Sample Guidelines - IDEXX Laboratories

Sample Guidelines
IDEXX Quality Starts with Quality Samples
The quality and accuracy of results are very important
to everyone at IDEXX
IDEXX Tips for obtaining high quality samples:
Choose the correct sample and container
Read the collection guidelines on the following pages or in the Sample Requirements
column by each test in the Reference laboratory section of the current edition of the
Directory of Products and Services (call 00800 1234 33 99 if you cannot find the test
you require) before taking a sample. Improper choice of a collection vial can adversely
affect results.
Did You Know? Using a serum gel tube is an easy way to collect a good serum
sample for chemistry panels. However, for certain specialised tests, such as many
endocrinology and drug tests, the gel can interfere with results.
Did You Know? Whole blood EDTA samples start to degrade as soon as the blood is
outside of the animal.
To preserve cell morphology, include air-dried, unstained blood slides along with
the EDTA tube. Slides should also be included with samples submitted for fluid
analysis for accurate cytologic interpretation. Blood smears made at the time of blood
collection help avoid platelet clumping problems and allow more accurate platelet
interpretation.
Did You Know? Including slides with your Comprehensive Haematology can help
with pathologist reviews of unusual cells, and with the identification of red blood
cell parasites.
Use proper techniques
Filling syringes: When filling syringes, aim for a good free flowing stick from the
largest accessible vein. Slow draws and difficult sticks can rupture red cells, adversely
affecting CBC results and certain chemistries. The longer the blood stays in the
syringe, the greater the risk of clumped platelets and clots that degrade test results.
Always fill the tubes without additives first. This prevents carry-over of tube additives.
For example, if you are filling an EDTA tube and a serum gel tube, always fill the gel
tube first. Even a small amount of EDTA can interfere with many chemistry results.
Fill EDTA or citrate tubes precisely. Overfilling and under-filling tubes causes the wrong
ratio of additives. Excess EDTA in an under-filled tube will give inaccurate CBC results.
Overfilling the EDTA tube may cause the sample to clot.
Centrifuging: Make sure samples are fully clotted before tubes are centrifuged.
Note that some samples may take longer to clot than others.
Did You Know? In tests that require serum, it is important to collect and centrifuge
the sample properly. If a tube is spun too soon after drawing the blood, you will send
plasma to the laboratory and not serum.
If you have any questions regarding submission of specimens, or if you require
appropriate supplies, please call us on 00800 1234 33 99.
Samples clotted during blood
draw may result in…
• Platelet clumps
• Falsely decreased cell counts
(platelets, red blood cells and
white blood cells)
• Haemolysis
(when forcing blood into tube)
Excess anticoagulant
(under-filled tube) may result in…
• Decreased RBC count and HCT
due to dilution
• Altered cell morphology
• Inaccurate MCV, MCH, MCHC
and HGB
• Falsely prolonged clotting times
EDTA contamination may cause …
• Falsely decreased calcium
• Falsely increased potassium
• Interference with many
specialised tests
Specimen Collection Guidelines.... 2 – 7
Sample Submission Forms.......... 8 – 10
IDEXX Quality System........................ 11
1
Specimen Collection Guidelines
Specimen Collection Guidelines
Type of Testing
Specimen
Container
Contents
Protocol
Storage
Chemistry,
Immunology,
Endocrinology
Serum
Serum Gel Tube
Gel to separate serum from clot (during centrifugation)
and a clot activator
Let specimen clot 15 – 20 minutes,
centrifuge at 2,500 rpm for 10 – 15 minutes.
DO NOT use serum gel tubes for therapeutic drug
monitoring (digoxin, phenobarbital), or Troponin HS I
Refrigerate
Normal Flora, Predictable Susceptibility Patterns and Non-pathogenic Organisms
IDEXX follows guidelines set by CLSI, combined with our years of experience in
performing susceptibility testing. We believe these are “best practice” microbiology
techniques, and would be happy to discuss the following policies with you.
Chemistry (including
Troponin HS I), Immunology, Endocrinology
including progesterone
and therapeutic drugMonitoring (Digoxin,
Phenobarbital)
Serum
No additives
(empty/sterile)
Let specimen clot 15 – 20 minutes,
centrifuge at 2,500 rpm for 10 – 15 minutes,
remove serum and transfer to a plain serum tube
Refrigerate
Sterile Tubes
Use glass or plastic tubes with no additives. Serum Gel tubes with clot activator are not
acceptable for cultures because the clot activator binds bacteria, which inhibits growth.
Chemistry
Cardiopet® proBNP
0.5 ml
separated
EDTA plasma
Serum Tube
Plain tube
No additives
(empty/sterile)
Collect specimen in an EDTA tube, Centrifuge sample
tubes (10 minutes at 3000 rpm or StatSpin VT hard
cycle). Use a pipette to transfer plasma to plain sample
tube labelled BPNE Canine/Feline.
NOTE: The Cardiopet® proBNP assay is stable for 48
hours at room temperature. Please submit samples by
courier or Guaranteed Mail. If you anticipate a delay in
transportation, please submit samples cooled on ice.
Keep frozen
Refrigerate
DO NOT freeze
Whole blood
EDTA Tube
and air-dried
unstained slides
Anticoagulant
EDTA
Filling tube to the line mark on the tube will allow
to obtain the correct blood to anticoagulant ratio
Invert gently several times after filling
Coagulation
(PT, APTT, fibrinogen,
D Dimer,
AntiThrombin III,
Factor VII)
Citrated
Plasma
Citrate Tube
Anticoagulant
Sodium citrate
Keep frozen
Correct blood-to-anticoagulant ratio is very important.
Take blood sample from animal. At least 2 ml of blood
is required to run PT and APTT (yield minimum of 1 ml
citrated plasma). Remove needle and fill sample tubes in
the appropriate order and to the level indicated on each
tube. Mix anti-coagulated tubes. Ensure the coagulation
tubes are not clotted. If they are, repeat sampling is required. Centrifuge coagulation sample tubes (10 minutes
at 3000 rpm or StatSpin VT hard cycle). Use a pipette
to transfer plasma to sample tube labelled CITRATE
PLASMA. Please remember to include an EDTA sample
for platelet count, which is part of our coagulation profile.
