Laser Ablation Tomography Plant Biology 2015 Poster

Laser Ablation Tomography
New discoveries begin with LAT™
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Laser Ablation Tomography (LAT™) is capable of rapidly acquiring structural and compositional data in three dimensions with micron resolution. LAT™ uses fluorescent and
hyperspectral imaging to obtain highly contrasted, colorful stacks of images in opaque biological specimens.
An ultraviolet laser-sheet is created with a 355 nanometer q-switched, pulsed laser and a
rapidly-swept galvanometer scanner. A camera focused on the face of the laser-sheet cap-
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tures images of the sample as it is being incrementally pushed into the sheet from the opposite side. Due to the Gaussian distribution of the laser-sheet radiation, the surface layer
of the sample that is being pushed into the laser-sheet is auto-fluoresced just prior to ablation. This process is continuously repeated until the desired sample volume is achieved.
The high intensity of the laser-sheet induces fluorescent emission in the sample’s surface
layer, producing distinct spectral characteristics depending on molecular composition.
After imaging a complete sample, the stack of images is processed and reconstructed into
LAT™ Process - (1) Cross-sectional images produced of Maize root. (2) Successive images are stacked together. (3) A 3D rendering of the sample is created. (4 & 5) 3D rendering can be segmented to quantify features of interest. a high-resolution volume rendering that can be viewed, manipulated and virtually dissected. Depending on the sample material and its size, images can be acquired at a rate greater than ten per second. This method allows for data acquisition at unprecedented speed
without the need for staining or complex preparatory procedures.
LAT™ is ideally suited for life science applications such
as high-throughput phenotyping, general anatomical
inquiries, and scientific discovery.
Featuring:
• Geometric and Microanatomic Structure Visualization
• Compositional Visualization
• Morphometric Analysis
• Hyperspectral Imaging
• Fluorescent Microscopy
• Micron Resolution
• Tomographic Models & Animations
Lasers for Innovative Solutions llc
200 Innovation Blvd. Suite 214
State College, PA 16803
T: (814) 531-5447
E: brain.reinhardt@l4is.com
W: www.l4is.com