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Operating Manual
MIRAX DESK
Carl Zeiss
COPYRIGHT / WARENZEICHEN
MIRAX DESK
Knowledge of this manual is required for the operation of the instrument. Would you therefore please
make yourself familiar with the contents of this manual and pay special attention to hints concerning safe
operation of the instrument.
The specifications are subject to change; the manual is not covered by an update service.
©
Unless expressly authorized, forwarding and duplication of this document, as well as utilization and
communication of its contents are not permitted. Violations will entail an obligation to pay
compensation.
All rights reserved in the event of granting of patents or registration of a utility model.
All names of companies and products mentioned in this manual may be trademarks or registered
trademarks. The quoting of product names is for information purposes only and does not represent a
trademark misuse.
Carl Zeiss MicroImaging GmbH is not liable for the performance or use of these products.
MIRAX DESK was developed, produced and licensed exclusively for Zeiss by 3DHISTECH Kft./Hungary.
Issued by:
Carl Zeiss MicroImaging GmbH
P.O.B. 4041, 37030 Göttingen, Germany
Phone:
+49 (0) 551 5060 660
Fax:
+49 (0) 551 5060 464
E-mail:
micro@zeiss.de
www.zeiss.de
Number of this Operating Manual: B 46-0084 e
Date of issue: Version 2; 04/01/2006
2
B 46-0084 e 04/06
MIRAX DESK
CONTENTS
Carl Zeiss
CONTENTS
Seite
1
INTRODUCTION ..................................................................................................................5
1.1
Notes on instrument safety ...................................................................................................5
1.2
Notes on warranty ................................................................................................................8
2
DESCRIPTION OF DEVICE....................................................................................................9
2.1
Overall view of MIRAX DESK .................................................................................................9
2.2
MIRAX DESK, basic unit ......................................................................................................10
2.3
Name and intended use ......................................................................................................11
2.4
Instrument description and main features............................................................................11
2.5
Warning and information labels ..........................................................................................12
2.6
System overview of MIRAX DESK single user solution ..........................................................13
2.7
System overview of MIRAX DESK network solution..............................................................14
2.8
Objectives, cameras and adapters........................................................................................15
2.9
Accessories .........................................................................................................................16
2.10
Power supply and light source .............................................................................................16
2.11
Technical data.....................................................................................................................17
3
START UP ..........................................................................................................................20
3.1
First start up........................................................................................................................20
3.2
3.2.1
3.2.2
3.2.3
3.2.4
3.2.5
3.2.6
3.2.7
Device connections and controls .........................................................................................20
Basic unit ............................................................................................................................20
24V power supply ...............................................................................................................21
Monitor ..............................................................................................................................21
Control computer ...............................................................................................................22
Sony DFW-X710 Fire Wire camera .......................................................................................23
Wiring of device components..............................................................................................23
Door ...................................................................................................................................23
3.3
Switching the device on ......................................................................................................24
3.4
Switching the device off......................................................................................................24
4
OPERATION.......................................................................................................................25
4.1
4.1.1
4.1.2
Preparing and loading slides................................................................................................25
Affixing the barcode sticker to the slide...............................................................................25
Inserting and removing a slide.............................................................................................26
4.2
Digitization of a slide (standard procedure)..........................................................................27
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3
Carl Zeiss
CONTENTS
MIRAX DESK
5
SOFTWARE DESCRIPTION ................................................................................................ 28
5.1
Software version ................................................................................................................. 28
5.2
Starting / exiting MIRAX control software............................................................................ 28
5.3
5.3.1
5.3.2
5.3.3
5.3.4
5.3.5
5.3.6
5.3.7
Manual scan ....................................................................................................................... 29
Normal work tab................................................................................................................. 30
Preview tab ......................................................................................................................... 35
Focus tab ............................................................................................................................ 37
Service tab .......................................................................................................................... 39
Display field for live images or camera images ..................................................................... 41
Toolbar ............................................................................................................................... 43
Preview image..................................................................................................................... 44
6
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE............................................. 45
6.1
Instrument care................................................................................................................... 45
6.2
6.2.1
6.2.2
6.2.3
6.2.4
Troubleshooting.................................................................................................................. 46
Removing and reattaching the device cover......................................................................... 48
Changing fuses on the power supply .................................................................................. 49
Changing the halogen lamp................................................................................................ 50
Removing the slide.............................................................................................................. 51
6.3
Requesting service............................................................................................................... 52
4
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MIRAX DESK
INTRODUCTION
Notes on instrument safety
1
INTRODUCTION
1.1
Notes on instrument safety
Carl Zeiss
The MIRAX DESK has been designed, produced and tested in conformity with the standards EN 61010-1
(IEC 61010-1) and IEC 61010-2-101, safety requirements for electrical equipment for measurement, control
and laboratory use, and the relevant requirements laid down in Annex 1 of EC Directive 98/79/EC.
The conformity to the cited EC Directive 98/79/EC is documented by the
sign.
This operating manual includes information and warnings that must be observed by the user.
The following warning and information symbols are used in this manual:
NOTE
This symbol refers you to advice that you must observe under all circumstances.
CAUTION
This symbol indicates a possible hazard to the instrument or system.
CAUTION
This symbol indicates a possible hazard to the user of the instrument.
CAUTION
Hot surface!
CAUTION
Disconnect the instrument from line power before opening it!
CAUTION
Risk of jamming the fingers!
B 46-0084 e 04/06
5
Carl Zeiss
INTRODUCTION
Notes on instrument safety
MIRAX DESK
The MIRAX DESK, including original accessories, must not be used for applications other than those
described in this manual.
Pay special attention to the following information:
The manufacturer cannot assume any liability for other applications of the instrument, including
those of individual modules or single parts. This also applies to any service or repair work that is
not carried out by authorized service personnel. In case of non-compliance, all warranty claims
shall be forfeited.
The instrument must not be operated in explosive environments.
When working with hazardous substances, observe the directions for intended and correct use,
and the relevant statutory safety precautions.
The power plug must be inserted in an outlet with a grounding (earth) contact. The protective
capacity must not be rendered ineffective by using an extension cable without a ground wire.
If it is determined that protective measures are no longer effective, the instrument must be
switched off and secured against inadvertent operation. Please contact a Zeiss service agency or
the Carl Zeiss Microscopy Service to have the instrument repaired.
The MIRAX DESK is powered by a power supply allowing line voltages to be used in the range
of 100 to 240 V ±10 %, 50/60 Hz without any need to change the voltage setting on the
instrument.
Before switching on the instrument, check whether it is suitable for the available line voltage.
Always disconnect the instrument from the power outlet before opening it and changing the
fuses.
Only use fuses appropriate for the rated current. The use of makeshift fuses and short-circuiting
of the fuse holders are not permitted.
The MIRAX DESK is not equipped with any special devices for protection from corrosive,
potentially infectious, toxic, radioactive, or other substances that may be hazardous to health.
Make sure to observe all legal regulations when handling such substances, particularly the
relevant national accident prevention regulations. Decontaminate infectious materials after use.
Dust and dirt may impair the instrument’s performance. The instrument must therefore be
protected as far as possible from such influences and covered with the dust cover when not in
use. Always verify that the instrument is switched off before you cover it.
