Mahvash Khodabandeh

Transcription

Mahvash Khodabandeh
Mahvash Khodabandeh Shahraky
National Institute of Genetic Engineering and Biotechnology (NIGEB),
Shahrak-e- Pajoohesh, 15th Km, Tehran -Karaj Highway, Tehran, Iran.
P.O. Box: 14965 -161, Tehran, Iran.
Phone: +98- 21-44787355
Fax: +98-21-44787399
E-mail: saba@nigeb.ac.ir
Website: www.nigeb.ac.ir
EMPLOYMENT (Present & previous positions)

Faculty Member, Industrial and Environmental Biotechnology Institute, 1995-now.
Education

Ph.D. (1990 -1995), Biochemistry, Tehran University

M.Sc. (1987 -1989), Biochemistry, Tehran University

B.Sc. (1983 – 1986), Zoology, Shahid Beheshti University, Tehran
ACTIVE RESEARCH PROJECTS

Downstream processing (Purification of recombinant proteins)

Protein folding

Scale up of protein purification



Development of Transdermal drug delivery systems
Environmental Biotechnology
Starter culture development
PRESENT AND PREVIOUS POSITIONS:

In charge of biological and chemical materials, NIGEB (December 1997- 2006)

Member of the Council of Supervising and Evaluation of the Institute Faculty Members (1998-2002)

Member of the executive committee of Human Growth Hormone Production Project (1999-2007)

Top Faculty Member of NIGEB (1999)

In charge of research support, NIGEB (July 2000- 2007)

Member of the committee of “Determining the Standards of Recombinant Medications”, Ministry of Health
(2001-2003)

Director of Education Office, National Institute of Genetic Engineering and Biotechnology (NIGEB), (September
2001- January 2004)

Member of the Committee of “Compiling the National Strategy of Biotechnology” (2001-2003)

Member of Strategic Committee of Iran Membership in Stockholm Convention (2003-2007)
1

In charge of laboratories, NIGEB, (December 2004- 2006)

Head of the Fermentation Industries Department and Member of NIGEB Council (2006-2008)

Supervisor of laboratory materials, NIGEB

Head of the Bio-process Department

Member of the “Publishing Committee of the Institute”

Member of directing board of the Zist Farayand Pars Knowledge- Based Company

In charge of Intellectual Property Office
COURSE TAUGHT:
1. Down stream processing
2. Principles of Biohysics and Biochemistry
3. Proteomics
4. Structures and Functions of Proteins
5. Industrial Biotechnology Principles
6. Biotechnology
7. Biochemistry
8. Protein Engineering
9. Advanced Genetic Engineering 2
10. Advanced Animal Genetics
NATIONAL RESEARCH PROJECTS:

Purification of recombinant human growth hormone

Production of recombinant human -Interferon in E.coli

Increasing of recombinant INF- purification efficiency

Investigation on skin absorption of recombinant human growth hormone

Investigation of the effective oral component of leech on rat`s nervous system.

Study of the effect of human HSP90 on the conformational structure and immunogenicity of recombinant core
and HbsAg protein of HCV and HBV viruses.

release.
Preparation of transferosome growth hormone loaded and optimization of loading efficiency and study of its

Biological treatment of phenolic wastewater of petrochemical industries.

Production of human recombinant GM-CSF in pilot plant scale. (Collaborator)

Cloning and expression analysis of human factor VIII cDNA in mammalian expression system. (Collaborator)

Molecular approach to achieve efficient expression of recombinant human factor VIII in mammalian cells.
(Collaborator)
2
AWARDS / MEMBERSHIPS


Iranian Society of Biochemistry.
Iranian Society of Biotechnology.