To ensure the best results, please submit the citrated
plasma samples frozen, packed with ice packs to prevent
thawing, by overnight delivery to the laboratory.
NOTE: Samples that are clotted or haemolysed will not
be analysed because they would fail to provide accurate
results
See Avian and Exotic specimen submission guidelines
in this section. Check individual test listing.
Urinalysis,
Urine Culture
Urine
(Sterile
collection
preferred)
Plain urine
container
Boric urine
container
No additives
Boric Acid
After collection, cap container.
Refrigerate
and prevent
UV/sunlight
exposure
Urolith Analysis
Urolith
Dry sterile
container
No additives
(empty/sterile)
Do not place into formalin or other liquid as
stones may dissolve.
Room
temperature
Microbiology
See Microbiology Specimen Submission Guidelines
Cytology
See Cytology Specimen Submission Guidelines
Biopsy
See Histopathology Specimen Submission Guidelines
Don't Forget
Label all tubes and slides submitted with: Animals Name, Owners Name, Date of Sampling and Time of Sampling
2
Fluids
Make sure all collection devices containing fluids are sealed and leak proof before
submitting. Note: Specimens that are >48 hours old are not suitable for culture, and
loss of viability should be expected.
Blood Culture
Aerobic and anaerobic cultures are performed on all blood cultures.
Haematology
Avian Blood and Miscellaneous chemistries
Microbiology
Fluids: urine and tissue can be submitted in sterile containers (moisten tissue samples
with sterile saline or water to prevent drying and loss of viability).
Type of
Source
Collection
Device
Specimen Preparation
and Collection
Abscess
or Wound
Transport Swab
Aseptically prepare collection site. Aspirate fluid or
Aerobic and
pus from pustules or vesicular wounds and abscesses. Anaerobic Culture
Blood
2 – 5 ml for
small animal
10 ml for large
animal
Wash venipuncture site with antiseptic soap; sterile
water rinse. Apply 1 – 2% tincture of iodine or
povidone-iodine; let dry. Remove iodine with 70%
alcohol wash. Draw up to 10 mL into sterile syringe.
Change needle and inoculate a blood culture bottle
and mix well. Leave at room temperature.
Blood Culture
Bone
Marrow
Plain sterile
container
Aseptically prepare collection site.
Aerobic and
Anaerobic Culture
Central Ner- Plain sterile
vous System container
Collect CSF fluid by an aseptic subdural tap,
ventricular aspiration or lumbar puncture.
Aerobic and
Anaerobic Culture
Ears
Transport Swab
Note: Posterior pharyngeal cultures may also reveal
organisms causing otitis media.
Aerobic Culture
Anaerobic Culture
Eyes
Transport Swab
Aerobic Culture
Use swab to collect suppurative material from
cul-de-sac or medial canthus.
Note: Topical anaesthetic may inhibit bacterial growth.
Faeces
In sterile
container
Transport Swab
Avoid contamination with urine and soil.
Faecal Culture
Nail, Skin or Plain sterile
Hair Culture container
(Fungal)
Sinus
Fungal and/or
Use sterile blade or swab to collect material from
infected nail. Please do not include the scalpel blade Aerobic Culture
in the container. Swab or scrape active border of skin
lesions. Use an envelope for hair.
Sinus
Aspirate from maxillary, frontal or other sinuses.
Note: Chronic sinusitis often involves anaerobic
bacteria.
Aerobic and
Anaerobic Culture
Plain sterile
container
Please DO NOT submit
syringes with needles.
Test to
Request
Tissue
Plain sterile
container
Place tissue in a plain sterile container with small
amount of sterile saline to keep specimen hydrated.
Aerobic and
Anaerobic Culture
Urine*
Plain sterile
container
Boric acid
container
Cystocentesis is strongly recommended
(except in large animals).
Avoid contamination with faeces. Keep refrigerated.
Indicate collection method.
Urine Culture

If you have any questions regarding
submission of specimens or require
supplies call us on 00800 1234 33 99
*Cystocentesis samples: Either plain or boric
samples are suitable for culture.
For all other urine samples boric acid samples
are recommended if culture is required.
3
Specimen Collection Guidelines
Cytology
ACCURATE RESULTS DEPEND ON QUALITY SPECIMENS. PLEASE FOLLOW
THESE GUIDELINES:
ACCURATE RESULTS DEPEND ON QUALITY SPECIMENS. PLEASE FOLLOW
THESE GUIDELINES:
Perform sampling by fine-needle aspiration or non-aspiration biopsy, scrapings or
imprints. Prepare slides in-clinic using either a “squash” preparation or blood-smear
technique.
Call if you have questions about slide preparation.
Please DO NOT submit syringes with needles.
Turnaround Time
Most evaluations will be completed within 3 – 4 days of receipt in our laboratory
(unless otherwise indicated). Additional fixation or decalcification will take longer. We
will notify you if an unusually long delay is anticipated.
Patient history and clinical findings contribute to an accurate result.
On containers and slides, please write:
• Patient’s name
• Site/Source
On the requisition form, include:
• Patient details (owner‘s name, patient‘s name, age, sex, species, breed, etc.)
• Reference to any previous laboratory results (FBC, biochemistry profile, prior cytology/
histology or serology) be sure to include our laboratory reference numbers
• Gross lesion description
• Specific anatomic location (eg., cutaneous, subcutaneous, deep tissue, intrathoracic, intra-abdominal)
• Size, shape, consistency, symmetry, definition of borders
• Clinical history – duration of lesion, progression of lesion, treatment and response
to therapy
• Radiographic and ultrasonographic findings
When submitting aspirates and impressions:
• Submit one to three un-stained air-dried slides
• Store at room temperature
• DO NOT spray with hairspray or other fixatives
• DO NOT expose to formalin fumes
• DO NOT ship slides for cytology in the same bag as a formalin-containing
biopsy jar.
When submitting fluids and washes:
• Enclose fluid in a plain EDTA Tube along with unstained air-dried slides.
• Prepare slides immediately after fluid collection to preserve cell morphology (most
fluids are stable for only a few hours at room temperature).
• If volume allows, also supply fixed fluid in a EDTA Tube by adding two drops of 10%
formalin saline (as supplied in our histology pot) per ml of collected fluid. Formalin
fixation may improve cell preservation in fluids and is particularly indicated for the
evaluation of cerebro-spinal fluid, respiratory specimens, and urine.
• DO NOT submit fluids in a gel tube, in a syringe, or as cover-slipped and wet
preparations. Submission of fluid in a gel tube can interfere with accurate cytologic
evaluation due to the presence of clotting activators.
When submitting urine samples:
• Please do not submit sedistain urine slides as these dry out and are non-diagnostic
4
Specimen Collection Guidelines
Collection Technique
Samples are collected for histological examination by standard surgical techniques or
at post mortem examination.
Histopathology
Labelling Criteria
Please ensure all specimen containers are labelled with
• Patients name, date
• Type of specimen, (Site / Source)
Requisition Information
A thorough clinical history and details of the physical examination are essential for
the correct histological interpretation of tissue changes. Information required includes
details (species, breed, age, sex), a description of the appearance and distribution
of lesions, duration of the condition, biopsy sites or post mortem tissue, response to
prior treatments, current treatment regimes and any other relevant information.
You may include any questions to be answered on your requisition form.
Fixation Guidelines
Tissue samples should be fixed in 10% buffered formalin.
Did You Know? The 10:10:10 Rule? For optimum fixation and sectioning use 10%
formalin; 10:1 ratio; and a biopsy size 10mm cubed
• Place specimens in a biopsy pot supplied by IDEXX Laboratories, with the ratio of
formalin to tissue > 10:1.
• Samples that are very tiny (less than 2mm in diameter) and friable, haemorrhagic,
mucoid or fatty in nature may not be suitable for histology processing and
interpretation. If a biopsy of this size or nature is received the lab will proceed
with testing but inform you if the sample submitted is potentially inadequate. If the
sample is inadequate you will be informed via a preliminary report, that the tissue
did not survive processing or is not diagnostically viable, upon which the charge will
be removed.
• With the exception of biopsies, if submitting multiple samples please use a separate
pot for each specimen and label each with anatomical site. If samples are to be
placed together, please clearly indicate this on the request form, and refrain from
small samples (under 1cm) being submitted in the same container as larger ones.
• Submit entire lesions and tumours with adjacent excised tissue.
• For rapid fixation of larger lesions and tumours, cut a section 0.5-1cm wide through
the centre of the specimen. Where possible, cut the skin surface, so the deep
surgical margin is not cut.
• Open hollow organs, such as intestine, prior to placing them in fixative.
• Small fragile specimens (bone marrow, Tru-cut liver or kidney) can be submitted in
mesh cassettes, available from our supplies department.
• Please do not submit needles with samples. Large specimens can be labelled with
suture material.
• Samples placed on card often fall off during transit. Smaller biopsies from different
sites can be placed in individual labelled containers to denote the site.
• High priority samples can be dispatched on the day of collection as they will fix on
their way to the laboratory. Samples of lower priority can be fixed for 24 hours at
clinic prior to dispatch to the laboratory.
Transport Guidelines
All samples should be placed in a well
sealed leak proof bag containing enough
absorbent material for the volume of
formalin.
Fixed tissue which is to be mailed may
be placed in a leak proof plastic bag
or container with a formalin soaked
gauze to keep the tissue moist (ensure
adequate fixation has occurred prior to
transportation).
Necropsy Samples
IDEXX no longer offers in-laboratory
necropsy service, but there are a variety
of options for submitting necropsy
samples. Please contact our Customer
Support team on 00800 1234 3399 for
further advice.
5
Specimen Collection Guidelines
Avian and Exotic
Specimen Collection Guidelines
Blood samples should be collected aseptically from veins, most commonly the jugular
vein in companion birds. Ulnar veins or tibiotarsal veins can be used in some species.
Nail clipping is NOT a suitable method for collecting blood samples.
The following guidelines will allow our staff to process your specimens safely,
efficiently and accurately. They will also help to ensure your samples arrive with us
in a satisfactory condition for testing.
Biochemistry Only
Submit sample in lithium heparin tubes. Gel separator tubes can be used. Gel tubes
can be spun before submission, and this will reduce storage artefacts.
Plain serum tubes can also be used, but result in a smaller sample for analysis, and
may reduce the number of measurements that can be performed.
Small sample volume may limit the number of biochemistry tests that can be
performed. If submitting a small sample, please note your required tests in order of
preference on the submission form.
Please:
• Do not use tubes or swabs that are past their expiry date.
• Do not send unlabelled tubes.
• Do not stick labels onto tubes – we need to bar code all specimens on receipt and
this is hindered by this practice.
• Do not cover the tubes with sticky tape – if the lids are screwed on tightly they will
not leak.
• Do not send hypodermic needles.
• Do not send glass tubes or slides unless suitably packaged. Slide holders are available free-of-charge from our laboratory.
• Do not send any sample unless suitably packaged – specific packaging instructions
are provided and packaging material is available from our laboratory. It is the sender’s responsibility to ensure that all pathological specimens are packed in accordance with current Royal Mail or Courier regulations. Non - adherence to packaging
instructions may result in sample return or disposal by the carrying company and
the imposition of possible financial penalties.
Haematology Only
Submit slide(s) and whole blood. Make slides immediately after collection, using
blood that has not been exposed to anticoagulant. Preferably make two slides, one by
the usual slide-and-slide method, one by a coverslip-and-slide method. Air dry slides
immediately, but do not fix or stain.
Submit the whole blood in lithium heparin. Gel separator tubes can be used but must
NOT be spun down.
EDTA can also be used for most birds (excluding ratites, crows and ravens), but is
not the preferred sample. EDTA should not be used for reptile blood, use of lithium
heparin is recommended.
Haematology and Biochemistry Required
Submit blood films (see haematology above) and blood in lithium heparin. Do NOT
spin gel separator lithium heparin tubes if only a single tube is submitted. At least
one unspun lithium heparin tube must be submitted. Small sample sizes may limit the
number of biochemistry tests that can be performed. If submitting a small sample,
please note your required tests in order of preference on the submission form.