Avoid touching the hot lamp bulb. Always pull the power plug before changing the bulbs and
allow the instrument to cool down for some 15 minutes.
6
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MIRAX DESK
INTRODUCTION
Notes on instrument safety
Carl Zeiss
Never open the front door unless prompted accordingly by software. When you open the door
at any other time, make sure not to reach with your hands into the instrument to avoid the risk
of crushing your fingers.
The front door is equipped with an interlock switch. If the door is opened while a digitization
process is running, this process will be stopped instantly.
Clogged or covered ventilation slats may lead to heat build-up that will damage the instrument
and, in extreme cases, cause a fire. Always keep the ventilation slats clear and ensure that no
objects enter the instrument through the ventilation slats.
Defective instruments must not be disposed of with household waste. Dispose of the
instrument in compliance with the relevant legal requirements.
The instrument may only be opened by trained persons or service staff.
The instrument may only be operated by trained personnel who must be aware of the possible
dangers involved with operation and the particular application concerned. The MIRAX DESK is a
high-precision instrument that can be impaired in its performance or destroyed when handled
improperly.
B 46-0084 e 04/06
7
INTRODUCTION
Notes on warranty
Carl Zeiss
1.2
MIRAX DESK
Notes on warranty
The manufacturer guarantees that the instrument is free of defects in material and workmanship when
delivered. Possible defects must be notified to us immediately and steps be taken to minimize damage. If
notified of such a defect, the manufacturer is obligated to rectify it at his discretion, either by repairing
the instrument or delivering an intact replacement. No guarantee is provided for defects caused by
natural wear (wearing parts in particular) and improper use.
The instrument manufacturer shall not be liable for damage caused by faulty operation, negligence or
any other tampering with the instrument, particularly the removal or replacement of instrument
components, or the use of accessories from other manufacturers. Any such action shall lead to a forfeit
of all warranty claims.
With the exception of the work specified in this manual, no maintenance or repair of the MIRAX DESK
may be undertaken. Repairs may only be carried out by Carl Zeiss service staff or persons expressly
authorized by Carl Zeiss. In case of any malfunction of the device, contact the responsible Carl Zeiss
service agency.
8
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MIRAX DESK
DESCRIPTION OF DEVICE
Overall view of MIRAX DESK
2
DESCRIPTION OF DEVICE
2.1
Overall view of MIRAX DESK
1
2
3
4
5
6
Carl Zeiss
24V power supply unit
30"TFT monitor
Mouse
Image Analysis XEON Workstation
Keyboard
MIRAX DESK basic unit
Fig. 2-1
MIRAX DESK, overall view
B 46-0084 e 04/06
9
DESCRIPTION OF DEVICE
MIRAX DESK, basic unit
Carl Zeiss
2.2
1
2
3
4
MIRAX DESK, basic unit
Scanner cover
Protection rails
Sony DFW-X710 scan camera
Door
Fig. 2-2
10
MIRAX DESK
MIRAX DESK, basic unit
B 46-0084 e 04/06
MIRAX DESK
2.3
DESCRIPTION OF DEVICE
Name and intended use
Carl Zeiss
Name and intended use
Manufacturer’s designation: MIRAX DESK
The MIRAX DESK digitizes biological patterns and specimens, and samples of human and animal tissue on
smooth slides in transmitted light.
Typical fields of application of the instrument include e.g.:
−
−
−
−
Hospitals
Medical practices
Universities
Industry
2.4
Instrument description and main features
The main features of the MIRAX DESK are:
− Robust and stable basic unit
− Intuitive operation via keyboard and mouse
− Manual loading of one specimen slide each
− Illuminator HAL 12 V, 5 W for transmitted light
− High-resolution scanner optics
− Identification of the slide by digitization of barcode area
− Automatic specimen recognition and digitization of detected objects on the slide
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11
Carl Zeiss
DESCRIPTION OF DEVICE
Warning and information labels
2.5
Warning and information labels
Fig. 2-3
Location of warning and information labels
12
MIRAX DESK
B 46-0084 e 04/06
MIRAX DESK
2.6
DESCRIPTION OF DEVICE
System overview of MIRAX DESK single user solution
Carl Zeiss
System overview of MIRAX DESK single user solution
B 46-0084 e 04/06
13
Carl Zeiss
2.7
14
DESCRIPTION OF DEVICE
System overview of MIRAX DESK network solution
MIRAX DESK
System overview of MIRAX DESK network solution
B 46-0084 e 04/06
MIRAX DESK
2.8
DESCRIPTION OF DEVICE
Objectives, cameras and adapters
Carl Zeiss
Objectives, cameras and adapters
Illustration
Description, technical specifications
The standard MIRAX DESK is equipped with a Plan-Apochromat
20x / 0.8 objective.
Plan-Apochromat 40x / 0.95 Korr objective (optional)
Sony DFW-X710 Fire Wire
Without illustration
Preview camera with LED white light background illumination and
incident light illumination
Without illustration
The standard MIRAX DESK is equipped with a C-mount adapter 1x.
Without illustration
C-mount adapter 0.63x (optional)
B 46-0084 e 04/06
15
DESCRIPTION OF DEVICE
Accessories
Carl Zeiss
2.9
Accessories
Illustration
2.10
Illustration
16
MIRAX DESK
Description, technical specifications
Catalog No.
Barcode stickers
415000-0101-000
Power supply and light source
Description, technical specifications
Catalog No.