Iranian Society of Nanotechnology.
WORKSHOPS ORGANIZED

Gene expression and protein purification


Large scale protein purification
GMP

Validation and documentation
PUBLICATIONS (English)
2010-2015
1.
Leila Badiefar, Bagher Yakhchali, Susana Rodriguez-Couto, Antonio Veloso, José Ma García-Arenzana,
Yoshinobu Matsumura and Mahvash Khodabandeh: Biodegradation of bisphenol A by the newly
isolated Enterobacter gergoviae strain BYK-7 enhanced using genetic manipulation. Royal Society of
Chemistry 2015, 5. 29563-29572.
2.
Mojtaba Samoudi, Fatemeh Tabandeh, Zarrin Minuchehr, Reza Ahangari Cohan, Davoud Nouri
Inanlou, Mahvash Khodabandeh, Mohammad Sabery Anvar: Rational design of hyper-glycosylated
interferon beta analogs: A computational strategy for glycoengineering. Journal of Molecular Graphics and
Modelling, 2015, Vol.56, Page: 31-42
3.
Farzaneh Ashnagar, Mahvash Khodabandeh, Ayyoob Arpanaei, Zohreh Azita Sadigh, Fatemeh Rahimi,
Parvin Shariati: Optimizing Primary Recovery and Refolding of Human Interferon- from Escherichia
coli Inclusion Bodies. Iranian Journal of Biotechnology 2015 (Issue 4)
4.
Mohieddin Jafari, Hassan Rezadoost, Mehrdad Karimi, Mehdi Mirzaie, Mostafa Rezaie-Tavirani,
Mahvash Khodabandeh, Gholamreza Kordafshari, Kambiz Gilany, Nafiseh Abbasian, Payman
Nickchi, Alireza Ghassempour: Proteomics and Traditional Medicine: New Aspect in Explanation of
Temperaments. Forschende Komplementärmedizin / Research in Complementary Medicine 08/2014; 21(4).
DOI:10.1159/000366118
5.
Mohammad
Azizi,
Bagher
Yakhchali,
Abdolreza
Ghamarian,
Somayeh
Enayati,
Mahvash
Khodabandeh, and Vahid Khalaj: Cloning and Expression of Gumboro VP2 Antigen in Aspergillus
niger. Avicenna Journal of Medical Biotechnology Vol 5, No. 1, January-March 2013
6.
Sharifnia Z, Mosaffa N, Khodabandeh M, Yaraee R, Kazemi B, Bandehpour M: Human βhsp 90 as
agjuvant in HCV recombinant vaccine. J of Paramedical Sciences (JPS) 2013, 4(3):99‐103.
7.
Pooreydy B, Jafari M, Tajik F, Karimi M, Rezai‐Tavirani M, Ghassempour A, Rezadoost H,
Khodabandeh M: Organel isolation for protemeomics for proteomics: mitochondria from peripheral
blood mononuclear cells. J of Paramedical Sciences 2013, 4:78‐86.
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8.
Karkhaneh AA, Yakhchali B, Rastgar‐Jazii F, Hemmat I, Shariati P, Khodabandeh M, Zomorodipoor A:
Priplasmic expression of Bacillus thermocatenulatus lipase in E. coli in presence of different signal
sequences. Iranian Journal of Biotechnology 2012, 10(4):255‐262.
9.
Shojaadini M, Moharramipour S, Khodabandeh M, Talebi AA: Mechanisms contributing epizpptics of
Bacillus thuringiensis in plodia interpunctella populations. Integrated Protection of Stored Products
IOBC/wprs Bulletin 2011, 69:169‐173.
10. Sharifzadeh‐Baei M, Najafpour GD, Yonesi H, Tabandeh F, Issazadeh H, Khodabandeh M: Growth
kinetic parameters and biosynthesis of polyhydroxy butyrate. Chemical Industry & Chemical Engineering
Quarterly 2011, 17(1):1‐8.
11. Aghaeepoor M, Mozafari S, Khodabandeh M, Tabandeh F, Bambai B: High level of extracellular
fermentation and alternative purification of E. coli Asparaginase II. Bihaream Biologist 2011, 5(2):96‐101.
12. Shakory R, Khodabandeh M, Toliyat T, Azimzadeh S, Shadigh ZA, Badifar L: Enhancing effect of
