3 vs 5,1 ± 2,3 ml, p<0.05 at 60 min) and non-significant increase in saline (7,1 ± 2,4 vs 1,6 ± 1,6
ml, p>0.05 at 300 min) compared to CT-vehicle.
The marked increases in water intake after ANG-(1-7) microinjection, indicate that it is possibly
involved in the responses to WD. Facilitating water intake and blunting NaCL intake in order to
recover homeostatic values of osmolarity.
09.041 - ETHANOL ACUTE EXPOSITION DURING PREGNANCY: DAMAGE ON SENSORY-MOTOR AND
REPRODUCTIVE DEVELOPMENTOF FEMALE OFFSPRING
MONALIZA LOPES DOS SANTOS; JORDANA ANDRADE DOS SANTOS; ADRYANO AUGUSTTO VALLADÃO
DE CARVALHO; CAIO CESAR BARBOSA; THAMYRES CUNHA RODRIGUES; RENATA KARINE DE
CARVALHO; RENATA MAZARO- COSTA
Ethanol (EtOH) prenatal exposure induces morphological and behavioral effects, so the aim of this
study was to evaluate the sensory-motor and the reproductive developments of F1 female rats
obtained from mothers acutely exposed to EtOH during pregnancy. Wistar female rats were used,
weighing 240 g, on the 14th gestational day were weighed and distributed into groups: Control
(CTRL) and EtOH that received the dose 3g/kg ip 20%. The pups obtained from CTRL and EtOH
groups were evaluated by sensory-motor parameters: ear, eye and vaginal opening, eruption of
incisors, holding reflex, postural reflex, negative geotaxis, depth avoidance, free fall, auditory
startle, whiskers location and balance on edge. During adult phase estrouscycle and sexual
behavior were evaluated (CTRL n=10; EtOH n=9). The results were analyzed by t test, p≤0.05 as
significant. In EtOH F1 the sensory motor development showed acceleration: eruption of incisors
(p=0.04), ear (p<0.0001) and eye (p=0.04)opening; and delayed on depth avoidance reflex
(p=0.04) and whiskers location (p=0.003) compared to the CTRL. The latency of the estrous cycle
was similar among groups (11/2 cycle in 7 days), but there was an elongation of estrus phase in
EtOH (p=0.04) and condensed diestrus 1 phase (p=0.04). However, sexual behavior showed no
statistical difference, despite 30% increase of lordosis. These results showed acceleration of
sensory development and delay of motor and sexual parameters from EtOH F1
09.042 - EFFECT OF ANGIOTENSIN-(1-7) ON OVULATION IN GOATS SUBJECTED TO TAI
PROTOCOL
AMILTON PAULO RAPOSO COSTA; ANDRÉIA DA SILVA COSTA; JOSÉ ADALMIR TORRES DE SOUZA;
FELIPE PEREIRA DA SILVA BARÇANTE; ANTÔNIO DE SOUSA JÚNIOR; JOILSON FERREIRA BATISTA;
ÍCARO OLIVEIRA TORRES DE SOUZA
UNIVERSIDADE FEDERAL DO PIAUI, TERESINA, PI, BRASIL.
Previous study showed an increase in estradiol production near ovulation in sheep subjected to
estrus synchronization when applying inhibitor of the ACE. This response may be due to the
increase of angiotensin-(1-7) (ang-(1-7). In this study was evaluated the effects of ang-(1-7) in
ovulation rate of goats subjected to TAI protocol. All animals received 60mg of
medroxyprogesterone acetate in intravaginal sponges for 11 days. On the 9th day received im,
eCG 300UI and cloprostenol 125μg. On 11th day the sponges were removed and on 12 and 13th
days the animals received subcutaneously: control group 30μg/kg of cyclodextrin diluted in 2ml
of distilled water and the angiotensin group received association ang-(1-7)+cyclodextrin,
corresponding to 20μg/kg of ang-(1-7). The AI was performed 38 and 50 hours after sponge removal
with frozen semen. Seven days after the last AI were collected blood from the jugular vein of 20
animals angiotensin group and 19 in the control. The serum was frozen at -20°C until analysis for
ELISA test. It was considered that there was ovulation when P4 concentration was above 1ng/ml.
The results showed that the control group presented 73.68% of ovulation and angiotensin 80% and
there was no significant difference (P=0.72), according to Fisher's exact test at 5% probability
between the two treatments. The application of ang-(1-7) in the dose of the 20μg/kg during the
pre-ovulatory period, with only two applications, was not able to increase the ovulation rate of
goats subjected to TAI.
09.043 - ACTIVATION OF NORADRENERGIC AND HYPOTHALAMIC NEURONS RELATED TO
INCREASED TAIL SKIN TEMPERATURE IN OVARIECTOMIZED RATS: INVOLVEMENT OF KISSPEPTIN
NEURONS
CRISTINA DOS SANTOS FONSECA; GLEISY KELLY NEVES GONÇALVES; RAPHAEL ESCORSIM SZAWKA;
ADELINA MARTHA REIS
Hot flushes are usually treated with estrogen replacement in postmenopausal women but the
neural pathways involved are poorly understood. We investigated the activation of brain areas
associated with the increase in tail skin temperature (TST) in an animal model of post menopause.
Rats were ovariectomized (OVX) and implanted with capsules containing 17ß-estradiol (E2)
(OVX+E2) or corn oil (OVX). TST was recorded for 2 h at ambient temperature at 3, 7 and 14 days
after surgery. On day 15th, rats were perfused and the brains, processed for
immunohistochemistry. On day 14 after ovariectomy, the TST was higher in OVX rats than in
OVX+E2 rats (P<0.05). In the brainstem, OVX rats displayed more Fos/tyrosine hydroxylase
doubled-labeled neurons in the A1, A2 and locus coeruleus compared with OVX+E2 rats (P<0.05).
OVX rats exhibited more Fos-ir neurons than OVX+E2 rats in the rostral preoptic area (POA),
anteroventral periventricular nucleus (AVPV), median preoptic nucleus (MnPO) and
paraventricular nucleus (PVN). Furthermore, OVX rats displayed a greater number of kisspeptin
neurons in the arcuate nucleus expressing Fos than OVX+E2 rats (P<0.05). There was a strong
correlation between the TST and Fos expression in the A1, A2, AVPV and POA. Thus, ovariectomy
gradually increased TST associated with neuronal activation in the brainstem and hypothalamus,
suggesting activation of ascending noradrenergic inputs. Kisspeptin neurons seem to be activated
in this pathway and therefore may play a role in E2 modulation of thermoregulatory vasomotion.
