ICAR-National Research Centre on Orchids, Pakyong, Sikkim
Transcription
ICAR-National Research Centre on Orchids, Pakyong, Sikkim
National Research Centre for Orchids (Indian Council of Agricultural Research) Pakyong 737106, Sikkim www.nrcorchids.nic.in Printed : July 2015CTO (Production):Kul Bhushan Gupta All Rights Reserved © 2015, Indian Council of Agricultural Research, New Delhi lans'k Hkkjrh; lH;rk Ñf"k fodkl dh ,d vkèkkj jgh gS vkSj vkt Hkh gekjs ns'k esa ,d lqn`<+ Ñf"k O;oLFkk ekStwn gS ftldk jk"Vªh; ldy ?kjsyw mRikn vkSj jkstxkj esa izeq[k ;ksxnku gSA xzkeh.k ;qokvksa dk cM+s iSekus ij] fo'ks"k :i ls 'kgjh {ks=kksa esa izokl gksus ds ckotwn] ns'k dh yxHkx nks&frgkbZ vkcknh ds fy, vkthfodk ds lk/u ds :i esa] izR;{k ;k vizR;{k] Ñf"k dh Hkwfedk esa dksbZ cnyko gksus dh mEehn ugha dh tkrh gSA vr% [kk|] iks"k.k] i;kZoj.k] vkthfodk lqj{kk ds fy, rFkk lekos'kh fodkl gkfly djus ds fy, Ñf"k {ks=k esa LFkk;h fodkl cgqr t:jh gSA fiNys 50 o"kks± ds nkSjku gekjs Ñf"k vuqla/ku }kjk l`ftr dh xbZ izkS|ksfxfd;ksa ls Hkkjrh; Ñf"k esa cnyko vk;k gSA rFkkfi] HkkSfrd :i ls (e`nk] ty] tyok;q)] ck;ksyksftdy :i ls (tSo fofo/rk] gkWLV&ijthoh laca/)] vuqla/ku ,oa f'k{kk esa cnyko ds pyrs rFkk lwpuk] Kku vkSj uhfr ,oa fuos'k (tks Ñf"k mRiknu dks izHkkfor djus okys dkjd gSa) vkt Hkh ,d pqukSrh cus gq, gSaA mRiknu ds ifjos'k esa cnyko ges'kk gh gksrs vk, gSa] ijUrq ftl xfr ls ;g gks jgs gSa] og ,d fpark dk fo"k; gS tks mi;qDr izkS|ksfxdh fodYiksa ds vk/kj ij Ñf"k iz.kkyh dks vkSj vf/d etcwr djus dh ekax djrs gSaA fiNyh izo`fÙk;ksa ls lcd ysrs gq, ge fuf'pr :i ls Hkkoh csgrj Ñf"k ifjn`'; dh dYiuk dj ldrs gSa] ftlds fy, gesa fofHkUu rduhdksa vkSj vkdyuksa ds ekWMyksa dk mi;ksx djuk gksxk rFkk Hkfo"; ds fy, ,d CywfizaV rS;kj djuk gksxkA blesa dksbZ lansg ugha gS fd foKku] izkS|ksfxdh] lwpuk] Kku&tkudkjh] l{ke ekuo lalk/u vkSj fuos'kksa dk c<+rk iz;ksx Hkkoh o`f¼ vkSj fodkl ds izeq[k fu/kZjd gksaxsA bl lanHkZ esa] Hkkjrh; Ñf"k vuqla/ku ifj"kn ds laLFkkuksa ds fy, fotu&2050 dh :ijs[kk rS;kj dh xbZ gSA ;g vk'kk dh tkrh gS fd orZeku vkSj mHkjrs ifjn`'; dk csgrj :i ls fd;k x;k ewY;kadu] ekStwnk u, volj vkSj Ñf"k {ks=k dh LFkk;h o`f¼ vkSj fodkl ds fy, vkxkeh n'kdksa gsrq izklafxd vuqla/ku laca/h eqn~ns rFkk dk;Zuhfrd izQseodZ dkiQh mi;ksxh lkfcr gksaxsA (jk/k eksgu flag) dsUæh; Ñf"k ea=kh] Hkkjr ljdkj Foreword Indian Council of Agricultural Research, since inception in the year 1929, is spearheading national programmes on agricultural research, higher education and frontline extension through a network of Research Institutes, Agricultural Universities, All India Coordinated Research Projects and Krishi Vigyan Kendras to develop and demonstrate new technologies, as also to develop competent human resource for strengthening agriculture in all its dimensions, in the country. The science and technology-led development in agriculture has resulted in manifold enhancement in productivity and production of different crops and commodities to match the pace of growth in food demand. Agricultural production environment, being a dynamic entity, has kept evolving continuously. The present phase of changes being encountered by the agricultural sector, such as reducing availability of quality water, nutrient deficiency in soils, climate change, farm energy availability, loss of biodiversity, emergence of new pest and diseases, fragmentation of farms, rural-urban migration, coupled with new IPRs and trade regulations, are some of the new challenges. These changes impacting agriculture call for a paradigm shift in our research approach. We have to harness the potential of modern science, encourage innovations in technology generation, and provide for an enabling policy and investment support. Some of the critical areas as genomics, molecular breeding, diagnostics and vaccines, nanotechnology, secondary agriculture, farm mechanization, energy, and technology dissemination need to be given priority. Multi-disciplinary and multiinstitutional research will be of paramount importance, given the fact that technology generation is increasingly getting knowledge and capital intensive. Our institutions of agricultural research and education must attain highest levels of excellence in development of technologies and competent human resource to effectively deal with the changing scenario. Vision-2050 document of ICAR-National Research Centre for Orchids (ICAR-NRCO), Sikkim has been prepared, based on a comprehensive assessment of past and present trends in factors that impact agriculture, to visualise scenario 35 years hence, towards scienceled sustainable development of agriculture. v Indian Council of Agricultural Research We are hopeful that in the years ahead, Vision-2050 would prove to be valuable in guiding our efforts in agricultural R&D and also for the young scientists who would shoulder the responsibility to generate farm technologies in future for food, nutrition, livelihood and environmental security of the billion plus population of the country, for all times to come. (S. AYYAPPAN) Secretary, Department of Agricultural Research & Education (DARE) and Director-General, Indian Council of Agricultural Research (ICAR) Krishi Bhavan, Dr Rajendra Prasad Road, New Delhi 110 001 vi Preface The great French mathematician Jules Henri Poincaré once aptly said that “The scientist does not study nature because it is useful to do so. He studies it because he takes pleasure in it, and he takes pleasure in it because it is beautiful. If nature were not beautiful it would not be worth knowing, and life would not be worth living”. That’s why we should care for the mother earth and conserve all her treasures. Biological diversity is one such very important and unique feature of Mother Nature. Biological diversity is the variability among all living organisms existing on earth in various ecosystems and ecological complexes. Orchids are one such beautiful creation of nature and represent royalty and aristocracy in floriculture. They are well known for their uniqueness of shape, breath taking colors, and exceptionally long shelf life that surpasses many other floriculture crops. These qualities have made orchid growing a highly profitable industry all-over the world. A large number of species bear attractive flowers and have capacity for interspecific as well as intergeneric hybridization tha thas generated tremendous possibilities for producing hybrids of diverse floral characteristics. India is one of the primary/secondary centres of orchid biodiversity and the major regions of diversity are Northeastern Himalayas, Western Ghats, and Andaman and Nicobar Islands. Indian orchids have also been used in various indigenous systems of medicines since time immemorial. Whereas, the systematic collection and conservation of orchids for value addition through crossing and selection has started very recently in our country. Today, more than 100,000 hybrids are known globally and cultivated for cut flowers and potted plants and more and more new ones are being registered every month. Orchids have very wide range of distribution in India and have been recorded from low-level plains to an elevation of 4300 m. Orchids are the most evolved family of monocotyledonous plants and is represented by nearly 25,000-35,000 species belonging to 1000 genera. Nearly 1300 species of orchids are found in India distributed in different parts of the country. However, maximum orchid species are found in the Himalayan region especially the North Eastern