cyclodextrins on in vitro skin permeation of hGH. Current Trends in Biotechnology 2010, 4(3):784‐790.
13. Bandehpour M, Khodabandeh M, Mosaffa M, kazemi B: An efficient procedure for purification of
recombinant human β heat shock protein 90. DARU 2010, 18(1):64‐68.
2005-2009
14. Shojaadini M, Moharramipour S, Khodabandeh M, Talebi A: Development of a cost effective medium
for production of Bacillus thuringienses. Journal of Plant Protection. 2010, 50(1):9‐14.
15. Mehrabadi JF, Behzadian‐Nejad Q, Najar Sh, Khodabandeh M: Construction of pc DNA/fimH cassette
as a DNA vaccine candidate. Res J of Microb 2009, 4(2):75‐81.
16. Khodabandeh M, Badiefar L, Ahmadian G, Asgharani E, Ghandili S, Salek‐Esfahani M: Optimization of
conditions for Expression and Activation of a splice variant of prochymosin lacking exon 6 in Escherichia
coli. The International Journal of Dairy Technology. 2009, 62(2):265‐271.
17. Amirkhani H, Asgarani E, Khodabandeh M: Importance of catalase in adaptive response and resistance
to to hydrogen peroxide in Haloferax IRU1. The Int J of Microbiology 2009, 7(1):1‐11.
18. Tabandeh F, Khodabandeh M, Yakhchali B, Ghomi HH, Shariati P: Response Surface Methodology for
Optimizing the Induction Conditions of Recombinant Interferon beta during high cell density culture.
Chemical Engineering Science 2008, 63:2477‐2483.
19. Ghane M, Yakhchali B, Khodabandeh M: Over expression of biologically active Interferon Beta using
synthetic gene in E. coli. J of Sci IR of IRAN 2008, 19(3):203‐209.
20. Bandehpour M, Khodabandeh M, Kazemi B: Cloning and Expression of Hepatitis B Surface Antigen.
Hepatitis Monthly 2008, 8(1):17‐21.
21. Ghane M, Yakhchali B, Khodabandeh M, Malekzadeh F: Design, Construction and
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Expression of a Synthetic β‐Interferon (IFN‐ β) Gene in E.coli. Pakistan Journal of Biological Sciences 2006,
9(15).
22. Amiri‐Yekta A, Zomorodipour A, Khodabandeh M, Chopari MD: Bacterial expression and purification
of C1C2 domain of human factor VIII. Iranian Journal of Biotechnology 2006, 4(2).
23. Mizani M, Aminlari M, Khodabandeh M: An effective method for producing a nutritive protein extract
powder from shrimp‐head waste. Food Science and Technology International 2005, 11(1).
24. Khodabandeh M, Salek M, Zomorodipour A, Deezagi A, Madah T: Purification of biologically active
B‐domain deleted recombinant human Factor VIII in mammalian cells. Clinical Biochemistry 2005, 38.
25. Hashemi H, Jourabchi E, Khodabandeh M: Transient expression of hGH in potato,
tobacco and lettuce leaves by agroin filtration. Iranian Journal of Biotechnology 2005, 3(2).
26. Atri MS, Sabouri AA, Rezaei M, Sanati MH, Moosavi AA, Sadeghi M, Mansuri H, Khodabandeh M:
Binding properties and conformational change of human growth hormone upon interaction with Fe3 +.
Thermochimica Acta 2005, 438.
27. Amirizadeh N, Zomorodipour A, Deezagi A, Khodabandeh M, Rastegar G, Salek M: Expression of
biologically active recombinant B‐domain‐ deleted human factor VIII in mammalian cells. Journal of
Sciences, IR of Iran 2005, 16(2).
Before 2005
28. Zomorodipour A, Yakhchali B, Khodabandeh M, Deezagi A, Valian S, M.Hosseini S: The over‐
