Guidelines for Equipment Usage
Transcription
Guidelines for Equipment Usage
Guidelines for HIR Central Facility: AKTA Purifier Plus 10 The AKTA Purifier Plus 10 versatile, modular liquid chromatography systems for fast and reliable separations of proteins and peptides. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The AKTA Purifier Plus 10 is at present located at the HIR building, central facility 2. Operating Hours The AKTA Purifier Plus 10 is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data If necessary, data can be saved using CD (supplied by the user) only. Charges The AKTA Purifier Plus 10 is FOC for UM-researcher and RM20 per run for non-UM researchers if researcher bring their own columns. Columns are available for rent : No 1 2 3 4 5 6 7 8 7 6 7 Columns MONO Q 4.6/100 PE MONO S 4.6/100 PE Superdex 75 10/300 GL Hitrap Desalting 5ml HiTrap IEX selection kit 1ml HiTrap Protein A HP, 1ml HiTrap Protein G HP 1ml HiTrap Heparin 1ml HiTrap SP HP 5ml HiTrap Q HP 5ml Superdex 200 10/300 GL Cat. # 17-5179-01 17-5180-01 17-5174-01 17-1408-01 17-6002-33 17-0402-03 17-0404-03 17-0406-01 17-1152-01 17-1154-01 17-5175-01 UM (per run) 25 25 25 5 5 10 10 5 5 5 25 Non-UM (per run) 100 100 100 20 20 40 40 20 20 20 50 If column spoiled due to mishandling by the user, user is responsible for the cost of replacing the column. Discounted package is available for frequent user. RM1500 per year for unlimited access. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All buffer including 20% EtOH will not be provided. All charges are not inclusive of general reagents or consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms (03-7967 7075, melim@um.edu.my), Dr. Ea Chee Kwee (eacheekwee@um.edu.my) Lim Moo Eng Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Atomic Emission Spectrometry (AES) The HIR Agilent 4100 MP-AES runs unattended without flammable or expensive gas supply. This unit runs entirely on air, which means one can perform automated multi-element analyses while eliminating flammable and expensive gases. This unit offers: Powerful performance - The magnetically excited microwave plasma source provides detection limits down to sub ppb levels. Better reliability - The robust system is built for a range of industries including Toxicology studies, Geochemistry, Food Testing and Agriculture, Energy and Fuels, and Environmental, and is ideal for remote environments. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR AES is at present located at the HIR building Fume Hood Room at 1st Floor. Operating Hours The HIR AES is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least one working day before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges For half-day usage, it will be free-of-charge for UM researchers and RM400 for non-UM researchers. For one-day usage, it will be RM50 for UM researchers, and RM800 for non UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Responsible officers are Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) and Dr Ea Chee Kwee (eacheekwee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Bioanalyzer Expert 2100 The HIR Bioanalyzer Expert 2100 is a multi-user facility which provides fast and user friendly instrumentation to researchers for analyzing nucleotide and protein’s concentration and purity. All consumable (Reagent and Chip) shall be provide by user. Before and after usage of the Bioanalyzer, user are responsible to clean the detector head with clean ultrapure water with the particular chip. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Bioanalyzer is located at Central Facility 2, Level 1, HIR Building Operating Hours The HIR Bioanalyzer is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at 30 min max for each sample including priming the chip. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each sample is chargeable at RM10 per sample for UM researchers and RM50 per sample for non-UM researchers. For frequent users, 50 sample is available at a discounted rate of RM400 per package. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR BIOLOG Facility The HIR BIOLOG Facility is a multi-user facility which provides advanced, comprehensive automated high throughput phenotype microarray system for quantitative phenotypic cellular assays with microbial cells and mammalian cells; integrated for automatic characterization and identification technology of bacteria [aerobic and anaerobic], yeasts and filamentous fungi. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR BIOLOG Facility is at present located at the 1st Floor Centre HIR lab, HIR Building. Operating Hours The HIR BIOLOG Facility is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. Strictly no block booking is allowed. Slots for usage are set at three-hours each i.e. 10 am – 1 pm and 2 pm – 5 pm, or from 10 am - 5pm. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer-in-charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges It is chargeable at RM100.00 per day slot for UM researchers and RM400.00 per day slot for nonUM researchers. For frequent users, a 10 slot-package (one day slot) is available at a discounted rate of RM800 per package. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents, buffers and consumables, BIOLOG plates etc. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr. Chan Kok Gan (kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Biomark HD and C1 Single-Cell auto Prep System The HIR Biomark HD and C1 Single-Cell auto Prep System facility is a multi-user facility which provides resarchers with cutting edge Biomark HD and C1 Single-Cell auto Prep System facility for latest research in single-cell study. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Biomark HD and C1 Single-Cell auto Prep System facility is at present located at Central Facility 2.2, Level 2, HIR Building. Operating Hours The HIR Biomark HD and C1 Single-Cell auto Prep System facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges For three-hour (and/or shorter time) is RM100 and for 1 day (nine-to-five working hour) slot is available at RM200 per day for UM researchers. Each three-hour slot is chargeable RM200 and RM400, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM600 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Lim Phaik Em (03-79677180, phaikeem@um.edu.my) and Dr. Tee Kok Keng (03-79677833, k2tee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Bio-Rad CFX96 Real Time PCR Facility The Bio-Rad CFX96 Real Time Facility is a multi-user facility which provides instrumentation to researchers for qPCR etc. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Bio-Rad CFX96 Real Time Facility is located at Central Facility 2, Level 1, HIR building. Operating Hours The Bio-Rad CFX96 Real Time Facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each one hour is chargeable at RM10 for UM researchers and RM50 for non-UM researchers. For frequent users, a 100 hour-package is avalaible at a discounted rate of RM950 and a 200 hour-package is avalaible at a discounted rate of RM1800 and a 500-hour-package is available at RM3000. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr. Ng Ching Ching (03-79675872) or Dr Ea Chee Kwee (eacheekwee@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Capillary Electrophoresis The HIR Capillary electrophoresis (CE) is a high efficiency and resolution, rapid analysis time, plus minimal sample and solvent requirements. It is an established technique in many laboratories. User are responsible on bringing their own solvents, column, and sample vial for each run. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Capillary Electrophoresis is at present located at the Bilik Khas 3, Ground Floor of HIR building. Operating Hours The HIR Capillary Electrophoresis is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at maximum 3 hours for each usage. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each use is chargeable at RM50 per sample for UM researchers and RM100 per sample for non-UM researchers. For frequent users, 50 slot is available at a discounted rate of RM1500 per package. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Agilent Cary 60 UV-VIS Spectrophotometer The HIR Cary 60 UV-VIS facility is a multi-user facility which can handle the widest variety of sample sizes and types with liquid sampling accessories including fiber optic probes and couplers, Peltier and water thermostatted single and multicell holders, temperature probes, microvolume sampling cells and rapid mix accessory for stopped flow kinetics. For solid samples, choose from solid sample holder, fiber optic reflectance probe and coupler and fixed angle specular reflectance accessories (SRA). Location The HIR Cary 60 UV-VIS facility is at present located at Central Facility 1, Level 1, HIR Building. Operating Hours The HIR Cary 60 UV-VIS facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only (if acpplicable). Charges Both three-hour (and/or shorter time) and nine-to-five working hour slots are available gratis (free-of-charge) for UM researchers. Each three-hour slot is chargeable RM100 and RM200 for nine-to-five hour slots, respectively, for non-UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Bio-Rad Chemidoc MP The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to address multiplex fluorescent western blotting, chemiluminescence detection, and general gel documentation applications. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The ChemiDoc MP system is located at Demo Teaching Lab, Ground Floor, HIR building. Operating Hours The ChemiDoc MP system is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD or authorized USB-thumb drive only. Charges Each one hour is chargeable at RM10 for UM researchers and RM50 for non-UM researchers. For frequent users, a 100 hour-package is avalaible at a discounted rate of RM950 and a 200 hour-package is avalaible at a discounted rate of RM1800 and a 500-hour-package is available at RM3000. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my) or Dr. Lau Yee Ling (03-79674749) lauyeeling@um.edu.my Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Chromatin Immunoprecipitation (ChiP) Automated System which includes (a) Bioruptor & (b) SX-8G IP-Star automated System The HIR Chromatin Immunoprecipitation (CHIP) Automated System which includes (a) Bioruptor & (b) SX-8G IPStar automated System facility is a multi-user facility which provides resarchers with cutting edge on ChiPSeq preparation facility for latest research. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Chromatin Immunoprecipitation (CHIP) Automated System facility is at present located at Ground Floor, HIR Building. Operating Hours The HIR Chromatin Immunoprecipitation (CHIP) Automated System facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges FOC for Bioruptor. For SX-8G IP-STAR system, three-hour (and/or shorter time) is RM100 and for 1 day slot is available at RM200 per day for UM researchers. Each three-hour slot is chargeable RM200 and RM400, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM600 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Lim Phaik Em (03-79677180, phaikeem@um.edu.my) Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR BioRad QX200 Droplet Digital PCR System The HIR Droplet Digital PCR facility is a multi-user facility which provides resarchers with absolute quantification of target DNA molecules using probe-based digital PCR applications.. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Droplet Digital PCR facility is at present located at Ground Floor, HIR Building. Operating Hours The HIR Droplet Digital PCR facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges RM20 per run for UM researchers. RM50 per run for non-UM researchers. For frequent users, 100 runs-package is available at a discounted rate of RM1000 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Ng Ching Ching (03-79675628, ccng@um.edu.my) and Dr. Ea Chee Kwee (03-79677022-2587, eacheekwee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. 1st Version Guidelines for using the Chemostat (Fermenter) The HIR Chemostat (fermenter) facility is a multi-user facility which provides resarchers with chemostat (fermenter) for continuos fermenation or cell culturing. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Chemostat (fermenter) facility is at present located at Ground Floor, HIR Building. Operating Hours The HIR Chemostat (fermenter) facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. But due to the nature of experiment involving chemostat (fermenter) facility that can span >3 days, the user must state clearly that how long the experiments will require to complete, which will be approved by HIR committee on case-by-case basis. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only (if acpplicable). Charges Both three-hour (and/or shorter time) and nine-to-five working hour slots are available gratis (freeof-charge) for UM researchers. Each three-hour slot is chargeable RM100 and RM200, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM100 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Fluorescence Microscope The HIR Fluorescence Microscope facility is a multi-user facility which provides resarchers with Fluorescence Microscope for fluoresence and related microscopy work. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Fluorescence Microscope facility is at present located at 1st Floor, HIR Building. Operating Hours The HIR Fluorescence Microscope facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Both three-hour (and/or shorter time) and nine-to-five working hour slots are available gratis (freeof-charge) for UM researchers. Each three-hour slot is chargeable RM100 and RM200, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM100 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Agilent Gas Chromatography-Sherlock MIDI Microbial Identification System The Agilent Gas Chromatography-MIDI Microbial Identification System is a multi-user facility that offers researchers with identification of bacteria based on analysis of cellular lipidic fraction of bacteria such as Fatty Acid Methyl Esters (FAME). Determination of the FAMEs was coupled with the use of Agilent Gas Chromatography hence it allows rapid bacterial identification without the requirement of biochemical kits. All consumables and sample preparations have to be provided by user. Users are responsible to make sure the machine is not contaminated with their sample (especially bacteria extracts) after usage. User are adviced to switch on the instrument 30 mins before use. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR GC-MIDI is at present located at the HIR building Bilik Khas 3. Operating Hours The HIR GC-MIDI is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at maximum 50 samples per usage. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each sample is chargeable at RM15 per sample for UM researchers and RM50 per sample for non-UM researchers. For frequent users, 50 sample is available at a discounted rate of RM700 per package. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Revised April 2015 Guidelines for Using the HIR Agilent 5975C GC/MSD Facility Gas chromatography-mass spectrometry (GC-MS) is an instrument that separates chemical compounds in a mixture (GC component) and identifies the separated compounds by their mass over charge ratio (MS component). GC-MS is used in a wide range of applications including analysis of lipids and drug metabolites, environmental analysis and identification of chemical compounds in a mixture. One of the advantages of GC-MS is its ablity to provide identification of chemical compounds based on the mass spectra provided in the incorporated library. The Committee reserves the right to review and make any necessary changes to the Guidelines as and when needed. Brand and Model Agilent 7890A GC System equipped with 5975C inert MSD Mass Spectrometer Location: Bilik Khas 3, Level G, HIR Building Operating Hours The Agilent 5975C GC/MSD facility is available for service from 10.00am to 5.00pm, working weekdays. (Special arrangement may be made for overnight or over weekend analysis, if necessary.) Advanced appointment (minimum 2 weeks in advance) is required. Sample Preparation Requirements The samples MUST BE ABLE TO VOLATILISE at temperatures below 300C. Thus, samples of total plant extracts, untreated waste-water, crude oil, strong acid or base solutions, salts, metals and polymeric materials are not suitable to be analysed by GC-MS. Samples must be prepared in guranteed grade (GR) or GC-MS grade solvents, preferably acetone, methanol or hexane. Less preferable are halogenated solvents (e.g., chloroform, methylene chloride, etc.) and ethers (e.g., diethylether, THF). DO NOT use water, DMF, or DMSO as solvent. The upper limit of sample concentration is 100.0 ppm in 0.5–1.5 mL. Overloading samples may cause damage to the ion source in the MS component. Every sample must be filtered through a 0.45 µm membrane filter before injection into the GC-MS. Every sample must be placed in a GC-MS specified sample vial with the correct septum and cap, i.e., Agilent Part Number 5182-0714 (Pack of 100 screw top vials) and Agilent Part Number 5182-0717 (Pack of 100 blue screw caps and PTFE/red rubber septas). Software Package NIST 2011 Library, Deconvolution and Reporting software, Fiehn GC/MS Metabolomics RTL Library, MassHunter Data Analysis for GCMS Online Booking Only qualified users will have access to the booking calendar. You must book online to use the equipment. The slots are scheduled on a first-book first-serve basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointment and show up on time. Every user must complete the experiments within the time slot allocated. Otherwise, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. Otherwise, for late cancellation, a user will be charged 50% of the cost of the booked session. Users will have to fill up a letter of intent available online. For all projects please consult with the Officers-in-charge prior to sample submission. We look forward to working with users and meeting users’ expectations. Export of Data: Data will be saved using CD/ DVD only. 1 2 3 Charges The Committee reserves the right to revise the charges as and when needed. UM Users Non UM Users Frequent UM Users Sample RM50.00/sample RM265.00/sample RM474.00/10 samples (Injection) or analysis1,2,3 (Injection) (Injection) RM2,250.00/50 samples (Injection) Excludes sample preparation and data analysis Every user to provide an appropriate GC column other than the standard column, HP-5ms [J&W GC Column - (5% Phenyl) methylpolysiloxane - Cat No. 19091S-433] GC-MS specified sample vial to be provided by every user Contact persons For booking and to purchase a rental package, please contact the officer-in-charge, Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my). For technical advice, please contact Dr Lee Guan Serm (03-7967 2577, leeguanserm@um.edu.my) or Dr. Loke Mun Fai (lokemunfai@um.edu.my) Prerequisites User must have received training on this equipment or demonstrate knowledge of the equipment before use. User has to run the equipment according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Agilent GC-QTOF Mass Spectrometer The HIR Agilent GC-QTOF Facility is a multi-user facility which provides advanced instrumentation to researchers for volatile compound mass spectrometry analysis. User must provide reference of journals, publication or application notes to confirm that the samples must be amenable to GC techniques and reference to GC-QTOF methods such as suitable mobile phase, gradient composition, types of GC columns and MS detector settings are available. Samples must go through proper sample preparation or clean-up procedure prior to GC injection. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Agilent GC-QTOF Facility is at present located at HIR Building Ground Floor, Bilik Khas 3. Operating Hours The HIR Agilent GC-QTOF Facility is available for service from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental. Cancellation of booking must be made at least one working day before the actual equipment operation time. Users will be billed for the full charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges Each three-hour slot is chargeable at RM100 per slot for UM researchers and RM1800 per slot for non-UM researchers. For nine-to-five working hour, it is chargeable at RM200 per slot for UM researchers and RM3500 per slot for non-UM researchers. For frequent users, 100 hour-package is available at a discounted rate of RM2000 per package and 200 hourpackage is avalaible at a discounted rate of RM3000. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Responsible officers are Dr Loke Mun Fai (lokemunfai@um.edu.my), Dr. Chan Kok Gan (kokgan@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Ion Torrent Personal Genome NGS The HIR Ion Torrent Personal Genome NGS facility is a multi-user facility which provides resarchers with cutting edge NGS platform. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Ion Torrent Personal Genome NGS facility is at present located at 1st Floor, HIR Building. Operating Hours The HIR Ion Torrent Personal Genome NGS facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges For 1 day (nine-to-five working hour slot) is available at RM200 per day for UM researchers and RM1,000 per day for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM1,000 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Lim Phaik Em (03-79677180, phaikeem@um.edu.my) and Dr. Chan Kok Gan (0379674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Isothermal Titration Calorimeter (ITC) The HIR ITC allow characterization of biomolecular interactions by providing direct, label-free measurements of binding affinity and thermodynamics. ITC provides valuable information about binding interactions, molecular stability, protein folding, and enzyme kinetics. ITC measures all binding parameters in a single label-free, in-solution experiment. Parameters include binding affinity (KD), reaction stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). ITC is useful for characterizing biomolecular interactions and the study of the mechanism of the molecular interaction. Key applications include: Quantitation of binding affinity Measurement of thermodynamics and active concentration Characterization of mechanism of action Confirmation of intended binding targets in small molecule drug discovery Determination of binding specificity and stoichiometry Validation of IC50 values during hit-to-lead Measurement of enzyme kinetics The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR ITC is at present located at the HIR building, 1st Floor, Central Lab. Operating Hours The HIR ITC is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least one working day before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges For half-day usage, it will be free-of-charge for UM researchers and RM800 for non-UM researchers. For one-day usage, it will be RM50 for UM researchers, and RM1500 for non UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Responsible officers are Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) and Dr Ea Chee Kwee (eacheekwee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Agilent 6490 LC/MS/MS Triple Quadrupole Mass Spectrometer The HIR Agilent 6490 LC/MS/MS Facility is a multi-user facility which provides advanced instrumentation to researchers for most sensitive quantification tool of targeted compounds with reduce sample preparation. User must provide reference of journals, publication or application notes to confirm that the samples must be amenable to LC techniques and reference to LC/MS/MS methods such as suitable mobile phase, gradient composition, types of LC columns and MS detector settings are available. Samples must go through proper sample preparation or clean-up procedure prior to LC injection. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Agilent 6490 LC/MS/MS Facility is at present located at HIR Building Ground Floor, Bilik Khas 3. Operating Hours The HIR Agilent 6490 LC/MS/MS Facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to 3 days in advance. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each three-hour slot is chargeable at RM100 per slot for UM researchers and RM1500 per slot for non-UM researchers. For nine-to-five working hour, it is chargeable at RM200 per slot for UM researchers and RM3000 per slot for non-UM researchers. For frequent users, 100 hour-package is available at a discounted rate of RM2000 per package and 200 hour-package is avalaible at a discounted rate of RM3000. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Responsible officers are Dr Loke Mun Fai (lokemunfai@um.edu.my), Dr. Chan Kok Gan (kokgan@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for HIR Central Facility: Luminex 200 The Luminex 200 is a multi-user facility which provides advanced instrumentation of multiplex bead-based fluoroanalyzer based on the xMAP technology. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Luminex 200 is at present located at Central Facility 1, Level 1, HIR building. Operating Hours The Luminex 200 is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at 5 hours per run of a 96-well plate (including time for calibration, warm-up, detection and analysis, maintenance and shutdown). The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data If necessary, data can be saved using CD (supplied by the user) only. Charges Each three-hour slot is chargeable at RM200 per slot for UM researchers and RM500 per slot for non-UM researchers. For frequent users, a 10-slot package is available at a discounted rate of RM1,200 per package. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents or consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr. Tee Kok-Keng (k2tee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR MacsQuant Flow Cytometry Facility The HIR MacsQuant Flow Cytometry Facility is a multi-user facility which provides advanced instrumentation to researchers for analyzing various types of cells according to their measurable properties. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR MacsQuant Flow Cytometry Facility is at present located at the Central Facility Lab 2, Level 1, HIR building. Operating Hours The HIR MacsQuant Flow Cytometry Facility is available for service 24 hrs/ 7 days. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using Thumb drive only. Charges Each hour is chargeable at RM50.00 per slot for UM researchers and RM200.00 per hour for non-UM researchers. For frequent users, a 100 hours-package is available at a discounted rate of RM4,000.00 per package and a 300 hours-package is available at a discounted rate of RM8,000. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents, buffers and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr. Ea Chee Kwee (eacheekwee@um.edu.my), Dr. Ng Ching Ching (ccng@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability Guidelines for using the Bruker MALDI-TOF Mass Spectrometer The HIR Bruker MALDI TOF Mass Spectrometer facility is a facility which provides advanced instrumentation to researchers working on biomarker discovery, oligonucleotides, small molecules, polymer analysis and short peptide analysis. This instrument is useful for clinical proteomics, functional genomics, small molecules, and microbiology. Samples must go through proper sample preparation or clean-up procedure prior to analysis. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR MALDI TOF Mass Spectrometer facility is at present located at HIR Building 1st floor, Central Lab. Operating Hours The HIR MALDI TOF Mass Spectrometer facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to 3 days in advance. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each three-hour slot is chargeable at RM100 per slot for UM researchers and RM1500 per slot for non-UM researchers. For nine-to-five working hour, it is chargeable at RM200 per slot for UM researchers and RM3000 per slot for non-UM researchers. For frequent users, 100 hour-package is available at a discounted rate of RM2000 per package and 200 hour-package is avalaible at a discounted rate of RM3000. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Responsible officers are Dr Loke Mun Fai (lokemunfai@um.edu.my), Dr. Chan Kok Gan (kokgan@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Microarray System The HIR Affymetrix microarray facility provides resarchers with cutting edge microarray for genes expression profile work and related work. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR micromanipulator facility is at present located at 1st Floor, HIR Building. Operating Hours The HIR microarray facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges For one day usage (eight-to-fve, and/or shorter time) is RM150 and 2 days slot is available at RM250 for UM researchers; and RM500 and RM800, respectively, per slot, for non-UM researchers. For frequent users, 10-day package is available at a discounted rate of RM800 per package. This package is available only for UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Ea Chee Kwee (03-79675889, eacheekwee@um.edu.my) Dr. Chan Kok Gan (0379674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Micromanipulator The HIR micromanipulator facility is a multi-user facility which provides resarchers with cutting edge micromanipulator for latest micromanipulator related work. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR micromanipulator facility is at present located at 1st Floor, HIR Building. Operating Hours The HIR micromanipulator facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges For three-hour (and/or shorter time) is RM50 and for nine-to-five working hour slots are available at RM150 per day for UM researchers. Each three-hour slot is chargeable RM100 and RM300, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM300 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Lim Phaik Em (03-79677180, phaikeem@um.edu.my) and Dr. Tee Kok Keng (0379677833, k2tee@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Revised Rate 27 Dec 2012 Guidelines for using the HIR Microplate Reader (M200 Pro) The HIR Microplate Reader (M200 Pro) is a multi-user facility which provides advance instrumentation to researchers to read the absorbance, luminescence and fluorescence at different time frame. The measurement of absorbance, luminescence and fluorescence is fully automated by the machine and hence it is a very easy to use machine. All consumable (96 or 384 well plate) shall be provide by user. User are responsible to make sure the machine is not contaminated with their sample (especially bacteria sample) after usage. User are advice to preset the temperature of the machine 15min before use. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Microplate Reader (M200 Pro) is at present located at the Central Facility 2, Level 1, HIR Building. Operating Hours The HIR Microplate Reader (M200 Pro) is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at maximum 3 days for each usage. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Unit of Measurement Per plate of 96 wells, Quick Read Overnight Read UM User FOC 50.00 Charges (RM) Non UM User Frequent UM User 100.00 Nil 1,500.00/50 plates The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Revised Rate 27 Dec 2012 Guidelines for using the HIR Microplate Reader (F200 Pro) The HIR Microplate Reader (F200 Pro) is a multi-user facility which provides advance instrumentation to researchers to read the absorbance, luminescence and fluorescence at different time frame. The measurement of absorbance, luminescence and fluorescence is fully automated by the machine and hence it is a very easy to use machine. All consumable (96 or 384 well plate) shall be provide by user. User are responsible to make sure the machine is not contaminated with their sample (especially bacteria sample) after usage. User are advice to preset the temperature of the machine 15min before use. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Microplate Reader (F200 Pro) is located at Central Facility 2, Level 1, HIR Building. Operating Hours The HIR Microplate reader (F200 Pro) is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at maximum 3 days for each usage. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Unit of Measurement Per plate of 96 wells, Quick Read Overnight Read UM User FOC 50.00 Charges (RM) Non UM User Frequent UM User 100.00 Nil 1,500.00/50 plates The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Microscopes (High Resolution Compound Microscope, Digital Motorized Inverted Microscope with Fluorescence and Digital Imaging System and Stereo microscope) The HIR Microscopes facility is a multi-user facility which provides resarchers with various types of microscopes for latest microscopy work. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Microscopes facility is at present located Microscopy Room, Level 2, HIR Building. Operating Hours The HIR Microscopes facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walkin usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges Rental for usage of either (i) Up-right Fluorescence, (ii) Inverted Fluorescence or (iii) Stereo microscope: Half-day slot (either from 9.00 am – 1.00 pm or from 1.00 – 5.00 pm) is RM10.00 For frequent users: 50 hour package is available at a discounted rate of RM100.00, 100-hour package at RM180.00 and 500-hour package at RM750.00. These discounted packages are available only to UM users with priority to UM HIR grant holders. For non-UM users, both three-hour and nine-to-five working hour slots are chargeable at RM100 and RM200, respectively. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Lim Phaik Em (03-79677180, phaikeem@um.edu.my) and Dr. Ea Chee Kwee (0379675889, eacheekwee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Nanostring (GEN2) Facility The HIR Nanostring (GEN2) Facility which is based on a digital molecular barcoding technology is a multi-user facility that can deliver highly multiplexed, direct digital profiling of individual molecules in a single reaction without amplification or bias. It application includes profiling hundreds of gene transcripts, copy number variations, or miRNAs with high sensitivity and precision. The NanoString nCounter gene expression system was reported to be more sensitive than microarrays but similar to real-time PCR (Taqman and SYBRGreen). Location The HIR Nanostring (GEN2) Facility is at present located at the 1st Floor Centre HIR lab, HIR Building. Operating Hours The HIR Nanostring (GEN2) Facility is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. Strictly no block booking is allowed. The PrepStation can only work on one single catridge (~3hr) at a single time while the Digital analyzer can scan maximum 6 catridge at any time (~6hr). Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer-in-charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using the original USB thumbdrive that come along with the kit (containing the RLF file). Charges Currently, there will be no charge to encourage users to try and use the equipment. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Dr Ea Chee Kwee (03-79675889, eacheekwee@um.edu.my), Dr. Lim Yat Yuen (yatyuen.lim@um.edu.my) Prerequisites The system includes a fully automated sample Prep Station, a Digital Analyzer. Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. In order to run assays using the Nanostring system, CodeSet (molecular barcodes) and all reagents needed to perform the analysis are needed and to be bought individually. Please bring the USB stick that comes along with the assay kit to upload the RLF file onto the Digital counter (incase the RLF file have not been uploaded before). The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed Guidelines for using the Bio-Rad Next Generation Chromatography (NGC) Facility The Bio-Rad Next Generation Chromatography (NGC) Facility is a multi-user facility which provides instrumentation to researchers for chromatography / protein purification. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Bio-Rad Next Generation Chromatography (NGC) Facility is located at Central Facility 2.1, Level 2, HIR building. Operating Hours The Bio-Rad Next Generation Chromatography (NGC) Facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD or authorized USB-thumb drive only. Charges Each one hour is chargeable at RM30 for UM researchers and RM100 for non-UM researchers. For frequent users, a 100 hour-package is avalaible at a discounted rate of RM2500 and a 200 hour-package is avalaible at a discounted rate of RM4500 and a 500-hour-package is available at RM10,000. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my), or Dr Lau Yee Ling (03-79674749) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. 2nd Version Guidelines for HIR Central Facility: NucliSENS easyMAG The NucliSENS easyMAG is a multi-user facility which provides advanced instrumentation for total nucleic acid purification. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The NucliSENS easyMAG is at present located at Central Facility 2, Level 1, HIR building. Operating Hours The NucliSENS easyMAG is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at 2 hours per run of 24 samples. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data If necessary, data can be saved using CD (supplied by the user) only. Charges The NucliSENS easyMAG operates effectively in the multiple of eight (8) samples. The charge for every 8 samples is RM320 for UM researchers and RM800 for non-UM researchers. For users with more than 48 samples, a 5% discount will be given. This discounted package is available only for UM researchers with priority for UM HIR grant holders. Lysis Buffer (#280134) and Magnetic Silica (#280133) will not be provided. All charges are not inclusive of general reagents or consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Tee Kok-Keng (k2tee@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Orbitrap Fusion Mass Spectrometer The HIR Orbitrap Fusion Mass Spectrometer which includes (a) UHPLC (b) NanoLC System facility is a multi-user facility which provides resarchers with cutting edge on Orbitrap mass spectrometry analysis. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Orbitrap Fusion Mass Spectrometer facility is at present located at Ground Floor, HIR Building. Operating Hours The HIR Orbitrap Fusion Mass Spectrometer facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges For three-hour (and/or shorter time) is levied at RM200. For 1 day (nine-to-five working hour) slot and 2day slot, it is available at RM300 and RM400, respectively, for UM researchers. Each three-hour slot is chargeable RM400. For 1 day (nine-to-five working hour) slot and 2-day slot, it is available at RM1,000 and RM1,500, respectively, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM1,500 per package. This package is available only for UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Loke Mun Fai (lokemunfai@um.edu.my) and Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Hamamatsu Photon Camera The HIR Hamamatsu Photon Camera facility is a multi-user facility which provides advanced instrumentation to researchers for most sensitive bioimaging with ultra senstive CCD camera. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Hamamatsu Photon Camera facility is at present located at Ground Floor, HIR Building. Operating Hours The HIR Hamamatsu Photon Camera facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each three-hour (and/or shorter time) is available gratis (free-of-charge) for UM researchers. Each three-hour slot is chargeable RM150 per slot for non-UM researchers. For nine-to-five working hour, it is chargeable at RM50 per slot for UM researchers and RM300 per slot for non-UM researchers. For frequent users, 100 hour-package is available at a discounted rate of RM200 per package. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Bio-Rad PROTEAN i12 IEF system The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This feature makes it possible to run different sample types, different pH gradients, and multiple protocols simultaneously. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The PROTEAN i12 IEF system is located at Central Facility 2.1, Level 2, HIR building. Operating Hours The PROTEAN i12 IEF system is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD or authorized USB-thumb drive only. Charges Each day is chargeable at RM15 for UM researchers and RM30 for non-UM researchers. For frequent users, a one-month-package is avalaible at a discounted rate of RM270. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my) or Dr. Lau Yee Ling (03-79674749, lauyeeling@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and li Guidelines for using the Bio-Rad ProteOn XPR36 system Facility The Bio-Rad ProteOn XPR36 system Facility is a multi-user facility which provides instrumentation to researchers for protein-interaction studies. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Bio-Rad ProteOn XPR36 system Facility is located at Central Facility 2.1, Level 2, HIR building. Operating Hours The Bio-Rad ProteOn XPR36 system Facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific prearrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD or authorized USB-thumb drive only. Charges UM user: RM 50/day RM1000 for maintenance chip RM1000 for cleaning chip Non-UM user: RM 200/day RM3000 for maintenance chip RM2000 for cleaning chip Note: -Maintenance chip will be applicable for long-term shutdown in HIR’s setting (more than a week of without usage). So once a user has done the experiment and will not come back to use the instrument for more than 1 week, the maintenance chip should be inserted into the system. -Cleaning chip will be used by end user upon completion of their experiment with the same protein. This is for cleaning of the microfluidics channel to prevent risk of cross-contamination. So, each time a user finishes their experiment, they will use 1 cleaning chip. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my) or Dr. Lau Yee Ling (03-79674749) lauyeeling@um.edu.my Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. 2nd Version Guidelines for using the HIR Pulse Field Gel Electrophoresis (PFGE) The HIR PFGE resolves DNA by alternating electrical field between distinc pairs of electrodes. PFGE is good to resolve large DNA fragment of >10Mb, and chracterisation of genome size. HIR discourage staining of PFGE gels with ethidium bromide which is carcinogenic. HIR also provide high resolution gel imaging system for image capture of PFGE. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR PFGE is at present located at the HIR building,1st Floor, Central Lab. Operating Hours The HIR PFGE is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least one working day before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges For half-day usage, it will be free-of-charge for UM researchers and RM400 for non-UM researchers. For one-day usage, it will be free-of-charge for UM researchers, and RM800 for non UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Responsible officers are Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) and Dr Ea Chee Kwee (eacheekwee@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the ABI Quantstudio 12K Real Time PCR Facility The HIR ABI Quantstudio 12K Real Time Facility is a multi-user facility which provides advanced instrumentation to researchers for high throughput qPCR etc. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR ABI Real Time Facility is at present located at Central Facility 2, Level 1, HIR building. Operating Hours The HIR ABI Real Time Facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each one hour is chargeable at RM30 for UM researchers and RM100 for non-UM researchers. For frequent users, a 100 hour-package is available at a discounted rate of RM2500 per package, a 200 hour-package is avalaible at a discounted rate of RM4000 and a 500-hour package is available at RM9000. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr. Ng Ching Ching (03-79675872). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Revised Rate 27Mar 2013 Guidelines for HIR Central Facility: Qubit 2.0 Fluorometer The Qubit 2.0 Fluorometer is a multi-user facility which provides advanced instrumentation for fluorometric quantitation of biomolecules such as DNA, RNA and protein. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Qubit 2.0 Fluorometer is at present located at the Central Facility 2, Level 1, HIR Building. Operating Hours The Qubit 2.0 Fluorometer is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at 10 minutes (DNA, RNA) or 25 minutes (protein) per sample. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data If necessary, data can be saved using CD (supplied by the user) only. Charges Each sample will be FOC for UM researchers and RM25 for non-UM researchers. All charges are not inclusive of reagents or consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Tee Kok-Keng (k2tee@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Eyela Rotary Evaporator Facility The HIR Eyela Rotary Evaporator Facility is an apparatus developed as advanced instrumentation to researchers for rapid removal of excess solvent under reduced pressure in the microscale laboratory. Location The HIR Eyela Rotary Evaporator Facility is at present located at the Fume Hood Room, Level 1, HIR Building. Operating Hours The HIR Eyela Rotary Evaporator Facility is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time.You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data None Charges Each one hour is chargeable at RM30 for UM researchers and RM100 for non-UM researchers. For frequent users, 100 hour-package is available at a discounted rate of RM2500 per package and 200 hour-package is avalaible at a discounted rate of RM4000. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79675162, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Bio-Rad SDS Gel Electrophoresis System The SDS PAGE Gel Electrophoresis system is designed for proteins separating using SDS PAGE. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The SDS PAGE Gel Electrophoresis system is located at Central Facility 2.1, Level 2, HIR building. Operating Hours The SDS PAGE Gel Electrophoresis system is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD or authorized USB-thumb drive only. Charges Each day is chargeable at RM10 for UM researchers and RM15 for non-UM researchers. For frequent users, a one-month-package is avalaible at a discounted rate of RM200. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my) or Dr. Lau Yee Ling (03-79674749, lauyeeling@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letters of indemnity and liability Guidelines for using the HIR Tabletop Scanning Electron Microscopes (Tabletop SEM) The HIR Tabletop SEM facility is a multi-user facility which provides resarchers with cutting edge Tabletop SEM for latest electron microscopy work. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Microscopes facility is at present located at SEM Room, Level 2, HIR Building. Operating Hours The HIR Microscopes facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do the booking. No block booking is allowed. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking and all other procedures complied), also in such instances, the users who failed to turn up on time will still be liable to pay the full amount of rental (if applicable). Cancellation of booking must be made at least two working days before the actual equipment operation time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity available online. Export of Data Data has to be saved using CD only. Charges For three-hour (and/or shorter time) is RM100 and for nine-to-five working hour slots are available at RM200 per day for UM researchers. Each three-hour slot is chargeable RM200 and RM400, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM600 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr Lim Phaik Em (03-79677180, phaikeem@um.edu.my) Dr. Chan Kok Gan (0379674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Bio-Rad Mini Transblot Cell System The Transblot system is designed for Western blotting to transfer proteins from SDS PAGE to membrane. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Transblot system system is located at Central Facility 2.1, Level 2, HIR building. Operating Hours The Transblot system system is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD or authorized USB-thumb drive only. Charges Each day is chargeable at RM10 for UM researchers and RM15 for non-UM researchers. For frequent users, a one-month-package is avalaible at a discounted rate of RM200. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (0379677075, melim@um.edu.my) or Dr. Lau Yee Ling (03-79674749, lauyeeling@um.edu.my). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign letters of indemnity and liability. Guidelines for HIR Central Facility: Typhoon FLA 9500 Typhoon FLA 9500 is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Typhoon FLA 9500 is at present located at the HIR building central facility room2. Operating Hours The Typhoon FLA 9500 is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. Users will have to fill up a letter of intent available online. Export of Data If necessary, data can be saved using CD (supplied by the user) only. Charges The Typhoon FLA 9500 is FOC for UM-researcher and RM50 per scan for non-UM researchers. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Ea Chee Kwee (eacheekwee@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the HIR Agilent 1290 Ultra High Performace Liquid Chromatograph (UHPLC) The HIR Agilent 1290 UHPLC Facility is a multi-user facility which provides advanced instrumentation to researchers for qualitative and quantitative analysis of various types of polar and non-polar molecules of low or high molecular weight samples. User must provide reference of journals, publication or application notes to confirm that the samples must be amenable to LC techniques and reference to HPLC methods such as suitable mobile phase, gradient composition, types of LC columns and detectors are available. Samples must go through proper sample preparation or clean-up procedure prior to LC injection. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The HIR Agilent 1290 UHPLC Facility is located at Bilik Khas 3, Ground Floor, HIR building. Operating Hours The HIR Agilent 1290 UHPLC Facility is available for service from 10 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. Slots for usage are set at three-hours each i.e. 10 am – 1 pm and 2 pm – 5 pm. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each three-hour slot is chargeable at RM15 per sample for UM researchers and RM50 per slot for non-UM researchers. For frequent users, 50 slot-package is available at a discounted rate of RM600 per package and 100 slot-package is available at a discounted rate of RM 1000. This package is available only for UM researchers with priority for UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Beckman Coulter Optima XPN100 Ultracentrifuge Facility The Beckman Coulter Optima XPN100 Ultracentrifuge Facility is a multi-user facility which provides instrumentation to researchers for ultracentrifugation: purify and/or separation of viruses, subcellular organelles, macromolecules etc (SW 41 Ti, Type 70 Ti, and Type 100 Ti rotors are available, which rated for 41 000 rpm, 70 000 rpm, and 100 000 rpm, respectively). The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Beckman Coulter Optima XPN100 Ultracentrifuge Facility is at present located at Bilik Khas 2, HIR building. Operating Hours The Beckman Coulter Optima XPN100 Ultracentrifuge Facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Not applicable. Charges Each one hour is chargeable at RM10 for UM researchers and RM50 for non-UM researchers. For frequent users, a 100 hour-package is avalaible at a discounted rate of RM950 and a 200 hour-package is avalaible at a discounted rate of RM1800 and a 500-hour-package is available at RM3000. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr. Ng Ching Ching (03-79675872). Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the Vacuum Concentrator The HIR Vacuum Concentrator facility is a multi-user facility which provides resarchers with Vacuum Concentrator for rapid drying of biomolecules. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Users must ensure the solvent used is compatible to the Vacuum Concentrator. Location The HIR Vacuum Concentrator facility is at present located at Microbiom 3, Level 1, HIR Building. Operating Hours The HIR Vacuum Concentrator facility is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only (if applicable). Charges Both three-hour (and/or shorter time) and nine-to-five working hour slots are available gratis (freeof-charge) for UM researchers. Each three-hour slot is chargeable RM100 and RM200, respectively, per slot, for non-UM researchers. For frequent users, 10 days-package is available at a discounted rate of RM100 per package. This package is available only for UM researchers with priority to UM HIR grant holders. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075, melim@um.edu.my), Dr. Chan Kok Gan (03-79674509, kokgan@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability. Guidelines for using the VITEK® 2 System The Biomérieux VITEK® 2 system is a multi-user facility which provides instrumentation to researchers for microbial identification and antibiotic susceptibility testing. The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and when needed. Location The Biomérieux VITEK® 2 system is located at Central Facility 2, Level 1, HIR building. Operating Hours The Biomérieux VITEK® 2 system is available for service from 9 am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The number of people that can be present from each user laboratory is limited to two, except by specific pre-arrangement with the facility staff. Online Booking Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize availability for the entire UM community. Please keep your appointments, and show up for them promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time. You will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of intent available online. Export of Data Data has to be saved using CD only. Charges Each day (24 hours) is chargeable at RM100 for UM researchers and RM200 for non-UM researchers. For frequent users, a 10 day-package is avalaible at a discounted rate of RM800. This discounted package is available only for UM researchers with priority for UM HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any necessary adjustment to the charges when needed. Contact persons For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, melim@um.edu.my), Dr Chai Lay Ching (lcchai@um.edu.my) and Prof Ngeow Yun Fong (yunngeow@um.edu.my) Prerequisites Users must have received training on this machine or demonstrate knowledge of the equipment before use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability.