the ViewLux imager

P R O D U C T
N O T E
Multimode Detection
Key Features
• Unrivalled throughput of up to 200,000
samples per hour under continuous
operation
• N
ext generation, low-maintenance, Peltiercooled CCD camera
• Supports 5 key non-radiometric technologies
and a wide variety of radiometric assay
types
• Robotic loading and/or plate stacker options
• Measures any sample in a microplate format
or footprint (e.g. gels, blot membranes and
slides)
ViewLux uHTS
Microplate Imager
Introduction
A
B
The ViewLux® is the gold standard, ultra highspeed microplate imager, ideal for high throughput
screening of compound libraries requiring the
ultimate in speed, reliability and high quality data.
In fact, over 150 ViewLux systems have been installed in all major pharmaceutical
and many biotech companies worldwide.
The ViewLux system features telecentric lens technology which maximizes light
collection from the sample plate and increases sensitivity while decreasing parallax
error that can result from wells being at different distances from the lens.
Because the ViewLux imager generates
both image and numerical data, it provides
users with additional insight into their biology.
Standard camera lens
Telecentric lens
A telecentric lens has no parallax error
and no loss of photons due to geometry
and wall shading.
The ViewLux imager now features the next
generation of low-maintenance Peltier-cooled,
CCD camera technology that eliminates the
need for a cryogenic cooling system. The
camera is designed for more sensitive
detection, maximum reliability and easy
maintenance.
The ViewLux imager provides similar
performance to a PMT based reader (A), and
its imaging capability delivers additional visual
data (B) for assay development applications.
Choose the best detection for your assay
Latest generation camera system
The system performs luminescence, fluorescence, time-resolved
fluorescence, absorbance and fluorescence polarization which
makes it perfectly suited to a wide variety of today’s fastest
non-radioactive and radioactive assay technologies. The system
shows excellent correlation between results from 384- and
1536-well plates, allowing easy assay miniaturization, and with
the ability to read a 1536-well plate in 30 seconds, the system
can manage throughputs in excess of 200,000 samples per
hour under continuous operation. The ViewLux imager can also
handle plates in a 3456-well format.
• Fast cooling with easy switch off when not in use – ready
to use in 30 minutes after restart
Time-Resolved
Fluorescence
• Compact – no separate camera controller, the cooling
system is integrated into the instrument
• Easy maintenance – no vacuum pumping, no heated
tubes
• Lower background than ever before – more sensitive
detection limit
• Comparable in performance to previous generations of
ViewLux readers for maximum confidence and transferability
Radiometric capability
Luminescence
The ViewLux uHTS Microplate Imager can also read a variety
of homogeneous radiometric assays such as Scintillation
Proximity Assays (with red-shifted SPA beads), Image
FlashPlates™, ADME Tox and environmental FATE analysis
of HPLC fractions in LumaPlates™.
Fluorescence
Intensity
Work the same way with all assay types
The 3 graphs above show the close correlation in performance between
384- and 1536-well for TRF, luminescence and fluorescence technologies
on the ViewLux imager, allowing confident assay miniaturization.
Dual luminescence
The ViewLux uHTS Microplate Imager excels in high
throughput, dual luminescence experiments, particularly
those requiring only a single reagent such as Chroma-Luc™
(Promega Corporation). Its CCD camera design delivers
simultaneous measurement of 1536 wells at each wavelength, so avoiding the signal decay associated with PMT
based readers due to measurement time across the plate.
The ViewLux imager can read all commonly used non-radiometric labels. To change from one technology to another,
simply place the appropriate barcode on the microplate and
the ViewLux system will read it, whether it is on the long
or short edge of the plate. With the one imaging platform
you can work in the same way with all your labels and assay
types, reducing learning curves, saving space and maximizing
the use of robotic automation systems.
A ViewLux system designed for the way you work
A modular design means that the ViewLux imager can keep
up with changes in your application or workflow without
major reinvestment.
Options include:
• Single or double 32-shelf stacker
• External platform for robotic loading
• Combination 32-shelf stacker and external platform for
robotic loading
2
Reading time with the ViewLux
imager: 4 mins/384-well plate.
Reading time with the TopCount
system: 6 hrs/384-well plate.