expression of biologically active hGH in a T5‐ based system in E.coli, studying temperature effect.
Journal of Sciences, IRof Iran 2004, 15(1).
29. Tabandeh F, Yakhchali B, A.Shojaosadati S, Khodabandeh M, H.Sanati M: Growth kinetics and hGH
production of a heat inducible recombinant E.coli during batch fermentation. Iranian Journal of Science
and Technology Transaction A 2004, 28(A1).
30. Tabandeh F, Shojaosadati SA, Zomorodipour A, Khodabandeh M, H.Sanati M, Yakhchali B:
Heat‐Induced production of hGH by high cell density cultivation of recombinant Escherichia coli.
Biotechnology Letters 2004, 26.
31. Khavarpour M, Tabandeh F, Jahanshahi M, Khodabandeh M, Danesh H: ATPS as an
efficient method for sepration of bionanoparticles Investigation. 2004, 23(3):351‐357.
Ghasemi F, Zomorodipour A, Shojai S, Ataei F, Khodabandeh M, Sanati MH: Using LArabinose for
production of human GH in E.coli, studying the processing of gIII: HGH precursor. Iranian Journal of
Biotechnology 2004, 2(4).
32. Khodabandeh M, Yakhchali B, Rahimi M, Vaseli N, Zomorodipour A, Deezagi A: Purification of large
quantities biologically active rhGH. Iranian Journal of Biotechnology 2002, 1(4).
33. Khodabandeh M: The effect of Mechloroethamin on reconstituted chromatin. Medical Journal of the
Islamic Republic of IRAN 1997, 11(1).
5
34. Rabbani A, Sharoghi B, Khodabandeh M: Interaction of Doxorubicin with DNA‐HMG1 complex.
Journal of Sciences Islamic Republic of Iran 1995, 6(3).
35. A.Rabbani, Khodabandeh M: Studies on the Binding of the Alkylating Agent Sulfur Mustard to Calf
Thymus Chromatin. Medical Journal of the Islamic Republic of IRAN 1993, 7(1).
PUBLICATIONS (Farsi)
BOOK/BOOK CHAPTER
1. Khodabandeh M. (2015) “Structure and Function of Proteins of Life Molecules”. ISSBN: 978‐964‐8516-22-7
2. Fatemeh Tabandeh, Parvin Shariati, and Mahvash Khodabandeh. (2012) Application of TwoDimentioanl Gel
Electrophoresis to microbial systems, Gel Electrophoresis Principals and Basics. ISSBN: 978‐953‐51‐0458‐2
PATENTS/REGISTRATION
1. Khodabandeh M. One step purification method of recombinant Human Growth Hormone based
on pH precipitation, Iranian Patent No: 30309
2. Tabandeh F. Khodabandeh M. The stabilizing medium of lactic bacteria in freeze drying method,
Iranian Patent No: 80657
3. Production of solvable starter culture to be used in preparing yogurt, Iranian Patent No: 70020
4.
Tabandeh
F.
Khodabandeh
M.
Yakhchali
B.
Habibghomi
H.
Recombinant
β-interferon
production based on recombinant E-coli, Iranian Patent No: 38704594
PROCEEDINGS/ABSTRACTS (English)
1. Khodabandeh M:َ A simple method for Purification of Law Levels of Bbeluga (Huso Huso) Vitellogenin .
In: 2012 International Conference on Applied Life Sciences; 2012.
2. Shakory R, Khodabandeh M, Toliyat T: Comparative study of skin penetration of hGH. In: 14th IBS 2010;
Rimini; 2010.
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3. Farahmand B, Khodabandeh M, Mahboodi F, Fotouhi F, Saleh M, Bakhordari F: Cloning and expression
of Influenza virus A (H1N1). In: 14th IBS: 2010; Rimini; 2010.
4. Badiefar L, Khodabandeh M, Ahmadian G: Evaluation of the recombinant E.coli strains producing
prochymosin. In: 14th IBS: 2010; Rimini; 2010.
5. Fallah J, Behzadian‐Nejad G, Pyrayeh S, Khodabandeh M, Soleimanjahi H, Zohair MH: Construction of