Please note: We keep all specimens received at the laboratory for seven (7) days
after which they will be disposed of in an appropriate manner, so when the results of
your initial sample submission point to the next step in your investigation, and we have
the appropriate sample at our laboratory, simply call our Customer Support Team to
discuss your requirements.
Spoiled Samples
When a sample is received in poor condition, e.g. clotted, lipaemic, or haemolysed,
we will perform the requested tests wherever possible. Many spoiled samples can be
avoided by the use of correct tubes. It is for this reason that we provide polypropylene,
screw top tubes, free of charge. (Shipping charge of £7.50 + VAT will apply to each
dispatch)
Samples from patients on chemotherapy treatment
Please indicate clearly on the submission form if samples are from patients on chemotherapy treatment.
Cytotoxic drugs are eliminated by renal or faecal excretion within 48 hours of being
administered, and all samples require special handling in order to comply with health
and safety regulations.
Accurate results depend on quality specimens
Sample Tubes & Supplies
Sample tubes are provided free-of-charge by IDEXX Laboratories. These tubes are
designed to be used with our analysers, and use of other tubes may cause delay in
processing.
Courier & Customer
SUPPORT
Reference
Laboratories

6
Veterinary
Pathologists
All enquiries:
00800 1234 33 99
Fax:
01937 544 001
Internal Medicine
Consultant
Email:
labhelp@idexx.com
• EDTA Tubes
• Serum Tubes
• Serum Gel Tubes
• Citrate Tubes
• Heparin Tubes
• Swabs with charcoal media
• Plain Swabs (for PCR tests)
• CEM Swab
• Plain Universal Containers
• Boric Acid Universal Containers
• Faecal Universal Containers
• Histology Pots containing
10% formalin
• Endoscopy Biopsy mesh Cassettes
• Slide Containers
• Packaging Supplies
All of these tubes are supplied free of charge to our clients
(shipping charge of £7.50 + VAT for each order). To order supplies, please fax
one of our supplies order forms to 01937 544 170 or order online at idexx.co.uk.
7
Sample Dispatch Guidelines
Sample Dispatch Guidelines
Regulations for the shipment of
Pathological Samples to the Laboratory
Packaging you Samples for Dispatch
to the Reference Laboratory
• All known infectious samples must be packaged in accordance with
IATA602 in approved IARA602 infectious boxes
• Diagnostic samples must be packaged in accordance with the revised
Royal mail regulations
STEP 1 – ORDER TEST KITS
According to the IATA regulations, Infectious substances are defined as:
Infectious substances:
“Substances which are known to contain, or are reasonably expected to contain,
pathogens. Pathogens are defined as micro-organisms (including bacteria, viruses,
rickettsiae, parasites, fungi) and other agents such as prions, which can cause
disease in humans or animals.”
1. Category B Shipping Box
2. Order Form
4. Pressure Bag
5. IDEXX Overpack Bag
3. Specimen
Classification of biological products and Diagnostic Specimens:
“Those known or thought likely to contain infectious substances. Diagnostic
specimens being transported to undergo confirmatory testing must be considered to
fall within this group (see packing instructions P650)”.
You represent and warrant that any sample containing any hazardous substance
which is to be delivered to IDEXX will be packaged, labelled, transported, and
delivered in accordance with applicable laws.
Biological substances must be posted in packaging that complies with Packaging
Instruction P650 and the senders name and return address must be clearly visible
on the outer packaging. If you would like to view the comprehensive guidelines on
how to package and submit samples to the Reference Laboratory, the IATA Guidance
document is available on the Royal Mail website:
http://www.royalmail.com/sites/default/files/Guidance-Document-InfectiousSubstances-171012.pdf
STEP 2 – PREPARE BOX
1. Assemble the Category B box by
folding up the top half of the box.
The side with the two cut out holes
forms the bottom of the box.
If you wish to send samples that are known to be infectious, please ensure that the
IATA 602 regulations are met.
Please note: we are unable to accept any sample falling into Category A –
high risk to humans
2. Fold in the small side flaps so they
are hovering over the holes.
Approved IATA 602 boxes can be purchased through a number of suppliers or can be
purchased / rented at a discounted rate from IDEXX Laboratories.
The above information is a guide only and if you have any further queries please
do not hesitate to contact our Customer Support team: 00800 1234 3399 Option 1
followed by Option 1
3. Fold up the sides of the box and
lock the two tabs into place in the
notches in the base of the box.
8
9
Sample Dispatch Guidelines
STEP 3 – PREPARE SAMPLES
Sample Dispatch Guidelines
STEP 4 – SHIPPING STEPS
1. Place
the prepared Specimen bag (s) containing the
sample (s) and order form (s) into the box.
1. E
nsure you complete the appropriate Requisition Form
(remembering to include both your practice code and
practice address) and submit all sample types required.
Ensure that the sample tubes and slides are labelled with
the patient and owners names, and your practice Vet Code.
Please note: More than one specimen Biohazard bag can
be placed in one shipping container, however only one
requisition should be placed into the Biohazard bag.
2. F
old the sides of the lid into the box and push the top down.
2. E
nsure the caps are tightly screwed onto each sample
container, and wrap the tubes in a piece of absorbent
material in case of leakage. Please do not use sellotape
either on the tubes or on the packing material.
3. F
inally, fold in the side tabs and close the box
3. Place the wrapped tubes into the sealable pocket of the
supplied Specimen Transport bag, and seal by removing the
paper strip and holding the sticky tab over at the fold line
4. Now that your box is prepared, place the
box into the IDEXX Overpack bag and seal
the bag by tearing of the red strip.
4. F
old the Requisition Form with the typeface facing outwards
(as in the picture) and place in the open pocket of the Specimen
Transport bag
!
10
Following these guidelines will allow our staff to process your specimens safely, efficiently
and accurately. They will also help to ensure your samples arrive with us in a satisfactory
condition for testing.
If you would like any further advice please contact our Customer Support team:
Telephone: 00800 1234 33 99 : Fax: 01937 544 001 : e-mail: customersupportUK@idexx.com
11
Sample Submission Form
Sample Submission Form
Small Animals Request Form
Histo/Cytology Request Form
SMALL ANIMAL REQUEST FORM
IDEXX Reference Laboratories
LAB NUMBER
(LAB USE ONLY)
Please ensure you
add your VET CODE
VETERINARY SURGEON
OWNER‘S NAME
ADDRESS STAMP
OWNER‘S ADDRESS
VET CODE
e.g.
