A P S

24TH ANNUAL MEETING OF THE
DUTCH SOCIETY FOR
CALCIUM AND BONE METABOLISM
PROGRAM AND ABSTRACTS
Zeist, the Netherlands
November 12-13, 2014
SPONSORS
AMGEN
LILLY
ALEXION
GREINER BIO-ONE
LONZA
LA BIOSYSTEMS
24TH ANNUAL MEETING OF THE DUTCH SOCIETY FOR
CALCIUM AND BONE METABOLISM
November 12-13, 2014
Woudschoten Conference Centre
Woudenbergseweg 54, Zeist
The Netherlands
 + 31 343 492492 - Fax +31 343 492444 – www.woudschoten.nl
Organisation
DUTCH SOCIETY FOR BONE AND CALCIUM METABOLISM
Carola Zillikens, President
Vincent Everts, Secretary
Bram van der Eerden, Treasurer
Secretariat: Dept Oral Cell Biology
ACTA – VU University Amsterdam
Gustav Mahlerlaan 3004 – 1081 LA Amsterdam - The Netherlands
 +31 20 5980882 – www.nvcb.nl
e-mail: v.everts@acta.nl
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24 MEETING OF THE DUTCH SOCIETY FOR CALCIUM AND BONE METABOLISM
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SPONSORS
The Dutch Society for Calcium and Bone Metabolism is grateful for the generous
support of the following companies:
CORPORATE SPONSORS
SILVER SPONSORS
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24 MEETING OF THE DUTCH SOCIETY FOR CALCIUM AND BONE METABOLISM
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PROGRAM
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Wednesday, November 12, 2014
12.00-13.00
Registration + lunch
SESSION I
Chair: Jing Guo
13.00-13.10
Mechanical loading reduces inflammation-triggered
human osteocyte-to-osteoclast communication
Janak L. Pathak1,2, N. Bravenboer3, F.P. Luyten2, P.
Verschueren2, W.F. Lems4, J.Klein-Nulend1, A.D.
Bakker1
1
ACTA-VU Oral Cell Biology, 2 KU Leuven, Skeletal Biology
and Engineering Research Center, 3 VUmc Clinical
Chemistry, 4 VUmc Rheumatology
p. 13
13.10-13.20
Bone material strength as measured by microindentation
in vivo is decreased independently of bone mineral
density in patients with fractures
Frank Malgo, N.A.T. Hamdy , S.E. Papapoulos, N.M.
Appelman-Dijkstra
Center for Bone Quality, Leiden University Medical Center,
Leiden
p. 14
13.20-13.30
“Atypical” atypical femur fractures and use of
bisphosphonates
Denise M. van de Laarschot1, M.P. Somford2, A. Jager3, P.K.
Bos4, M.C. Zillikens1
1
Erasmus MC Rotterdam, Internal Medicine, 2Medisch
Spectrum Twente Enschede, Orthopedic Surgery, 3Erasmus
MC Cancer Institute, Medical Oncology, 4Erasmus MC,
Orthopedics
p. 14
13.30-14.20
Key-note lecture:
How to measure bone quality in daily practice
Joop van den Bergh
VieCurie Medical Center, Venlo, the Netherlands and Hasselt
University, Belgium
14.20-14.50
Coffee/Tea break
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SESSION II
Chair: Janak Pathak
14.50-15.00
Short-term effect of estrogen on human bone marrow fat
Eelkje J. Limonard*1, A.G. Veldhuis-Vlug*1, L. van Dussen1,
J.H. Runge2, M.W. Tanck3, E. Endert4, A.C. Heijboer5, E.
Fliers1, C.E. Hollak1, E.M. Akkerman2, P.H. Bisschop1
1
AMC/UvA, Endocrinology and Metabolism 2AMC/UvA,
Radiology 3AMC/UvA, Clinical Epidemiology, Biostatistics and
Bioinformatics 4AMC/UvA Clinical Chemistry, Laboratory of
Endocrinology 5VUMC Clinical Chemistry, Endocrine
Laboratory
*These authors contributed equally
p. 15
15.00-15.10
Identification of CLIC3 as a novel gene affecting bone
p. 15
formation
Andrea Brum1, Cindy van der Leije1, Jeroen van de Peppel1,
Mark Janssen2, Marco Eijken2, Hans van Leeuwen1 and Bram
van der Eerden1
1
Erasmus MC, Internal Medicine, Rotterdam, 2Arcarios BV
15.10-15.20
Characterization of CD24 expression in osteogenic
differentiating human mesenchymal stem cells
Adriana A.Matos1, T. Strini1, G. Schaaf2, W. Verschoor1, J.
van de Peppel1, J.P.T.M. van Leeuwen1
1
Erasmus MC, Department of Internal Medicine, 2Erasmus
MC, Department of Pediatrics
15.20-15.30
Predictors of new fragility fractures after diagnosis of
p. 16
indolent systemic mastocytosis
Eveline van der Veer1, S. Arends, S. van der Hoek, J.B.
Versluijs, J.G.R. de Monchy, J.N.G. Oude Elberink, and J.J.
van Doormaal.
University of Groningen, University Medical Center Groningen
15.30-15.40
Amelogenins as potential buffer during secretory stage
amelogenesis
Jing Guo1, Lyaruu D.M1, Takano Y2, Gibson CW 3, Bervoets
TJM1, van Ruijven L4, Den Besten PK5 and Bronckers ALJJ1
1
ACTA-VU, Oral Cell Biology, 2TMDU, Biostructural Science,
3
UPSDM, Anatomy and Cell Biology, 4ACTA-VU, Functional
Anatomy, 5UCSF, Oral Sciences
p. 17
15.40-15.50
Microindentation in vivo captures elements of bone
fragility independently of bone mineral density
Frank Malgo, N.A.T Hamdy, S.E. Papapoulos, N.M.
Appelman-Dijkstra
Center for Bone Quality, Leiden University Medical Center,
Leiden
p. 17
6
p. 16
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15.50-16.25
Coffee/tea break
16:25-17:15
Key-note lecture:
Targeting the osteoblast to improve bone health in type 2
diabetes
Martina Rauner
Technical University of Dresden, Germany
17.15-18.15
Drinks
18.15-20.00
Dinner
20.00-21.00
Business meeting
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Thursday, November 13, 2014
07.30-09.00
Breakfast
08.00-09.00
Registration
SESSION III
Chair: Frank Malgo
09.00-09.10
Optimal serum 25(OH)D concentrations with respect to
different outcomes
Evelien Sohl1, R.T. de Jongh2, M.W. Heymans1, N.M. van
Schoor1, P. Lips2
1
VUmc, Epidemiology and Biostatistics, 2VUmc, Internal
Medicine, endocrine section
p. 18
09.10-09.20
Refixation of loosened hip prostheses: osteogenic
capacity of periprosthetic tissue cells
Monique A.E. Schoeman1, K.E. de Rooij2, 3, A.E. Oostlander1,
C.W.G.M. Löwik2, E.R. Valstar1, R.G.H.H. Nelissen1
1
LUMC, Dept. of Orthopedic Surgery, 2 LUMC, Dept. of
Radiology, 3 Percuros BV, Enschede
p. 18
09.20-09.30
Phosphate levels are related to all-cause, cardiovascular
and COPD mortality in men: The Rotterdam Study
Natalia Campos-Obando 1, L. Lahousse 2,3, G.G. Brusselle,
A. Hofman 2, F. Rivadeneira 1,2, B.H. Stricker 2, , O.H. Franco
2
, A.G. Uitterlinden 1,2, M.C. Zillikens 1
1
Erasmus MC, Internal Medicine, 2 Erasmus MC,
Epidemiology, 3 Ghent University, Respiratory Medicine
p. 19
09.30-09.40
Stimulation of protein synthesis by enhancing mRNA
translation rate in osteoblasts
Astrid D.Bakker1, T.Gakes1, P.Juffer1, G.M.J. de Wit2,
J.M.A.Hogervorst1, J.Klein-Nulend1, R.T.Jaspers2
1
ACTA-VU, Oral Cell Biology, 2VU, FBW
p. 19
09.40-10.30
Key-note lecture:
Fundamental and clinical aspects of hypophosphatasia
Dr. Franz Jakob
University of Wurzburg, Germany
10.30-11.00
Coffee/tea break
SESSION IV
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Chair: Andrea Brum
11.00-11.10
Pitfalls in the interpretation of bone turnover markers in
liver transplantation
Charlotte G. Krol1, 5, D.C. Meiland1, O.M. Dekkers2, E. van der
Veer, H.M. Kroon3, A.J. Rabelink1, B. van Hoek4, N.A.