09.044 - EFFECT OF SUPPLEMENTATION WITH CONJUGATED LINOLEIC ACID ON BODY
COMPOSITION AND PLASMA PARAMETERS OF DIABETIC RATS
ANGELICA NOVI CAPELASSI; THIENE MONTANI LEAL; PAULA GIMENEZ MILANI; CECÍLIA EDNA MAREZE
DA COSTA
There is little information in the literature concerning the effects of supplementation with
conjugated linoleic acid (CLA) on body composition and plasma parameters of diabetic population.
In this study, experimental diabetes was induced in 60-day-old male Wistar rats by intravenous
injection of streptozotocin (45 mg/kg). Animals with fasting blood glucose levels greater than or
equal to 200mg/dl were selected. Then, four experimental groups were established: 1) NC (normal
control, n = 5); 2) NT (normal treated, n = 5); 3) DC (diabetic control, n = 19); 4) DT (diabetic
treated, n = 19). The NT and DT groups received CLA (0.04 mg / 100g pc, Ultimate Nutrition USA) by gavage for 25 days. In normal and diabetic animals, CLA supplementation reduced (p<0.05)
body fat from 20 to 30%, with a more marked reduction in the retroperitoneal adipose tissue. The
brown adipose tissue was not affected by CLA supplementation. The body weight gain was lower
(-24%) in normal animals that received CLA. Diabetic animals treated with CLA had reduced fasting
plasma glucose (261.8 ± 18.5 vs 215.3 ± 12,4mg / dl), triglycerides (61.8 ± 5.3 vs 48.7 ± 2.9 mg /
dl), VLDL (12.3 ± 1.1 vs. 9.7 ± 0.6 mg / dL) and AST levels (60.1 ± 2.5 vs. 53.2 ± 2.1 U / L).
Conclusion: supplementation with CLA improves glycemic and lipid control and reduces adiposity
in diabetic rats.
09.045 - P110γ/AKT SIGNALING MEDIATES THE ANTIPROTEOLYTIC EFFECTS OF β2-ADRENERGIC
AGONIST IN MOUSE SKELETAL MUSCLE
DAWIT ALBIEIRO PINHEIRO GONÇALVES1; LEANDRO HENRIQUE MANFREDI1; WILIAN ASSIS SILVEIRA1;
FLÁVIA APARECIDA GRAÇA1; DANILO LUSTRINO1; ENRICO BERTAGGIA2; MARCO SANDRI2; ISIS DO
CARMO KETTELHUT1; LUIZ CARLOS CARVALHO NAVEGANTES1
1.RIBEIRÃO PRETO MEDICAL SCHOOL-USP, RIBEIRÃO PRETO, SP, BRASIL; 2.VENETIAN INSTITUTE OF
MOLECULAR MEDICINE, PADUA, ITALIA.
This study was undertaken to identify: 1) whether autophagy-lysosomal (ALS) and ubiquitinproteasome (UPS) systems, two major proteolytic systems, are regulated by Formoterol (FOR), a
selective β2-adrenergic agonist (BA); and 2) the intracellular signaling pathways required for such
effects. For that, 2-day fasted mice received an injection of FOR (300 μg/kg; sc) or saline and
tibialis anterior (TA) muscles were harvested. Proteasome activity was evaluated in TA transfected
in vivowith the reporter Ub G76V-GFP. This reporter is targeted to proteasomal degradation, and
the GFP fluorescence is detected by microscopy in a high level when proteasome function is
inhibited by FOR at 2h. At 1h, FOR reduced (80%) LC3-II/LC3-I ratio, indicating the inhibition of
autophagosome formation. At 4h, FOR suppressed (~40%) Foxo activity in vivo, as analyzed by
reporter luciferase assay, and the mRNA levels of Foxo target genes related to UPS and ALS, as
measured by qPCR. At ½ h, FOR elevated the phosphorylation (p) levels of Akt (~10x), Foxo1 (2x)
and CREB (2x), a canonical target of adrenergic signaling. Notably, FOR-induced Akt activation
was blunted in p110γ KO mice. In addition, in vivo overexpression of dominant negative form of
Akt prevented FOR effects. Altogether, our data show that BA counteracts protein catabolism
induced by food deprivation by downregulating the Foxo-mediated atrophy gene program and the
activities of UPS and ALS, effects that require p110γ/Akt signaling.
09.046 - ESTRADIOL REVERSES HYPERTROPHIC EFFECT OF ET-1 IN CARDIOMYOCYTE:
INVOLVEMENT OF GPER1 PATHWAY
GLEISY KELLY NEVES GONÇALVES; FERNANDA SANTOS MENDES; ANA PAULA ALVES; EDUARDO
NOCCHI; SÉRGIO RICARDO ALUOTTO SCALZO JÚNIOR; SILVIA CAROLINA GUATIMOSIM FONSECA;
ADELINA MARTHA REIS
Estradiol (E2) plays an important role in the modulation of cardiac hypertrophic responses.
Previous data have shown that E2 prevents cardiomyocyte hypertrophy induced by endothelin-1
(ET-1) and Angiotensin II, acting through the increase of atrial and type-B natriuretic peptides
(ANP and BNP) levels. In recent years, a membrane-bound E2 receptor has emerged, G proteincoupled estrogen receptor 1 (GPER1), which mediates many of the effects of estrogens. In this
work, we investigated whether E2 protective signaling effect on ET1-induced hypertrophy is
mediated via GPER1. Cardiomyocytes were isolated from newborn female rats and cultured in
DMEM supplemented with 10% fetal bovine serum with antibiotic and antimycotic. Cells were
treated for 48h with ET-1 (100 nM) in the presence or not of E2 (10 nM). In some experiments
GPER1 antagonist (G15, 10 nM) was added to the medium. ET-1 induced an increase in cardiac
myocyte area compared with control group (1688.4 vs 737.1 µm2). Treatment with E2 inhibited
the hypertrophic effect of ET-1 (34.7%). Treatment with G15 reversed antihypertrophic effect of
E2. ET-1 upregulated ANP and BNP levels, effect abolished by E2. G15 prevented the decrease of
BNP but not ANP in cardiomyocytes treated with E2 in the presence of ET-1. Moreover, ET-1
induced the reduction of the contraction frequency and increase of contraction force in
cardiomyocytes that was reversed by E2. In conclusion, E2 plays a key role antagonizing the
hypertrophy and contractile profile induced by ET1 and part of this effect was mediated by GPER1.