Himalayas. Nearly 876 orchid species from 151 genera have been reported from this part of the country representing 70% of the orchid flora of India. Many of these endemic and rare species shows high ornamental value. Among the eight north eastern states of India, vii Indian Council of Agricultural Research highest numbers of orchid genera have been reported from Sikkim (137 genera and 525 species) followed by Arunachal Pradesh (126 genera and 550 species) and recognized as paradise of orchids. The orchid index of Sikkim is highest in the country. Keeping in view of the export potential of Orchids, based on the recommendations of the Planning Commission, the Indian Council of Agricultural Research (ICAR) established a National Research Centre for Orchids during VIIIth Five Year Plan at Pakyong, Sikkim since October 1996. In October 1997, the centre also took over the Darjeeling centre of C.P.R.I. Shimla to carry out research on temperate orchids. Since inception, the institute has come a long way to establish itself as a centre of excellence of research on orchids in the country and is undertaking mission oriented research programme on germplasm conservation, crop improvement, crop production and post-harvest management of orchids. The centre has conserved 350 species of orchids collected from tropical to temperate regions of the country at its Pakyong and Darjeeling Campus. Improved production and post-harvest management practices for commercial orchids have been developed. Despite of perennial life cycle and lack of availability of superior breeding stocks in the country, the centre has taken important initiative in crop improvement and few advanced breeding lines have already been registered. Although the initiatives have been taken, still we have to go long way to reach the goals. The global scenario of floriculture trade is changing rapidly. Climate change is creating another new dimension in conservation of natural resources. The institute is also trying to expand its reach to newer areas and climatic zones of the country in order to meet those challenges by opening centres covering the geographical location of the country as well as areas with tropical to temperate climates. What we are today is not only because of the effort of the present generation, but also the vision of our predecessors. The challenges of 2050 are expected to be more complex and diverse in nature and hence we must make ourselves prepared for those challenges as well as opportunities. The Vision 2050 document of the institute is an effort in this direction to envisage those challenges based on present situation and preparing guidelines to face those and excel under that situation. As applied research is current need based, we have focused more on the kind of basic and strategic research to be taken up and prepared a route map to move forward in that direction besides promising to act as centre of excellence for repository of plant genetic resources and knowledge bank of orchids. viii Vision 2050 I express my gratitude and extend my sincere thanks to Dr. S. Ayyappan, Secretary, DARE and Director General, ICAR; Dr. N.K. Krishna Kumar, Deputy Director General (Horticultural Science), ICAR and Dr. T. Janakiram, Assistant Director General (Horticultural Science-I), ICAR New Delhi for their keen interest and provided the much needed guidance in developing the vision document and in bringing it out in its present form. I acknowledge and appreciate the efforts of my colleagues scientists in developing and bringing out this document. D.R. Singh Director ICAR-NRC-Orchids Pakyong, Sikkim ix Contents Message iii Foreword v Preface vii 1.Context 1 2.Challenges 4 3. Operating Environment 8 4. Opportunities 14 5.Goals/Targets 18 6. Way Forward 20 7. Strategic Framework/road map to achieve targets in Vision 2050 22 8.Linkages 25 Context O rchids have become one of the most important segments in world floriculture market. These are grown for cut flowers, pot plant, extraction of biomolecules, herbal medicines. The flowers and pot plant command highest price in markets due to their incredible range of diversity in size, colour, shape, forms, appearance and long lasting flowers. The world export/import trade of orchid cut flowers and plants exceeds $150 million dollars. Of these, about 80% are cut orchids, and the remaining 20% is composed of pot plants. In European Union, the import of orchids accounts for 3% of total fresh cut flower imports with a wholesale value of 21 million Euros. The Netherlands is the largest exporter (39. 67%) of orchids in the world. It is followed by Thailand (28.41%), Taiwan (10%), Singapore (10%) and New Zealand (6%), respectively. The major importing countries include Japan (30%), UK (12%), Italy (10%), France (7%) and the USA (6%). India is the late entrant in orchid business India and vast potential emerge as major orchid producing country in the world due to its suitable climate, availability of manpower and lesser cost of cultivation in comparison to the orchid growing countries. For production of tropical orchids several units have come up in the states of Kerala, Karnataka, Tamil Nadu, Maharashtra, Assam. The units producing temperate orchids particularly Cymbidiums have been setup Sikkim, Meghalaya, Arunachal Pradesh and Darjeeling district of West Bengal. There is a major shift in consumption pattern of flowers tilting towards the exotic flowers like orchids, lilium rose and carnations. It is noticeable in celebrations of birthdays, anniversaries, wedding and other social and official functions. The domestic production is not enough the demands, hence the orchid cut flowers are imported from other countries. The import of cut flowers has risen from Rs. 299.09 lakhs in 2008-09 to 3402.36 lakhs in 201415 (for details see Fig. 1). The export of orchid cut flowers has been very meagre (Fig 2) though there is huge potential for export. Orchids are climate specific crop and require constant monitoring for the management of temperature, light, humidity, air, water and nutritional requirements. So it is necessary to standardize the production technologies to the perfection. Another major input constraint is the non-availability of quality hybrids and planting material in large scale. This necessitates an urgent initiation of a strong breeding programme 1 Indian Council of Agricultural Research based on sound methodologies to develop varieties/ hybrids of internationally accepted quality. Molecular breeding should also be made an integral part of the crop improvement programme to incorporate noble characters in an accurate and rapid fashion. Mass multiplication of quality hybrids or lines through tissue culture is another priority. Standardization of post-harvest management in another major concern and is to be addressed on urgent basis. Research on integrated insectpest and disease management is also a need of hour. A long term proper research support is also necessary to save the valuable genetic resources in a scientific manner, to bridge the gap between beginning of research, 2 Vision 2050 significant output and adoption, to meet up the severe and complex changes in context to climate change, to get benefit from frontier sciences, a vast breakthrough in diversified growth and production by stimulating new ideas and fresh thoughts. Establishment of National Research Centre for Orchids at Pakyong, Sikkim, is in fact a due and timely recognition of the scope and commercial