Supplementary Data for Hong et al. article

Journal of Alzheimer’s Disease 22 (2010) 1–3
IOS Press
1
Supplementary Data
FK506 Reduces Amyloid Plaque Burden and
Induces MMP-9 in Aβ PP/PS1 Double
Transgenic Mice
Hyun-Seok Honga,b,1, Ji-Yeon Hwanga,1, Sung-Min Sona , Yoon-Hee Kima , Minho Moona and
Mook-Jung Inheea,∗
a
Department of Biochemistry and Biomedical Sciences, Seoul National University College of Medicine, Seoul,
Korea
b
Institute on Aging, Seoul National University College of Medicine, Seoul, Korea
Accepted 1 June 2010
Supplementary Figure S1. Mouse body weight monitoring during experimental period. Mice bodyweights were measured once a week. There
was no significant body weight change during experimental periods (7 weeks) in AβPP/PS mice (left panels), wild type littermate mice (right
panels), vehicle (upper panels) or FK506-injected mice (lower panels).
1 Authors
contributed equally.
Correspondence to: Mook-Jung Inhee, PhD, Department of Biochemistry and Biomedical Sciences, Seoul National University College of Medicine, 28 Yungun-dong, Jongro-gu, Seoul, 110-799, Korea. Tel.: +82 2 740 8245; Fax: +82 2 3672 7352; E-mail: inhee@
snu.ac.kr.
ISSN 1387-2877/10/$27.50  2010 – IOS Press and the authors. All rights reserved
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H.-S. Hong et al. / FK506 Reduces Amyloid Plaques
Supplementary Figure S2. Aβ1−40 levels in FK506-injected mouse brains. Human Aβ1−40 levels are decreased in FK506 treated mice brain,
although not reaching statistical significance (Veh; Vehicle injected, FK506; FK506 injected).
Supplementary Figure S3. AβPP/PS mice brains exhibit amyloid plaques and GFAP positive astrocytes (middle and right panels). But both of
amyloid plaques (green, arrow) and GFAP-positive Immunostaining analysis of Aβ and GFAP. astrocytes (red) were reduced in FK506 injected
mouse brains (right panel) compared to vehicle injected mice (middle panel). Wild type litter mate mouse brain showed no amyloid plaque
staining (left panel).
H.-S. Hong et al. / FK506 Reduces Amyloid Plaques
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Supplementary Figure S4. Correlation analysis among CaN activity, MMP-9, synaptophysin, PSD-95, GFAP, and plaque burden. There is
significant correlation between GFAP and amyloid load. For MMP-9 level and amyloid load, it shows a tendency to negative correlation, even
though it is not statistically significant. We did several correlation analyses for various pairs. Briefly, when calcineurin activity was lowered by
FK506, MMP-9, synaptophysin, PSD-95 levels were increased, while GFAP level and plaque burdens were decreased when CaN activity was
low.