A999
date
ANIMAL ID / PIMS NUMBER
xx-xx-xxxx
Mr./Ms. Surgeon
Practice Name
Street
Town
County
Post Code
Mr./Ms. Owner
Ensure all information is
completed at the top of
Street, Town, County, Post
Code
the form
Spot
Canine
6 years
x Bred
M
NAME OF ANIMAL
SPECIES
SEX
DISCOUNT IDEXX Advantage Programme| Code: IDAV

STANDALONE TESTS
SCREENS & ADD ONS
Get the
FOUNDATIONAL
IDEXX Advantage NSAID
NSAID CLIP
Refer to the current Chem 10 Chem 10 CLIP
Directory of Products Chem 15 Chem 15 CLIP
Chem 17
Chem 17 CLIP
and Services for
LYTE 4
LYTE 4 CLIP
further information
PLEASE TICK IF YOU WISH FOR THE
FBCS/
FBCE
Full Blood Count
(Canine, Feline, Exotics, Avian)
BA
Bile Acid (Fasting)
Comprehensive Haematology
BAS
Bile Acid Stimulation Test
ACC/AFC
Coombs‘ (Canine/Feline)
FILM
Blood Film Examination
CRPR
C Reactive Protein
ACOA
Coagulation Profile
CC/FC
Coombs‘
F/CB
Cobalamin & Folate (Can./Fel.)
AT4C/AT4F
Total T4
FRU
Fructosamine
AVT4
Free T4 by immunoassay
CPLI
Spec cPL® (Canine)
BAP
PTC
Bile Acid Add a „post feed“
bile acid to profiles that
ENDOCRINOLOGY
contain a fasting bile acid
FPLI
Spec fPL® (Feline)
FFFC
Spec fPL®, Folate & Cobalamin
SFTC
Spec fPL®, Folate & Cobalamin, TLI
CBNP/F
Cardiopet® proBNP (Can./Fel.)
TNI
Troponin I HS
PCRU
TLIC/F
Protein : Creatinine Ratio (Urine)
TLI (Canine/Feline)
TFCB/
FTFC
TLI, Folate & Cobalamin
(Canine/Feline)
^
ORGAN SPECIFIC
Cardiac
Kidney Biochemistry
KPN
Liver
SLFTN
Liver plus Bile Acid
Stimulation
LFTBN
Liver & Kidney
L/KN
DIABI Diabetes Monitoring
DIABN
CANINE SPECIFIC
PANCI Vomiting Dog/Pancreatitis
PANCN
FELINE SPECIFIC
AFINI
Old, Thin Cat
Select
PANFI Under The Weather
(incl. Spec fPL®)
GENERAL
Add On!
L/KI
CARDN/FN
add on test
choice SP1N
SP1I
Basic
SP2I
Extended Basic
SP2N
SP3I
Comprehensive
SP3N
SP4I
Fits and Faints
GER1I Polyuria/Polydipsia
GERTI Geriatric incl. T4
SP4N
Bile Acid Stimulation Test
Cardiopet® proBNP
CBTP
Cardiopet® proBNP
and Troponin I HS
AELE
Electrolytes
AFRU
Fructosamine
APCR
Protein : Creatinine Ratio
ASPE
Protein Electrophoresis
(Serum)
AUCE
Urine Chemistry, Microscopy & Culture, Protein :
Crea Ratio
ACPL
Pre-anaesthetic
PASN
General Health
WELN
Spec cPL®
ACTF
Spec cPL®, Folate, Cob., TLI
ATFC
TLI, Folate, Cobalamin
ACRP
C Reactive Protein
AFPL
PLEASE TICK IF YOU WISH FOR THE SCREEN^
TO BE NON INTERPRETED
COAG
Coagulation Profile
PT & PTT
THYROID
CTPV
FTPV
Canine Thyroid Panel
Feline Thyroid Panel
T4CM/
T4FM
Total T4
CTSH
Canine TSH and Total T4
INFECTIOUS DISEASES
VFT4
Free T4 by immunoassay
INFECTIOUS DISEASE FELINE
FT4R
Free T4 (Equilibrium Dialysis)
FeLV Antigen
FIV
FIV Antibody
ACTH/V ACTH Stimulation
FACT
FF
FeLV and FIV
FIP
Feline Coronavirus (FIP) Antibody
CORT
Cortisol (basal)
TOXO
Toxoplasma IgG/IgM
DEXL
Dexamethasone Suppression (Low)
MPCR
Mycoplasma haemofelis/
Cand. Mycoplasma haemominutum/
Cand. Mycoplasma turicensis DNA PCR
(Haemobartonella DNA)
PGT
Progesterone
OTHER HORMONES
Spec fPL®
IN
Insulin (Canine)
G_ALSC Screen (Can. & Fel.)
G_ALI
Indoor Panel
Spec fPL®, Folate & Cob., TLI
AFCS
Spec fPL®, Folate & Cob.
ATFF
TLI, Folate & Cobalamin
T36P
Vetoryl Profile
AFF
FeLV/FIV
DIG
Digoxin
OTHER ASSAYS REQUIRED
AMPC
Haemoplasma RealPCR
PHEN
Phenobarbitone
Please specify:
KBR
Potassium Bromide
PPBR
Combined Phen & KBr
PLFT
Phenobarbitone Profile
CROSS REF. N°
THERAPEUTIC MONITORING
species
age
breed
sex
6 years
neutered
entire
M
Please see directory of services for content and sample requirements.