24V power supply
–
HAL 12 V, 5 W lamp
for standard equipment, transmitted light
000000-0467-436
B 46-0084 e 04/06
MIRAX DESK
2.11
DESCRIPTION OF DEVICE
Technical data
Carl Zeiss
Technical data
Dimensions (width x depth x height)
MIRAX DESK, basic unit ....................................................................approx. 267 mm x 500 mm x 252 mm
Control computer .............................................................................approx. 206 mm x 560 mm x 440 mm
19” monitor .....................................................................................approx. 420 mm x 240 mm x 490 mm
24V power supply........................................................................... approx. 170 mm x 300 mm x 110 mm
Weight
MIRAX DESK, basic unit ........................................................................................................ approx. 11 kg
24V power supply................................................................................................................... approx. 2 kg
Ambient conditions
Transport (in packaging)
Permissible ambient temperature ......................................................................................-40 °C to +70 °C
Storage
Permissible ambient temperature .....................................................................................+10 °C to +40 °C
Permissible relative humidity (no condensation)............................................................ max. 75 % at 35 °C
Operation
Permissible ambient temperature .....................................................................................+10 °C to +40 °C
Permissible relative humidity ........................................................................................ max. 75 % at 35 °C
Altitude at installation site ..................................................................................................... max. 2000 m
Atmospheric pressure ................................................................................................ 500 hPa to 1060 hPa
Pollution degree ......................................................................................................................................2
Operating data
Intended site..........................................................................................................................closed rooms
Electrical protection class .......................................................................................................................... I
Protection type .................................................................................................................................. IP 20
Electrical safety ......................................................... in compliance with EN 61010-1 (IEC 61010-1) under
consideration of CSA and UL directives
Overvoltage category............................................................................................................................... II
Radio interference suppression ......................................................... in accordance with EN 55011 Class B
Noise immunity.............................................................................................in accordance with EN 61326
Input voltage, basic unit......................................................................................................... 24 V DC ±1 V
B 46-0084 e 04/06
17
Carl Zeiss
DESCRIPTION OF DEVICE
Technical data
MIRAX DESK
24V power supply
Intended site .........................................................................................................................closed rooms
Electrical protection class .......................................................................................................................... I
Protection type .................................................................................................................................. IP 20
Line voltage..................................................................................................................... 100 to 240 V AC
Line frequency ............................................................................................................................ 50 / 60 Hz
Power consumption................................................................................................................. max. 50 VA
Secondary voltage ......................................................................................................................... 24 V DC
Secondary current ........................................................................................................................max. 3 A
Fuses ....................................................................................................... 2 x T 2.5 A / H, 250 V, 5 x 20 mm
Light source
Halogen lamp (transmitted light).......................................................................................... HAL 12 V, 5 W
Average service life of halogen lamp (000000-0467-436) ............................................................... 4000 h
Software
MIRAX Viewer (optional) ...............................................................................................415010-0103-000
MIRAX Viewer 3D Module (optional) .............................................................................415010-0102-000
MIRAX Server (optional).................................................................................................415010-0108-000
MIRAX DESK, standard configuration
Objective ..........................................................................................................Plan-Apochromat 20x / 0.8
Camera ............................................................................................................. Sony DFW-X710 Fire Wire
Camera adapter .........................................................................................................C-mount adapter 1x
Slide identification ................................................................................. Storage of barcode area as image
Option: Interpretation of barcode label as character string; the following 1D and 3D barcode
types can be interpreted:
BC412 (standard encoding type); Codabar (standard encoding type); Code39 (ASCII
encoding); Code93 (ASCII encoding); Code128 (UCC/EAN128) (ASCII encoding); DataMatrix
(numeric encoding, Alpha encoding, AlphaNumericPunc encoding, AlphaNumeric encoding,
ASCII encoding, IS08 encoding); EAN8 (numeric encoding); EAN13 (Numeric encoding);
Interleaved 2/5 (numeric encoding); Maxicode (encoding modes 2, 3, 4, 5, 6); MicroPDF417
(standard encoding type); PDF417 (standard encoding type); Pharma (numeric encoding);
Planet (numeric encoding); Postnet (numeric encoding); QR (QR code Model 1, 2 encoding);
RSS (RSS 14, RSS 14 Stacked, RSS 14 Stacked Omni, RSS 14 Truncated, RSS Expanded, RSS
Expanded Stacked; RSS Limited encoding); UPC-A (numeric encoding); UPC-E (numeric
encoding)
18
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MIRAX DESK
DESCRIPTION OF DEVICE
Technical data
Carl Zeiss
Slide specifications
Dimensions:............................................(24.6 to 26.0) mm x (75.0 to 76.2) mm; 0.9 to 1.2 mm thickness
........................................................................................................ recommended: colored barcode area
Image digitization
1/3" type progressive SCAN IT CCD
Pixel size ....................................................................................................................... 4.65 µm x 4.65 µm
Pixel resolution with 20x objective and C-mount adapter 1x ........................................................ 0.23 µm
Control computer, system requirements
CPU..................................................................................................................................2 x Intel 2.6 GHz
RAM...................................................................................................................................................2 GB
Hard disk ........................................................................................................................................130 GB
Connections ...........................................................................................................................2 x Fire Wire
............................................................................................................................................... 1 x USB 2.0
.............................................................................................................................................. GB Ethernet
Input devices .................................................................................................................. keyboard, mouse
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19
START UP
First start up
Carl Zeiss
3
START UP
3.1
First start up
MIRAX DESK
The basic unit is delivered packed in a wooden box and a polyethylene box with cardboard packaging.
Additionally, the following components are supplied separately: power supply, scan camera, 1x C mount
adapter, cables (2x Fire Wire, 1x USB) and spare lamps.
Control computer and monitor are delivered in a separate cardboard packaging.
Installation and first start up of the MIRAX DESK are carried out by Carl Zeiss service personnel who will
hand over the instrument in a fully operational condition.
Dispose of the original packaging properly or save it for storing the device during extended periods of
non-use or returning it to the manufacturer.
3.2
Device connections and controls
3.2.1
Basic unit
The connection panel at the rear of the instrument
contains the socket for the power supply cable
(+24 V DC) (3-1/1) of the 24V power supply unit
(24 V) with power indicator LED (3-1/2) and the
socket for the trigger cable (3-1/3) for the scan
camera.
In addition, the MIRAX DESK has a port for the
MIRAX DESK USB cable (3-1/4) and a Fire Wire port
for the preview camera (3-1/5) to establish
connection to the control computer.
Fig. 3-1
20
Connectors on basic unit
B 46-0084 e 04/06
MIRAX DESK
3.2.2
START UP
Device connections and controls
Carl Zeiss
24V power supply
The power supply provides the basic unit with
24 V DC.
The power switch (3-2/1) is located at the front of
the power supply.
The back panel contains the line power input
connector
(3-2/3)
with
integrated
fuse
compartment and the non-detachable power
supply cable (3-2/2) for connection to the basic
unit.
Fig. 3-2
3.2.3
Controls and connectors on 24 V
power supply
Monitor
Please consult the supplied separate manual for information on the connectors and the location of the
controls of the monitor.
B 46-0084 e 04/06
21
START UP
Device connections and controls
Carl Zeiss
3.2.4
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
Control computer
Fire Wire port
DVD drive (RW)
DVD drive
3.5" disk drive
ON/OFF switch
2x USB port
Power input connector
Keyboard connector
Mouse connector
4x USB ports – Use one of these ports to establish the connection to the MIRAX DESK basic unit
Fire Wire port – To be used for the connection of the scan camera
Network connector
DVI port
DVI port – To be used for connection of monitor
Fire Wire port (separate card: 000000-0452-036) – To be used for connection to preview camera
Fig. 3-3
22
MIRAX DESK
Connectors and controls of control computer
B 46-0084 e 04/06
MIRAX DESK
3.2.5
START UP
Device connections and controls
Carl Zeiss
Sony DFW-X710 Fire Wire camera
The Sony DFW-X710 Fire Wire serves as scan camera.
The connectors for the trigger cable (3-4/1) and the video output (3-4/2), Fire Wire (IEEE 1394/iLink), are
located at the back of the camera.
Fig. 3-4
Connectors on Sony camera
3.2.6
Wiring of device components
Establish the cable connections of the system using the supplied cables and the connectors shown in
Fig. 3-1, Fig. 3-2, Fig. 3-3 and Fig. 3-4.
Verify that all cable connections are tightly fitted.
Never connect defective cables, but contact Zeiss Service for a replacement.
3.2.7
Door
At the front of the device, a door is located used for loading a specimen slide (4-3/1). When this door has
been pushed aside to the left, a slide can be loaded or removed, if this process was initiated via software
before.
The front door is equipped with an interlock switch, which instantly stops a running digitization
process when this door is opened.