09.047 - BODY COMPOSITION, SLEEP ARCHITECTURE AND METABOLIC PROFILE OF SEDENTARY
OBESE INDIVIDUALS WITH MILD AND SEVERE OBSTRUCTIVE SLEEP APNEA
CAMILA MARIA DE MELO; MARIANA PANTALEÃO DEL RE; MARCUS VINICIUS LUCIO DOS SANTOS;
HANNA KAREN MOREIRA ANTUNES; SERGIO TUFIK; MARCO TULIO DE MELLO
UNIVERSIDADE FEDERAL DE SÃO PAULO, SÃO PAULO, SP, BRASIL.
Introduction: The relationship between obstructive sleep apnea (OSA) and obesity is well known.
Few studies evaluated body composition of obese with OSA. Objective: Compare body
composition, sleep architecture and metabolic profile of obese individuals with mild and severe
OSA. Methods: 35 obese men, 40.5±7.7 years were submitted to a basal polysomnography, body
composition evaluation and blood collection. Results: 10 individuals were classified as mild OSA
(AHI >5<15 events/h) and 25 with severe OSA (AHI > 15events/h). No differences in
anthropometric, body composition and metabolic profile were found between groups (Body mass:
108.1±10.7 and 108.1±16.0 kg; BMI: 34.6±3.9 and 35.1±4.0 kg/m2, Body fat: 37.8±6.3 and
38.8±5.3%, Fat free mass: 62.2±6.3 and 60.9±5.5% in mild and severe OSA, respectively. Severe
OSA presented higher N1 (12.8±5.9% versus 24.6±17.3% in severe, p=0.004) and lower N3
(23.2±6.7% versus 13.4±10.17%, p=0.009) in relation to mild OSA. The anabolic hormone IGF-1 was
diminished in severe OSA (178.6±30.6 and 133.2±39.6ng/mL in mild and severe OSA, p=0.005). A
43
negative correlation was found between IAH and IGF-1 in all subjects (r=-0.554, p=0.002). In
severe OSA patients a negative correlation between IAH and % of fat free mass was found (r=0.428, p=0.033). Conclusion: There is no difference in body composition between mild and severe
OSA patients. Severe OSA is associated with higher N1 and lower N3 sleep stages and lower levels
of IGF-1. Higher IAH levels could be associated with lower fat free mass content.
09.048 - EXPOSURE TO BISPHENOL A DISRUPTS THE HYPOTHALAMUS-PITUITARY-THYROID AXIS
RODRIGO RODRIGUES DA CONCEICAO1; MARINA MALTA LETRO KIZYS1; KELEN CARNEIRO OLIVEIRA1;
JANAINA SENA DE SOUZA1; MARCO AURELIO ROMANO2; RENATA MARINO ROMANO2; GISELE
GIANNOCCO1; MAGNUS RÉGIOS DIAS DA SILVA1; MARIA IZABEL CHIAMOLERA1
1.UNIVERSIDADE FEDERAL DE SÃO PAULO, SAO PAULO, SP, BRASIL; 2.UNIVERSIDADE ESTADUAL DO
CENTRO-OESTE, GUARAPUAVA, SP, BRASIL.
Bisphenol A (BPA) is one of the major compounds in plastic materials and it has been associated
with endocrine disruption . Alterations in thyroid hormone axis have also been reported; in vitro
and in vivo experiments showed that BPA acts as an antagonist to thyroid hormone in thyroid
hormone receptors (TRs). The aim of the present study is to analyze BPA possible disruptive effects
on hypothalamus-pituitary - thyroid axis at the level of gene expression classically regulated by
thyroid hormone in vivo. In this study, prepubertal male Wistar rats were exposed to 5 and 25
mg/kg/day BPA from postnatal day 23 (PND23) to PND58 and sacrificed at PND102. Serum
concentrations of TSH, T3 and T4 were assessed. Total RNA was extracted from hypothalamus,
pituitary, liver and heart. Gene expression analysis was performed for the type 1, 2 and 3
deiodinase (Dio1, Dio2 and Dio3), and the thyroid hormone transporter Mct8. Animals exposed to
BPA presented a drift towards TSH elevation, paradoxically to an increase of total T3 and a
reduction of T4. In pituitary tissue, Mct8 gene expression was decreased in animals exposed to
BPA lower dose and Dio3 gene expression was repressed in both BPA doses. Dio2 gene expression
were elevated. In summary, BPA-exposed animal presented a similar hormonal pattern of humans
with MCT8 mutantions described in the literature. While, the gene expression profiles are similar
to what it is expected for hypothyroid animals. Notwithstanding, BPA could also play a role in the
complex regulatory mechanism of gene expression associated to thyroid hormone
09.049 - AVALIAÇÃO DOS PARÂMETROS REPRODUTIVOS DE RATAS SUBMETIDAS À DESNUTRIÇÃO
PROTEICA GESTACIONAL
ELISA BARROS DE ANDRADE; RAYSSA GABRYELLA NERY DE BARROS; ISABELA CATARINA LEÃO DA
COSTA; GABRIELA FREIRE DE ALMEIDA VITORINO; JOSÉ LUIZ DE BRITO ALVES; VIVIANE DE OLIVEIRA
NOGUEIRA; MONIQUE ASSIS DE VASCONCELOS BARROS; JOÃO HENRIQUE DA COSTA SILVA
UNIVERSIDADE FEDERAL DE PERNAMBUCO, VITÓRIA DE SANTO ANTÃO, PE, BRASIL.
Tem sido demonstrado que déficit nutricionais durante a gestação pode induzir alterações útero
placentário e afetar a capacidade reprodutiva da mãe. Assim, objetivamos investigar os efeitos
da desnutrição proteica gestacional sobre os parâmetros reprodutivos de ratas. A partir do
primeiro 1º de prenhez, ratas Wistar foram divididas em dois grupos: um que recebeu a dieta
normoproteica (NP: 17% de caseína, n=8) e o outro, uma hipoproteica (HP: 8% de caseína, n=14).