potential of orchid culture in India. Since its inception in 1996, the centre is striving to fulfil knowledge gap required for cultivation and post harvest management of orchids. The centre has developed several elite clones have been developed however, their commercial potential yet to be tested. In vitro propagation protocols of several commercially cultivated orchids are available for large scale propagation of planting materials. The budding orchid industry needs technical know-how for large scale cultivation, improved varieties/hybrids for cultivation, and post-harvest technologies for value addition. In view of the growing demands for organic products, organic cultivation of orchids is another priority area of research. Therefore, four major research programs have been identified to address all issues. The programs will be subject to periodic reviews and modification will be made, if felt necessary in view of any significant change in global trade, commerce and policy matters towards this commodity and its processed products. The institute will seek active collaboration with other research organisations, marketing and banking sectors and extension agencies to establish effective communication channel with the orchid growers and processors for research and effective transfer of technology. q 3 Challenges S pecies of orchids cultivated and exported to different destinations of the world much before the independence. However, the commercial cultivation of orchids began only after the liberalization Indian economy. The North Eastern States and the Costal States of India dominate in the production of orchids. Orchid industry is likely to face several challenges because of changing the global environment, population pressure, and rapid urbanization. To overcome such challenges and to harness the scientific knowledge for improving production, productivity and profitability of the crop are the major concerns of scientists. In 2050, out of 1.6 billion people of our country nearly 50% will live in towns. Orchid flowers worth of Rs. 5000 crore will be needed to meet the domestic demand. Conservation of Orchid Genetic Resource The country has the large genetic base of orchids comprising of 1300 species. Many of them bear large and attractive flowers suitable for cultivation as a potted flowering plant as well parents for hybridization. Several terrestrial and epiphytic species have medicinal properties and used by local practitioners in treating various body ailments. However, these genetic resources are facing are dwindling very fast in their natural habitats due to fragmentation and degradation of natural habitats. One of the major challenges is to conserve and study these dwindling natural resources taking a holistic approach. To address future needs, research will facilitate sustainable use of these genetic resources through (i) characterization, (ii) genetic enhancement and pre- breeding, (iii) functional molecular nanosystems genomics, proteomics, phenomics etc., (iv) gene mining, (v) molecular breeding through tools like marker-aided selection and gene stacking, and (vi) customized genetic engineering (development of trait-specific transgenics). Meeting the Requirement of Market-driven Varieties There are over 1, 50,000 hybrids the world, but only some are suitable these, a few cultivars bred in India and cultivation has yet to be ascertained. 4 are available for cultivation in for commercial cultivation. Of their suitability for commercial India needs to encourage the Vision 2050 cultivation of orchids on the lines of roses on one hand initiate orchid breeding programme using biotechnological and molecular techniques. Increasing Production and Productivity Under Changing Environment Both production and productivity of orchids in the country is low compared with other countries of the world. The shortage of cut flower is met through the imports. The import of orchid cut flowers has increased from 299.09 in 2008-09 to Rs. 3402.36 lakhs in 201415.Thus, production and productivity has to increase through area expansion increasing productivity. According to the projections made by Inter-Governmental Panel on Climate Change global earth temperature will increase by 1.8o to 4.0°C by the turn of this century. It would cause frequent hot extremes, floods, droughts, cyclones, recession of glaciers, lowering the water table, etc. The dynamics of pest and disease of orchids would also likely to alter. In these situations, the production and productivity of these crops has to be increased at reduced cost. It would require determining low-cost-production zones, efficient use of inputs, biotechnological interventions in developing resistant/tolerant varieties, capacity building and global cooperation. Producing Quality Products at Competitive Cost The world over, the competition among flower growing countries is increasing. Simultaneously, the importing countries are imposing new legislation and regulations. Therefore, the flower has to update their operating processes, according to the demand of the market. The distributors and consumers are raising the standards they have set for the quality. Rising cost of inputs and shortage of quality workforce to handle orchid production are posing serious challenges. The efficient use of inputs (water, fertilizers, pesticides etc.) and accurate diseases diagnosis using nano-science, micro-irrigation, re-circulation/re-use of water, farm mechanization will play a major role in tackling these challenges. Meeting the Requirements of Quality Planting Materials Planting should be free from diseases and pests, healthy, vigorous and take the minimum possible time to attain commercial reproductive stage. The planting material of elite clones of orchids is either not available or available in very limited quantity. If available, it is very costly. There is an urgent need to evolve efficient production and delivery mechanism involving (a) production of quality planting material, (b) inspection and monitoring of planting material produced in the laboratories and (c) certification and labelling of planting material. Bioreactors have 5 Indian Council of Agricultural Research been helpful in producing the large number of plants in a limited time. However, the protocols for orchids need to be standardised. Increasing Consciousness for Quality and Meeting Social Responsibility The consumers are becoming more quality conscious because of awareness, media, economic status, easy availability, availability of substitutes, health issues, etc. Consumers want to buy a product with constant quality, price, and added value or a low price. The export supply chain managers want to ensure that products are produced by fulfilling social and environmental responsibility. The producers have to export their produce with Global GAP/Euro GAP, MPS, MPS-SQ certification. These certification systems would be tougher in future. Harnessing Export Potential of Orchids India is bestowed with climatic conditions to produce almost all kinds of orchid at a competitive price. It also has a wide genetic base of orchids to structure and restructure the varieties as per the need of the consumer. In spite, all this export of orchids from India is not encouraging. There are several challenges viz. non- availability of exportable varieties, planting material, infrastructure, inefficient supply chain, cargo space, refrigerated transport, skilled manpower, etc. India need to develop exportable varieties, sound technical know-how in storage and transport through sea routes also to create infrastructural facilities. Preparing Skilled Workforce for Orchid Production Orchids are specialty flowers and require special attention in the cultivation of orchids. Non availability of skilled workforce is one of the major constraints in an expansion of area under cultivation of this crop. The trained workforce should be equipped with the technical know-how to manage these specialised crops. The NRCO has been training the farmers/entrepreneur in the cultivation of orchids. In times ahead, we would require the workforce to produce and market the flowers for export. Diagnosis and Management of Disease and Pest The pest and disease would likely to become a challenge with the changing environment. It would be appropriate to lay emphasis on systematic survey and surveillance pest and disease supported by information technology. The sources of resistance gene would be identified would be used for breeding of resistance varieties. 