Hamdy1, 5
Leiden University Medical Centre, Leiden, Departments of
Endocrinology & Metabolic Diseases1, Clinical Epidemiology2,
Radiology3, Gastroenterology & Hepatology4 and Centre for
Bone Quality5; Groningen University Medical Centre, the
Netherlands; Department of Laboratory Medicine, 6
p. 20
11.10-11.20
Nbce1 (Slc4a4): a potential pH regulator during enamel
development in the mouse
Rozita Jalali1, J. Guo1, B. Zandieh-Doulabi1, TJM. Bervoets1,
RS . LaCruz2, W. Boron3, M. Parker3, P. Den Besten4 and
ALJJ. Bronckers1
1
Dep Oral Cell Biology, ACTA, University of Amsterdam and
VU University Amsterdam, MOVE Research Institute,
Amsterdam; 2 Centre for Craniofacial Molecular
Biology,School of Dentistry Los Angeles, California USA; 3
Dep Physiology and Biophysics Case Western Reserve
University, School of Medical Sciences, Cleveland OH USA; 4
Dept Orofacial Sciences, University of California San
Francisco, CA, USA
p. 20
11.20-11.30
Different human osteoclast precursors respond in
distinct ways to IL-17A
Sara Sprangers, T. Schoenmaker, Y. Cao, V. Everts, T.J. de
Vries
Oral Cell Biology and Periodontology, ACTA-UvA, VU,
Amsterdam, MOVE Research Institute
p. 21
11.30-11.40
MUC1 in osteoblasts balances osteogenesis and
angiogenesis in hypoxia
Jyotirmaya Behera, Andrea Brum, Marijke SchreudersKoedam, Johannes van Leeuwen and Bram van der Eerden
Erasmus MC, Internal Medicine, Rotterdam
p. 21
11.40-11.50
Human mesenchymal stem cell (MSC)-derived osteoblast
mineralisation depends on endogenous production of
leptin
Bram CJ van der Eerden, Marijke Schreuders-Koedam,
Yolande de Lege, Solveig Staurland, Jeroen van de Peppel
and Johannes PTM van Leeuwen
Erasmus MC, Internal Medicine, Rotterdam
p. 22
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11.50-12.00
Foreign body giant cells do not have the capacity to
resorb bone
Bas ten Harkel1, T. Schoenmaker2, T.J. de Vries2 and V.
Everts1
Oral Cell Biology1 and Periodontology2, ACTA, Oral Cell
Biology, University of Amsterdam, VU University Amsterdam,
MOVE Research Institute. Funding by NIRM.
12.00-13.00
Lunch
p. 22
SESSION V
Chair: Bas ten Harkel
13.00-13.10
TNF-α antagonist infliximab inhibits osteoclast formation
of peripheral blood mononuclear cells but does not affect
periodontal ligament fibroblast mediated osteoclast
formation
Teun J. de Vries1, J. Yousovich1, T. Schoenmaker1, N.