Durante a gestação (G), o ganho de peso e o consumo de ração foram averiguados. No 20º dia de
G, as ratas foram laparatomizadas para análise dos parâmetros reprodutivos. Houve aprovação
pelo comitê de ética da UFPE (2014-92). O grupo HP apresentou, durante a 1ª semana de G, maior
consumo de ração (NP: 111±14 vs. HP: 146± 9g; p=0,04) e durante a 2ª semana, menor consumo
de água (NP: 177±7 vs. HP: 151±7mL; p=0,02). O ganho de massa corpórea foi similar entre os
grupos. Com relação aos parâmetros reprodutivos, o nº de fetos vivos (NP:61 vs. HP:127), mortos
(NP: 0 vs. HP:15), relação feto/mãe (NP:8±5 vs. HP:8±5), massa dos fetos (NP: 2,3±0,2 vs.
HP:2,6±0,2g), peso das placentas (NP: 37±4vs.HP:38±3mg), dos ovários (NP:37,9±3,65 vs. HP:
37,16±4,5 mg), nº de implantações (NP:92vs.HP:164), reabsorções (NP:3vs.HP:23), implantações
viáveis (NP:89vs.HP:141) corpos lúteos (NP:13,9±0,8vs.HP:12,6±1,1) foi semelhante entre os
grupos. Assim, podemos concluir que a desnutrição proteica parece não alterar os parâmetros
reprodutivos em ratas.
09.050 - PREDICTING LITTER SIZE USING WEIGHT GAIN OF PREGNANT RATS: EFFECTS OF
MATERNAL THYROID STATUS AND DIET
DEMETRIUS SILVA DE SANTANA; VANESSA CIBELLE BARBOZA DE CARVALHO; DAMYRES MENEZES
SANTOS DE JESUS; LOIC HERNANDEZ DO AMARAL ARAGÃO; MAURÍCIO SILVA SANTOS; PATRÍCIA
RABELO DOS SANTOS; EDÊNIA DA CUNHA MENEZES; IURA GONZALEZ NOGUEIRA-ALVES; DANIEL
BADAUÊ-PASSOS, JR.
UNIVERSIDADE FEDERAL DE SERGIPE, SÃO CRISTÓVÃO, SE, BRASIL.
We aimed to estimate litter size given body weight variations throughout rat’s pregnancy and also
to analyze the effects of maternal thyroid hormones and diet in such prediction. We used data
obtained from previous work in which pregnant rats were subjected to two factors: i) treatment
with methimazole 0.02% in drinking water from gestational day (GD) 9 to birth or vehicle (tap
water); and ii) westernized or control diet from GD 3 to birth. Maternal body weight was daily
recorded from 4th to last day of pregnancy in 39 rats. Litter size was measured at birth. Data was
analyzed with algorithm programmed in R language. Correlogram between weight gain during all
possible intervals and litter size has revealed that best correlation was seen at the interval from
GDs 14 and 19 (Pearson’s R = 0,892). Linear regression was performed using weight gain for
predicting litter size. Angular coefficient was 0.279 ± 0.024 and linear coefficient was -0.447 ±
0.927. Residuals were negatively skewed in small litters, and positively in big litters. Residuals’
skewness was virtually absent in intermediate-sized litters. Both factors did not affect the results
(p > 0.05 in univariate linear model, for main effects and interaction). We conclude that it is
possible to reasonably estimate litter size given weight gain of pregnant rats. It may be especially
useful for studies of fetal programming.
09.051 - EFFECT OF CHRONIC STRESS IN METABOLIC PARAMETERS OF RATS FED WITH COMFORT
FOOD
SARA PEREIRA DA SILVA; DANIELA ORTOLANI; REGINA CELIA SPADARI; MARCIA CARVALHO GARCIA
UNIFEP, SANTOS, SP, BRASIL.
Objective:To investigate the effects of chronic unpredictable mild stress (CUMS) on metabolic
parameters in rats with access to comfort food (CF). Method and Results: Adult, male Wistar rats
were distributed in 4 groups: control (C) and CUMS, both fed with commercial chow (CC), control
and CUMS with access to CC and (CF). Food intake, corticosterone, glucose, insulin, leptin, TG,
LDL and the percentage of body fat were determined. Groups were compared by ANOVA plus
Bonferroni test; differences were significant when p<0.05). Rats in all groups preferred CF.CUMS
reduced the intake of CC (24.6±0.2; 19.9±0.7g) and CF (27.1±0.6; 23±0.8g). Corticosterone
increase induced by CUMS (226.8±24.4 vs 580.5±98.8) were attenuated by CF (210.0±62.5 vs
367.3±21.7mg/dl). Glicemia (111.0±3.5 vs 116.5±2.8), insulinemia (1.2±0.1; 1.3±0.1), TG
(150.7±15.0; 133.4±6.1md/dl), and LDL(111.4±4.6; 133.5±5) were not altered by CUMS. Glicemia
(124.8±2.2; 120.0±6.9), TG (187.8±11; 158.7±14.1), LDL (134,4±5,4; 130,3±9,5) were higher,
whereas insulinemia (1.3±0.1; 0.9±0.2) was reduced in rats fed CF. CUMS reduced subcutaneous
(7.7±1.0; 6.3±0.9%) and visceral (8.7±1.5; 7.6±0.9%) adipose tissue in rats fed with CC and fed
with CF (13.0±1.1; 9.0±1.1 and 15.9±1.0; 12.7±1.1%, respectively). Leptin were not altered by
CUMS (4.1±0.5; 7.4±1.2ng/dl) but were increased by CF (23.3±2.6; 17.7±1.9). Conclusion: Lipolytic
effects of CUMS. The CF diet installed the lipid and glucose metabolism disorders, as well the
hyperleptinemia and lipogenic conditions.
09.052 - LOW MATERNAL THYROID HORMONES DURING GESTATION DISRUPT THYROID STATUS
IN OFFSPRING RATS
VANESSA CIBELLE BARBOZA DE CARVALHO1; PATRÍCIA RABELO DOS SANTOS1; HELAINE CRISTINA
BATISTA DOS SANTOS1; DANIELLE PEREIRA GAUJAC1; IURA GONZALEZ NOGUEIRA-ALVES1; NAYANA
COUTINHO RODRIGUES2; MICHELE PORTO MARASSI2; DANIEL BADAUÊ-PASSOS, JR.1
1.UNIVERSIDADE FEDERAL DE SERGIPE, SÃO CRISTÕVÃO, SE, BRASIL; 2.UNIVERSIDADE FEDERAL
RURAL DO RIO DE JANEIRO, SEROPÉDICA, RJ, BRASIL.