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23
START UP
Switching the device on
Carl Zeiss
3.3
MIRAX DESK
Switching the device on
To power all system components, switch on 24 V power supply, control computer and monitor
separately.
• First, switch on the 24 V power supply at the power switch (3-2/1).
• Switch on the control computer by pressing its ON/OFF switch (3-3/5).
The operating system is being started.
• Switch on the monitor.
The device is now ready for operation.
3.4
Switching the device off
• Exit MIRAX DESK software.
• Shut down the control computer following the standard Windows procedure.
• Switch the 24 V power supply off at the ON/OFF switch (3-2/1).
• Switch off the monitor.
24
B 46-0084 e 04/06
OPERATION
Preparing and loading slides
MIRAX DESK
4
OPERATION
4.1
Preparing and loading slides
Carl Zeiss
You may use all slides and coverslips, which meet the following specifications:
Slides
Coverslips
Length:
75.0 to 76.2 mm
Length:
max. 50 mm
Width:
24.6 to 26.0 mm
Width:
max. 24 mm
Thickness:
0.9 to 1.2 mm
Thickness: 0.13 to 0.19 mm
The slides should have a colored area for the use of barcodes.
Thus, all slides may be used manufactured in compliance with the International Standard
ISO 8037/1.
The edges of slide and coverslip should be parallel.
When mounting the coverslip, care should be taken, that no part of the coverslip juts out
beyond the slide.
4.1.1
Affixing the barcode sticker to the
slide
The barcode field (4-1/3) is captured by the
preview camera and displayed in software. When
affixing the label, take care to leave a blank margin
of 1 to 2 mm around relative to the barcode area.
The interpretation of the barcode as text string
requires the use of a software option that must be
purchased separately.
• Take the barcode sticker from the roll and –
taking care that it is crease-free – affix it to the
colored area (4-1/3) as shown in Fig. 4-1.
• Affix the barcode sticker (4-1/4) to the specimen
side (holding the coverslip) (4-1/2) of the slide
(4-1/1).
Fig. 4-1
Slide with barcode sticker
• The outer edges of the sticker should be
approximately 1-2 mm away from the edge of
the barcode area to ensure that the barcode
can be read reliably.
• The barcode sticker must not overlap the
coverslip and not extend over the edges of the
slide.
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25
OPERATION
Preparing and loading slides
Carl Zeiss
4.1.2
MIRAX DESK
Inserting and removing a slide
The MIRAX DESK features a semi-manual feed for one slide each.
Inserting a slide
• In MIRAX control software, click on the
button (for the use of the software, see p. 28ff).
The specimen holder moves to the load/unload position.
• A message is displayed prompting you to insert
a new slide (Fig. 4-2).
• Open the door (4-3/1) by pushing it to the left.
• Throw the fixing lever (4-3/2) to the left.
• Put the slide (4-3/4) onto the specimen holder
Fig. 4-2
(4-3/3) so that coverslip and label are on top;
then, push it in.
Software prompt to insert a new
slide
Take care that the slide is not canted, and that it rests perfectly horizontally on the stage.
• After insertion of the slide, throw the fixing lever to the right until it touches the slide thus fixing it in
position.
• Close the door by pushing it to the right.
The door closure initiates the reading of the barcode field of the slide and the capture of the preview
image of the specimen area, which is displayed in the control software.
Removing a slide
• By
a click on the
button, the
unloading of the slide is initiated and the
specimen holder moved to the load/unload
position.
• A message window informs you that you can
change or insert the slide now (Fig. 4-2).
• Open the door (4-3/1) by pushing it to the left.
• Throw fixing lever (4-3/2) to the left.
• Remove the slide (4-3/4).
• You
may either insert another slide (as
described above) or throw the fixing lever to the
right as far as it will go and close the door.
Fig. 4-3
Inserting/removing a slide
The front door is equipped with an interlock switch, which instantly stops a running
digitization process when this door is opened.
26
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OPERATION
Digitization of a slide (standard procedure)
MIRAX DESK
4.2
Carl Zeiss
Digitization of a slide (standard procedure)
This flow chart applies to a single slide.
Switch on MIRAX DESK, PC
and monitor (see 3.3)
Start MIRAX control software
Choose Normal work
Click on
(see 5.2.1)
Insert the slide (see 4.1.2)
Preview image is displayed
Choose Preview (see 5.2.2)
Choose Manual thresholding
- Vary threshold value until object is completely masked
- Delete regions not to be digitized (e.g. dirt spots)
Choose Normal work (see 5.2.1)
Enter a slide name
Change Scan options, if necessary
Click on Start scan
Digitization process is
complete
Click on
(see 5.2.1)
Remove the slide (see 4.1.2)
Exit Manual scan
Exit MIRAX control software
Switch off MIRAX DESK, PC and
monitor (see 3.4.)
B 46-0084 e 04/06
Presentation of software elements
Italic + bold
Bold:
Underlined:
Italic:
Scan mode
Tab, dialog box
Button
Selection field
27
SOFTWARE DESCRIPTION
Software version
Carl Zeiss
5
SOFTWARE DESCRIPTION
5.1
Software version
MIRAX DESK
The software description is based on version 1.8 of the control software for the MIRAX DESK. As
software is continuously improved, the screenshots shown in this chapter may differ from the current
version delivered.
5.2
Starting / exiting MIRAX control software
• Double-click on the program icon (Fig. 5-1) on
the desktop.
MIRAX control software is being started.
Fig. 5-1
Program icon of
MIRAX software
• Alternatively, start the software with:
Start f Programs f Carl Zeiss f Mirax
Scan
During program start, all motors, the preview
camera and the scan camera are initialized.
After successful initialization, the program displays
a selection window (Fig. 5-2); as the scanner can
be operated only semi-manually, the Manual
mode is started immediately.
If you had exited the manual mode and want to
reactivate it, click on the Manual button.
Fig. 5-2
28
Mode selection window
If you want to exit the control software, click on
the cross button ( ) in the title bar of the
selection window.
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SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
5.3
Carl Zeiss
Manual scan
The Manual scan mode (Fig. 5-3) allows the digitization of a single slide. By using the functions provided
on the various tabs, you can determine parameters and execute actions:
−
Normal work:
Basic settings for digitization, positioning the stage in the position for
loading/unloading the slide and starting the digitization process
−
Preview:
Settings for optimized preview images
−
Focus:
Settings for focusing processes
−
Service:
Auxiliary functions for operation and maintenance
Live and camera images, information field, toolbar and preview images are always displayed
independently of the selected tab.
2
1
5
3
4
1
2
3
4
5
Dialog and function field for scanner functions and settings (display controlled via the tabs)
Display field for live and camera images
Field providing information on the running digitization process
Toolbar for editing the preview image and for manual positioning of the slide relative to the optical axis
Preview image captured by the preview camera (areas to be digitized appear in red) and image
of the barcode area (display controlled via Preview und Barcode area tabs)
Fig. 5-3
Manual scan dialog and display window
B 46-0084 e 04/06
29
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
5.3.1
MIRAX DESK
Normal work tab
Normal work is the tab for selecting and
activating basic unit functions (Fig. 5-4).
