Anti-CD11b antibody [M1/70] ab8878 Overview Datasheet Product

Product Datasheet
Anti-CD11b antibody [M1/70] ab8878
10 Abreviews
18 References
3 Images
Overview
Product name
Anti-CD11b antibody [M1/70]
Description
Rat monoclonal [M1/70] to CD11b
Tested applications
ICC/IF, Flow Cyt
Species reactivity
Reacts with: Mouse, Rabbit, Human
Immunogen
The hybridoma was formed by the fusion of mouse myeloma NS1 cellswith spleen cells from rats immunized with
B10 mouse spleen cells enrichedfor T lymphocytes.
General notes
CD11b is also known as MAC-1alpha, CR3 and integrin alpha M. We do not batch test ab8878 in IHC, however,
some customers have successfully used this antibody in this application. We cannot recommend this antibody
for IHC.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Purity
Protein G purified
Clonality
Monoclonal
Clone number
M1/70
Myeloma
NS1
Isotype
IgG2b
Light chain type
unknown
Applications
Our Abpromise guarantee covers the use of ab8878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 5 µg/ml.
Flow Cyt
Use at an assay dependent concentration. Each labeling step was carried out for 30 min at 0-4°C in
mediumcontaining 0.01 M NaN3. Cells (5 x 107/ml) were incubated with 50 µl M1 monoclonal antibody or
irrelevant monoclonal antibody, R4/18.2, as control in the first step, washed, suspended in 50 µl of FITC-F
(ab')2 anti-rat IgGin the second step, and washed through a layer of fetal calf serum. Can also be used in
cytotoxicity and binding assays.
Target
Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and
granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the
receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in
C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2
peptides of fibrinogen gamma chain.
Tissue specificity
Predominantly expressed in monocytes and granulocytes.
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6
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Product Datasheet
(SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile
multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal
membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
Cellular localization
Membrane.
Anti-CD11b antibody [M1/70] images
ICC/IF image of ab8878 stained Raw 246.7 cells. The
cells were 4% formaldehyde fixed (10 min) and then
incubated in 1%BSA/ 10% normal goat serum / 0.3M
glycine in 0.1% PBS-Tween for 1h to permeabilise the
cells and block non-specific protein-protein interactions.
The cells were then incubated with the antibody ab8878 at
5µg/ml overnight at +4°C. The secondary antibody
(green) was DyLight® 488 goat anti- rat IgG (H+L) used at
a 1/1000 dilution for 1h. Alexa Fluor® 594 WGAwas used
Immunocytochemistry/ Immunofluorescence - AntiCD11b antibody [M1/70] (ab8878)
to label plasma membranes (red) at a 1/200 dilution for
1h. DAPI was used to stain the cell nuclei (blue) at a
concentration of 1.43µM.
Overlay histogram showing Human macrophages
stained with ab8878 (blue line). Cells were incubated with
human immunoglobulin for 30 min at 4°C to block nonspecific protein-protein interactions. The cells were then
incubated with the antibody (ab8878, 20 µg/mL) for 30
min at 4ºC. The secondary antibody used was a mouse
anti-rat FITC (IgG2b gamma chain) (ab99671) at 1
µg/mL for 30 min at 4ºC. Isotype control antibody (black
line) was Rat IgG2b [RTK4530] (ab18541) used under the
same conditions.
Flow Cytometry - Anti-CD11b [M1/70] antibody
(ab8878)
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Product Datasheet
Overlay histogram showing Human
macrophages stained with ab8878 (red line). Cells were
pre-incubated in Human AB serum (10%) for 20 mins at
4ºC. Cells were then incubated with the antibody (ab8878,
0.1µg/1x10^6 cells) for 30 min at 4ºC. The secondary
antibody used was a goat anti-rat Alexa Fluor® 488 (IgG
H+L) (ab150165) at 1/2000 dilution for 30 min at 4ºC.
Isotype control antibody (black line) was rat IgG2b
[RTK4530] (ab18541, 1µg/1x10^6 cells) used under the
same conditions. Unlabelled sample (blue line) was also
Flow Cytometry - Anti-CD11b [M1/70] antibody
used as a control. Acquisition of >5,000 events were
(ab8878)
collected using a 20mWArgon ion laser (488nm) and
525/30 bandpass filter.
Please note: All products are "FOR RESEARCH USE ONLYAND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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