Maternal thyroid hormones are essential for fetal development. We investigated the impact of
experimental gestational hypothyroidism (EGH) on plasma level of total tri-iodotironin (TT3) and
total thyroxine (TT4) in offspring. EGH was induced by the administration of 0.02% methimazole
(MMI) in drinking water from the 9th day of gestation until birth. Blood samples were collected in
pregnant mothers on gestational days (GD) 0 and 21; and in offspring on 60 and 120 postnatal day
(PND). The statistical analysis was performed using two-way ANOVA. MMI reduced TT3 (7.4 ± 0.2
ng/mL vs.5.9 ± 0.6 ng/mL, p<0.05, at GD 21) and TT4 serum levels in pregnant rats (12.2 ±1.5
mg/dL vs. 4.6 ± 0.9 mg/dL, p<0.001, at GD 21) [For TT3: main effect for interaction, F(1,4.89) =
2.99; p<0.05; and for TT4: main effect for interaction, F(1,21.41) = 78.34; p<0.001]. Males and
females offspring from MMI treated dams (OMTD) showed no differences in TT4 when compared
to offspring from water treated dams (OWTD). However, TT3 was higher in males OMTD on PND
60 (32.4 ± 2.2 ng/dL vs 42.7 ± 4.5 ng/dL) and lower on PND 120 (56.2 ± 3.3 ng/dL vs 44.9 ± 1.7
ng/dL) when compared to OWTD [For TT3: main effect for interaction, F(1,33)=13.52; p<0.0001].
No changes were seen in TT3 and TT4 serum levels of females OMTD. In conclusion we
demonstrated that 0.02% MMI administration during gestation induces hypothyroidism in pregnant
rats and disrupts TT3 profile in male offspring, but not in female.
09.053 - EFECTS OF CHRONIC CORTISOL TREATMENT IN WHITE ADIPOSE TISSUE
PATRICIA PEREIRA NUNES; SANDREA ANDREOTTI; ROGERIO ANTONIO LAURATO SERTIÉ; FABIO BESSA
LIMA
INSTITUTO DE CIÊNCIAS BIOMÉDICAS - ICB-USP, JUNDIAI, SP, BRASIL.
Chronic glucocorticoid (GC) treatment promotes increase of central adipose tissue depots. We
created an experimental condition of chronic cortisol exposure to investigate its effect on white
adipose tissue and glucose metabolism. Methods: Male Wistar rats (n=19) were divided into control
(CON) and cortisol-treated (CORT) groups. CORT received hydrocortisone hemisuccinate (0,6
mg/kg/day) during 6 weeks, while CON received saline. Food and water intake and body mass gain
were mesured weekly. After 5 weeks an oral glucose tolerance test (oGTT) was applied; when
completed 6 weeks they were killed. Retroperitoneal (RP), mesenteric (ME), omental (OM) and
inguinal subcutaneous (SC) fat pads were extracted, weighed and processed for protein analysis.
Results: There was no significant difference between groups for body mass gain, food and water
intake, and RP and ME visceral fat pads mass. However, SC and OM fat pad mass of CORT increased
significantly (2,60±0,16g; 0,19±0,02g respectively; n=8) when compared to CON (2,05±0,09g;
0,11±0,01g respectively, n=11). The oGTT test did not differ between groups. Conclusion: Despite
the increase of SC and OM fat pad mass, cortisol treatment did not alter other parameters
commonly observed in long-term treatment with GC: body mass, food intake and glucose
tolerance, possibly because even the exposure to cortisol was chronic, the dose may not be high
enough to break the circadian rhythm of the hypothalamic-pituitary-adrenal axis, which we are
currently evaluating.
09.054 - INCREASE IN VSC-PRODUCING BACTERIA AND RELATIONSHIP WITH STRESS, IN HEALTHY
VOLUNTEERS
PATRICIA OLIVEIRA DE LIMA; BRUNO DIAS NANI; MICHELLE FRANZ-MONTAN; CAROLINE MORINI
CALIL; ANTONIO BENTO ALVES DE MOARES; KARINA COGO- MÜLLER; FERNANDA KLEIN MARCONDES
FACULDADE DE ODONTOLOGIA DE PIRACICABA - UNICAMP, PIRACICABA, SP, BRASIL.
Around 90% of all bad breath odors originate in the mouth from bacterial amino acid metabolism,
producing volatile sulfur compounds (VSC). However, besides oral diseases, stress and the female
menstrual cycle have also been associated with its origin. The aim of this study was to quantify
the mainly VSC –producing bacteria, in saliva samples of women students enrolled at Piracicaba
Dental School, University of Campinas, Brazil. Two groups were included in this study: 19 stressed
and 33 non-stressed students. Real-time PCR technique was performed to quantify the amounts
of 4 VSC –producing bacterial species: Fusobacterium nucleatum (Fn), Solobacterium moorei (Sm),
Porphyromonas gingivalis (Pg), Tannerella forsythia (Tf), besides Universal bacteria (U). Data were
compared by Mann-Whitney test (p<0.05). Women under stress showed higher amounts (log
median - interquartile range) of Fn (4.3 - 0.3 vs 3.9 - 0.5, p = 0.0003) and U (6.8 - 0.6 vs 6.5 - 0.3,
p = 0.01) compared to non-stressed volunteers. There were no significant differences in the
quantities of species Sm (4.6 - 1.0 vs 4.7 – 0.9, p> 0.05), Pg (1.4 - 1.0 vs 1.3 - 1.2; p> 0.05) and Tf
(1.6 - 1.5 vs 1.7 - 2.0; p> 0.05) between the groups. Results show that stress induced changes in
the total oral microbiota and suggest that Fn bacterial species may be involved in the production
of VSC in healthy women under stress. Further studies are needed to confirm this hypothesis.
09.055 - NEONATAL THYMECTOMY RESULTS IN CHANGES ON THE BONE MINERAL DENSITY AND
SKELETAL GROWTH OF RATS
WAGNER GARCEZ MELLO1; NOÉLLE EGÍDIA WATANABE KIILL2; GISELI MITSUY KAYAHARA1; OLÍVIA
BORGHI NASCIMENTO1; NARAYANA GUIMARÃES MARQUI 1; RITA CÁSSIA MENEGATI DORNELLES2;
MARCELO MACEDO CRIVELINI2; JOSÉ ANTUNES-RODRIGUES3; JOÃO CESAR BEDRAN DE CASTRO2
1.FOA-UNESP / UNITOLEDO, ARACATUBA, SP, BRASIL; 2.FOA-UNESP, ARACATUBA, SP, BRASIL;
3.FMRP-USP, RIBEIRÃO PRETO, SP, BRASIL.
Several studies confirm the involvement of the thymus in the developmental programming of the
neuroimmunoendocrine functions of the hypothalamus, however, the thymus’s ability to
influence, directly or indirectly, skeletal development and bone quality are still unclear. Thus,
this study investigated if there are alterations in the skeletal sexual dimorphism and bone quality
during adulthood after neonatal thymectomy. To this, Wistar rats, newborns, were divided per
sex into control and neonatally thymectomized groups (n = 08 per group). Experimental pups were
cryoanesthetized, thymectomized by 3 days after birth, and were observed for 120 postnatal days.
Right femurs were collect and the length and thickness of the femurs were performed with aid of
a caliper; after measuring, the areal bone mineral density (aBMD) was determinate by dual-energy
X-ray absorptiometry (DXA). Data were analyzed using Two-Way ANOVA and Bonferroni post-test
and expressed as the means±SE. The results show that only in male rats, neonatal thymectomy
during the critical period of life, induce a long-lasting modification in skeletal development, since
thymectomized group has a higher femoral length and total bone area than control group,
however, areal bone mineral density was reduce because the bone mineral content acquisition
was impaired in neonatally thymectomized male. These results provide new insight into the
dynamic complexity the skeletal sexual dimorphism.
09.056 - PERSISTENT ENDOCRINE CHANGES IN SEPSIS SURVIVING ANIMALS
NILTON NASCIMENTO SANTOS-JÚNIOR; LUIS HENRIQUE ANGENENDT DA COSTA; CARLOS HENRIQUE
ROCHA CATALÃO; RODRIGO PEREIRA DE ALMEIDA RODRIGUES; JOÃO PAULO MESQUITA LUIZ;
ALEXANDRE KANASHIRO; MARIA JOSÉ ALVES ROCHA
USP, RIBEIRAO PRETO, SP, BRASIL.
During sepsis, cytokines, nitric oxide, and other compounds are liberated, which may contribute
to the cognitive alterations observed during and after the disease. These substances may also be
responsible for the hormonal secretion alterations that occur during sepsis. Oxytocin and
corticosterone secretion are increased in the early phase of sepsis but in late phase are impaired.
However, it is not known yet if hormonal impairment persist after surviving sepsis. We investigated
hormonal secretion (oxytocin and corticosterone) in sepsis surviving animals. The experimental
protocol was approved by the Institutional Ethical Committee for use of animals
Male Wistar rats (250-300g) were submitted to sepsis by cecal ligation and puncture (CLP), or left
as control (naive). After 5 days these animals received a 2ml i.v. injection of lipopolysaccharide
(LPS, E. coli (055: B5, Sigma, 5 mg/kg) or vehicle (isotonic saline). Following 2 hours, the animals
were decapitated (n=5-8/group) and the blood was collected for plasma hormonal analysis by
ELISA. As expected following two hours of LPS injection there was an increase in plasma oxytocin
(p<0,001) and corticosterone levels (p<0,05) compared to vehicle in both groups (survival and
naive). However, the increase in oxytocin secretion was lower (p<0,05) in the surviving animals
(32,6 ±5) compared to naive group (56,7 ± 6,6).
We conclude that sepsis surviving animals has a persistent Impairment in the oxytocin
secretion caused by endotoxemic stimulus.
09.057 - DIETARY SULFUR AMINO ACID RESTRICTION REQUIRES DICER FUNCTION TO CONFER
ITS BENEFICIAL EFFECTS
BEATRIZ ALVES GUERRA1; BRUNA BRASIL BRANDAO1; SILAS PINTO DA SILVA1; CARMEN E. PERRONE2;
THIAGO MARTINS BATISTA3; EVERARDO MAGALHÃES CARNEIRO3; MARCELO A. MORI1
1.UNIFESP/EPM, SAO PAULO, SP, BRASIL; 2.ORENTREICH FOUNDATION FOR THE ADVANCEMENT OF
SCIENCE, NEW YORK, ESTADOS UNIDOS; 3.UNICAMP, CAMPINAS, SP, BRASIL.
44
Dietary restriction (DR) confers beneficial health effects and prolongs lifespan. It has been
reported that DR prevents the decline in adipose tissue expression of microRNAs and their
processing enzymes, particularly Dicer, that occurs with aging. To test whether specific nutrient
restriction was responsible for Dicer upregulation upon DR in adipose tissue, we performed a
nutrient screen in mouse preadipocytes and identified that methionine or cysteine restriction was
sufficient to increase Dicer mRNA expression in these cells. Consistently, mice subjected to DR
supplemented with methionine, cysteine or casein had adipose tissue Dicer expression levels
similar to ad lib fed mice. The effect of DR on adipose tissue expression of Dicer was also partially
blocked by treatment of mice with propargylglycine, an inhibitor of cystathionine γ-lyase,
suggesting that activation of the transsulfuration pathway is involved in the regulation of Dicer
expression. Consistently, treatment of mouse C3H10T1/2 differentiated adipocytes with the
hydrogen sulfide donor NaHS was sufficient to increase Dicer expression. Methionine restriction
(MR) in rodents resulted in weight loss and increased expression of Dicer in adipose tissue. Most
importantly, MR increased UCP1 expression in the subcutaneous adipose tissue of mice and this
was abrogated by fat-specific Dicer knockout. Finally, both DR and a MR-mimetic protocol
extended lifespan in C. elegans in a Dicer dependent manner. Here we propose a new mechanism
through which DR leads to metabolic adaptation and confers beneficial health effects.
09.058 - RESVERATROL REDUCES PROTEOLYSIS IN MUSCLE EDL INDEPENDENTLY OF AKT/FOXO1
IVANILDO INÁCIO DA SILVA JÚNIOR1; FLÁVIA PEIXOTO ALBUQUERQUE1; AMANDA MOREIRA DE
ALBUQUERQUE1; NEUZA MARIA ZANON2; SILVIA DE PAULA GOMES2; DAWIT ALBIEIRO PINHEIRO
GONÇALVES2; ISIS DO CARMO KETTELHUT2; LUIZ CARLOS CARVALHO NAVEGANTES2; DAYANE
APARECIDA GOMES1; EDUARDO CARVALHO LIRA1
1.UNIVERSIDADE FEDERAL DE PERNAMBUCO, RECIFE, PE, BRASIL; 2.FACULDADE DE MEDICINA DE
RIBEIRÃO PRETO, RIBEIRÃO PRETO, SP, BRASIL.