UHIS
FastTrack Histology
HISK
Histology & Complete skin Exam
HIST1
Histology 1 site
HIST4
Histology 4 sites
HICY
Histology & Fluid Cytology
HIST2
Histology 2 sites
HISA
# of sites above 4: ………………
HISM
Histology & Cytology (FNA)
HICL
Histology & Culture
Tick this box if you HIST3
Histology 3 sites
would like your sample
CYtOLOgY* Please see directory of services for content and sample requirements.
processed urgently
FLUID CYtOLOgY (includes microbiology)
NON-FLUID CYtOLOgY (only smears submitted)
(Additional charge of
ASP
CYTO1
Miscellaneous Fluid Aspirate
Cytology 1 site
£12.70 will be applied)
WASH/LAVAgE/FLUSH (includes microbiology)
TRAW
tracheal Wash
ASC
Abdominal Fluid
CYTO2
Cytology 2 sites
BAL
Bronchioalveolar Lavage
PF
Pleural Fluid
CYTO3
Cytology 3 sites
NW
Nasal Wash/Flush
PERI
Pericardial Fluid
CYTO4
Cytology 4 sites
PRW
Prostatic Wash
SYNO
Synovial Fluid
CYTA
# of sites above 4: ………………
FFCY
Fixed Fluid Cytology
CSF
Cerebro-Spinal Fluid
LNAS
Lymph Node Aspirate
UCYT
Urine Cytology (no culture)
SWCY
FNA & Culture/Sensitivity
UCEC
Urine Complete Exam
BMEX
Bone Marrow (includes FBC)
Don’t forget to select
the test(s) required
* Fees are determined by the number of anatomical sites/lesions sampled. Please refer to our Directory of Services for further details regarding sampling and fees. HIStOLOgY – The same standard charge is applied to submissions
obtained from up to 2 sites (HISTO1 and HISTO2). The fee increases for submissions from 3 or more sites. Up to 4 biopsies from inflammatory skin disease, multiple lymph nodes, or endoscopic biopsies are classified as a single
anatomical site. CYtOLOgY – The same standard charge is applied to submissions obtained from up to 2 sites (CYTO1 and CYTO2). FNAs from multiple lymph nodes are classified as a single anatomical site. The fee increases for
submissions from 3 or more sites. Please note that for fluid and wash/lavage/flush cytology, each additional site is charged at an incremental fee equal to 50% of the standard charge.
HIStORY, SItE, AND LESION DESCRIPtION:
ALLERGY ASSESSMENT PROGRAMME
AFTC
TM
Don’t forget to select
the test(s) required
FELV
ADRENAL/PITUITARY
REPRODUCTION
 FELINE SPECIFIC ADD ONS
GERTN
WELI
PREVIOUS REF. N°
ABAS
 CANINE SPECIFIC
GER1N
PASI
COAGULATION
ABNP/F
AFINN
PANFN
BIOCHEMISTRY
AFBC
 GENERAL
SCREEN TO BE INTERPRETED
KPI
Select interpreted or
SLFTI
non interpreted option
LFTBI
HAEMATOLOGY
 ADD ON TO ANY SCREEN
A999
Ensure all information is
completed at the top of
the form
name of animal
courier or lockbox sticker
vet code
HIStOLOgY*
Mr./Ms. Owner
Street, Town,
County, Post Code
Spot
Canine
x Bred
owner‘s address
Practice Name
Street
Town
County
Post Code
Please ensure you
add your VET CODE
e.g.
owner‘s name
practice address stamp
lab number
(lab use onlY)
ENTIRE
See price list for profile content & sample requirements.
Please tick test required ü
Please tick box for IDEXX Advantage discount and ensure you enclose your IDEXX Analyser result or SNAP® Test device.
Advantage prices will only be applied if in Clinic results / SNAP device are received. Tests only applicable if marked with
CARDI/FI
NEUTERED
veterinarY surgeon
xx-xx-xxxx Mr./Ms. Surgeon
AGE
BREED
HISTO/CYTOLOGY REQUEST FORM
IDEXX Reference Laboratories
© 2013 IDEXX Laboratories, Inc. All rights reserved. · UK044 as of 4-2013
DATE
Please ensure all text is legible. This helps us process your request more efficiently and also
ensures you receive appropriate interpretation if you have requested an interpreted profile.
© 2008 IDEXX Laboratories, Inc. All rights reserved. · UK043 as of 10-2011
Please ensure all text is legible. This helps us process your request more efficiently and also
ensures you receive appropriate interpretation if you have requested an interpreted profile.
Has whole tumour been submitted?
o
Yes
o No
Previous Ref Nr
dorsal
Biopsy Method
Endoscopy
Please fill out all
relevant information/
details here
Excisional
Incisional
G_ALGUK Grasses & Weeds Panel
Post Mortem
G_ALE
Other:
Trees Panel
Differential Diagnosis/Disease Suspected:
Please indicate the
site of the lesion here
Cytology type
Please check Quick Guide or Directory of
Services for further assays and codes.
FNA
Wash/Lavage
History, Clinical Findings & Current Therapy (Please indicate if continuing overleaf)
Impression
Please fill out all
relevant information/
details here if you
require interpretation
of your results
Non Fasting Sample
FOR LAB USE ONLY
EDTA
SMEAR
PI TUBE
12
SEP SERUM
UN SPUN GEL
WHOLE BLOOD
PART SPUN GEL
FLUORIDE
SPUN GEL
Analyser result/SNAP® TEST enclosed
HEPARIN
HEPARIN PLASMA
CITRATE
CITRATE PLASMA
PLAIN URINE
BORIC URINE
UNLABELLED
LOCK BOX
COURIER
GTD
REV
NHIS
Recent treatment
Cystocentesis
Other:
ventral
Tissues to be submitted in 10% formal-saline. Polypropylene tubes with fixative are available from the laboratory. Please do not push large tissues into small pots. Please ensure absorbent material is included in order to contain any leakage in transit.
FOR LAB USE ONLY
IDEXX Laboratories Ltd
Tel: 00800 1234 3399 · Fax: 01937 544001
labhelp@idexx.com · www.idexx.co.uk
Scraping
HISTOLOGY POTS
FIXED EDTA
PLAIN URINE
SMEARS
BORIC URINE
PLAIN TUBE
SWAB
LABELLED
EDTA
CULTURE BTL
UNLABELLED
CROSS REF. N°
IDEXX Laboratories Ltd
Tel: 01937 544000 · Fax: 01937 544001
labhelp@idexx.com · www.idexx.co.uk
13
Sample Submission Form
IDEXX Quality System
Microbiology Request Form
Please ensure all text is legible. This helps us process your request more efficiently and also
ensures you receive appropriate interpretation if you have requested an interpreted profile.