pcDNA/FIMH Cassette Infection and Evaluation of FIMH Expression in cos7 cell line. In: The 1st
International congress on Health Genomics & Biotechnology: 2007; Summit Meeting Conference Hall, Tehran, Iran;
2007.
6. M.Ghane, B.Yakhchali, Khodabandeh M, F.Malekzadeh: Construction of a synthetic gene for production
of IFN‐ β in E.coli. In: 8th Biochemistry Congress: 2005; Tarbiat Modares University ,Tehran, Iran; 2005.
7. Khodabandeh M, Salek M, Deezagi A, Zomorodipour A: Purification of biologically active B‐ domain
deleted r‐hFVIII in mammalian cells. In: 8th Biochemistry Congress: 2005; Tarbiat Modares University, Tehran,
Iran; 2005.
8. Khodabandeh M, Rabbani A, Jafari M: The effect of sulfur mustard on histone proteins in chromatin. In:
8th Biochemistry Congress: 2005; Tarbiat Modares University ,Tehran, Iran; 2005.
9. Ghasemi F, Zomorodipour A, Shojai S, Ataei F, Khodabandeh M, Sanati MH: Periplasmic expression of
hGH in E. coli using L‐Arabinose induction. In: 8th Biochemistry Congress: 2005; Tarbiat Modares University
,Tehran, Iran; 2005.
10. Ghasemi F, Zomorodipour A, Shojai S, Ataei F, Khodabandeh M, H.Sanati M: Bacterial production of
human Growth hormone
using Arabinose‐Induction. In: 1st International Conference on Environmental
Industrial and Applied Microbiology: 2005; Spain; 2005.
11. Ghane M, Yakhchali B, Khodabandeh M, Malekzadeh F, Danesh HA: Construction of IFN‐ β gene by step
wise assembly PCR method according to E.coli codon usage. In: 13th Conference of Biology: 2005; Tehran,
Iran; 2005.
9. Mozaffari S, Aghaeepoor M, Khodabandeh M, Lotfi AS, Bambai B: Designing of expression systems and
optimization for high‐yield extra cellular production of recombinant L‐Asparaginase II from Escherichia Coli in
shaking‐flask and bioreactor. In: 9th Congress of Biochemistry of Iran and 2nd International Congress of Biochemistry
and Molecular Biology: 1386; Medical Science University of Shiraz; 1386.
10. Khodabandeh M, Salek M, Sadigh ZA, Ghomi HH: Investigating the solubilization of Inclusion bodies (Ib)
containing recombinant Interferon‐ β‐1b. In: 9th Congress of Biochemistry of Iran and 2nd International Congress of
Biochemistry and Molecular Biology: 1386; Medical Science University of Shiraz; 1386.
11. Bambai B, Torkynejad F, Ebrahimpour GH, Khodabandeh M: Molecular cloning and expression of the
Arthrobacter Viscosus Penicillin G Acylase gene in pET21 vector. In: 9th Congress of Biochemistry of Iran and 2nd
International Congress of Biochemistry and Molecular Biology: 1386; Medical Science University of Shiraz; 1386.
12. Badiefar L, Khodabandeh M, Ahmadian GR, Fatemi SA, Asgarani E, Salek M: Comparison of expression of
two different forms of prochymosin gene in two E. coli BL21 recombinant strains. In: 9th Congress of
7
Biochemistry of Iran and 2nd International Congress of Biochemistry and Molecular Biology: 1386; Medical Science
University of Shiraz; 1386.
13. Amirkhani H, Khodabandeh M, Asgarani E, Fatemi SA: Determination of the optimum growth conditions
of Iranian Haloferax isolated from Uremia Lake by the Taguchi method.
In: 9th Congress of Biochemistry of Iran and 2nd International Congress of Biochemistry and Molecular Biology: 1386;