Objective: Evaluate the Akt/FoxO1 pathway in antiproteolytics effect of Resveratrol in skeletal
muscle from denervated rats. Methods: Bilateral motor denervation (DEN) was induced by sciatic
nerve section in male Wistar rats (±80g). At three days after surgery, the proteolysis was estimated
in extensor digitorus longus (EDL) incubated in the presence, and absence, of Resveratrol (100µM).
The release of tyrosine to the incubation medium was used as a marker of protein metabolism as
well as Western blot and real time PCR were used to measured protein and gene expression,
respectively. This study was approved by CEUA UFPE (23076.01234/2012-79). Results: The motor
denervation induced an increase in overall proteolysis (~60%) in the EDL, which was accompanied
by hyperactivity of ubiquitin-proteasome system (UbP, ~3X) and calcium dependent (~41%)
systems. Resveratrol reduced overall proteolysis in DEN (~28%) and sham (~39%) muscle,
throughout UbP (36%) and calcium dependent (~28%) proteolysis reduction in both groups. E3ligases overexpression was not detected at third day after denervation, however resveratrol
reduced Atrogin1 expression in sham and DEN rats, as well as MuRF1 in sham rats. Paradoxically,
this phytoalexin reduced Akt and FoxO1 phosphorylation, on the other hand resveratrol increase
the phosphorylation of AMPK and CREB. Conclusion: These data indicated that Resveratrol
attenuated skeletal muscle proteolysis through of inhibition of UbP and calcium activity and these
effects were independently of Akt/FoxO1 pathway and possibly mediated by the AMPK and CREB.
THEME 10 - PHYSIOLOGY EDUCATION
10.014 - EFFECT OF SALINITY ON HEART RATE OF POMACEA LINEATA NEONATES AS A
NONINVASIVE METHOD FOR ANIMAL PHYSIOLOGY STUDY
ANDRÉ LUCAS CORRÊA DE ANDRADE; STEPHANNIE CAROLINE BARROS LUCAS DA SILVA; PRISCILA
RAFAELA LEÃO SOARES; MARÍLIA CORDEIRO GALVÃO DA SILVA; THAMIRIS PINHEIRO SANTOS;
PABYTON GONCALVES CADENA
The use of animals is common for teaching, its use is to study and demonstrate about physiological,
anatomical and behavioral processes. However, methods that use gastropods to measure heart
rate in laboratory exercises could be difficult to arrange, time-consuming and involve invasive
procedures. In this way, the aim of this study was to use Pomacea lineata neonates as an animal
model of noninvasive practical classes in animal physiology. The experiments were carried out
with one animal per group of students. The neonates were acclimated in a liter of water with
different concentrations of NaCl (0, 0.75 and 1.5 w/v). During the observation period, the students
measured the heart rate of the animal three times at intervals of one-minute using
stereomicroscopes and under bright light to see the heart through the shell. This technique
replaces the procedure with adult animals, where is needed to break the shell to see the heart.
As results, the heart rate in the control group (72.8 ± 11.54) was higher than 0.75 (53.5 ± 9.2) and
1.5 w/v (44.2 ± 7.2) treatments. It was observed significant differences by Tukey test (p < 0.05)
between control group and experimental groups. There was no significant differences between
experimental groups (0.75 and 1.5 w/v). From these results, this laboratory exercise was efficient
to show the new noninvasive method of effect of salinity in P. lineata. Indeed, this exercise
showed how changes of osmotic concentrations promote variation in the heart rate.
10.015 - VIRTUAL FORUMS OF PHYSIOLOGY CURIOSITIES AS A SUPPORT TOOL IN HUMAN
PHYSIOLOGY TEACHING
RITHIELE GONÇALVES; BEN-HUR SOUTO DAS NEVES; MARCUS VINÍCIUS SOARES DE LARA; CAROLINE
DALLA COLLETTA ALTERMANN; PAMELA BILLIG MELLO CARPES
The use of technology tools in the teaching has been increase a lot. This study aimed to evaluate
the use of physiology curiosities forums in the Moodle virtual environment. The forums, about
curiosities regarding the contents of Human Physiology, were available along first curricular
semester of 2014. Among the topics included was: "Erythropoietin as doping in sport," "The spleen
and exercise", "Heart pacemaker"," Electrocardiogram", "Sports in the high altitude", and others.
To evaluate the impact of these forums in the physiology teaching was applied a questionnaire to
students. We observed a good student’s participation in all different forums. 91.5% of students
considered that the matters discussed in forums were directly related to the contents studied in
classes. 75.5% said that the topics contributed greatly to their understanding about how the
physiology is present in everyday life. 75.5% said that the themes increased their interest in
physiology. Also, 75% of students considered that the issues discussed in the forums contributed
to the increase of their learning. The average grade gave by students to this propose was 8 ± 1.96
(considering a scale from 0 to 10). The results indicate that the proposal reached its objectives,
providing an interesting teaching tool to promote the approximation of theoretical knowledge to
everyday life phenomenon.
10.016 - JOGO DIDÁTICO “BODY, A CONQUISTA DO CORPO HUMANO”, UMA FERRAMENTA
EDUCACIONAL CONSTRUÍDA POR MEIO DA INTEGRAÇÃO ENTRE UNIVERSIDADE E EDUCAÇÃO
BÁSICA
CAROLINA OLIVEIRA DE CERQUEIRA LIMA; TARCISO MAIA SANTOS; ÉRICA MARIA GRANJEIRO
STATE UNIVERSITY OF FEIRA DE SANTANA, FEIRA DE SANTANA, BA, BRASIL.
Estudos relatam a importância de haver a real inserção da Fisiologia no contexto escolar, visto
que isso ainda é pobremente trabalhado, especialmente na educação básica (Silveira da Rosa e
cols., Rev. Ciênc. Ext., 2013). Neste contexto, o Programa de Iniciação à Docência (PIBID) Biologia
da Universidade Estadual de Feira de Santana (UEFS) tem buscado estreitar o diálogo entre
Universidade e Educação Básica por meio da construção conjunta de diversos materiais didáticos
na área de Fisiologia. Assim, a adaptação dos conteúdos de Fisiologia Humana em um jogo
pedagógico, destacando aspectos imprescindíveis da Anatomia, Embriologia e Citologia Humana,
foi o objetivo principal da elaboração do jogo “Body, A Conquista do Corpo Humano”. A
metodologia é composta por um tabuleiro com a representação esquemática do corpo humano e
por perguntas formuladas sobre dez sistemas, incluindo sistema nervoso, endócrino, respiratório,
digestório, urinário, circulatório, reprodutor feminino e masculino. As perguntas são organizadas
no contexto do ensino facilitador da aprendizagem significativa. Esta metodologia pode ser
aplicada em sala de aula em grupos de no mínimo três e de no máximo seis jogadores, na ausência
ou presença de um moderador (professor). O jogo foi construído por meio da integração de
professores e alunos do Curso de Licenciatura em Ciências Biológicas da UEFS (PIBID) e por
professores do Colégio Estadual José Ferreira Pinto.