6 Vision 2050 Post-harvest Management and Transportation The technology and infrastructure play significant role delivery of floricultural products in the hands of the consumer. The cut flowers and pot plants of orchids have relatively longer shelf-life than other flowering plants. However, increasing transport cost and insufficient cargo space would necessitate searching for other alternatives like transportation through sea routes by managing container environment. The research on pre and post-harvest handling, packaging and transportation of orchid flowers as well as potted plants would require a great deal of attention. The shortage of postharvest infrastructural facilities also needs to build up. q Cymbidium Hybrid 7 Operating Environment K eeping in view the export potential of orchids, based on recommendations of the Planning Commission, the Indian Council of Agricultural Research (ICAR) established a National Research Centre for Orchids during VIII th Five Year Plan at Pakyong (Sikkim) in October 1996 in a area of 22.19 acres of land. In October, 1997, the centre also took over the Darjeeling centre of CPRI, Shimla. Since establishment the Centre created infrastructures and facilities for basic and strategic research. With a mission of harnessing science and technology by interfacing research, extension activities for enhancing qualitative productivity, outstanding hybrids and diversified use of orchids, the Centre has mandate to provide single window solution for all aspect of orchids and act as pioneer institute for research and development and a leadership at national arena. The Centre marched ahead to deliver its functions. • To undertake mission orientedprogramme involving basic and strategic research for resolving themajor biotic and abiotic constraints affecting the production, productivityand utilization of orchids. • To act as repository of genetic resources and scientific information on orchids • To demonstrate the improved technologiesand impart training to orchid growers,stakeholders for upgrading knowledge To fulfil this mandate a number of focused research programs were formulated and significant achievements have been made during last 18 years. The centre has already made significant contribution with respect to collection of indigenous and exotic orchid germplasm, in vitromicropropagation and germination of immature embryos obtained after crossing. Various experiments on application of organic and inorganic fertilizer spray were carried out to optimize nutrient requirement and subsequently to reduce the pre-blooming period of Cymbidium orchids. Production technology for bulbous crops and other commercial orchids like Dendrobium, Vanda and Phalaenopsishas been standardized. The perspective plan for National Research Centre for Orchids, Sikkim has been prepared based on the proforma and guidelines supplied by the Council. Under basic information, mandate, budget, manpower, salient research achievements by the centre have been 8 Vision 2050 summarized. The national scenario of flower crops in terms of strengths, weaknesses, opportunities and threats have been analyzed. Tremendous yield potential of the mandate crop, viz. orchid, availability of diverse types of germplasm, adequate market potential including export of cut flowers, high market price, cheap labour and climatic advantage for raising the crop, OGL policy of the government are few strengths of the flower production. However, high cost of planting materials, inadequate packaging and transportation facilities, poor infrastructure, poor marketing facilities etc. are some of the weak links. Nevertheless, there is tremendous scope to increase productivity, generation of employment, support to the industry and export of cut flowers, potted plants and planting materials. In order to undertake various research and development programme the following thrust areas have been identified. 1. Collection, maintenance, documentation and conservation of germplasm. 2. Morphological and molecular characterization of important germplasm to safeguard valuable indigenous generic resources. 3. Strategic development of new improved varieties with genetic potential,hybrids with high yield, quality and tolerant to biotic and abiotic stresses. 4. Development of protocol for mass multiplication of improved varieties/hybrids. 5. Development of production technology for increasing productivity of new hybrids/varieties of commercial orchids. 6. Standardization of pre and post-harvest techniques for reducing the storage and transport losses and development of value added products. 7. Promotion of integrated insect-pestand disease management programme. 8. Dissemination of technologies through vibrant training programme, demonstration, publication and mass media. 9. Preparation of data base of all indigenous orchids to strengthening the floricultural research information system with special reference to orchids. 10.Promoting the human resource development on relevant discipline. Salient Achievements Crop Improvement • Total 3650 number of accessions of orchid in 350 species collected at ICAR-NRCO, Pakyong and Darjeeling Campus from different parts of the country. 9 Indian Council of Agricultural Research • Genetic diversity of Cymbidium (10 species) studied using RAPD markers. • Molecular analysis of 59 Dendrobium species was done using STM markers. • Complete genetic diversity analysis of Indian Vanda (18 species) with RAPD, ISSR, SSR, STM and phylogenetic markers. • The DUS test guidelines of Cymbidium, Dendrobium,Vanda, Cattleya, Phalaenopsis and Oncidium were developed and these have been notified for registration under Protection of Plant Variety & Farmers Right Authority Act. • Protocol for multiplication of PLBs of Cymbidium orchids standardized • Three (3) Cymbidium and four (4) Paphiopedilum hybrids identified. •Two Cymbidium hybrids ‘Darjeeling Nymph’ and ‘Darjeeling Delight’ registered through Royal Horticultural Society (RHS), London. • Two (2) in-vitro clones of Paphiopedilum venustum and oneex-vitro clone of Cattleya maxima, three variants of Coelogyne nitida, one variant each of Calanthepuberula, Eriaspicata and Liparis bootanensis identified. • NRCO –Coll-77 of Red Vanda and NRCO-42 (Dendrobium) registered through PGRC Crop Production • Technologies developed for regeneration of plantlets through backbulbs in Cymbidium orchids. • Techniques for preparation of slow release organic nutrition for orchids developed. • Suitable cut-flower and potted plant hybrids of Cymbidium, Dendrobium, Vanda, Phalaenopsis, Oncidium,Cattleya, Mokara and Aranda orchids identified for commercial cultivation. • Cultural practices of commercial orchids like Cymbidium, Dendrobium, Vanda, Oncidium, Cattleya and Phalaenopsis standardized. • Technologies developed for round the year production of cut flowers of Dendrobium and Phalaenopsis through selection of various types of hybrids. • A complete protocol of Post-harvest technology of Cymbidium orchids developed starting from harvesting stages, pulsing, impregnation, bud opening, vase solutions and packaging. •In Phalaenopsis, flowering was induced kept under day temperature 25°C ±2°C and night temperature 17°C ±2°C. • About 50,000 plantlets of Cymbidium, Zygopetalum and Cattleya hybrids produced using tissue culture techniques. 