CASE STUDY: ViewLux Imager vs TopCount for 14C Radioisotope
Detection in Biological Samples
An example of how a lower throughput application can be improved using the
ViewLux system is shown above. The TopCount™ plate-based scintillation counter
is commonly used to generate a chromatogram from HPLC fractions of separated
metabolites. While highly sensitive in comparison to the usual method of radio-HPLC
detection, the read time in this case is 6 hours. Using the ViewLux imager, the
same result is achieved in 4 minutes with minimal loss of signal to background.
Specifications
Power requirements (instrument, PC and cooler)
Standby: 1200 VA
Running: 1800 VA
Maximum: 2000 VA
Power requirement for the instrument only: 1000 VA
Mains power supply: 100-230 VAC, 50/60 Hz, nominal
(operating range 90-253 V)
ViewLux fuses: 2 x T8 A H 250 V, for mains voltages of 220-230 V
2 x T15 A H 250 V, for mains voltages of 100-120 V
Safety standards
Certification: IEC-CB, CE and NRTL-TUV Rheinland of North America
The instrument fulfills the requirements of:
IEC 61010-1:2001, UL 61010-1:2004 R10.08, CAN/CSA C22.2
No. 61010-1-04+GI1 (R2009), IEC 61010-2-010:2003, CAN/CSA C22.2
No. 61010-2-010 (R2009)
Conformance to EU directives
The CE mark conforms to the following EU directives: 89/336/EEC
(as amended by 92/31/EEC) relating to Electromagnetic Compatibility
2006/95/EC (Low Voltage Directive)
Performance specifications used to verify conformance to the directives
above:
EN 55022 class B
IEC 61000-4-3/EN 61000-4-3 3 V/m
IEC 61000-4-2/EN 61000-4-2 4 kV CD, 8 kV AD
IEC 61000-4-4/EN 61000-4-4 1 kV
IEC 61010-1/EN 61010-1
Including Amendments 1 and 2
Technical design of ViewLux uHTS Microplate Imager
3
Specifications, continued
Environmental conditions
Operating conditions: +15 °C to +35 °C, Relative humidity 60% maximum at 28 °C, decreasing linearly to 40% at 35 °C
(indoor use)
Transportation conditions: 0 °C to +60 °C, Relative humidity 5 to 90%, IEC 68-2-56 as guidelines packed in transportation
package
Storage conditions: 0 °C to +60 °C, Relative humidity 5 to 90%, IEC 68-2-56 as guidelines packed in transportation package
Light source
Light source comprising up to 8 flash lamps, spectral range: 300 nm-600 nm.
Rotatable excitation filter wheel with 10 filter positions (Ø 60 mm). Filters can be changed by the user as required.
Detection unit
850 model back-illuminated CCD camera
Rotatable emission filter wheel, with 10 filter positions (Ø 60 mm). Filters can be changed by the user as required.
A TRF-shutter is used in TR-fluorescence to provide a gating window.
Plates
Up to 3456-well microtitration plates are compatible with the instrument.
The user can define non-standard plate configurations. The minimum outer dimensions are 85x127x0 mm and the maximum
outer dimensions are 89x129x25 mm.
Measurement time
Detection time per well is software selectable from 0.1 seconds upwards.
Physical dimensions
Weight of basic unit: 350 kg; Overall dimensions: 610x960x1800 mm (WxDxH)
Software
The instrument program is run on a Windows® XP based computer, minimum 256 MB memory, equipped with a CD-ROM,
super VGA display minimum resolution 1024x780 pixels, 65536 colors.
Input/output connections
Arcnet A and B; Cable from the camera to the PC
Stacker
The instrument can have none, one or two stackers. A stacker has place for 32 plates fitted into plate holders. The stacker is
designed to take plates that fulfill the following requirements: 127-129 x 85-89 x 0.5-25.0 mm (LxWxH)
Measurement modes
Prompt fluorometry
Fluorescence polarization
Time-resolved fluorometry
LANCE
Luminometry
Photometry
Custom mode
4
Specifications, continued
Barcode
Barcode length: max. 60 mm
Barcode height: min. 5 mm
Empty space at the ends of barcode label: min. 10 mm
Minimum bar width: min. 0.25 mm
Bar-space ratio: 1/3
Non-fluorescent label material
Code types:
• code39 (optional check digit)
• interleaved2/5 (optional check digit)
• codabar
• code128
Other barcodes may work, but should be tested individually.