The following functions can be released:
Inserting and removing slides
With the
button, the specimen holder is
moved to the load/unload position. In this position,
you can remove the slide and put in a new one.
For a detailed description, see Section 4.1.2.
Take care that the slide is not canted,
and that it rests perfectly horizontally
on the stage.
Fig. 5-4
Normal work tab
The Read barcode function is an option allowing
the image of the barcode area to be interpreted as
character string (barcode). The barcode appears as
text under Slide name. If you did not purchase the
barcode reader option or if no barcode sticker is
affixed to the slide, you may enter the name of the
slide manually in the Slide name input field.
Defining digitization options
A click on the Scan options button opens the
Scan Settings dialog box (Fig. 5-5) for the entry of
parameters for the process of digitization. The
values entered apply to the entire process.
Under Scan settings, you can define the
parameters controlling the resulting quality of the
virtual slide. Fig. 5-5 shows the pre-set defaults.
The parameters selectable in this window are
described in the following two tables:
Fig. 5-5
30
Scan Settings
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MIRAX DESK
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
The table below details the individual parameters of the Scan Settings:
Parameter
Explanations
Image compression
Selection of the image format for the individual image fields
JPG
The images are saved as Joint Photographic Experts Group files (jpeg) with
losses in image quality. Using the quality slide control, you can adjust the
resulting image quality. Great values result in images of high quality but large
files; small values result in images of low quality and small files. The pre-set
default of 90 % is the best compromise between image quality and file size,
but also lower values (e.g. 50 %) will deliver images of good quality.
PNG
The images are saved as Portable Network Graphics files (png). This method
ensures lossless storage of image information. PNG was made a standard in
2003 W3C (ISO Standard ISO/IEC 15948:2003); see also BMP.
BMP
The images are stored without any loss as Windows Bitmap files (bmp). As
the compression algorithm used for BMP files is less efficient than that for
PNG files, the size of BMP files is larger than that of PNG files.
Fine focus frequency
With this slider button, you can choose after how many images in succession
the device shall perform fine focusing (focusing over a small Z range in a few
steps). This parameter has an effect on the speed of the digitization process
as the time-consuming fine-focusing process will be performed only after the
number of captured images specified here.
The default setting of 4 is usable for most of the specimens. If a specimen is
very uneven, it is advisable to choose a smaller value. The same refers to
cytological specimens. If you set the slider to 1, fine-focusing is carried out
for every image field. This results in 100 % focused image sequences,
however, the digitization time is considerably increased that way.
Coarse focus frequency
With this slider button, you can choose after how many images in succession
the device shall perform coarse focusing (focusing over a large Z range in
several steps). This parameter has an effect on the speed of the digitization
process as the time-consuming coarse-focusing process will be performed
only after the number of captured images specified here. Coarse focusing
takes longer than fine focusing.
The default setting of 8 is usable for most of the specimens. To this
parameter, the same statements apply as those made above on the
Fine focus frequency. As a rule, it is advisable to choose a value that is
twice as large as the value selected for the Fine focus frequency.
Scan map dilation (µm)
With this value, the red-masked area can be dilated.
The dilation of the marginal zone is specified in µm. The default setting is
250 µm.
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31
Carl Zeiss
SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
Minimal object level (%)
The device focuses only those image fields, in which a structure is detected.
This value defines from which filling level of the image the system shall focus
the specimen. The default setting is 10 %.
With specimens having a poor contrast (e.g. hemalaun/eosin stain of
myocardial cells), it may happen that the complete virtual slide is not
correctly focused as the device fails to detect an object in any of the image
fields. In this case, you are advised to set this value to 0%.
Stitching
When you activate the Stitching option, the individual tiles of the digitized
slide border on each other seamlessly without any overlaps or underlaps
being visible in the MIRAX VIEWER.
If this function is activated, image fields are digitized automatically with a
greater overlap; this will result in an increase in the digitization time (approx.
27 % compared to the function being deactivated) and in larger files. If this
function is not activated, the optimum digitization time and file size are
obtained, but in the resulting image, overlaps and underlaps may appear.
Save image in original
Bayer size
If this option is activated, the individual images are stored with the native
pixel resolution (0.23 µm per pixel in standard configuration). If the function
is deactivated, the pixel resolution (0.32 µm per pixel in standard
configuration) will be reduced. If deactivated, the resulting files are smaller
(by approx. 40 %).
Use saved compensation
image
If this option is deactivated, the system will automatically capture several
reference images of blank areas of the slide for white balance and shading at
the beginning of the digitization process.
However, if the slide is almost fully covered by the specimen (e.g. smears) the
automatic search for blank areas may not always be successful. If this option
is activated, it is possible to use a reference image captured earlier (see
Section 5.3.4) as compensation image, with which the digitized image is
balanced.
32
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SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
Carl Zeiss
The table below provides detailed explanations on the parameters Create hierarchical path for slides and
Scan AutoScope compatible slide:
Parameter
Explanations
Create hierarchical path
for slides
If this option is activated the selected name of the slide or its barcode is
saved to the entered path subdivided in groups of two.
Example:
Slide name: 080412
Slide directory path: C:\Slides
Complete path of the digitized slide:
C:\Slides\08\04\080412\
If this function is deactivated, there is no such subdivision made; for the
above example, the save path would be:
C:\Slides\080412\
Scan AutoScope
compatible slide
This checkbox must be activated if the output format shall be compatible
with different image analysis software packages (not necessary for
AxioVision). With this option being activated, the presentation process in
MIRAX VIEWER is slowed down, i.e. this option should be activated only if
absolutely necessary for subsequent specific image analysis.
Under Slide directory path, enter the target folder for the digitized slides. Selection of the desired folder is
conveniently done in a selection window brought up by a click on the Browse button.
All the parameters entered are saved and recalled, when you restart the software.
After the entry of all desired parameters and file information (see 5.3.2 and 5.3.3), you can initiate the
process of digitization by a click on the Start scan button.
Before starting the digitization process, verify that there is sufficient free disk space on the
target drive! You can easily read the available capacity in the last line of the Current scan
progress information field (Available disk space).
On clicking the Start scan button a message pops up
(Fig. 5-6). To start scanning, confirm the message with OK;
otherwise, click on Cancel.
Fig. 5-6
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Message popping up after
pressing Start scan
33
Carl Zeiss
SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
After the start of the digitization process, the red-marked area of the preview image is digitized
according to the options selected under Scan Settings.
The process of digitization can be stopped at any time by clicking the Stop scan button. This button
becomes accessible only after digitization was started. On activation of this function, the process is
aborted and cannot be resumed at the stop position. On restarting the digitization, the process will be
started again from the beginning.
When the digitization is complete, the popping up Scan complete message informs you that the process
was finished.
34
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MIRAX DESK
5.3.2
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
Preview tab
On the Preview tab in the Preview selection field,
three options are available for defining the
threshold value. This threshold value determines
which areas are to be masked (areas marked red in
software). Only these masked areas will be
digitized subsequently at high resolution:
Automatic thresholding
If you activate this option, the program will run an
automatic algorithm for every preview image in
order to determine the areas to be digitized.
This option can be used for intensively colored
specimens.