10 Vision 2050 Crop Protection • Cym. Mosaic Virus (CyMV) and Odontoglossum Ring spot Virus (ORSV) were reported in most of orchid species. • Viral indexing of 30 Cymbidium hybrids and 3 Dendrobium hybrids done by ELISA technique. • RT-PCR of ORSV standardized and used for detection of ORSV from planting material. • Metalaxyl (0.1%) identified as effective to control black tot disease of Cymbidium. • Application of Mancozeb (200-500 ppm) + Carbendazim (200-500 ppm) found effective against anthracnose of Cattleya and Phalaenopsis orchids. • Ciprofloxacin hydrochloride (400 ppm) and tetracycline hydrochloride (1000 ppm) found sensitive against soft rot bacterium of Cymbidium orchids. • Trichoderma viridae showed antagonistic in vitro against orchid anthracnose. • Molecular characterization of orchid anthracnose done by PCR assay • Neem oil 0.03 EC found effective against red spider mite and aphid on Cymbidium • IPM module M-5 (tobacco extract 5%, neem oil 0.03% EC 5ml/l and bifenthrin 10EC 0.25%) showed effective against mite on Cymbidium. • First report on diaspid scale insect, Lepidosaphes pinneformis, aphid, Aulacorthum circumflexum on Cymbidium and Long tailed mealy bug, Pseudococcus longispinus on Dendrobium. • New report of an aphelinid wasp, Coccophagus ceroplastae, Aphytis spp. As parasitoids. Demand Driven Technologies and Products Technologies already commercialized • Micropropagation of Cymbidium orchids Technologies absolutely ready for commercialization •In-vitro protocol development of Cymbidium ‘Golden Girl’ and Cymbidium ‘Lunavian Atlas’, Cymbidium ‘Soul Hunt-I’, Cymbidium ‘Sleeping Nymph’ and Cymbidium ‘Pine Clash Moon Venus’ and Zygopetalum intermedium. • DUS Test guidelines of Cymbidium, Dendrobium,Vanda, Phalaenopsis, Cattleya and Oncidium orchids 11 Indian Council of Agricultural Research • Innovative method for propagation of Cymbidium through back bulbs • Post-harvest technology of Cymbidium orchids Technologies in the pipe line • A rapid and efficient method for high quality DNA from leaves of Orchids. • Breeding stocks/clones • NRCO-Epidendrum hybrid/2005-01: (Epidendrum radicans x Epidendrum xanthinum) • NRCO-Pl x Pw hybrid/2007: (Paphiopedilum lawrenceanum x P ‘Winstine Churchill’) • NRCO-42/2007 (IC0566525; INGR10073): (Dendronium ‘Emma White’ x D ‘Pompadour’) • NRCO-Cymbidium hybrid-HxB/2008-01: (Cymbidium lowianum x Cymbidium ‘Show Girl’) • NRCO-Cymbidium hybrid-BxH/2008-01: (Cymbidium ‘Show Girl’ x Cymbidium lowianum) • NRCO-Paphiopedilum clone-01/2008: EDV from P venustum species (mutant) • NRCO-Paphiopedilum clone-02/2008: EDV from P venustum species (mutant) • NRCO-Inter Generic Hybrid/2010: (Arachnanthe cathcarthii x Vanda coerulea) • PBX-05-55/2011-01: (Cymbidium ‘Golden Girl’ x Cymbidium ‘Red Star’) • PBX-05-34/2012-01: (Cymbidium ’Red Star’ x Cymbidium ’Fancy Free’) • PBX-05-56/2012-01: (Cymbidium lowianum x Cymbidium tigrinum) • Package of practices for production of Cymbidium orchids • Package of practices for production of Dendrobium orchids Potential Commercial Products • Slow release organic fertilizer for orchids • Dried orchid species and hybrids • Herbarium kits for valuable species Services • Providing technical support to private sector laboratories for multiplication and expansion of orchid cultivation • Human resource development through MTC, Short Course Training, On/Off Campus Training 12 Vision 2050 • Evaluation of floriculture related research projects • Preparation of course curriculum and question papers on floriculture in graduate and post-graduate levels. • Evaluation of Thesis on floriculture in post-graduate levels q Cymbidium nursery (Ex-vitro hardened) 13 Opportunities T he National Research Centre for Orchids will strive to harness power of science in enhancing the potential of orchid genetic resources, developing cultivar cultivars, increase production and productivity of orchids, enhancing input use efficiency, reducing cost and post-harvest losses, improving quality cut flowers and pot plants through new scientific tools. The delivery of technology is becoming more complex than ever before due to increasing quality consciousness and increasing the cost of inputs. Incidentally, new tools, methods, techniques and approaches are emerging very fast to keep the promises of technological breakthroughs to fulfil the goals. Genetic-resource Enhancement Genetic resources are primary drivers in augmenting productivity, enhancing quality and meeting the challenges of biotic and abiotic stresses in crop plants in the lesser time, cost and space. India is bestowed with 1300 species orchids that account for nearly 7 percent of world’s orchid genetic resource. These are useful structuring new cultivars and restructuring the existing ones for the desired traits. ICAR- NRCO is the National Active Germplasm Site for Orchids and holds about 3500 accessions belonging to 350 species. There are plans to enrich germplasm collection by collection and introductions. To address future needs, research will focus use of genetic resources through characterization, genetic enhancement, clonal selection and pre-breeding. The centre would also make use of molecular and biotechnological tool in breeding of cultivars. Diversification for Value Addition In India, presently Cymbidium and Dendrobium are grown commercially for cut flower production. There are a large number of genera like Vanda, Aranda, Mokara, Arachinis, Ascocenda, Paphiopedilum, Cypripedium, Oncidium, Odontoglossum, Zygopetalum, etc. can be grown for cut flower as well pot plant production. The orchid business encompasses cut flowers, loose flowers and value added products like garland, dried flowers, bouquet and corsage and pot plants. Further, many species of orchids are edible have high medicinal 14 Vision 2050 value and pleasant aroma that can be explored for value added products like nutrient supplements, herbal medicines and other cost competitive various products like soap, incense stick, perfume oil, dry flowers, packed items etc. The live and dried orchid plants and roots have a high demand for medicinal uses. Therefore, to meet the demand for these commodities, research focus would be further directed to augment their production more efficiently and competitively. Management of Natural Resources to Improve Production Integrated nutrient management incorporating the judicious combination of organics, bio fertilizers and nutrient to mitigate different abiotic stresses is needed for the crop. Seasonal changes in climatic conditions have an impact on orchid productivity