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BD FACSCanto II
BD FACSCanto II
http://www.bd.com/tw/
Tel : 02-27225660
BD FACSCanto II BD FACSCanto …………… 03
BD FACSCanto Surface Markers……………….. 22
DNA Analysis..…………………. 34
FACSDiva ………………….………… 58
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485
510 (Blue E)
Rhodamine 123
485
546 (Blue E)
BCECF-AM
488
Ratio 520/620
SNARF1-AM
514
Ratio 587/640
Fluo4-AM
Calcium Green-1
488
488
528 (Blue E)
530 (Blue E)
Fura Red
488
660 (Blue B)
H2O2 sensitive
Dihydrorhodamine 123
DCFH-DA
505
505
534 (Blue E)
535 (Blue E)
O2- radical sensitive
Hydroethidine
505
600 (Blue D)
Esterase sensitive
Fluorescein -DA
495
525 (Blue E)
6l±DAÏ
PerCP
1
²³
2
ƒ
„
Viability

‚

‚
h
Ü
´
Mitochondrial
h
Ü

‚
ª

´
‚
ª
pH <
µ
)
58 Optical Configurations
+,
á
ò
?
d
nm %
R
TM
Coherent
Sapphire
÷
3
@
AB
mW %
488C
%
20
FITC, PE, PE-Texas Red,
PerCP, PerCP-Cy5.5,
PE-Cy7, PI
TM
JDS Uniphase
HeNe
633D
%
17
APC, APC-Cy7
Point Source iFlex2000
405¶
%
30
Pacific Blue, AmCyan
Ò
4
+
,
5
6
7
488 Laser
8
9 3
4
5
: ;
<
=
( .
4-2-2 / 0 )
PMT
LP Filter
BP or LP Filters Fluorochromes
Position
nm%
nm%
Blue A:
735
780/60
PE-Cy7
Blue B:
655
670LP
PerCP,
PerCP-Cy5.5
633 Laser
405 Laser
Blue C:
610
Blank filter
N.A
Blue D:
556
585/42
PE
Blue E:
502
530/30
FITC
Blue F:
Blank filter
488/10
SSC
Red A:
735
780/60
APC-Cy7
Red B:
685
Blank filter
N.A
Red C:
Blank filter
660/20
APC
Viiolet A:
502
510/50
AmCyan
Violet B:
Blank filter
450/50
Pacific blue
BD FACSCanto II 59
+
,
6
7
488 Laser
8
9 3
4
5
: ;
<
=
( .
5-3 / 0 )
PMT
LP Filter
BP or LP Filters Fluorochromes
Position
nm%
nm%
Blue A:
735
780/60
PE-Cy7
Blue B:
655
670LP
PerCP,
PerCP-Cy5.5
633 Laser
Blue C:
610
616/23
PE-Texas Red
Blue D:
556
585/42
PE
Blue E:
502
530/30
FITC
Blue F:
Blank filter
488/10
SSC
Red A:
735
780/60
APC-Cy7
Red B:
685
712/21
Alexa Fluor-700
Red C:
Blank filter
660/20
APC
60