45
10.017 - MERGING SCIENCE AND ARTS TO TEACH CARDIOVASCULAR PHYSIOLOGY
CAMILO DE LELLIS SANTOS.; GABRIELA MONTEFERRANTE; VICTÓRIA OLIVEIRA; PATRÍCIA CASTRO;
MAYSA CRUZ
UNIFESP, DIADEMA, SP, BRASIL.
This study focused on developing an engaging, motivating and instructional integrated didactic
strategy based on different types of arts to teach cardiovascular physiology. In order to innovate
cardiovascular physiology teaching through pedagogic activities three types of arts (dance, music
and plastic arts) were chosen to cover major topics such heart rate, electrical cardiac property
and cardiac cycle. Thus students performed the movements of “heart dance” which represent
atrial and ventricular contractions following rhythms of classical, pop and samba songs during two
minutes each. After dancing, students used the application for smartphones - “Instant Heart Rate”
– to detect heart rate and significant differences were observed among rhythms, denoting normo,
bradi and tachycardia. To integrate electrical property and music, students were asked to identify
a correlation between the lyrics of “Eu sei” song, composed by Marisa Monte, and a cartoon of
the “Wizard of the OZ” that presents Tin Woodman expressing happiness due to its recently
acquired clock-heart. Finally, after making an origami heart that beats similar to cardiac
contraction, students were encouraged to write on it information about cardiovascular physiology
to be used as a cheat sheet during exams. All activities were appreciated by students, which selfevaluated as more engaged and motivated to study cardiovascular physiology.
10.018 - HEY, HO, LET'S GO! PUNK PEDAGOGY IN PHYSIOLOGY CLASSES FOR UNDERGRADUATE
BIOLOGY STUDENTS AT UFPB
PAULO FERNANDO GUEDES PEREIRA MONTENEGRO; WASHINGTON LUIZ DA SILVA VIEIRA; KLÉBER
DA SILVA VIEIRA
UFPB, JOÃO PESSOA, PB, BRASIL.
The “do-it yourself” punk ideology relates to autonomy and self-reliance. It may be used as a
pedagogical tool to stimulate sudents to be autonomous and independent by creating their own
learning environments, opportunities and professional path. During physiology classes for biology
undergraduate students at UFPB, we have an introdutory class to present the course plan, when
students are encouraged to discuss and propose changes to curriculum, schedule and evaluation
methods. We also have a class to discuss the steps in proposing a study project in physiology.
Then, different tools and techniques are shown and we discuss their use and feasibility. After
these two classes students are presented with case studies in physiology and are encouraged to
discuss and question their aims, methods and results. It i s also shown that creativity and handson ability is an essential feature of a physiologist, since it is usually necessary to create or adapt
equipment and experimental protocols. At the end of the discussions we show a video featuring
The Ramones playing “Blitzkrieg Bop” to illustrate the punk ideology. So far, students have
reported being stimulated by these teaching strategies to became more actively involved in
classes. The final messages for the students are: 1) punk pedagogy is education transformation
and innovation; 2) you should rely on inner strength and creativity to overcome adversity; 3) you
should develop autonomy to decide your professional path.
10.019 - O MANEJO DO ESTRESSE EM PROFESSORES DE ESCOLAS PÚBLICAS ESTADUAIS
ANA CAROLINA RIOS SILVINO1; MARIA CAROLINA MACHADO SILVA2; ANNA CAROLINA SANTOS PRADO
CAVANELLAS2; CLAUDIA DE MELO BENITES2; ISADORA BORGES MORATO DE ANDRADE2; MARINA
JUNQUEIRA NOLASCO2; REGIANE GRACIELE LOPES2; HERIKA I DE MESQUITA SAD3; MARIANA GONTIJO
RAMOS3; ANDRES MARLO RAIMUNDO DE PAIVA3
1.UNIVERSIDADE FUMEC, CONTAGEM, MG, BRASIL; 2.1PROFESSORES DA UNIVERSIDADE FUMEC,
BELO HORIZONTE, MG, BRASIL; 3.2ALUNOS DE INICIAÇÃO CIENTÍFICA DA UNIVERSIDADE FUMEC,
BELO HORIZONTE, MG, BRASIL.
O estresse constitui-se em um processo complexo de resposta do indivíduo a diferentes estímulos.
Um nível de estresse é necessário e saudável para o organismo, porém, é para a sobrecarga de
estresse que se deve chamar a atenção, quando este pode vir a tornar-se prejudicial. Algumas
profissões parecem ser mais vulneráveis ao estresse, a docência é uma delas. Com o intuito de
analisar os níveis de estresse e seus efeitos em professores da rede estadual de ensino em Belo
Horizonte foi desenvolvido um programa de manejo do estresse utilizando um procedimento de
intervenção (psicoeducação e técnicas de relaxamento). Os professores passaram por uma
avaliação inicial quantitativa dos níveis de estresse, medido pelo Inventário de Sintomas de Stress
de Lipp (ISSL), coleta de amostra salivar, frequência cardíaca e pressão arterial. Os participantes
foram instruídos a realizarem quatro sessões, uma vez por semana, de respiração profunda (15
minutos de relaxamento e 30 minutos de psicoeducação). Ao final de um mês, foram comparados
os níveis de estresse pré e pós intervenção. Observou-se uma redução significativa dos níveis de
estresse entre os participantes por meio do ISSL. Alterações na frequência cardíaca e pressão
arterial não foram significantes, porém análises preliminares do cortisol salivar mostraram uma
tendência a diminuição. Serão feitas, posteriormente, novas análises dos níveis de cortisol de
modo a elucidar a efetividade das técnicas aplicadas. Em suma, o estudo poderá contribuir para
a otimização e delineamento de medidas eficazes para controle de estresse.

poster sessions | sunday | august 2

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