through observable changes in terms of phenological events, such as flowering time, floral variation, harvest and finally the production. Nutrient and irrigation schedule, therefore, needs to be standardised under such situations. Phenomics and precision cultural practices using the advances in remote sensing and GIS technology has to be implemented to meet these targets. New Technologies In recent years, biotechnology has created remarkable opportunities and has become the world’s fastest growing and the most rapidly changing technology. Biotechnology has a considerable potential to address many of the challenges in orchids. Various biotechnological tools like automated bio screening, genetic engineering, production of photosynthetically efficient plants, production of biopesticides and bio fertilisers, application of MAS to speed up the breeding for important traits and many others. Genomics techniques are advancing at a rapid pace and expected that techniques for sequencing of the individual genome will become affordable very soon. The genome analysis will allow allele mining for different traits and global expression analysis by RNA sequencing will allow elucidation of gene function and study of the effect of different conditions at cellular level itself. Such analysis will be utilized for developing varieties with desirable traits and good quality. Different proteomics approaches will allow understanding of host–pathogen interaction for major diseases also and help in developing breeding as well as management strategies. Nanotechnology will play an important role in pest control. Pesticide formulation based on nanotechnology will be used for effective pest control and ensure the safety of the crop. Biocontrol methods for disease and insect pest control would provide opportunities to grow plants with minimal pesticide use. 15 Indian Council of Agricultural Research Automation in large-scale micropropagation of quality planting materials is best achieved by adopting bioreactor. Adopting mobile technology for market data terminals as front-end trading solutions, trading gateways, and data servers to meet the specific needs of the trading environment is another aspect. Bio-risk Management Bio-risk is increasing due to climate change which adding cost, reducing production and is adversely affecting the orchid culture. The efforts would be made to develop effective and integrated risk management production systems and institutional mechanisms to overcome the problem of bio-risk. Bio-risk intelligent systems like early warning system, migratory movement of bio-risk agents would be developed with the help of different information and communication technologies (ICT). It would help in taking an informed decision at the local, regional and national level. Development of rapid and reliable diagnostics against pests and pathogens including invasive species and management of new invasive pests and pathogens will help in Bio risk management.DNA barcoding and sequencing techniques will be used for monitoring and minimizing biosecurity elements. Orchid Cultivation in Degraded Soils and Non-traditional Areas As per estimates nearly 120.72 million ha of land in the country is degraded due to soil erosion and about 8.4 million ha has soil salinity and water-logging excessive mining of micronutrients, leading to deficiency of macro- and micro- nutrients in the soils. Since, orchids are grown on artificial media they can be grown on degraded soil provided climate is suitable and other inputs are available. The efforts would be made to develop production technology for cultivation of orchids in non-traditional areas of the country. Technology Transfer Systems For any improvement programme, it is important to strive continuously to develop new and better technologies. Effective delivery mechanism of the new developed technologies would greatly help in reducing the gap between the potential and the realized productivity. More far-reaching, participatory information and communications technology would be evolved by optimizing print and delivery systems and by showcasing research products for effectively linking research accomplishments with the stakeholders. The public-private partnership will also be explored both for technology development and their transfer. 16 Vision 2050 ICAR hub meeting/Agribusiness campaign inviting state extension department and SAU would also be regularly organized to transfer the technology to the beneficiaries. New ICT tools will also be utilized for effective technology dissemination. Human Resource Development Enhancing the quality of human resource is a pre-requisite for implementing and upgrading the research programmes, development of new advanced technologies, evolving institutional arrangements to face challenges and harness the opportunities. Maintaining global standards and enhancing competitiveness are equally important in agri-business and technology development. Efforts will be, therefore, made to enhance competence and develop state-of-the-art infrastructure. q Cymbidium hybrid 17 Goals/Targets I ncreasing the production and productivity of different kinds of orchids for meeting domestic demands, curtailing imports and earnings through export are the primary goal for next four decades. The other important goals are to maintain the productivity levels under adverse conditions and diversify for value addition and consumer preference. Increasing production and productivity of orchids by bringing more and more area under scientific cultivation of improved varieties established in trade. To meet the shortage of quality planting materials the young entrepreneurs would be motivated and trained in production of planting materials through tissue culture. The cultivars would be systematically bred for national and international markets using conventional, biotechnological and molecular tools and production and propagation technology would be developed before the release of the cultivar/varieties. For maintaining sustained level of production and productivity under adverse conditions, IT-based decision support systems for risk warning and contingency planning would be developed and used. To meet the scarcity of water in changing climate scenario, the technologies for efficient use of water would be developed. The technologies would aim at reducing transpirational losses, quantified targeted delivery and recirculation of water. The understanding physiological response of orchids to biotic and abiotic stresses and processes, interactions and components of the orchid ecosystem will help in mitigating both biotic and abiotic stresses and realizing the production potential. Improving resource use efficiency to increase benefit: cost ratio is another goal as conventional viticulture system of India involves high input consumption in terms of electricity, labour, etc. Postharvest handling and economical and efficient modes for the transportation of orchid products for domestic as well as export should be ascertained would be worked out for a safe delivery of the product. With changing level of awareness, the consumer would like buy high grade superior quality flowers in appealing packs. Therefore, reduction in post-harvest losses by means of maintaining cold chains and proper handling of the produce will contribute to fulfil the targets. Therefore, development of technologies for the production of quality flowers and 18 Vision 2050 other value-added processed