Manual thresholding
With this option being activated, you can choose
among the threshold values defined before for
previous images. Lower thresholds will lead to a
higher sensitivity in capturing the areas to be
digitized, which on the other hand means that e.g.
also dirt and other contaminations will be captured
and afterwards digitized. Higher values mean a
reduced sensitivity to contaminations. On the other
hand, it may happen that in this case the system
fails to mask specimen areas of interest that will
thus not be included in the subsequent digitization
process.
Fig. 5-7
Preview tab
If the preset thresholds will not deliver satisfactory
results, but you do not want to change the existing
thresholds, you can set a new threshold via Manual
thresholding/New. You can choose any desired
name and value for this new threshold, which will
then be added to the list.
1
You may choose the desired threshold also directly
by selecting the corresponding name and by
editing the value using the arrow keys of the
Current threshold list box.
This option is recommended for stains of weak
intensity (e.g. immunohistochemical stains).
Use saved preview
Using the buttons of the toolbar (Fig. 5-16), the
user can select areas to be digitized and areas not
to be digitized.
default
1
high
low
masking
Edges of coverslip
Fig. 5-8
Threshold values and masking
In the Saved preview field, you can save a mask
just defined using the Save button or reload it later
by means of the Load button.
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35
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
MIRAX DESK
This option is suitable for slides holding small individual
objects, e.g. some cytological specimens, which cannot be
resolved satisfactorily by the preview camera.
Depending on the set threshold, in the preview image the
edges of the coverslip are detected as objects to be
digitized (see Fig. 5-8; preview image with “low” caption).
As the digitization of these edges is time-consuming and
occupies unnecessary capacity on the hard disk, these
areas can be excluded from digitization using the
Cutting out the coverslip edge option. If this option is
activated, a purple frame is overlaid to the slide (5-9/1). As
the position of the coverslips normally is reproducible, this
function ensures that only those masked areas are digitized
that lie within a freely definable area (rectangle), whereas
unwanted areas will not be digitized although they would
be masked.
You can increase or reduce the size of the rectangle by
means of the mouse. To this end, move the pointer onto
one of the lines, click the mouse button and change the
size as wanted. All formerly red marked objects outside
this frame will be hidden. That way, you have defined that
only the masked objects within this area will be digitized.
2
1
If the Filling holes on scanmap option is activated, all so far
non-masked regions within a masked area, will be masked
as well. This function is helpful with specimens having a
contrast that decreases from the outside to the inside.
When using the default settings for such objects it may
happen, that the weakly contrasted inner region (e.g.
adipose tissue) is not recognized as region to be digitized.
This function ensures that these regions, too, are included
in the digitization process.
If the activation of the above options will not do to
recognize a specimen feature as one to be digitized (e.g. if
contrast is extremely poor), you have the possibility to
encircle the feature with a black marker pen. With the
Scanning area inside the marker option being active, the
area inside the line drawn with the marker will be marked
red and later digitized.
1
Frame (all regions outside the frame
will not be digitized; e.g. the edges of
the coverslip)
2
Edges of coverslip
Fig. 5-9
36
Cutting out the coverslip
edge option
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MIRAX DESK
5.3.3
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
Focus tab
Using the options on the Focus tab (Fig. 5-10), the focus can be readjusted as required during the
scanning process. Besides, you can work offline with the focus.
In the Live field, you can activate the display of a live image of the scan camera by a click on the Live
button. The image will then appear in the field for live and camera images (see Section 5.3.5). This option
is of no effect during the digitizing process, i.e. no live image will be displayed. To this end, the
Show image option on the Service tab must be
activated.
In the Focus field, the focusing range can be
limited. If the Limiting the range option is
activated, the Lower limit and the Upper limit of
the focusing range can be set by clicking on the
corresponding arrow keys.
This function should be applied, if the system does
not focus on the object to be digitized, but on an
imperfection on the coverslip, caused e.g. by dirt
on the coverslip surface.
The Set current button can be used to set the
Lower limit value of the focusing range to the
current focus position (here: Z pos.: 1584) –32 and
the Upper limit value to the current focus position
+32.
Clicking the Auto focus button initiates the
focusing process at the place of the image cursor.
When you use the Auto focus
function, the live image should be
switched off, as the display of the live
image always reduces the processing
speed of all other functions initiated.
Fig. 5-10
Focus tab
The step size of manual focusing can be set with the Step size slider button by means of the arrow
buttons.
Manual focusing is performed by clicking the vertical blue arrow buttons. This is of no effect on the
automatic digitization of specimen slides.
With the mechanical stage function activated (5-16/9), the slide can be observed as with a microscope
provided that the Live button was activated.
On the Focus tab, you can also activate the Extended focusing function. This function is optionally
available.
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37
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
MIRAX DESK
When this function has been activated, several images of different focal planes will be captured for every
image field. The most contrasty regions of the respective images are combined to a single image, which
will then contain the maximum contrast over all images captured. This procedure ensures the maximum
depth of focus. The function is of no effect on the file size, but only on the speed of digitization, as
several images have to be captured and computed to a single image.
Once you have activated the function by checking the Extended focusing checkbox, you can define the
number of focal planes by means of the Z level count checkbox. The starting point is always the current
focus value (as defined by the Z value determined by the autofocus function). A value of five means that
for the computation of the resulting image, the image of the current focal plane and two images each of
the focal planes above and of the focal planes below the current focal plane will be captured. For this
reason, you are advised to choose an odd number for the Z level count value.
The image-to-image spacing in the Z axis is determined by the entered Z step size. This size is defined by
the number of motor steps (one motor step corresponds to approximately 0.15 µm). For the values
entered in Fig. 5-10, the procedure shown in Fig. 5-11 below results.
5
Fig. 5-11
38
Illustration of the process for the Extended focus function
(bright areas are unfocused areas; the darker areas are in focus)
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MIRAX DESK
5.3.4
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
Service tab
On the Service tab, you can activate specific
control and maintenance functions.
When you click the Emergency remove position
button, MIRAX DESK moves the specimen holder
to the load/unload position so that you can remove
the slide. The function of this button is analogous
button on the Normal
to that of the
work tab.
If the function fails to move the slide into the
desired position, the slide must be removed by
opening the cover (see Section 6.2.4).
Normally, after this procedure the system should
“know” that there is no slide inserted. If
nevertheless there is the problem that the system
expects a slide to be in the holder, you can click on
the Slide removed manually button to tell the
software that the slide was removed.
Fig. 5-12
Service tab
If the Show image checkbox is activated, the image
field currently being captured will be displayed in
the display field for live and camera images (5-3/2 or 5-14/3).
The Show image option should only be activated in exceptional cases as it slows down the
digitization process.
Normally, at the beginning of the process of
digitization, for every slide several reference images
of blank areas of the slide are automatically
captured for white balance and shading. However,
if you have slides that are almost fully covered by a
specimen (e.g. smears) the automatic search for
blank areas on the slide may not always be
successful. This becomes apparent if e.g. white
balance and shading of the produced virtual slides
do not show the usual quality (e.g. darker marginal
zones).