MICROBIOLOGY REQUEST FORM
IDEXX Reference Laboratories
LAB NumBeR
(LAB uSe ONLY)
Please ensure you
add your VET CODE
ANImAL ID / PImS NumBeR
VeTeRINARY SuRgeON
OwNeR‘S NAme
ADDReSS STAmP
OwNeR‘S ADDReSS
VeT CODe
e.g.
xx-xx-xxxx
Mr./Ms. Surgeon
Practice Name
Street
Town
County
Post Code
A999
Mr./Ms. Owner
Ensure all information is
completed at the top of
Street, Town, County, Post
Code
the form
Spot
Canine
6 years
x Bred
M
NAme Of ANImAL
SPeCIeS
Age
BReeD
Sex
DISCOUNT IDEXX Advantage Programme| Code: IDAV
è
URINE Please indicate collection method:
NATURE OF SPECIMEN OR SITE OF SWAB
Cystocentesis
Catheter
GENERAL
SW
Basic Urine Screen
Extended Basic Urine Screen
Comprehensive Urine Screen
Swab (Culture & Sentest)
Ensure you state
SWCY Cytology & Swab
the
collection
site
MRSA
MRSA/MRSP
Culture
ofCEM1
the specimen
CEM - 1 Swab
Premium Urine Screen
FAECES
FAECES
Free Catch
I do NOT wish for faecal samples to be pooled
INFECTIOUS DISEASE PANELS
HPCR
Herpesvirus FHV-1 (DNA) RealPCR™
FELINE
FEAP
Enteritis (Panleucopenia/FPV)
(DNA RealPCR™
FCOP
Feline Coronav. (FIP) (RNA)
RealPCR™
FDPP
Feline Diarrhoea Panel
FEP1
Feline Eye Panel 1
FEPC
Feline Eye Panel 1 plus bact.
CEM2
CEM - 2 Swabs
FP2
Feline Eye Panel 2
BC
Blood Culture
FP2C
Feline Eye Panel 2 plus bact.
MPCR
Feline Haemotropic Mycoplasma
(Haemobartonella felis) (DNA)
FUR1
Upper Respiratory Panel 1
SARC
Basic Faecal Screen
SC
Synovial Culture
SARO
Extended Basic Faecal Screen
FT
Fresh Tissue Culture
CFA
Comprehensive Faecal Screen
MYCO
Mycoplasma Culture
PFA
Premium Faecal Screen
TBCU
Mycobacterium Culture
ZP1
Zoonosis Package 1
ACID
Acid Fast Organisms (ZN Stain)
ZP2
Zoonosis Package 2
AVC
Basic Avian Faecal Screen
UENC
Urine Chemistry & Microscopy
AVCC
Comprehensive Avian Faecal Screen
PCRD
CDPP
Canine Diarrhoea Panel
MSH
Basic Exotic Faecal Screen
Protein : Creatinine Ratio &
Urine Deposit Microscopy
CRP1
Canine Upper Respiratory Panel
MSHC
Comprehensive Exotic Faecal Screen
UCUL
Urine Culture & Sentest
CRCP
Canine Upp. Resp. Panel plus bact.
FEA
Basic Equine Faecal Screen
UCD
Urine Deposit Microscopy
TRA1
Travel Disease Profile I
FEFC
Comprehensive Equine Faecal Screen
STOX
Stone Analysis
TRA2
Travel Disease Profile II
FEF
Equine Faecal Screen - Foal
TRA3
Travel Disease Profile III
CDIFF
C.difficile selective culture
TIC1
Tick Panel – Blood
TIC2
Tick Panel – Tick
URINE
FU1C
Upper Respiratory Panel 1 plus bact.
FUR2
Upper Respiratory Panel 2
FU2C
Upper Respiratory Panel 2 plus bact.
CANINE
FAECES
è ADD ONTO ANY FAECAL SCREEN
ATFC
TLI, Folate, Cobalamin (Can.)
FPAR
Faecal Parasitology
ATFF
TLI, Folate, Cobalamin (Feline)
BAER
Baermann's Test for Lungworm
AGIA
Giardia Antigen (ELISA)
Baermann‘s Test
for Lungworm
C.difficile selective culture
OB
Occult Blood
SALM
Salmonella
CAMP
Campylobacter
INFECTIOUS DISEASE INDIVIDUAL TESTS
SCA
Salmonella & Campylobacter
FELINE
GIAR
Giardia Antigen (ELISA)
BAEA
ACDIFF
SKIN
SCE
Basic Skin Screen
SCEF
Extended Basic Skin Screen
SCEFE
Comprehensive Skin Screen
(Equine Only)
NAIL1
Basic Nail Examination
NAIL2
Comprehensive Nail Examination
AVIAN
PREVIOUS REFERENCE NUMBER:
Avian Panel 1
AVP2
Avian Panel 2
CHLS
Chlamydia felis Antibody
Cryptosporidia Antigen
FCVS
Calicivirus Antibody
PARV
Parvovirus Antigen (CPV)
FHVS
Herpesvirus Antibody
PAN
Feline Panleucopenia (FPV) Antib.
FIMM
Immune Status
BHA
Bartonella henselae Antibody
FHW
Heartworm Antibody
FLYM
Lyme Antibody (Borrelia spp.)
FEAG
Feline Enteritis Antigen
CALF
Calicivirus (RNA) RealPCR™
CPCF
Chlamydia (DNA) RealPCR™
FCUL
Dermatophyte Culture
SPF
Skin Paras./Dermatophyte (Direct Mic.)
MFC
Skin Paras./Dermatophyte (Fungal cult.)