products will be another goal Orchid flowers are perishable commodity and the losses after harvesting are significant. To meet social and environmental responsibility in production and marketing of orchids the use of safe pesticides and fungicides are to be minimized to safest level. The research efforts would be directed to develop organic production protocols by using bio-fertilizers, biopesticides etc. q Cymbidium hybrid 19 Way Forward T he world export/import trade of orchid cut flowers and plants exceeds $150 million dollars. Of these, about 80% are cut orchids, and the remaining 20% is composed of pot plants. Orchids alone account for 3% of total fresh cut flower imports to the European Union, with a wholesale value of 21 million Euros. Since Japan is the world’s largest importer of cut orchids, Asia dominates the world trade. The main suppliers are Thailand, Singapore and Malaysia; Holland, however, reported 240 ha of Cymbidium in 2001 and Italy, France and New Zealand hold productive sectors valued at over one-half a million US dollars. The orchids have taken a significant position in cut flower industry due to its attractiveness, long shelf life, high productivity, the right season of bloom, easy in packing and transportation. Orchid accounts for a large share of global floriculture trade both as cut flowers and as potted plants and is estimated around 10% of the international freshcut flower trade. The value of fresh cut orchids and buds trade during 2007-2012 with the average trade value was US $ 483 million. In 2012, there are more than 40 exporting orchid countries and 60 importing orchid countries around the world, and the total size of the global trade is US $ 504 million. The Netherlands is the top most exporter (39. 67%) of orchids in the world. It is followed by Thailand (28.41%), Taiwan (10%), Singapore (10%) and New Zealand (6%), respectively. Importing countries are mainly Japan (30%), UK (12%), Italy (10%), France (7%) and the USA (6%), respectively. The total orchid cut flower trade of the world mostly consists of 85% Dendrobium species and 15% Phalaenopsis and Cymbidium species and Asia is the main source of orchid to enter the world. Research programs were outlined for next 35 years (Annexure 1) have been formulated to achieve the targets/goal and meet the challenges. Climate resilient orchid culture will be the prime focus as climate change is expected to be the main factor with multidimensional effects on orchid cultivation. Dwindling natural resources like land and water other resources like nutrients and energy warrants its judicious and efficient use and in turn this will help in conservation of resources and also enhance input use 20 Vision 2050 efficiency. Precision orchid culture, therefore, is to be given emphasis in coming years. Advantage will be made of other branches of science viz. space, engineering and communication technology for improving the orchid industry. Plant health management is pivotal to the sustained production and resource conservation and thus forms an integral component result of their improved socio-economic status, necessitates enough attention to value addition and new generation products. Being an economically important crop, orchids offers a good opportunity to tap and expand new value added products .Given difficulty in the timely availability of skilled and unskilled labour in sufficient number for various field operations cultivation has also been proposed in research programs. Advanced and communicative technology would be employed for dissemination of knowledge and technologies. Risk analysis and disaster management market analysis would be done to support the orchid growers of the country. The institute aimed to develop the capabilities to act as Centre of Excellence in 2050. Effective implementation of all research programs will require a good support from the government in terms of liberal funding for development of infrastructure for research. Project based budgeting and flexibility and autonomy in procurement procedures will further help in achieving targets on time. Strengthening of national and international linkages and active participation of orchid stakeholders and supporting industry will also be sought to address different issues affecting large scale commercialization in the country. The institute will continue to strive to play a lead role among different stakeholders for the progress of orchid industry in the country, especially in NEH region. q 21 Strategic Framework/Road Map to Achieve Targets in Vision 2050 Annexure - 1 Goal Conservation and Evaluation of genetic resources Precision Orchid culture Approach Performance Measure Period Multidisciplinary research Collection, characterization and cataloguing of o f I n t e g r a t e d o r c h i d 850 indigenous germplasm from NEH Region, conservation approach 288 species of North-Western Himalayas, 379 species of Peninsular India and 115 species of Andaman & Nicobar Islands. Conservation genetics using molecular methods and phylogenetic studies. 2025 Mycorrhizal associations with analysis of DNA sequences. 2030 Pollinator interactions with population genetics and phylogenetic analysis of orchids and pollinators. 2030 In situ conservations (Biosphere Reserves, National Parks, Sacred Grooves, Gene Sanctuary and Individual Trees). 2025 Ex situ conservations (Field gene banks, Botanical garden, Herbal Garden, In vitroconservation, Cryo-preservation and DNAconservation-DNA Bank). 2035 Genetic engineering and transformation for early flowering,colour as per consumer’s preference and extending shelf-life in commercial orchids Development of Bar codes for germplasm. 2025 Technologydevelopment Development of new hybrids/varieties suitable f o r i m p r o v e d a n d n e e d for different orchid growing zone and improved basedresourceutilization input use efficiency. 2040 Development of technologies for improved Input use efficiency (potting media,fertilizers, water, bio regulators, pesticides,fungicides etc.). 2030 Delivery systems (nanotechnology, drippers, sprayers etc.). Disease/pest forecasting. 2040 Use of Information and Communication Technology. 2030 Sensor based robotics in production management. 2040 22 Vision 2050 Increasing productivity of orchids through production of quality planting materials and protected cultivation Targeting the production levels by propagating and distributing quality planting materials of improved hybrids Production of nucleus planting materials of new hybrids/varieties of commercial orchids through mass multiplication using standardized protocols. 2030 Developing technologies suitable for protected cultivation of commercial orchids. 2035 Climate Resilient for commercial orchid cultivation Generation of eco-region Development, evaluation and identification of specific technologies based suitable hybrids/varieties resilient to climate on maximum productivity of change. available natural resources Functional genomics of biotic and abiotic stress response. 2030 Decision support system/s for sustained orchid production under abiotic and biotic stress. 2035 Cost effective nutrient and agro-climatic management through optimization for quality flower production. 2030 Development and popularization of cost effective agricultural practices (INM/IPM) for increasing productivity. 2030 Quantification of water use efficiency and water requirements in orchids. 2030 Carbon sequestration potential in orchid based cropping systems. 