Fig. 5-13
Prompt to insert a blank slide
Using the Get new compensation image for main camera function it is possible to capture a
compensation image (reference image) beforehand, with which the digitized image fields will be
compensated later.
B 46-0084 e 04/06
39
Carl Zeiss
SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
When clicking the Get new compensation image for main camera button, the user will be prompted to
put in a blank slide (Fig. 5-13) – even if there is a slide inserted already. The inserted slide should
correspond to the slide/coverslip combination used later as exactly as possible. The information message
cannot be confirmed, as the door of the MIRAX DESK must be opened and closed again for this purpose.
By doing so, the procedure of generating a compensation image for the scan camera is initiated. When
this procedure is concluded, the system will display an information window.
To activate this function for digitization, on the Normal work tab under Scan options the user must have
activated the Use saved compensation image option.
If the preview image of the slide does not yield a satisfactory result, you are advised to generate a new
compensation image for the preview camera. The corresponding procedure is initiated by a click on the
Get new compensation image for preview camera button. To conclude this procedure successfully, make
sure that no slide is in the device. If nevertheless a slide should be in the device and the system detects it,
the device will move the slide to the load/unload position and you will be prompted to take it out.
The data under Database are necessary only if the virtual slides shall be linked to a database. This
function becomes accessible only if a database is mounted. The corresponding procedure is described
separately and is not part of this manual manual.
40
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SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
5.3.5
Carl Zeiss
Display field for live images or camera images
In the display field, the live image or the camera image may be shown (Fig. 5-14).
1
1
2
3
2
3
Toolbar
Status bar
Live or camera image
Fig. 5-14
Display field for live or camera images
B 46-0084 e 04/06
41
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
MIRAX DESK
The live image is displayed only if no digitization process is running. To get an image display during
digitization, the Show image option on the Service tab must have been activated.
The software allows various actions to be carried out on the displayed live image, such as zooming,
determination of exposure time, etc., which are accessible via the toolbar (see Fig. 5-15). However, these
options are not available for the camera image during digitization.
1
2
1
3
4
5
6
7
8
2
3
Toolbar
1
2
3
4
5
6
7
8
Zooms in the live image
Zooms out the live image
The live image is displayed with one pixel on the monitor corresponding to one pixel on the camera chip.
Changes the size of the live image so that it fits the size of the display window.
Activates or deactivates the display of the horizontal and vertical scale for the displayed image
Activates red coloration of all overexposed pixels
Starts the recalculation of the exposure time for the scan camera
Activates a dialog box allowing the current live image to be saved as bitmap or JPEG image.
Status bar
1
2
3
Current position of the image cursor in relation to the live image
Red (R), Green (G) and Blue (B) value of the current pixel
Zoom factor of the current live image (1:1 corresponds to a zoom of 1.00)
Fig. 5-15
42
Toolbar and status bar of the display field for live or camera images
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SOFTWARE DESCRIPTION
Manual scan
MIRAX DESK
5.3.6
Carl Zeiss
Toolbar
The toolbar is displayed independently of the selected tab. The most important functions provided by the
toolbar include those for the determination of the areas to be included in or excluded from digitization, if
the digitization area must be selected manually (e.g. with some cytological specimens) or simply if
unnecessary areas (e.g. dirt) shall be deselected. The corresponding functions are described in Fig. 5-16,
items 3 to 8 and 11. It is sensible to use these functions if the Preview tab is selected.
The movement of the slide relative to the optical axis is activated by the mechanical stage function
(5-16/9). The movement is to be controlled by left-clicking with the mouse in the preview image. For
easier navigation in the preview image, you are advised to enlarge the preview image using the scaling
list box (5-16/10). The current position is indicated by a yellow cross. To deactivate this mode, click on
one of the symbol buttons (5-16/3, 4, 5, 6 or 7).
1
1
2
3
4
5
6
7
8
9
10
11
2
3
4 5
6
7
8
9
10
11
Coordinates of cursor position (status display)
Selection of the units for the cursor position display (mm or numbering)
Button for drawing of a filled rectangle
Button for drawing a filled circle
Button for free-hand drawing
Button for drawing a filled polygon
Button for drawing a dot
Undo button: Clicking this button allows to undo up to 40 edit actions to the preview image step by step.
This function allows to place the cursor at any desired place on the slide by a mouse click; if activated, the slide can be
moved continuously in two coordinates relative to the optical axis, analogous to a mechanical stage of a microscope.
Movement in Z direction (focusing) must be performed via the settings on the Focus tab. The function can be deactivated
again by a click on one of the symbol buttons 3 to 7.
Scaling (zoom) of the preview image (selection of preset values via the list box)
Edit mode for selected areas (in combination with symbol buttons 3 to 7):
Not scanned:
The selected areas are deleted from the preview image, i.e. excluded from scanning
Scanned:
The selected areas will be added to those to be scanned
Fig. 5-16
Toolbar for the selection of areas in the preview image
B 46-0084 e 04/06
43
SOFTWARE DESCRIPTION
Manual scan
Carl Zeiss
5.3.7
MIRAX DESK
Preview image
The preview field shows two preview images depending each on the activated tab:
Preview tab
Barcode area tab
Fig. 5-17
Fig. 5-18
Preview image of specimen
Display of the image of the specimen with the red
mask for the presentation of the area to be
digitized and the purple frame if the
Cutting out the coverslip edge option is activated.
44
Preview image of barcode area
Display of the image of the barcode area. If the
writing should appear upside down, this image
may be rotated by 180° on activation of the
Rotate it by 180 degree option. The image is
saved along with the digitization data and is also
available in MIRAX VIEWER.
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MIRAX DESK
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Instrument care
6
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
6.1
Instrument care
Carl Zeiss
Care of the MIRAX DESK is restricted to the following operations:
• Switch off the instrument each time after use and place the instrument cover over it to protect it from
dust and humidity.
• Do not set up the instrument in a humid room; maximum humidity < 75 %.
• Remove water-soluble dirt (coffee, cola, etc.) by blowing on it and wiping it off with a lint-free cotton
cloth or a cloth moistened with water, to which a mild detergent may be added.
• Wipe off stubborn, oily or fatty dirt, e.g. fingerprints, with a cotton swab or lint-free cotton cloth and
the optics cleaning solution L.
This cleaning solution consists of 90 vol% gasoline and 10 vol% isopropanol (IPA). The individual
components are also known as:
Gasoline:
Medical alcohol, petroleum ether
Isopropanol:
2-propanol,
Dimethylcarbinol,
2-Hydroxypropane
When using MIRAX DESK in humid climatic zones, ensure that the device is kept in a light, dry and wellventilated room with a humidity of < 75 %.
The risk of fungus growth on opto-mechanical instruments invariably exists in the following conditions:
−
Relative humidity > 75 % and temperatures between +15 °C and +35 °C for more than three days.
−
Installation in dark rooms without air circulation.
−
Dust deposits and fingerprints on optical surfaces.
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45
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Troubleshooting
Carl Zeiss
6.2
MIRAX DESK
Troubleshooting
Problem
Cause
The MIRAX DESK does not Power supply is
work.
interrupted.
Remedy
24V power supply has not been switched on.
• Switch on the power supply.