Scheres2, V. Everts3
ACTA, UvA and VU University, MOVE Research Institute,
1
Periodontology, 2Preventive Dentistry and 3Oral Cell Biology,
Amsterdam
p. 23
13.10-13.20
The three types of cartilage of the temporomandibular
joint respond differently to IL-1β
Hessam Tabeian1, A.D. Bakker1, T.J. de Vries2, F. Lobbezoo3,
V. Everts1.
Depts. of 1Oral Cell Biology, 2Periodontology, 3Oral
Kinesiology, ACTA-UvA&VU, MOVE Research Institute,
Amsterdam.
p. 23
13.20-13.30
Osteoblast-secreted extracellular vesicles stimulate the
expansion of CD34+ human umbilical cord blood cells
Jess Morhayim1, J. van de Peppel1, E. Braakman2, A. van
Wijnen3, J. Cornelissen2, and H. van Leeuwen1
Departments of 1Internal Medicine and 2Hematology,
Erasmus MC, Rotterdam, 3 Mayo Clinic, Rochester, MN, USA
13.30-13.40
Vitamin D reduces metastatic prostate cancer cell growth
directly and indirectly via stimulation of osteoblast
differentiation
Marjolein van Driel1, I.J. Robbesom1, L. van den Berk1, H.
Chiba2, J.P.T.M van Leeuwen1
1
Department of Internal medicine, Erasmus MC, Rotterdam,
2
Department of Basic Pathology, Fukushima Medical
University, Fukushima, Japan
p. 24
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p. 24
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IL-1β differently affects osteoclastogenesis of distinct
osteoclast precursor subsets
Yixuan Cao1, I.D.C.Jansen2, S.L.Sprangers1, V.Everts1,
T.J.de Vries2
ACTA-UvA and VU University, Oral Cell Biology1 and
Periodontology2, Amsterdam, MOVE Research Institute
p. 25
13.50-14.00
Vitamin D supplementation to prevent depression and
poor physical function in older adults: The D-Vitaal Study
Elisa J. de Koning1,2, N.M. van Schoor1,2, H.W.J. van
Marwijk1,3, P.J.M. Elders3, B.W.J.H. Penninx1,4, P.T.A.M. Lips5
1
VUmc, Dpt. of Epidemiology & Biostatistics; 2 VUmc,
EMGO+ Institute for Health and Care Research; 3 VUmc, Dpt.
Of General Practice and Elderly Care Medicine; 4 VUmc, Dpt.
Of Psychiatry / GGZ inGeest; 5 VUmc, Dpt. Of Internal
Medicine and Endocrinology
p. 25
14.00-14.10
Devitalized extracellular matrix produced by osteoblasts
accelerates osteogenic differentiation of mesenchymal
stromal cells
Marta Baroncelli1, R.D.A.M. Alves1, J.A.A. Demmers2, J. van
de Peppel1, J.P.T.M. van Leeuwen1
1
Erasmus MC, Internal Medicine, 2Erasmus MC, Proteomics
Center
p. 26
14.10-14.20
Increased osteogenic potential of sub-dermal fibroblast
from FOP patients
Elise M. Voermans1,2, D. Micha1, H.W. van Essen2, C.N.
Netelenbos3, G. Pals1, E.M.W. Eekhoff3, N. Bravenboer2
1
VUmc, Clinical Genetics, 2 VUmc, Clinical Chemistry, 3
VUmc, Internal Medicine
p. 26
14.20-14.30
Gene expression profiling of the canine growth plate
providing new insights in endochondral bone formation
Michelle Teunissen1, F.M. Riemers1, M.J.A. GrootKoerkamp2, L.C. Penning1, J. Alblas3, B.P. Meij1, M.A.
Tryfonidou1
1
UU, Veterinary Medicine, Clinical Sciences of Companion
Animals, 2UMCU, Molecular Cancer Research, 3UMCU,
Orthopaedics
p. 27
14.30-14.40
Inhibition of GSK3 stimulates BMP signaling and
decreases SOST expression which results in enhanced
osteoblast differentiation
Monique A.E. Schoeman1, M.J.C. Moester2, E.R. Valstar1,
R.G.H.H. Nelissen1, C.W.G.M. Löwik2, K.E. de Rooij2,3
1
LUMC, Dept. of Orthopedic Surgery, 2 LUMC, Dept. of
Radiology, 3 Percuros BV, Enschede
p. 27
14.40-15.00
Coffee/tea break
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15.00-15.20
Awards
15.20
Adjournment
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