2040 Development of profitable orchid based cropping systems with other high value flower crops. 2025 2035 Reducing pre-blooming period of Cymbidium orchids using effective management practices for higher income. Management of biotic stress to reduce production losses by developing diagnostics, forecasting models, biocontrol systems and effective management strategies Identification of new and effective bio-molecules for management of biotic stresses coupled with development of innovative diagnostic techniques for rapid, accurate and cost effective detection of high impact pests and diseases. Breeding for disease resistant varieties. 2050 Identification and management of nutrient disorders. 2030 Identification and management of physiological disorders. 2030 Integrated Pest Management and Integrated Disease Management. 2035 Surveillance, identification and characterization of new invasive pests and pathogens, pest risk analysis. 2035 Characterization of rhizosphere microbial community structure and effect of engineered nano-particles on microorganisms in the rhizosphere and non-rhizosphere. 2040 Development of rapid and reliable diagnostics against pests and pathogens including invasive species. 2045 23 Indian Council of Agricultural Research Management of new invasive pests and pathogens. Pre and Post-harvest management, value addition and new generation products Product diversification for Breeding and evaluation of varieties for targeted improved marketability and value added products like dry flowers, smart price realisation packing, perfume etc. 2040 Improved technologies for the production of processed products 2030 Development of pre-harvest, harvest and postharvest technologies for specific target groups like domestic and export market and hybrid/ variety specific technologies. 2040 Development of packaging for marketing of commercially important fresh and dried produce. 2035 Development of orchid based technologies for dry flowers and floral arrangement. 2040 Use of orchid and waste for production of phytochemicals such as colour, food, feed, herbal medicines and essential oils. 2050 Patenting technologies related to species. Technology dissemination Participatory approach Large scale field demonstration of production for effective technology technologies using advanced tools. dissemination Grower’s seminars, KisanMela, exhibition, Brainstorming session, Web/video 2035 2030 conferencing and other ICT tools. 2035 Inter–institutional collaboration to facilitate popularization of viable technologies. 2030 On-farm demonstration of new technologies. 2040 Commercialization of technologies and patenting. Documentation and utilisation of ITK’s. 2045 q 24 Linkages Local Institutes The centre has already built up a strong linkage with Botanical Survey of India, G.B. Pant Institute of Himalayan Ecology and Environment and Horticulture and Cash Crop Department (HCCD), Govt. of Sikkim. The linkages with following national and international institutions are being proposed. National Research Institutions Sl. No. Area of collaboration/support Institutions 1. Collection and conservation of orchid genetic resources National Bureau of Plant Genetic Resources, New Delhi 2. DNA profiling and bar coding of orchid germplasm National Research centre on DNA Fingerprinting 3. Use of GIS in collection and conservation of orchid biodiversity Indian Institute of Spice Research (IISR), Calicut 4. Post harvest management of tropical orchids College of Horticulture, KAU, Thrissur, Kerala 5. Authentication of orchid species Jawaharlal Nehru Tropical Botanical Garden and Research Institute (JNTBGRI) 6. Virus indexing National Chemical Laboratory, Pune , Maharashtra Institute of Himaayan Bioresource Technology, Palampur, Himachal Pradesh 7. Integrated Pest Management National Centre for Integrated Pest Management, New Delhi Project Directorate of Biological Control (PDBC), Bangalore Indian Agricultural Research Institute (IARI), New Delhi 8. Packaging and post harvest technology Central Institute of Post Harvest Engineering & Technology (CIPHET), Ludhiana, Punjab 9. Training on PCR, cloning of Virus genes and production of polyclonal antibodies of major orchid viruses Indian Agricultural Research Institute (IARI), New Delhi Linkages with Other Institutions The Centre has linkage with Department of Agriculture and Cooperation, Ministry of Agriculture, New Delhi, Assam Agricultural 25 Indian Council of Agricultural Research University, Jorhat, Central Agricultural University, Imphal and Gangtok, Sher-e-Kashmir University of Agriculture and Technology, Srinagar, J& K, Central Potato Research Institute, Shimla, Y S Parmar University of Horticulture and Forestry, Solan, Indian Institute of Horticulture Research, Bangalore, Central Institute of Tropical Horticulture, Lucknow, Vivekananda Parvatiya Krishi Anusandhanshala, Almora, ICAR Research Complex for NEH Region, Barapani, Central Plantation Crop Research Institute, Kahikuchi. Linkages with International Institutions Sl. No. 1. Area of collaboration/support Institutions Genetic conservation University of Adelaide, Australia 2. Mycorrhizal association University of Western Australia, Perth 3. Genetic conservation, breeding and climate change Kew Botanical Garden, London 4. Orchid breeding and Microbiolog University of Hawaii, Honululu 5. Climate Management and protected cultivation Texas A&M University, USA 5. Micro propagation using bio reactor technology University of Seoul, Taiwan 6. Orchid Research Centre, National Cheng Kung University, Tainan, Orchid Biology and Biotechnology Coordination The overall programme will be coordinated under the leadership of Director, ICAR-National Research Centre for Orchids. The Director will be responsible for national and international network, coordination on special projects like ICAR, CSIR, DST etc. Executive Arrangements The execution of programme will be effected by the Director, ICAR-NRCO as per mandate and priorities through suggestions and comments of QRT, Institute Management Committee, Research Advisory Committee and Institute Research Advisory Committee. Public and Private Partnership Growers–State-ICAR partnership has been created by developing of model floriculture units at different locations of Sikkim. Status of NRCO by 2050 The National Research Centre for Orchids should be upgraded to an “Institute of National Importance” with more number of scientific personnel’s and sophisticated tools and equipments. The centre’s 26 Vision 2050 intervention for precision farming of large as well as small scale production, development of demand driven hybrids and smart packaging will reflect the presence of the institute at national and international level. q 27 Indian Council of Agricultural Research Fig. 1 Conservation of genetic resources 28 Vision 2050 Fig. 2 Varietal development with resistance to biotic/abiotic stress 29 Indian Council of Agricultural Research Fig. 3 Resource budgeting and cropping system management 30 Vision 2050 Fig. 4 Bio-risk Management 31 Indian Council of Agricultural Research Fig. 5 Post-harvest management & value addition 32 Vision 2050 Fig. 6 Production processes in orchid industry 33 Indian Council of Agricultural Research Fig. 7 Technology transfer and impact Assessment 34 NOTES NOTES Laser typeset at M/s Print-O-World, 2568, Shadipur, New Delhi 110 008 and printed at M/s Royal Offset Printers, A-89/1, Naraina Industrial Area, Phase-I, New Delhi 110 028.