Control computer has not been switched on.
• Switch on the control computer.
• Check the cable connections.
Control software has
stopped.
Slide is jammed
and cannot be
removed through
the door.
• Exit the control software.
• Switch off control computer and 24V power supply;
disconnect the power cable.
• Remove the device cover (see page 48).
• Remove the stuck slide by hand (see page 51).
• Reattach the device cover.
• Re-connect the power cables of 24V power supply
and control computer.
• Switch on the 24 V power supply.
• Switch on the control computer.
• Start the control software.
Error message during
calibration of the scan
camera
The name of the
slide currently to
be digitized exists
already and the
respective file has
been opened in
MIRAX VIEWER.
• Close the virtual slide in MIRAX VIEWER.
Halogen lamp is
defective.
• Replace the halogen lamp (see page 50).
Image is black
The virtual slide is almost
completely out of focus.
Inappropriate value • Set the Minimal object level (%) to 0.
selected for
Minimal object
level (%)
Poor image quality
Imaging error
46
• Contact a Zeiss service representative.
B 46-0084 e 04/06
MIRAX DESK
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Troubleshooting
Carl Zeiss
Virtual slides show poor
stitching in MIRAX
VIEWER:
Scan camera not
adjusted correctly
(e.g. by slackening
of the holder)
• Contact a Zeiss service representative.
Slide is often incorrectly
positioned.
Guiding fault in
transport system
• Contact a Zeiss service representative.
Preview image is too
bright (glared) or too dark
Invalid
compensation
image for preview
camera
• Capture a new compensation image for the preview
camera (see 5.3.4)
Digitization process is
extraordinarily slow
Extended focusing
option is activated
• Deactivate the Extended focusing option if it is not
needed.
Image is unsharp.
Focus range limit
activated
• Deactivate
Fig. 5-10).
the
Limiting the range
option
(see
Wrong focus range • Change the focus range.
setting
All individual images show Algorithm cannot
a shading effect.
find any blank
image fields.
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• Capture a reference image with a blank slide (see
5.3.1, Use saved compensation image and 5.3.4).
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CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Troubleshooting
Carl Zeiss
6.2.1
MIRAX DESK
Removing and reattaching the device cover
The cover of the MIRAX DESK must be removed if you want to replace the halogen lamp or take out a
jammed slide that caused a stop of the digitization process.
The device may only be opened by instructed persons or service technicians.
Before removing the cover, exit the control software, switch off the control computer and
the 24V power supply and disconnect the power cables.
Fig. 6-1
Removing/reattaching the device cover
• Using a Phillips® screwdriver (6-1/3), unscrew both Phillips® screws (6-1/2) from the holes (6-1/1)
located at the sides.
• Then, carefully push the cover (6-1/5) horizontally frontward with light pressure until it can be
removed completely from the basic unit (6-1/4).
• When you have finished maintenance or repair work, reattach the cover. To this end, push it
horizontally towards the basic unit (6-1/4) until the two protruding guide pins (6-1/6) fit into the
corresponding recesses of the basic unit.
• Note that the rear part of the cover must be pushed over the top part of the blue basic unit (6-1/7).
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MIRAX DESK
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Troubleshooting
Carl Zeiss
• To lock the cover in place, insert the screws again and fasten them.
Take care that no cables are squeezed when you attach the cover.
6.2.2
Changing fuses on the power
supply
Make sure to disconnect the power
cable before changing any fuse.
The power input connector with fuse compartment
is located at the back of the 24V power supply
unit. The latter contains two T 2.5 A/H / 250 V
fuses.
• Switch off the power supply and disconnect the
power cable.
• Pull out fuse holder (6-2/3) frontward. Use a
small screwdriver for this purpose, if necessary.
• Remove the fuses (6-2/2) from the fuse holder
and replace them by new fuses.
Fig. 6-2
Changing fuses
• Push the fuse holder (6-2/3) back into the fuse
compartment (6-2/1) as far as it will go.
• Reconnect the power cable and switch on the
power supply unit.
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49
Carl Zeiss
6.2.3
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Troubleshooting
MIRAX DESK
Changing the halogen lamp
• Exit control software, shut down the computer, switch it off and disconnect the power cable.
• Switch off the power supply and disconnect the power cable.
• Remove the cover (see 6.2.1).
Fig. 6-3
Changing the halogen lamp
• Hold the lamp holder (6-3/1) with your hand.
• Loosen the knurled screw (6-3/2) at the underside of the lamp holder as much as is necessary to easily
pull out the lamp holder frontward. Do not remove the screw completely to prevent it from falling into
the device. Screwing it back into place may then be difficult to do.
Caution – Hot surface!
Wait for the lamp surface to cool down.
Keep a cool-down time of approximately 15 minutes.
• Remove the defective lamp (6-3/3) and replace it by a new one (000000-0467-436).
When changing the lamp, avoid touching the replacement bulb with the bare fingers!
• Taking care that the groove at the underside of the lamp holder is correctly aligned, carefully push the
lamp holder into the heat sink as far as it will go. Finally, lock it in place by means of the knurled
screw.
• Reattach the cover (see 6.2.1).
• Plug the power cable of the power supply into the power outlet and switch on the power supply.
• Connect the power cable of the control computer to the power outlet and switch on the computer.
• Start the control software.
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MIRAX DESK
6.2.4
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Troubleshooting
Carl Zeiss
Removing the slide
If a slide has got stuck during the process of
digitization, stop the process and, if possible, move
the slide holder to a position where the slide can
be
easily
removed
by
clicking
the
Emergency remove position button (see 4.1.2).
Crushing hazard! Before removing a
slide that got stuck, stop the process
of digitization, exit the software and
switch off the device.
If this is not possible or the slide got broken, follow
this procedure:
• Exit the control software, shut down the control
computer and disconnect the power cable.
• Switch off the power supply and disconnect the
power cable.
Fig. 6-4
Removing the slide
• Remove the cover as described in Section 6.2.1.
• Throw fixing lever (6-4/3) frontward to the left.
• Remove the slide (6-4/1) from the holder (6-4/2). If the slide got broken, make sure to remove all
pieces completely from the device.
• Swing fixing lever (6-4/3) back to the right towards the holder.
• Reattach the cover (see 6.2.1).
• Connect the power cable of the power supply to the power outlet and switch on the power supply.
• Connect the power cable of the control computer to the power outlet and switch on the computer.
• Start the control software.
After every manipulation of mechanical components, you must always perform complete reinitialization
of the device.
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51
CARE, MAINTENANCE, TOUBLESHOOTING AND SERVICE
Requesting service
Carl Zeiss
6.3
MIRAX DESK
Requesting service
Except for the work described in this manual, all repairs to mechanical, optical or electronic components
inside the device and its electrical components may only be performed by Carl Zeiss service staff or
specially authorized specialist staff.
To ensure optimum setting and trouble-free function of your MIRAX DESK over a longer period of time,
we recommend that you enter into a service/maintenance agreement with Carl Zeiss.
For subsequent orders, or when service is required, please contact your local Carl Zeiss representative.
For additional information, contact us at
micro@zeiss.de
or visit us on the Internet at
http://www.zeiss.de
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