life brochure soft copy

AFM for biological applications
LIFE setup and measuring head lifting mechanism
Development of AFM for biological applications is always a tricky problem because it requires
AFM integration with Inverted Optical Microscope.
LIFE is a fully automated, IOM-compatible system that saves all functionality of both AFM and IOM
AFM and IOM integration
Integration of two powerful research instruments is usually a point of compromise. IOM functionality is
often obstructed by AFM integration in terms of either optical methods available or limited condenser
aperture.
The LIFE is not the case. The whole variety of powerful IOM methods (dark field, phase contrast,
fluorescence imaging, etc.) is still available with LIFE. It is compatible with 0.55 NA IOM condensers and
ready for integration with Olympus IX71/IX81, Zeiss Axio Observer, Nikin TI. Integration with other IOMs
is available up on request.
DirectDrive cantilever excitation system
Amplitude modulation AFM modes are of great importance for investigation of soft biological specimens.
Because of low Q-factor of the cantilever and complex mechanical design of cantilever/sample holders, a
number of spurious resonances of the cantilever occur making it difficult to operate in AM-AFM modes in
liquid.
The LIFE uses specially developed DirectDrive cantilever excitation system that allows to avoid these
problems and to get clear cantilever resonance curve. Corresponding cantilever holder is completely
disassemble and washable, that allows to hold high level of cleanness during experiments.
Mica atomic lattice resolution,
LF-AFM. Scan size
Closed perfusion liquid cell.
Temperature range 5 .. 60 0C
LIFE replacable sample flexure
scanning stage
Fluid cell
The LIFE perfusion fluid cell provides sample temperature control in
the range from 10 to 60 0C. It operates with standard glass bottom
Petri dishes or regular microscopy cover glasses. Careful selection of
materials cell made from allows keeping high pureness of the
experiment. All fluid cells parts are fully washable.
6 scanning axis
PX Pro controller allows closed loop operation with up to 6 scanning
axis. Basic XYZ scanning is done by probe and provided by
decoupled flexure stage. In addition, either 100 100 µm XY stage or
100 µm Z stage can be installed into the base of the unit. One of
scanning axis is kept for IOM objective scanner.
DNA on mica surface, AM-AFM.
Scan size 650 650 nm
Automation and precision
The LIFE is a fully automated AFM. Automation of laser-cantileverphotodiode optical chain alignment is very convenient for operation in
liquid, where such alignment is quite tricky usually. Automated XY
sample positioning table provides fast, precise and easy sample
positioning. In addition, XY sample table can be equipped with optical
.
encoders that allows precise scanning of up to ~ mm field with AFM
Specially designed LIFE measuring head lifting mechanism makes
sample/cantilever exchange much easier compare to other
commercial biological AFMs.
Human blood cells, AM-AFM in
liquid. Scan size 16 16 µm
Panoramic optical view, MultiScan and
ScanStitch
Nova PX software provides many features based on high automation
level of the LIFE. Panoramic optical view allows collection of high
resolution, large scale images of the sample and then operation with
these data is the same as one operates with interactive maps.
Multiscan and ScanStitch provide easy acquisition of scans with ~
mm scan size and overlay AFM scans with the optical image
E. Coli cluster: AFM topography (left, scan size 15 15 µm) and its
superimposition on top of 50 50 µm fluorescense image (right)
Osteoblast cells in buffer solution.
Scan size 100 100 µm
Live tunica mucisa oris in buffer
solution, RSI adhesion map.
20 20 µm
Specification
C.0,2
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General
Modes
IOM
compatibility
9#/,'2#.'24
;-/1$,)*,#3+%4
Zeiss Axio Observer: Axio Observer
<,-%#'34
Olympus: IX product line
in air and liquid:
M/1$#%"0,)/.0"$-%#.')MHC)
Contact AFM
Nikon: TE 200, TI
(*%&&+&,!-.-/01
;=-''#'3)%:1,4
5-'3,4
AFM
measuring head
CL scan range: 100 100 20 µm
Linearity: 0.03%
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OBD sensor alignment: automated
>.#2,)A)B5C;()DE&DEEE)<F)7-'0*#0%+G4
OBD sensor laser wavelength: 850 nm
Motorized
C.6#'34 stage
Sample positioning range: 20 20 mm
5-'3,()?@4
Measuring head positioning range: 3 3 mm
C#')2%,14
Min step: 0.3 µm
Optical encoders: available up on request
5,2.$"%#.'4 !2!31
H.="2#'34
Replaceable sample scanning stages
A../4
XY scanning range: 100 100 µm
Z scanning range: 100 µm
<-I0',224
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Fluid cell
Perfusion and gas flow: available
;#F,4 Temperature Range: 10 .. 60 0C
K,#3+%4
INTEGRATED SOLUTIONS FO NANOTECHNOLOGY
Topography
Force
Feedback
MHC)21,=%I.2=.1:)
Lateral Force (LFM)
Force Modulation (FMM)
Amplitude modulation AFM
5-2%,I);1I#'3)N/-3#'3
)
Topography
;1I,-0#'3)5,2#2%-'=,)N/-3#'3
Phase
C-3',%#=)H.I=,)C#=I.2=.1:)
Feedback
AFM spectroscopy
Force-distance
J$,=%I.2%-%#=)H.I=,)C#=I.2=.1:
in air:
AFM '67'8!+1%,+&,!
spectroscopy
O,$6#')PI.7,)H.I=,)C#=I.2=.1:)
Amplitude-distance
Phase-distance
Raster Spring Imaging
PHC)Q));*#%=+#'3);1,=%I.2=.1:
Magnetic
Force Microscopy
>-'.$#%+.3I-1+:
Electrostatic Force Microscopy
63550&/
Kelvin
Probe Force Microscopy
#45*0
Nanolithography
>-'.2=$,I./,%I:
Force
AFM Nanoindentation
Elastic modulus mapping
;RC Work of adhesion mapping
Distributor Spain & Portugal
IRIDA Ibérica S.L.
C/ Diligencia, 9; 28018 MADRID (SPAIN)
Tel.: +34 91 113 08 24
Fax.: +34 91 011 37 57
e-mail: info@irida.es
www.ntmdt.com, www.ntmdt-tips.com