Designer Benzodiazepines

Designer Benzodiazepines
Solutions for Forensic Drug Detection
Summary
Neogen’s Benzodiazepine Group ELISA kit provides ultra
sensitive detection of over 20 drug analytes in a variety of
matrices. Designer benzodiazepines are increasingly widespread
throughout Europe as recreational drugs and non-prescription
medication. Our test kit is successful in the detection of both
traditional and synthetic benzodiazepines and is intended for
forensic use only. It is recommended that all questioned
samples be confirmed by a quantitative method such as gas
chromatography/mass spectrometry (GC/MS).
Product Features
• Ultimate detection of major drug analyte oxazepam
• Ultra-sensitive detection of more than 20 benzodiazepines
including phenazepam, etizolam, delorazepam, diclazepam,
pyrazolam, flubromazepam, alprazolam, lorazepam, clonazepam,
diazepam and temazepam
• Validated for human urine, whole blood and oral fluid
• Detectability in other forensic matrices
• Single and bulk packaging available
• Compatibility with automated and semi-automated instruments
Summary of Protocol
- Add 10 µL samples or calibrators
- Add 100 µL ready-to-use enzyme conjugate solution
- 45 minute incubation at room temperature
- Wash plate 3 times
- Add 100 µL TMB Substrate
- 30 minute incubation at room temperature
- Add 100 µL Stop Solution
- Measure OD at 450 nm
Sample Dilutions
The recommended minimum
sample dilution is 1:10 for urine
and 1:5 for whole blood
and oral fluid.
Order Details
96 well package
480 well package
Product No. 130119
Product No. 130115
For more information or to place an order please contact:
Neogen Europe Ltd. The Dairy School • Auchincruive, Ayr, KA6 5HU • Scotland • UK
Tel: +44(0)1292 526 092 • Fax: +44(0)1292 525 602 • www.neogeneurope.com • tox@neogeneurope.com
©Neogen Corporation, 2014. Neogen is a registered trademark of Neogen Corporation, Lansing, Mich, USA.
Designer Benzodiazepines
Solutions for Forensic Drug Detection
Sensitivity and Specificity
- Indicates Designer Benzodiazepines
Cross-Reactivity Profile
Drug
I-50 (ng/ml)
Percent Cross-Reactivity
Oxazepam
5.81
100
Diazepam
1.34
434
Estazolam
1.6
365
Nordiazepam
1.61
361
Alprazolam
1.68
346
Tetrazepam
2.2
264
Flurazepam
2.22
262
Lormetazepam
2.52
231
Prazepam
2.94
198
Temazepam
3.02
192
Phenazepam
3.05
190
Halazepam
3.36
173
Triazolam
3.39
171
7-amino-Flunitrazepam
3.94
147
Diclazepam
4.10
142
β-Glucuronidase Treatment of Samples
If detection of total benzodiazepines including the glucuronide
metabolites is desired, an initial sample treatment with a
hydrolysis enzyme will be necessary.
Neogen's β-Glucuronidase treatment will hydrolyze the
glucuronic acid from lorazepam glucuronide to yield
unconjugated lorazepam for better detection of total
lorazepam in Neogen’s Benzodiazepine Group ELISA Kit (see
figure below)*.
Nitrazepam
4.11
141
Flubromazepam
4.83
120
N-desmethyl Flunitrazepam
4.89
119
Delorazepam
5.04
115
Flunitrazepam
5.29
110
β-Glucuronidase Treatment Order Details
Pyrazolam
5.49
106
Etizolam
6.70
87
Bromazepam
6.8
85
Clonazepam
7.31
79
Neogen's β-Glucuronidase treatment is available as a
2-bottle reagent pack in our Accessory Reagents product
line. For additional information, please contact a forensic
respresentative.
Nimetazepam
7.90
74
Lorazepam
8.27
70
Midazolam
8.93
65
Clobazam
9.93
59
7-amino-Clonazepam
14.78
39
Alpha-hydroxy Alprazolam
23.65
25
Chlordiazepoxide
31.03
19
β-Glucuronidase Reagent Pack
*The ββ-Glucuronidase sample treatment for lorazepam glucuronide should be able to be utilised for
other glucuronide metabolites of other drugs. Neogen's β-Glucuronidase sample treatment has only
been tested on lorazepam glucuronide and Neogen’s Benzodiazepine Group ELISA Kit. It has NOT been
tested on any other glucuronide metabolites and/or forensic detection kits.
For more information or to place an order please contact:
Neogen Europe Ltd. The Dairy School • Auchincruive, Ayr, KA6 5HU • Scotland • UK
Tel: +44(0)1292 526 092 • Fax: +44(0)1292 525 602 • www.neogeneurope.com • tox@neogeneurope.com
©Neogen Corporation, 2014. Neogen is a registered trademark of Neogen Corporation, Lansing, Mich, USA
Product No. 301605