Medical Science University of Shiraz; 1386.
14. Abbasi N, Zomorodipour A, Khodabandeh M, Mirdamadi S: Study of periplasmic expression of human
growth hormone using synthetic signal peptides designed based on TAT and SEC secretion pathway. In: 9th
Congress of Biochemistry of Iran and 2nd International Congress of Biochemistry and Molecular Biology: 1386; Medical
Science University of Shiraz; 1386.
15. Tabandeh F, Khodabandeh M, Jahanshahi M, Khavarpour M, Danesh HA: Investigation of the
partitioning behavior of plasmid DNA as a nanobioparticle in PEG300/salt aqueous two phase system. In: 11th
Congress of Chemistry Engineering of Iran: 1385; Tarbiat Modares University, Tehran; 1385.
16. Yekta AA, Zomorodipour A, Khodabandeh M, Daliri M, Ataei F: Use of bacterially derived hFVIII‐ light
chain sub ‐fragment to develop specific antibody against the plasma derived hFVIII. In: 4th conference of
Biotechnology: 1384; Kerman,Iran; 1384.
17. Kiani J, Zomorodipour A, Khodabandeh M, Foroozandeh M, Fatemi SA: Secretory production of rhGH in
E.coli in presence of lactose as inducer using pel B signalsequence. In: 4thconference of Biotechnology: 1384;
Kerman, Iran; 1384.
18. Ghane M, Yakhchali B, Khodabandeh M, Malekzadeh F, Danesh HA: Construction and cloning of a
synthetic gene for expression of IFN‐ β in E.coli under the control of Lac and TAC promoters. In: 4th
conference of Biotechnology: 1384; Kerman,Iran; 1384.
19. Ghane M, Yakhchali B, Khodabandeh M, Malekzadeh F: The use of synthetic gene for cloning and
expression of IFN‐ β in E.coli. In: 1st International conference of Environmental Industrial and Applied
Microbiology: 2005; Spain; 2005.
20. Khodabandeh M, Deezagi A, Sanati MH: Effect of purification condition of r‐hGH on receptor binding
profile. In: 7th IUBMB: 2002; Norvay; 2002.
21. Kiani J, Zomorodipour A, Khodabandeh M, Foroozandeh M, Sanati MH: Periplasmic production of
r‐hGH E. coli using T7 promoter and omp A signal sequence. In: 3th conference of Biotechnology: 1382;
Ferdosi University of Mashhad, Iran; 1382.
8
PROCEEDINGS/ABSTRACTS (Farsi)
A
A
A
-
B
HSP 95
core
HSP95
A
COS7
pcDNA/fimH
9
E. coli
Alcanivorax borkumensis
Arthrobacter viscosus
Rubredoxinreductase
Rubredoxin
E. coli BL21
E. coli
T7
T7
GM-CSF
refolding
11
hGH-107
E. coli
–
Affinity
pH
Supervised Research Theses
1. Production of Poly Hydroxy Alkanate and its Application in Polymeric Nano Composites
(Maziar Sharifzadeh)
2. Beluga (Huso huso) Vitellogenin: Purification, Parial Characterization and Possibility of Antibody
Production (Mahdi Paktinat)
3. Biodegradation of Bis Phenol A in Salty Condition by Indigenous Bacteria (Sasan Jahanshahi)
4.
Effect
of
Transfersome
Containing
Growth
Hormone
on
Proliferation
of
Dermal
Fibroblast
(Minoo Azimi)
5. Cloning and Exprssion of Human Coagulation Factor VIII Heavy Chain and Producing PolyClonal Antibody Against it (Mahin Salmannejad)
6. Effect of α-amylase Signal Peptide from Bacillus Licheniformis on Secretion of Recombinant
Human Growth Hormone by E. Coli (Farzaneh Moshiri)
7. Application of Reverse Micellar System for Purification of Denatured r-hGH (Leila Pishkar)
8. Study on Effect of Human HSP 90 in Conformation and Immunity of HCV Core Protein and HBV
Hbs Ag. (Mojgan Bandehpour)