è ADD ON TESTS
CROSS REFERENCE NUMBER:
AVP1
CRYP
SKIN
APCR
AMSA
Protein : Creatinine Ratio
MRSA/MRSP Enrichment
eNTIRe
TOPC
Toxoplasma gondii (DNA) RealPCR™
VIF
Virus Isolation (Respiratory)
AVIAN
CPCR
Chlamydia psittaci (DNA) RealPCR™
PBFD
Psitaccine Beak & Feather Disease
ASEX
Avian Sexing
CANINE
BABC
Babesia canis Antibody
FILM
Babesia canis Film Examination
CIH
Infectious Hepatitis Antibody
Don’t
DIS
forget to select
the test(s) required
ERL
Ehrlichia canis Antibody
HW
Heartworm Antigen
LEI
Leishmania Antibody
LEPMAR
Leptospira Antibody (Full Screen)
NEO
Neospora caninum Antibody
PAR
Parvovirus Antibody (CPV)
TOXC
Toxoplasma IgG/IgM
DAPS
Vaccination Status
LEPV
Leptospira Antibody
BBPC
Babesia spp. (DNA) RealPCR™
LYPC
Lyme (Borrelia spp.) (DNA) RealPCR™
EPCR
Ehrlichia canis (DNA) RealPCR™
LPCR
Leishmania (DNA) RealPCR™
LYM
Lyme Antibody (Borrelia spp.)
ASPS
Aspergillus Antibody
DIPC
Can. Distemper CDV (RNA) RealPCR™
TOPC
Toxoplasma gondii (DNA) RealPCR™
VIC
Virus Isolation (Respiratory)
CPPC
Canine Parvovirus (DNA) RealPCR™
CPV2, CPV2a, CPV2b, CPV2c
OTHER ASSAYS REQUIRED:
Please fill out all
relevant information/
details here
FOR LAB USE ONLY
14
PLAIN fLuID
fIxeD fLuID
DRY SwAB
OTHeR
fReSH TISSue
uNLABeLLeD
LOCK BOx
COuRIeR
gTD
ReV
NHIS
Fully approved as a training laboratory
by the European College for
Veterinary Clinical Pathology (ECVCP)
Technically valid results are ensured via the use of:
•Validated testing methods, using
suitable reagents, and equipment
which is regularly calibrated, checked
and maintained to a high standard.
•Appropriately qualified and trained
staff, including technicians, veterinary
pathologists, customer support and
administration personnel.
•Full investigation into any problems
•Controlled Standard Operating
that come to light, with appropriate
Procedures (SOPs) for all test methods.
corrective action taken to prevent
reoccurrence and improve the way
•Extensive quality control programmes
that we work.
including inter-laboratory comparisons.
Distemper Virus Antibody
History, Clinical Findings & Current Therapy (Please indicate if continuing overleaf)
PLAIN uRINe
SeRum
SKIN SCRAPe
BORIC uRINe
fAeCeS
SKIN SLIDe
CHARCOAL SwAB
eDTA fLuID
eDTA
Analyser result/SNAP® TEST enclosed
A detailed list of accredited tests with associated methods and Standard Operating
Procedures is available on our website www.idexx.co.uk/ukas, and the scope for the
accreditation can be found on the UKAS website www.ukas.org/testing.
2534
Accredited by UKAS to
BS EN ISO/IEC 17025:2005
See price list for profile content & sample requirements.
Please tick test required ü
Please tick box for IDEXX Advantage discount and ensure you enclose your IDEXX Analyser result or SNAP® Test device.
Advantage prices will only be applied if in Clinic results / SNAP device are received. Tests only applicable if marked with
Get the
IDEXX AdvantageSCREENS
URINE
Refer to the current
Directory of ProductsUCE
UCEP
and Services for
UCEC
further information UCECP
NeuTeReD
© 2013 IDEXX Laboratories, Inc. All rights reserved. · UK042 as of 04-2013
DATe
Pathology services play a crucial role in ensuring accurate diagnosis and effective
treatment and monitoring for patients. Our Wetherby Laboratory gained
accreditation in 2004 by the United Kingdom Accreditation Service (UKAS) to BS
EN ISO/IEC 17025:2000 (now updated to 17025:2005) General standards for the
competence of testing and calibration laboratories and since then, with continuing
process improvement, have expanded on the number of tests included in the scope
of accreditation. The majority of tests in Biochemistry, Endocrinology, Haematology,
Immunology, Microbiology, Histology and Anatomical Pathology are included. In
contrast to other quality standards such as ISO 9001:2008 and GLP (Good Laboratory
Practice), BS EN ISO/IEC 17025 is the only internationally recognised standard for
testing laboratories that specifically demonstrates technical competence and the
ability to generate technically valid results.
Reference ranges provided with results for guidance are based on studies carried
out in the laboratory wherever possible, but also take into account published
information (which can reflect different methodologies and study populations). These
are constantly under review by the Clinical Pathology team using experience from
reporting cases and updated information from the veterinary field, and in our opinion
offer the best aid in interpretation of reported results. As with all reference ranges
some normal animals will lie outside these ranges.
The Quality Management System also requires that the business is managed in
such a way as to ensure customer requirements are foremost. It is crucial that we
understand our clients’ expectations – we do this via customer surveys, courtesy
calls and taking on board all comments received. If you would like to lodge a
complaint or let us know how we could improve our service then please contact the
customer support teams (contact details on back page). We aim to resolve problems
and, where appropriate, implement improved procedures as soon as possible.
We will inform you of the outcome, including follow up contact, to ensure ongoing
satisfaction. As a result, we put resources into those areas of our service that you
consider to be of primary importance.
Anne-Marie Porritt
IDEXX Laboratories Ltd
Tel: 00800 1234 3399 · Fax: 01937 544001
labhelp@idexx.com · www.idexx.co.uk
HND Quality Manager
15
Postal Addresses
IDEXX Laboratories
Wetherby
IDEXX Laboratories
London
IDEXX Laboratories
Lea Green
Grange House
Sandbeck Way, Wetherby
West Yorkshire, LS22 7DN
84 New Wanstead
London, E11 2SY
Unit 12 Lea Green Business Park
Lea Green Ind Estate
Eurolink
St Helens, WA9 4TR
Sample Delivery
Wetherby
Point-of-care
Analysers
IDEXX Laboratories, PO Box 4
Wetherby, West Yorkshire, LS22 4YY
IDEXX Laboratories Ltd
Riverside House
Riverside Walk, Windsor
Berkshire SL4 1NA
www.idexx.co.uk
16
© 2015 IDEXX Laboratories, Inc. All rights reserved. • 14100039-1014-UK (UK214)