Filters supplied
Filter type
Description
DUG11
Schott Color Glass Filter 340 nm ±30 nm
405/10
Narrow Band Interference Filter 405 nm ±5 nm
450/20
Narrow Band Interference Filter 450 nm ±10 nm
480/20
Narrow Band Interference Filter 480 nm ±10 nm
540/25
Narrow Band Interference Filter 540 nm ±12.5 nm
618/8
Narrow Band Interference Filter 618 nm ±4 nm
671/8
Narrow Band Interference Filter 671 nm ±4 nm
525/20
Narrow Band Interference Filter 525 nm ±10 nm
598/25
Narrow Band Interference Filter 598 nm ±12.5 nm
613/55
Narrow Band Interference Filter 613 nm ±27.5 nm
564/250
Absorbance Emission Filter 564 nm ±125 nm
Clear Infrared Damping Filter 400 nm – 750 nm
Black – A black opaque plate to act as a shutter
Polarizers for FP
Optional filters
Description
573/6
Narrow Band Interference Filter 573 nm ±3 nm
547/8
Narrow Band Interference Filter 547 nm ±4 nm
600/10
Narrow Band Interference Filter 600 nm ±5 nm
Filter systems for different measurement modes
Label system excitation emission
Prompt fluorescence:
UMB, AMC DUG11 450/20
FITC 480/20 540/25
Luminescence: Infrared damping filter
Absorbance: 405/10 (in excitation filter wheel)
480/20 (in excitation filter wheel)
564/250 (in emission filter wheel)
Black (in emission filter wheel)
5
Specifications, continued
Performance
Fluorescence in white 384-well plate with 50 μL sample volume
Fluorescein detection limit 5 fmol/well with FITC dichroic mirror, black plate
Umbelliferone detection limit 250 fmol/well
Rhodamine detection limit 25 fmol/well
Fluorescence in white 1536-well plate with 7.5 μL sample volume
Umbelliferone detection limit 100 fmol/well
TR-Fluorescence, DELFIA®, in white 384-well plate with 50 μL sample volume, with 30 s imaging time
Eu detection limit 50 amol/well with 2X binning
TR-Fluorescence, DELFIA, in white 1536-well plate with 7.5 μL sample volume, with 30 s imaging time
Eu detection limit 500 amol/well
Luminescence in white 384-well plate with 50 μL sample volume and with 1 min imaging time
Firefly Luciferase detection limit 5 fg/well with 4X binning
Luminescence in white 1536-well plate with 7.5 μL sample volume and with 1 min imaging time
Firefly Luciferase detection limit 25 fg/well with 2X binning
Absorbance in black, clear bottom 384-well plate with 50 μL sample volume
Photometric detection in the range of 0-1 absorbance units, 405 nm filter and Tartrazine samples.
Imaging wavelength 400 nm-800 nm
Spatial resolution 3 lp/mm
Dynamic range of the camera 16 bits/pixel
Uniformity of the plate <5% CV
Signal reproducibility CV <5%/1min/plate within 100 plates
Fluorescence polarization in black Costar® (Corning Inc.) 384-well plate with 40 μL sample volume. Samples 1 nM FITC and
1 nM BODIPY TMR
Imaging time: 30 s for FITC and 45 s for BODIPY
Intraplate standard deviation FITC 5 mP , BODIPY TMR 5 mP
Fluorescence polarization in black Greiner 1536-well plate with 5 μL sample volume
Samples 1 nM FITC and 1 nM BODIPY TMR
Imaging time: 60 s for FITC and 90 s for BODIPY
Intraplate standard deviation FITC 6 mP, BODIPY TMR 8 mP
Absorbance in black, clear bottom 1536-well plate with 7.5 μL sample volume
PerkinElmer, Inc.
940 Winter Street
Waltham, MA 02451 USA
P: (800) 762-4000 or
(+1) 203-925-4602
www.perkinelmer.com
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Copyright ©2011, PerkinElmer, Inc. All rights reserved. PerkinElmer® is a registered trademark of PerkinElmer, Inc. All other trademarks are the property of their respective owners.
009980_01
Nov 2011
Printed in USA