9. Isolation and Identification of Local Probiotic Lactobacillus Strain: Its Encapsulation to Improve
Bacterial Viability (Nafiseh Sadat Frotan)
11
10. Periplasmic Expression of Human Growth Hormone, Using Synthetic Signal Peptide, Designed
Based on TAT and Sec Pathways (NAsrin Abbasi)
11.
Preparation
of
Transfersomes
of
Growth
Hormone
Loaded
and
Optimization
of
Loading
Efficiency and Study of its Release (Maryam Kateh Shamshiry)
12. Skin Absorption of Recombinant Human Growth Hormone (Roghayeh Shakoory)
13. Protein Extraction from Artemia Urmiana and Studying the Anti-aging Effects on these Agents
of Human Fibroblast Cell Culture Medium (Neda Vaselli Hagh)
14. The Effect of the Purification pH on the Pharmacological Characteristic of Recombinant Human
Growth Hormone (Ensieh Ganjpour Sales)
15. Evaluation of eNOS and iNS Genes Expression in Broiler’s Pulmonary Hypertension due to
Cold Stress (Hossain Hassanpour)
16. The Effect of the Alpha-Interferone on Human Fibroblast Cells (Saeid Ansari Majd)
17. Periplasmic Expression of Recombinant Human Growth under the Control of ara BAD Promoter
in E. Coli
18.
Physio
Patology
of
Strain
Bacillus
Thuringiensis
EF495116
in
Indian
Meal
Moth
Plodia
Interpunctella Hubner and Diamond Back Moth Pluntella Xylostella (L.) (Mohammad Shojaaddini)
19. Refolding and Purification Yield of Recombinant Human Beta Interferon (Farzaneh Ashnagar)
20. Recombinant Expression of Rubredoxin Reductase from Alcanirorax Borkumensis in E. Coli
System (Fatemeh Amoozadeh)
21. Production and Insitu Separation of Lactic Acid by Lacto Bacillus Casei in a Batch Fermentation
of Whey (Mohammad Sadegh Abdolalizadeh)
22. Cloning and Expression of Beta Interferon in E.coli (Maryam Ghane)
23. Evaluation of Surface Modification of Beta Interferon by Activated PEG. (Ahmad Abolhasani)
24. Optimization of Human Factor VIII Purification from Serum and Generalization to Recombinant
VIII Factor (Mojgan Salek Esfahani)
25.Study of Amylase and Protease Co-Production by Bacillus Licheniformis (Elham Asghari Adib)
26. Heat-induced Production of Human Growth Hormone by High Cell Density Cultivation of
Recombinant E. coli (Fatemeh Tabandeh)
27. Cloning and Expression of Active Single Chain Penicillin G acylase (Sareh Zeydabadinejad)
28. Cloning and Expression of Glucose Oxidase Gene in E. Coli (Ameneh Mohaghegh)
29. Cloning and Expression of Alkane Hydroxylase-I from Marine Alcanivorax borknmensis SK2 in
E.coli System (Simin Zadegan Eydani)
30. Study on Catalase Features from Haloferax Isolated from Urmia Lake and its Role in the
Resistance against Oxidative Stress (Homeira Amirkhani)
12
31. Effect of L-Cystein and Method of Cooking on Reologic and Electro Phoretic Patterns of Sausage
(Sayed Ebrahim Hosseini)
32. Isolation and Purification of Artemia Extract and Effects of this Extract on Proliferation or
Apoptosis of Human Leukocytecells HL-60 (Azadeh Chashnidel)
33. Expression of Influence Virus Membrane Glycoprotein (Hemagglutinin) in Recombinant E. coli
(Behrokh Farahmand)
34. Studying of the DNA Breakdown and Enucleation during Differentiation on Process of the
Human Erythroleukemic K562 Cell Line and Relation of this Events with Telomerase Activity
(Mahkameh Abedi)
13

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