SIF Powder FaSSIF-V2 How To Use

SIF Powder FaSSIF-V2 How To Use
Document Name:
SIF Powder FaSSIF-V2 How To Use
Document Version:
1.0
This User Guide explains how to make 1 Litre of FaSSIF-V2. For any other volume use the Calculator
on our website http://biorelevant.com/fassif-v2/how-to-make.
How to make 1 litre of FaSSIF-V2
Step 1
Preparation of maleate buffer for FaSSIF-V2 (1 Litre):
Dissolve:
1.39 g NaOH (pellets)
2.23 g of maleic acid
4.01 g of NaCl
in 0.9 L of purified water
Adjust the pH to 6.5 with either 1 N NaOH or
1 N HCl and make up to volume (1 L) with purified
water
Step 2
To make 1 litre of FaSSIF-V2:
• Add 1.79 g of SIF Powder FaSSIF-V2 to about
500 mL of buffer at room temperature
• Stir until SIF Powder has dissolved
• Make up to volume (1 L) with the buffer
Let FaSSIF stand for 1 hour. FaSSIF-V2 is ready for
use.
Storage and Handling
• Refrigerate SIF Powder FaSSIF-V2 bottle at 2-8°C
• Before opening, equilibrate SIF Powder FaSSIF-V2
bottle to room temperature
• Use the FaSSIF-V2 you have made within 48 hours
at room temperature and 24 hours at 37°C
Drug Analysis
*Use HPLC to measure drug content*
Any questions? Contact us: info@biorelevant.com
Instant Powders for the preparation of biorelevant media are covered by PCT/EP2006/010809.
b i o r e l e v a n t . c o m , U n i t 2 0 , C a p i t a l B u s i n e s s C e n t r e , 2 2 C a r l t o n R d . , C R 2 0 B S , U n i t e d K i n g d o m Tel: +44
(0)20 7609 3499 E-Mail: info@biorelevant.com Webpage: http://www.biorelevant.com
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